CN106318849B - A kind of Cell vibration meter - Google Patents
A kind of Cell vibration meter Download PDFInfo
- Publication number
- CN106318849B CN106318849B CN201610945337.9A CN201610945337A CN106318849B CN 106318849 B CN106318849 B CN 106318849B CN 201610945337 A CN201610945337 A CN 201610945337A CN 106318849 B CN106318849 B CN 106318849B
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- connecting shaft
- vibration
- motor
- eccentric disc
- guide rail
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Percussion Or Vibration Massage (AREA)
- Apparatuses For Generation Of Mechanical Vibrations (AREA)
Abstract
The invention discloses a kind of Cell vibration meters, and including pedestal, pedestal is equipped with motor fixing frame and vibration frame, and motor is fixed on motor fixing frame;Horizontal straight slot is offered in vibration frame, is equipped with the first connecting shaft in horizontal straight slot, one end of motor and the first connecting shaft is rotated synchronously by synchronous belt to be connected, and the other end of the first connecting shaft is connected with vibration component;Vibration component includes eccentric disc, the second connecting shaft, upright guide rail, vertical connecting rod, L-shaped and lifts platform and fixed disk, eccentric disc is connected in the first connecting shaft, vibration frame is equipped with upright guide rail, upright guide rail is equipped with sliding block, the both ends of second connecting shaft are connect respectively with eccentric disc and vertical connecting rod, L-shaped lifts platform clamping connection between sliding block and fixed disk, and fixed disk is connect with vertical connecting rod.The motor of the Cell vibration meter can be connected directly with power supply, and power good is not interfered by extraneous factor, suitable for being vibrated outside histiocytic incubator.
Description
Technical field
The invention belongs to Bioexperiment Instrument technology fields, and in particular to a kind of Cell vibration meter.
Background technology
In order to achieve the purpose that cell tissue crushes, generally require that vibration gauge can be used, because vibration gauge can be with high-frequency
Vibration, such cell tissue cell tissue under the impact of high-energy can crush rapidly, be a kind of effective sample pre-treatments
Equipment.
However the vertical vibration instrument on existing market is mostly large-scale desk-top vibration gauge, is vibrated including human body vibration instrument, animal
Instrument, these vibration gauges are bulky, are not suitable for the small cell of volume.It is a kind of small suitable for volume it is therefore necessary to develop
Cell or tissue vibration gauge.
However existing minicell vibration gauge is carried out in cell incubator, operation difficulty is big, and Oscillation Amplitude is small, right
In the larger tissue of vibration demand and do not apply to.
Invention content
The object of the present invention is to provide a kind of Cell vibration meters, and solving existing minicell vibration gauge is trained in cell
It supports and is carried out in case, operation difficulty is big, and Oscillation Amplitude is small, the problem of for the larger tissue of vibration demand and not applying to.
A kind of Cell vibration meter provided by the invention, including pedestal, side is equipped with motor fixing frame on pedestal, and opposite side is set
There is vibration frame, the motor fixing frame is equipped with dismountable motor;
Horizontal straight slot is offered in the vibration frame, the first connecting shaft is equipped with, and described in the horizontal straight slot
It is rotatablely connected between first connecting shaft and the horizontal straight slot by clutch shaft bearing, the motor and one end of the first connecting shaft lead to
It crosses synchronous belt and rotates synchronously connection, the other end of first connecting shaft is connected with vibration component;
The vibration component includes eccentric disc, the second connecting shaft, upright guide rail, vertical connecting rod, L-shaped and lifts platform and fixation
Disk, the eccentric disc are removably connected to the one end of the first connecting shaft far from synchronous belt, and the vibration frame is equipped with can
The upright guide rail of dismounting, and upright guide rail is located at the top of the eccentric disc, and the upright guide rail is equipped with along upright guide rail
The sliding block of slip offers several eccentric orfices on the eccentric disc, and one end of the vertical connecting rod offers bearing groove, the axis
It holds slot and is embedded with second bearing, one end of second connecting shaft is plugged in one of eccentric orfice, and the other end is plugged in
In two bearings, the vertical end that the L-shaped lifts platform is connected to by the first bolt clip between the sliding block and fixed disk, horizontal ends position
In the top of the vertical connecting rod, and it is detachably connected between the fixed disk and the vertical connecting rod by screw rod, it is described
One end periphery far from fixed disk on screw rod is connected with fixed circlip.
Preferably, the control circuit for controlling the motor intermittence rotation is also electrically connected on the motor.
Preferably, first pulley is fixed on the output shaft of the motor, first connecting shaft is close to synchronous belt
One end is fixed with the second belt wheel, is rotated synchronously and connected by synchronous belt between the first pulley and the second belt wheel.
Preferably, the quantity of the clutch shaft bearing is 2, and is located at the both sides of the horizontal straight slot respectively.
Preferably, the first connecting shaft outer cover is equipped with sleeve, and the sleeve is set on the second belt wheel and bobbing machine
Between frame.
Preferably, first connecting shaft is equipped with fixed plate close to one end of eccentric disc, and the eccentric disc passes through the second spiral shell
It tethers and is connected in fixed plate.
Preferably, the clutch shaft bearing and second bearing are deep groove ball bearing.
Preferably, the motor is bolted to by third on motor fixing frame, and the upright guide rail passes through the 4th spiral shell
It tethers and is connected on vibration frame.
Preferably, the L-shaped, which is lifted, is equipped with dismountable buffering clamp spring, the buffering clamp between platform and the vertical connecting rod
Spring is the elastic circlip with gap, and elastic circlip is connected on the first bolt.
The present invention Cell vibration meter, motor can directly with 380V three phase mains connect, power good, not by it is extraneous because
The interference of element, suitable for being vibrated outside the larger histiocytic incubator of volume;By the control circuit that is connected on motor to electricity
Machine frequency control can then realize the adjusting that platform vibration frequency is lifted to L-shaped;By setting dismountable eccentric disc, to realize
Amplitude it is separately adjustable;The Cell vibration meter of the present invention is designed using non-close, detachable to protect convenient for the replacement of each component
It deposits, saves memory space.
Description of the drawings
Fig. 1 is the structure diagram of Cell vibration meter of the present invention;
Fig. 2 is the front view of the structure diagram of Cell vibration meter of the present invention;
Fig. 3 is the vertical view of the structure diagram of Cell vibration meter of the present invention;
Fig. 4 is the structure diagram of eccentric disc in Cell vibration meter of the present invention;
Fig. 5 is the structure diagram of vibration component in Cell vibration meter of the present invention;
Fig. 6 is that Cell vibration meter of the present invention is used for the control circuit that motor intermittence is controlled to rotate.
Reference sign:1. pedestal, 2. motor fixing frames, 21. motors, 22. first pulleys, 3. vibration frames, 31. water
Flat straight slot, 32. first connecting shafts, 33. clutch shaft bearings, 34. second belt wheels, 35. sleeves, 36. flat keys, 4. synchronous belts, 5. partially
Cartridge, 51. eccentric orfices, 52. second connecting shafts, 6. upright guide rails, 61. sliding blocks, 7. vertical connecting rods, 71. bearing grooves, 72. second
Bearing, 8.L shapes lift platform, and 81. fix disks, 82. fixed circlips, 9. first bolts, 91. second bolts, 92. third bolts, and 93.
4th bolt.
Specific embodiment
Below in conjunction with the accompanying drawings, the specific embodiment of the present invention is described in detail, it is to be understood that the guarantor of the present invention
Shield range is not restricted by specific implementation.
A kind of Cell vibration meter, specifically as shown in Figs. 1-5, including pedestal 1, side is equipped with motor fixing frame 2 on pedestal 1,
Opposite side is equipped with vibration frame 3, and the motor fixing frame 2 is equipped with dismountable motor 21, and motor fixing frame 2 passes through third spiral shell
Bolt 92 is connected with motor 21, and to realize the detachable of motor 21, motor 21 is electrically connected with to control the intermittent rotation of motor 21
Control circuit;
Horizontal straight slot 31 is offered in vibration frame 3, the first connecting shaft 32 is equipped in horizontal straight slot 31, and first connects
It is rotatablely connected between spindle 32 and horizontal straight slot 31 by clutch shaft bearing 33, the quantity of clutch shaft bearing 33 is 2, and position respectively
At the both sides of the edge of horizontal straight slot 31, in order to which the position of the first connecting shaft 32 is relatively fixed, make it along clutch shaft bearing 33
Stable rotation, one end of 21 and first connecting shaft 32 of motor is realized by synchronous belt 4 rotates synchronously connection, the first connecting shaft
32 other end is connected with vibration component, and 32 outer cover of the first connecting shaft is equipped with sleeve 35, and sleeve 35 is set on the second belt wheel
Between 34 and vibration frame 3;
Vibration component includes eccentric disc 5, the second connecting shaft 52, upright guide rail 6, vertical connecting rod 7, L-shaped and lifts platform 8 and fix
Disk 81, the first connecting shaft 32 are equipped with fixed plate close to one end of eccentric disc 5, and eccentric disc 5 is connected to fixation by the second bolt 91
On plate, eccentric disc 5 is removably connected to first one end of connecting shaft 32 far from synchronous belt 4, in order to be carried out to eccentric disc 5
It replaces;
Dismountable upright guide rail 6 is connected with by the 4th bolt 93 on vibration frame 3, and upright guide rail 6 is located at partially
The top of cartridge 5, upright guide rail 6 are equipped with the sliding block 61 that can be slided up and down along upright guide rail 6,2 bias are offered on eccentric disc 5
Hole 51, one end of vertical connecting rod 7 offer bearing groove 71, and bearing groove 71 is embedded with second bearing 72, and the one of the second connecting shaft 52
End is plugged in one of eccentric orfice 51, and the other end is plugged in second bearing 72, to realize that vertical connecting rod 7 removably connects
It is connected on eccentric disc 5, L-shaped lifts the vertical end of platform 8 by 9 clamping connection of the first bolt between sliding block 61 and fixed disk 81, horizontal
End offers first level tapped through hole, 81 and first water of fixed disk on the top of the vertical connecting rod 7, vertical connecting rod 7
The opposite position of flat thread through-hole offers the second horizontal screw thread through-hole, and the first flat thread through-hole leads to the second horizontal screw thread
Hole matches with the both ends of screw rod be spirally connected respectively, is detachably connected between fixed disk 81 and vertical connecting rod 7 by screw rod, the two of screw rod
End is bolted in respectively in first level tapped through hole and the second horizontal screw thread through-hole, is detachably connected with realizing, separate on screw rod
One end periphery of fixed disk 81 is connected with fixed circlip 82, for limiting screw rod activity.
The Cell vibration meter of the present invention, drives the first connecting shaft 32 to rotate by 21 synchronized belt 4 of motor, and then drives inclined
Cartridge 5 rotates, and last band movable slider 61 is up and down reciprocatingly slided along upright guide rail 6, and the velocity of rotation of eccentric disc 5 is that L-shaped is lifted
The vibration frequency of platform 8 during experiment, sample to be vibrated is positioned in suitable container, then lifts platform 8 being positioned over L-shaped
On, complete the vibration processing of sample.Preferably, motor 21 be threephase asynchronous machine, threephase asynchronous machine directly with 380V three-phases
Power supply is connected, and power good is not interfered by extraneous factor, by that threephase asynchronous machine frequency control, then can realize to L
Shape lifts the adjusting of 8 vibration frequency of platform.
Sleeve 35 for the second belt wheel 34 and vibration frame 3 to be separated, when preventing the second belt wheel 34 from rotating with vibration frame 3
It causes to rub, while the first connecting shaft 32 can also be made to reduce abrasion, increase the flexibility of device.
It should be noted that for controlling the control circuit of the intermittent rotation of motor 21, what the control circuit was selected is
The circuit that can realize the 21 intermittent rotation of control motor of the prior art, such as control circuit shown in fig. 6.
Control circuit connecting components according to figure 6 during experiment, are first closed power switch Q, and frequency converter is (existing at this time
There are the frequency converter of technology, such as the frequency converter of upper seamount space SY8000 series, SY8000-0R7G-2, SY8000-1R5G-
2) it is powered, adjusted frequency values is manually entered by the control panel of frequency converter, press operation button.Control circuit is set to position
In in switch board, pressing the start button SB on switch board1, at this point, relay K1Coil obtain electric, normally opened contact K10It closes
It closes, A.C. contactor KM coils obtain electric, main contacts closure, and main circuit is connected at this time, the frequency modulation electricity that motor 21 is exported in frequency converter
Pressure is started to work, and L-shaped is driven to lift platform shake table and starts to vibrate.Time relay KT1Coil be also powered, and start to count
When, while relay K2Coil obtain electric, normally-closed contact K21It disconnects.Elapsed time t1Afterwards, time relay KT1Normally opened prolong
When contact KT10It is closed, normally closed delayed contact KT11It disconnects, relay K2Coil blackout, normally-closed contact K21It is closed, therefore after
Electric appliance K3Coil be powered, normally-closed contact K31It disconnects, normally opened contact K30It is closed, at this time A.C. contactor KM coil blackouts, it is main
Contact disconnects, and main circuit disconnects, and motor 21 stops, and L-shaped lifts platform shake table and also stops vibrating.Time relay KT2Coil
It is powered, and starts timing, while relay K4Coil obtain electric, normally-closed contact K41It disconnects.Elapsed time t2Afterwards, time relay
Device KT2Normally opened delayed contact KT20It is closed, normally closed delayed contact KT21It is closed, relay K4Coil blackout, normally-closed contact
K41It is closed, therefore relay K1Coil be powered, normally opened contact K10It being closed, A.C. contactor KM coils are once again powered up at this time,
So that main circuit is again switched on, start working cycles next time.When by lower button SB0, control circuit power-off, disconnection is electric at this time
Source switch Q, L-shaped lifts platform, and also vibration mechine is stopped.
L-shaped lifts the vibration time t of platform1With intermittent time t2By two time relay KT in control circuit1And KT2
It is adjusted respectively, selects DH48S-S digital display time relays, delay time can be adjusted between 0.1s to 99h, can
Fully meet the requirement of vibration test.
Relay K1、K2、K3、K4Using the relay of the prior art, such as JT4 series alternating current electromagnetic relays.
Pass through fuse FU between power switch Q and frequency converter1Connection, while lead between A.C. contactor KM and ground wire
Cross fuse FU2Connection.
It should be noted that eccentric disc 5 is the first eccentric disc, the second eccentric disc, third eccentric disc or the 4th eccentric disc,
2 eccentric orfices 51 are offered on one eccentric disc, the second eccentric disc, third eccentric disc and the 4th eccentric disc;Wherein first is eccentric
2 eccentric orfices, the 51 corresponding eccentricity of disk is respectively 1mm, 8mm;2 eccentric orfices, the 51 corresponding eccentricity of second eccentric disc
Respectively 2mm, 7mm;2 eccentric orfices, the 51 corresponding eccentricity of third eccentric disc is respectively 3mm, 6mm;The 2 of 4th eccentric disc
A 51 corresponding eccentricity of eccentric orfice is respectively 4mm, 5mm.So as to realize the independence of various amplitude by replacing eccentric disc 5
It adjusts, is not interfered and changed by other factors, amplitude is adjustable in the range of 1-8mm, resolution ratio 1mm.
When eccentric disc 5 is replaced, fixed circlip 82 is removed, removes the screw rod, by vertical connecting rod 7 together with the second connecting shaft 52
It extracts out of eccentric orfice 51 together, at this moment eccentric disc 5 is exposed to outside, removes the second bolt 91 for fixing eccentric disc 5 i.e.
The replacement of eccentric disc 5 can be carried out, after replacement, by each member reset, you can continue the vibration of cell tissue.
It should be noted that being fixed with first pulley 22 on the output shaft of motor 21, the first connecting shaft 32 is close to synchronous skin
The second belt wheel 34 is fixed with 4 one end, is turned between 22 and second belt wheel 34 of first pulley by the way that the realization of synchronous belt 4 is synchronous
Dynamic connection.First pulley 22 can amplify the rotation amplitude of 21 output shaft of motor, and the second belt wheel 34 can be by amplified rotation
Amplitude is driven the working efficiency that the first connecting shaft 32, can either increase motor 21 in this way, can also reduce the output shaft of motor 21
With the abrasion of the first connecting shaft 32, increase its service life.
It should be noted that in order to increase connective stability of second belt wheel 34 in the first connecting shaft 32, the second belt wheel
Offer the first groove on 34, the position opposite with the first groove offers the second groove, the first groove in the first connecting shaft 32
A square groove is collectively constituted with the second groove, square groove is embedded with flat key 36, for preventing the second belt wheel 34 relative to first
Connecting shaft 32 is slided.
It should be noted that clutch shaft bearing 33 and second bearing 72 are deep groove ball bearing, make at the component respectively connected
In optimum rotation state.
It should be noted that L-shaped, which is lifted, is equipped with dismountable buffering clamp spring, the buffering clamp between platform 8 and vertical connecting rod 7
Spring is the elastic circlip with gap, and elastic circlip is connected on the first bolt 9, can be by breaking buffering clamp into two with one's hands by gap
Spring with the vibrating effect of buffer motor 21, plays the role of auxiliary adjustment vibrations.
Although preferred embodiments of the present invention have been described, but those skilled in the art once know basic creation
Property concept, then additional changes and modifications may be made to these embodiments.So appended claims be intended to be construed to include it is excellent
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (9)
1. a kind of Cell vibration meter, which is characterized in that including pedestal (1), side is equipped with motor fixing frame (2) on pedestal (1), separately
Side is equipped with vibration frame (3), and the motor fixing frame (2) is equipped with dismountable motor (21);
Horizontal straight slot (31) is offered in the vibration frame (3), the first connecting shaft is equipped in the horizontal straight slot (31)
(32), it and between first connecting shaft (32) and the horizontal straight slot (31) is rotatablely connected by clutch shaft bearing (33), institute
It states motor (21) and one end of the first connecting shaft (32) rotates synchronously connection, first connecting shaft by synchronous belt (4)
(32) the other end is connected with vibration component;
The vibration component includes eccentric disc (5), the second connecting shaft (52), upright guide rail (6), vertical connecting rod (7), L-shaped and lifts
Platform (8) and fixed disk (81), the eccentric disc (5) are removably connected to the separate synchronous belt (4) of the first connecting shaft (32)
One end, the vibration frame (3) is equipped with dismountable upright guide rail (6), and upright guide rail (6) is positioned at the eccentric disc
(5) top, the upright guide rail (6) are equipped with the sliding block (61) slided along upright guide rail (6), are opened on the eccentric disc (5)
Equipped with several eccentric orfices (51), one end of the vertical connecting rod (7) offers bearing groove (71), and the bearing groove (71) is embedded with
Second bearing (72), one end of second connecting shaft (52) are plugged in one of eccentric orfice (51), and the other end is plugged in
In second bearing (72), the L-shaped is lifted the vertical end of platform (8) and in the sliding block (61) and is consolidated by the first bolt (9) clamping connection
Between price fixing (81), horizontal ends are located at the top of the vertical connecting rod (7), the fixed disk (81) and the vertical connecting rod (7)
Between be detachably connected by screw rod, one end periphery far from fixed disk (81) on the screw rod is connected with fixed circlip (82).
2. Cell vibration meter according to claim 1, which is characterized in that be also electrically connected with to control on the motor (21)
Make the control circuit of the intermittent rotation of the motor (21).
3. Cell vibration meter according to claim 2, which is characterized in that is fixed on the output shaft of the motor (21)
One belt wheel (22), first connecting shaft (32) are fixed with the second belt wheel (34) close to one end of synchronous belt (4), and described first
It is rotated synchronously and connected by synchronous belt (4) between belt wheel (22) and the second belt wheel (34).
4. Cell vibration meter according to claim 3, which is characterized in that the quantity of the clutch shaft bearing (33) is 2, and
And it is located at the both sides of the horizontal straight slot (31) respectively.
5. Cell vibration meter according to claim 3, which is characterized in that the first connecting shaft (32) outer cover is equipped with set
Cylinder (35), and the sleeve (35) is between the second belt wheel (34) and vibration frame (3).
6. Cell vibration meter according to claim 3, which is characterized in that first connecting shaft (32) is close to eccentric disc
(5) one end is equipped with fixed plate, and the eccentric disc (5) is connected to by the second bolt (91) in fixed plate.
7. Cell vibration meter according to claim 2, which is characterized in that the clutch shaft bearing (33) and second bearing (72)
It is deep groove ball bearing.
8. Cell vibration meter according to claim 2, which is characterized in that the motor (21) is connected by third bolt (92)
It is connected on motor fixing frame (2), the upright guide rail (6) is connected to by the 4th bolt (93) on vibration frame (3).
9. Cell vibration meter according to claim 2, which is characterized in that the L-shaped lifts platform (8) and the vertical connecting rod
(7) it is equipped with dismountable buffering clamp spring between, the buffering clamp spring is the elastic circlip with gap, and elastic circlip is connected in the
On one bolt (9).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610945337.9A CN106318849B (en) | 2016-10-26 | 2016-10-26 | A kind of Cell vibration meter |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610945337.9A CN106318849B (en) | 2016-10-26 | 2016-10-26 | A kind of Cell vibration meter |
Publications (2)
Publication Number | Publication Date |
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CN106318849A CN106318849A (en) | 2017-01-11 |
CN106318849B true CN106318849B (en) | 2018-07-03 |
Family
ID=57818902
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CN201610945337.9A Expired - Fee Related CN106318849B (en) | 2016-10-26 | 2016-10-26 | A kind of Cell vibration meter |
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CN (1) | CN106318849B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113174313A (en) * | 2021-05-10 | 2021-07-27 | 江苏绿科生物技术有限公司 | Microbial cultivation device |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD205824A1 (en) * | 1982-07-01 | 1984-01-11 | Adw Ddr | DEVICE FOR VIBRATION CULTIVATION |
US5564826A (en) * | 1995-09-27 | 1996-10-15 | Robbins Scientific Corporation | Reciprocating bath shaker |
CN201300118Y (en) * | 2008-09-20 | 2009-09-02 | 程云 | Vehicular blood corpuscle oscillation instrument |
CN101852695B (en) * | 2010-04-30 | 2011-11-30 | 宁波新芝生物科技股份有限公司 | High-flux tissue grinder |
CN103013827B (en) * | 2013-01-18 | 2015-03-11 | 辽宁医学院 | Cell vibration meter |
CN105251399A (en) * | 2015-11-04 | 2016-01-20 | 上海大学 | Linear reciprocating/circumferential oscillating mechanism |
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2016
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Granted publication date: 20180703 Termination date: 20181026 |