CN1063487C - Live yeast fixing method and device - Google Patents
Live yeast fixing method and device Download PDFInfo
- Publication number
- CN1063487C CN1063487C CN95114215A CN95114215A CN1063487C CN 1063487 C CN1063487 C CN 1063487C CN 95114215 A CN95114215 A CN 95114215A CN 95114215 A CN95114215 A CN 95114215A CN 1063487 C CN1063487 C CN 1063487C
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- CN
- China
- Prior art keywords
- viable yeast
- sponge
- carrier
- cage
- solubility
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Immunology (AREA)
- Sustainable Development (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a method for fixing live yeast, a carrier structure made of the live yeast and a device applying the method. A carrier produced by a special method has the structure that the inside of a netted cavity of foamed sponge is supported by a netted gel skeleton made from sponge acid calcium, which is used for embedding live yeast, so the problems of poor washing resistance of a carrier and poor wear resistance are solved, the service life of the carrier is extended, and the service efficiency of the carrier is improved. The present invention solves an important technical problem in the fields of alcohol and beverage production by means of biological fermentation, etc.
Description
The present invention relates to a kind of fixing means and application apparatus thereof of viable yeast.
The fixing means of viable yeast before adopts calcium alginate gel that viable yeast embedding method is wherein done glomeration or membrane carrier, and some adds that also some plastics do the toughener of carrier; For example, Chinese patent CN85105279.The shortcoming that adopts this carrier to carry out the fixed way of viable yeast is the anti-poor performance of washing away and wearing and tearing, and is difficult to by the different process needs at any time, easily changes the shape and the size of carrier.
The purpose of this invention is to provide effectively fixedly viable yeast, make it to be difficult for being washed away, wear and tear and to run off, its viable yeast carrier change shape and size on request easily, the viable yeast effect is brought into play sufficient, viable yeast fixing means and application apparatus thereof.
Viable yeast fixing means of the present invention is: viable yeast is put into the solubility alginate solution, stir, at least one foam sponge (1) put into the mixed solution of above-mentioned viable yeast and solubility alginate solution, make mixed solution infiltrate the intracavity space of sponge (1); Then, take out the sponge (1) of band mixed solution and put into the calcium salt soln that concentration is the solubility of 2%～15% weight ratio, treat sponge (1) internal cavities be settled out be embedded with viable yeast calcium alginate gel (2) afterwards, take out the sponge become viable yeast immobilization carrier (3), clean with clear water.When solubility alginates concentration is lower than 1% weight ratio, be difficult to form gel, when being higher than 5% weight ratio, be difficult to dissolving; When the solubility calcium salt concn is lower than 2% weight ratio, calcium alginate gel (2) moulding is slow excessively, when being higher than 15% weight ratio, to the toxic influence of viable yeast, after the mixed solution of sodium alginate and viable yeast infiltrates the intracavity space of sponge (1), the sponge (1) that will contain mixed solution is put among the solubility calcium salts solution, produces: solubility alginates+soluble calcium salt → calcium alginate gel ↓+reactant salt; This reaction is along with the surface of solubility calcium salts solution from sponge (1) progressively is deep in the internal network cavity of sponge to internal penetration.The shape of the inner fully basis of time sponge of calcium alginate gelization of sponge (1) changes with size; (one, two that can take out in many carriers is cut the way of checking, determines required time substantially); After the inner calcium alginate gelization fully of sponge (1), take out the sponge (1) that has become ferment fixation support alive (3).For example; Volume is 8cm
3The abundant gelation time of square shape sponge that has mixed solution be about 2 hours.
The preferred version of the fixing means of viable yeast of the present invention is, on above-mentioned basis; The solubility alginates is a sodium alginate, and its strength of solution is 1～5% weight ratio, and soluble calcium salt is a calcium chloride, and its strength of solution is 2～10% weight ratios.
The immobilization viable yeast carrier that the fixing means of viable yeast of the present invention produces, its structure is: be filled with embedding in the spumescence network cavity in foam sponge (1) and the calcium alginate gel of viable yeast (2).Sodium alginate in sponge (1) spumescence network cavity is most of or all become calcium alginate gel (2), and embedding is when viable yeast and being filled in the network cavity, and carrier of the present invention (3) just forms.At this moment, calcium hardened marine alga sea calcium gel (2) is supportting the network cavity of sponge (1), and in the network space, the calcium alginate gel (2) that viable yeast in embedding is the network skeleton shape.This skeleton is supporting the network cavity of sponge (1) as the reinforcing bar of cement concrete.Between sponge (1) network cavity wall and gel (2) certain space is arranged, the working fluid of carrier can free in and out carrier (3) by this space, and the fermentative action of viable yeast can be given full play to inside from the surface of carrier (3).Like this, have stronger shock resistance, polishing machine owing to supported the sponge (1) that has hardened by gel (2) network skeleton, protecting viable yeast can because of impact, friction not run off.Needs for different fermentations technology, can select the carrier (3) of different quantities and different shapes size, and, the solid support material sponge (1) that the present invention adopts is easy to cutting, and the hardness of gel (2) network skeleton does not cause big influence to cutting, so no matter in advance with raw material sponge cutting processing, or cutting processing is not difficult again after making carrier.Can easily be processed into different shape and size according to the zymotechnique needs of various products, as A, B, the C shape of Fig. 1.
The application apparatus of the fixing means of viable yeast of the present invention is: a cage (4) that has mesh, immobilization viable yeast carrier (3) is packed in the cage (4), fermentor tank housing (5) inner chamber middle part is fixed, is installed in to cage (4), one feed openings (6) and a phosgene gas discharge hole (7) are arranged at housing (5) top, and a discharge port (8) is arranged at the bottom.Carrier (3) is contained in the cage (4), and (quantity of carrier (3) and shape, size all are to decide according to the specific requirement of zymotechnique.) making concentrated cage (4) lining that constrains in of whole carriers (3), the mesh of cage (4) is designed to less than the carrier bulk of adaptation to the technology of the volume minimum of carrier bulk demand.Feed openings (6) discharge openings (8) is used for the charging of fermentation raw material (for example molasses etc.) of zymotechnique and the discharging of finished product, and (for example: alcohol etc.) gas discharge hole (7) is used for discharging and results from the CO2 gas that produces during the fermentation on the carrier (3).In addition, people's maintenance for convenience can also be opened a hole door with cover that supplies the people to enter on the top of housing (5).Except that the above, utilize fermentation technology that this device carries out and traditional just the same.For producing unnecessary heat energy during the fermentation, can adopt the way of outside housing, drenching water coolant.Also can be with other traditional way.For example: the way of establishing cooling tube in cage (4) lining.
Fig. 1 is three kinds of carrier square section synoptic diagram of the present invention.
Fig. 2 is a device longitudinal profile synoptic diagram of using viable yeast fixing means of the present invention.
The present invention is because utilization solubility alginates and solubility calcium reactant salt produce the sedimentary principles of chemistry of calcium alginate gel, and the inner calcium alginate gel back bone network that forms the embedding viable yeast of sponge that makes the solubility calcium salts solution infiltrate the mixed solution that has solubility alginates and viable yeast in the sponge network inner chamber supports the carrier structure of sponge internal cavity network.The service efficiency and the life-span of carrier have been improved effectively.And again at this carrier design the fermentation unit that is fit to, finished bio-fermentation process and Design of device.Embodiment 1 (utilizing cane molasses to produce alcohol)
One, the making method of carrier (3): as Fig. 1-A
Get 20 milliliters of viable yeast (AS21189 kind) nutrient solutions, (concentration is 100,000,000/milliliter); Get one in foamed artificial sponge, cut into the 125cm of proper amt
2Imitative square shape sponge (1) block; Nutrient solution is added among 100 milliliters of the sodium alginate solns that concentration is 2.5% weight ratio, stir, again sponge (1) is immersed in the mixed solution of sodium alginate and viable yeast, make the network cavity in the mixed solution infiltration sponge (1), taking out the sponge (1) of closing mixed solution then, to put into concentration be that the calcium chloride solution of 5% weight ratio soaked 10 hours; Take out the sponge (1) of the immobilization viable yeast carrier (3) that has become, clean standby with clear water.At this moment the structure of carrier (3) is: be filled with embedding in the spumescence network cavity in foam sponge (1) and the calcium alginate gel of viable yeast (2).
Two, use the fermentation unit of above-mentioned carrier (3), as shown in Figure 2:
A stainless steel cage (4) that has mesh, volume 30M
2Above-mentioned carrier (3) is filled in the inside, and cage (4) is installed in the interior middle part of fermentor tank housing (5), fixes with support.Fermentor tank housing (5) is that a volume is 50M
3Cylinder.One opening for feed (6) and a CO are arranged at top
2Gas discharge hole (7) also has a band cover pore door that advances interior maintenance for the people.One discharge port (8) is arranged at the bottom; Article one, the trepanning on fermentor tank housing (5) wall is passed at two ends, stainless steel cooling pipe road (9) respectively, and this trepanning is connected with pipeline (9) welded seal.Pipeline (9) penetrates and places in the cage (4) with spirrillum.Its effect is the unnecessary heat that carrier top fermentation process is produced, and the mobile water coolant is taken away through pipeline (9) lining, to keep normal leavening temperature.
Three, use said apparatus is produced alcohol with cane molasses technology.This technology and traditional technology are just the same.
Embodiment 2 (production glycerine)
Except using traditional technology with amylum hydrolysate of the sugar fermentative production glycerine instead, remaining is identical with embodiment 1.
Claims (4)
1, a kind of fixing means of viable yeast, it is characterized in that: viable yeast is put into the solubility alginate solution, its strength of solution is the 1-5% weight ratio, stir, so go in the mixed solution of above-mentioned viable yeast and solubility alginate solution, make mixed solution infiltrate the intracavity space of sponge (1) at least one foam sponge (1); Then, take out the sponge (1) of band mixed solution and put into the calcium salt soln that concentration is the solubility of 2%～15% weight ratio, treat sponge (1) internal cavities be settled out be embedded with viable yeast calcium alginate gel (2) afterwards, take out the sponge become viable yeast immobilization carrier (3), clean with clear water.
2, the fixing means of viable yeast according to claim 1 is characterized in that; The solubility alginates is a sodium alginate, and soluble calcium salt is a calcium chloride, and its strength of solution is 2～10% weight ratios.
3, the immobilization viable yeast carrier in the fixing means of viable yeast according to claim 1 and 2 is characterized in that: be filled with embedding in the spumescence network cavity in foam sponge (1) and the calcium alginate gel of viable yeast (2).
4, the application apparatus of the fixing means of viable yeast according to claim 1 and 2, it is characterized in that: a cage (4) that has mesh, immobilization viable yeast carrier (3) is packed in the cage (4), cage (4) is fixing, be installed in fermentor tank housing (5) inner chamber middle part, one feed openings (6) and a carbon dioxide outlet orifice (7) are arranged at housing (5) top, one discharge port (8) is arranged at the bottom, the trepanning on fermentor tank housing (5) wall is passed at the two ends of cooling duct (9) respectively, this trepanning and pipeline (9) are tightly connected, and pipeline (9) penetrates and places in the cage (4) with spirrillum.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95114215A CN1063487C (en) | 1995-10-06 | 1995-10-06 | Live yeast fixing method and device |
| PCT/CN1996/000083 WO1997013850A1 (en) | 1995-10-06 | 1996-10-07 | Living-yeast immobilization method and application device thereof |
| AU72096/96A AU7209696A (en) | 1995-10-06 | 1996-10-07 | Living-yeast immobilization method and application device thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95114215A CN1063487C (en) | 1995-10-06 | 1995-10-06 | Live yeast fixing method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1147553A CN1147553A (en) | 1997-04-16 |
| CN1063487C true CN1063487C (en) | 2001-03-21 |
Family
ID=5080253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95114215A Expired - Fee Related CN1063487C (en) | 1995-10-06 | 1995-10-06 | Live yeast fixing method and device |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN1063487C (en) |
| AU (1) | AU7209696A (en) |
| WO (1) | WO1997013850A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2192986B1 (en) * | 2002-02-25 | 2005-02-01 | Universidad De Cordoba | NEW APPLICATIONS OF GELS CONTAINING FIXED LEAVES. |
| CN100368538C (en) * | 2005-11-14 | 2008-02-13 | 湛江师范学院 | Preparation method of superbranched polymer gel immobilized yeast vector |
| CN106360095A (en) * | 2016-10-13 | 2017-02-01 | 常州市鼎升环保科技有限公司 | Preparation method for feed additive for promoting silkie digestion |
| CN111500386A (en) * | 2020-03-31 | 2020-08-07 | 浙江工商大学 | Immobilized fermentation method for controlling high alcohol content of yellow rice wine |
| CN113073017A (en) * | 2021-04-01 | 2021-07-06 | 浙江大学 | Full-automatic dual-purpose self-brewing intelligent beer machine and method thereof |
| CN112812912A (en) * | 2021-04-01 | 2021-05-18 | 浙江大学 | Household self-separation beer fermentation barrel and fermentation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85105279A (en) * | 1985-04-01 | 1986-09-17 | 四川大学 | Method and T type bio-reactor with producing alcoholic drink with solidified yeast |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064709C (en) * | 1995-04-28 | 2001-04-18 | 广东省微生物研究所 | Yeast fixed carrier and preparation method |
-
1995
- 1995-10-06 CN CN95114215A patent/CN1063487C/en not_active Expired - Fee Related
-
1996
- 1996-10-07 WO PCT/CN1996/000083 patent/WO1997013850A1/en active Application Filing
- 1996-10-07 AU AU72096/96A patent/AU7209696A/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85105279A (en) * | 1985-04-01 | 1986-09-17 | 四川大学 | Method and T type bio-reactor with producing alcoholic drink with solidified yeast |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1147553A (en) | 1997-04-16 |
| WO1997013850A1 (en) | 1997-04-17 |
| AU7209696A (en) | 1997-04-30 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20010321 Termination date: 20101006 |