CN106336946A - Supercritical carbon dioxide sealwort seed oil extraction method - Google Patents
Supercritical carbon dioxide sealwort seed oil extraction method Download PDFInfo
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- CN106336946A CN106336946A CN201610926228.2A CN201610926228A CN106336946A CN 106336946 A CN106336946 A CN 106336946A CN 201610926228 A CN201610926228 A CN 201610926228A CN 106336946 A CN106336946 A CN 106336946A
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- separating still
- extraction
- rhizoma polygonati
- temperature
- carbon dioxide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
Abstract
The invention relates to a supercritical carbon dioxide sealwort seed oil extraction method which comprises pretreatment, charging, heating process, pressure boosting process, analytical calculation and other processes. Sealwort seeds are smashed and put into an extraction kettle of a supercritical CO2 extraction device, the temperatures and pressures of the extraction kettle and a separating kettle are respectively set, and sealwort seed fat oil is collected from the separating kettle and the yield of the oil is calculated after the extraction time is reached. The supercritical carbon dioxide sealwort seed oil extraction method adopts supercritical carbon dioxide to extract the sealwort seeds, does not need an organic solvent and does not include follow-up treatment, the extraction time is short, the extraction efficiency is high, unit time yield is high, a fat oil sample is superior than a traditional method in clarity, color and luster, and no solvent is left.
Description
Technical field
The present invention relates to plant seed abstraction technique field, particularly to a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed
The method of oil.
Background technology
Rhizoma Polygonati is Liliaceae Polygonatum Medicinal Plants, herbaceos perennial, and there are more than 60 kinds in the whole world, is distributed mainly on north temperature
Band and north subtropical, China has 31 kinds, is distributed widely in except southern extratropical wide geographic area." Chinese Pharmacopoeia " version in 2015
Rhizoma Polygonati polygonatum sibiricum red., Polygonatum cyrtonema Hua p.cyrtonema hua and P. kingianum are recorded
The dry rhizome of p.kingianum coll.et hemsl. is as Rhizoma Polygonati medical material.Rhizoma Polygonati is traditional conventional Chinese medicine of China, begins
Be loaded in Shanxi generation " Mingyi Bielu ", be classified as top grade, afterwards the successive dynasties medicine ancient books and records all on the books to it, its mild in medicine property and, sweet in the mouth easily take,
There is boosting qi and nourishing yin, spleen invigorating lung moistening, kidney tonifying promote the production of body fluid, blood pressure lowering, blood sugar lowering, blood fat reducing and the effects such as slow down aging.
At present the research of Rhizoma Polygonati is focused mostly on and analyze in main medicinal part pharmacological research and chemical constituent, emerging in recent years
The research and development of Rhizoma Polygonati functional food and health product are played.Research with regard to Rhizoma Polygonati seed and its chemical composition, especially its
Liposoluble constituent, hitherto reported is less.Rhizoma Polygonati seed production is big, and averagely every plant of ripe plant can produce 200 about seeds, many
Person, up to 400, is the new resources being worth exploitation.
Traditional solvent extraction method extracts fatty oil, mostly adopts continuous circumfluence extraction method, but extraction time is long, Er Qie
Use substantial amounts of organic reagent during extraction, extract easy times of organic reagent pollution of the fatty oil product obtaining, exist certain
The dissolvent residual of degree.
Content of the invention
Greatly and organic easy in order to solve Rhizoma Polygonati seed extraction time length in above-mentioned technical background, consumption of organic solvent
It is difficult to reclaim, causes the problems such as environmental pollution and dissolvent residual, the present invention proposes a kind of supercritical carbon dioxide extraction Rhizoma Polygonati kind
The method of seed oil.
The technical scheme is that and be achieved in that, a kind of side of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil
Method, comprises the following steps:
(1) pretreatment: it is 24-100 mesh that Rhizoma Polygonati seed is crushed to particle diameter, standby；
(2) feed: weigh the Rhizoma Polygonati seed 100-200g after step (1) is pulverized, put into supercritical co2The extraction of extraction equipment
Take in kettle, tighten extraction kettle cover, and check separation kettle cover and have whether closing valve is closed；
(3) temperature-rise period: startup power supply, connect cooling water, start cold-producing medium switch, and open extraction kettle, separating still
Heater switch, and set the temperature of extraction kettle as 40-60 DEG C, the temperature of separating still is 40-60 DEG C；
(4) boost process: be down to 5-6 DEG C in refrigerator temps, and the temperature of extraction kettle, separating still reaches the temperature of setting
And when stablizing, open co2Gas cylinder and air-source inflow valve, first discharge extract and separate system in air, work as extracting pressure
When consistent with pressure of storage tank, start co2Pump, makes extraction kettle pressure be gradually increased to 26-38mpa, adjusts the regulating valve of separating still, point
It is 6-19mpa from kettle pressure, and extraction time is set for 30-180min；
(5) analytical calculation: after reaching extraction time, collect Rhizoma Polygonati seed fat oil from separating still, by gas phase color
Spectrum GC-MS is tested and analyzed, and calculates its yield, and yield computing formula is: yield=Rhizoma Polygonati seed fat oil total amount
(g)/Rhizoma Polygonati seed dosage (g) × 100%.
Preferably, the method for described a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, in described step (1)
The particle diameter of Rhizoma Polygonati seed is 60-80 mesh.
Preferably, the method for described a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, in described step (2)
Medical material injected volume is 150g.
Preferably, a kind of described supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil method it is characterised in that: institute
State separating still in step (3) and include separating still, separating still and separating still, the temperature of separating still is 40-60 DEG C, separating still
Temperature be 50-60 DEG C, the temperature of separating still is 40-60 DEG C.
More excellent, a kind of method of described supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil it is characterised in that: institute
The temperature stating separating still is 60 DEG C, and the temperature of separating still is 50 DEG C, and the temperature of separating still is 40 DEG C, the temperature of extraction kettle
For 40 DEG C.
Preferably, the method for described a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, in described step (3)
Adjust the regulating valve of separating still, separating still pressure is 11-19mpa, separating still pressure is 6-10mpa, separating still pressure is
6-8mpa.
More excellent, a kind of oily method of described supercritical carbon dioxide extraction Rhizoma Polygonati seed fat, in described step (3)
Adjust the regulating valve of separating still, separating still pressure is 19mpa, separating still pressure is 6mpa, separating still pressure is 6mpa.
Preferably, the method for described a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, in described step (4)
co2Flow be: 6-20kg/ (h kg medical material), co2The frequency of pump is 25-40hz.
More excellent, a kind of oily method of described supercritical carbon dioxide extraction Rhizoma Polygonati seed fat, in described step (4)
co2Flow be: 13.5kg/ (h kg medical material), co2The frequency of pump is 31hz.
Preferably, the method for described a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, in described step (4)
Extraction time is 90min.
The invention has the beneficial effects as follows: the present invention adopts supercritical carbon dioxide extraction Rhizoma Polygonati seed, without using organic
, there is not subsequent treatment in solvent, and extraction time is short, and extraction efficiency is high, unit interval high income, and fatty oil samples are clear and bright
Degree, color and luster are superior to traditional method, no solvent residue.
Brief description
Fig. 1 is the gas chromatogram of the Rhizoma Polygonati seed oil extracting in the embodiment of the present invention 3；
Fig. 2 is the gas chromatogram of the Rhizoma Polygonati seed oil that ether extraction extracts in the embodiment of the present invention 4；
Fig. 3 is the gas chromatogram of the Rhizoma Polygonati seed oil that the embodiment of the present invention 4 petrochina ether extraction method is extracted.
Specific embodiment
For more fully understanding the present invention, below by following examples, the present invention is made specifically to illustrate further, but not
Can be regarded as limitation of the invention, some made according to foregoing invention content for those skilled in the art are nonessential
Improve and adjustment, be also considered as being within the scope of the present invention.
Embodiment 1
A kind of method of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, comprises the following steps:
(1) pretreatment: it is 24-100 mesh that Rhizoma Polygonati seed is crushed to particle diameter, standby；
(2) feed: weigh the Rhizoma Polygonati seed 100-200g after step (1) is pulverized, put into supercritical co2The extraction of extraction equipment
Take in kettle, tighten extraction kettle cover, and check separation kettle cover and have whether closing valve is closed；
(3) temperature-rise period: startup power supply, connect cooling water, start cold-producing medium switch, and open extraction kettle, separating still,
Separating still, the heater switch of separating still；And setting the temperature of extraction kettle as 40-60 DEG C, the temperature of separating still is 40-60
DEG C, the temperature of separating still is 50-60 DEG C, and the temperature of separating still is 40-60 DEG C；
(4) boost process: be down to 5-6 DEG C in refrigerator temps, and the temperature of extraction kettle, separating still reaches the temperature of setting
And when stablizing, open co2Gas cylinder and air-source inflow valve, first discharge extract and separate system in air, work as extracting pressure
When consistent with pressure of storage tank, start co2Pump, makes extraction kettle pressure be gradually increased to 26-38mpa, adjusts the regulating valve of separating still, point
It is 11-19mpa from kettle pressure, separating still pressure is 6-10mpa, separating still pressure is 6-8mpa, starts the cycle over extraction,
co2Flow be: 6-20kg/ (h kg medical material), co2The frequency of pump is 25-40hz, and extraction time is 30-180min；
(5) analytical calculation: after reaching extraction time, gleanings from separating still, separating still, separating still respectively
Material, obtains Rhizoma Polygonati seed fat oil, by gas chromatograph-mass spectrometer (trace1300-isq, U.S. thermo fisher
Scientific company) tested and analyzed, because Rhizoma Polygonati seed fat oil base originally concentrates on separating still, so finally to separate
The fatty oil of kettle is target product calculated yield.
Described supercritical co2Extraction equipment, Zhongshan University's Chinese medicine Integrated manufacture and process control technology research center and south
Logical sagacity supercritical technology ＆ development Co. combines manufacture, and model 231-50-06, including 1 extraction kettle, separating still, separating still
, separating still.
Embodiment 2
Impact supercritical co2The factor of extraction efficiency has a lot, and wherein the impact to supercritical extraction for the pressure and temperature is
Greatly.The principal element of determination impact extraction is extracting pressure, extraction temperature, separating still pressure, separating still temperature (keep dividing
From the pressure force-summing device loop of kettle is consistent, 40 DEG C of temperature, extract 90min), and adopt l9 (3) 4 Orthogonal Experiment and Design, with Huang
Smart seed fat oil yield and outward appearance (shade, whether clear and bright) are evaluation index, on the basis of embodiment 1, carry out excellent
Change the test of extractant condition, orthogonal test is referring to table 1.
Knowable to the range analysiss of table 1, four factors to the impact size of Rhizoma Polygonati seed oil extraction yield are: separating still pressure
Power > separating still temperature > extracting pressure > extraction temperature；According to the mean analysis of table 1, the maximum under four influence factors
Average is respectively as follows: separating still pressure 3.094, separating still temperature 2.316, extraction temperature 2.242, extracting pressure 2.372, institute
With supercritical co2In the optimised process combinatorial theory of extraction should be: a3b1c3d1；But due between three levels of pressure factor
Extreme difference is only 0.304, and the extreme difference between three levels of extraction temperature factor is only 0.223, three levels of separating still temperature factor
Between extreme difference be only 0.365, illustrate in this pressure limit, in extraction temperature, in separating still separation temperature, three kinds of factors pair
The impact very little of yield, it is contemplated that the energy consumption issues of actual production, finally determines supercritical co2The optimised process combination of extraction
For: a1b1c3d1, i.e. extracting pressure and respectively 26mpa and 40 DEG C of temperature, separating still pressure and temperature is respectively 19mpa and 60
DEG C, separating still pressure and temperature temperature is respectively 6mpa and 50 DEG C, and separating still pressure and temperature is respectively 6mpa and 40 DEG C,
Extraction time is 90min.
Table 1 orthogonal design table
Embodiment 3
A kind of method of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil, comprises the following steps:
(1) pretreatment: it is 60-80 mesh that Rhizoma Polygonati seed is crushed to particle diameter, standby；
(2) feed: weigh the Rhizoma Polygonati seed 150g after step (1) is pulverized, put into supercritical co2Extraction equipment (ibid)
In extraction kettle, tighten extraction kettle cover, and check separation kettle cover and have whether closing valve is closed；
(3) temperature-rise period: startup power supply, connect cooling water, start cold-producing medium switch, and open extraction kettle, separating still,
Separating still, the heater switch of separating still；And setting the temperature of extraction kettle as 40 DEG C, the temperature of separating still is 60 DEG C, separates
The temperature of kettle is 50 DEG C, and the temperature of separating still is 40 DEG C；
(4) boost process: be down to 5-6 DEG C in refrigerator temps, and the temperature of extraction kettle, separating still reaches the temperature of setting
And when stablizing, open co2Gas cylinder and air-source inflow valve, first discharge extract and separate system in air, work as extracting pressure
When consistent with pressure of storage tank, start co2Pump, makes extraction kettle pressure be gradually increased to 26mpa, adjusts the regulating valve of separating still, separate
Kettle pressure is 19mpa, and separating still pressure is 6mpa, and separating still pressure is 6mpa, starts the cycle over extraction, co2Flow is:
13.5kg/ (h kg medical material), co2The frequency of pump is 31hz, and extraction time is 90min；
(5) analytical calculation: after reaching extraction time, gleanings from separating still, separating still, separating still respectively
Material, is obtained Rhizoma Polygonati seed fat oil, is tested and analyzed by gas chromatograph-mass spectrometer, because of Rhizoma Polygonati seed fat oil base originally
Concentrate on separating still, so finally with the fatty oil of separating still for target product calculated yield.
Embodiment 4
Ether extraction
List of references (Li Yonggang, Ji Jingyu, Zhang Yuejin. the mensure of Rhizoma Polygonati seed nutritional composition and analysis [j]. northwest is planted
Thing journal, 2009,29 (8): 1692-1696；Ge Fahuan edits. and Chinese medicine supercritical carbon dioxide extraction method studies [m]. north
Capital: China Medical Science Press, 2014) and appropriately adjust, Rhizoma Polygonati seed will be dried, and (water content meets " Chinese Pharmacopoeia "
2015 editions standards) pulverize, accurately weigh 5g powder and extracted.Extraction solvent is absolute ether, temperature 50 C, time 12h.Carry
After the completion of taking, boil off absolute ether, be cooled to room temperature, weigh, calculated yield.
Petroleum ether extraction method
List of references (Ge Fahuan edits. and Chinese medicine supercritical carbon dioxide extraction method studies [m]. Beijing: Chinese Medicine section
Skill publishing house, 2014) and appropriately adjust, Rhizoma Polygonati seed (water content meets " Chinese Pharmacopoeia " the 2015 editions standards) powder that will be dried
Broken.Accurately weigh 100g powder, add 8 times amount petroleum ether (boiling point is 60-90 DEG C), 80 DEG C of heating in water bath backflow 12h.Extract
Cheng Hou, sucking filtration, filtrate steaming petroleum ether, it is cooled to room temperature, weigh, calculated yield.
The fatty oil that obtain ether extraction, Petroleum ether extraction method and embodiment 3 supercritical carbon dioxide extraction obtain
Rhizoma Polygonati seed fat oil be analyzed comparing, result is as shown in table 2.
The Rhizoma Polygonati seed fat oil results contrast that table 2 Different Extraction Method obtains
As shown in Table 2, compared with ether extraction, Petroleum ether extraction method, supercritical co2The extraction used time is short, during unit
Between high income, and fatty oil samples clarity, color and luster are superior to ether extraction and Petroleum ether extraction method, and solvent-free residual
Stay.
Meanwhile, 25mg ether extraction, Petroleum ether extraction method, and the Rhizoma Polygonati seed fat that embodiment 1 is extracted are taken respectively
Oil, in 150ml conical flask, adds 10ml normal hexane, is allowed to fully dissolve；Add the koh-ch of 10ml, 0.5mol/l3Oh, room
The lower shake well 4min of temperature, room temperature stands 30min, shakes 6min after the 10ml that adds water, and takes the supernatant, adds anhydrous sodium sulfate,
By 0.45 μm of filtering with microporous membrane, obtain into sample liquid.Sample liquid will be entered it will be injected into gas chromatograph-mass spectrometer (ibid) to carry out
Gc-ms analyzes, and its analysis condition is as follows:
GC conditions: 250 DEG C of injection port, the high-pure helium of carrier gas 99.999%, carrier gas flux 1.5ml/min, chromatograph
Post tr-5ms (10m × 25m × 0.25 μm).Temperature programming is 180 DEG C and rises, and rises to 200 DEG C with 8 DEG C/min, retains 5min, with 2
DEG C/min rises to 230 DEG C, retains 5min, rises to 280 DEG C with 5 DEG C/min, retains 5min.
Mass Spectrometry Conditions: solvent delay 5min；250 DEG C of injector temperature, split ratio 15:1, carrier gas is helium, flow velocity
1.5ml/min, sample size: 1 μ l.280 DEG C of transfer tube temperature；Ei ion source, 230 DEG C of ion source temperature；Ei ion source, electronic energy
Amount 70ev；Full scan, sweep limitss: m/z25-500u.
By above-mentioned gc-ms condition to ether extraction, Petroleum ether extraction method, and supercritical co2The fatty oil that method is extracted
Sample is analyzed respectively, the gas chromatogram spectrogram of the Analysis of Fatty Acids Composition of Rhizoma Polygonati seed oil obtaining respectively as shown in Figure 1, Figure 2,
Shown in Fig. 3.
Fatty acid component content results in the Rhizoma Polygonati seed fat oil that table 3 Different Extraction Method obtains
The mass spectrum of each component is retrieved through computer standard spectrum storehouse nist11, in conjunction with literature survey and artificial spectrogram solution
Analysis, according to areas of peak normalization method, judges fatty acid component and its content of Rhizoma Polygonati seed fat oil, as shown in table 3.
Supercritical co2Main fatty acid component in the fatty oil that extraction and ether extraction, Petroleum ether extraction method obtain
Basically identical, it is all stearic acid, Palmic acid, Oleic acid, linoleic acid, simply ether extraction, Petroleum ether extraction method are than supercritical co2
Extraction some trace fatty acid compositions many, but supercritical co2Unsaturated fatty acid in extraction Rhizoma Polygonati seed fat oil
Content (71.57%) be higher than traditional method (ether extraction 66.27%, Petroleum ether extraction method 65.21%).
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of method of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil is it is characterised in that comprise the following steps:
(1) pretreatment: it is 24-100 mesh that Rhizoma Polygonati seed is crushed to particle diameter, standby；
(2) feed: weigh the Rhizoma Polygonati seed 100-200g after step (1) is pulverized, put into supercritical co2The extraction kettle of extraction equipment
In, tighten extraction kettle cover, and check separation kettle cover and have whether closing valve is closed；
(3) temperature-rise period: startup power supply, connect cooling water, start cold-producing medium switch, and open the heating of extraction kettle, separating still
Switch, and set the temperature of extraction kettle as 40-60 DEG C, the temperature of separating still is 40-60 DEG C；
(4) boost process: be down to 5-6 DEG C in refrigerator temps, and the temperature of extraction kettle, separating still reach setting temperature and
When stablizing, open co2Gas cylinder and air-source inflow valve, first discharge extract and separate system in air, when extracting pressure and storage
When pressure tank is consistent, start co2Pump, makes extraction kettle pressure be gradually increased to 26-38mpa, adjusts the regulating valve of separating still, separating still
Pressure is 6-19mpa, and arranges extraction time for 30-180min；
(5) analytical calculation: after reaching extraction time, collect Rhizoma Polygonati seed fat oil from separating still, by gas chromatogram matter
Spectrum combined instrument is tested and analyzed, and calculates its yield, and yield computing formula is: yield=Rhizoma Polygonati seed fat oil total amount (g)/
Rhizoma Polygonati seed dosage (g) × 100%.
2. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 1, its feature exists
In: in described step (1), the particle diameter of Rhizoma Polygonati seed is 60-80 mesh.
3. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 1, its feature exists
In: described step (2) Chinese crude drug injected volume is 150g.
4. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 1, its feature exists
In: in described step (3), separating still includes separating still, separating still and separating still, and the temperature of separating still is 40-60 DEG C,
The temperature of separating still is 50-60 DEG C, and the temperature of separating still is 40-60 DEG C.
5. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 4, its feature exists
In: the temperature of described separating still is 60 DEG C, and the temperature of separating still is 50 DEG C, and the temperature of separating still is 40 DEG C, extraction kettle
Temperature is 40 DEG C.
6. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 4, its feature exists
In: adjust the regulating valve of separating still in described step (3), separating still pressure is 11-19mpa, separating still pressure is 6-
10mpa, separating still pressure is 6-8mpa.
7. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 4, its feature exists
In: adjust the regulating valve of separating still in described step (3), separating still pressure is 19mpa, separating still pressure is 6mpa, separates
Kettle pressure is 6mpa.
8. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 1, its feature exists
In: co in described step (4)2Flow be: 6-20kg/ (h kg medical material), co2The frequency of pump is 25-40hz.
9. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 1, its feature exists
In: co in described step (4)2Flow be: 13.5kg/ (h kg medical material), co2The frequency of pump is 31hz.
10. the method for a kind of supercritical carbon dioxide extraction Rhizoma Polygonati seed fat oil according to claim 1, its feature exists
In: in described step (4), extraction time is 90min.
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| CN107281273A (en) * | 2017-07-24 | 2017-10-24 | 广东药科大学 | A kind of method of supercritical carbon dioxide extracting fruit of glossy privet liposoluble constituent |
| CN107384584A (en) * | 2017-07-25 | 2017-11-24 | 广东药科大学 | A kind of method of supercritical carbon dioxide extracting fruit of glossy privet lubricant component |
| CN107418722A (en) * | 2017-05-18 | 2017-12-01 | 毕节市中药产业发展办公室 | Use supercritical CO2Extract Rosa roxburghii seed oil |
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| CN104073343A (en) * | 2014-07-15 | 2014-10-01 | 中山大学 | Supercritical CO2 aquilaria agallocha roxb seed oil extraction method |
| CN104694245A (en) * | 2013-12-09 | 2015-06-10 | 孟庆海 | A method for obtaining Chinese wolfberry seed oil by supercritical carbon dioxide extraction |
| CN105018219A (en) * | 2015-06-29 | 2015-11-04 | 广西大学 | Method for supercritical CO2 extraction of coix seed oil |
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| CN102295988A (en) * | 2011-07-24 | 2011-12-28 | 江苏江大源生态生物科技有限公司 | Supercritical carbon dioxide extraction method of gardenia whole fruit oil |
| CN104694245A (en) * | 2013-12-09 | 2015-06-10 | 孟庆海 | A method for obtaining Chinese wolfberry seed oil by supercritical carbon dioxide extraction |
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| CN107418722A (en) * | 2017-05-18 | 2017-12-01 | 毕节市中药产业发展办公室 | Use supercritical CO2Extract Rosa roxburghii seed oil |
| CN107281273A (en) * | 2017-07-24 | 2017-10-24 | 广东药科大学 | A kind of method of supercritical carbon dioxide extracting fruit of glossy privet liposoluble constituent |
| CN107384584A (en) * | 2017-07-25 | 2017-11-24 | 广东药科大学 | A kind of method of supercritical carbon dioxide extracting fruit of glossy privet lubricant component |
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Application publication date: 20170118 |