CN106318606A - Method for preparing biodiesel from algae as raw material - Google Patents

Method for preparing biodiesel from algae as raw material Download PDF

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Publication number
CN106318606A
CN106318606A CN201610772842.8A CN201610772842A CN106318606A CN 106318606 A CN106318606 A CN 106318606A CN 201610772842 A CN201610772842 A CN 201610772842A CN 106318606 A CN106318606 A CN 106318606A
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biodiesel
algae
buttress shaft
raw material
cylindrotheca
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黄健峻
其他发明人请求不公开姓名
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Nanning Overseas Chinese Investment Zone Sun Trading Co Ltd
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Nanning Overseas Chinese Investment Zone Sun Trading Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C11B1/108—Production of fats or fatty oils from raw materials by extracting after-treatment, e.g. of miscellae
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C1/00—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids
    • C11C1/08—Refining
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C1/00—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids
    • C11C1/08—Refining
    • C11C1/10—Refining by distillation
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • C11C3/04—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils
    • C11C3/10—Ester interchange
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Liquid Carbonaceous Fuels (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)

Abstract

The invention discloses a method for preparing biodiesel from algae as a raw material. The method comprises the following steps: a medium is inoculated with cylindrotheca, the cylindrotheca is irradiated with red and blue LED light supplement lamps for 20-24 h/day and cultured at a temperature of 24-27 DEG C for 5-8 days, and cylindrotheca cells can be collected; the collected cylindrotheca cells are subjected to wall breaking by ultrasonic waves and then extracted with an organic solvent, microalgal oil is prepared, the microalgal oil, methanol and a zeolite molecular sieve catalyst are then added to a reactor for an esterification reaction in a mass ratio of 1:(4-6):(0.02-0.04), an esterification product is obtained and then is separated and purified, and biodiesel can be obtained. According to the method, the cylindrotheca which is short in production cycle, widely distributed, high in photosynthesis and high in oil content is adopted as the raw material, and the problem of plant oil raw materials in biodiesel development is solved. Meanwhile, the method has the advantages of simple preparation process, high catalytic efficiency, high oil extraction rate, high product yield and the like, and good basis is provided for industrialization and scale production of the biodiesel.

Description

A kind of be raw material production biodiesel with algae method
Technical field
The invention belongs to biomass energy technology field, a kind of with algae for raw material production biology bavin specifically The method of oil.
Background technology
Along with the exhaustion day by day of fossil fuel, oil price persistently sharp rises, and seeking regenerative resource has become solution Human survival and the needs of holding World Economics sustainable development, the biodiesel as novel alternative energy source is sent out in countries in the world Exhibition is rapidly.Biodiesel refers to oil crop such as Semen sojae atricolor, Brassica campestris L, cotton, Petiolus Trachycarpi etc., the water such as Wild oil plant and engineering microalgae Raw vegetable oil fat and animal oil, food garbage oil etc. for raw oil by replacing that ester exchange or thermochemical processes are made The reproducibility diesel fuel of petrifaction diesel.The advantage of biodiesel is not limited only to recyclability, compared with existing diesel oil, also has Following advantage: Cetane number is high so that combustibility is more preferable；Preferably wettability, makes injection pump, engine cylinder-body and connecting rod Abrasion reduce, increase the service life；In biodiesel, sulfur content is low, and the discharge of sulfur dioxide and sulfide is low, reduces environment Pollute；Biodiesel flash-point is high, is not belonging to dangerous materials, all has superiority in transport, storage, use etc..
At present, China's industrialized production biodiesel is mainly with Oleum Brassicae campestris, Oleum Gossypii semen, Chinese tallow, shinyleaf yellowhorn oil, Oleum Camelliae It is raw material with waste oil etc..But the limits throughput of these raw materials, the biodiesel of production can only meet current vehicle demand for fuel The 0.3% of amount.And a large amount of Vegetable oil lipoprotein produces biodiesel, anxiety and the crops price of land resource will certainly be caused Rise, especially in the case of China has a large population and a few land, these are particularly problematic.In this case, microalgae biodiesel The concern of more and more people has been attracted with the advantage of its uniqueness.Microalgae, as a kind of important biodiesel raw material, is planted with oil plant Thing is compared, and has that distribution is wide, Biomass high, photosynthetic efficiency is high, adaptive capacity to environment is strong, growth cycle is short, fat content is high, becomes The significant advantages such as this lowest and environmental friendliness.
In recent years, research prepared by relevant biodiesel is concentrated mainly on the aspect such as woody oleiferous plants and disposed waste oil, from Microalgae extracts oils and fats little for the research report preparing biodiesel.Wherein, biodiesel one is prepared with microalgae for raw material As comprise the following steps that: Strain selection, pilot scale culture, extraction oils and fats, esterification prepare biodiesel coarse product, warp Cross refine and i.e. can get biodiesel.The microalgae kind selecting fat content high is to prepare the key of biodiesel, cultivates simultaneously Base and envirment factor also have important impact to the fat content of microalgae.Therefore, how to improve the nutritional labeling of culture medium and change The changing environment factor is one of approach improving algae oils and fats productivity.Although improving microalgae fat content can be that biodiesel is laid well Basis, but the production technology of biodiesel is most important to its yield effect.The production technology of existing biodiesel is general There is problems in that catalytic efficiency is low, yield of biodiesel is low, response time length, etching apparatus, complex process, energy consumption are relatively big, Relatively costly deficiency such as grade.
Buttress shaft algae (Cylindrotheca) is a kind of marine diatom, and this kind has bank property, and distributed pole is wide.The south of China Sea, the East Sea and the Huanghai Sea all have distribution, and fat content is up to 25-30%, mainly contains C in lubricant component18、C16The fat of carbochain Acid glyceride, close with the biodiesel fuel component extracted from vegetable oil, it is the class bioenergy material with potentiality to be exploited.
Summary of the invention
The present invention is directed to the problem that Vegetable oil lipoprotein limits throughput and existing biodiesel oil preparing process exist, it is provided that a kind of with Algae is the method for raw material production biodiesel.The inventive method has that raw material sources are extensive, catalytic efficiency is high, power consumption The advantage such as low, biodiesel production rate is high, preparation technology is simple, lays a good foundation for biodiesel industrialization, scale, Can effective alleviating energy crisis problem.
In order to realize object above, the present invention is to be achieved through the following technical solutions:
A kind of with algae for the method for raw material production biodiesel, comprise the following steps:
S1. buttress shaft algae is cultivated: with buttress shaft algae as raw material, then is seeded to training with the inoculum density of ten thousand cell/mL of 300-500 Support base, irradiate 20-24h/ days with red blue dual-colored LED light compensating lamp, control intensity of illumination and control in 4000-5000LX, cultivation temperature At 24-27 DEG C, cultivating 5-8 days, the density of buttress shaft algae reaches concentration and reaches 70,000,000 cell/mL, collects buttress shaft frustule；Institute The culture medium stated is composed of the following components: glucose 30-40mg/L, NaNO3 1.2-1.4mg/L、KH2PO41.2-1.4mg/L、 Chitosan 1.0-1.4mg/L, biogas slurry 6-8mg/L, f2 vitamin 3-4mL and sea water 1L；
S2. oils and fats extracts: after step S1 is collected buttress shaft frustule concentrate drying, uses ultrasound wave to break buttress shaft algae Wall processes, and obtains buttress shaft algae powder, and adding in buttress shaft algae powder with its mass ratio is the petroleum ether of 1:9-12, and mixing stands 3-7h, on Layer liquid is oils and fats extracting solution, then by extracting solution 80-85 DEG C of distillation, i.e. can get microalgae grease；
S3. ester exchange reaction: be 1:4-6 according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst: The ratio of 0.02-0.04 adds in reactor, at temperature is 55-70 DEG C, carries out esterification 90-120min, is esterified Product；
S4. standing separation；By esterification products centrifugal 12-15min, stratification under rotating speed is 100-150r/min, divide Separating out upper strata glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 70-80 DEG C, Obtain biodiesel.
Further, the compound method of the f2 vitamin described in above step S1 is as follows: 0.5mg vitamin B12、0.5mg Biotin, 100mL vitamin B1 and pure water 1000mL.
Further, the HONGGUANG in above-described red blue dual-colored LED light compensating lamp is 1:2-3 with blue light proportioning, preferably joins Ratio is 1:3.
Further, using ultrasound wave that buttress shaft algae is carried out broken wall treatment described in above step S2, ultrasonic power is 150- 200W, ultrasonic time are 35-40min.
Compared with prior art, the beneficial effect that the present invention obtains:
1, the present invention prepares biodiesel with buttress shaft algae for raw material, can effectively alleviate current energy crisis and environmental pollution Problem, buttress shaft algae has that distribution is wide, Biomass high, photosynthetic efficiency is high, adaptive capacity to environment is strong, growth cycle is short, fat content The significant advantages such as height, low cost and environmental friendliness, it is possible to solve the Vegetable oil lipoprotein insufficient raw material in biodiesel exploitation, cost mistake The problems such as height.
2, the present invention adds in the medium chitosan and biogas slurry, containing nutrition such as substantial amounts of trace element, Organic substances Composition, can accelerate microalgae speed of production and improve the oils and fats productivity of microalgae.
3, the inventive method is by using red blue dual-colored LED light compensating lamp to change illumination condition, is possible not only to improve buttress shaft algae Photosynthesis, accelerate buttress shaft algae and produce and improve yield, the oils and fats productivity of buttress shaft algae can also be improved simultaneously, white with conventional Vehement lamp, fluorescent lamp or monochromatic LED lamp are compared, and the oils and fats productivity of buttress shaft algae improves about 5%.
4, the inventive method uses supersonic wave wall breaking method, and ultrasound wave can produce unique mechanical vibration to microalgae cell Effect and cavitation, advantages such as having that sporoderm-broken rate is high, process the time period, environmentally safe, power consumption are low, improve oils and fats Extraction ratio.
5, the inventive method uses zeolite molecular sieve as catalyst, possesses that reaction interface is big, surface area big, resistance to mass tranfer Little, reaction condition is gentle, active height, pollution-free, the not feature such as etching apparatus, improve catalytic efficiency, shorten esterification Time, catalytic effect is notable, improves the yield of biodiesel.
6, the inventive method have that preparation technology is simple, catalytic efficiency is high, grease extraction efficiency is high, biodiesel production rate is high, Product purity is high, raw material sources extensively, the advantage such as low cost, power consumption be low, there is good society, ecology and economic benefit, for Microalgae biodiesel industrialization, scale are laid a good foundation.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but is not limited to protection scope of the present invention.
Embodiment 1
The preparation of buttress shaft algae culture medium: glucose 30mg/L, NaNO3 1.4mg/L、KH2PO41.2mg/L, chitosan 1.0mg/L, biogas slurry 6mg/L, f2 vitamin 3mL and sea water 1L；
A kind of with algae for the method for raw material production biodiesel, comprise the following steps:
S1. buttress shaft algae is cultivated: with buttress shaft algae as raw material, then is seeded to cultivate with the inoculum density of 5,000,000 cell/mL Base, irradiates 20h/ days with red blue dual-colored LED light compensating lamp, and in LED light supplement lamp, HONGGUANG and blue light proportioning are 1:2, control intensity of illumination At 5000LX, cultivation temperature controls at 27 DEG C, cultivates 7 days, and the density of buttress shaft algae reaches concentration and reaches 70,000,000 cell/mL, Collect buttress shaft frustule；
S2. oils and fats extracts: after step S1 is collected buttress shaft frustule concentrate drying, uses ultrasound wave to break buttress shaft algae Wall processes, and control ultrasonic power is 150W, and ultrasonic reaction 35min obtains buttress shaft algae powder, adds and its mass ratio in buttress shaft algae powder For the petroleum ether of 1:9, mixing stands 7h, and upper liquid is oils and fats extracting solution, then by extracting solution 80 DEG C of distillations, i.e. can get micro- Algae oils and fats；
S3. ester exchange reaction: be 1:4:0.04's according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst Ratio adds in reactor, at temperature is 55 DEG C, carries out esterification 90min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 15min, stratification under rotating speed is 100r/min, isolate upper strata Glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 70 DEG C, To biodiesel.
Embodiment 2
The preparation of buttress shaft algae culture medium: glucose 35mg/L, NaNO3 1.3mg/L、KH2PO41.3mg/L, chitosan 1.2mg/L, biogas slurry 7mg/L, f2 vitamin 4mL and sea water 1L；
A kind of with algae for the method for raw material production biodiesel, comprise the following steps:
S1. buttress shaft algae is cultivated: with buttress shaft algae as raw material, then is seeded to cultivate with the inoculum density of 4,000,000 cell/mL Base, irradiates 22h/ days with red blue dual-colored LED light compensating lamp, and in LED light supplement lamp, HONGGUANG and blue light proportioning are 1:3, control intensity of illumination At 4500LX, cultivation temperature controls at 24 DEG C, cultivates 5 days, and the density of buttress shaft algae reaches concentration and reaches 70,000,000 cell/mL, Collect buttress shaft frustule；
S2. oils and fats extracts: after step S1 is collected buttress shaft frustule concentrate drying, uses ultrasound wave to break buttress shaft algae Wall processes, and control ultrasonic power is 170W, and ultrasonic reaction 40min obtains buttress shaft algae powder, adds and its mass ratio in buttress shaft algae powder For the petroleum ether of 1:10, mixing stands 3h, and upper liquid is oils and fats extracting solution, then by extracting solution 82 DEG C of distillations, the most available Microalgae grease；
S3. ester exchange reaction: be 1:5:0.03's according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst Ratio adds in reactor, at temperature is 60 DEG C, carries out esterification 100min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 13min, stratification under rotating speed is 120r/min, isolate upper strata Glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 80 DEG C, To biodiesel.
Embodiment 3
The preparation of buttress shaft algae culture medium: glucose 40mg/L, NaNO3 1.2mg/L、KH2PO41.4mg/L, chitosan 1.4mg/L, biogas slurry 8mg/L, f2 vitamin 3mL and sea water 1L；
A kind of with algae for the method for raw material production biodiesel, comprise the following steps:
S1. buttress shaft algae is cultivated: with buttress shaft algae as raw material, then is seeded to cultivate with the inoculum density of 3,000,000 cell/mL Base, irradiates 24h/ days with red blue dual-colored LED light compensating lamp, and in LED light supplement lamp, HONGGUANG and blue light proportioning are 1:2, control intensity of illumination At 4000LX, cultivation temperature controls at 25 DEG C, cultivates 8 days, and the density of buttress shaft algae reaches concentration and reaches 70,000,000 cell/mL, Collect buttress shaft frustule；
S2. oils and fats extracts: after step S1 is collected buttress shaft frustule concentrate drying, uses ultrasound wave to break buttress shaft algae Wall processes, and control ultrasonic power is 200W, and ultrasonic reaction 35min obtains buttress shaft algae powder, adds and its mass ratio in buttress shaft algae powder For the petroleum ether of 1:12, mixing stands 5h, and upper liquid is oils and fats extracting solution, then by extracting solution 85 DEG C of distillations, the most available Microalgae grease；
S3. ester exchange reaction: be 1:6:0.02's according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst Ratio adds in reactor, at temperature is 70 DEG C, carries out esterification 120min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 12min, stratification under rotating speed is 150r/min, isolate upper strata Glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 75 DEG C, To biodiesel.
The buttress shaft algae utilizing oils and fats extraction weight method to collect above example carries out fat content mensuration, result such as table Shown in 1.Wherein oils and fats total content=(microalgae grease/buttress shaft algae powder) × 100%.
Table 1. buttress shaft algae fat content
Oils and fats total content (%)
Embodiment 1 32
Embodiment 2 35
Embodiment 3 33
Biodiesel prepared by above example is according to GB/T20828-2007 (diesel fuel blending biodiesel) The performance of standard detection product, testing result is as shown in table 2.
The properties of product of the biodiesel of table 2. the present embodiment
Density (g/cm3) Viscosity (40 DEG C of mm2/s) Acid number (mgKOH/g) Flash-point (DEG C) Cetane number
Embodiment 1 0.86 3.46 0.42 161 53
Embodiment 2 0.84 3.65 0.36 159 67
Embodiment 3 0.87 3.53 0.43 153 65
Standard value 0.82-0.9 1.9-6.0 ≤0.8 ≥130℃ ＞ 49
As can be seen from the above table, the indices of biodiesel prepared by the present invention is above diesel fuel blending life The standard of thing diesel oil, good market prospects.
Above content is to combine concrete/the most made for the present invention further description, it is impossible to Assert the present invention be embodied as be confined to these explanations.General technical staff of the technical field of the invention is come Saying, without departing from the inventive concept of the premise, these embodiments having described that can also be made some replacements or modification by it, And these substitute or variant all should be considered as belonging to protection scope of the present invention.

Claims (3)

1. the method that a kind is raw material production biodiesel with algae, it is characterised in that comprise the following steps:
S1. buttress shaft algae is cultivated: with buttress shaft algae as raw material, then is seeded to cultivate with the inoculum density of ten thousand cell/mL of 300-500 Base, irradiates 20-24h/ days with red blue dual-colored LED light compensating lamp, controls intensity of illumination and control in 4000-5000LX, cultivation temperature 24-27 DEG C, cultivating 5-8 days, the density of buttress shaft algae reaches concentration and reaches 70,000,000 cell/mL, collects buttress shaft frustule；Described Culture medium composed of the following components: glucose 30-40mg/L, NaNO3 1.2-1.4mg/L、KH2PO41.2-1.4mg/L, shell Polysaccharide 1.0-1.4mg/L, biogas slurry 6-8mg/L, f2 vitamin 3-4mL and sea water 1L；
S2. oils and fats extracts: after step S1 is collected buttress shaft frustule concentrate drying, uses ultrasound wave to carry out buttress shaft algae at breaking cellular wall Reason, obtains buttress shaft algae powder, and adding in buttress shaft algae powder with its mass ratio is the petroleum ether of 1:9-12, and mixing stands 3-7h, upper liquid It is oils and fats extracting solution, then by extracting solution 80-85 DEG C of distillation, i.e. can get microalgae grease；
S3. ester exchange reaction: be 1:4-6:0.02-according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst The ratio of 0.04 adds in reactor, at temperature is 55-70 DEG C, carries out esterification 90-120min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 12-15min, stratification under rotating speed is 100-150r/min, isolate Upper strata glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 70-80 DEG C, the most available Biodiesel.
The most according to claim 1 a kind of be raw material production biodiesel with algae method, it is characterised in that: institute HONGGUANG in the red blue dual-colored LED light compensating lamp stated is 1:2-3 with blue light proportioning.
The most according to claim 1 and 2 a kind of be raw material production biodiesel with algae method, its feature exists In: using ultrasound wave that buttress shaft algae is carried out broken wall treatment described in step S2, ultrasonic power is 150-200W, ultrasonic time is 35- 40min。
CN201610772842.8A 2016-08-30 2016-08-30 Method for preparing biodiesel from algae as raw material Pending CN106318606A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101649332A (en) * 2008-08-15 2010-02-17 上海高技企业发展公司 Production method of biodiesel
CN101767893A (en) * 2010-02-11 2010-07-07 清华大学 Device and method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater
CN103045352A (en) * 2011-10-17 2013-04-17 中国石油化工股份有限公司 Extraction method of microalga grease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101649332A (en) * 2008-08-15 2010-02-17 上海高技企业发展公司 Production method of biodiesel
CN101767893A (en) * 2010-02-11 2010-07-07 清华大学 Device and method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater
CN103045352A (en) * 2011-10-17 2013-04-17 中国石油化工股份有限公司 Extraction method of microalga grease

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Application publication date: 20170111