CN106315851A - Synergic denitrification technology of heterotrophic nitrification and aerobic denitrification bacteria and ammonia oxidizing bacteria - Google Patents

Synergic denitrification technology of heterotrophic nitrification and aerobic denitrification bacteria and ammonia oxidizing bacteria Download PDF

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Publication number
CN106315851A
CN106315851A CN201610835407.5A CN201610835407A CN106315851A CN 106315851 A CN106315851 A CN 106315851A CN 201610835407 A CN201610835407 A CN 201610835407A CN 106315851 A CN106315851 A CN 106315851A
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reactor device
sbr reactor
heterotrophic nitrification
nitrogen
oxidizing bacteria
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CN106315851B (en
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戴晓虎
李宁
娄竞丹
华煜
李旭曜
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Tongji University
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Tongji University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/301—Aerobic and anaerobic treatment in the same reactor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1236—Particular type of activated sludge installations
    • C02F3/1263—Sequencing batch reactors [SBR]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/302—Nitrification and denitrification treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/302—Nitrification and denitrification treatment
    • C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention discloses a synergic denitrification technology of heterotrophic nitrification and aerobic denitrification bacteria and ammonia oxidizing bacteria. The synergic denitrification technology is characterized in that organic carbon and part of ammonia nitrogen in domestic sewage are removed through the heterotrophic nitrification and aerobic denitrification bacteria, and the purpose of removing the ammonia nitrogen and the organ carbon in the domestic sewage is achieved by the aid of the advantage of promoting growth of the ammonia oxidizing bacteria through an intermediate reaction product-hydroxylamine and converting the rest of the ammonia nitrogen in the domestic sewage into nitrite nitrogen through the ammonia oxidizing bacteria. A biofilm culturing method is adopted in the technology, a granular structure that the ammonia oxidizing bacteria is wrapped through the heterotrophic nitrification and aerobic denitrification bacteria is formed to promote the synergic effect of two bacteria, denitrification reaction by additionally adding carbon source is not needed, simpleness in the process is achieved, and the organ carbon and the ammonia nitrogen in the domestic sewage are effectively removed.

Description

A kind of heterotrophic nitrification aerobic denitrifying bacterium works in coordination with denitrification process with ammonia oxidizing bacteria
Technical field
The present invention relates to heterotrophic nitrification aerobic denitrifying bacterium and work in coordination with denitrification process with ammonia oxidizing bacteria, be specifically related to a kind of base In the granule sludge structure utilizing heterotrophic nitrification aerobic denitrifying bacterium (outward) and ammonia oxidizing bacteria (AOB) (interior) to be formed in low Organic carbon and ammonia nitrogen in city domestic sewage, and the technique generating nitrite nitrogen is effectively removed under the conditions of temperature.
Background technology
City domestic sewage generally refers to the general name of all sewage discharged for maintaining resident's daily life level. The process level of city domestic sewage is directly connected to the live and work environment of urbanite.So dealing carefully with sanitary sewage Problem, reduces its destruction to natural environment, is particularly important the life and work quality improving urbanite.
Biological denitrification method Treating Municipal Sewage is used to there are some problems at present.Owing in sewage, carbon-nitrogen ratio is general relatively Low, at nitrobacteria and heterotrophic bacteria in the competition process to substrate, nitrobacteria is not preponderated, and denitrifying bacteria also needs to Organic offer electron acceptor, this is accomplished by throwing carbon source outside the denitrification stage.And nitrifying process and denitrification process need to be not Carry out under the conditions of Tong, need to arrange different path combinations.
Heterotrophic nitrification aerobic denitrifying bacterium has Heterotrophic nitrification and aerobic denitrifying ability simultaneously.Ammonia oxidizing bacteria is by ammonia The process of oxidation generation nitrite is broadly divided into 2 reactions steps, first ammonia and is oxidized to azanol under the catalysis of AMo, with After again by HAO catalysis oxidation generate nitrite.
Ammonia oxidizing bacteria (being called for short AOB) converts ammonia into nitrite nitrogen during Nitrification, it is achieved nitrosification, It is the requisite step of nitrifying process, is also its first rate-limiting step.But AOB poor growth, nutritional need is special so that This step reaction limited speed.
There are some researches show at present, azanol can promote the growth of AOB.Heterotrophic nitrification aerobic denitrifying bacterium can utilize dirt Carbon source in water also produces azanol, simultaneously as the continuous minimizing of carbon source, the growth of this bacterioid can be suppressed, the most different Support nitrification aerobic denitrifying bacteria and ammonia oxidizing bacteria in the competition in nitrogen source is preponderated by ammonia oxidizing bacteria, and heterotrophic nitrification aerobic is anti- The intermediate product azanol that nitrifier (such as Bacillus foecalis alkaligenes) is generated can promote the growth of AOB further.This invented technology with The competitive relation balancing the heterotrophic nitrification aerobic denitrifying bacterium of easy growth and breeding and ammonia oxidizing bacteria is numerous to the growth promoting AOB Grow, and remove the organic carbon in sewage and ammonia nitrogen the most simultaneously.
Summary of the invention
It is an object of the invention to provide a kind of heterotrophic nitrification aerobic denitrifying bacterium and work in coordination with denitrification process with ammonia oxidizing bacteria.Logical Cross the granule sludge utilizing heterotrophic nitrification aerobic denitrifying bacterium (outward) and ammonia oxidizing bacteria (interior) to be formed, reach to go scrubbing simultaneously Organic carbon and the purpose of ammonia nitrogen in water.
A kind of heterotrophic nitrification aerobic denitrifying bacterium that the present invention proposes and ammonia oxidizing bacteria work in coordination with denitrification process, described technique Realized by collaborative nitrogen rejection facility, described collaborative nitrogen rejection facility by intake chamber, water bath, peristaltic pump, sbr reactor device, effusion meter, Air pump, heating rod, electromagnetic valve and effluent trough, sbr reactor device is positioned at water bath, arranges heating rod, air pump in water bath Being connected to bottom sbr reactor device by effusion meter and pipeline, it is achieved the limited aeration to sbr reactor device, intake chamber is by wriggling Pump and pipeline connect sbr reactor device top, connect effluent trough by electromagnetic valve and pipeline in the middle part of sbr reactor device；In sbr reactor device It is provided with agitating device；Specifically comprise the following steps that
(1) cultivation of low temperature sbr reactor device kind mud and domestication in
Take the ammonia oxidizing bacteria (AOB) cultivated through acclimating and through the heterotrophic nitrification aerobic denitrifying bacterium of pure culture It is respectively placed in sbr reactor device, uses the form of biofilm, it is provided that stirring and limited aeration slowly, control the temperature of sbr reactor device Degree is at middle cryogenic conditions, and i.e. 15 DEG C-25 DEG C, DO is under the conditions of 0.5-1.0mg/L, and sanitary sewage water inlet and water discharging time are spaced apart 6h, control hydraulic detention time is 12h；Through 2-3 days, film forms heterotrophic nitrification aerobic denitrifying bacterium parcel ammonia oxidizing bacteria The granule sludge of AOB, i.e. completes the domestication to sbr reactor device of kind of the mud；
(2) operation of low temperature sbr reactor device in
By pending containing COD and NH4 +Sanitary sewage introduce inoculation heterotrophic nitrification aerobic denitrifying bacterium parcel ammonia oxidizing bacteria (AOB) the sbr reactor device of granule sludge processes, and the temperature controlling sbr reactor device is 15-25 DEG C, and mixing speed is 30rmp, DO are in the range of 0.5-1.0mg/L, and control hydraulic detention time is 12h, makes the organic carbon in sanitary sewage and part Ammonia nitrogen is utilized by heterotrophic nitrification aerobic denitrifying bacterium and removes, and residue mineralized nitrogen becomes nitrite nitrogen or nitrate nitrogen, nitrite nitrogen It is (60-80%) with the mass percent of nitrate nitrogen: (20-40%), finally realizes under middle cryogenic conditions, remove life dirt simultaneously Organic carbon in water and ammonia nitrogen.
In the present invention, heterotrophic nitrification aerobic denitrifying bacterium uses Bacillus foecalis alkaligenes.
The beneficial effects of the present invention is:
(1) present invention can the most effectively remove the organic carbon in sanitary sewage and ammonia nitrogen, it is not necessary to throws outside the denitrification stage Carbon source.
(2) present invention utilizes the synergism of heterotrophic nitrification aerobic denitrifying bacterium and ammonia oxidizing bacteria, improve life The treatment effeciency of sewage.
(3) nitrifying process of the present invention and denitrification process are carried out in the same apparatus, and flow process is simple, it is not necessary to arrange difference Path combination.
(4) present invention can be carried out under middle cryogenic conditions, can be as follow-up anaerobic ammonium oxidizing bacteria at middle low temperature bar The fundamental technology of sanitary sewage is processed under part.
Accompanying drawing explanation
Fig. 1 heterotrophic nitrification aerobic denitrifying bacterium works in coordination with ammonia oxidizing bacteria granule schematic diagram；Inside is ammonia oxidizing bacteria, limit The white portion of edge is heterotrophic nitrification aerobic denitrifying bacterium.
Fig. 2 heterotrophic nitrification aerobic denitrifying bacterium works in coordination with the schematic diagram of ammonia oxidizing bacteria denitrification process；
Label in figure: 1 is intake chamber, 2 is peristaltic pump, and 3 is water bath, and 4 is heating rod, and 5 is sbr reactor device, and 6 is effusion meter, 7 For air pump, 8 is electromagnetic valve, and 9 is effluent trough.
Detailed description of the invention
Combine accompanying drawing below by embodiment and further illustrate the present invention.
Embodiment 1:
Certain sbr reactor device of table 1 water inlet property analysis
Index Concentration (mg/L)
TS 700
BOD 200
TOC 160
COD 400
TN 40
Free ammonia 25
1) cultivation of sbr reactor device kind mud and domestication
The pure culture of heterotrophic nitrification aerobic denitrifying bacterium: heterotrophic nitrification aerobic denitrifying bacterium (Bacillus foecalis alkaligenes: Alcaligenes Faecalis No.4) it is saved under the conditions of-80 DEG C in the glycerite of 25%, take after cultivating 72h in 37 DEG C of shaking tables With.
The acclimating of ammonia oxidizing bacteria (AOB): take from the nitrifying groove of certain nitration denitrification reactor.
By water inlet introducing in table 1, containing Bacillus foecalis alkaligenes, (biofilm is in Non-woven fabrics with ammonia oxidizing bacteria composite particles mud Filler on) 10LSBR reactor process, control temperature in the range of 15-25 DEG C, mixing speed is that 30rmp, DO exist In the range of 0.5-1.0mg/L, pH is 7.5-8.5, and hydraulic detention time is 12h.Through 2-3 days, non-woven fabrics forms heterotrophism nitre Change the granule sludge of aerobic denitrifying bacteria parcel ammonia oxidizing bacteria AOB, i.e. complete sbr reactor device is planted the domestication of mud.
2) operation of sbr reactor device
By pending containing COD and NH4 +Sanitary sewage introduce inoculation heterotrophic nitrification aerobic denitrifying bacterium parcel ammonia oxidizing bacteria (AOB) the sbr reactor device of granule sludge processes, and the temperature controlling sbr reactor device is 15-25 DEG C, and mixing speed is 30rmp, DO are in the range of 0.5-1.0mg/L, and control hydraulic detention time is 12h, makes the organic carbon in sanitary sewage and part Ammonia nitrogen is utilized by heterotrophic nitrification aerobic denitrifying bacterium and removes, and residue mineralized nitrogen becomes nitrite nitrogen or nitrate nitrogen, nitrite nitrogen Being (60-80%) with the mass percent of nitrate nitrogen: (20-40%), the water analysis of reacted device water outlet can obtain, COD and ammonia nitrogen Clearance be more than 90%.
Embodiment 2: continuous flow reactor
Certain continuous flow reactor of table 2 water inlet property analysis
Index Concentration (mg/L)
TS 700
BOD 200
TOC 160
COD 400
TN 40
Free ammonia 25
1) cultivation of flow reactor kind mud and domestication
The pure culture of heterotrophic nitrification aerobic denitrifying bacterium: heterotrophic nitrification aerobic denitrifying bacterium (Bacillus foecalis alkaligenes: Alcaligenes Faecalis No.4) it is saved under the conditions of-80 DEG C in the glycerite of 25%, take after cultivating 72h in 37 DEG C of shaking tables With.
The acclimating of ammonia oxidizing bacteria (AOB): take from the nitrifying groove of certain nitration denitrification reactor.
Being intake by table 2, containing Bacillus foecalis alkaligenes, (biofilm is in Non-woven fabrics with ammonia oxidizing bacteria composite particles mud in introducing On filler) 10L flow reactor process, control temperature in the range of 15-25 DEG C, mixing speed is that 30rmp, DO exist In the range of 0.5-1.0mg/L, pH is 7.5-8.5, and hydraulic detention time is 12h.Through 2-3 days, non-woven fabrics forms heterotrophism nitre Change the granule sludge of aerobic denitrifying bacteria parcel ammonia oxidizing bacteria AOB, i.e. complete flow reactor is planted the domestication of mud.
2) operation of flow reactor
The 10L continuous flow reactor that above-mentioned water inlet introduces containing Bacillus foecalis alkaligenes with ammonia oxidizing bacteria composite particles mud is carried out Process, the temperature of reactor is 15-25 DEG C, mixing speed be 30rmp, DO in the range of 0.5-1.0mg/L, control hydraulic retention Time be 12h, pH be 7.5-8.5, by the sludge reflux of sedimentation tank to main reactor.Organic carbon in sanitary sewage and part Ammonia nitrogen is utilized by heterotrophic nitrification aerobic denitrifying bacterium and removes, and residue mineralized nitrogen becomes nitrite nitrogen or nitrate nitrogen, reacted device The water analysis of water outlet can obtain, and the clearance of COD and ammonia nitrogen is more than 90%.

Claims (2)

1. a heterotrophic nitrification aerobic denitrifying bacterium works in coordination with denitrification process with ammonia oxidizing bacteria, it is characterised in that described technique is passed through Collaborative nitrogen rejection facility realizes, and described collaborative nitrogen rejection facility is by intake chamber, water bath, peristaltic pump, sbr reactor device, effusion meter, air Pump, heating rod, electromagnetic valve and effluent trough, sbr reactor device is positioned at water bath, arranges heating rod in water bath, and air pump passes through Effusion meter and pipeline are connected to bottom sbr reactor device, it is achieved the limited aeration to sbr reactor device, intake chamber by peristaltic pump and Pipeline connects sbr reactor device top, connects effluent trough by electromagnetic valve and pipeline in the middle part of sbr reactor device；It is provided with in sbr reactor device Agitating device；Specifically comprise the following steps that
(1) cultivation of low temperature sbr reactor device kind mud and domestication in
Take the ammonia oxidizing bacteria (AOB) cultivated through enrichment and domestication and through the heterotrophic nitrification aerobic denitrifying of pure culture Bacterium is respectively placed in sbr reactor device, uses the form of biofilm, it is provided that stirring and limited aeration slowly, controls sbr reactor device Temperature is at middle cryogenic conditions, and i.e. 15 DEG C-25 DEG C, DO is under the conditions of 0.5-1.0mg/L, and sanitary sewage water inlet and water discharging time are spaced For 6h, control hydraulic detention time is 12h；Through 2-3 days, film forms the parcel ammoxidation of heterotrophic nitrification aerobic denitrifying bacterium thin The granule sludge of bacterium AOB, i.e. achieves the domestication of kind of mud in sbr reactor device；
(2) operation of low temperature sbr reactor device in
By pending containing COD and NH4 +Sanitary sewage introduce inoculation heterotrophic nitrification aerobic denitrifying bacterium parcel ammonia oxidizing bacteria (AOB) the sbr reactor device of granule sludge processes, and the temperature controlling sbr reactor device is 15-25 DEG C, and mixing speed is 30rmp, DO are in the range of 0.5-1.0mg/L, and control hydraulic detention time is 12h, makes the organic carbon in sanitary sewage and part Ammonia nitrogen is utilized by heterotrophic nitrification aerobic denitrifying bacterium and removes, and residue mineralized nitrogen becomes nitrite nitrogen or nitrate nitrogen, nitrite nitrogen It is (60-80%) with the mass percent of nitrate nitrogen: (20-40%), finally realizes under middle cryogenic conditions, remove life dirt simultaneously Organic carbon in water and ammonia nitrogen.
Heterotrophic nitrification aerobic denitrifying bacterium the most according to claim 1 and ammonia oxidizing bacteria work in coordination with denitrification process, its feature It is that heterotrophic nitrification aerobic denitrifying bacterium uses Bacillus foecalis alkaligenes.
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109516561A (en) * 2018-12-28 2019-03-26 中国人民大学 A kind of method that town domestic sewage fast implements nitrite accumulation during heterotrophic nitrification
CN109553247A (en) * 2018-12-28 2019-04-02 中国人民大学 A method of Anammox is realized based on heterotrophic nitrification process
CN113023893A (en) * 2021-04-28 2021-06-25 武汉工程大学 Denitrification process constructed by heterotrophic nitrification-aerobic denitrification bacteria and application of denitrification process in treatment of ammonia nitrogen wastewater of rare earth ore
CN113304684A (en) * 2021-06-02 2021-08-27 浙江大学 Continuous flow aerobic granular sludge reaction device and method with inner sleeve
CN113304685A (en) * 2021-06-02 2021-08-27 浙江大学 Continuous flow aerobic granular sludge reaction device with stirring paddle internal member and method
CN114249423A (en) * 2021-12-24 2022-03-29 成都理工大学 Low-carbon-nitrogen-ratio high-ammonia-nitrogen wastewater treatment device and method

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CN101665777A (en) * 2009-09-18 2010-03-10 北京工商大学 Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof
CN102241459A (en) * 2011-06-01 2011-11-16 北京大学 Method for reinforcing adsorption-biodegradation (AB) denitrification technology by use of heterotrophic nitrification-aerobic denitrification bacteria
CN105110581A (en) * 2015-10-08 2015-12-02 南京大学 Biological denitrification process of organic nitrogen waste water

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Publication number Priority date Publication date Assignee Title
CN1584014A (en) * 2004-06-04 2005-02-23 中国人民解放军军事医学科学院卫生学环境医学研究所 Acinetobacter for denitrification and decomposing ammonia in wastewater
CN101665777A (en) * 2009-09-18 2010-03-10 北京工商大学 Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof
CN102241459A (en) * 2011-06-01 2011-11-16 北京大学 Method for reinforcing adsorption-biodegradation (AB) denitrification technology by use of heterotrophic nitrification-aerobic denitrification bacteria
CN105110581A (en) * 2015-10-08 2015-12-02 南京大学 Biological denitrification process of organic nitrogen waste water

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109516561A (en) * 2018-12-28 2019-03-26 中国人民大学 A kind of method that town domestic sewage fast implements nitrite accumulation during heterotrophic nitrification
CN109553247A (en) * 2018-12-28 2019-04-02 中国人民大学 A method of Anammox is realized based on heterotrophic nitrification process
CN109553247B (en) * 2018-12-28 2021-08-31 中国人民大学 Method for realizing anaerobic ammonia oxidation based on heterotrophic nitrification process
CN113023893A (en) * 2021-04-28 2021-06-25 武汉工程大学 Denitrification process constructed by heterotrophic nitrification-aerobic denitrification bacteria and application of denitrification process in treatment of ammonia nitrogen wastewater of rare earth ore
CN113304684A (en) * 2021-06-02 2021-08-27 浙江大学 Continuous flow aerobic granular sludge reaction device and method with inner sleeve
CN113304685A (en) * 2021-06-02 2021-08-27 浙江大学 Continuous flow aerobic granular sludge reaction device with stirring paddle internal member and method
CN114249423A (en) * 2021-12-24 2022-03-29 成都理工大学 Low-carbon-nitrogen-ratio high-ammonia-nitrogen wastewater treatment device and method

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