CN106290771B - The detection device of nano material-pollution objects system biological effect and application - Google Patents
The detection device of nano material-pollution objects system biological effect and application Download PDFInfo
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- CN106290771B CN106290771B CN201610872500.3A CN201610872500A CN106290771B CN 106290771 B CN106290771 B CN 106290771B CN 201610872500 A CN201610872500 A CN 201610872500A CN 106290771 B CN106290771 B CN 106290771B
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Abstract
The invention discloses a kind of detection device of nano material pollution objects system biological effect, for small-sized aquatic nekton, including biological culture system and biological shielding system on its interior；Biological culture system includes cylinder and outer cover at its upper end；Biological shielding system includes built-in cylindrical hole, and built-in cylindrical hole is located in cylinder, and the built-in cylindrical hole of non-woven fabrics interlayer is equipped with including at least three common built-in cylindrical holes and a middle part.The invention also discloses a kind of applications of the detection device of nano material pollution objects system biological effect.The advantage of the invention is that：Nano material aggregation, sedimentation can be reflected and change with pollutant different water levels concentration caused after the mutual adsorption process of pollutant by providing one kind, place that simple, temperature parameter is controllable, at the same reflect nano material returning to environmental pollutants become process and cover different water levels control setting nano material pollutant complex system biological effect detection device and application.
Description
Technical field
The present invention relates to ecological risk assessment field more particularly to a kind of nano material-pollution objects system biological effects
Detection device and application.
Background technology
Nanometer technology is known as being one of three big mainstay of 21 century.Since its special nature nano material extensively should
For every field, but nano material will inevitably enter environment and the ecosystem during using and discarding, from
And cause various biological effect.At present, the biological effect research of nano material is limited to single substance in laboratory conditions
Toxic effect, however in practical natural environment, nano material is often existed simultaneously with multiple pollutant and is occurred complicated
Interaction.Nano material has the specific surface area of bigger relative to macroscopic particles object, once into environment, can significantly affect
With change the environmental behaviour of pollutant in environment or lead to the change of pollutant biological effectiveness and poisonous effect.Such as nano-silica
Change titanium because its specific surface area height and unique surface chemical property frequently as adsorbent come handle heavy metal in water environment and
Organic pollution, while water body is also considered as the final place that nano-titanium dioxide recycles accumulation in the environment, therefore it can
It is come into full contact with various pollutants, and it is possible that adsorb a large amount of pollutants increases it in aquatile body as the carrier of poisonous substance
Total amount, and then amplify toxic effect.At present, still locate about the research to interact between nano material carrier and environmental contaminants
Starting stage, joint toxicity are urgently furtherd investigate.
How to simulate and react the interaction in true environment doped with the binary of nano material and polynary pollutant, ring
Border process and toxicity variation are nano material Environment release and the emphasis and difficult point of environmental health assessment.However it grinds in this regard
Us are studied carefully while increasing year by year it is not difficult to find that the existing big multimode of research is single, are exposed to " nanometer material frequently with biological subject
The simple mode of biological effect compared with the control group after material-pollutant " mixed liquor is up to final conclusion, but nano material itself
With various features, there is settlement action simultaneously can effectively adsorb multiple pollutant mostly, aggregation settlement action is causing to pollute
It while object concentration locally increases, can also obtain opposite using the experimental animal for moving in different water levels as a result, different water are special
The nano material of property also results in the different environment of complex and returns.But existing correlative study carries out in a singular fashion,
It most experiment condition and is differed in research, obtained conclusion also emphasizes particularly on different fields, and does not consider the pollutant ring in true water sample
Border process returns to become with distribution, often using a kind of direct result of animal subject as nano material-pollutant complex system environment
The final conclusion of effect causes existing result of study not fully consistent, therefore is ground in relevant research mode with without exception complete
The method of studying carefully needs further to be improved and optimization.
Therefore, it makes a kind of available for distinguishing the simple of nano material-pollutant complex system different component biological effect
Device has the easily-controllable conditions of each parameter such as the simple, temperature of placement, at the same can reflect nano material to environmental pollutants return
It acts on and covers different water levels control setting, there is very important application value, while be also a technological difficulties.The present invention
Exactly in view of the above-mentioned problems, emphasis different component pollutant biological effect and water layer in reaction true environment return and become etc.
Carry out certain improvement.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of suitable for small-sized aquatic nekton
Caused pollutant different water levels concentration changes after can reflecting nano material and a series of environmental processes of pollutant, place it is simple,
Temperature parameter is controllable, while can reflect the nano material-pollution objects system life for returning the process that becomes of nano material to environmental pollutants
The detection device of object effect and application.
The present invention is achieved by the following technical solutions, and the invention discloses a kind of nano material-pollution objects system lifes
The detection device of object effect, for small-sized aquatic nekton, including biological culture system and biology isolation on its interior
System；The biological culture system includes cylinder and outer cover at its upper end；The biology shielding system includes built-in cylindricality
Hole, the built-in cylindrical hole are located in cylinder, further include at least three common built-in cylindrical holes and are set with a middle part
There is the built-in cylindrical hole of non-woven fabrics interlayer.
As one of preferred embodiment of the present invention, the cylinder is the cylinder of opening upwards, the outer cover for opening to
Under round outer cover；The opening diameter of the cylinder is slightly less than the opening diameter of outer cover, and the height of the outer cover is less than cylinder
Highly.
As one of preferred embodiment of the present invention, the built-in cylindrical hole is vertically installed in the bottom surface of cylinder, and with cylinder
The bottom surface of body is seamlessly connected；The height of the built-in cylindrical hole is slightly less than the height of cylinder.
As one of preferred embodiment of the present invention, the cylinder is the cylinder equipped with double casing, under the cylinder both sides
A trepanning is further respectively had on the outermost layer housing at end to connect outside.
One of preferred embodiment as the present invention, further includes temperature control system, and the temperature control system includes disengaging
Water system, constant temperature water bath controller, temperature detector, temperature measuring probe；
The inlet and outlet system include positioned at cylinder double casing between around wall water pipe and its both ends water inlet, be discharged
Mouthful, the water inlet, water outlet are respectively arranged at two tappings on the outermost layer housing of cylinder both sides lower end, are all connected to
Constant temperature water bath controller；
The temperature measuring probe is three, and the top between cylinder double casing, middle part and bottom, are all connected with respectively
In temperature detector.
One of preferred embodiment as the present invention, the constant temperature water bath controller is interior to be equipped with suction pump.
One of preferred embodiment as the present invention, the temperature detector is interior to be equipped with the warm alarm of limit.
The invention also discloses the application of the detection device of above-mentioned nano material-pollution objects system biological effect, including with
Lower step：
(1) the division of control group and experimental group：
The built-in cylindrical hole of the detection device of above-mentioned nano material-pollution objects system biological effect is grouped, is set
One control group and four experimental groups, respectively：Blank control group, single nano material exposure group, without nano material doping
Pollutant exposure group, the same concentration pollutant exposure group of the first extra implant nano material, the second extra implant nano material
With concentration pollutant exposure group, wherein, the same concentration pollutant exposure group of the first extra implant nano material is additionally mixed with second
It is separated between the same concentration pollutant exposure group of miscellaneous nano material by non-woven fabrics；In addition, experimental group can be separately set as needed；
(2) the preparation of pollutant exposure liquid：
Liquid is exposed for each group pollutant by the group system of step (1) respectively, wherein the nanometer of single nano material exposure group
The same concentration pollutant exposure group of material concentration and the first extra implant nano material, the second extra implant nano material it is same dense
The nano material concentration for spending pollutant exposure group is identical, the pollutant concentration of the pollutant exposure group of no nano material doping and the
The same concentration pollutant exposure of the same concentration pollutant exposure group, the second extra implant nano material of one extra implant nano material
The pollutant concentration of group is identical；After the certain environment time, the same concentration of combined exposure group, i.e. the first extra implant nano material is dirty
The complex system for contaminating the same concentration pollutant exposure group of object exposure group the+the second extra implant nano material reaches stable state；
(3) sampling and materialization phenetic analysis：
After nano material and pollutant are incubated a period of time, the same concentration of the first extra implant nano material is first taken to pollute
Object exposure group, then the solution of the same concentration pollutant exposure group of the second extra implant nano material is taken to carry out nano material and pollution
The materialization phenetic analysis that materialization interacts between object；
(4) the processing of experimental animal：
Growth period synchronization process is carried out to experimental animal, is then used for growth period consistent experimental animal follow-up real
It tests；
(5) the addition of experimental animal and the variation of biological effect：
Experimental animal and its food equivalent are put into each group, constant temperature incubation for a period of time after, detach biological sample, carry out dirty
Contaminate the detection of object biological effectiveness.
One of preferred embodiment as the present invention, the detection method of materialization phenetic analysis is inductance coupling in the step (3)
Close plasma atomic emission spectroscopy, x-ray photoelectron spectroscopy, power spectrum and infrared spectrum.
One of preferred embodiment as the present invention, pollutant biological effectiveness detection method is to each in the step (5)
Group experimental animal carries out respective embryo toxicity test and growth form is recorded.
The present invention compared with prior art the advantages of be：Device provided by the invention is small suitable for Daphnia magna, zebra fish etc.
The aquatic nekton of type, be integrated with shape it is small it is easily-controllable, comparison clearly, temperature-controllable, can be to nano material-pollutant complex system
Environmental process layering, the function of packet decomposition analysis.In the present apparatus non-woven fabrics design the water layer of its aquatile can be distributed into
Row defines, but to the environmental process of nano material and pollutant without iris action, while complex system stability is not destroyed,
The water layer toxicity variation of complex system after certain environment process is reflected using the experimental animal of layering life.Apparatus of the present invention
Using a platform can be provided for the various sign researchs of animal subject so that common pattern biology is utilized to carry out quickly biology
It monitors to evaluate the ecological risk and health effectiveness that the binary pollutant of doped nano-material in true environment interacts
It is possibly realized.The device uses simple, the popularization and application of suitable correlative study.
Description of the drawings
Fig. 1 is the part-structure signal of the detection device of nano material-pollution objects system biological effect in embodiment 1
Figure；
Fig. 2 is the overall structure signal of the detection device of nano material-pollution objects system biological effect in embodiment 1
Figure；
Fig. 3 is the structural representation of the application of the detection device of nano material-pollution objects system biological effect in embodiment 1
Figure.
In figure：1 is cylinder, and 2 be outer cover, and 3 be double casing, and 4 be built-in cylindrical hole, and 41 be common built-in cylindrical hole, 42
The built-in cylindrical hole of non-woven fabrics interlayer is equipped with for middle part, 5 be non-woven fabrics, and 6 be constant temperature water bath controller, and 7 be temperature detector, 8
For temperature measuring probe, 9 be around wall water pipe, and 10 be water inlet, and 11 be water outlet, and 12 be zebra fish, and 13 be blank control group, 14
For single nano material exposure group, the 15 pollutant exposure group for the doping of no nano material, 16 be the first extra implant nanometer material
The same concentration pollutant exposure group of material, 17 be the same concentration pollutant exposure group of the second extra implant nano material.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment is carried out lower based on the technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
As Figure 1-Figure 2, the detection device of a kind of nano material of the present embodiment-pollution objects system biological effect is fitted
For small-sized aquatic nekton, including biological culture system, positioned at the biological shielding system and temperature of biological culture internal system
Spend control system.
Biological culture system include cylinder 1 and outer cover 2 at its upper end, cylinder 1 for the opening equipped with double casing 3 to
On cylinder, further respectively have two trepannings on the outermost layer housing of 1 both sides lower end of cylinder to connect outside, outer cover 2 is opens
The downward round outer cover of mouth, wherein, the opening diameter of cylinder 1 is slightly less than the opening diameter of outer cover 2, and the height of outer cover 2 is less than cylinder
The height of body 1.
Biological shielding system includes being vertically installed in 1 bottom surface of cylinder and the built-in cylindrical hole 4 being seamlessly connected therewith, built-in column
Shape hole 4 is located in cylinder 1, and height is slightly less than the height of cylinder 1, and built-in cylindrical hole 4 is further common interior including three
It puts cylindrical hole 41 and a middle part is equipped with the built-in cylindrical hole 42 of non-woven fabrics interlayer.The design of non-woven fabrics 5 can be to experimental animal
Water layer distribution is bound, but can not destroy complex system without iris action to the environmental process of nano material and pollutant
While stability, the water layer toxicity change of complex system after certain environment process is reflected using the experimental animal of layering life
Change.
Temperature control system includes inlet and outlet system, constant temperature water bath controller 6, temperature detector 7, temperature measuring probe 8；
Inlet and outlet system includes the water inlet 10 around wall water pipe 9 and its both ends, the water outlet 11 between 1 double casing 3 of cylinder, into
The mouth of a river 10, water outlet 11 are respectively arranged at two tappings on the outermost layer housing of 1 both sides lower end of cylinder, are all connected to inside
Constant temperature water bath controller 6 equipped with suction pump.When experimental animal is cultivated in built-in cylindrical hole 4, water-bath is set in advance
The bath temperature of thermostatic controller 6, then by suction pump by thermostatted water by water inlet 10 by being uniformly surrounded on cylinder around wall water pipe 9
It between the double casing 3 of body 1, and is discharged by water outlet 11, is finally recycled to constant temperature water bath controller 6, ensure whole device temperature
The homogeneity of degree.
Temperature measuring probe 8 is three, and top, middle part and the bottom between 1 double casing 3 of cylinder, are all connected with respectively
In the temperature detector 8 for being internally provided with the warm alarm of limit.When experimental animal is cultivated in built-in cylindrical hole 4, set in advance
The alarm temperature of the warm alarm of limit is put, when the unit temp that temperature measuring probe 8 detects then sends out alarm more than alarm temperature.
As Figure 2-3, a kind of detection using above-mentioned nano material-pollution objects system biological effect of the present embodiment fills
The application put, using a kind of sensitive instruction biology-zebra fish 12 of Ecology Environment Evaluation as experimental animal, including following step
Suddenly：
(1) the division of control group and experimental group：
The built-in cylindrical hole 4 of the detection device of above-mentioned nano material-pollution objects system biological effect is grouped, is set
One control group and four experimental groups, respectively：Blank control group 13, single nano material exposure group 14 are mixed without nano material
Miscellaneous pollutant exposure group 15, the same concentration pollutant exposure group 16 of the first extra implant nano material, the second extra implant are received
The same concentration pollutant exposure group 17 of rice material, wherein, the same concentration pollutant exposure group 16 of the first extra implant nano material
It is separated between the same concentration pollutant exposure group 17 of the second extra implant nano material by non-woven fabrics 5；
(2) the preparation of pollutant exposure liquid：
Addition etc. respectively in the built-in cylindrical hole 4 of the detection device of above-mentioned nano material-pollution objects system biological effect
The aeration tap water of volume, then nano-titanium dioxide and heavy metal cadmium are added in each hole by the grouping of step (1) respectively, it prepares
Each group pollutant exposes liquid, specially：(nano-titanium dioxide exposes for blank control group 13, single nano material exposure group 14
Group), without nano material doping pollutant exposure group 15 (heavy metal cadmium exposure group), the first extra implant nano material it is same dense
Spend pollutant exposure group 16 (the heavy metal cadmium exposure group of addition nano-titanium dioxide), the second extra implant nano material it is same dense
Spending pollutant exposure group 17, (there are non-woven fabrics 5 between the same concentration pollutant exposure group 16 of the first extra implant nano material
Addition nano-titanium dioxide heavy metal cadmium exposure group), wherein, the nano-titanium dioxide of single nano material exposure group 14 is dense
Degree and same concentration pollutant exposure group 16, the same concentration of the second extra implant nano material of the first extra implant nano material are dirty
The nano-titanium dioxide concentration for contaminating object exposure group 17 is identical, and the heavy metal cadmium of the pollutant exposure group 15 of no nano material doping is dirty
Contaminate the same concentration pollutant exposure group 16 of concentration and the first extra implant nano material of object, the second extra implant nano material
It is identical with the concentration of the heavy metal cadmium object of concentration pollutant exposure group 17.After the certain environment time, combined exposure group (first
The same concentration pollutant exposure of same concentration pollutant exposure group 16+ the second extra implant nano materials of extra implant nano material
The complex system of group 17) reaches stable state；
(3) sampling and materialization phenetic analysis：
After for 24 hours, each exposure group liquid, the pollutant exposure group 15 adulterated to no nano material, the first extra implant are drawn
The same concentration pollutant exposure group 16 of nano material, the same concentration pollutant exposure group 17 of the second extra implant nano material carry out
Inductively coupled plasma atomic emission spectrum (ICP-AES) detection cadmium concentration variation；First extra implant nano material it is same
The same concentration pollutant exposure group 17 of concentration pollutant exposure group 16 and the second extra implant nano material is by being dried
Afterwards, it interacts to materializations such as its row X-ray photoelectron spectroscopic analysis (XPS), power spectrum (EDX) and infrared spectrums (FT-IR)
Analysis；
(4) the processing of experimental animal：
Zebra fish 12 is placed at laboratory recirculation system to raise using aeration tap water, water body pH is 7, and hardness is
7, maintaining dissolved oxygen using aerator, daily feeding zebra fish is primary, clears up cylinder bottom excrement and remnants in time in saturation state
Skilful material.Experiment is tamed one month or so with adult fish, ensures natural mortality rate≤5% in 7 days.Illumination has certain regularity, light
According to/dark=14h/10h, the interference of the extraneous factors such as artificial, noise is avoided as possible；
In experiment the previous day, selection cultivates steadily in the long term and reaches sexually matured zebra fish 12 by sex ration 1:2 ratio
It is positioned in hatching box, female milter is separated with transparent baffle, and an evening is placed under dark condition.The next morning (about 08:00)
Illumination is given, and takes out baffle, female milter starts mate and oviposit under light stimulation, starts to complete after about 30min after laying eggs
Fertilization.Fertilized eggs are collected, and are cleaned 2 times with standard dilution water, removal of residue is removed, selects the full fertilization of normal fertilization, shape
Ovum is tested for later stage embryotoxicity；
(5) the addition of experimental animal and the variation of biological effect：
12 embryotoxicity of zebra fish tests reference《OECD fish embryo acute toxic test guides (2013)》It carries out, each
Group is separately added into 30 pieces of fertilized eggs and corresponding nutriment, and regulating device temperature is 25 degrees Celsius, the zebra of record after fertilization 96h
The death of 12 embryo of fish and hatching number, while record embryo and hatch the lopsided type and number of juvenile fish；Then, from every group with
Machine selects 15 juvenile fish simultaneously to be anaesthetized with 0.01%MS-222, measures young fish length, that is, juvenile fish from head front end to tail end
Length.
Interpretation of result：
Nano-titanium dioxide can play aggtegation to the heavy metal cadmium in solution, and x-ray photoelectron spectroscopy is to the two
Further verification has been done in interaction.The same concentration pollutant exposure group 16 and second of first extra implant nano material is additional
The cadmium on nano-titanium dioxide surface is adsorbed in nanometer titanium dioxide in the same concentration pollutant exposure group 17 of doped nano-material
The aggregation settlement action of titanium and the same concentration pollutant exposure group 17 for being enriched in the second extra implant nano material, cause mixture
The water layer of cadmium contaminants is unevenly distributed in system.Pollutant exposure group 15, the first extra implant nanometer adulterated to no nano material
Cadmium concentration in the same concentration pollutant exposure group 16 of material, the same concentration pollutant exposure group 17 of the second extra implant nano material
Detection find due to nano-titanium dioxide adsorpting aggregation act on, free cadmium ion quantity decline, eventually lead to the first volume
The same concentration pollutant exposure group 16 of outer doped nano-material survey cadmium concentration decline, the second extra implant nano material it is same dense
Degree pollutant exposure group 17 surveys cadmium concentration rising, this result provides further verification to above-mentioned saying.
In addition research is it has also been found that the same concentration pollutant exposure group 16 and second of the first extra implant nano material is additionally mixed
The cadmium concentration water layer of the same concentration pollutant exposure group 17 of miscellaneous nano material, which is unevenly distributed, leads to incubating for the zebra fish in two groups 12
Rate and body length change.
The introducing of non-woven fabrics 5 exposes the subregion of aquatic travelling biology, makes using a kind of biosimulation and reacts true ring
The interaction of complex system, the pollutant biological effect of different water levels component and environment return to become to being possibly realized in border.
It can be seen that：Device of the present invention and application can be with effectively evaluating nano materials and the phase of environmental contaminants
Interaction can simulate and reflect that pollutant changes in the concentration of different water levels under nano material existence condition in water environment, and
Reflect that different water levels biology returns the effect that becomes to show different responses pollutant with cleverly design.The present invention can also be right
Effective identification is made in the biological effectiveness of pollutant and biological accumulation after interaction.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of detection device of nano material-pollution objects system biological effect, for small-sized aquatic nekton, feature exists
In including biological culture system and biological shielding system on its interior；The biological culture system includes cylinder and is located at
The outer cover of the upper end；The biology shielding system includes built-in cylindrical hole, and the built-in cylindrical hole is located in cylinder, further
Ground includes at least three common built-in cylindrical holes and a middle part is equipped with the built-in cylindrical hole of non-woven fabrics interlayer.
2. the detection device of nano material according to claim 1-pollution objects system biological effect, which is characterized in that institute
The cylinder that cylinder is opening upwards is stated, the outer cover is the round outer cover that Open Side Down；The opening diameter of the cylinder is smaller
In the opening diameter of outer cover, the height of the outer cover is less than the height of cylinder.
3. the detection device of nano material according to claim 1-pollution objects system biological effect, which is characterized in that institute
The bottom surface that built-in cylindrical hole is vertically installed in cylinder is stated, and is seamlessly connected with the bottom surface of cylinder；The height of the built-in cylindrical hole
Degree is slightly less than the height of cylinder.
4. the detection device of nano material according to claim 1-pollution objects system biological effect, which is characterized in that institute
Cylinder is stated as the cylinder equipped with double casing, further respectively had on the outermost layer housing of the cylinder both sides lower end trepanning with
Connection is external.
5. the detection device of nano material according to claim 4-pollution objects system biological effect, which is characterized in that also
Including temperature control system, the temperature control system includes inlet and outlet system, constant temperature water bath controller, temperature detector, temperature
Spend detection probe；
The inlet and outlet system includes the water inlet around wall water pipe and its both ends, the water outlet between cylinder double casing,
The water inlet, water outlet are respectively arranged at two tappings on the outermost layer housing of cylinder both sides lower end, are all connected to water
Bathe thermostatic controller；
The temperature measuring probe is three, and the top between cylinder double casing, middle part and bottom, are all connected to temperature respectively
Spend detector.
6. the detection device of nano material according to claim 5-pollution objects system biological effect, which is characterized in that institute
It states and suction pump is equipped in constant temperature water bath controller.
7. the detection device of nano material according to claim 5-pollution objects system biological effect, which is characterized in that institute
It states and the warm alarm of limit is equipped in temperature detector.
8. a kind of such as detection device of claim 1-7 any one of them nano material-pollution objects system biological effect is answered
With, which is characterized in that include the following steps：
(1) the division of control group and experimental group：
The built-in cylindrical hole of the detection device of above-mentioned nano material-pollution objects system biological effect is grouped, sets one
Control group and four experimental groups, respectively：Blank control group, single nano material exposure group, the pollution without nano material doping
Object exposure group, the same concentration pollutant exposure group of the first extra implant nano material, the second extra implant nano material it is same dense
Pollutant exposure group is spent, wherein, same concentration pollutant exposure group and the second extra implant of the first extra implant nano material are received
It is separated between the same concentration pollutant exposure group of rice material by non-woven fabrics；In addition, experimental group can be separately set as needed；
(2) the preparation of pollutant exposure liquid：
Liquid is exposed for each group pollutant by the group system of step (1) respectively, wherein the nano material of single nano material exposure group
Same concentration pollutant exposure group, the same concentration of the second extra implant nano material of concentration and the first extra implant nano material are dirty
The nano material concentration for contaminating object exposure group is identical, the pollutant concentration and the first volume of the pollutant exposure group of no nano material doping
The same concentration pollutant exposure group of outer doped nano-material, the same concentration pollutant exposure group of the second extra implant nano material
Pollutant concentration is identical；After the certain environment time, the same concentration pollutant of combined exposure group, i.e. the first extra implant nano material
The same concentration pollutant exposure group of exposure group the+the second extra implant nano material, complex system reach stable state；
(3) sampling and materialization phenetic analysis：
After nano material and pollutant are incubated a period of time, the same concentration pollutant for first taking the first extra implant nano material is sudden and violent
Dew group, then the solution of the same concentration pollutant exposure group of the second extra implant nano material is taken to carry out between nano material and pollutant
The materialization phenetic analysis of materialization interaction；
(4) the processing of experimental animal：
Growth period synchronization process is carried out to experimental animal, growth period consistent experimental animal is then used for subsequent experimental；
(5) the addition of experimental animal and the variation of biological effect：
Experimental animal equivalent is put into each group, constant temperature incubation for a period of time after, detach biological sample, carry out pollutant biologically effective
The detection of property.
9. the application of the detection device of nano material according to claim 8-pollution objects system biological effect, feature exist
In the detection method of materialization phenetic analysis is inductively coupled plasma atomic emission spectrum, X ray light in the step (3)
Electron spectrum, power spectrum and infrared spectrum.
10. the application of the detection device of nano material according to claim 8-pollution objects system biological effect, feature
It is, pollutant biological effectiveness detection method is that respective embryo toxicity reality is carried out to each group experimental animal in the step (5)
It tests and growth form is recorded.
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