CN106303242A - The focusing system quickly of muti-spectrum imaging and method - Google Patents
The focusing system quickly of muti-spectrum imaging and method Download PDFInfo
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- CN106303242A CN106303242A CN201610688593.4A CN201610688593A CN106303242A CN 106303242 A CN106303242 A CN 106303242A CN 201610688593 A CN201610688593 A CN 201610688593A CN 106303242 A CN106303242 A CN 106303242A
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/60—Control of cameras or camera modules
- H04N23/67—Focus control based on electronic image sensor signals
Abstract
The invention provides a kind of focusing system quickly and the method for muti-spectrum imaging, including: speed image capture module, focusing window choose module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically;Rapid image acquisition module is for the multispectral microscopic image gathered;Focusing window is automatically chosen module and is found out object-image section in multispectral microscopic image as the fine-focused area-of-interest of needs, and the positional information of focusing window is supplied to definition automatic Evaluation module;Definition automatic Evaluation module goes out definition evaluation of estimate according to the positional information calculation of the multispectral microscopic image adopted in real time and focusing window;Before search strategy judge module definition evaluation of estimate and multispectral microscopic image, the definition evaluation of estimate of 5 two field pictures judges focusing position state, and finds focus position by motor control feedback module.The present invention without Human disturbance, accuracy is high, precision high, focusing speed is fast.
Description
Technical field
The present invention relates to micro-image actively focusing field, in particular it relates to a kind of muti-spectrum imaging is quick main
Dynamic focusing system and method.
Background technology
At present, the active focusing method at muti-spectrum imaging mainly has telemetry and image method.Telemetry is mainly
Utilize the range units such as laser range finder, the distance (i.e. object distance) of detection target is measured, public in conjunction with geometric optics newton
Formula, adjusts accordingly object lens height, and then realizes the rapid focus of system.The method is simple and quick, but needs additionally to increase
If auxiliary bodies such as range units, add complexity and the infrastructure cost of system.Additionally, this contactless range finding is often
Owing to external environmental interference factor causes serious range error, cause auto-focusing failure.Image method is mainly by adopting
Collect the shade of gray of image of different out-of-focus appearances, the spectral characteristic etc. obtained and carry out Treatment Analysis, extract and focus accurately
Positional information, realizes the auto-focusing of system by object lens height adjustment.Image method simple in construction, be affected by the external environment little,
And neatly target interested in visual field can be carried out auto-focusing, have become as the main flow side in auto-focusing field
Method.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide the most right of a kind of muti-spectrum imaging
Burnt system and method.
The focusing system quickly of the muti-spectrum imaging according to present invention offer, including: speed image capture module,
Focusing window chooses module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically;Its
In:
Described rapid image acquisition module, for Real-time Collection multispectral microscopic image and multispectral micro-by gather
Image transmitting automatically chooses module and definition automatic Evaluation module to focusing window;
Described focusing window chooses module automatically, for finding out object-image section in multispectral microscopic image as needing
Want fine-focused area-of-interest, and the positional information of focusing window is supplied to definition automatic Evaluation module;
Described definition automatic Evaluation module, for according to the multispectral microscopic image adopted in real time and the position of focusing window
Information calculates definition evaluation of estimate, and described definition evaluation of estimate is transferred to search strategy judge module;
Described search strategy judge module, for according to the definition evaluation of estimate received and described multispectral microscopic image
Above the definition evaluation of estimate of 5 two field pictures judges that focusing position is in out-of-focus appearance or overfocus state, and according to clearly
The unimodality of degree evaluation function determines the direction of motion of object lens, sends Motion to motor control feedback module;
Described motor control feedback module, adjusts with the step-length set for the Motion according to search strategy judge module
Joint, to object lens height and position, finds focus position, completes actively to focus.
According to the focusing method quickly of the muti-spectrum imaging that the present invention provides, comprise the steps:
Rapid image acquisition step: Real-time Collection multispectral microscopic image;
The automatic selecting step of focusing window: find out object-image section in multispectral microscopic image the most poly-as needs
Burnt area-of-interest, and record the positional information of focusing window;
Definition automatic Evaluation step: according to the multispectral microscopic image adopted in real time and the positional information calculation of focusing window
Go out definition evaluation of estimate;
Search strategy judges step: according to 5 two field pictures clear before definition evaluation of estimate and described multispectral microscopic image
Clear degree evaluation of estimate judges focusing position state in which, and determines the fortune of object lens according to the unimodality of sharpness evaluation function
Dynamic direction, generates Motion;
Motor control feedback step: according to Motion with the step-length regulation object lens height and position set, find accurate burnt position
Putting, terminate actively to focus flow process.
Preferably, the automatic selecting step of described focusing window includes: when target relativelys close to field of view center position, it is assumed that
The size of image is M × N, selectedFavored area is treated as focusing window in region, treats the X of favored area, Y side at this respectively
To the maximum finding projection module of differentialsMax | h (y) ' |, the most respectively withMax | h (y) ' | for
CenterRegion is as focusing window.
Preferably, described definition automatic Evaluation step includes: use Gray Projection differential method to carry out computing of focusing, i.e.
Using the Gray Projection first differential 1 norm average of image as sharpness evaluation function, specifically, comprise the steps:
Step A1: calculate image in focusing window respectively at projection h (x) of X and Y-direction and h (y);
Step A2: ask for projecting 1 norm h (x') and the h (y') of the first differential of h (x) and h (y) respectively;
Step A3: calculate h (x') and average P of h (y') respectivelyAvgxAnd PAvgy;
Step A4: calculate PAvgxAnd PAvgyRoot-mean-square PAvg, it is definition evaluation of estimate.
Preferably, described search strategy judges that step includes: utilize ramping constraint to obtain current microcobjective height
The definition evaluation of estimate of the multispectral micro-image of position, it is judged that focusing position is in out-of-focus appearance or overfocus state, if
It is in out-of-focus appearance, then drives driven by motor object lens to move, if being in the direction near target object with the step-length set
Coke-like state, then drive driven by motor object lens to move to the direction of wide object with the step-length set.
Preferably, described motor control feedback step includes:
Step B1: be adjusted object lens height and position according to the step-length set, line definition of going forward side by side evaluation of estimate calculates;
Step B2: judge whether the definition evaluation of estimate obtained reaches definition appraisal curve peak value, if then terminating stream
Journey;Step B1 is performed if it is not, then return;
Wherein: microscope is adjusted to definition appraisal curve peak and is focus position.
Compared with prior art, the present invention has a following beneficial effect:
1, the present invention provides the focusing system quickly of muti-spectrum imaging and method focusing speed are fast, precision
Height, good stability, and simple in construction, autgmentability is strong.
2, the present invention provides the focusing system quickly of muti-spectrum imaging and method are without manual intervention, it is possible to
Independently complete focusing task in real time.
Accompanying drawing explanation
By the detailed description non-limiting example made with reference to the following drawings of reading, the further feature of the present invention,
Purpose and advantage will become more apparent upon:
The theory diagram of the focusing system quickly of the muti-spectrum imaging that Fig. 1 provides for the present invention;
The hardware structure diagram of the focusing system quickly of the muti-spectrum imaging that Fig. 2 provides for the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area
Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area
For personnel, without departing from the inventive concept of the premise, it is also possible to make some changes and improvements.These broadly fall into the present invention
Protection domain.
The focusing system quickly of the muti-spectrum imaging according to present invention offer, including: rapid image gathers mould
Block, focusing window choose module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically
Five part compositions.
Rapid image acquisition module, for Real-time Collection multispectral microscopic image, and the multispectral microscopic image that will gather
It is transferred to focusing window and automatically chooses module and definition automatic Evaluation module;
Focusing window chooses module automatically, participates in what sharpness evaluation function calculated for automatically finding out in picture in its entirety
Image window;The Rational choice of focusing window is a key factor of focusing precision.Focusing window is chosen the biggest, and operand is more
Greatly, but also may result in target background and participate in computing, affect the focusing accuracy of target.Focusing window is too small, may
Lose the area-of-interest partial information of target, affect focus effects.
When target relativelys close to field of view center position, it is assumed that the size of image is M × N, selectedRegion conduct
Focusing window treat favored area, respectively at the X in this region, Y-direction find projection module of differentials maximum max | h (x) ' |, max |
H (y) ' |, the most respectively with max centered by | h (x) ' |, max | h (y) ' |Region is as focusing window.
Additionally, generally include two parts of target and background in image, target part is that needs are fine-focused interested
Region, background is then the part non-interested allowing non-accurate focusing, therefore, also should note during focusing window is chosen
The selection of meaning the window's position.
Definition automatic Evaluation module, for measuring the readability of image, reflects that be exactly to image in image procossing
Acutance or perhaps a kind of sign of gradient.
During auto-focusing, image deviation focus position is the most remote, and image is the most unintelligible, the edge ladder that integral inverted mirrors
Degree information is the fewest;Otherwise, closer to focus position, image is the most clear, and image integral edge gradient information is the abundantest;In accurate burnt position
Putting place, image is the most clear, and the edge gradient information of image is the abundantest.The metric function that definition is evaluated is during auto-focusing
It is equivalent to a kind of algorithm " detector ", comes according to its calculated definition evaluation of estimate in the search procedure of focus position
Carry out the adjustment of optical texture.Therefore, the quality of sharpness evaluation function performance is the key factor determining auto-focusing effect.
Search strategy judge module, for referring to search for the side of accurate focusing position according to calculated definition evaluation of estimate
Method, search strategy then determines the time of whole auto-focusing process.
Motor control feedback module, for according to set search strategy, according to calculated definition evaluation of estimate pair
Object lens height and position carries out micro-positioning regulators, position after adjustment, and the definition evaluation of estimate re-starting image calculates, then same
Sample, according to search strategy before, adjusts object lens height and position, again and so forth, until imaging is adjusted to definition and evaluates song
Line peak, this position is focus position, and whole active focus process terminates.
Specifically, as in figure 2 it is shown, the focusing system quickly of muti-spectrum imaging includes: CCD camera, micro-thing
Mirror, PC, MCU control module, light source, focusing drive mechanism, wherein MCU control module comprises microcontroller, motor drives
Dynamic device, focusing drive mechanism comprises motor, ball-screw.First pass through rapid image acquisition module, through microscope imaging
To CCD camera, the image collected is transferred to PC by USB interface;PC utilizes focusing window automatically to choose module with clear
Clear degree automatic Evaluation module, passes judgment on the definition of the image collected, and according to search strategy judge module, calculates
The step-length of focusing next step motion of motor and direction, utilize motor control feedback module, sends control instruction to micro-by serial ports
Controller, microprocessor controls driving system of stepping motor is overall to be regulated towards imaging direction the most clearly, forms a closed loop
The loop of regulation, continuous repetitive cycling, until the image collected meets the requirements.Simultaneously take account of the need of field luminance
, PC can send instruction, the brightness of regulation light source to controller.
The feature that operand that described definition automatic Evaluation module make use of Gray Projection is little, real-time, by gray scale
Projection thought is incorporated in auto-focusing algorithm, uses focusing degree of depth Atomatic focusing method based on projection differentiation.The method
It is as sharpness evaluation function using the Gray Projection first differential 1 norm average of image, to realize this multi-optical spectrum imaging system
Fast automatic focusing.
Further, the focusing method quickly of the muti-spectrum imaging in the present invention, comprise the steps:
Step S1: the determination of focusing window: before carrying out auto-focusing, first according to observation needs, in conjunction with present frame figure
As carrying out the calculating of auto-focusing window position.
Step S2: target image definition values calculates:
Step S2.1: calculate image in focusing window respectively at projection h (x) of X and Y-direction and h (y);
Step S2.2: ask for 1 norm h (x') and the h (y') of its first differential respectively;
Step S2.3: calculate h (x') and average P of h (y') respectivelyAvgxAnd PAvgy;
Step S2.4: calculate PAvgxAnd PAvgyRoot-mean-square PAvg, it is the definition evaluation of estimate of this frame.
Step S3: utilize ramping constraint, at the calculated current microcobjective height and position of step S2
Micro-image definition evaluation of estimate, is sequentially adjusted in object lens height and position, repeats step S2, until searching out microcobjective height
Focus position.
Step S4: actively focus process terminates.
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make a variety of changes within the scope of the claims or revise, this not shadow
Ring the flesh and blood of the present invention.In the case of not conflicting, the feature in embodiments herein and embodiment can any phase
Combination mutually.
Claims (6)
1. the focusing system quickly of a muti-spectrum imaging, it is characterised in that including: speed image capture module, right
Burnt window chooses module, definition automatic Evaluation module, search strategy judge module, motor control feedback module automatically;Wherein:
Described rapid image acquisition module, for Real-time Collection multispectral microscopic image, and the multispectral microscopic image that will gather
It is transferred to focusing window and automatically chooses module and definition automatic Evaluation module;
Described focusing window chooses module automatically, for finding out object-image section in multispectral microscopic image as needs standard
The area-of-interest really focused on, and the positional information of focusing window is supplied to definition automatic Evaluation module;
Described definition automatic Evaluation module, for according to the multispectral microscopic image adopted in real time and the positional information of focusing window
Calculate definition evaluation of estimate, and described definition evaluation of estimate is transferred to search strategy judge module;
Described search strategy judge module, before according to the definition evaluation of estimate received and described multispectral microscopic image
The definition evaluation of estimate of 5 two field pictures judges that focusing position is in out-of-focus appearance or overfocus state, and comments according to definition
The unimodality of valency function determines the direction of motion of object lens, sends Motion to motor control feedback module;
Described motor control feedback module, right with the step-length regulation set for the Motion according to search strategy judge module
Object lens height and position, finds focus position, completes actively to focus.
2. the focusing method quickly of a muti-spectrum imaging, it is characterised in that comprise the steps:
Rapid image acquisition step: Real-time Collection multispectral microscopic image;
The automatic selecting step of focusing window: find out object-image section in multispectral microscopic image fine-focused as needs
Area-of-interest, and record the positional information of focusing window;
Definition automatic Evaluation step: go out clearly according to the positional information calculation of the multispectral microscopic image adopted in real time and focusing window
Clear degree evaluation of estimate;
Search strategy judges step: according to the definition of 5 two field pictures before definition evaluation of estimate and described multispectral microscopic image
Evaluation of estimate judges focusing position state in which, and determines the motion side of object lens according to the unimodality of sharpness evaluation function
To, generate Motion;
Motor control feedback step: according to Motion with the step-length regulation object lens height and position set, find focus position, knot
Restraint flow process of actively focusing.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described focusing
The automatic selecting step of window includes: when target relativelys close to field of view center position, it is assumed that the size of image is M × N, selectedFavored area is treated as focusing window in region, treats the X of favored area at this respectively, and Y-direction finds projection module of differentials
| h (x) ' |, max | the h (y) ' | that is worth greatly max, the most respectively with max centered by | h (x) ' |, max | h (y) ' |Region is made
For focusing window.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described clearly
Degree automatic Evaluation step includes: use Gray Projection differential method to carry out computing of focusing, will the Gray Projection single order of image micro-
Divide 1 norm average as sharpness evaluation function, specifically, comprise the steps:
Step A1: calculate image in focusing window respectively at projection h (x) of X and Y-direction and h (y);
Step A2: ask for projecting 1 norm h (x') and the h (y') of the first differential of h (x) and h (y) respectively;
Step A3: calculate h (x') and average P of h (y') respectivelyAvgxAnd PAvgy;
Step A4: calculate PAvgxAnd PAvgyRoot-mean-square PAvg, it is definition evaluation of estimate.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described search
Strategy judges that step includes: utilize ramping constraint to obtain the clear of multispectral micro-image at current microcobjective height and position
Clear degree evaluation of estimate, it is judged that focusing position is in out-of-focus appearance or overfocus state, if the out-of-focus appearance of being in, then drives motor belt motor
Animal mirror moves to the direction near target object with the step-length set, if being in overfocus state, then drives driven by motor object lens
Move to the direction of wide object with the step-length set.
The focusing method quickly of muti-spectrum imaging the most according to claim 2, it is characterised in that described motion
Control feedback step to include:
Step B1: be adjusted object lens height and position according to the step-length set, line definition of going forward side by side evaluation of estimate calculates;
Step B2: judge whether the definition evaluation of estimate obtained reaches definition appraisal curve peak value, if then terminating flow process;If
No, then return and perform step B1;
Wherein: microscope is adjusted to definition appraisal curve peak and is focus position.
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CN112230491A (en) * | 2020-10-30 | 2021-01-15 | 广西代达科技有限公司 | Application method of technical camera capable of automatically focusing |
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CN114449152B (en) * | 2020-10-30 | 2023-09-22 | 北京小米移动软件有限公司 | Focusing method, focusing device, electronic equipment and storage medium |
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