CN106333959B - Protective effect of the hydrogen-rich water to premature ovarian failure - Google Patents
Protective effect of the hydrogen-rich water to premature ovarian failure Download PDFInfo
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- CN106333959B CN106333959B CN201510418601.9A CN201510418601A CN106333959B CN 106333959 B CN106333959 B CN 106333959B CN 201510418601 A CN201510418601 A CN 201510418601A CN 106333959 B CN106333959 B CN 106333959B
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Abstract
The present invention is a kind of method of anti-premature ovarian failure.This method is to enable the daily drinking hydrogen-rich water of mouse, and mouse is made freely to intake feed.Method of the invention can be such that the ovarian function of premature ovarian failure mouse and reactivity is improved, and reduce the quantity of apoptosis granular cell in ovary, therefore be of great significance to the premature ovarian failure for resisting female mice.In the mankind, there is amenorrhoea in premature ovarian failure before being described as 40 years old, and Gonadotropin Level increases (such as FSH > 40IU/L) in blood.The complicated multiplicity of its cause of disease, however at present still without inventing the treatment method that the disease final result can be reversed.Once there is premature ovarian failure, the termination of reproductive function will imply that.It currently, many studies have reported the protective effects of hydrogen-rich water tracts a variety of for whole body, however is still blind area for female reproductive system, therefore scholar's exploration developing is badly in need of in the field.Very safe in view of hydrogen-rich water, without side-effects and mankind's female repro ductive system and mouse similitude, and the crowd of premature ovarian failure there is no effective treatment method, which can be used for improving the course of disease of human immunity premature ovarian failure.
Description
Technical field
The present invention relates to the methods that premature ovarian failure is treated and prevented with hydrogen-rich water, belong to biology and new medicine field.
Background technique
Primary product of the active oxygen as oxidative stress can influence egg mother cell, embryo itself and its locating microenvironment.
Aerobic metabolism can stimulating activity oxygen generate, i.e. prooxidant, and what restricted activity oxygen generated is antioxidant, both this is once
The unbalance generation that just will lead to oxidative stress.In genital tract, active oxygen plays effect of both physiology and pathology, adjusts
The signal of interest of female reproductive system physiological function, participation oocyte maturation, ovarian follicle generate, endometrial cycle sexually revises,
Steroid hormone synthesis maintains the physiology courses such as luteal function, luteolysis, however, excessive active oxygen but will be to system genitale
Each function of system generates certain damage, such as the damage or deformity even infertility of ovum.In addition, the generation pair of free radical
Has the function of double-edged sword in female reproductive system, especially for ovulation process.
Infertility is defined as: the sexual intercourse 1 year under without any safeguard measure and fail to become pregnant.Although infertility disease incidence
Increase year by year, but since the cause of disease is not fully understood, even if whole issue can not be solved using tube-test baby techniques.At present
It has proven convenient that oxidative stress will impact with supplementary reproduction fertility naturally, excessive lipid peroxidation be women not
One of pregnant physiological pathology factor.The past oxidation resistance of studies have shown that idiopathic infertile patient is substantially reduced, and oxidative stress
The lipid peroxidation injury of induction but obviously increases.
In the ovary of mammal, the development of granular cell and egg mother cell is closely related.In primordial follicle and ovarian follicle
In formation, granular cell is not only limited to ovarian cumulus, or forms the main component of ovarian follicle wall.Granular cell can be solid with secretory class
Alcohol hormone, growth factor, cell factor etc., this is most important to the growth and maintenance of ovarian follicle.In addition, granular cell may be used also
To adjust the selection of final preovulatory follicle.Oxidative stress can be with the apoptosis of induced particle cell.T-2 toxin can pass through oxidation
Stress pathways dose-dependently inhibit the growth of granular cell in rat ovary, induce its apoptosis, this mainly passes through activity
What the mitochondria pathway that oxygen mediates was completed.In addition, low tempertaure storage induction oxidative stress can also by activation caspase-8,
The apoptosis pathway of caspase-9, caspase-3 dependence mediates the apoptosis of granular cell.In addition, oxidative stress is also to ovarian follicle
Developing direction plays conclusive effect.The growth of antral follicle count can be inhibited by having studies have shown that active oxygen not only, can also be started
The apoptosis of antral follicle count.
Hydrogen is a kind of safely and effectively antioxidant, it can penetrate biomembrane, and disperse enters endochylema, mitochondria and thin
In karyon, the hydroxyl radical free radical (i.e. most cytotoxic free radical) of corresponding site is eliminated by selectivity, protects cell
With tissue from the damage of oxidative stress.In addition to this, hydrogen also has Anti-G value.Hydrogen can produce a variety of diseases
Raw therapeutic oxidation resistant effect, but for the protective effect of premature ovarian failure not yet studies have reported that.
The study find that the generation of mouse immune premature ovarian failure can be resisted by the method for drinking hydrogen-rich water, improve
The concentration of catalase in Mouse Ovary Tissues improves the function and reactivity of premature ovarian failure mouse ovarian, reduces mouse eggs
The ratio of nest apoptosis of granulosa cell, so that the reproductive system function to premature ovarian failure mouse plays certain protective effect.By
There is certain similitude in the reproductive system of the mankind and the reproductive system of mouse, while its safety of hydrogen-rich water postscript is without side-effects,
Therefore, hydrogen-rich water can be used as the function that drug improves infertile women ovary, while can preventative protection ovary tissue appearance
Early ageing.
Summary of the invention
The present invention is a kind of method for fighting premature ovarian failure by drinking hydrogen-rich water.Using female in test
BALB/c mouse is divided into 4 groups.Model group and mould hydrogen group mouse prepare premature ovarian failure model；Blank group and hydrogen water
Group mouse does not prepare premature ovarian failure model；Blank group and model group mouse persistently drink light water；Hydrogen water group and mould hydrogen
Group mouse persistently drinks hydrogen-rich water.The change for carrying out the vaginal smear observation oestrous cycle to mouse daily, until model group mouse
There is the disorder (35 days or so from modeling first day) of oestrous cycle, whole mouse are put to death to the detection for carrying out index of correlation.
Result of study shows that a kind of concentration of hydrogen peroxide enzyme (antioxidase) can be changed and improved by persistently drinking hydrogen-rich water, improves
The concentration of Anti-Mullerian hormone AMH in serum reduces the apoptotic index of gonad granulocyte, improves ovary tissue anti-apoptotic substance
The expression of Bcl-2, so that the ratio of Bcl-2 and Bax are improved, to play the role of inhibiting apoptosis.
Detailed description of the invention
Fig. 1 is the content of catalase in separate groups of mice ovary tissue.As it can be seen that hydrogen water group Mouse Ovary Tissues in figure
Catalase content is higher than naive mice, but there was no significant difference.Mould hydrogen group Mouse Ovary Tissues catalase content
Higher than model group mouse, but there was no significant difference.
Fig. 2 is the concentration of Anti-Mullerian hormone in separate groups of mice serum.As it can be seen that the anti-Miao Le of model group mice serum in figure
The concentration of pipe hormone is significantly lower than naive mice, and has significant difference.Mould hydrogen group mice serum Anti-Mullerian hormone is dense
Degree is apparently higher than model group mouse, and has significant difference.Hydrogen water group mice serum Anti-Mullerian hormone concentration is apparently higher than
Model group mouse, and there are significant differences.It is small that mould hydrogen group mice serum Anti-Mullerian hormone concentration is slightly below blank group
Mouse, there was no significant difference.
Fig. 3 is the apoptotic index of separate groups of mice gonad granulocyte.As it can be seen that model group mouse granulosa cells in figure
Apoptotic index is apparently higher than blank group, hydrogen water group, mould hydrogen group, and all has extremely significant difference.Mould hydrogen group mouse ovarian
Granulocyte apoptotic index is higher than hydrogen water group, and has significant difference.Mould hydrogen group mouse granulosa cells apoptotic index is slightly higher
In blank group, there was no significant difference.
Fig. 4 is the expression of separate groups of mice ovary tissue Bcl-2.In figure as it can be seen that in mould hydrogen group Mouse Ovary Tissues
Bcl-2 level is apparently higher than model group mouse.Bcl-2 level is apparently higher than naive mice in hydrogen water group Mouse Ovary Tissues.
Fig. 5 is the expression of separate groups of mice ovary tissue Bax.As it can be seen that Bax in model group Mouse Ovary Tissues in figure
Level is slightly above other three groups of mouse, but difference is not obvious.Blank, hydrogen water, Bax is horizontal in three groups of Mouse Ovary Tissues of mould hydrogen
Have no notable difference.
Asterisk and pound sign indicate that there are significant difference, " * " or " # " to represent p < 0.05 between two groups, " * * " or " ## " generation
Table p < 0.01；" * * * " or " ### " represent p < 0.001.
Specific embodiment
Allow mouse long-term drinking hydrogen-rich water, free water.
Claims (1)
1. hydrogen-rich water is being prepared for protecting or preventing or alleviating the application in premature ovarian failure mouse ovarian damage medicine,
It is characterized in that,
The drug is oral drugs,
The drug is used to change and improve the concentration of catalase in ovary tissue；
Improve the concentration of Anti-Mullerian hormone AMH in serum；
Reduce gonad granulocyte apoptotic index, improve ovary tissue anti-apoptotic substance B cl-2 expression, improve Bcl-2 and
The ratio of Bax.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510418601.9A CN106333959B (en) | 2015-07-17 | 2015-07-17 | Protective effect of the hydrogen-rich water to premature ovarian failure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510418601.9A CN106333959B (en) | 2015-07-17 | 2015-07-17 | Protective effect of the hydrogen-rich water to premature ovarian failure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106333959A CN106333959A (en) | 2017-01-18 |
| CN106333959B true CN106333959B (en) | 2019-11-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510418601.9A Active CN106333959B (en) | 2015-07-17 | 2015-07-17 | Protective effect of the hydrogen-rich water to premature ovarian failure |
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| CN (1) | CN106333959B (en) |
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2015
- 2015-07-17 CN CN201510418601.9A patent/CN106333959B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 富氢水制备及保存方法的初步研究;秦秀军，等;《癌变畸变突变》;20131130;第25卷(第6期);第457-460页，尤其是第458页左栏第2段 * |
| 氢盐水对化疗药物致卵巢损伤的影响;孟啸寅;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20140215(第2014年第2期);摘要，正文第20-24页 * |
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| CN106333959A (en) | 2017-01-18 |
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Effective date of registration: 20170509 Address after: 100026 Beijing city Chaoyang District Yaojieyuan Road No. 251 Applicant after: Beijing Obstetrics and Gynecology, Capital University of Medical Science Address before: 100026 Beijing city Chaoyang District Chaoyang District Yaojieyuan Road No. 251 Applicant before: Ma Yanmin Applicant before: He Xin |
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