CN106281935A - A kind of method promoting neutral protease activity and the application in beer clarification thereof - Google Patents
A kind of method promoting neutral protease activity and the application in beer clarification thereof Download PDFInfo
- Publication number
- CN106281935A CN106281935A CN201610638191.3A CN201610638191A CN106281935A CN 106281935 A CN106281935 A CN 106281935A CN 201610638191 A CN201610638191 A CN 201610638191A CN 106281935 A CN106281935 A CN 106281935A
- Authority
- CN
- China
- Prior art keywords
- neutral protease
- beer
- clarification
- accelerator
- medicated beer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
- C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
- C12H1/14—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation with non-precipitating compounds, e.g. sulfiting; Sequestration, e.g. with chelate-producing compounds
Abstract
The present invention relates to food processing field, be specifically related to a kind of method promoting neutral protease activity and the application in beer clarification thereof.Promotion neutral protease of the present invention activity and the method for clarifying beer thereof, it is by enzyme accelerator 8 ((4 (2,4 Dimethoxyphenyls) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone add in plasmogen beer together with neutral protease, can prepare the medicated beer of clarification.The neutral protease accelerator of the present invention improves the activity of neutral protease, improves clarifying effect, reduces the usage amount of neutral protease；Present invention can ensure that medicated beer high clarity in transport, cold storage procedure, also can improve the mouthfeel of medicated beer, improve the content of polypeptide in medicated beer, add the nutritive value of medicated beer.
Description
Technical field
The present invention relates to food processing field, be specifically related to a kind of method promoting neutral protease activity and at medicated beer
Application in clarification ".
Technical background
Neutral protease another name serrapeptass, molecular weight is 35～40KD, is that a kind of activity in neutral conditions is higher
Protease, can be used for various protein digestion, and as a kind of biocatalyzer, it is fast that it has catalytic, dirty without industry
The advantages such as dye.According to the regulation of " GB 2760-2011 national food safety standard food additive uses standard ", neutral egg
White enzyme can be used as the processing aid (enzyme preparation) of food industry ".
Medicated beer is in transport, in cold storage procedure, due to the effect of the environmental conditions such as illumination, vibrations, temperature, can produce muddiness,
The phenomenon of precipitation, causing medicated beer reason muddy, precipitation is that in medicated beer, the protein of residual and polyhydric phenols are combined into macromole
Complex.Use neutral protein ferment treatment plasmogen beer, the macro-molecular protein in medicated beer can be decomposed, this method has quickly,
The feature such as easy, effective, has important using value aborning.But neutral protease price is higher, have impact on medicated beer
Production cost and the application of neutral protease ".
8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl)
4H .alpha.-5:6-benzopyran 4 ketone is a kind of dyewood that genistein obtains through chemosynthesis with 1-(2,4-3,5-dimethylphenyl) piperazine
Element derivant.8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl)
4H .alpha.-5:6-benzopyran 4 ketone molecular formula is C28H28N2O5, molecular weight 472.52, its structural formula is as follows ".
At present, 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 are had no both at home and abroad
Hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone application report in terms of promoting neutral protease activity ".
Summary of the invention
It is an object of the invention to provide a kind of employing 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,
7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, as enzyme accelerator, promote the side of neutral protease activity
Method, not only improves the clarifying effect of medicated beer, and the clarifying beer obtained, nutritive value is higher ".
The method of promotion neutral protease of the present invention activity, is by enzyme accelerator 8 ((4 (2,4 dimethoxys
Phenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone and neutral proteins
Enzyme adds in plasmogen beer together, the medicated beer of preparation clarification ".
Wherein:
The addition of neutral protease accelerator is the 2～4% of neutral protease quality, and the addition of neutral protease is every liter
Plasmogen beer adds 4～5mg ".
Hydrolysis temperature is 35～40 DEG C, and enzymolysis time is 32～34h ".
After enzymolysis, use and block the hollow fiber ultrafiltration membrane ultrafiltration that molecular weight is 20000 ".
Neutral protease accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy
3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, for the chemosynthesis of genistein Yu 1-(2,4-3,5-dimethylphenyl) piperazine
Thing, concrete preparation process is as follows ".
Addition genistein 1mmol in 50mL round-bottomed flask, dehydrated alcohol 25mL and 37% formalin 0.08mL,
It is heated to 55～60 DEG C, after reactant liquor clarification, adds 1-(2,4-3,5-dimethylphenyl) piperazine 0.19mL, 48h is stirred at room temperature, has
Solid separates out, and filters, and solid is isolated and purified through recrystallization, obtains the enzyme accelerator of the present invention ".
Neutral protease accelerator be combined with each other with a hydrogen bond with a tyrosine residue of neutral protease, two π
Key and a lysine residue combine, and three π keys and a trp residue combine, and this combination result in neutral protease structure
The change of elephant, thus improve the activity of neutral protease ".
Compared with prior art, beneficial effects of the present invention is as follows ".
(1) the neutral protease accelerator of the present invention improves the activity of neutral protease, and it is together with neutral protease
Add in plasmogen beer, there is synergism, reduce the usage amount of neutral protease, improve the clarifying effect of medicated beer,
Ensure that medicated beer high clarity in transport, cold storage procedure ".
(2) manufacture method of the present invention, improves the mouthfeel of medicated beer, improves the content of polypeptide, adds the battalion of medicated beer
Support and be worth ".
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further ".
Embodiment 1
In plasmogen beer, add the neutral protease of 5mg/L, be simultaneously introduced the neutral protease accounting for neutral protease quality 2%
Accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl)
4H .alpha.-5:6-benzopyran 4 ketone, gas bath concussion 34h under the conditions of temperature is 35 DEG C, after enzymolysis clarification terminates, hollow fiber ultrafiltration membrane
Ultrafiltration, obtains the medicated beer of clarification ".
After adding neutral protease accelerator, ratio does not uses the medicated beer ratio of neutral protease, and clarity improves 8%, and ratio does not makes
Compare clarity with neutral protease accelerator and improve 5%.Use and do not use the medicated beer ratio of neutral protease accelerator not make
4.5% and 1.1% is increased respectively " with the content of peptides of the medicated beer of neutral protease.
Embodiment 2
In plasmogen beer, add the neutral protease of 4mg/L, be simultaneously introduced the neutral protease accounting for neutral protease quality 4%
Accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl)
4H .alpha.-5:6-benzopyran 4 ketone, gas bath concussion 32h under the conditions of temperature is 40 DEG C, after enzymolysis clarification terminates, hollow fiber ultrafiltration membrane
Ultrafiltration, obtains the medicated beer of clarification ".
After adding neutral protease accelerator, ratio does not uses the medicated beer ratio of neutral protease, and clarity improves 11%, and ratio does not uses
Neutral protease accelerator is compared clarity and is improve 8%.Use and do not use the medicated beer ratio of neutral protease accelerator not use
The content of peptides of the medicated beer of neutral protease increases 4.5% and 1.1% respectively ".
Claims (5)
1. one kind promotes the method for neutral protease activity and the application in beer clarification thereof, it is characterised in that: neutral protein
Enzyme accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl)
4H .alpha.-5:6-benzopyran 4 ketone adds in plasmogen beer together with neutral protease, the medicated beer of preparation clarification.
A kind of method promoting neutral protease activity the most according to claim 1 and the application in beer clarification thereof,
It is characterized in that: the addition of enzyme accelerator is the 2～4% of neutral protease quality, the addition of neutral protease be every liter former
Slurry medicated beer adds 4～5mg neutral protease.
A kind of method promoting neutral protease activity the most according to claim 1 and the application in beer clarification thereof,
It is characterized in that: hydrolysis temperature is 35～40 DEG C, enzymolysis time is 32～34 h.
A kind of method promoting neutral protease activity the most according to claim 1 and the application in beer clarification thereof,
It is characterized in that: in plasmogen beer, add neutral protease and neutral protease accelerator, after enzymolysis, ultrafiltration
Medicated beer to clarification；Than not using the medicated beer ratio of neutral protease after adding neutral protease accelerator, clarity improve 8～
11%, improve 5～8% than not using neutral protease accelerator to compare clarity；Use and do not use neutral protease to promote
The medicated beer of agent improves content of peptides than the clarity of the medicated beer not using neutral protease and increases 4.5～8.3% He respectively
1.1～3.2%.
A kind of method promoting neutral protease activity the most according to claim 4 and the application in beer clarification thereof,
It is characterized in that: use and block the hollow fiber ultrafiltration membrane ultrafiltration that molecular weight is 20000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610638191.3A CN106281935A (en) | 2016-08-08 | 2016-08-08 | A kind of method promoting neutral protease activity and the application in beer clarification thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610638191.3A CN106281935A (en) | 2016-08-08 | 2016-08-08 | A kind of method promoting neutral protease activity and the application in beer clarification thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106281935A true CN106281935A (en) | 2017-01-04 |
Family
ID=57666257
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610638191.3A Pending CN106281935A (en) | 2016-08-08 | 2016-08-08 | A kind of method promoting neutral protease activity and the application in beer clarification thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106281935A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603318A (en) * | 2004-07-05 | 2005-04-06 | 南京大学 | Genistein derivatives and their preparation process and use |
| CN102844313A (en) * | 2010-01-28 | 2012-12-26 | 哈佛大学校长及研究员协会 | Compositions and methods for enhancing proteasome activity |
| CN104263712A (en) * | 2014-09-24 | 2015-01-07 | 广西宏美农业科技有限公司 | Composite papain and application thereof |
| CN104311519A (en) * | 2014-09-23 | 2015-01-28 | 山东理工大学 | Preparation and application of functional food factor with gastric mucosal lesion protection effect |
-
2016
- 2016-08-08 CN CN201610638191.3A patent/CN106281935A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603318A (en) * | 2004-07-05 | 2005-04-06 | 南京大学 | Genistein derivatives and their preparation process and use |
| CN102844313A (en) * | 2010-01-28 | 2012-12-26 | 哈佛大学校长及研究员协会 | Compositions and methods for enhancing proteasome activity |
| CN104311519A (en) * | 2014-09-23 | 2015-01-28 | 山东理工大学 | Preparation and application of functional food factor with gastric mucosal lesion protection effect |
| CN104263712A (en) * | 2014-09-24 | 2015-01-07 | 广西宏美农业科技有限公司 | Composite papain and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 耿杰: "槲皮素、染料木素衍生物的合成及其生物活性研究", 《中国优秀硕士学位论文数据库 工程科技I辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TW200744466A (en) | Production process of purified green tea extract | |
| CN103224628B (en) | Hydroxymethylated lignin and application thereof | |
| CN103525778B (en) | Glucose oxidase mutant with high catalytic activity | |
| CN101454438B (en) | Nutritional supplement for alcoholic fermentation media | |
| BR0012712A (en) | Methods of producing l-amino acids by increasing cellular nadph | |
| WO2020140388A1 (en) | Glutamic acid green clean fermentation process | |
| Anasontzis et al. | Effects of temperature and glycerol and methanol‐feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris | |
| MD20070214A (en) | Process for producing wine and wine obtained by said process | |
| TW200744467A (en) | Production process of purified green tea extract | |
| CN106260498A (en) | The manufacture method of a kind of Semen Pisi sativi protein peptide and application | |
| RU2016112467A (en) | METHOD FOR PRODUCING A BEER MASK | |
| CN105380227A (en) | Soy sauce mash preparation process for soy sauce brewage and brewage process for raw soy sauce | |
| GR1007489B (en) | Composition of "radoy" beer and its production method | |
| ES2848049T3 (en) | Procedure for the production of an enzymatic cocktail that uses the liquid residues of a biochemical conversion procedure of lignocellulosic materials | |
| CN107089941A (en) | A kind of method for catalyzing and synthesizing vitamin B6 | |
| CN106281935A (en) | A kind of method promoting neutral protease activity and the application in beer clarification thereof | |
| CN103224454A (en) | Glutamic acid separating and extracting technology | |
| CN106065389A (en) | A kind of method promoting papain activity and the application in beer clarification thereof | |
| CN101864472A (en) | Preparation method of small molecular soybean protein polypeptide liquid nitrogen source for brewing beer | |
| CN108315363B (en) | Citric acid fermentation method for continuously supplementing nitrogen | |
| CN101839912B (en) | Enzymoimmuno screening, fermentation preparation and application of strains used for producing glucosyltransferase | |
| CN111424060B (en) | Biological method for simultaneously preparing D-proline and L-1-pyrroline-5-carboxylic acid | |
| CN102887662A (en) | Cement reinforcing agent and production method thereof | |
| CN106868067A (en) | A kind of temperature sensitive type aminoglutaric acid fermentation production method of utilization mycoprotein hydrolyzate Substitution for Soybean Meal hydrolyzate | |
| CN105942529A (en) | Method for preparing yeast extract with high I+G content |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170104 |
|
| RJ01 | Rejection of invention patent application after publication |