CN106342895A - Antitoxic healing agent prepared from root tubers of dahlia pinnata through excision propagation and use method of antitoxic healing agent - Google Patents
Antitoxic healing agent prepared from root tubers of dahlia pinnata through excision propagation and use method of antitoxic healing agent Download PDFInfo
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- CN106342895A CN106342895A CN201610732454.7A CN201610732454A CN106342895A CN 106342895 A CN106342895 A CN 106342895A CN 201610732454 A CN201610732454 A CN 201610732454A CN 106342895 A CN106342895 A CN 106342895A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention discloses an antitoxic healing agent prepared from root tubers of dahlia pinnata through excision propagation and a use method of the antitoxic healing agent. The antitoxic healing agent is an A-B agent form, wherein the agent A comprises ammonium isobutyrate, 6-benayl aminopurine and 3-indoleacetic acid, the agent B is albefacted wood ash, and a synergistic interaction action is formed between the agent A and the agent B; the weight mass ratio (calculated in 100% of purity) of ammonium isobutyrate to 6-benayl aminopurine to 3-indoleacetic acid in the agent A is (10.0-20.0) to (0.2-0.8) to (6.0-12.0) and is optimally 15.0 to 0.5 to 9.0. The use method comprises steps of diluting the agent A in a concentration of 16.2-32.8 microgram.L<-1>, atomizing incisions of the root tubers, pressing the incisions of the root tubers into the agent B, namely the albefacted wood ash before the incisions are dried, and after the incisions are adhered with the agent B, immediately planting into soil, wherein the agent A is diluted for use when being required, and the operations of preparation and use of the dosage need to be carried out in a shade place. The A-B agent is stable, simple and convenient to operate, remarkable in effect and relatively low in use cost and is a good antitoxic healing agent prepared from the root tubers of the dahlia pinnata through the excision propagation.
Description
Technical field
The invention belongs to agricultural practicality new technique research and development and application, the present invention relates to a kind of Dahlia Pinnata Cav. tuber Excision propagation
The research and development of antitoxin wound curing agent and production application method.
Background technology
Dahlia Pinnata Cav. (dahlia pinnataCav. it is) Compositae, dahlia herbaceos perennial, have huge bar-shaped piece
Root.Stem is upright, multi-branched, and high 1.5-2m is sturdy.Leaf 1-3 returns pinniform and totally cleaves, and upper leaf does not divide sometimes, and sliver is avette or long
Round shape is avette, below celadon, two sides no hair.Head inflorescence is big, has long peduncle, often sagging, wide 6-12cm.Phyllary outer layer
About 5, ovate-elliptic, the food value of leaf, internal layer film quality, ellipticity lanceolar.1 layer of ligulate flower, white, red, or purple, normal ovum
Shape, there are unconspicuous 3 teeth on top, or full edge；Tubular flower yellow, cultigen all ligulate flowers sometimes.Achene Long Circle, long 9-
12mm, wide 3-4 mm, black, flat, there are 2 unconspicuous teeth.The month at florescence 6-12, the fruiting period 9-10 month.Dahlia Pinnata Cav. likes nice and cool
Weather, late September blooms Sheng, but intolerant to frost, after frost, stem and leaf is withered at once.Not tight to temperature requirement in trophophase, 8 ~ 35 DEG C
All can grow, 15 ~ 25 DEG C are advisable.Dahlia Pinnata Cav. is not drought-resistant, intolerant to waterlogging, typically potted plant see that soil is dry and then pour permeable, accomplish to see and wet see
Dry.
Dahlia Pinnata Cav. originates in Mexico, and at present, world's majority state is all planted, and being that whole world cultivation is the widest views and admires plant
Thing, 20 beginnings of the century introduced China, and all there is cultivation multiple provinces and regions now.According to statistics, Dahlia Pinnata Cav. kind, more than 30,000, is the world
One of most species of upper flower variety.Dahlia Pinnata Cav. pattern flower-shape reputation name is various, rich and varied, is one of world's famous flower；Beautiful greatly
Hua Xingxin, sweet, flat, there are heat-clearing and toxic substances removing, promoting blood circulation to remove blood stasis, there is certain medical value.
It is big that the propagation method of Dahlia Pinnata Cav. has root division, cutting propagation, seminal propagation etc., root division and cutting propagation
The main method of beautiful flower breeding.Root division is the Excision propagation of tuber: between the month in spring 3-4, take out the tuber of storage, will be every
One tuber and the bud be born on root neck of attachment cut down simultaneously, separately plant.Because Dahlia Pinnata Cav. only root cervical region just has bud, so
It is necessary to there be the root neck with bud on each tuber of segmentation；On unrooted neck or root neck, the tuber of no bud point does not all sprout it is impossible to plant
Plant.If on root neck germinate point inconspicuous or be difficult identification, accelerating germination can be shifted to an earlier date in early spring, in hotbed by network of roots with closeer away from
From sequencing, then hilling, water, give certain temperature, divide again after sprouting, each plant division at least has a bud.Plant division
Breeding is simple and easy to do, and survival rate is high, robust plant, but its otch easily infects the diseases such as Dahlia Pinnata Cav. mosaic viruss.
The mosaic virus of Dahlia Pinnata Cav. are popular the widest, and morbidity is the heaviest.Mainly pass through during root division, tuber cutting to be drawn
Caused by playing infection, next to that the band poison of the insect such as aphid and leafhopper is propagated.Generate on the incidence of leaf of Dahlia Pinnata Cav. mosaic virus
Pattern-like decorative pattern, at vein and its nearby becomes light green color, sick leaf development harmful effect is bloomed, and plant shrinkage when serious becomes short,
Have a strong impact on its ornamental value.Particularly in morbidity epidemic-stricken area, occur even more serious, aggrieved serious infection rate is up to more than 50%.
Conventional control method: from nontoxic propagating materialss, reduce source of infection；Nontoxic Seedling is bred using detoxification and tissue culture method, and
Note in time controlling passing poisonous insect matchmaker after planting；Note rural area health, reduce virus infection source, spray 50% Malathion
1000 times of liquid, 1500 times of liquid of 40% Rogor or 800 times of liquid of 25% sevin.The above prevention and controls are all effective, but mainly
It is to propagate with poison for the cultivation of nontoxic Seedling and insect, do not have specifically because tuber cuts the infection causing to during root division
Effective measures.
The antitoxin wound curing agent of this Dahlia Pinnata Cav. tuber Excision propagation efficiently solves this difficult problem, and it is not only to during root division
The infection that tuber cutting causes has significant antitoxic action moreover it is possible to promote the wound wound healing as early as possible that tuber stays because of cutting, raw
Root germinates.This agent is easy to use, simple to operate, saves labor, low cost, antitoxin wound healing effect is significant, is that Dahlia Pinnata Cav. produces solution
Determine this difficult problem.
Content of the invention
The purpose of the present invention aims to provide a kind of antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation, and this antitoxin wound curing agent makes
With convenient, simple to operate, low cost, antitoxin wound healing effect is significant.Another object of the present invention aims to provide above-mentioned Dahlia Pinnata Cav. block
The preferably using method of the antitoxin wound curing agent of root Excision propagation.The purpose of the present invention is achieved in the following ways:
The antitoxin wound curing agent of the present invention is made up of ab agent it may be assumed that a agent is by isopropylformic acid. ammonium+6-benzyl aminopurine+3-indolyl acetic acid group
Become, b agent is albefaction bavin ash, ab agent can play good synergistic function.This agent, b agent are packed with plastic bag sealing respectively, a
Agent is placed in cold preservation in refrigerator, and the b agent place of drying disposed within deposits, and can preserve 1-2.This agent produces upper easy to use, operation letter
Single, low cost, antitoxin wound healing effect is significant, the shelf-life is longer, is packed with plastic bag sealing, can carry out factorial praluction, is formed
Product.
The preparation method of the antitoxin wound curing agent of this Dahlia Pinnata Cav. tuber Excision propagation is:
A agent: isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are 10.0～20.0:0.2
～0.8:6.0～12.0, optimum weight mass ratio is 15.0:0.5:9.0, and three components all press 100% purity using dissolvable dosage forms
Weight quality meter, on the premise of not impact effect, its content height is not subject to for other implants outside effective ingredient or impurity
Limit.Packed with plastic bag sealing, cold preservation in refrigerator, 1-2 can be preserved.
B agent: sieve more than albefaction bavin ash 100 mesh, remove coarse granule, packed with plastic bag sealing, normal temperature laboratory is dried
Place deposits, and can preserve 1-2.
The antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation of the present invention has no the report of like product at present, and the present invention is to send out
The result of the test that a person of good sense is drawn through experimental study for many years and production practices, and tested in the production of long period
Card, effect is preferable.And the formula of the present invention also has that technology content is higher, dosage form is more stable, abundant raw material source, operation letter
Just, commodity are such as developed into, for producing Dahlia Pinnata Cav., low price.Easy to use aborning, antitoxin wound healing effect shows
Write, use cost is relatively low, is a kind of good antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation.This agent prescription can carry out batch production
Produce, its exploitation not only will commercially increase a kind of antitoxin wound curing agent of new Dahlia Pinnata Cav. tuber Excision propagation, will be also
The problem of Dahlia Pinnata Cav. tuber Excision propagation infection pathogen provides a new solution route at present.
The present invention is prepared into ab dosage form from isopropylformic acid. ammonium+6-benzyl aminopurine+3-indolyl acetic acid and albefaction bavin ash, adopts
The compatibility of Suitable weight mass ratio, is configured to the antitoxin wound curing agent of commercial Dahlia Pinnata Cav. tuber Excision propagation, and it has and significantly resists
Malicious wound healing effect.The following is the comparative test of its antitoxin wound healing effect.
The using method of the present invention is:
Between the month in spring 3-4, take out the tuber of storage, the bud that each tuber and attachment are born on root neck cuts down simultaneously.Make
Used time isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid mass ratio by weight are put into warm concussion in a small amount of ethanol and are dissolved, and three
16.2～32.8 g.l are pressed with water again after component dissolving-1Concentration dilution, optimum diluted concentration be 24.5 g.l-1.After dilution i.e.
Can use, be used while allocating.Operation will be carried out in lucifuge, shady place.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed
Mist, it is important that incision necessarily will be sprayed onto position.After slightly dry receipts water, before that is, incision is not dry, its otch is pressed into b agent, makes
Albefaction bavin ash is all stained with otch, then can plant and bury.Planting buries selects at cloudy day or fine day to carry out the dusk time-division.
The following is inventor and realize the purpose of the present invention, constantly pass through test and select and big from the suitable present invention
The comparison examination of the antitoxin wound healing effect that the antitoxin raw material of wound healing agent prescription of beautiful colored tuber Excision propagation and its proportioning of correlation are done
Test.
Single group separating tests
Single group separating tests are mainly qualitative and quantitative and test each component to the antitoxin, wound healing of Dahlia Pinnata Cav. tuber Excision propagation and root
The one-component effect test that system occurs.
Materials and methods
1.1.1 test material
Test is from the mosaic virus more universal to the harm of Dahlia Pinnata Cav. tuber Excision propagation, incision wound and root system situation occurred
As evaluation index.Test single dose is: isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin four components of ash.
Wherein isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid belong to the component of a agent, and albefaction bavin ash belongs to b agent component.
Test method
Test is carried out in the epidemic-stricken area of Dahlia Pinnata Cav. mosaic virus.Spring by the end of March, takes out the tuber of storage, by each tuber and attached
The bud being born on root neck cuts down simultaneously.Operation will be carried out in lucifuge, shady place.
Isopropylformic acid. ammonium presses 15.0 g.l with water-1Concentration dilution (based on the weight quality of 100% purity), after dilution at once
Use, be used while allocating.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will be sprayed onto
Position, then plants and buries.Planting buries selects to carry out in of fine day the dusk time-division.
After 6-benzyl aminopurine puts into warm concussion dissolving in a small amount of ethanol, then press 0.5 g.l with water-1Concentration dilution
(based on the weight quality of 100% purity), uses after dilution at once, is used while allocating.With aerosol apparatus, Dahlia Pinnata Cav. Excision propagation is cut
Mouth is sprayed, it is important that incision necessarily will be sprayed onto position, then plants and buries.Plant and bury selection in the dusk of fine day
Divide and carry out.
3-indolyl acetic acid puts in a small amount of ethanol warm concussion dissolving, after press 9.0 g.l with water again-1Concentration dilution (press
The weight quality meter of 100% purity), use after dilution at once, be used while allocating.With the otch to Dahlia Pinnata Cav. Excision propagation for the aerosol apparatus
Sprayed, it is important that incision necessarily will be sprayed onto position, then plant and bury.Planting in fine day for the selection of burying the dusk time-division
Carry out.
Albefaction bavin ash checks whether humidity, from dry albefaction bavin ash, opens and can use after packing, prevents from inhaling
Tide.Before the otch of Dahlia Pinnata Cav. Excision propagation is not dry, its otch press-in albefaction bavin ash, make all to be stained with albefaction bavin on otch
Ash, then plants and buries.Planting buries selects to carry out in of fine day the dusk time-division.
Spray 1500 times of liquid of 40% Rogor every 7d after emerging, propagated with the band poison preventing the insects such as aphid and leafhopper.Test
Effect is mainly festered with the decline percentage ratio of its mosaic virus, otch and is declined percentage ratio, root system generation quantity rising percentage
Than counting and statistical analysiss.After 30d observed and recorded otch fester, root system occur quantity effect, observed and recorded floral leaf after 60d
The sickness rate of virosiss.Test setting 4 processes 1 comparison, and each processes 3 and repeats (including ck), respectively processes random distributed points.
Each numbering that processes is to process 1-4 and ck.Each disposition is as follows:
Process 1 isopropylformic acid. ammonium water by 15.0 g.l-1Concentration dilution (based on the weight quality of 100% purity), dilution
Use afterwards at once, be used while allocating.Operation is carried out in lucifuge, shady place.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is carried out
Spraying, it is important that incision necessarily will be sprayed onto position, then plants and buries.Planting buries selects to enter in of fine day the dusk time-division
OK.30th, observed and recorded test effect after 60d.Process 2 6-benzyl aminopurines and put into warm concussion dissolving in a small amount of ethanol
Afterwards, then with water press 0.5 g.l-1Concentration dilution (based on the weight quality of 100% purity), use after dilution at once, with join with
With.Operation is carried out in lucifuge, shady place.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision
Necessarily position will be sprayed onto, then plant and bury.Planting buries selects to carry out in of fine day the dusk time-division.30th, observed and recorded after 60d
Test effect.Process after 3 3-indolyl acetic acids put in a small amount of ethanol warm concussion dissolving, then press 9.0 g.l with water-1Dense
Degree dilution (based on the weight quality of 100% purity), uses after dilution at once, is used while allocating.Operation is carried out in lucifuge, shady place.
With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will be sprayed onto position, then plant into
Soil.Planting buries selects to carry out in of fine day the dusk time-division.30th, observed and recorded test effect after 60d.Process 4 albefaction bavins
Ash checks whether humidity, from dry albefaction bavin ash, opens and can use after packing, prevent the moisture absorption.Operation is in lucifuge, the moon
Liang Chu is carried out.At once among its otch press-in albefaction bavin ash after the cutting of Dahlia Pinnata Cav. tuber, with cutting with pressure, make all viscous on otch
Upper albefaction bavin ash, then plants and buries.Planting buries selects to carry out in of fine day the dusk time-division.30th, observed and recorded test after 60d
Effect.Comparison (ck) is left intact in the otch of Dahlia Pinnata Cav. Excision propagation, directly plants and buries.30th, observe after 60d
Record test effect.
Statistical analysiss
Sas(6.12 is adopted to test data) carry out statistical analysiss.Result of the test shows (table 1), processes the mosaic virus of 1-4
Rate of descent is noticeably greater than ck, and the mosaic virus rate of descent processing 4 is noticeably greater than other process, is secondly process 1, process 3, place
Reason 2.The mosaic virus rate of descent that i.e. isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin ash are processed is noticeably greater than
Ck, the mosaic virus rate of descent of albefaction bavin ash is noticeably greater than other process, is secondly to process isopropylformic acid. ammonium, process 3- indole second
Acid and process 6-benzyl aminopurine.
Table 1. single group separating tests (unit: %)
| Process | Mosaic virus rate of descent | Otch festers rate of descent | There is quantity climbing in root system |
| Process 1 | 34.79±2.49b | 21.42±0.94d | 11.07±1.57c |
| Process 2 | 18.26±1.73d | 25.83±3.72c | 3.49±0.39d |
| Process 3 | 26.72±0.82c | 31.60±1.69b | 27.31±2.85a |
| Process 4 | 42.86±4.17a | 38.27±2.81a | 17.64±1.47b |
| ck | 2.61±0.35e | 1.84±0.42e | 3.91±0.37d |
Note: lower case is 0.05 level, same row difference letter representation significant difference, similarly hereinafter.
The otch rate of descent that festers processing 1-4 is noticeably greater than ck, and the otch rate of descent that festers processing 4 is noticeably greater than its elsewhere
Reason, is secondly process 3, process 2, processes 1.I.e. process isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin ash
The otch rate of descent that festers is noticeably greater than ck, and the otch of the albefaction bavin ash rate of descent that festers is noticeably greater than other process, is secondly to process
3-indolyl acetic acid, process 6-benzyl aminopurine and process isopropylformic acid. ammonium.
The root system processing 1-4 occurs quantity climbing to be noticeably greater than ck, and the root system processing 3 occurs quantity climbing significantly big
In other process, secondly it is process 4, processes 1, process 2.Process isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, white
The root system changing bavin ash occurs quantity climbing to be noticeably greater than ck, and the root system of 3-indolyl acetic acid occurs quantity climbing to be noticeably greater than it
He is processed, and is secondly to process albefaction bavin ash, process isopropylformic acid. ammonium and process 6-benzyl aminopurine.
Shown by above single group separating tests, isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, the flower of albefaction bavin ash
Fester rate of descent, root system of leaf disease viral disease rate of descent, otch occurs quantity climbing to be significantly higher than ck, processes the flower of albefaction bavin ash
Leaf disease viral disease rate of descent, otch fester rate of descent be significantly higher than other process, process 3-indolyl acetic acid root system occur quantity
Climbing is significantly higher than other process.
Compatibility mechanism
Compatibility mechanism is mainly qualitative and quantitative and tests each ingredients to the antitoxin, wound healing of Dahlia Pinnata Cav. tuber Excision propagation and root
The composite effect test that system occurs.
Materials and methods
2.1.1 test material
Test is from the mosaic virus more universal to the harm of Dahlia Pinnata Cav. tuber Excision propagation, incision wound and root system situation occurred
As evaluation index.Test ingredients is: isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin four components of ash.
Wherein isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid belong to a agent, and albefaction bavin ash belongs to b agent.
Test method
The antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation of the present invention is made up of ab agent it may be assumed that a agent is by isopropylformic acid. ammonium+6- benzyl amino
Purine+3-indolyl acetic acid composition, b agent is made up of albefaction bavin ash.This agent, b agent are packed with plastic bag sealing respectively, and a agent is placed in ice
Cold preservation in case, the b agent place of drying disposed within deposits, and preserves 2 years.
A agent: isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are 10.0～20.0:
0.2～0.8:6.0～12.0(tri- component all using dissolvable dosage forms based on the weight quality of 100% purity, outside effective ingredient
, on the premise of not impact effect, its content height is unrestricted for other implants or impurity).Packed with plastic bag sealing, ice
Cold preservation in case, preserves 2 years.
B agent: sieve more than albefaction bavin ash 100 mesh, remove coarse granule, packed with plastic bag sealing, normal temperature laboratory is dried
Place deposits, and preserves 2 years.
Spring by the end of March, takes out the tuber of storage, and the bud that each tuber and attachment are born on root neck cuts down simultaneously.
Operation will be carried out in lucifuge, shady place.1500 times of liquid of 40% Rogor are sprayed every 7d, to prevent the evil such as aphid and leafhopper after emerging
The band poison of worm is propagated.Test is carried out in the epidemic-stricken area in Dahlia Pinnata Cav. mosaic virus former years.Test effect mainly uses its mosaic virus
Fester decline percentage ratio, root system of the decline percentage ratio of disease, otch occurs quantity to rise percentage ratio to count and statistical analysiss.30d
Afterwards observed and recorded otch fester, root system occur quantity effect, the sickness rate of observed and recorded mosaic virus after 60d.Test sets
Put 3 and process 1 comparison, each processes 3 and repeats (including ck), respectively processes random distributed points.Each process numbering be process 1-3 with
ck.Each disposition is as follows:
Processing 1 a agent by the weight mass ratio of isopropylformic acid. ammonium, 6-benzyl aminopurine, three components of 3-indolyl acetic acid is 10.0:
0.2:6.0, after putting into warm concussion dissolving in a small amount of ethanol, then presses 24.5 g.l with water-1Concentration dilution (by 100% purity
Weight quality meter), use after dilution at once, be used while allocating.Operation is carried out in lucifuge, shady place.With aerosol apparatus, Dahlia Pinnata Cav. is cut
The otch cutting breeding is sprayed, it is important that incision necessarily will be sprayed onto position.Then, before otch is not dry, its otch
Among press-in b agent albefaction bavin ash, make albefaction bavin ash is all stained with otch, plant at once and bury.Plant and bury selection in fine day
Carried out the dusk time-division.30th, observed and recorded test effect after 60d.Process 2 a agent by isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-
The weight mass ratio of three components of heteroauxing is 15.0:0.5:9.0, after putting into warm concussion dissolving in a small amount of ethanol, then uses
Water presses 24.5 g.l-1Concentration dilution (based on the weight quality of 100% purity), use after dilution at once, be used while allocating.Operation
Carry out in lucifuge, shady place.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will
It is sprayed onto position.Then, before otch is not dry, among its otch press-in b agent albefaction bavin ash, make all to be stained with albefaction bavin on otch
Ash, plants at once and buries.Planting buries selects to carry out in of fine day the dusk time-division.30th, observed and recorded test effect after 60d.Place
Managing 3 a agent by the weight mass ratio of isopropylformic acid. ammonium, 6-benzyl aminopurine, three components of 3-indolyl acetic acid is 20.0:0.8:
12.0, after putting into warm concussion dissolving in a small amount of ethanol, then press 24.5 g.l with water-1The concentration dilution (weight by 100% purity
Amount quality meter), use after dilution at once, be used while allocating.Operation is carried out in lucifuge, shady place.With aerosol apparatus, Dahlia Pinnata Cav. is cut
The otch of breeding is sprayed, it is important that incision necessarily will be sprayed onto position.Then, before otch is not dry, its otch pressure
Enter among b agent albefaction bavin ash, make albefaction bavin ash is all stained with otch, plant at once and bury.Plant selection being close in fine day of burying
The late time-division is carried out.30th, observed and recorded test effect after 60d.Comparison (ck) is not appointed in the otch of Dahlia Pinnata Cav. Excision propagation
Where is managed, and directly plants and buries.30th, observed and recorded test effect after 60d.
Statistical analysiss
Sas(6.12 is adopted to test data) carry out statistical analysiss.Result of the test shows (table 2), processes the mosaic virus of 1-3
Rate of descent is noticeably greater than ck, and the mosaic virus rate of descent processing 2 is noticeably greater than other process, is secondly process 1,3.I.e. a agent
It is 10.0～20.0:0.2～0.8 by the weight mass ratio of isopropylformic acid. ammonium, 6-benzyl aminopurine, three components of 3-indolyl acetic acid:
After the otch of 6.0～12.0 pairs of Dahlia Pinnata Cav. Excision propagations is sprayed, then the mosaic viruss its otch being stained with b agent albefaction bavin ash
Sick rate of descent is noticeably greater than ck, and the weight mass ratio of three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid is pressed in a agent
Mosaic virus rate of descent for 15.0:0.5:9.0 is noticeably greater than other process, secondly presses isopropylformic acid. ammonium, 6- benzyl amino for a agent
Purine, the weight mass ratio of three components of 3-indolyl acetic acid are the process of 10.0:0.2:6.0 and 20.0:0.8:12.0.
Table 2. compatibility mechanism (unit: %)
| Process | Mosaic virus rate of descent | Otch festers rate of descent | There is quantity climbing in root system |
| Process 1 | 89.37±3.47b | 81.29±3.94c | 91.64±2.64ab |
| Process 2 | 95.24±2.18a | 97.28±4.75a | 93.25±1.79a |
| Process 3 | 91.30±2.47b | 86.09±2.49b | 89.46±3.52b |
| ck | 2.61±0.37c | 1.84±0.49d | 3.91±0.31c |
The otch rate of descent that festers processing 1-3 is noticeably greater than ck, and the otch rate of descent that festers processing 2 is noticeably greater than other process,
Secondly it is process 3, again for processing 1.I.e. the weight of three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid is pressed in a agent
Mass ratio be 10.0～20.0:0.2～0.8:6.0～12.0 pair Dahlia Pinnata Cav. Excision propagation otch spray after, then it
The otch rate of descent that festers that otch is stained with b agent albefaction bavin ash is noticeably greater than ck, and isopropylformic acid. ammonium, 6-benzyl aminopurine, 3- Yin are pressed in a agent
Secondly the weight mass ratio of three components of indolylbutyric acid is that the otch rate of descent that festers of 15.0:0.5:9.0 is noticeably greater than other process,
The weight mass ratio pressing three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid for a agent is 20.0:0.8:12.0's
Process, the process for 10.0:0.2:6.0 again.
The root system processing 1-3 occurs quantity climbing to be noticeably greater than ck, and the root system processing 2 occurs quantity climbing and process
1 no significant difference, but noticeably greater than process 3.I.e. three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid are pressed in a agent
Weight mass ratio is that the root system of 10.0～20.0:0.2～0.8:6.0～12.0 occurs quantity climbing to be noticeably greater than ck, and a agent is pressed
Isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are cut to Dahlia Pinnata Cav. for 15.0:0.5:9.0
Cut breeding otch sprayed after, then its otch be stained with b agent albefaction bavin ash root system occur quantity climbing with a agent by different
Butanoic acid ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are that the process of 10.0:0.2:6.0 is no notable
Difference, but noticeably greater than a agent by the weight mass ratio of isopropylformic acid. ammonium, 6-benzyl aminopurine, three components of 3-indolyl acetic acid is
20.0:0.8:12 process.
Above test shows, the weight quality of three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid is pressed in a agent
After spraying than the otch for 10.0～20.0:0.2～0.8:6.0～12.0 pair Dahlia Pinnata Cav. Excision propagation, then its otch
Be stained with b agent albefaction bavin ash mosaic virus rate of descent, otch fester rate of descent, root system occur quantity climbing be noticeably greater than
Ck, wherein presses the weight mass ratio of three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid as 15.0:0.5 with a agent:
After the otch of 9.0 pairs of Dahlia Pinnata Cav. Excision propagations is sprayed, then it is best that its otch is stained with the grey effect of b agent albefaction bavin.
Test it is also shown that the weight matter of three components of isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid is pressed in a agent above
After amount is sprayed than the otch for 10.0～20.0:0.2～0.8:6.0～12.0 pair Dahlia Pinnata Cav. Excision propagation, then it is cut
Mouth be stained with b agent albefaction bavin ash mosaic virus rate of descent, otch fester rate of descent, root system occur quantity climbing (table 2) show
Write more than isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin ash four one pack systems mosaic virus rate of descent,
Otch fester rate of descent, root system occur quantity climbing (table 1), the effect (table 2) of ab agent is each one pack system effect (table 1)
One to several times.It is indicated above that isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin ash four component compatibilities have relatively
Good synergistic function.
Dosetest
Dosetest is mainly qualitative and quantitative and tests antitoxin, wound healing to Dahlia Pinnata Cav. tuber Excision propagation for each using dosage
The composite effect test occurring with root system.
Test material
Test is from the mosaic virus more universal to the harm of Dahlia Pinnata Cav. tuber Excision propagation, incision wound and root system situation occurred
As evaluation index.Test ingredients is: isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid, albefaction bavin four components of ash.
Wherein isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-indolyl acetic acid belong to a agent, and albefaction bavin ash belongs to b agent.
Test method
The antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation of the present invention is made up of ab agent it may be assumed that a agent is by isopropylformic acid. ammonium+6- benzyl amino
Purine+3-indolyl acetic acid composition, b agent is albefaction bavin ash.This agent, b agent are packed with plastic bag sealing respectively, and a agent is placed in refrigerator
Cold preservation, the b agent place of drying disposed within deposits, and preserves 2 years.
A agent: isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are 15.0:0.5:
9.0(tri- component all using dissolvable dosage forms based on the weight quality of 100% purity, other implants outside effective ingredient or impurity
On the premise of not impact effect, its content height is unrestricted).Packed with plastic bag sealing, cold preservation in refrigerator, preserve 2 years.
B agent: sieve more than albefaction bavin ash 100 mesh, remove coarse granule, packed with plastic bag sealing, normal temperature laboratory is dried
Place deposits, and preserves 2 years.
Spring by the end of March, takes out the tuber of storage, and the bud that each tuber and attachment are born on root neck cuts down simultaneously.
Operation will be carried out in lucifuge, shady place.1500 times of liquid of 40% Rogor are sprayed every 7d, to prevent the evil such as aphid and leafhopper after emerging
The band poison of worm is propagated.Test is carried out in the epidemic-stricken area in Dahlia Pinnata Cav. mosaic virus former years.Test effect mainly uses its mosaic virus
Fester decline percentage ratio, root system of the decline percentage ratio of disease, otch occurs quantity to rise percentage ratio to count and statistical analysiss.30d
Afterwards observed and recorded otch fester, root system occur quantity effect, the sickness rate of observed and recorded mosaic virus after 60d.Test sets
Put 3 and process 1 comparison, each processes 3 and repeats (including ck), respectively processes random distributed points.Each process numbering be process 1-3 with
ck.Each disposition is as follows:
Processing 1 a agent by the weight mass ratio of isopropylformic acid. ammonium, 6-benzyl aminopurine, three components of 3-indolyl acetic acid is 15.0:
0.5:9.0, after putting into warm concussion dissolving in a small amount of ethanol, then presses 16.2 g.l with water-1Concentration dilution (by 100% purity
Weight quality meter), use after dilution at once, be used while allocating.Operation is carried out in lucifuge, shady place.With aerosol apparatus, Dahlia Pinnata Cav. is cut
The otch cutting breeding is sprayed, it is important that incision necessarily will be sprayed onto position.Then, before otch is not dry, its otch
Among press-in b agent albefaction bavin ash, make albefaction bavin ash is all stained with otch, plant at once and bury.Plant and bury selection in fine day
Carried out the dusk time-division.30th, observed and recorded test effect after 60d.Process 2 a agent by isopropylformic acid. ammonium, 6-benzyl aminopurine, 3-
The weight mass ratio of three components of heteroauxing is 15.0:0.5:9.0, after putting into warm concussion dissolving in a small amount of ethanol, then uses
Water presses 24.5 g.l-1Concentration dilution (based on the weight quality of 100% purity), use after dilution at once, be used while allocating.Operation
Carry out in lucifuge, shady place.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will
It is sprayed onto position.Then, before otch is not dry, among its otch press-in b agent albefaction bavin ash, make all to be stained with albefaction bavin on otch
Ash, plants at once and buries.Planting buries selects to carry out in of fine day the dusk time-division.30th, observed and recorded test effect after 60d.Place
Managing 3 a agent by the weight mass ratio of isopropylformic acid. ammonium, 6-benzyl aminopurine, three components of 3-indolyl acetic acid is 15.0:0.5:
9.0, after putting into warm concussion dissolving in a small amount of ethanol, then press 32.8 g.l with water-1The concentration dilution (weight by 100% purity
Quality meter), use after dilution at once, be used while allocating.Operation is carried out in lucifuge, shady place.With aerosol apparatus, Dahlia Pinnata Cav. is cut numerous
The otch grown is sprayed, it is important that incision necessarily will be sprayed onto position.Then, before otch is not dry, its otch is pressed into b
Among agent albefaction bavin ash, make albefaction bavin ash is all stained with otch, plant at once and bury.Plant and bury selection in the dusk of fine day
Divide and carry out.30th, observed and recorded test effect after 60d.Comparison (ck) does not do any place in the otch of Dahlia Pinnata Cav. Excision propagation
Reason, directly plants and buries.30th, observed and recorded test effect after 60d.
Statistical analysiss
Sas(6.12 is adopted to test data) carry out statistical analysiss.Result of the test shows (table 3), processes the mosaic virus of 1-3
Rate of descent is noticeably greater than ck, and the mosaic virus rate of descent processing 2 is noticeably greater than other process, is secondly process 1,3.I.e. a agent
By 16.2-32.8 g.l-1Concentration the otch of Dahlia Pinnata Cav. Excision propagation is sprayed after, then its otch is stained with b agent
The mosaic virus rate of descent of albefaction bavin ash is noticeably greater than ck, and 24.5 g.l are pressed in a agent-1Concentration mosaic virus decline
Rate is noticeably greater than other process, secondly presses 16.2 g.l for a agent-1With 32.8 g.l-1Concentration process.
Table 3. dosetest (unit: %)
| Process | Mosaic virus rate of descent | Otch festers rate of descent | There is quantity climbing in root system |
| Process 1 | 92.41±1.37b | 94.30±2.64ab | 92.07±1.27a |
| Process 2 | 95.24±2.04a | 97.28±4.16a | 93.25±1.49a |
| Process 3 | 91.27±0.93b | 91.48±1.80b | 87.26±0.71b |
| ck | 2.61±0.34c | 1.84±0.43c | 3.91±0.29c |
The otch rate of descent that festers processing 1-3 is noticeably greater than ck, and the otch rate of descent that festers processing 2 is noticeably greater than process 3, but with
Process 1 no significant difference.I.e. 16.2-32.8 g.l is pressed in a agent-1Concentration the otch of Dahlia Pinnata Cav. Excision propagation is sprayed
Afterwards, then its otch the otch rate of descent that festers being stained with b agent albefaction bavin ash is noticeably greater than ck, and 24.5 g.l are pressed in a agent-1Using dense
The otch of degree festers rate of descent noticeably greater than a agent by 32.8 g.l-1Concentration process, but with a agent press 16.2 g.l-1
Concentration process no significant difference.
The root system processing 1-3 occurs quantity climbing to be noticeably greater than ck, and the root system processing 1-2 occurs quantity climbing notable
More than process 3, process 1 and process 2 no significant difference.I.e. 16.2-32.8 g.l is pressed in a agent-1Concentration to Dahlia Pinnata Cav. cut
After the otch of breeding is sprayed, then the root system that its otch is stained with b agent albefaction bavin ash occurs quantity climbing to be noticeably greater than ck,
16.2-24.5 g.l is pressed in a agent-1Concentration root system occur quantity climbing be noticeably greater than a agent press 32.8 g.l-1Make
With the process of concentration, but 16.2 g.l are pressed in a agent-1Concentration and a agent press 24.5 g.l-1Concentration process no aobvious
Write difference.
Above test shows, 16.2-32.8 g.l is pressed in a agent-1Concentration the otch of Dahlia Pinnata Cav. Excision propagation is carried out
After spraying, then its otch be stained with b agent albefaction bavin ash mosaic virus rate of descent, otch fester rate of descent, root system occur number
The effect is significant of amount climbing.Wherein, 24.5 g.l are pressed in a agent-1Concentration the otch of Dahlia Pinnata Cav. Excision propagation is sprayed
After mist, then its otch be stained with b agent albefaction bavin ash mosaic virus rate of descent, otch fester rate of descent, root system occur quantity
The effect of climbing is best.
Test it is also shown that the effect (table 2-3) of ab agent is significantly higher than the effect (table of each one pack system of ab agent above
1), its effect is one to the several times of each one pack system effect.It is indicated above that each one pack system compatibility of ab agent becomes ab agent to have relatively
Good synergistic function.
Isopropylformic acid. ammonium has sterilization, promotes the physiological action of cellular metabolism.6-benzyl aminopurine can promote calluss
Formed, promote the division of bud primordial cell, the division of suppression root restriction cell.3-indolyl acetic acid has the division promoting cell,
Promote calli induction, promote the division of root restriction cell, to form lateral root, adventitious root.Albefaction bavin ash alkalescence, in otch
Place forms alkaline environment, is unfavorable for growth and the breeding of microorganism, has bactericidal action；It also contains abundant trace element,
If many trace element such as mg are important cofactor or its ingredients of cellular metabolism holoenzyme, improve or to have activated some important
Activity of enzyme reaction, stimulate and promote the metabolism of incision cell, be conducive to calli induction etc. to act on.
Specific embodiment
Implementation below and embodiment are intended to further illustrate the present invention, rather than limitation of the present invention.
Embodiment 1:
From the antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation, this agent is a, b dosage form it may be assumed that a agent is by isopropylformic acid. ammonium+6- benzyl amino
Purine+3-indolyl acetic acid composition, b agent is made up of albefaction bavin ash.This agent, b agent are packed with plastic bag sealing respectively, and a agent is placed in ice
Cold preservation in case, the b agent place of drying disposed within deposits.
A agent: isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are 15.0:0.5:
9.0(tri- component all using dissolvable dosage forms based on the weight quality of 100% purity, other implants outside effective ingredient or impurity
On the premise of not impact effect, its content height is unrestricted).Packed with plastic bag sealing, cold preservation in refrigerator, effect duration 2
Year.
B agent: sieve more than albefaction bavin ash 100 mesh, remove coarse granule, packed with plastic bag sealing, normal temperature laboratory is dried
Place deposits, and can preserve 2 years.
Spring by the end of March, takes out the tuber of storage, and the bud that each tuber and attachment are born on root neck cuts down simultaneously.
Operation will be carried out in lucifuge, shady place.A agent is put into after warm concussion dissolving in a small amount of ethanol, then presses 16.2 g.l with water-1's
Concentration dilution (based on the weight quality of 100% purity), uses after dilution at once, is used while allocating.Operation is entered in lucifuge, shady place
OK.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will be sprayed onto position.Then, exist
Before otch is not dry, among its otch press-in b agent albefaction bavin ash, makes albefaction bavin ash is all stained with otch, plant thereafter at once
Bury.Planting buries selects to carry out in of fine day the dusk time-division.After 30d observed and recorded otch fester, root system occur quantity effect
Fruit be: otch fester rate of descent be 94.30%, root system occur quantity climbing be 92.07%.Observed and recorded mosaic viruss after 60d
The effect of disease is: mosaic virus rate of descent is 92.41%.
Embodiment 2:
From the antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation, this agent is a, b dosage form it may be assumed that a agent is by isopropylformic acid. ammonium+6- benzyl amino
Purine+3-indolyl acetic acid composition, b agent is made up of albefaction bavin ash.This agent, b agent are packed with plastic bag sealing respectively, and a agent is placed in ice
Cold preservation in case, the b agent place of drying disposed within deposits.
A agent: isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are 15.0:0.5:
9.0(tri- component all using dissolvable dosage forms based on the weight quality of 100% purity, other implants outside effective ingredient or impurity
On the premise of not impact effect, its content height is unrestricted).Packed with plastic bag sealing, cold preservation in refrigerator, effect duration 2
Year.
B agent: sieve more than albefaction bavin ash 100 mesh, remove coarse granule, packed with plastic bag sealing, normal temperature laboratory is dried
Place deposits, and can preserve 2 years.
Spring by the end of March, takes out the tuber of storage, and the bud that each tuber and attachment are born on root neck cuts down simultaneously.
Operation will be carried out in lucifuge, shady place.A agent is put into after warm concussion dissolving in a small amount of ethanol, then presses 24.5 g.l with water-1's
Concentration dilution (based on the weight quality of 100% purity), uses after dilution at once, is used while allocating.Operation is entered in lucifuge, shady place
OK.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will be sprayed onto position.Then, exist
Before otch is not dry, among its otch press-in b agent albefaction bavin ash, makes albefaction bavin ash is all stained with otch, plant thereafter at once
Bury.Planting buries selects to carry out in of fine day the dusk time-division.After 30d observed and recorded otch fester, root system occur quantity effect
Fruit be: otch fester rate of descent be 97.28%, root system occur quantity climbing be 93.25%.Observed and recorded mosaic viruss after 60d
The effect of disease is: mosaic virus rate of descent is 95.24%.
Embodiment 3:
From the antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation, this agent is a, b dosage form it may be assumed that a agent is by isopropylformic acid. ammonium+6- benzyl amino
Purine+3-indolyl acetic acid composition, b agent is made up of albefaction bavin ash.This agent, b agent are packed with plastic bag sealing respectively, and a agent is placed in ice
Cold preservation in case, the b agent place of drying disposed within deposits.
A agent: isopropylformic acid. ammonium, 6-benzyl aminopurine, the weight mass ratio of three components of 3-indolyl acetic acid are 15.0:0.5:
9.0(tri- component all using dissolvable dosage forms based on the weight quality of 100% purity, other implants outside effective ingredient or impurity
On the premise of not impact effect, its content height is unrestricted).Packed with plastic bag sealing, cold preservation in refrigerator, effect duration 2
Year.
B agent: sieve more than albefaction bavin ash 100 mesh, remove coarse granule, packed with plastic bag sealing, normal temperature laboratory is dried
Place deposits, and can preserve 2 years.
Spring by the end of March, takes out the tuber of storage, and the bud that each tuber and attachment are born on root neck cuts down simultaneously.
Operation will be carried out in lucifuge, shady place.A agent is put into after warm concussion dissolving in a small amount of ethanol, then presses 32.8 g.l with water-1's
Concentration dilution (based on the weight quality of 100% purity), uses after dilution at once, is used while allocating.Operation is entered in lucifuge, shady place
OK.With aerosol apparatus, the otch of Dahlia Pinnata Cav. Excision propagation is sprayed, it is important that incision necessarily will be sprayed onto position.Then, exist
Before otch is not dry, among its otch press-in b agent albefaction bavin ash, makes albefaction bavin ash is all stained with otch, plant thereafter at once
Bury.Planting buries selects to carry out in of fine day the dusk time-division.After 30d observed and recorded otch fester, root system occur quantity effect
Fruit be: otch fester rate of descent be 91.48%, root system occur quantity climbing be 87.26%.Observed and recorded mosaic viruss after 60d
The effect of disease is: mosaic virus rate of descent is 91.27%.
Claims (5)
1. a kind of antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation and its using method are it is characterised in that described Dahlia Pinnata Cav. block
The antitoxin wound curing agent of root Excision propagation is ab dosage form, and a agent is made up of isopropylformic acid. ammonium, 6-benzyl aminopurine and 3-indolyl acetic acid, b agent
For albefaction bavin ash.
2. a kind of antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation according to claim 1 is it is characterised in that its a agent
Isopropylformic acid. ammonium, 6-benzyl aminopurine and 3-indolyl acetic acid three component weight mass ratio be 10.0～20.0:0.2～0.8:
6.0～12.0, the weight mass ratio that three components press 100% purity calculates.
3. a kind of antitoxin wound curing agent of the Dahlia Pinnata Cav. tuber Excision propagation according to claim 1 and 2 is it is characterised in that its a
The preferred ratio of the isopropylformic acid. ammonium of agent, 6-benzyl aminopurine and 3-indolyl acetic acid three component is 15.0:0.5:9.0, and three components are pressed
The weight mass ratio of 100% purity calculates.
4. the Dahlia Pinnata Cav. tuber Excision propagation according to claim 2 to 3 antitoxin wound curing agent it is characterised in that use when
First 16.2～32.8 g.l are pressed in a agent-1Concentration dilution after tuber otch is sprayed, then, before otch is not dry,
So as to after otch is all stained with b agent, plant and bury at once among its otch press-in b agent albefaction bavin ash.
5. the antitoxin wound curing agent of Dahlia Pinnata Cav. tuber Excision propagation according to claim 4 using method it is characterised in that
The dilution of a agent will be used while allocating, the preparation of medicament and use, and its operation will be carried out in lucifuge shady place.
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