CN105842411A - System for testing influence of chemical environment of water body on plankton ethology - Google Patents
System for testing influence of chemical environment of water body on plankton ethology Download PDFInfo
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- CN105842411A CN105842411A CN201610171173.9A CN201610171173A CN105842411A CN 105842411 A CN105842411 A CN 105842411A CN 201610171173 A CN201610171173 A CN 201610171173A CN 105842411 A CN105842411 A CN 105842411A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
Abstract
The invention discloses an experimental system for testing the influence of a chemical environment of a water body on plankton ethology. The experimental system comprises a test-bed assembly, an illumination adjusting assembly, a temperature adjusting assembly, a central control assembly and an uninterrupted power source assembly. The test-bed assembly contains multiple liquid storage bags, multiple flow control devices, multiple infusion tubes and a test container. The test container contains a main body device, a central water inlet region, an anti-runaway protection net, a selective test region and multiple glass separator plates. The experimental method is carried out by the experimental system for testing the influence of a chemical environment of a water body on plankton ethology. The experimental system and the experimental method can guarantee accuracy of experimental conditions and accuracy of experiment effect, and are suitable for rapidly verifying behavior changes of zooplankton responding to different concentration levels of different environmental factors or the same environmental factor.
Description
Technical field
The application relates to plankton Behaviors survey research field, and more specifically,
Relate to a kind of testing experimental system and the reality that plankton behavioristics is affected by water body chemical environment
Proved recipe method.
Background technology
Avoidance behaviour is extremely common in nature, is the biological one escaping potential threat
More effective manner (Loose&Dawidowicz 1994).When tackling external environment and coercing,
Hide potential risk by avoidance behaviour to have found in most of biologies.Such as, Das
&Stickle (1994) finds, germling blue crab (Callinectes sapidus) and little blue crab (Callinectes
Similis) when sensing hypoxia water, show obvious avoidance behaviour, the most this hide row
For (shrimp) is also found in other shell-fish (Cook&Boyd 1965, Haefner
1971).Under whole world change background, water body physicochemical environment faces bigger change, and global warming causes
The rising of surface water temperature, the various Organic substance of water body, heavy metal concentration increase, water acidification etc.
The aquatile living in different water levels will be produced considerable influence.Aquatic zooplankton is as secondary
The significant contributor of the level productivity, at aspects such as food chain, food web and ecosystems services
Play a significant role.Under the change of water body physicochemical environment, what kind of behavior aquatic zooplankton has
Learning change is the problem in science merited attention.
But, in the prior art, respond varying environment about fast verification zooplankton
The relevant device of the Behavioral change of the factor or equivalent environment factor variable concentrations level is less, and real
Test equipment mechanism simple, it is impossible to competent high-level requirement of experiment, also lack relevant survey simultaneously
Examination specification.
Summary of the invention
1. to solve the technical problem that
It is an object of the invention to, it is provided that a kind of test water body chemical environment is to plankton
Experimental system and experimental technique, described experimental system and the method for behavioristics's impact can ensure
Experiment condition is accurate, and experiment effect is accurate, it is adaptable to fast verification zooplankton response different rings
The border factor or the Behavioral change of equivalent environment factor variable concentrations level.
2. technical scheme
The purpose of the present invention is achieved by following technical scheme.
A kind of test the experiment system that plankton behavioristics is affected by water body chemical environment
System, it includes laboratory table assembly, illumination adjustments assembly, temperature-controlled package, central authorities' control group
Part and uninterrupted power source assembly.Wherein, described uninterrupted power source assembly respectively with laboratory table assembly,
Illumination adjustments assembly, temperature-controlled package, control assembly with central authorities and be connected, is used for as each
Assembly is powered incessantly, described central authorities control assembly respectively with laboratory table assembly, illumination adjustments
Assembly, is connected with temperature-controlled package, for corresponding assembly is implemented centralized Control.
Described illumination adjustments assembly includes: be arranged in around described laboratory table assembly is multiple
Light source, light source driving circuit, and brightness sensing circuit.Wherein, the plurality of light source is with described
Light source driving circuit is connected, described light source driving circuit respectively with described central authorities control assembly and
Uninterrupted power source assembly is connected, and described luminance sensor circuit arrangement is at described laboratory table assembly
Around, and control assembly with described central authorities to be connected.Described central authorities control assembly and obtain described bright
The brightness data of degree sensor circuit, and bright be set in advance in that central authorities control in the middle of assembly
Degree desired value compares, and if above brightness desired value, then central authorities control described in assembly control
Light source driving circuit reduces power, and then reduces brightness, until it reaches brightness desired value, if
Less than brightness desired value, then central authorities control assembly and control described light source driving circuit raising power,
Until reaching brightness desired value, and it is always maintained at this brightness desired value.
Described temperature-controlled package includes: be arranged in around described laboratory table assembly is multiple
Air conditioning unit, relay circuit, and temperature sensor circuit.Wherein, the plurality of air conditioner
Group is connected with described relay circuit, described relay circuit respectively with described central control group
Part is connected with uninterrupted power source assembly, and described temperature sensor circuit is arranged in described laboratory table
Around assembly, and it is connected with the described central assembly that controls.Described central authorities control assembly and obtain institute
State the temperature data of temperature sensor circuit, and control in the middle of assembly with being set in advance in central authorities
Temperature desired value compare, if above temperature desired value, then central authorities control assembly control
Described relay circuit is connected air conditioning unit, and then reduces temperature, until it reaches temperature desired value,
If less than temperature desired value, then central authorities control assembly and control described relay circuit connection air-conditioning
Unit, and then rise high-temperature, until it reaches temperature desired value, and it is always maintained at the expection of this temperature
Value.
Described central authorities control assembly and include: host apparatus, display device, information input dress
Put, camera head and alarm device.Wherein, host apparatus respectively with display device, information is defeated
Entering device, camera head is connected with alarm device, and described host apparatus includes central authorities' process
Unit and data storage cell.
Described laboratory table assembly includes: multiple liquid storing bags, and multiple volume control devices are many
Bar tube for transfusion and test container.Wherein, each liquid storing bag all with a volume control device,
Article one, tube for transfusion and test container are sequentially connected and connect.The plurality of liquid storing bag store different physics and chemistry because of
The liquid of son.Described volume control device includes: valve body, piston/cylinder, piston piece, spring
Device, depression bar, and depression bar driving means.Wherein, piston piece can be reciprocal in piston/cylinder
Motion, described valve body lower portion is provided with outlet, and described outlet coordinates with piston piece.Described work
Plug member, depression bar, depression bar driving means and described central authorities control assembly are sequentially connected and connect.Described
Central authorities control assembly control under, depression bar driving means drive depression bar, and then adjust the mouth of a river with
Cooperation between piston piece, it is achieved the control to flow velocity.
Described test container includes: agent set, central inlet district, anti-escape catch net,
Selectivity test region, and multiple pieces of glass dividing plate.Wherein, described agent set is by circular non-opaque
Lucite tank is constituted, it apopore including being close to bottom of gullet.Described central inlet district
Being positioned at the position of described circular non-opaque lucite tank central authorities, it includes multiple water inlet, institute
State water inlet to be connected with described tube for transfusion.Described glass partition is that radial is uniformly arranged,
Thus tank is divided into multiple radiation areas.
Preferably, a diameter of 20cm of circular non-opaque lucite tank, described water inlet
The perforate of mouth is highly 2cm, a diameter of 10cm of described anti-escape catch net.Described glass every
The height of plate is 3cm.
Preferably, described depression bar driving means is motor.
Preferably, described polylith dividing plate can be arranged more than 3 pieces according to experiment test needs
Different masses number, thus tank is divided into the radiation areas of corresponding more than 3
Preferably, described test container has 8 pieces of glass partitions, is divided by test container
It is 8 radiation areas, 8 kinds of factors can be obtained in described 8 radiation areas or gradient processes.
Another aspect of the present invention provides the above-mentioned test water body chemical environment of use to swimming
The experimental system of biological behavioristics impact carries out testing water body chemical environment to plankton behavioristics
The experimental technique of impact, described experimental technique comprises below step:
The first step, carries out first group of preliminary experiment, gets out pretest water body in liquid storing bag,
Connect water inlet and liquid storing bag, in central authorities control assembly, regulate pretest go out water speed, until
Water velocity does not affect the regional water body that moves freely and respectively select of zooplankton can be at this flow velocity
Under completely separable, record this in the data store and go out water speed.
Second step, carries out second group of preliminary experiment, gets out pretest water body in liquid storing bag,
Connect water inlet and liquid storing bag, first group of preliminary experiment is set in central authorities control assembly and obtains water outlet
Speed, observes zooplankton and experiment processes the right times of response, and be recorded in data
In memory element.
3rd step, gets out official testing water body in multiple liquid storing bags, sets concentration
Gradient or factor pattern.In central authorities control assembly, brightness, temperature and water outlet speed data are set,
Keep the temperature of experimental system, brightness and to go out water speed constant.
4th step, the under the microscope single or multiple health of picking, active plankton,
It is placed in circular non-opaque lucite tank, opens the volume control device that each liquid storing bag is connected,
Liquid level remains 2-3cm.
5th step, determines selection preference, sensitivity.If testing with single individuality for test
Object, then record this individuality time of staying in each selection region as determining that it is to test factor
Preference.If testing with colony for test object, then record this colony and be distributed within the set time
In the ratio in each selection region, in this, as this test object preference to test factor.
3. beneficial effect
The beneficial effects of the present invention is, compared with prior art, study multiple or the most terraced
Under the conditions of degree physical and chemical factor, the response of planktonic behavioristics, overcomes the middle-size and small-size reality of prior art
Experiment device fails to consider to measure the change of plankton behavioristics under gradient multifactor, many, the most also
Lacking the defect that relevant test method carries out specification, the test device utilizing the present invention can be simple
Effectively carrying out above-mentioned experimentation, experiment material used is simple and easy to get, the persuasion of method of testing
Power and repeatability are higher, it is possible to for systematic study and the nature of hydroplankton physiological ecological
Scientific development provides reliable experimental basis.
Accompanying drawing explanation
Fig. 1 is a kind of test water body chemical ring constituted according to the preferred embodiment of the present invention
The border connection block diagram on the experimental system that plankton behavioristics affects.
Fig. 2 is a kind of test water body chemical ring constituted according to the preferred embodiment of the present invention
The border connection block diagram on the illumination adjustments assembly of the experimental system that plankton behavioristics affects.
Fig. 3 is a kind of test water body chemical ring constituted according to the preferred embodiment of the present invention
The border connection block diagram on the temperature-controlled package of the experimental system that plankton behavioristics affects.
Fig. 4 is a kind of test water body chemical ring constituted according to the preferred embodiment of the present invention
Border controls the connection block diagram of assembly to the central authorities of the experimental system that plankton behavioristics affects.
Fig. 5 is a kind of test water body chemical ring constituted according to the preferred embodiment of the present invention
The border structural representation on the volume control device of the experimental system that plankton behavioristics affects.
Fig. 6 is a kind of test water body chemical ring constituted according to the preferred embodiment of the present invention
The border structural representation on the test container of the experimental system that plankton behavioristics affects.
In figure: 1-valve body；2-piston piece；3-anti-escape catch net；4-dividing plate；In 5-
Centre inhalant region；6-circular non-opaque lucite tank；10-depression bar.
Detailed description of the invention
Hereinafter, with reference to accompanying drawing, the specific embodiment of the present invention will be retouched in detail
State, according to the description that these are detailed, one of ordinary skill in the art it can be clearly understood that the present invention,
And the present invention can be implemented.In the case of the principle of the invention, each different enforcement
Feature in example can be combined obtaining new embodiment, or substitutes some embodiment
In some feature, it is thus achieved that other is preferred embodiment.
Embodiment 1:
With reference to Fig. 1, embodiments of the invention 1 provide a kind of test water body chemical environment
Experimental system on the impact of plankton behavioristics, it includes laboratory table assembly, illumination adjustments group
Part, temperature-controlled package, central authorities control assembly and uninterrupted power source assembly, wherein, described not
Uninterruptible power assembly respectively with laboratory table assembly, illumination adjustments assembly, temperature-controlled package, and
Central authorities control assembly and are connected, and for powering incessantly for each assembly, described central authorities control
Assembly respectively with laboratory table assembly, illumination adjustments assembly, be connected with temperature-controlled package, use
In corresponding assembly is implemented centralized Control.
With reference to Fig. 2, described illumination adjustments assembly includes: be arranged in described laboratory table assembly
Multiple light supply apparatuses around, light source driving circuit, and brightness sensing circuit；Wherein, described
Multiple light sources are connected with described light source driving circuit, and described light source driving circuit is respectively with described
Central authorities control assembly and are connected with uninterrupted power source assembly, and described luminance sensor circuit arrangement exists
Around described laboratory table assembly, and it is connected with the described central assembly that controls；Described central authorities control
Assembly can obtain the brightness data of described luminance sensor circuit, and be set in advance in
The brightness desired value that centre controls in the middle of assembly compares, if above brightness desired value, then in
Centre controls assembly and controls described light source driving circuit reduction power, and then reduces brightness, until reaching
To brightness desired value, if less than brightness desired value, then central authorities control assembly and control described light source
Drive circuit improves power, until it reaches brightness desired value, and is always maintained at this brightness desired value.
With reference to Fig. 3, described temperature-controlled package includes: be arranged in described laboratory table assembly
Around multiple air conditioning unit, relay circuit, and temperature sensor circuit；Wherein, described
Multiple air conditioning unit and described relay circuits are connected, and described relay circuit is respectively with described
Central authorities control assembly and are connected with uninterrupted power source assembly, and described temperature sensor circuit is arranged in
Around described laboratory table assembly, and it is connected with the described central assembly that controls；Described central authorities control
Assembly can obtain the temperature data of described temperature sensor circuit, and be set in advance in
The temperature desired value that centre controls in the middle of assembly compares, if above temperature desired value, then in
Centre controls the assembly described relay circuit of control and connects air conditioning unit, and then reduces temperature, until
Reaching temperature desired value, if less than temperature desired value, then central authorities control to continue described in assembly control
Appliance circuit is connected air conditioning unit, and then rises high-temperature, until it reaches temperature desired value, and one
Straight this temperature desired value of holding.
With reference to Fig. 4, described central authorities control assembly and include: host apparatus, display device,
Message input device, camera head and alarm device；Wherein, host apparatus fills with display respectively
Putting, message input device, camera head is connected with alarm device, wraps in described host apparatus
Include CPU and data storage cell.
Described laboratory table assembly includes: multiple liquid storing bags, and multiple volume control devices are many
Bar tube for transfusion and test container；Wherein, each liquid storing bag all with a volume control device,
Article one, tube for transfusion and test container are sequentially connected and connect.
The plurality of liquid storing bag stores the liquid of different physical and chemical factor；Described flow-control fills
Put and include: valve body 1, piston/cylinder, piston piece 2, spring assembly, depression bar 10, and depression bar drives
Dynamic device；Wherein, piston piece 2 moves back and forth in piston/cylinder, and described valve body 1 bottom sets
Outlet, described outlet is had to coordinate with piston piece 2；Described piston piece 2, depression bar 10, pressure
Rod driving device and described central authorities control assembly are sequentially connected and connect；Assembly is controlled in described central authorities
Under control, depression bar driving means drives depression bar 10, and then adjusts between the mouth of a river and piston piece 2
Cooperation, it is achieved the control to flow velocity.
With reference to Fig. 5 and 6, described test container includes: agent set, central inlet district
5, anti-escape catch net 3, selectivity test region, and multiple pieces of glass dividing plate 4；Wherein, institute
Stating agent set to be made up of the circular non-opaque lucite tank 10 of a diameter of 20cm, it includes
It is close to the apopore bottom tank 10；It is organic that described central inlet district 5 is positioned at described circular non-opaque
The position of glass flume 10 central authorities, it includes multiple water inlet, the perforate height of described water inlet
For 2cm, described water inlet is connected with described tube for transfusion；Described anti-escape catch net 3 straight
Footpath is 10cm；The height of described glass partition 4 is 3cm, is uniformly arranged in radial, from
And tank 10 is divided into multiple radiation areas, can obtain in the plurality of radiation areas multiple because of
Element or gradient process.
Embodiment 2:
Embodiments of the invention 2 provide a kind of experimental system used in embodiment 1 and enter
The experimental technique that plankton behavioristics is affected by row test water body chemical environment, described experiment side
Method comprises below step:
(1) carry out first group of preliminary experiment, liquid storing bag be ready to pretest water body,
Connect water inlet and liquid storing bag, in central authorities control assembly, regulate pretest go out water speed, until
Water velocity does not affect the regional water body that moves freely and respectively select of zooplankton can be at this flow velocity
Under completely separable, record this in the data store and go out water speed.
(2) carry out second group of preliminary experiment, liquid storing bag be ready to pretest water body,
Connect water inlet and liquid storing bag, first group of preliminary experiment is set in central authorities control assembly and obtains water outlet
Speed, observes zooplankton and experiment processes the right times of response, and be recorded in data
In memory element.
(3) in multiple liquid storing bags, get out official testing water body, set concentration ladder
Degree or factor pattern；In central authorities control assembly, brightness, temperature and water outlet speed data are set,
Keep the temperature of experimental system, brightness and to go out water speed constant.
(4) the single or multiple health of picking, active plankton under the microscope,
It is placed in the circular channels 10 shown in Fig. 6, opens the volume control device that each liquid storing bag is connected,
Liquid level remains 2-3cm.
(5) selection preference, sensitivity are determined.If it is right to test with single individuality for test
As, then record this individuality time of staying in each selection region as determining that it is to test factor
Preference；If testing with colony for test object, then record this colony and be distributed within the set time
Each ratio selecting region, in this, as this test object preference to test factor.
Embodiment 3:
Embodiments of the invention 3 are the further improvement to embodiment 1, described polylith every
Plate 4 can arrange the different masses number more than 3 pieces according to experiment test needs, thus by tank 10
It is divided into the radiation areas of corresponding more than 3.
Embodiment 4:
Embodiments of the invention 4 are the further improvement to embodiment 1, and described test is held
Utensil has 8 pieces of glass partitions 4, i.e. test container is divided into 8 radiation areas, and described 8
8 kinds of factors can be obtained in individual radiation areas or gradient processes, determine 8 selectivity test district water
Body is completely separable can use the solution not allowing color to carry out preliminary experiment, such as potassium permanganate solution
(aubergine), pure water (colourless), ferrum sulfuricum oxydatum solutum etc..
The beneficial effects of the present invention is, compared with prior art, study multiple or the most terraced
Under the conditions of degree physical and chemical factor, the response of planktonic behavioristics, overcomes the middle-size and small-size reality of prior art
Experiment device fails to consider to measure the change of plankton behavioristics under gradient multifactor, many, the most right
Lacking the defect to relevant test method specification, the test device utilizing the present invention can be the most effective
Carry out above-mentioned experimentation, experiment material used is simple and easy to get, the cogency of method of testing and
Repeatability is higher, it is possible to for systematic study and the natural science of hydroplankton physiological ecological
Development provides reliable experimental basis.
Although above by referring to specific embodiment, invention has been described, but
One of ordinary skill in the art should be appreciated that in principle disclosed by the invention and scope, can be with pin
Configuration disclosed by the invention and details are made many amendments.Protection scope of the present invention is by appended
Claim determine, and claim be intended to technical characteristic in claim etc.
Whole amendments included by jljl literal meaning or scope.
Claims (6)
1. test the experimental system that plankton behavioristics is affected by water body chemical environment, its bag
Include laboratory table assembly, illumination adjustments assembly, temperature-controlled package, central authorities control assembly and not between
Power-off source component；Wherein, described uninterrupted power source assembly respectively with laboratory table assembly, illumination adjust
Joint assembly, temperature-controlled package, with central authorities control assembly and are connected, for for each assembly not
Power discontinuously, described central authorities control assembly respectively with laboratory table assembly, illumination adjustments assembly,
It is connected with temperature-controlled package, for corresponding assembly is implemented centralized Control；
It is characterized in that,
Described illumination adjustments assembly includes: be arranged in the multiple light sources around described laboratory table assembly,
Light source driving circuit, and brightness sensing circuit；Wherein, the plurality of light source drives with described light source
Galvanic electricity road is connected, and described light source driving circuit controls assembly and uninterruptedly respectively with described central authorities
Power supply module is connected, described luminance sensor circuit arrangement around described laboratory table assembly,
And be connected with the described central assembly that controls；Described central authorities control assembly and obtain described brightness sensing
The brightness data of device circuit, and control the brightness expection in the middle of assembly with being set in advance in central authorities
Value compares, and if above brightness desired value, then central authorities' control assembly described light source of control drives
Galvanic electricity road reduces power, and then reduces brightness, until it reaches brightness desired value, if less than bright
Degree desired value, then central authorities control assembly and control described light source driving circuit raising power, until reaching
To brightness desired value, and it is always maintained at this brightness desired value；
Described temperature-controlled package includes: be arranged in the multiple air-conditionings around described laboratory table assembly
Unit, relay circuit, and temperature sensor circuit；Wherein, the plurality of air conditioning unit with
Described relay circuit is connected, described relay circuit respectively with described central authorities control assembly and
Uninterrupted power source assembly is connected, and described temperature sensor circuit is arranged in described laboratory table assembly
Around, and control assembly with described central authorities to be connected；Described central authorities control assembly and obtain described temperature
The temperature data of degree sensor circuit, and control the temperature in the middle of assembly with being set in advance in central authorities
Degree desired value compares, and if above temperature desired value, then central authorities control described in assembly control
Relay circuit is connected air conditioning unit, and then reduces temperature, until it reaches temperature desired value, as
Fruit is less than temperature desired value, then air conditioner connected by central authorities' control assembly described relay circuit of control
Group, and then rise high-temperature, until it reaches temperature desired value, and it is always maintained at this temperature desired value；
Described central authorities control assembly and include: host apparatus, display device, message input device,
Camera head and alarm device；Wherein, host apparatus respectively with display device, information input dress
Putting, camera head is connected with alarm device, and described host apparatus includes CPU
And data storage cell；
Described laboratory table assembly includes: multiple liquid storing bags, and multiple volume control devices are a plurality of defeated
Liquid pipe and test container；Wherein, each liquid storing bag all with a volume control device, one
Tube for transfusion and test container are sequentially connected and connect；The plurality of liquid storing bag stores different physical and chemical factor
Liquid；Described volume control device includes: valve body, piston/cylinder, piston piece, spring assembly,
Depression bar, and depression bar driving means；Wherein, piston piece can move back and forth in piston/cylinder,
Described valve body lower portion is provided with outlet, and described outlet coordinates with piston piece；Described piston piece,
Depression bar, depression bar driving means and described central authorities control assembly are sequentially connected and connect；In the control of described central authorities
Under the control of assembly processed, depression bar driving means drives depression bar, and then adjusts the mouth of a river and piston piece
Between cooperation, it is achieved the control to flow velocity；
Described test container includes: agent set, central inlet district, anti-escape catch net, choosing
Selecting property test zone, and multiple pieces of glass dividing plate；Wherein, described agent set is had by circular non-opaque
Machine glass flume is constituted, it apopore including being close to bottom of gullet；Described central inlet position
In the position of described circular non-opaque lucite tank central authorities, it includes multiple water inlet, described
Water inlet is connected with described tube for transfusion；Described glass partition is that radial is uniformly arranged, from
And tank is divided into multiple radiation areas.
A kind of test water body chemical environment the most according to claim 1 is to plankton behavior
Learn the experimental system of impact, it is characterised in that circular non-opaque lucite tank a diameter of
20cm, the perforate of described water inlet is highly 2cm, described anti-escape catch net a diameter of
10cm；The height of described glass partition is 3cm.
A kind of test water body chemical environment the most according to claim 1 is to plankton behavior
Learn the experimental system of impact, it is characterised in that described depression bar driving means is motor.
A kind of test water body chemical environment the most according to claim 1 is to plankton behavior
Learn impact experimental system, it is characterised in that described polylith dividing plate can according to experiment test needs,
Different masses number more than 3 pieces is set, thus tank is divided into the radiation of corresponding more than 3
Region
A kind of test water body chemical environment the most according to claim 4 is to plankton behavior
Learn the experimental system of impact, it is characterised in that described test container has 8 pieces of glass partitions,
Test container is divided into 8 radiation areas, can obtain in described 8 radiation areas 8 kinds because of
Element or gradient process.
6. a kind of test water body chemical environment pair used according to any one of claim 1-5
The experimental system of plankton behavioristics impact carries out testing water body chemical environment to plankton row
For learning the experimental technique of impact, it is characterised in that
Described experimental technique comprises below step:
The first step, carries out first group of preliminary experiment, gets out pretest water body, connect in liquid storing bag
Logical water inlet and liquid storing bag, regulate pretest in central authorities control assembly and go out water speed, until water
Flow Velocity does not affect the regional water body that moves freely and respectively select of zooplankton can be under this flow velocity
Completely separable, record this in the data store and go out water speed；
Second step, carries out second group of preliminary experiment, gets out pretest water body, connect in liquid storing bag
Logical water inlet and liquid storing bag, arrange first group of preliminary experiment in central authorities control assembly and obtain water outlet speed
Degree, observes zooplankton and experiment processes the right times of response, and be recorded in data and deposit
In storage unit；
3rd step, gets out official testing water body in multiple liquid storing bags, sets Concentraton gradient
Or factor pattern；Brightness, temperature and water outlet speed data are set in central authorities control assembly, protect
Hold the temperature of experimental system, brightness and to go out water speed constant；
4th step, the single or multiple health of picking, active plankton, put under the microscope
In circular non-opaque lucite tank, open the volume control device that each liquid storing bag is connected, liquid
Face height remains 2-3cm；
5th step, determines selection preference, sensitivity；If testing with single individuality for test object,
Then record this individuality time of staying in each selection region as determining its inclined to test factor
Good；If testing with colony for test object, then record this colony and be distributed within the set time respectively
Select the ratio in region, in this, as this test object preference to test factor.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290771A (en) * | 2016-09-30 | 2017-01-04 | 中国科学院合肥物质科学研究院 | The detection device of nano material pollutant system biological effect and application |
| CN114617098A (en) * | 2022-03-28 | 2022-06-14 | 河南省科学院地理研究所 | Experimental device for testing influence of water environment factors on plankton behaviourology |
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| CN105301212A (en) * | 2014-07-04 | 2016-02-03 | 中国环境科学研究院 | High-throughput fish ecotoxicity test system |
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| CN101542281A (en) * | 2007-06-08 | 2009-09-23 | 株式会社百奥尼 | Measuring equipment for quality of water |
| CN102144581A (en) * | 2011-01-23 | 2011-08-10 | 三峡大学 | Fish functional ethology device |
| CN103487561A (en) * | 2013-09-17 | 2014-01-01 | 河海大学 | Early warning device and method for identifying sudden water pollution based on biotic population module |
| CN203897053U (en) * | 2014-05-04 | 2014-10-29 | 上海海洋大学 | Experiment device for research of biological behaviors |
| CN105301212A (en) * | 2014-07-04 | 2016-02-03 | 中国环境科学研究院 | High-throughput fish ecotoxicity test system |
| CN204086271U (en) * | 2014-09-28 | 2015-01-07 | 黄山学院 | A kind ofly study the proving installation of ultraviolet spectrum on the ethological impact of planktonic organism |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106290771A (en) * | 2016-09-30 | 2017-01-04 | 中国科学院合肥物质科学研究院 | The detection device of nano material pollutant system biological effect and application |
| CN106290771B (en) * | 2016-09-30 | 2018-06-29 | 中国科学院合肥物质科学研究院 | The detection device of nano material-pollution objects system biological effect and application |
| CN114617098A (en) * | 2022-03-28 | 2022-06-14 | 河南省科学院地理研究所 | Experimental device for testing influence of water environment factors on plankton behaviourology |
| CN114617098B (en) * | 2022-03-28 | 2023-10-24 | 河南省科学院地理研究所 | Experimental device for testing influence of water body environmental factors on plankton behaviours |
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