CN106309368A - Composite bioactive factor liposome and preparation method thereof - Google Patents
Composite bioactive factor liposome and preparation method thereof Download PDFInfo
- Publication number
- CN106309368A CN106309368A CN201510354513.7A CN201510354513A CN106309368A CN 106309368 A CN106309368 A CN 106309368A CN 201510354513 A CN201510354513 A CN 201510354513A CN 106309368 A CN106309368 A CN 106309368A
- Authority
- CN
- China
- Prior art keywords
- liposome
- composite bio
- active factor
- factor
- active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicinal Preparation (AREA)
Abstract
The invention relates to the field of biology, and especially relates to a composite bioactive factor liposome and a preparation method thereof. The bioactivity and stability of the composite bioactive factor are both improved, and the preservation time of the composite bioactive factor is prolonged. The preparation method comprises the following steps: (1) slowly adding 1 to 5 parts of phospholipid dissolved in an organic solvent and 0.02 to 0.2 part of cholesterol solution into a solution, which contains 0.005 to 0.01 part of composite bioactive factor, and continuously stirring the solution to obtain composite bioactive factor coarse liposome; and (2) subjecting the obtained composite bioactive factor coarse liposome to ultrasonic granulation to obtain composite bioactive factor liposome.
Description
Technical field
The present invention relates to biological field, particularly relate to composite bio-active factor liposome and system thereof
Preparation Method.
Background technology
When cell is cultivated in vitro can with the multiple biological activity of paracrine, autocrine or endocrine because of
Son, for regulating growth and the differentiation of cell, promotes the Nutrition and Metabolism of cell.Research finds:
Bioactie agent can promote the Nutrition and Metabolism of Skin Cell, in beauty and skin care and health care
Aspect has broad application prospects, such as, and the BFGF in organizational project bilayer skin culture fluid
(Chinese: basic fibroblast growth factor, English: basic fibroblast growth
Factor) there is anti-aging, whitening crease-resistant, pre-, speckle dispelling, sun-proof with after-sun, prevention powder
Sting, go cicatrix etc. to act on, and EGF (Chinese: epidermal growth factor, English: epidermal
Growth factor) there is effect of reparation, crease-resistant, defying age, desalination mottle and moisturizing etc.,
Compared with common cosmetics, bioactive ingredients therein can directly participate in skin metabolism,
It is free from side effects, it is possible to inherently solve skin problem.
But, the biological activity of the composite bio-active factor directly affects the composite bio-active factor
Application.Liposome is the self-assembly of lipid molecule (lipoid), is a kind of by one or more
The structure of micro-aqueous phase it is coated with in the middle of double-layer of lipoid.In the forming process of liposome, hydrophilic head
Portion forms the surfaces externally and internally layer of film, and oil loving afterbody is in the centre of film.This of liposome
Plant structure and make it have certain envelop rate, it is possible to make the biological activity of the composite bio-active factor
It is maintained at higher level.
Such as, the liposome recombined human that the patent of Publication No. CN 101444619A provides
The preparation method of ciliary neurotrophic factor includes: the first step: monosialyl tetrahexose is neural
Joint glycosides fat GMI, PHOSPHATIDYL ETHANOLAMINE and the mixture of cholesterol, with methanol and the mixing of chloroform
Solvent dissolves；Second step: above-mentioned mixed liquor is added in Rotary Evaporators, rotary evaporation, wave
Ethanol, forms uniform lipid film to the greatest extent；3rd step: add recombination human ciliary neurotrophy factor
Phosphate solution, obtain the thick suspension of liposome；4th step: suspension thick to liposome surpasses
Sonication, then microporous filter membrane granulate in addition, obtain recombination human ciliary neurotrophy factor liposome.
The recombination human ciliary neurotrophy factor liposome obtained by above-mentioned preparation method is only comprised
Single bioactie agent, nutritional labeling is not comprehensive, limits the application of liposome.Example again
As, the ethanol injection-dynamic high-pressure of the patent offer of Publication No. CN 103637989A is micro-to be penetrated
Stream is prepared the method for tea polyphenol nano lipidosome and is included: the first step: by lecithin, cholesterol,
Tween 80 and the mixture of tea polyphenols, use a large amount of anhydrous alcohol solution；Second step: by above-mentioned
Mixed liquor is slowly injected in phosphate solution, forms emulsion；3rd: by thick for liposome suspension
Vacuum rotating removes dehydrated alcohol, obtains the thick suspension of liposome；4th step: suspension thick to liposome
Dynamic microjet at 110MPa processes once, obtains nanometer liposome.In this preparation method
Later stage is processed and causes trouble by a large amount of dehydrated alcohol of middle employing, and granulate process is under high pressure carried out
It is unfavorable for keeping biological activity.
Summary of the invention
Present invention is primarily targeted at, it is provided that composite bio-active factor liposome and preparation thereof
Method, it is possible to increase the biological activity of the composite bio-active factor and stability, and can extend
The holding time of the composite bio-active factor.
For reaching above-mentioned purpose, the present invention adopts the following technical scheme that
On the one hand, the embodiment of the present invention provides the preparation side of composite bio-active factor liposome
Method, including:
Step 1) by organic solvent dissolve 1-5 part phospholipid and 0.02-0.2 part cholesterol solution
It is slowly injected in the solution containing 0.005-0.01 part composite bio-active factor, continuously stirred,
Obtain the thick liposome of the composite bio-active factor；
Step 2) the composite bio-active factor thick lipide supersonic granulate obtained is answered
Close bioactie agent liposome.
Preferably, described organic solvent is ethanol or the ether of 5-10 part.
Wherein, described ultrasonic granulate is particularly as follows: under conditions of power is 10-30W, ultrasonic
1s stops 1s alternate cycles 2-5min.
Optionally, step 2) also include afterwards: by described composite bio-active factor liposome
Lyophilization, it is thus achieved that composite bio-active factor liposome lyophilized powder.
Preferably, step 1) also include: add in described composite bio-active factor solutions
Freeze drying protectant.
It is further preferred that step 2) also include: to described compound after ultrasonic granulate completes
Bioactie agent liposome adds freeze drying protectant.
Optionally, described freeze drying protectant is sucrose or the lactose of 5-10 part.
On the other hand, the embodiment of the present invention provides composite bio-active factor liposome, described multiple
Close bioactie agent liposome to be prepared by preparation method described above.
Preferably, the described composite bio-active factor includes at least: hEGF
HEGF, VEGF VEGF, fibroblast growth factor FGF, conversion life
The long factor-β 1TGF-β 1, transforminggrowthfactor-β2 TGF-β 2, para-insulin one increase because of
Sub-IGF-1, No. two growth factors IGF-2 of para-insulin.
Wherein, the envelop rate of described composite bio-active factor liposome is 50%-60%, particle diameter
Distribution is 50-100nm.
Composite bio-active factor liposome that the embodiment of the present invention provides and preparation method thereof,
Step 1) in, lipoprotein solution is slowly injected in composite bio-active factor solutions, it is possible to formed
The thick liposome of the composite bio-active factor that particle diameter is less, can ensure composite bio-active because of
On the premise of sub-greater activity, improve the envelop rate of the composite bio-active factor, then by ultrasonic
Granulate can obtain the compound bio work that particle diameter is 50-100nm good absorbing effect, good stability
Sex factor liposome, the method preparation gentleness, technique is simple, the gained composite bio-active factor
The activity of the bioactie agent contained by liposome can keep 70-80%, and envelop rate reaches
50%-60%, and the holding time can be extended.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below
In describing embodiment, the required accompanying drawing used is briefly described, it should be apparent that under,
Accompanying drawing during face describes is only some embodiments of the present invention, for ordinary skill people
From the point of view of Yuan, on the premise of not paying creative work, it is also possible to obtain it according to these accompanying drawings
Its accompanying drawing.
The preparation method of the composite bio-active factor liposome that Fig. 1 provides for the embodiment of the present invention
Flow chart；
Fig. 2 is to utilize gene expression ways to detect the composite bio-active factor in the embodiment of the present invention
Liposome and the bioactive testing result schematic diagram of raw material.
Detailed description of the invention
Now will be provided in detail the reference of embodiment of the present invention, one or more example describes
In hereafter.There is provided each example as explanation the unrestricted present invention.It practice, to this area
For technical staff, it is obvious that the present invention can be carried out numerous modifications and variations and
Without departing substantially from the scope of the present invention or spirit.Such as, illustrate as the part of an embodiment
Or the feature described may be used for, in another embodiment, producing further embodiment party
Formula.Therefore, based on the embodiment in the present invention, those of ordinary skill in the art are not making
The every other embodiment obtained under creative work premise, broadly falls into the model of present invention protection
Enclose.
Material involved by the embodiment of the present invention all can pass through commercial sources or pass through applicant
Obtain.
On the one hand, the embodiment of the present invention provides the preparation side of composite bio-active factor liposome
Method, including:
Step 1) by organic solvent dissolve 1-5 part phospholipid and 0.02-0.2 part cholesterol solution
It is slowly injected in the solution of the composite bio-active factor containing 0.005-0.01 part, continuously stirred,
Obtain the thick liposome of the composite bio-active factor；
Step 2) the composite bio-active factor thick lipide supersonic granulate obtained is answered
Close bioactie agent liposome.
Wherein, 1-5 part phospholipid organic solvent dissolved and 0.02-0.2 part cholesterol solution note
For lipoprotein solution, the lipoprotein solution obtained is slowly injected in the solution of the composite bio-active factor,
This operation can shorten lipoprotein solution jitter time in described composite bio-active factor solutions,
The particle diameter of the thick liposome of the gained composite bio-active factor is relatively small, and then improves final acquisition
The envelop rate of composite bio-active factor liposome and stability.
The preparation method of the composite bio-active factor liposome that the embodiment of the present invention provides, in step
Rapid 1) in, lipoprotein solution is slowly injected in composite bio-active factor solutions, it is possible to form grain
The thick liposome of the composite bio-active factor that footpath is less, can ensure the composite bio-active factor
On the premise of greater activity, improve the envelop rate of the composite bio-active factor, then by ultrasonic whole
Grain can obtain the composite bio-active that particle diameter is 50-100nm good absorbing effect, good stability
Factor liposome, the method preparation gentleness, technique is simple, gained composite bio-active factor fat
The activity of the bioactie agent contained by plastid can keep 70-80%, and envelop rate reaches
50%-60%, and the holding time can be extended.
Wherein, step 1) in the kind of organic solvent do not limit, for example, it is possible to be methanol,
Described 1-5 part phospholipid and 0.02-0.2 part cholesterol dissolubility are preferably appointed by chloroform, ethanol etc.
What solvent, however, it is contemplated that the biology of composite bio-active factor liposome that the later stage is obtained
Application and environment friendly, it is preferred that described organic solvent is ethanol or the second of 5-10 part
Ether.
Here, the acquisition to composite bio-active factor solutions does not limits, composite bio-active
Factor solutions can obtain various bioactie agents by known channel, then carry out proportioning and obtain
, the most also can purify after concentration by organization engineering skin conditioned medium is carried out
Obtain.
It should be noted that granulate has multiple implementation, for example, it is possible to be high pressure homogenize
Machine granulate, dynamic high-pressure microjet granulate etc., in order to keep the biology of the composite bio-active factor
Activity, the embodiment of the present invention uses ultrasonic granulate, it is to avoid high pressure homogenizer granulate is with the highest
Environment under high pressure during pressure microjet granulate keeps unfavourable defect to biological activity.But,
Ultrasonic procedure is high releasable process, in order to keep described composite bio-active to the full extent
The biological activity of the factor, can arrange the ultrasound condition of gentleness.
Preferably, described ultrasonic granulate is particularly as follows: under conditions of power is 10-30W, surpass
Sound 1s stops 1s alternate cycles 2-5min.
Described ultrasonic 1s stops the heat release during 1s avoids continual ultrasonic, it is possible to subtract
Few bioactive harmful effect to the described composite bio-active factor.
In order to extend the holding time of described composite bio-active factor liposome further, preferably
, step 2) also include afterwards: by described composite bio-active factor liposome lyophilization,
Obtain composite bio-active factor liposome lyophilized powder.
Described lyophilization refers to be chilled to below freezing by material so that water is changed into ice,
Under vacuum, ice is directly translated into steam and the drying means that removes, and lyophilization is applicable to temperature-sensitive thing
Matter, such as, has bioactive material etc., can keep the steady of material by lyophilization
Finished product after qualitative and biological activity, and lyophilization is cellular, has the redissolution of excellence
Property, it is possible to recover rapidly the organizational structure of material.
Shown in freezing dry process table specific as follows 1, it is divided into three phases: prefreezing section,
Secondary drying stage and redrying stage, strictly to control pre-freezing temperature (generally in the pre-freeze stage
Several years lower than the eutectic point of pre-freeze material).If pre-freezing temperature is the lowest, then it may happen that
Can not be sufficiently frozen, foaming can be expanded when evacuation distils；If pre-freezing temperature is the lowest, no
Only can increase unnecessary energy expenditure, and for some bioactive substance, can be reduced it
Active conservation rate after lyophilizing.In the stage that the primary drying stage distils the most under vacuo, for protecting
The distillation of card ice, should open heating system, is continuing to supply the heat needed for ice distillation；Secondary is done
The moisture that the dry stage is removed is for combining moisture, and now the water vapor pressure of the surface of solids is in different journeys
The reduction of degree, rate of drying is decreased obviously.On the premise of ensureing product quality, in this stage
Planted agent properly increases temperature, is beneficial to the evaporation of moisture.
Table 1
In freezing dry process, inevitably cause the degeneration of bioactive substance, gathering,
The active force maintaining the structure of described bioactive substance is destroyed, bioactive substance
The change of structure can cause the forfeiture of its activity, in order to keep described multiple in freezing dry process
Close the biological activity of bioactie agent, frozen-dried protective can be added in freezing dry process
Agent, described freeze drying protectant has the biological function of anti-freezing, anti-dehydrating, it is possible to maintain institute
State the organizational structure of bioactive substance, it is to avoid the forfeiture of activity occurs.
Preferably, step 1) also include: add in described composite bio-active factor solutions
Freeze drying protectant.
Or, step 2) also include: to described composite bio-active after ultrasonic granulate completes
Factor liposome adds freeze drying protectant.
The kind of described freeze drying protectant is not limited, for example, it is possible to be monosaccharide and disaccharide,
Oligosaccharide, polyhydric alcohol etc., in multiple freeze drying protectant, find the guarantor of disaccharidase class by research
Protect effect and be better than other freeze drying protectants, it is possible to keep the composite bio-active factor to the full extent
Biological activity after lipidosome freeze-dried.
Preferably, described freeze drying protectant is sucrose or the lactose of 5-10 part.
We are by multiple in gene expression ways detection gained composite bio-active factor liposome
Close the biological activity of bioactie agent, i.e. according to the Antioxidant Indexes of the composite bio-active factor
CAT, GSH, Cu-SOD, Mn-SOD detect in described composite bio-active factor liposome
The biological activity of the composite bio-active factor.Testing result is as in figure 2 it is shown, according to the inspection of Fig. 2
Survey result understands, and the biological activity of gained composite bio-active factor liposome and compound bio are lived
Sex factor solution is compared, and maintains the biological activity of 70%-80%.
Embodiment
The following examples are used for the present invention is described, are not intended to limit the scope of the present invention.With
Lower embodiment is only carried out as a example by the composite bio-active factor solutions that raw materials quality mark is 1%
Illustrating, time actually used, the concentration of composite bio-active factor solutions is not to the object of the invention
Realization constitute impact.
Embodiment 1
Dissolve 1 part of soybean lecithin with 5 parts of ethanol and 0.02 part of cholesterol obtains lipoprotein solution, will
The lipoprotein solution obtained is slowly injected into containing in the solution of the composite bio-active factor of 0.008 part,
Continuously stirred, it is thus achieved that the thick liposome of the composite bio-active factor；
By the gained thick liposome of the composite bio-active factor, (ultrasound condition is: power through ultrasonic
1s alternately 2min is stopped for 10W, ultrasonic 1s) after granulate, add freeze drying protectant (5
Part sucrose) lyophilization, make composite bio-active factor liposome lyophilized powder.
Conclusion: the envelop rate of gained composite bio-active factor liposome is 50%, particle diameter is
50nm, keeps the work of the bioactie agent of more than 70% in composite bio-active factor solutions
Property, predominantly EGF, BFGF, VEGF, IGF1, IGF2, TGF-B1, TGF-B2,
PDGF and KGF, after preserving 1 year at 2-8 DEG C, envelop rate is 30%, compound bio
Active factors keeps the activity of more than 70%.
Embodiment 2
Lipoprotein solution is obtained with 5 parts of soybean lecithins of 10 parts of ether dissolutions and 0.2 part of cholesterol, will
The lipoprotein solution obtained is slowly injected into containing in the solution of the composite bio-active factor of 0.01 part, holds
Continuous stirring, it is thus achieved that the thick liposome of the composite bio-active factor；
By the gained thick liposome of the composite bio-active factor, (ultrasound condition is: power through ultrasonic
1s alternately 5min is stopped for 30W, ultrasonic 1s) after granulate, add freeze drying protectant (10
Part lactose) lyophilization, make composite bio-active factor liposome lyophilized powder.
Conclusion: the envelop rate of gained composite bio-active factor liposome is 60%, keeps compound
The activity of the bioactie agent of more than 80% in bioactie agent solution, predominantly EGF,
BFGF, VEGF, IGF1, IGF2, TGF-B1, TGF-B2, PDGF and KGF,
After preserving 1 year at 2-8 DEG C, envelop rate is 50%, and the composite bio-active factor keeps 72%
Above activity.
Embodiment 3
Dissolve 3 parts of soybean lecithins with 8 parts of ethanol and 0.1 part of cholesterol obtains lipoprotein solution, will
The lipoprotein solution obtained be slowly injected into be dissolved with freeze drying protectant (8 portions of sucrose) containing 0.005
In the solution of the composite bio-active factor of part, continuously stirred, it is thus achieved that the composite bio-active factor
Thick liposome；
By the gained thick liposome of the composite bio-active factor, (ultrasound condition is: power through ultrasonic
1s alternately 3min is stopped for 20W, ultrasonic 1s) carry out lyophilization after granulate, make
Composite bio-active factor liposome lyophilized powder.
Conclusion: the envelop rate of gained composite bio-active factor liposome is 55%, keeps compound
The activity of the bioactie agent of more than 75% in bioactie agent solution, predominantly EGF,
BFGF, VEGF, IGF1, IGF2, TGF-B1, TGF-B2, PDGF and KGF,
After preserving 1 year at 2-8 DEG C, envelop rate is 50%, and the composite bio-active factor keeps 65%
Above activity.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is also
Being not limited to this, any those familiar with the art is at the technology model that the invention discloses
In enclosing, change can be readily occurred in or replace, all should contain within protection scope of the present invention.
Therefore, protection scope of the present invention should be as the criterion with described scope of the claims.
Claims (10)
1. the preparation method of composite bio-active factor liposome, it is characterised in that including:
Step 1) 1-5 part phospholipid and 0.02-0.2 part cholesterol solution of organic solvent dissolving are slowly noted
Enter in the solution of the composite bio-active factor containing 0.005-0.01 part, continuously stirred, it is thus achieved that compound
The thick liposome of bioactie agent；
Step 2) obtain being combined life by the composite bio-active factor thick lipide supersonic granulate obtained
Thing active factors liposome.
Method the most according to claim 1, it is characterised in that described organic solvent is 5-10
The ethanol of part or ether.
Method the most according to claim 1, it is characterised in that described ultrasonic granulate particularly as follows:
Under conditions of power is 10-30W, ultrasonic 1s stops 1s alternate cycles 2-5min.
Method the most according to claim 1, it is characterised in that step 2) also include afterwards:
By described composite bio-active factor liposome lyophilization, it is thus achieved that composite bio-active factor lipid
Body lyophilized powder.
5. according to the method described in any one of claim 1-4, it is characterised in that step 1) also wrap
Include: in described composite bio-active factor solutions, add freeze drying protectant.
6. according to the method described in any one of claim 1-4, it is characterised in that step 2) also wrap
Include: after ultrasonic granulate completes, in described composite bio-active factor liposome, add frozen-dried protective
Agent.
7. according to the method described in claim 5 or 6, it is characterised in that described freeze drying protectant is
The sucrose of 5-10 part or lactose.
8. composite bio-active factor liposome, it is characterised in that described composite bio-active because of
Sub-liposome is prepared by the preparation method described in any one of claim 1-7.
Composite bio-active factor liposome the most according to claim 8, it is characterised in that
The described composite bio-active factor includes at least: hEGF hEGF, blood vessel endothelium are raw
Long factor Ⅴ EGF, fibroblast growth factor FGF, transforming growth factor-beta 1 TGF-β 1,
Transforminggrowthfactor-β2 TGF-β 2, number growth factor IGF-1 of para-insulin, para-insulin two
Number growth factor IGF-2.
Composite bio-active factor liposome the most according to claim 9, it is characterised in that
The envelop rate of described composite bio-active factor liposome is 50%-60%, and particle size distribution range is
50-100nm。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510354513.7A CN106309368A (en) | 2015-06-24 | 2015-06-24 | Composite bioactive factor liposome and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510354513.7A CN106309368A (en) | 2015-06-24 | 2015-06-24 | Composite bioactive factor liposome and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106309368A true CN106309368A (en) | 2017-01-11 |
Family
ID=57728955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510354513.7A Pending CN106309368A (en) | 2015-06-24 | 2015-06-24 | Composite bioactive factor liposome and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106309368A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112138144A (en) * | 2019-06-28 | 2020-12-29 | 杭州生物医药创新研究中心 | Liposome containing active biological factor and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160582A (en) * | 1996-12-27 | 1997-10-01 | 暨南大学生物工程研究所 | External-use composite containing cell growth factor |
| CN101199837A (en) * | 2007-11-30 | 2008-06-18 | 何荫良 | Humanized cell factor hair agent and preparing method thereof |
| CN103520007A (en) * | 2013-10-15 | 2014-01-22 | 天博医药技术（苏州）有限公司 | Skin active factor flexible nano-liposome and preparation method and application thereof |
-
2015
- 2015-06-24 CN CN201510354513.7A patent/CN106309368A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160582A (en) * | 1996-12-27 | 1997-10-01 | 暨南大学生物工程研究所 | External-use composite containing cell growth factor |
| CN101199837A (en) * | 2007-11-30 | 2008-06-18 | 何荫良 | Humanized cell factor hair agent and preparing method thereof |
| CN103520007A (en) * | 2013-10-15 | 2014-01-22 | 天博医药技术（苏州）有限公司 | Skin active factor flexible nano-liposome and preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 唐冰、唐宁宁: "人表皮活性因子柔性纳米脂质体的制备及相关性质研究", 《中国药业》 * |
| 王玮等: "VEGF脂质体的制备及其药剂学性质研究", 《河南大学学报（医学版）》 * |
| 赵应征主编: "《生物药物药剂学》", 30 June 2011, 浙江大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112138144A (en) * | 2019-06-28 | 2020-12-29 | 杭州生物医药创新研究中心 | Liposome containing active biological factor and preparation method thereof |
| CN112138144B (en) * | 2019-06-28 | 2023-02-24 | 杭州生物医药创新研究中心 | Liposome containing active biological factor and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ciurzynska et al. | Freeze-drying-application in food processing and biotechnology-a review | |
| CN101534796B (en) | Physiologically active substance-containing granular composition and method of producing the same | |
| CN103070826B (en) | A kind of Quercetin Dermatological Liposomes and lyophilized powder and its production and use thereof | |
| AU2015338684A1 (en) | Cosmetic composition containing halomonas ferment extract, and use thereof | |
| JP6026785B2 (en) | Aqueous composition | |
| EP2727580A1 (en) | Astaxanthin-containing composition, method for manufacturing same, and cosmetic | |
| CN105748416B (en) | A kind of DHA nano-emulsion freeze-dried powder and preparation method thereof | |
| CN106072623A (en) | A kind of preparation method of composite Nano emulsion | |
| CN105521483A (en) | Composite bioactive factor freeze-drying method and composite bioactive factor freeze-dried powder | |
| Liu et al. | Preparation of glycyrrhetinic acid liposomes using lyophilization monophase solution method: Preformulation, optimization, and in vitro evaluation | |
| CN113576939A (en) | Biological essence polypeptide composition, and preparation method and application of lipidosome nanocrystallization thereof | |
| CN105878047A (en) | Preparation method and application of fibroblast growth factor covering lipide calcium phosphate nanoparticles | |
| CN107405279A (en) | cosmetics containing hydrogel | |
| CN101947214A (en) | Method for preparing vitamin A microcapsules by secondary emulsification and spray drying method | |
| JPWO2008093678A1 (en) | Hyaluronidase inhibitor | |
| CN106309368A (en) | Composite bioactive factor liposome and preparation method thereof | |
| CN109966158A (en) | A kind of freeze-dried powder enabling skin whitening | |
| WO2023237004A1 (en) | Ternary freeze-dry composition and use thereof in freeze-dry formulation | |
| CN107802604A (en) | A kind of double auxiliary material latamoxef sodium for injection freeze drying powder injections | |
| CN116531265A (en) | Supermolecule micro-lipid capsule freeze-dried ball composition and preparation method and application thereof | |
| CN103110534A (en) | Combined essence of composite velvet antler polypeptide lyophilized powder and nano-scale buffering microemulsion for skin beauty and health care and preparation method of combined essence | |
| JP2008239505A (en) | Neuroblast growth promoter and neurite extender | |
| CN107789207A (en) | A kind of EGF/ vitamin e acetates cyclodextrin liposome and preparation method thereof, application method, application | |
| CN103385853A (en) | EGF liposome and preparation method thereof | |
| CN108517342A (en) | A kind of method that more algae proteolysis prepare polypeptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |
|
| RJ01 | Rejection of invention patent application after publication |