CN106290347A - The manufacture method of a kind of variable color reagent paper detecting beef system waterpower and detection method - Google Patents
The manufacture method of a kind of variable color reagent paper detecting beef system waterpower and detection method Download PDFInfo
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- CN106290347A CN106290347A CN201610901080.7A CN201610901080A CN106290347A CN 106290347 A CN106290347 A CN 106290347A CN 201610901080 A CN201610901080 A CN 201610901080A CN 106290347 A CN106290347 A CN 106290347A
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Abstract
The invention belongs to meat sample quality detection technique field, relate to manufacture method and the detection method of a kind of variable color reagent paper detecting beef system waterpower.Solve that prior art operation is complicated, it is long to process the time, it is impossible to well carry out on-line checking beef system hydraulic techniques problem.Manufacture method comprises the following steps: 1, weighs 2~3g cobaltous chloride reagent and dissolves in 20ml deionized water;2, the reagent paper of preparation be impregnated in the cobalt chloride solution configured 12~18min;3, blot, with absorbent paper, the reagent that reagent paper surface is not to be adsorbed, be placed in culture dish, in drying baker 15~20min;Detection method comprises the following steps: 1, cut along being perpendicular to muscle fiber direction by beef sample;2, variable color reagent paper is attached to beef sample surface;3, dry microscope slide is placed on variable color reagent paper, presses lightly on;4, reagent paper discoloration is observed through microscope slide;5, according to reagent paper Coloring Time, regression equation is utilized to judge beef system waterpower size.
Description
Technical field
The invention belongs to meat sample quality detection technique field, particularly relate to a kind of variable color examination detecting beef system waterpower
The manufacture method of paper and detection method.
Background technology
Beef is as the meat products of a kind of rich in proteins, low fat, cellulose and mineral, and it enjoys " proud in meat
Son " laudatory title, deeply liked by consumers in general.In China, along with the raising of people's living standard, beef consumption figure constantly increases
Long, meanwhile, along with people are to healthy growing interest, consumer is more and more higher to the requirement of beef quality.Beef
Quality includes all many-sides, such as yellowish pink, tenderness, marbling, local flavor, taste quality etc..Wherein, beef is waterpower quality
Also be the importance of beef quality detection, be the height of waterpower be directly connected to the quality of beef product, tenderness, section,
Multinomial quality index and the economic indicators such as elasticity, mouthfeel, yield rate, determine beef segmentation, freezing, preserve and processing etc. one
Weight loss in serial procedures and the quality of converted products, be the index with great economy meaning.Therefore, beef system water
The detection of power is particularly important in Beef production.At present, the method for domestic and international beef system waterpower Quality Detection mainly pressure
Force method, drip loss method, take broken rate method, cooking loss method etc., due to exist waste time and energy, detection equipment is the most portative lacks
Point, therefore needs proposition badly a kind of accurately, and quickly, simple and easy to do is hydraulic detection method.
Summary of the invention
Present invention seek to address that prior art operation is complicated, it is long to process the time, it is impossible to well carry out on-line checking beef
It is the technical problems such as waterpower, and provides a kind of and beef system waterpower can be realized variable color reagent paper economic, quickly detection.
For solving above-mentioned technical problem, the present invention adopts the following technical scheme that realization:
The manufacture method of a kind of variable color reagent paper detecting beef system waterpower, comprises the following steps:
Step 1: configuration cobalt chloride solution, weighs 2~3g cobaltous chloride reagent and dissolves in 20ml deionized water, stirring mixing
Uniformly;
Step 2: dipping reagent paper, impregnated in the cobalt chloride solution configured 12~18min by the reagent paper of preparation;
Step 3: dry reagent paper, after reaching dip time, reagent paper tweezers are taken out from cobalt chloride solution, uses absorbent paper
Blot the reagent that reagent paper surface is not to be adsorbed, be placed in culture dish, in drying baker 15~20min, obtain variable color reagent paper.
In technical scheme steps 2, method for preparing test paper is as follows:
Select the quantitative filter paper of Ф 7cm, cutting growth × a width of 5 × 1cm reagent paper.
The temperature that arranges of drying baker described in technical scheme is 65~75 DEG C.
The manufacture method of described a kind of variable color reagent paper detecting beef system waterpower, also includes step 4: to described in step 3
Variable color reagent paper be packaged, load the variable color reagent paper of drying to seal in aluminium foil bag together with desiccant and preserve, it is to avoid hands is straight
The reagent paper that contact is processed, should wear glove and operate, be placed in dry environment storage, standby.
The detection method of a kind of variable color reagent paper detecting beef system waterpower, comprises the following steps:
Step 1: beef sample is cut along being perpendicular to muscle fiber direction;
Step 2: with tweezers, variable color reagent paper is attached to the beef sample surface cut;
Step 3: drier microscope slide is immediately placed on variable color reagent paper, and presses lightly on;
Step 4: observe reagent paper discoloration through microscope slide, record variable color reagent paper is become pink from blueness and is taken
Between;
Step 5: according to reagent paper Coloring Time, utilizes regression equation to judge beef system waterpower size.
In technical scheme steps 1 beef sample choose and process by following standard:
Test beef sample requires that above brain position is test sample, rejects the muscle of fresh beef sample, tendon, film, fat, only
Retain muscle fiber part.
The method that in technical scheme steps 5, regression equation obtains comprises the following steps:
(51) utilize National Standard Method that experiment beef sample system waterpower is measured;
(52) beef sample variable color required time in variable color detection paper (51) is utilized;
(53) with y for being waterpower, x is reagent paper Coloring Time, uses SPSS software to enter the measured value in (51) and (52)
Asking for of row Linear Regression Model in One Unknown;
(54) the regression equation y=20.979+1.307x being waterpower with reagent paper Coloring Time is drawn.
Utilizing National Standard Method to be measured experiment beef sample system waterpower in technical scheme, concrete operations are with reference to such as subscript
Accurate:
(1) determination of water:
The first method according in GB-5009.3-2010 " mensuration of moisture in national food safety standard food ": directly do
Dry method is measured;
(2) percentage of water loss measures:
It is measured according to NYT 1333-2007 " mensuration of animal meat quality ";
(3) it is that waterpower measures
Utilizing following equation to calculate is waterpower: be waterpower (%)=(moisture content-percentage of water loss)/moisture content × 100%
Compared with prior art the invention has the beneficial effects as follows:
Manufacture method and the detection method of the variable color reagent paper of detection the most of the present invention beef system waterpower solve tradition
The drawback of method detection beef system waterpower quality, i.e. time and effort consuming, cost is high, detection means is loaded down with trivial details restriction, this detection method
Easy, it is easy to operation.
Manufacture method and the detection method of the variable color reagent paper of detection the most of the present invention beef system waterpower can conveniently realize
Detection beef is the Changing Pattern of waterpower during depositing, and for beef is that waterpower evaluation provides one more objectively side
Method.
Manufacture method and the detection method of the variable color reagent paper of detection the most of the present invention beef system waterpower achieve cattle
Meat system waterpower quality quick, easy, detect accurately, have the strongest practicality, can accomplish save the detection time and reduce warp
Ji puts into the effect with manual labor.
Accompanying drawing explanation
Fig. 1 is the manufacture method FB(flow block) of the variable color reagent paper of detection of the present invention beef system waterpower.
Fig. 2 is the detection method FB(flow block) of the variable color reagent paper of detection of the present invention beef system waterpower.
Detailed description of the invention
The invention will be further elaborated below:
One, the present invention provides the manufacture method of a kind of variable color reagent paper, comprises the following steps:
Step 1: configuration cobalt chloride solution, weighs 2~3g cobaltous chloride reagent and dissolves in 20ml deionized water, stirring mixing
Uniformly;
Step 2: dipping reagent paper, impregnated in the cobalt chloride solution configured 12~18min by the reagent paper of preparation;
Step 3: dry reagent paper, after reaching dip time, reagent paper tweezers are taken out from cobalt chloride solution, uses absorbent paper
Blotting the reagent that reagent paper surface is not to be adsorbed, be placed in culture dish, in drying baker, 15~20min (arranging drying baker temperature is
65~75 DEG C), obtain variable color reagent paper;
Step 4: encapsulation variable color reagent paper, seals in the variable color reagent paper of drying loads together with desiccant aluminium foil bag and preserves
(avoiding hands directly to contact processed reagent paper, should wear glove and operate), is placed in dry environment storage, standby;
Two, the present invention also provides for the detection method of a kind of variable color reagent paper, comprises the following steps:
Step 1: beef sample is cut along being perpendicular to muscle fiber direction;
Step 2: with tweezers, variable color reagent paper is attached to the beef sample surface cut;
Step 3: drier microscope slide is immediately placed on variable color reagent paper, and presses lightly on;
Step 4: observe reagent paper discoloration through microscope slide, record variable color reagent paper is become pink from blueness and is taken
Between;
Step 5: according to reagent paper Coloring Time, utilizes regression equation to judge beef system waterpower size.
Further, in step 1 beef sample choose and process by following standard:
Test beef sample requires that above brain position is test sample, rejects the muscle of fresh beef sample, tendon, film, fat etc.,
Only retain muscle fiber part.
Further, the method that in step 5, regression equation obtains comprises the following steps:
(51) utilize National Standard Method that experiment beef sample system waterpower is measured;
(52) beef sample variable color required time in variable color detection paper (51) is utilized;
(53) with y for being waterpower, x is reagent paper Coloring Time, uses SPSS software to enter the measured value in (51) and (52)
Asking for of row Linear Regression Model in One Unknown;
(54) regression equation being waterpower with reagent paper Coloring Time is drawn.
Further, the present invention utilizes National Standard Method to be determined experiment beef sample system waterpower, concrete operations reference
Following standard:
1. determination of water
Straight according to the first method in " mensuration of moisture in national food safety standard food " (GB-5009.3-2010)
Connect seasoning to be measured.
2. percentage of water loss measures
It is measured according to " mensuration of animal meat quality " (NYT 1333-2007).
3. it is that waterpower measures
Utilizing following equation to calculate is waterpower: be waterpower (%)=(moisture content-percentage of water loss)/moisture content × 100%
Three, the manufacture method of the variable color reagent paper of detailed description below detection beef system waterpower:
Embodiment 1: the manufacture method of the variable color reagent paper of detection beef system waterpower
Step 1: configuration cobalt chloride solution, weighs 2g cobaltous chloride reagent and dissolves in 20ml deionized water, stir with Glass rod
Mix and make its mix homogeneously;
Step 2: dipping reagent paper, impregnated in 12min in the cobalt chloride solution configured by the reagent paper of preparation;
Step 3: dry reagent paper, after reaching dip time, reagent paper tweezers are taken out from cobalt chloride solution, uses absorbent paper
Blotting the reagent that reagent paper surface is not to be adsorbed, be placed in culture dish, in drying baker, (arrange drying baker temperature is 65 to 15min
DEG C), obtain variable color reagent paper;
Step 4: encapsulation variable color reagent paper, seals in the variable color reagent paper of drying loads together with desiccant aluminium foil bag and preserves
(avoiding hands directly to contact processed reagent paper, should wear glove and operate), is placed in dry environment storage, standby;
Embodiment 2: the manufacture method of the variable color reagent paper of detection beef system waterpower
Step 1: configuration cobalt chloride solution, weighs 3g cobaltous chloride reagent and dissolves in 20ml deionized water, stir with Glass rod
Mix and make its mix homogeneously;
Step 2: dipping reagent paper, impregnated in 18min in the cobalt chloride solution configured by the reagent paper of preparation;
Step 3: dry reagent paper, after reaching dip time, reagent paper tweezers are taken out from cobalt chloride solution, uses absorbent paper
Blotting the reagent that reagent paper surface is not to be adsorbed, be placed in culture dish, in drying baker, (arrange drying baker temperature is 75 to 20min
DEG C), obtain variable color reagent paper;
Step 4: encapsulation variable color reagent paper, seals in the variable color reagent paper of drying loads together with desiccant aluminium foil bag and preserves
(avoiding hands directly to contact processed reagent paper, should wear glove and operate), is placed in dry environment storage, standby;
Further, step 2 pilot scale paper manufacture method is as follows:
Select Ф 7cm quantitative filter paper (middling speed) by test requirements document cutting growth × a width of 5 × 1cm reagent paper.
As a kind of preferred implementation, the most described filter paper is Medium speed filter paper, in other embodiments, also
Other kinds of filter paper can be used.
Four, the detection method of the variable color reagent paper of detection beef system waterpower:
Step 1: beef sample is cut along being perpendicular to muscle fiber direction;
Step 2: with tweezers, variable color reagent paper is attached to the beef sample surface cut;
Step 3: dry microscope slide is placed on variable color reagent paper, and presses lightly on;
Step 4: observe reagent paper discoloration through microscope slide, record variable color reagent paper is become pink from blueness and is taken
Between.
Further, in step 1 beef sample choose and process by following standard:
Test beef sample requires all to choose the bright moon beef in large supermarket, and above brain position is test sample, rejects new
The muscle of fresh beef sample, tendon, film, fat etc., only retain muscle fiber part;
The judgement of detection paper result:
Step 1: utilize National Standard Method that experiment beef sample system waterpower is measured;
Step 2: utilize beef sample variable color required time in variable color detection paper step 1;
Step 3: with y for being waterpower, x is reagent paper Coloring Time, uses SPSS soft measured value in step 1 and step 2
Part carries out asking for of Linear Regression Model in One Unknown;
Step 4: draw to be the waterpower Linear Regression Model in One Unknown corresponding with reagent paper Coloring Time.
Further, utilizing National Standard Method to be determined beef sample system waterpower, concrete operations are with reference to following standard:
1. determination of water
Straight according to the first method in " mensuration of moisture in national food safety standard food " (GB-5009.3-2010)
Connect seasoning to be measured.
1.1. direct drying method principle:
Utilize the physical property of moisture in food, at 101.3kPa (atmospheric pressure), adopt at temperature 101 DEG C~105 DEG C
The weight being dried less loss in sample is measured, including hygroscopic moisture, partially crystallizable water and the thing that can volatilize under the conditions of this by evaporation method
Matter, then the content of moisture is gone out by the weighing numerical computations before and after being dried.
1.2. reagent and material
Unless otherwise prescribed, in this method, agents useful for same is analytical pure.
1.2.1. hydrochloric acid: top grade is pure.
1.2.2. sodium hydroxide (NaOH): top grade is pure.
1.2.3. hydrochloric acid solution (6mol/L): measure 50mL hydrochloric acid, be diluted with water to 100mL.
1.2.4. sodium hydroxide solution (6mol/L): weigh 24g sodium hydroxide, is dissolved in water and is diluted to 100mL.
1.2.5. sea sand: take washing and remove sea sand or the fluvial sand of earth, first boil 0.5h with hydrochloric acid (1.2.3.), wash with water
To neutral, then boil 0.5h with sodium hydroxide solution (1.2.4.), wash with water to neutrality, through 105 DEG C of drying for standby.
2. instrument and equipment
2.1. flat aluminum or glass weighing botle.
2.2. Constant Temp. Oven.
2.3. exsiccator: included efficient drying agent.
2.4. balance: sensibility reciprocal is 0.1mg.
3. analytical procedure
Take the weighing botle of cleaning, inside add 10g sea sand and a little glass rod, be placed in 101 DEG C~105 DEG C of drying baker, be dried
Take out after 1.0h, weigh after putting into exsiccator cooling 0.5h, and repeat to be dried to constant weight.Then 5g~10g sample (essence is weighed
Really to 0.0001g), it is placed in evaporating dish, stirs evenly to be placed on boiling water bath with little glass rod and be evaporated, and stir at any time, wipe at the bottom of ware
Water droplet, puts and builds taking-up after being dried 4h in 101 DEG C~105 DEG C of drying baker, puts into weighing after exsiccator cooling 0.5h.The most again
Put into and 101 DEG C~105 DEG C of drying baker are dried about 1h, take out, put into after exsiccator cools down 0.5h and weigh again.And repeat with
Upper operation is of poor quality less than 2mg to front and back twice, is constant weight.
Note: twice constant weight value, in last calculating, takes last weighing value.
4. result calculates
Moisture in sample is calculated by (1) formula:
X1=(m1-m2)/(m1-m3)*100 (1)
In formula:
X1: the moisture in sample, unit is gram every hectogram (g/100g);
m1: weighing botle (adding sea sand, glass rod) and the quality of sample, unit is gram (g);
m2: the quality after weighing botle (adding sea sand, glass rod) and samples dried, unit is gram (g);
m3: the quality of weighing botle (adding sea sand, glass rod), unit is gram (g).
5. percentage of water loss measures
It is measured according to " mensuration of animal meat quality " (NYT 1333-2007).
5.1. sampling
Taking the longissimus dorsi muscle one section that thickness is 5cm from postmortem aging trunk, wherein cattle takes longissimus dorsi muscle the 12nd to the 13rd
Between lumbar vertebra.Using biplate cutter to be perpendicular to muscle fiber direction and cut the thick sliced meat of 1.0cm, horizontal, on clean rubber tissue, is used
(area of a circle is about 5cm to the sharp rounded sample device of a diameter of 2.523cm2), cut one piece of centre sample.
5.2. measure
The sample of above-mentioned acquirement is positioned on the balance that sensing amount is 0.001g immediately and weighs.After weighing, meat sample is placed in
Between two-layer gauze, gauze respectively pads the qualitative Medium speed filter paper of multilamellar, does not appears with moisture, and all exhaustion is degree, two-layer duroplasts
Between plate, it is placed in the soil of improvement and allows to expand and be forced into 35kg on compressometer platform, keep 5min, remove after pressure immediately from yarn
Cloth strips out meat sample weigh.Separately take two samples by the method for 2.1. simultaneously, repeat this step.
5.3. the mensuration of meat sample water content
Separately adopt meat sample 50g at same position, measure meat sample water content by the method for GB 5009.3 regulation.
5.4. the calculating of pressure application percentage of water loss
X2=m1A-(m1-m2)/m1A*100 (2)
In formula:
X2: pressure application percentage of water loss, %;
A: the water content in former muscle, %;
m1: meat sample weight before pressurization, g;
m2: meat sample weight, g after pressurization;
5.5. the determination of pressure application percentage of water loss
Obtain 3 numerical value are taken arithmetic average and is the pressure application percentage of water loss of meat sample.
6. it is that waterpower measures
Utilizing following equation to calculate is waterpower: be waterpower (%)=(moisture content-percentage of water loss)/moisture content × 100%
Building reagent paper Coloring Time and the regression equation of beef sample system waterpower, step is as follows:
Step 1: measure 10 groups of difference beef samples and make the time x of reagent paper variable color (being shown in Table 1, every kind of meat sample does three parallel examinations
Test);
Step 2: measure 10 groups of difference beef samples is waterpower y (being shown in Table 1, every kind of meat sample does three parallel tests);
Step 3: with table 1 test data as sample, uses SPSS software to carry out asking for of Linear Regression Model in One Unknown.Obtain
The linear regression equation of y (being waterpower) and x (reagent paper Coloring Time) is:
Y=20.979+1.307x (3)
Testing equation (3) significance, F value is 220.208, looks into F table and obtains F0.05(1,8)=5.32, F0.01(1,8)=
11.26, F=220.208 > F0.01(1,8)=11.26, P < 0.01, so F value reaches highly significant level, show have between y and x
Having highly significant linear relationship, regression beeline equation is effective.Relation between beef system waterpower and reagent paper Coloring Time can use y
=20.979+1.307x describes.
Table 1 meat sample system's waterpower (y) and reagent paper Coloring Time (x) measurement result
Utilizing National Standard Method checking reagent paper to measure the accuracy of beef system waterpower, step is as follows:
Step 1: measure 10 groups of difference beef samples and make the time x of test strips variable color (table 2, every kind of meat sample does three parallel examinations
Test);
Step 2: the x value recorded is substituted in the linear regression equation set up, tries to achieve y value;
Step 3: measure 10 groups of difference beef samples is waterpower Y (table 2, every kind of meat sample does three parallel tests);
Step 4: compare y and Y, by table 2, the 9th group of ELISA test strip overlong time, y value is bigger than normal, there is detection error, therefore
The accuracy rate of detection paper beef system waterpower is 90%.
Table 2 checking test comparative result
Claims (8)
1. the manufacture method of the variable color reagent paper detecting beef system waterpower, it is characterised in that comprise the following steps:
Step 1: configuration cobalt chloride solution, weighs 2~3g cobaltous chloride reagent and dissolves in 20ml deionized water, and stirring mixing is all
Even;
Step 2: dipping reagent paper, impregnated in the cobalt chloride solution configured 12~18min by the reagent paper of preparation;
Step 3: dry reagent paper, after reaching dip time, reagent paper tweezers are taken out from cobalt chloride solution, blots with absorbent paper
The reagent that reagent paper surface is not to be adsorbed, is placed in culture dish, in drying baker 15~20min, obtains variable color reagent paper.
2. the manufacture method of the variable color reagent paper of beef system waterpower is detected according to claim 1 one kind, it is characterised in that:
In step 2, method for preparing test paper is as follows:
Select the quantitative filter paper of Ф 7cm, cutting growth × a width of 5 × 1cm reagent paper.
The manufacture method of a kind of variable color reagent paper detecting beef system waterpower the most according to claim 1, it is characterised in that:
The temperature that arranges of described drying baker is 65~75 DEG C.
The manufacture method of a kind of variable color reagent paper detecting beef system waterpower the most according to claim 1, it is characterised in that:
Also include step 4: the variable color reagent paper described in step 3 is packaged, the variable color reagent paper of drying is filled together with desiccant
Seal in entering aluminium foil bag and preserve, it is to avoid hands directly contacts processed reagent paper, should wear glove and operate, be placed in dry environment
Store, standby.
5. the detection method of the variable color reagent paper of detection beef system as claimed in claim 1 waterpower, it is characterised in that include following
Step:
Step 1: beef sample is cut along being perpendicular to muscle fiber direction;
Step 2: with tweezers, variable color reagent paper is attached to the beef sample surface cut;
Step 3: drier microscope slide is immediately placed on variable color reagent paper, and presses lightly on;
Step 4: observe reagent paper discoloration through microscope slide, record variable color reagent paper is become pink required time from blueness;
Step 5: according to reagent paper Coloring Time, utilizes regression equation to judge beef system waterpower size.
The detection method of the variable color reagent paper of detection the most according to claim 5 beef system waterpower, it is characterised in that:
In step 1 beef sample choose and process by following standard:
Test beef sample requires that above brain position is test sample, rejects the muscle of fresh beef sample, tendon, film, fat, only retains
Muscle fiber part.
The detection method of the variable color reagent paper of detection the most according to claim 5 beef system waterpower, it is characterised in that:
The method that in step 5, regression equation obtains comprises the following steps:
(51) utilize National Standard Method that experiment beef sample system waterpower is measured;
(52) beef sample variable color required time in variable color detection paper (51) is utilized;
(53) with y for being waterpower, x is reagent paper Coloring Time, uses SPSS software to carry out one the measured value in (51) and (52)
Asking for of unit's linear regression model (LRM);
(54) the regression equation y=20.979+1.307x being waterpower with reagent paper Coloring Time is drawn.
The detection method of the variable color reagent paper of detection the most according to claim 7 beef system waterpower, it is characterised in that:
Utilizing National Standard Method to be measured experiment beef sample system waterpower, concrete operations are with reference to following standard:
(1) determination of water:
The first method according in GB-5009.3-2010 " mensuration of moisture in national food safety standard food ": direct drying method
It is measured;
(2) percentage of water loss measures:
It is measured according to NYT 1333-2007 " mensuration of animal meat quality ";
(3) it is that waterpower measures:
Utilizing following equation to calculate is waterpower: be waterpower (%)=(moisture content-percentage of water loss)/moisture content × 100%.
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