CN106303242A - The focusing system quickly of muti-spectrum imaging and method - Google Patents

The focusing system quickly of muti-spectrum imaging and method Download PDF

Info

Publication number
CN106303242A
CN106303242A CN201610688593.4A CN201610688593A CN106303242A CN 106303242 A CN106303242 A CN 106303242A CN 201610688593 A CN201610688593 A CN 201610688593A CN 106303242 A CN106303242 A CN 106303242A
Authority
CN
China
Prior art keywords
focusing
module
definition
evaluation
image
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610688593.4A
Other languages
Chinese (zh)
Inventor
胡洁
石军
黄海清
戚进
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Jiaotong University
Original Assignee
Shanghai Jiaotong University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Jiaotong University filed Critical Shanghai Jiaotong University
Priority to CN201610688593.4A priority Critical patent/CN106303242A/en
Publication of CN106303242A publication Critical patent/CN106303242A/en
Pending legal-status Critical Current

Links

Classifications

    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/60Control of cameras or camera modules
    • H04N23/67Focus control based on electronic image sensor signals

Abstract

The invention provides a kind of focusing system quickly and the method for muti-spectrum imaging, including: speed image capture module, focusing window choose module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically;Rapid image acquisition module is for the multispectral microscopic image gathered;Focusing window is automatically chosen module and is found out object-image section in multispectral microscopic image as the fine-focused area-of-interest of needs, and the positional information of focusing window is supplied to definition automatic Evaluation module;Definition automatic Evaluation module goes out definition evaluation of estimate according to the positional information calculation of the multispectral microscopic image adopted in real time and focusing window;Before search strategy judge module definition evaluation of estimate and multispectral microscopic image, the definition evaluation of estimate of 5 two field pictures judges focusing position state, and finds focus position by motor control feedback module.The present invention without Human disturbance, accuracy is high, precision high, focusing speed is fast.

Description

The focusing system quickly of muti-spectrum imaging and method
Technical field
The present invention relates to micro-image actively focusing field, in particular it relates to a kind of muti-spectrum imaging is quick main Dynamic focusing system and method.
Background technology
At present, the active focusing method at muti-spectrum imaging mainly has telemetry and image method.Telemetry is mainly Utilize the range units such as laser range finder, the distance (i.e. object distance) of detection target is measured, public in conjunction with geometric optics newton Formula, adjusts accordingly object lens height, and then realizes the rapid focus of system.The method is simple and quick, but needs additionally to increase If auxiliary bodies such as range units, add complexity and the infrastructure cost of system.Additionally, this contactless range finding is often Owing to external environmental interference factor causes serious range error, cause auto-focusing failure.Image method is mainly by adopting Collect the shade of gray of image of different out-of-focus appearances, the spectral characteristic etc. obtained and carry out Treatment Analysis, extract and focus accurately Positional information, realizes the auto-focusing of system by object lens height adjustment.Image method simple in construction, be affected by the external environment little, And neatly target interested in visual field can be carried out auto-focusing, have become as the main flow side in auto-focusing field Method.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide the most right of a kind of muti-spectrum imaging Burnt system and method.
The focusing system quickly of the muti-spectrum imaging according to present invention offer, including: speed image capture module, Focusing window chooses module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically;Its In:
Described rapid image acquisition module, for Real-time Collection multispectral microscopic image and multispectral micro-by gather Image transmitting automatically chooses module and definition automatic Evaluation module to focusing window;
Described focusing window chooses module automatically, for finding out object-image section in multispectral microscopic image as needing Want fine-focused area-of-interest, and the positional information of focusing window is supplied to definition automatic Evaluation module;
Described definition automatic Evaluation module, for according to the multispectral microscopic image adopted in real time and the position of focusing window Information calculates definition evaluation of estimate, and described definition evaluation of estimate is transferred to search strategy judge module;
Described search strategy judge module, for according to the definition evaluation of estimate received and described multispectral microscopic image Above the definition evaluation of estimate of 5 two field pictures judges that focusing position is in out-of-focus appearance or overfocus state, and according to clearly The unimodality of degree evaluation function determines the direction of motion of object lens, sends Motion to motor control feedback module;
Described motor control feedback module, adjusts with the step-length set for the Motion according to search strategy judge module Joint, to object lens height and position, finds focus position, completes actively to focus.
According to the focusing method quickly of the muti-spectrum imaging that the present invention provides, comprise the steps:
Rapid image acquisition step: Real-time Collection multispectral microscopic image;
The automatic selecting step of focusing window: find out object-image section in multispectral microscopic image the most poly-as needs Burnt area-of-interest, and record the positional information of focusing window;
Definition automatic Evaluation step: according to the multispectral microscopic image adopted in real time and the positional information calculation of focusing window Go out definition evaluation of estimate;
Search strategy judges step: according to 5 two field pictures clear before definition evaluation of estimate and described multispectral microscopic image Clear degree evaluation of estimate judges focusing position state in which, and determines the fortune of object lens according to the unimodality of sharpness evaluation function Dynamic direction, generates Motion;
Motor control feedback step: according to Motion with the step-length regulation object lens height and position set, find accurate burnt position Putting, terminate actively to focus flow process.
Preferably, the automatic selecting step of described focusing window includes: when target relativelys close to field of view center position, it is assumed that The size of image is M × N, selectedFavored area is treated as focusing window in region, treats the X of favored area, Y side at this respectively To the maximum finding projection module of differentialsMax | h (y) ' |, the most respectively withMax | h (y) ' | for CenterRegion is as focusing window.
Preferably, described definition automatic Evaluation step includes: use Gray Projection differential method to carry out computing of focusing, i.e. Using the Gray Projection first differential 1 norm average of image as sharpness evaluation function, specifically, comprise the steps:
Step A1: calculate image in focusing window respectively at projection h (x) of X and Y-direction and h (y);
Step A2: ask for projecting 1 norm h (x') and the h (y') of the first differential of h (x) and h (y) respectively;
Step A3: calculate h (x') and average P of h (y') respectivelyAvgxAnd PAvgy
Step A4: calculate PAvgxAnd PAvgyRoot-mean-square PAvg, it is definition evaluation of estimate.
Preferably, described search strategy judges that step includes: utilize ramping constraint to obtain current microcobjective height The definition evaluation of estimate of the multispectral micro-image of position, it is judged that focusing position is in out-of-focus appearance or overfocus state, if It is in out-of-focus appearance, then drives driven by motor object lens to move, if being in the direction near target object with the step-length set Coke-like state, then drive driven by motor object lens to move to the direction of wide object with the step-length set.
Preferably, described motor control feedback step includes:
Step B1: be adjusted object lens height and position according to the step-length set, line definition of going forward side by side evaluation of estimate calculates;
Step B2: judge whether the definition evaluation of estimate obtained reaches definition appraisal curve peak value, if then terminating stream Journey;Step B1 is performed if it is not, then return;
Wherein: microscope is adjusted to definition appraisal curve peak and is focus position.
Compared with prior art, the present invention has a following beneficial effect:
1, the present invention provides the focusing system quickly of muti-spectrum imaging and method focusing speed are fast, precision Height, good stability, and simple in construction, autgmentability is strong.
2, the present invention provides the focusing system quickly of muti-spectrum imaging and method are without manual intervention, it is possible to Independently complete focusing task in real time.
Accompanying drawing explanation
By the detailed description non-limiting example made with reference to the following drawings of reading, the further feature of the present invention, Purpose and advantage will become more apparent upon:
The theory diagram of the focusing system quickly of the muti-spectrum imaging that Fig. 1 provides for the present invention;
The hardware structure diagram of the focusing system quickly of the muti-spectrum imaging that Fig. 2 provides for the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area For personnel, without departing from the inventive concept of the premise, it is also possible to make some changes and improvements.These broadly fall into the present invention Protection domain.
The focusing system quickly of the muti-spectrum imaging according to present invention offer, including: rapid image gathers mould Block, focusing window choose module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically Five part compositions.
Rapid image acquisition module, for Real-time Collection multispectral microscopic image, and the multispectral microscopic image that will gather It is transferred to focusing window and automatically chooses module and definition automatic Evaluation module;
Focusing window chooses module automatically, participates in what sharpness evaluation function calculated for automatically finding out in picture in its entirety Image window;The Rational choice of focusing window is a key factor of focusing precision.Focusing window is chosen the biggest, and operand is more Greatly, but also may result in target background and participate in computing, affect the focusing accuracy of target.Focusing window is too small, may Lose the area-of-interest partial information of target, affect focus effects.
When target relativelys close to field of view center position, it is assumed that the size of image is M × N, selectedRegion conduct Focusing window treat favored area, respectively at the X in this region, Y-direction find projection module of differentials maximum max | h (x) ' |, max | H (y) ' |, the most respectively with max centered by | h (x) ' |, max | h (y) ' |Region is as focusing window.
Additionally, generally include two parts of target and background in image, target part is that needs are fine-focused interested Region, background is then the part non-interested allowing non-accurate focusing, therefore, also should note during focusing window is chosen The selection of meaning the window's position.
Definition automatic Evaluation module, for measuring the readability of image, reflects that be exactly to image in image procossing Acutance or perhaps a kind of sign of gradient.
During auto-focusing, image deviation focus position is the most remote, and image is the most unintelligible, the edge ladder that integral inverted mirrors Degree information is the fewest;Otherwise, closer to focus position, image is the most clear, and image integral edge gradient information is the abundantest;In accurate burnt position Putting place, image is the most clear, and the edge gradient information of image is the abundantest.The metric function that definition is evaluated is during auto-focusing It is equivalent to a kind of algorithm " detector ", comes according to its calculated definition evaluation of estimate in the search procedure of focus position Carry out the adjustment of optical texture.Therefore, the quality of sharpness evaluation function performance is the key factor determining auto-focusing effect.
Search strategy judge module, for referring to search for the side of accurate focusing position according to calculated definition evaluation of estimate Method, search strategy then determines the time of whole auto-focusing process.
Motor control feedback module, for according to set search strategy, according to calculated definition evaluation of estimate pair Object lens height and position carries out micro-positioning regulators, position after adjustment, and the definition evaluation of estimate re-starting image calculates, then same Sample, according to search strategy before, adjusts object lens height and position, again and so forth, until imaging is adjusted to definition and evaluates song Line peak, this position is focus position, and whole active focus process terminates.
Specifically, as in figure 2 it is shown, the focusing system quickly of muti-spectrum imaging includes: CCD camera, micro-thing Mirror, PC, MCU control module, light source, focusing drive mechanism, wherein MCU control module comprises microcontroller, motor drives Dynamic device, focusing drive mechanism comprises motor, ball-screw.First pass through rapid image acquisition module, through microscope imaging To CCD camera, the image collected is transferred to PC by USB interface;PC utilizes focusing window automatically to choose module with clear Clear degree automatic Evaluation module, passes judgment on the definition of the image collected, and according to search strategy judge module, calculates The step-length of focusing next step motion of motor and direction, utilize motor control feedback module, sends control instruction to micro-by serial ports Controller, microprocessor controls driving system of stepping motor is overall to be regulated towards imaging direction the most clearly, forms a closed loop The loop of regulation, continuous repetitive cycling, until the image collected meets the requirements.Simultaneously take account of the need of field luminance , PC can send instruction, the brightness of regulation light source to controller.
The feature that operand that described definition automatic Evaluation module make use of Gray Projection is little, real-time, by gray scale Projection thought is incorporated in auto-focusing algorithm, uses focusing degree of depth Atomatic focusing method based on projection differentiation.The method It is as sharpness evaluation function using the Gray Projection first differential 1 norm average of image, to realize this multi-optical spectrum imaging system Fast automatic focusing.
Further, the focusing method quickly of the muti-spectrum imaging in the present invention, comprise the steps:
Step S1: the determination of focusing window: before carrying out auto-focusing, first according to observation needs, in conjunction with present frame figure As carrying out the calculating of auto-focusing window position.
Step S2: target image definition values calculates:
Step S2.1: calculate image in focusing window respectively at projection h (x) of X and Y-direction and h (y);
Step S2.2: ask for 1 norm h (x') and the h (y') of its first differential respectively;
Step S2.3: calculate h (x') and average P of h (y') respectivelyAvgxAnd PAvgy
Step S2.4: calculate PAvgxAnd PAvgyRoot-mean-square PAvg, it is the definition evaluation of estimate of this frame.
Step S3: utilize ramping constraint, at the calculated current microcobjective height and position of step S2 Micro-image definition evaluation of estimate, is sequentially adjusted in object lens height and position, repeats step S2, until searching out microcobjective height Focus position.
Step S4: actively focus process terminates.
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned Particular implementation, those skilled in the art can make a variety of changes within the scope of the claims or revise, this not shadow Ring the flesh and blood of the present invention.In the case of not conflicting, the feature in embodiments herein and embodiment can any phase Combination mutually.

Claims (6)

1. the focusing system quickly of a muti-spectrum imaging, it is characterised in that including: speed image capture module, right Burnt window chooses module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically;Wherein:
Described rapid image acquisition module, for Real-time Collection multispectral microscopic image, and the multispectral microscopic image that will gather It is transferred to focusing window and automatically chooses module and definition automatic Evaluation module;
Described focusing window chooses module automatically, for finding out object-image section in multispectral microscopic image as needs standard The area-of-interest really focused on, and the positional information of focusing window is supplied to definition automatic Evaluation module;
Described definition automatic Evaluation module, for according to the multispectral microscopic image adopted in real time and the positional information of focusing window Calculate definition evaluation of estimate, and described definition evaluation of estimate is transferred to search strategy judge module;
Described search strategy judge module, before according to the definition evaluation of estimate received and described multispectral microscopic image The definition evaluation of estimate of 5 two field pictures judges that focusing position is in out-of-focus appearance or overfocus state, and comments according to definition The unimodality of valency function determines the direction of motion of object lens, sends Motion to motor control feedback module;
Described motor control feedback module, right with the step-length regulation set for the Motion according to search strategy judge module Object lens height and position, finds focus position, completes actively to focus.
2. the focusing method quickly of a muti-spectrum imaging, it is characterised in that comprise the steps:
Rapid image acquisition step: Real-time Collection multispectral microscopic image;
The automatic selecting step of focusing window: find out object-image section in multispectral microscopic image fine-focused as needs Area-of-interest, and record the positional information of focusing window;
Definition automatic Evaluation step: go out clearly according to the positional information calculation of the multispectral microscopic image adopted in real time and focusing window Clear degree evaluation of estimate;
Search strategy judges step: according to the definition of 5 two field pictures before definition evaluation of estimate and described multispectral microscopic image Evaluation of estimate judges focusing position state in which, and determines the motion side of object lens according to the unimodality of sharpness evaluation function To, generate Motion;
Motor control feedback step: according to Motion with the step-length regulation object lens height and position set, find focus position, knot Restraint flow process of actively focusing.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described focusing The automatic selecting step of window includes: when target relativelys close to field of view center position, it is assumed that the size of image is M × N, selectedFavored area is treated as focusing window in region, treats the X of favored area at this respectively, and Y-direction finds projection module of differentials | h (x) ' |, max | the h (y) ' | that is worth greatly max, the most respectively with max centered by | h (x) ' |, max | h (y) ' |Region is made For focusing window.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described clearly Degree automatic Evaluation step includes: use Gray Projection differential method to carry out computing of focusing, will the Gray Projection single order of image micro- Divide 1 norm average as sharpness evaluation function, specifically, comprise the steps:
Step A1: calculate image in focusing window respectively at projection h (x) of X and Y-direction and h (y);
Step A2: ask for projecting 1 norm h (x') and the h (y') of the first differential of h (x) and h (y) respectively;
Step A3: calculate h (x') and average P of h (y') respectivelyAvgxAnd PAvgy
Step A4: calculate PAvgxAnd PAvgyRoot-mean-square PAvg, it is definition evaluation of estimate.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described search Strategy judges that step includes: utilize ramping constraint to obtain the clear of multispectral micro-image at current microcobjective height and position Clear degree evaluation of estimate, it is judged that focusing position is in out-of-focus appearance or overfocus state, if the out-of-focus appearance of being in, then drives motor belt motor Animal mirror moves to the direction near target object with the step-length set, if being in overfocus state, then drives driven by motor object lens Move to the direction of wide object with the step-length set.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described motion Control feedback step to include:
Step B1: be adjusted object lens height and position according to the step-length set, line definition of going forward side by side evaluation of estimate calculates;
Step B2: judge whether the definition evaluation of estimate obtained reaches definition appraisal curve peak value, if then terminating flow process;If No, then return and perform step B1;
Wherein: microscope is adjusted to definition appraisal curve peak and is focus position.
CN201610688593.4A 2016-08-18 2016-08-18 The focusing system quickly of muti-spectrum imaging and method Pending CN106303242A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610688593.4A CN106303242A (en) 2016-08-18 2016-08-18 The focusing system quickly of muti-spectrum imaging and method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610688593.4A CN106303242A (en) 2016-08-18 2016-08-18 The focusing system quickly of muti-spectrum imaging and method

Publications (1)

Publication Number Publication Date
CN106303242A true CN106303242A (en) 2017-01-04

Family

ID=57660595

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610688593.4A Pending CN106303242A (en) 2016-08-18 2016-08-18 The focusing system quickly of muti-spectrum imaging and method

Country Status (1)

Country Link
CN (1) CN106303242A (en)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107395993A (en) * 2017-09-08 2017-11-24 北京睿智奥恒视觉科技有限公司 Full-automatic focusing method and system
CN109239900A (en) * 2018-11-07 2019-01-18 华东师范大学 A kind of full-automatic quick focusing method for the big visual field acquisition of microscopic digital image
CN109374621A (en) * 2018-11-07 2019-02-22 杭州迪英加科技有限公司 Focusing method, system and the device of slice scanner
CN109995998A (en) * 2019-01-03 2019-07-09 中国科学院生物物理研究所 A kind of auto focusing method being imaged suitable for scanning/transmission electron microscope
CN110278383A (en) * 2019-07-25 2019-09-24 浙江大华技术股份有限公司 Focus method, device and electronic equipment, storage medium
CN110488481A (en) * 2019-09-19 2019-11-22 广东工业大学 A kind of microscope focusing method, microscope and relevant device
CN111142253A (en) * 2019-12-05 2020-05-12 苏州大学 Focusing window selection method and device in microscopic image
CN111665617A (en) * 2020-06-24 2020-09-15 武汉中纪生物科技有限公司 Focusing method and system
CN112165570A (en) * 2020-08-29 2021-01-01 南京理工大学 Multi-depth target focusing method based on ghost imaging calculation
CN112230491A (en) * 2020-10-30 2021-01-15 广西代达科技有限公司 Application method of technical camera capable of automatically focusing
CN112312120A (en) * 2019-07-30 2021-02-02 深圳光启空间技术有限公司 Automatic adjusting system and method for camera focal plane
CN112887604A (en) * 2021-01-26 2021-06-01 深圳赛动生物自动化有限公司 Stem cell image acquisition method, device, system and medium
CN113176274A (en) * 2021-03-19 2021-07-27 哈工大机器人(中山)无人装备与人工智能研究院 Automatic focusing method, device and system for detecting defects of display panel
CN113852761A (en) * 2021-09-27 2021-12-28 宁波华思图科技有限公司 Automatic focusing method of intelligent digital microscope
CN114449152A (en) * 2020-10-30 2022-05-06 北京小米移动软件有限公司 Focusing method and device, electronic equipment and storage medium
CN115079372A (en) * 2022-05-05 2022-09-20 深圳安侣医学科技有限公司 Rapid focusing method of microscopic image acquisition device and microscopic image acquisition method
CN115314633A (en) * 2022-06-27 2022-11-08 中国科学院合肥物质科学研究院 Camera focusing method and device computer device and storage medium

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2610349Y (en) * 2003-04-01 2004-04-07 北京舒尔德科技发展有限公司 Multifunction micro-imaging instrument
CN101706609A (en) * 2009-11-23 2010-05-12 常州达奇信息科技有限公司 Image processing based fast automatic focusing method of microscope
CN101840055A (en) * 2010-05-28 2010-09-22 浙江工业大学 Video auto-focusing system based on embedded media processor
CN102998787A (en) * 2012-07-24 2013-03-27 中华人民共和国南京出入境检验检疫局 Method for acquiring digital focused images of fiber cross section slices under microscope
CN104181685A (en) * 2014-07-29 2014-12-03 杭州卓腾信息技术有限公司 Automatic digital slide focusing device and method based on microscope
US20150030242A1 (en) * 2013-07-26 2015-01-29 Rui Shen Method and system for fusing multiple images
CN104516085A (en) * 2013-09-30 2015-04-15 香港纺织及成衣研发中心有限公司 Quick focusing method and device for multispectral imaging
CN104568711A (en) * 2014-12-29 2015-04-29 中国科学院长春光学精密机械与物理研究所 Automatic focusing device for imaging flow cytometer
CN105136292A (en) * 2015-05-06 2015-12-09 中北大学 Aberration compensation method based on AOTF multispectral imaging system

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2610349Y (en) * 2003-04-01 2004-04-07 北京舒尔德科技发展有限公司 Multifunction micro-imaging instrument
CN101706609A (en) * 2009-11-23 2010-05-12 常州达奇信息科技有限公司 Image processing based fast automatic focusing method of microscope
CN101840055A (en) * 2010-05-28 2010-09-22 浙江工业大学 Video auto-focusing system based on embedded media processor
CN102998787A (en) * 2012-07-24 2013-03-27 中华人民共和国南京出入境检验检疫局 Method for acquiring digital focused images of fiber cross section slices under microscope
US20150030242A1 (en) * 2013-07-26 2015-01-29 Rui Shen Method and system for fusing multiple images
CN104516085A (en) * 2013-09-30 2015-04-15 香港纺织及成衣研发中心有限公司 Quick focusing method and device for multispectral imaging
EP2866006A8 (en) * 2013-09-30 2015-06-10 The Hong Kong Research Institute of Textiles and Apparel Limited Fast focusing method and device for multi-spectral imaging
CN104181685A (en) * 2014-07-29 2014-12-03 杭州卓腾信息技术有限公司 Automatic digital slide focusing device and method based on microscope
CN104568711A (en) * 2014-12-29 2015-04-29 中国科学院长春光学精密机械与物理研究所 Automatic focusing device for imaging flow cytometer
CN105136292A (en) * 2015-05-06 2015-12-09 中北大学 Aberration compensation method based on AOTF multispectral imaging system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王魏 等: "多光谱成像系统的快速调焦方法", 《光电工程》 *

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107395993B (en) * 2017-09-08 2023-06-30 北京睿智奥恒视觉科技有限公司 Full-automatic focusing method and system
CN107395993A (en) * 2017-09-08 2017-11-24 北京睿智奥恒视觉科技有限公司 Full-automatic focusing method and system
CN109239900A (en) * 2018-11-07 2019-01-18 华东师范大学 A kind of full-automatic quick focusing method for the big visual field acquisition of microscopic digital image
CN109374621A (en) * 2018-11-07 2019-02-22 杭州迪英加科技有限公司 Focusing method, system and the device of slice scanner
CN109995998A (en) * 2019-01-03 2019-07-09 中国科学院生物物理研究所 A kind of auto focusing method being imaged suitable for scanning/transmission electron microscope
CN109995998B (en) * 2019-01-03 2020-06-12 中国科学院生物物理研究所 Automatic focusing method suitable for scanning/transmission electron microscope imaging
CN110278383A (en) * 2019-07-25 2019-09-24 浙江大华技术股份有限公司 Focus method, device and electronic equipment, storage medium
CN112312120A (en) * 2019-07-30 2021-02-02 深圳光启空间技术有限公司 Automatic adjusting system and method for camera focal plane
CN110488481A (en) * 2019-09-19 2019-11-22 广东工业大学 A kind of microscope focusing method, microscope and relevant device
CN111142253A (en) * 2019-12-05 2020-05-12 苏州大学 Focusing window selection method and device in microscopic image
CN111665617A (en) * 2020-06-24 2020-09-15 武汉中纪生物科技有限公司 Focusing method and system
CN112165570A (en) * 2020-08-29 2021-01-01 南京理工大学 Multi-depth target focusing method based on ghost imaging calculation
CN112230491A (en) * 2020-10-30 2021-01-15 广西代达科技有限公司 Application method of technical camera capable of automatically focusing
CN114449152A (en) * 2020-10-30 2022-05-06 北京小米移动软件有限公司 Focusing method and device, electronic equipment and storage medium
CN114449152B (en) * 2020-10-30 2023-09-22 北京小米移动软件有限公司 Focusing method, focusing device, electronic equipment and storage medium
CN112887604A (en) * 2021-01-26 2021-06-01 深圳赛动生物自动化有限公司 Stem cell image acquisition method, device, system and medium
CN113176274A (en) * 2021-03-19 2021-07-27 哈工大机器人(中山)无人装备与人工智能研究院 Automatic focusing method, device and system for detecting defects of display panel
CN113852761A (en) * 2021-09-27 2021-12-28 宁波华思图科技有限公司 Automatic focusing method of intelligent digital microscope
CN113852761B (en) * 2021-09-27 2023-07-04 宁波华思图科技有限公司 Automatic focusing method for intelligent digital microscope
CN115079372A (en) * 2022-05-05 2022-09-20 深圳安侣医学科技有限公司 Rapid focusing method of microscopic image acquisition device and microscopic image acquisition method
CN115314633A (en) * 2022-06-27 2022-11-08 中国科学院合肥物质科学研究院 Camera focusing method and device computer device and storage medium
CN115314633B (en) * 2022-06-27 2023-06-16 中国科学院合肥物质科学研究院 Camera focusing method, camera focusing device, computer equipment and storage medium

Similar Documents

Publication Publication Date Title
CN106303242A (en) The focusing system quickly of muti-spectrum imaging and method
CN109451244B (en) Automatic focusing method and system based on liquid lens
CN109521547B (en) Variable-step-length automatic focusing method and system
CN107529011B (en) A kind of motorized zoom lens control method
CN103217855B (en) Automatic focusing method of camera
CN105578029B (en) A kind of auto-focusing searching algorithm of multi-scale variable step size
TWI281593B (en) Projector with automatic focus adjustment
CN103973957B (en) Binocular 3D automatic focusing system for camera and method
CN109714519B (en) Method and system for automatically adjusting image frame
CN106210520B (en) A kind of automatic focusing electronic eyepiece and system
CN105378534B (en) The method of work of camera device, camera device
CN108259753A (en) A kind of camera auto-focusing method and device that climbing method is improved based on defocus estimation
WO2021057422A1 (en) Microscope system, smart medical device, automatic focusing method and storage medium
CN105306825A (en) Novel infrared image focusing system and use method thereof
US10534164B2 (en) Digital microscope and focusing method thereof
CN112203012B (en) Image definition calculation method, automatic focusing method and system
CN103020612A (en) Device and method for acquiring iris images
CN1892401A (en) Multi-stage automatic focusing method according to time-frequency domain catched by iris picture
CN105785561B (en) A kind of digital microscope and its focus method
CN109495626A (en) A kind of shooting auxiliary device and system for portable mobile communication equipment
CN106646694A (en) Bionic vision imaging technology based on visible light and near-infrared rays
CN110646933A (en) Automatic focusing system and method based on multi-depth plane microscope
CN106412400A (en) Bionic vision imaging system based on visible light and near infrared and focusing method thereof
CN107806830A (en) A kind of range unit and application method based on zoom camera
CN105025219A (en) Image acquisition method

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20170104