CN106190537A - A kind of processing method of microalgae biodiesel - Google Patents
A kind of processing method of microalgae biodiesel Download PDFInfo
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- CN106190537A CN106190537A CN201610772862.5A CN201610772862A CN106190537A CN 106190537 A CN106190537 A CN 106190537A CN 201610772862 A CN201610772862 A CN 201610772862A CN 106190537 A CN106190537 A CN 106190537A
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- biodiesel
- nannochloropsis oceanica
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The processing method that the invention discloses a kind of microalgae biodiesel, comprises the following steps: Nannochloropsis oceanica is seeded to culture medium, irradiates 12 14h/ days with red blue dual-colored LED light compensating lamp, cultivates 89 days, can collect Nannochloropsis oceanica cell at 25 27 DEG C；The Nannochloropsis oceanica cell ultrasound wave collected carries out broken wall treatment, again with organic solvent extraction, obtain microalgae grease, again microalgae grease, methanol and zeolite molecular sieve catalyst are added in reactor according to the ratio that mass ratio is 1:6 8:0.01 0.04 and carry out esterification, obtain esterification products, esterification products through separating, purifying, i.e. can get biodiesel again.The inventive method, with Nannochloropsis oceanica with short production cycle, widely distributed, that photosynthesis is strong, fat content is high as raw material, solves the problem that the Vegetable oil lipoprotein raw material in biodiesel exploitation exists.The most also have that preparation technology is simple, catalytic efficiency is high, grease extraction efficiency is high, product yield advantages of higher, lay a good foundation for biodiesel industrialization, scale.
Description
Technical field
The invention belongs to biomass energy technology field, the processing method of a kind of microalgae biodiesel.
Background technology
Along with the exhaustion day by day of fossil fuel, oil price persistently sharp rises, and seeking regenerative resource has become solution
Human survival and the needs of holding World Economics sustainable development, the biodiesel as novel alternative energy source is sent out in countries in the world
Exhibition is rapidly.Biodiesel refers to oil crop such as Semen sojae atricolor, Brassica campestris L, cotton, Petiolus Trachycarpi etc., the water such as Wild oil plant and engineering microalgae
Raw vegetable oil fat and animal oil, food garbage oil etc. for raw oil by replacing that ester exchange or thermochemical processes are made
The reproducibility diesel fuel of petrifaction diesel.The advantage of biodiesel is not limited only to recyclability, compared with existing diesel oil, also has
Following advantage: Cetane number is high so that combustibility is more preferable；Preferably wettability, makes injection pump, engine cylinder-body and connecting rod
Abrasion reduce, increase the service life；In biodiesel, sulfur content is low, and the discharge of sulfur dioxide and sulfide is low, reduces environment
Pollute；Biodiesel flash-point is high, is not belonging to dangerous materials, all has superiority in transport, storage, use etc..
At present, China's industrialized production biodiesel is mainly with Oleum Brassicae campestris, Oleum Gossypii semen, Chinese tallow, shinyleaf yellowhorn oil, Oleum Camelliae
It is raw material with waste oil etc..But the limits throughput of these raw materials, the biodiesel of production can only meet current vehicle demand for fuel
The 0.3% of amount.And a large amount of Vegetable oil lipoprotein produces biodiesel, anxiety and the crops price of land resource will certainly be caused
Rise, especially in the case of China has a large population and a few land, these are particularly problematic.In this case, microalgae biodiesel
The concern of more and more people has been attracted with the advantage of its uniqueness.Microalgae, as a kind of important biodiesel raw material, is planted with oil plant
Thing is compared, and has that distribution is wide, Biomass high, photosynthetic efficiency is high, adaptive capacity to environment is strong, growth cycle is short, fat content is high, becomes
The significant advantages such as this lowest and environmental friendliness.
In recent years, research prepared by relevant biodiesel is concentrated mainly on the aspect such as woody oleiferous plants and disposed waste oil, from
Microalgae extracts oils and fats little for the research report preparing biodiesel.Wherein, biodiesel one is prepared with microalgae for raw material
As comprise the following steps that: Strain selection, pilot scale culture, extraction oils and fats, esterification prepare biodiesel coarse product, warp
Cross refine and i.e. can get biodiesel.The microalgae kind selecting fat content high is to prepare the key of biodiesel, cultivates simultaneously
Base and envirment factor also have important impact to the fat content of microalgae.Therefore, how to improve the nutritional labeling of culture medium and change
The changing environment factor is one of approach improving algae oils and fats productivity.Although improving microalgae fat content can be that biodiesel is laid well
Basis, but the production technology of biodiesel is most important to its yield effect.The production technology of existing biodiesel is general
There is problems in that catalytic efficiency is low, yield of biodiesel is low, response time length, etching apparatus, complex process, energy consumption are relatively big,
Relatively costly deficiency such as grade.
Nannochloropsis oceanica (Chlorella vulgaris), belongs to Chlorophyceae, Dictyosphaeriaceae, is that a kind of marine unicellular is micro-
The features such as algae, has easily cultivation, breeding rapidly, nutritious, fat content reaches 20%, mainly contains C in lubricant component18、
C16The fatty glyceride of carbochain, close with the biodiesel fuel component extracted from vegetable oil, it is that a class has potentiality to be exploited
Bioenergy material.
Summary of the invention
The present invention is directed to Vegetable oil lipoprotein limits throughput and the problem of existing biodiesel oil preparing process existence, it is provided that Yi Zhongwei
The processing method of algae biodiesel.The inventive method has that raw material sources are extensive, catalytic efficiency is high, it is low to consume energy, biodiesel is produced
The advantages such as rate is high, preparation technology is simple, lay a good foundation for biodiesel industrialization, scale, can effectively alleviate energy
Source crises problem.
In order to realize object above, the present invention is to be achieved through the following technical solutions:
The processing method of a kind of microalgae biodiesel, comprises the following steps:
S1. Nannochloropsis oceanica is cultivated: with Nannochloropsis oceanica as raw material, then inoculates with the inoculum density of ten thousand cell/mL of 600-800
To culture medium, irradiate 12-14h/ days with red blue dual-colored LED light compensating lamp, control intensity of illumination in 2500-3000LX, cultivation temperature
Controlling at 25-27 DEG C, cultivate 8-9 days, the density of Nannochloropsis oceanica reaches concentration and reaches 90,000,000 cell/mL, collects micro-green ball
Frustule；Described culture medium is composed of the following components: glucose 20-25mg/L, NaNO3 1.8-2.6mg/L、KH2PO41.5-
1.8mg/L, chitosan 0.7-1.1mg/L, biogas slurry 5-7mg/L, f2 vitamin 2-4mL and sea water 1L；
S2. oils and fats extracts: step S1 is collected Nannochloropsis oceanica cell concentration after drying, uses ultrasound wave to enter Nannochloropsis oceanica
Row broken wall treatment, obtains Nannochloropsis oceanica powder, and adding in Nannochloropsis oceanica powder with its mass ratio is the petroleum ether of 1:5-8, and mixing stands
4-6h, upper liquid is oils and fats extracting solution, then by extracting solution 85-90 DEG C of distillation, i.e. can get microalgae grease；
S3. ester exchange reaction: be 1:6-8 according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst:
The ratio of 0.01-0.04 adds in reactor, at temperature is 45-55 DEG C, carries out esterification 72-78min, obtains esterification and produces
Thing；
S4. standing separation；By esterification products centrifugal 12-18min, stratification under rotating speed is 250-400r/min, divide
Separating out upper strata glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 80-85 DEG C,
Obtain biodiesel.
Further, the compound method of the f2 vitamin described in above step S1 is as follows: 0.5mg vitamin B12、0.5mg
Biotin, 100mL vitamin B1 and pure water 1000mL.
Further, the HONGGUANG in above-described red blue dual-colored LED light compensating lamp is 1:1-4 with blue light proportioning, preferably joins
Ratio is 1:3.
Further, using ultrasound wave that Nannochloropsis oceanica is carried out broken wall treatment described in above step S2, ultrasonic power is
200-250W, ultrasonic time are 55-60min.
Compared with prior art, the beneficial effect that the present invention obtains:
1, the present invention prepares biodiesel with Nannochloropsis oceanica for raw material, can effectively alleviate current energy crisis and environment is dirty
Dye problem, Nannochloropsis oceanica has that distribution is wide, Biomass high, photosynthetic efficiency is high, adaptive capacity to environment is strong, growth cycle is short, oils and fats
The significant advantages such as content height, low cost and environmental friendliness, it is possible to solve the Vegetable oil lipoprotein insufficient raw material in biodiesel exploitation, one-tenth
This crosses the problems such as height.
2, the present invention adds in the medium chitosan and biogas slurry, containing nutrition such as substantial amounts of trace element, Organic substances
Composition, can accelerate microalgae speed of production and improve the oils and fats productivity of microalgae.
3, the inventive method is by using red blue dual-colored LED light compensating lamp to change illumination condition, is possible not only to improve micro-green ball
The photosynthesis of algae, accelerates Nannochloropsis oceanica and produces and improve yield, can also improve the oils and fats productivity of Nannochloropsis oceanica, with often simultaneously
Electric filament lamp, fluorescent lamp or monochromatic LED lamp compare, the oils and fats productivity of Nannochloropsis oceanica improves about 5%.
4, the inventive method uses supersonic wave wall breaking method, and ultrasound wave can produce unique mechanical vibration to microalgae cell
Effect and cavitation, advantages such as having that sporoderm-broken rate is high, process the time period, environmentally safe, power consumption are low, improve oils and fats
Extraction ratio.
5, the inventive method uses zeolite molecular sieve as catalyst, possesses that reaction interface is big, surface area big, resistance to mass tranfer
Little, reaction condition is gentle, active height, pollution-free, the not feature such as etching apparatus, improve catalytic efficiency, shorten esterification
Time, catalytic effect is notable, improves the yield of biodiesel.
6, the inventive method have that preparation technology is simple, catalytic efficiency is high, grease extraction efficiency is high, biodiesel production rate is high,
Product purity is high, raw material sources extensively, the advantage such as low cost, power consumption be low, there is good society, ecology and economic benefit, for
Microalgae biodiesel industrialization, scale are laid a good foundation.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but is not limited to protection scope of the present invention.
Embodiment 1
The preparation of Nannochloropsis oceanica culture medium: glucose 20mg/L, NaNO3 2.6mg/L、KH2PO41.8mg/L, chitosan
0.7mg/L, biogas slurry 6mg/L, f2 vitamin 2mL and sea water 1L；
The processing method of a kind of microalgae biodiesel, comprises the following steps:
S1. Nannochloropsis oceanica is cultivated: with Nannochloropsis oceanica as raw material, then is seeded to training with the inoculum density of 8,000,000 cell/mL
Supporting base, irradiate 14h/ days with red blue dual-colored LED light compensating lamp, in LED light supplement lamp, HONGGUANG and blue light proportioning are 1:1, control illumination strong
Degree at 2500LX, cultivation temperature controls at 25 DEG C, cultivates 9 days, the density of Nannochloropsis oceanica reach concentration reach 90,000,000 cells/
ML, collects Nannochloropsis oceanica cell；
S2. oils and fats extracts: step S1 is collected Nannochloropsis oceanica cell concentration after drying, uses ultrasound wave to enter Nannochloropsis oceanica
Row broken wall treatment, control ultrasonic power is 200W, and ultrasonic reaction 55min obtains Nannochloropsis oceanica powder, in Nannochloropsis oceanica powder add with
Its mass ratio is the petroleum ether of 1:5, and mixing stands 4h, and upper liquid is oils and fats extracting solution, then by extracting solution 90 DEG C of distillations, i.e.
Available microalgae grease；
S3. ester exchange reaction: be 1:6:0.04's according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst
Ratio adds in reactor, at temperature is 45 DEG C, carries out esterification 78min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 18min, stratification under rotating speed is 250r/min, isolate upper strata
Glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 80 DEG C,
To biodiesel.
Embodiment 2
The preparation of Nannochloropsis oceanica culture medium: glucose 25mg/L, NaNO3 1.8mg/L、KH2PO41.6mg/L, chitosan
0.9mg/L, biogas slurry 5mg/L, f2 vitamin 3mL and sea water 1L；
The processing method of a kind of microalgae biodiesel, comprises the following steps:
S1. Nannochloropsis oceanica is cultivated: with Nannochloropsis oceanica as raw material, then is seeded to training with the inoculum density of 7,000,000 cell/mL
Supporting base, irradiate 13h/ days with red blue dual-colored LED light compensating lamp, in LED light supplement lamp, HONGGUANG and blue light proportioning are 1:4, control illumination strong
Degree at 3000LX, cultivation temperature controls at 26 DEG C, cultivates 9 days, the density of Nannochloropsis oceanica reach concentration reach 90,000,000 cells/
ML, collects Nannochloropsis oceanica cell；
S2. oils and fats extracts: step S1 is collected Nannochloropsis oceanica cell concentration after drying, uses ultrasound wave to enter Nannochloropsis oceanica
Row broken wall treatment, control ultrasonic power is 250W, and ultrasonic reaction 60min obtains Nannochloropsis oceanica powder, in Nannochloropsis oceanica powder add with
Its mass ratio is the petroleum ether of 1:6, and mixing stands 5h, and upper liquid is oils and fats extracting solution, then by extracting solution 85 DEG C of distillations, i.e.
Available microalgae grease；
S3. ester exchange reaction: be 1:7:0.01's according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst
Ratio adds in reactor, at temperature is 50 DEG C, carries out esterification 75min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 15min, stratification under rotating speed is 300r/min, isolate upper strata
Glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 82 DEG C,
To biodiesel.
Embodiment 3
The preparation of Nannochloropsis oceanica culture medium: glucose 22mg/L, NaNO3 2.2mg/L、KH2PO41.8mg/L, chitosan
1.1mg/L, biogas slurry 7mg/L, f2 vitamin 4mL and sea water 1L；
The processing method of a kind of microalgae biodiesel, comprises the following steps:
S1. Nannochloropsis oceanica is cultivated: be seeded to training with the inoculum density of 6,000,000 cell/mL with Nannochloropsis oceanica again for raw material
Supporting base, irradiate 12h/ days with red blue dual-colored LED light compensating lamp, in LED light supplement lamp, HONGGUANG and blue light proportioning are 1:3, control illumination strong
Degree at 2700LX, cultivation temperature controls at 27 DEG C, cultivates 8 days, the density of Nannochloropsis oceanica reach concentration reach 90,000,000 cells/
ML, collects Nannochloropsis oceanica cell；
S2. oils and fats extracts: step S1 is collected Nannochloropsis oceanica cell concentration after drying, uses ultrasound wave to enter Nannochloropsis oceanica
Row broken wall treatment, control ultrasonic power is 220W, and ultrasonic reaction 58min obtains Nannochloropsis oceanica powder, in Nannochloropsis oceanica powder add with
Its mass ratio is the petroleum ether of 1:8, and mixing stands 6h, and upper liquid is oils and fats extracting solution, then by extracting solution 88 DEG C of distillations, i.e.
Available microalgae grease；
S3. ester exchange reaction: be 1:8:0.02's according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst
Ratio adds in reactor, at temperature is 55 DEG C, carries out esterification 72min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 12min, stratification under rotating speed is 400r/min, isolate upper strata
Glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 85 DEG C,
To biodiesel.
The Nannochloropsis oceanica utilizing oils and fats extraction weight method to collect above example carries out fat content mensuration, and result is such as
Shown in table 1.Wherein oils and fats total content=(microalgae grease/Nannochloropsis oceanica powder) × 100%.
Table 1. Nannochloropsis oceanica fat content
| Oils and fats total content (%) | |
| Embodiment 1 | 23 |
| Embodiment 2 | 26 |
| Embodiment 3 | 24 |
Biodiesel prepared by above example is according to GB/T20828-2007 (diesel fuel blending biodiesel)
The performance of standard detection product, testing result is as shown in table 2.
The properties of product of the biodiesel of table 2. the present embodiment
| Density (g/cm3) | Viscosity (40 DEG C of mm2/s) | Acid number (mgKOH/g) | Flash-point (DEG C) | Cetane number | |
| Embodiment 1 | 0.83 | 4.46 | 0.52 | 185 | 53 |
| Embodiment 2 | 0.87 | 4.98 | 0.65 | 174 | 69 |
| Embodiment 3 | 0.85 | 5.24 | 0.71 | 163 | 61 |
| Standard value | 0.82-0.9 | 1.9-6.0 | ≤0.8 | ≥130℃ | ＞ 49 |
As can be seen from the above table, the indices of biodiesel prepared by the present invention is above diesel fuel blending life
The standard of thing diesel oil, good market prospects.
Above content is to combine concrete/the most made for the present invention further description, it is impossible to
Assert the present invention be embodied as be confined to these explanations.General technical staff of the technical field of the invention is come
Saying, without departing from the inventive concept of the premise, these embodiments having described that can also be made some replacements or modification by it,
And these substitute or variant all should be considered as belonging to protection scope of the present invention.
Claims (3)
1. the processing method of a microalgae biodiesel, it is characterised in that comprise the following steps:
S1. Nannochloropsis oceanica is cultivated: with Nannochloropsis oceanica as raw material, then is seeded to training with the inoculum density of ten thousand cell/mL of 600-800
Support base, irradiate 12-14h/ days with red blue dual-colored LED light compensating lamp, control intensity of illumination and control in 2500-3000LX, cultivation temperature
At 25-27 DEG C, cultivating 8-9 days, the density of Nannochloropsis oceanica reaches concentration and reaches 90,000,000 cell/mL, collects Nannochloropsis oceanica thin
Born of the same parents；Described culture medium is composed of the following components: glucose 20-25mg/L, NaNO31.8-2.6mg/L、KH2PO41.5-
1.8mg/L, chitosan 0.7-1.1mg/L, biogas slurry 5-7mg/L, f2 vitamin 2-4mL and sea water 1L；
S2. oils and fats extracts: step S1 is collected Nannochloropsis oceanica cell concentration after drying, uses ultrasound wave to break Nannochloropsis oceanica
Wall processes, and obtains Nannochloropsis oceanica powder, and adding in Nannochloropsis oceanica powder with its mass ratio is the petroleum ether of 1:5-8, and mixing stands 4-6h,
Upper liquid is oils and fats extracting solution, then by extracting solution 85-90 DEG C of distillation, i.e. can get microalgae grease；
S3. ester exchange reaction: be 1:6-8:0.01-according to mass ratio by microalgae grease, methanol and zeolite molecular sieve catalyst
The ratio of 0.04 adds in reactor, at temperature is 45-55 DEG C, carries out esterification 72-78min, obtains esterification products；
S4. standing separation；By esterification products centrifugal 12-18min, stratification under rotating speed is 250-400r/min, isolate
Upper strata glycerol, lower floor is biodiesel crude product；
S5. distilation: biodiesel crude product is added to distillation column, is distilled off unnecessary methanol at 80-85 DEG C, the most available
Biodiesel.
The processing method of a kind of microalgae biodiesel the most according to claim 1, it is characterised in that: described red indigo plant is double-colored
HONGGUANG in LED light supplement lamp and blue light proportioning are 1:1-4.
The processing method of a kind of microalgae biodiesel the most according to claim 1 and 2, it is characterised in that: described in step S2
Using ultrasound wave that Nannochloropsis oceanica is carried out broken wall treatment, ultrasonic power is 200-250W, ultrasonic time is 55-60min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115287192A (en) * | 2022-08-30 | 2022-11-04 | 华南理工大学 | Method for co-producing high-protein biomass by utilizing oil-producing microalgae for efficient photosynthetic carbon fixation and application of method |
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| CN101649332A (en) * | 2008-08-15 | 2010-02-17 | 上海高技企业发展公司 | Production method of biodiesel |
| CN101767893A (en) * | 2010-02-11 | 2010-07-07 | 清华大学 | Device and method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater |
| CN103045352A (en) * | 2011-10-17 | 2013-04-17 | 中国石油化工股份有限公司 | Extraction method of microalga grease |
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2016
- 2016-08-30 CN CN201610772862.5A patent/CN106190537A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649332A (en) * | 2008-08-15 | 2010-02-17 | 上海高技企业发展公司 | Production method of biodiesel |
| CN101767893A (en) * | 2010-02-11 | 2010-07-07 | 清华大学 | Device and method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater |
| CN103045352A (en) * | 2011-10-17 | 2013-04-17 | 中国石油化工股份有限公司 | Extraction method of microalga grease |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115287192A (en) * | 2022-08-30 | 2022-11-04 | 华南理工大学 | Method for co-producing high-protein biomass by utilizing oil-producing microalgae for efficient photosynthetic carbon fixation and application of method |
| CN115287192B (en) * | 2022-08-30 | 2024-04-19 | 华南理工大学 | Method for co-production of high-protein biomass by utilizing oil-producing microalgae to efficiently photosynthetic carbon fixation and application of method |
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Application publication date: 20161207 |