CN106343541A - Method for preparing collybia albuminosa essence powder through utilizing collybia albuminosa deep fermented mycelia - Google Patents
Method for preparing collybia albuminosa essence powder through utilizing collybia albuminosa deep fermented mycelia Download PDFInfo
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- CN106343541A CN106343541A CN201610718956.4A CN201610718956A CN106343541A CN 106343541 A CN106343541 A CN 106343541A CN 201610718956 A CN201610718956 A CN 201610718956A CN 106343541 A CN106343541 A CN 106343541A
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- termitomycess
- mycelium
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Abstract
The invention relates to a method for preparing collybia albuminosa essence powder through utilizing collybia albuminosa deep fermented mycelia. The method comprises the following steps: (1) deeply fermenting mycelia; (2) crushing the mycelia; (3) carrying out enzymolysis; (4) concentrating; (5) spraying and drying to obtain the collybia albuminosa essence powder. According to the method provided by the invention, a deep fermentation technology is used for cultivating the mycelia, and is combined with an orientated biological enzymolysis technology; the content of peptide-base nitrogen in the obtained collybia albuminosa essence powder reaches 80 percent to 90 percent, and the content of small peptides (with the relative molecular mass being lower than 1000D) is abundant and is 90 percent or more; the collybia albuminosa essence powder has full and mellow umami.
Description
Technical field
The invention belongs to food processing technology field, especially one kind prepare Collybia albuminosa (Berk.) Petch using termitomycess Submerged cultivated mycelium
The method of bacterium fine powder.
Background technology
Termitomycess (termitomyces albuminosus) are under the jurisdiction of Basidiomycetess, Agaricaless, Pleurotaceae, termitomycess
Belong to, be a kind of delicious flavour, nutritious precious wild edible fungus, its not only delicious flavour, and there are some health cares,
There is high Development volue.
There are symbiosiss yet with termitomycess and Coptotermes formosanus Shtrari., sporophore is only capable of growth on termitarium.Additionally, termitarium
On fungus garden, microbe species are various, and the funguses such as Trichoderma spp., penicillium sp, Xylaria sp. fungus, yeast form a fungus " group ", wherein Collybia albuminosa (Berk.) Petch
Though bacterium is dominant microflora, complicated nutrition relationship and ecological setting between termitomycess and Coptotermes formosanus Shtrari., to termitomycess domestication with
Cultivate extreme difficulties, commercialization artificial culture, still in the stage of fumbling, does not cultivate successful report so far both at home and abroad,
Thus limiting its further processing and utilization.
Liquid submerged fermentation technology is one of modern biotechnology, produces mycelium with this technology, can solve termitomycess
The problem of deep processing raw material, and have that with short production cycle, growth conditionss are easy to control, yield is high, produce and be not subject to region and season
Limit, and can be mass-produced, this just provides excellent condition for its exploitation.
By retrieval, not yet find the patent publication us related to present patent application.
Content of the invention
It is an object of the invention in place of overcoming the deficiencies in the prior art, providing one kind to utilize Collybia albuminosa (Berk.) Petch deep-fermentation mycelia
The method that body prepares termitomycess fine powder, the method utilizes submerged fermentation technology cultured mycelia, and overcoming termitomycess can not be complete
Artificial culture and limit the shortcoming of its application, and combine orientation biological enzymolysis technology, the peptidyl nitrogen of the termitomycess fine powder obtaining reaches
To 80-90%, small peptide (relative molecular mass is in below 1000d) rich content, more than 90%, termitomycess fine powder delicate flavour is satisfied
Full, mellow.
The present invention solves its technical problem and takes technical scheme below to realize:
A kind of method preparing termitomycess fine powder using termitomycess Submerged cultivated mycelium, step is as follows:
(1) Submerged cultivated mycelium: with termitomycess as strain, carry out submerged fermentation, mycelial fermentation liquid must be comprised；
(2) mycelium crushes: mycelial fermentation liquid will be comprised using high-speed tissue mashing machine and smash, rotating speed 8000～
12000rpm, time 5～20min, after colloid mill, are homogenized；
(3) digest: adjust ph to 4.5～6.5 using 1mol/l food grade salt acid solution, add mycelium weight 0.01～
0.1% hemicellulase, 45～65 DEG C enzymolysis 1～3h, then with 1mol/l food stage sodium hydroxide solution regulation ph to 7.0～
9.0, add the alkaline protease of mycelium weight 0.01～0.1%, 45～65 DEG C digest 1～3h, more molten with 1mol/l hydrochloric acid
Liquid adjusts ph to 5.5～7.5, adds the flavor protease of mycelium weight 0.01～0.1%, 45～65 DEG C of enzymolysis 1～3h, heats up
To 85～100 DEG C, keep 5～15min, obtain enzymolysis solution；
(4) concentrate: the enzymolysis solution that (3) step is obtained filters, then adopt cryogenic vacuum concentration method to concentrate filtrate, concentrated solution
Quality is 1/2 before concentrating, and thickening temperature is 40～70 DEG C, obtains enzymolysis solution after concentration；
(5) it is spray-dried: after concentrating, enzymolysis solution is spray-dried, and technological parameter is 190 DEG C of inlet temperature, goes out pathogenic wind-warm
85 DEG C of degree, feed liquid flow is 30ml/min, obtains final product termitomycess fine powder.
And, described step concrete operation step (1) is:
1. inoculate: 1cm is taken from mother culture media2Truffle, be transferred under aseptic processing environment through 121 DEG C sterilize
On the slant medium of 30min, it is placed in constant incubator, cultivates 5～15 days for 20～30 DEG C, picking mycelia is healthy and strong, pure white, paving
The strain on full inclined-plane, with 10ml aseptic water elution inclined-plane mycelia, makes mycelium suspension, as liquid seeds；
2. fermentation culture: liquid seeds are poured into fermentation medium, fermentation condition is: inoculum concentration 4～14%, starting ph
6.0～8.5, liquid amount 20～70%, liquid amount refers to account for volume of a container ratio, 20～30 DEG C of cultivation temperature, rotating speed 50～200r/
Min, incubation time 3～10d, obtain final product and comprise mycelial fermentation liquid.
And, described slant medium is: pda culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 20g, water
1000ml, ph are natural；
Described fermentation medium is Semen Maydis powder 10g, wheat bran 10g, glucose 10g, peptone 2g, magnesium sulfate 1g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.2g, vitamin b13mg, water 1000ml.
And, using the filter-cloth filtering of 200 mesh when (4) described step filters.
Advantages of the present invention and good effect are:
1st, the inventive method utilizes submerged fermentation technology cultured mycelia, and combines orientation biological enzymolysis technology, obtains
The peptidyl nitrogen of termitomycess fine powder reaches 80-90%, small peptide (relative molecular mass is in below 1000d) rich content, 90% with
On, termitomycess fine powder delicate flavour is full, mellow.
2nd, the inventive method adopts submerged fermentation technology to obtain termitomycess mycelium, and overcoming termitomycess can not be completely artificial
Cultivation, wild resource is limited, the shortcoming being unfavorable for extensive industrialization, expands processing and the utilization scope of termitomycess.
Specific embodiment
With reference to embodiment, the present invention is further described；Following embodiments are illustrative, are not determinate,
Protection scope of the present invention can not be limited with following embodiments.
Raw material used in the present invention, if no special instructions, is the commercially available prod of routine；Used in the present invention
Method, if no special instructions, is the conventional method of this area.
Embodiment 1:
A kind of method preparing termitomycess fine powder using termitomycess Submerged cultivated mycelium, step is as follows:
(1) Submerged cultivated mycelium: with termitomycess as strain, carry out submerged fermentation, mycelial fermentation liquid must be comprised；
(2) mycelium crushes: mycelial fermentation liquid will be comprised using high-speed tissue mashing machine and smash, rotating speed 8000rpm,
Time 5min, after colloid mill, is homogenized；
(3) digest: adjust ph to 4.5 using 1mol/l food grade salt acid solution, add half fibre of mycelium weight 0.01%
The plain enzyme of dimension, 45 DEG C of enzymolysis 1h, then adjust ph to 7.0 with 1mol/l food stage sodium hydroxide solution, add mycelium weight
0.01% alkaline protease, 45 DEG C of enzymolysis 1h, then adjust ph to 5.5 with 1mol/l hydrochloric acid solution, add mycelium weight
0.01% flavor protease, 45 DEG C of enzymolysis 1h, it is warming up to 85 DEG C, keep 5min, obtain enzymolysis solution；
(4) concentrate: the enzymolysis solution that (3) step is obtained filters, then adopt cryogenic vacuum concentration method to concentrate filtrate, concentrated solution
Quality is 1/2 before concentrating, and thickening temperature is 40 DEG C, obtains enzymolysis solution after concentration；
(5) it is spray-dried: after concentrating, enzymolysis solution is spray-dried, and technological parameter is 190 DEG C of inlet temperature, goes out pathogenic wind-warm
85 DEG C of degree, feed liquid flow is 30ml/min, obtains final product termitomycess fine powder.
Wherein, described step concrete operation step (1) is:
1. inoculate: 1cm is taken from mother culture media2Truffle, be transferred under aseptic processing environment through 121 DEG C sterilize
On the slant medium of 30min, it is placed in constant incubator, cultivate 5 days for 20 DEG C, picking mycelia is healthy and strong, pure white, be paved with inclined-plane
Strain, with 10ml aseptic water elution inclined-plane mycelia, makes mycelium suspension, as liquid seeds；
2. fermentation culture: liquid seeds are poured into fermentation medium, fermentation condition is: inoculum concentration 4%, starting ph 6.0,
Liquid amount 20%, liquid amount refers to account for volume of a container ratio, 20 DEG C of cultivation temperature, rotating speed 50r/min, incubation time 3d, obtains final product bag
Containing mycelial fermentation liquid.
Described slant medium is: pda culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 20g, and water 1000ml, ph are certainly
So；
Described fermentation medium is Semen Maydis powder 10g, wheat bran 10g, glucose 10g, peptone 2g, magnesium sulfate 1g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.2g, vitamin b13mg, water 1000ml.
Embodiment 2:
A kind of method preparing termitomycess fine powder using termitomycess Submerged cultivated mycelium, step is as follows:
(1) Submerged cultivated mycelium: with termitomycess as strain, carry out submerged fermentation, mycelial fermentation liquid must be comprised；
(2) mycelium crushes: mycelial fermentation liquid will be comprised using high-speed tissue mashing machine and smash, rotating speed 10000rpm,
Time 10min, after colloid mill, is homogenized；
(3) digest: adjust ph to 5.5 using 1mol/l food grade salt acid solution, add half fibre of mycelium weight 0.05%
The plain enzyme of dimension, 55 DEG C of enzymolysis 2h, then adjust ph to 8.0 with 1mol/l food stage sodium hydroxide solution, add mycelium weight
0.05% alkaline protease, 55 DEG C of enzymolysis 2h, then adjust ph to 6.5 with 1mol/l hydrochloric acid solution, add mycelium weight
0.05% flavor protease, 55 DEG C of enzymolysis 2h, it is warming up to 90 DEG C, keep 10min, obtain enzymolysis solution；
(4) concentrate: the enzymolysis solution that (3) step is obtained filters, then adopt cryogenic vacuum concentration method to concentrate filtrate, concentrated solution
Quality is 1/2 before concentrating, and thickening temperature is 55 DEG C, obtains enzymolysis solution after concentration；
(5) it is spray-dried: after concentrating, enzymolysis solution is spray-dried, and technological parameter is 190 DEG C of inlet temperature, goes out pathogenic wind-warm
85 DEG C of degree, feed liquid flow is 30ml/min, obtains final product termitomycess fine powder.
Wherein, described step concrete operation step (1) is:
1. inoculate: 1cm is taken from mother culture media2Truffle, be transferred under aseptic processing environment through 121 DEG C sterilize
On the slant medium of 30min, it is placed in constant incubator, cultivate 10 days for 25 DEG C, picking mycelia is healthy and strong, pure white, be paved with inclined-plane
Strain, with 10ml aseptic water elution inclined-plane mycelia, make mycelium suspension, as liquid seeds；
2. fermentation culture: liquid seeds are poured into fermentation medium, fermentation condition is: inoculum concentration 10%, starting ph 7,
Liquid amount 40%, liquid amount refers to account for volume of a container ratio, 25 DEG C of cultivation temperature, rotating speed 100r/min, incubation time 6d, obtains final product bag
Containing mycelial fermentation liquid.
Described slant medium is: pda culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 20g, and water 1000ml, ph are certainly
So；
Described fermentation medium is Semen Maydis powder 10g, wheat bran 10g, glucose 10g, peptone 2g, magnesium sulfate 1g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.2g, vitamin b13mg, water 1000ml.
Using the filter-cloth filtering of 200 mesh when (4) described step filters.
Embodiment 3:
A kind of method preparing termitomycess fine powder using termitomycess Submerged cultivated mycelium, step is as follows:
(1) Submerged cultivated mycelium: with termitomycess as strain, carry out submerged fermentation, mycelial fermentation liquid must be comprised；
(2) mycelium crushes: mycelial fermentation liquid will be comprised using high-speed tissue mashing machine and smash, rotating speed 12000rpm,
Time 20min, after colloid mill, is homogenized；
(3) digest: adjust ph to 6.5 using 1mol/l food grade salt acid solution, add half fibre of mycelium weight 0.1%
The plain enzyme of dimension, 65 DEG C of enzymolysis 3h, then adjust ph to 9.0 with 1mol/l food stage sodium hydroxide solution, add mycelium weight 0.1%
Alkaline protease, 65 DEG C enzymolysis 3h, then with 1mol/l hydrochloric acid solution adjust ph to 7.5, addition mycelium weight 0.1% local flavor
Protease, 65 DEG C of enzymolysis 3h, it is warming up to 100 DEG C, keep 15min, obtain enzymolysis solution；
(4) concentrate: the enzymolysis solution that (3) step is obtained filters, then adopt cryogenic vacuum concentration method to concentrate filtrate, concentrated solution
Quality is 1/2 before concentrating, and thickening temperature is 70 DEG C, obtains enzymolysis solution after concentration；
(5) it is spray-dried: after concentrating, enzymolysis solution is spray-dried, and technological parameter is 190 DEG C of inlet temperature, goes out pathogenic wind-warm
85 DEG C of degree, feed liquid flow is 30ml/min, obtains final product termitomycess fine powder.
Wherein, described step concrete operation step (1) is:
1. inoculate: 1cm is taken from mother culture media2Truffle, be transferred under aseptic processing environment through 121 DEG C sterilize
On the slant medium of 30min, it is placed in constant incubator, cultivate 15 days for 30 DEG C, picking mycelia is healthy and strong, pure white, be paved with inclined-plane
Strain, with 10ml aseptic water elution inclined-plane mycelia, make mycelium suspension, as liquid seeds；
2. fermentation culture: liquid seeds are poured into fermentation medium, fermentation condition is: inoculum concentration 14%, starting ph
8.5, liquid amount 70%, liquid amount refers to account for volume of a container ratio, 30 DEG C of cultivation temperature, rotating speed 200r/min, incubation time 10d,
Obtain final product and comprise mycelial fermentation liquid.
Wherein, described slant medium is: pda culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 20g, water
1000ml, ph are natural；
Described fermentation medium is Semen Maydis powder 10g, wheat bran 10g, glucose 10g, peptone 2g, magnesium sulfate 1g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1.2g, vitamin b13mg, water 1000ml.
Using the filter-cloth filtering of 200 mesh when (4) described step filters.
The inventive method utilizes submerged fermentation technology cultured mycelia, and combines orientation biological enzymolysis technology, the chicken obtaining
The peptidyl nitrogen of fir bacterium fine powder reaches 80-90%, small peptide (relative molecular mass is in below 1000d) rich content, more than 90%,
Termitomycess fine powder delicate flavour is full, mellow.
The above, be only presently preferred embodiments of the present invention, and not technical scheme is made with any form
On restriction.Any simple modification that every technical spirit according to the present invention is made to above example, equivalent variations and repair
Decorations, all still fall within the range of technical scheme.
Claims (4)
1. a kind of using termitomycess Submerged cultivated mycelium prepare termitomycess fine powder method it is characterised in that: step is as follows:
(1) Submerged cultivated mycelium: with termitomycess as strain, carry out submerged fermentation, mycelial fermentation liquid must be comprised；
(2) mycelium crushes: mycelial fermentation liquid will be comprised using high-speed tissue mashing machine and smash, rotating speed 8000～
12000rpm, time 5～20min, after colloid mill, are homogenized；
(3) digest: adjust ph to 4.5～6.5 using 1mol/l food grade salt acid solution, add mycelium weight 0.01～0.1%
Hemicellulase, 45～65 DEG C enzymolysis 1～3h, then with 1mol/l food stage sodium hydroxide solution regulation ph to 7.0～9.0,
Add the alkaline protease of mycelium weight 0.01～0.1%, 45～65 DEG C of enzymolysis 1～3h, then adjusted with 1mol/l hydrochloric acid solution
Ph to 5.5～7.5, adds the flavor protease of mycelium weight 0.01～0.1%, 45～65 DEG C of enzymolysis 1～3h, is warming up to 85
～100 DEG C, keep 5～15min, obtain enzymolysis solution；
(4) concentrate: the enzymolysis solution that (3) step is obtained filters, then adopt cryogenic vacuum concentration method to concentrate filtrate, concentrated solution quality
For 1/2 before concentrating, thickening temperature is 40～70 DEG C, obtains enzymolysis solution after concentration；
(5) it is spray-dried: after concentrating, enzymolysis solution is spray-dried, technological parameter is 190 DEG C of inlet temperature, leaving air temp 85
DEG C, feed liquid flow is 30ml/min, obtains final product termitomycess fine powder.
2. the method preparing termitomycess fine powder using termitomycess Submerged cultivated mycelium according to claim 1, its feature
It is: described step concrete operation step (1) is:
1. inoculate: 1cm is taken from mother culture media2Truffle, be transferred under aseptic processing environment through 121 DEG C of sterilizing 30min
On slant medium, it is placed in constant incubator, cultivate 5～15 days for 20～30 DEG C, picking mycelia is healthy and strong, pure white, be paved with inclined-plane
Strain, with 10ml aseptic water elution inclined-plane mycelia, make mycelium suspension, as liquid seeds；
2. fermentation culture: liquid seeds are poured into fermentation medium, fermentation condition is: inoculum concentration 4～14%, starting ph 6.0
～8.5, liquid amount 20～70%, liquid amount refers to account for volume of a container ratio, 20～30 DEG C of cultivation temperature, rotating speed 50～200r/
Min, incubation time 3～10d, obtain final product and comprise mycelial fermentation liquid.
3. the method preparing termitomycess fine powder using termitomycess Submerged cultivated mycelium according to claim 2, its feature
It is: described slant medium is: pda culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 20g, water 1000ml, ph are natural；
Described fermentation medium is Semen Maydis powder 10g, wheat bran 10g, glucose 10g, peptone 2g, magnesium sulfate 1g, potassium dihydrogen phosphate
1.2g, vitamin b13mg, water 1000ml.
4. the utilization termitomycess Submerged cultivated mycelium according to any one of claims 1 to 3 prepares the side of termitomycess fine powder
Method it is characterised in that: using the filter-cloth filtering of 200 mesh when (4) described step filters.
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| CN104286828A (en) * | 2014-09-24 | 2015-01-21 | 杜超峰 | Tricholoma matsutake vitamin composition, and preparation methods of tricholoma matsutake alcohols and tricholoma matsutake polysaccharides |
| CN105558735A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Edible fungus mycelium fermented drink and making method thereof |
-
2016
- 2016-08-25 CN CN201610718956.4A patent/CN106343541A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455354A (en) * | 2007-12-14 | 2009-06-17 | 中国科学院微生物研究所 | Natural Juncao liver-nourishing and sobering-up agent |
| CN103211212A (en) * | 2013-04-11 | 2013-07-24 | 天津天狮生物发展有限公司 | Cordyceps mycelia and preparation method thereof |
| CN104082037A (en) * | 2014-07-10 | 2014-10-08 | 乳山市华隆生物科技有限公司 | Method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme |
| CN104286828A (en) * | 2014-09-24 | 2015-01-21 | 杜超峰 | Tricholoma matsutake vitamin composition, and preparation methods of tricholoma matsutake alcohols and tricholoma matsutake polysaccharides |
| CN105558735A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Edible fungus mycelium fermented drink and making method thereof |
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