CN106268384A - A kind of automatic dissolving device - Google Patents
A kind of automatic dissolving device Download PDFInfo
- Publication number
- CN106268384A CN106268384A CN201610902580.2A CN201610902580A CN106268384A CN 106268384 A CN106268384 A CN 106268384A CN 201610902580 A CN201610902580 A CN 201610902580A CN 106268384 A CN106268384 A CN 106268384A
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- Prior art keywords
- instrument
- dissolving
- switch
- groove
- reagent
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F21/00—Dissolving
- B01F21/30—Workflow diagrams or layout of plants, e.g. flow charts; Details of workflow diagrams or layout of plants, e.g. controlling means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/80—Mixing plants; Combinations of mixers
- B01F33/85—Mixing plants with mixing receptacles or mixing tools that can be indexed into different working positions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
- B01F35/2201—Control or regulation characterised by the type of control technique used
- B01F35/2209—Controlling the mixing process as a whole, i.e. involving a complete monitoring and controlling of the mixing process during the whole mixing cycle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
- B01F35/221—Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
- B01F35/2211—Amount of delivered fluid during a period
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of automatic dissolving device, including dissolving unit, control unit and operation interface；Wherein, described dissolving unit includes dissolving instrument, reagent bottle and noble gas bottle, and described dissolving instrument is embedded with the time relay, switch and on and off switch；Described dissolving instrument is provided with multiple groove, and the top of described groove is provided with reagent input port；Described dissolving instrument medial surface is provided with precise pressure regulating valve, electromagnetic valve；The side of described dissolving instrument is provided with air pressure instrument, and described air pressure instrument is threadeded with described precise pressure regulating valve；Described control unit controls to be connected with the described time relay, switch, precise pressure regulating valve, electromagnetic valve；Described control unit is connected with described operation interface.The automatic dissolving apparatus structure that the present invention provides is simple and convenient to operate, automaticity is high, it is possible to be accurately controlled the flow of liquid.
Description
Technical field
The present invention relates to DNA detection, dissolving, synthesis device field, particularly relate to a kind of automatic dissolving device.
Background technology (later stage also can supplement herein)
Traditional DNA synthesis material (phosphoramidite monomer) dissolving method mainly uses and carries graduated container to carry out molten
Solving, in course of dissolution, the flow speed of liquid is mainly obtained by the experience of long-term accumulated, leads present in course of dissolution
Seeing many factors, the ratio of precision that result in dissolving is poor, often ingress of air during dissolving, and makes on reagent moisture
Rise, thus can affect the precision of later stage DNA/RNA synthesis.It addition, traditional dissolving method can only carry out single port dissolving, carrying out
When batch dissolves, operation complexity, degree of accuracy is low, waste time and energy.
Summary of the invention
For defect present in prior art, the present invention provides a kind of automatic dissolving device, simple in construction, operation side
Just, automaticity high, the flow of liquid can be accurately controlled.
For above-mentioned technical problem, the technical solution used in the present invention is, the invention provides a kind of automatic dissolving device,
Including dissolving unit, control unit and operation interface；Described dissolving unit includes dissolving instrument, reagent bottle and noble gas bottle, institute
State dissolving instrument and be embedded with the time relay, switch and on and off switch；Described dissolving instrument is provided with groove, and the top of described groove is provided with
Reagent input port；The size of described bottle cap matches with the caliber size of container, it is ensured that in reagent course of dissolution
Not with air contact, maintain original moisture in reagent, avoid the increase of moisture in reagent simultaneously；
Described dissolving instrument medial surface is provided with precise pressure regulating valve, electromagnetic valve；The side of described dissolving instrument is provided with air pressure instrument, institute
State air pressure instrument to threaded with described precise pressure regulating valve；
Described control unit controls to be connected with the described time relay, switch, precise pressure regulating valve, electromagnetic valve；
Described control unit is connected with described operation interface.
Preferably, the quantity value of groove is 1-20, can be equipped with container in each groove, it is possible to realize 1-20 container same
Shi Jinhang dissolves.
Preferably, the described time relay, switch, on and off switch are arranged in order from top to bottom, and described switch includes quantitatively
Volume switch and non-quantitative volume switch, and described determined volume switch is arranged up and down with described non-quantitative volume switch.
Preferably, the side of described dissolving instrument be sequentially provided with from top to bottom the first BULKHEAD UNION, the second BULKHEAD UNION, the 3rd
BULKHEAD UNION, described first BULKHEAD UNION, the second BULKHEAD UNION, the 3rd BULKHEAD UNION 1/4 through dissolve instrument.
Preferably, described first BULKHEAD UNION, the second BULKHEAD UNION are located at the top of described air pressure instrument, and the described 3rd wears
The lower section of described air pressure instrument is located at by plate joint.
Preferably, described dissolving unit also includes reagent bottle and noble gas bottle, one end of described 3rd BULKHEAD UNION with
The gas output tube of described noble gas bottle connects, and the other end is connected with the lower end pipeline of precise pressure regulating valve.
Preferably, one end of described second BULKHEAD UNION is connected with the upper end pipeline of precise pressure regulating valve, the other end and reagent
The gas inlet pipe of bottle connects.
Preferably, one end of described first BULKHEAD UNION is connected with the reagent outlet tube of described reagent bottle, the other end and institute
One end pipeline stating electromagnetic valve connects, and the other end of described electromagnetic valve is by pipeline with the reagent input port on described groove even
Connect.
Preferably, the reagent in described reagent bottle is anhydrous organic solvent, preferably anhydrous acetonitrile.
Preferably, described dissolving instrument is additionally provided with the adjusting pressuring knob of precise pressure regulating valve, temperature sensor and humidity sensor,
Described temperature sensor and humidity sensor are all connected with control unit；Described temperature sensor and humidity sensor are used for detecting
The temperature and humidity of air, it is to avoid the impact on course of dissolution of the temperature and humidity in air；The pressure regulation of described precise pressure regulating valve
Button is connected with described precise pressure regulating valve.
Preferably, the another side of described dissolving instrument is provided with supply socket.
Preferably, described control unit uses any one of PLC control, Control or Single-chip Controlling.
Preferably, described operation interface uses touch LCD screen interface or computer operation interface.
Preferably, the degree of depth value of described groove is 100mm-200mm, and the front portion of described groove is divided into rectangle structure,
The rear portion of described groove is divided into semicircular structure, is more suitable for the placement of container.
Preferably, liquid volume span 5ml-400ml that described dissolving instrument is got.
Use the method that DNA synthesis material (phosphoramidite monomer) is dissolved by above-mentioned automatic dissolving device, including such as
Lower step:
(1) start power supply opening operation interface, control unit, and press the on and off switch dissolved on instrument；
(2) opening noble gas bottle switch, one constant pressure of the pressure regulation button settings of adjustment precision pressure regulator valve is (such as
6PSI)；
(3) opening time relay carries out flux modification by time and volume；
(4) the volume required time (as the volumes of 200 milliliters need the time of 40 seconds) is set
(5) required container being opened lid to be put in groove, the bottleneck of container is relative with the reagent input port of groove top
Should；
(6) press start switch, dissolve instrument automatically beat liquid until set time, dissolve instrument quit work, complete dissolve.
Start button of pressing in above-mentioned (6th) step is the start button on operation interface or dissolves opening on instrument front end face
Close, if determined volume, then press determined volume switch；If non-quantitative volume, then press non-quantitative volume switch, Ke Yishi
The now conversion between determined volume and non-quantitative volume and dissolving.
Compare with prior art, the beneficial effect comprise that:
(1) the automatic dissolving device that the present invention provides, simple in construction, easy to operate, automaticity is high, time saving and energy saving；
(2) the automatic dissolving device that the present invention provides uses the time relay, it is possible to realizes quantitative liquid feeding body, controls accurately
Make the flow of liquid, dissolve control accuracy high；
(3) caliber size of size and the container of the bottle cap on the automatic dissolving device further groove top that the present invention provides
Matching, during dissolving, bottle cap fully seals with vessel port and coordinates, it is to avoid liquid-to-air contacts and increases reagent
The phenomenon of moisture；
(4) dissolver that the present invention provides is possible not only to realize single port dissolving, it is also possible to realizes many mouthfuls of dissolvings, improves
Work efficiency, has saved cost of labor.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the automatic dissolving device that the present invention provides；
Fig. 2 is the structural representation that the single port that the present invention provides dissolves unit；
Fig. 3 is the structural representation () that the single port that the present invention provides dissolves instrument；
Fig. 4 is the internal structure schematic diagram that the single port that the present invention provides dissolves instrument；
Fig. 5 is the structural representation (two) that the single port that the present invention provides dissolves instrument；
Fig. 6-7 is the many mouthfuls of dissolving cell schematics that the present invention provides；
In accompanying drawing, the concrete meaning of each labelling is as follows:
1: dissolve instrument；2: the time relay；3: switch；31: quantitative gas switchs；32: non-quantitative gas switch；4: power supply
Switch；5: reagent input port；6: container；7: the first BULKHEAD UNIONs；8: the second BULKHEAD UNIONs；9: the three BULKHEAD UNIONs；10: air pressure
Instrument；11: precise pressure regulating valve；12: electromagnetic valve；13: relay；14: pipeline；15: adjusting pressuring knob；16: supply socket；17: recessed
Groove；18: reagent bottle；19: noble gas bottle；20: operation interface；21: control unit.
Detailed description of the invention
Below in conjunction with the accompanying drawings, the specific embodiment in the present invention is made detailed elaboration.
Shown in ginseng Fig. 1, the schematic diagram making dissolver by oneself that Fig. 1 provides for the present invention, wherein, automatic dissolving device includes
Dissolve unit, control unit 21 and operation interface 20；The input of control unit 21 is connected with dissolving unit, control unit 21
Outfan is connected with operation interface 20；Control unit 21 uses any one in PLC control, Control or Single-chip Controlling
Control mode；Operation interface 20 uses touch LCD screen interface or computer operation interface；
Shown in ginseng Fig. 2-4, Fig. 2 is the schematic diagram that in the present invention, single port dissolves unit, and wherein, single port dissolves unit and includes molten
Solve instrument 1, reagent bottle 18 and noble gas bottle 19；Fig. 3 is the structural representation dissolving instrument, wherein, dissolves the front end face of instrument 1 from upper
The time relay 2, switch 3 and on and off switch 4 it is embedded with successively under to；Switch is divided into quantitative gas switch 31 and non-quantitative gas to open
Close 32；The front end face of dissolving instrument 1 is additionally provided with and is provided with groove 17, and the top of groove 17 is provided with reagent input port 5；Dissolve the side of instrument
It is sequentially provided with first BULKHEAD UNION the 7, second BULKHEAD UNION 8, air pressure instrument the 10, the 3rd BULKHEAD UNION 9, the first threading from top to bottom
The 1/4 of joint the 7, second BULKHEAD UNION the 8, the 3rd BULKHEAD UNION 9 is through dissolving instrument；The front portion of groove 17 is divided into rectangle structure,
Rear portion is divided into semicircular structure；
Fig. 4 is that in the present invention, single port dissolves instrument internal structure schematic diagram, wherein, dissolve in instrument be provided with precise pressure regulating valve 11,
Electromagnetic valve 12, wherein precise pressure regulating valve 11 is threadeded with air pressure instrument 10；The time relay 2, switch 3, precise pressure regulating valve 11
All control to be connected with control unit with electromagnetic valve 12；
One end of first BULKHEAD UNION 7 is connected with the reagent outlet tube of reagent bottle 18, the other end and one end of electromagnetic valve 12
Pipeline connects, and the other end of electromagnetic valve 12 is connected with the reagent input port 5 on groove by pipeline；
One end of second BULKHEAD UNION 8 is connected with the upper end pipeline of precise pressure regulating valve 11, the other end and the gas of reagent bottle
Input pipe connects；
One end of 3rd BULKHEAD UNION 9 is connected with the gas output tube of noble gas bottle 19, the other end and precise pressure regulating valve
The lower end pipeline of 11 connects；
Shown in ginseng Fig. 5, the back side dissolving instrument is additionally provided with pressure regulation button 15, temperature sensor and the humidity biography that accurate voltage regulating is sent out
Sensor, temperature sensor and humidity sensor are all connected with control unit；The another side dissolving instrument is provided with supply socket 16.
Fig. 6-Fig. 7 is the another kind of embodiment of the present invention, and the most mouthfuls dissolve cell schematics, and many mouthfuls of dissolving unit can be real
Existing multiple containers dissolve simultaneously, improve dissolved efficiency.
When the automatic dissolving device using the present invention to provide carries out DNA/RNA dissolving, first start power supply, opening operation circle
Face, control unit, and press the on and off switch dissolved on instrument, start and dissolve instrument；Then noble gas bottle switch, regulation essence are opened
One constant pressure of the pressure regulation button settings of close pressure regulator valve；It is again turned on the time relay to be carried out with volume by regulating time
Flux modification, sets the volume required time；Being put into needing the container dissolved to open lid in groove, the bottleneck of container is with recessed
The reagent input port at groove top is corresponding；
Press and start switch, dissolve instrument and automatically beat liquid until the time of setting, dissolve instrument and quit work, complete DNA and dissolve.
A series of detailed description mentioned above is only for illustrating of the feasibility embodiment of the present invention,
They are not to limit the scope of the invention, in the ken that described technical field those of ordinary skill is possessed
In, the various changes made on the premise of without departing from present inventive concept belong to protection scope of the present invention.
Claims (9)
1. an automatic dissolving device, including dissolving unit, control unit and operation interface；It is characterized in that, described dissolving is single
Unit includes dissolving instrument, reagent bottle and noble gas bottle, and described dissolving instrument is embedded with the time relay, switch and on and off switch；Described
Dissolving instrument and be provided with multiple groove, the top of described groove is provided with reagent input port；
The medial surface of described dissolving instrument is provided with precise pressure regulating valve, electromagnetic valve；
The side of described dissolving instrument is provided with air pressure instrument, and described air pressure instrument is threadeded with described precise pressure regulating valve；
Described control unit controls to be connected with the described time relay, switch, precise pressure regulating valve, electromagnetic valve；
Described control unit is connected with described operation interface.
2. automatic dissolving device as claimed in claim 1, it is characterised in that the quantity value of described groove is 1-20, each
Groove is built-in with container, it is possible to realizes 1-20 container and dissolves simultaneously.
3. automatic dissolving device as claimed in claim 1, it is characterised in that the described time relay, switch, on and off switch from
Being arranged in order down, described switch includes that determined volume switch and non-quantitative volume switch, described determined volume switch and institute
State non-quantitative volume switch to arrange up and down.
4. automatic dissolving device as claimed in claim 1, it is characterised in that the side of described dissolving instrument sets the most successively
There are the first BULKHEAD UNION, the second BULKHEAD UNION, the 3rd BULKHEAD UNION, described first BULKHEAD UNION, the second BULKHEAD UNION, the 3rd wear
The 1/4 of plate joint is through dissolving instrument；
Described first BULKHEAD UNION, the second BULKHEAD UNION are located at the top of described air pressure instrument, and described 3rd BULKHEAD UNION is located at
The lower section of described air pressure instrument；
Described dissolving unit also includes reagent bottle and noble gas bottle, one end of described 3rd BULKHEAD UNION and described noble gas
The gas output tube of bottle connects, and the other end is connected with the lower end pipeline of precise pressure regulating valve；
One end of described second BULKHEAD UNION is connected with the upper end pipeline of precise pressure regulating valve, and the other end inputs with the gas of reagent bottle
Pipe connects；
One end of described first BULKHEAD UNION is connected with the reagent outlet tube of described reagent bottle, the other end and the one of described electromagnetic valve
End pipeline connects, and the other end of described electromagnetic valve is connected with the reagent input port on described groove by pipeline.
5. automatic dissolving device as claimed in claim 4, it is characterised in that the reagent in described reagent bottle is anhydrous organic molten
Agent.
6. automatic dissolving device as claimed in claim 1, it is characterised in that described dissolving instrument is provided with the pressure regulation of precise pressure regulating valve
Knob, temperature sensor and humidity sensor, described temperature sensor and humidity sensor are all connected with control unit；Described molten
The another side of Xie Yi is provided with supply socket.
7. automatic dissolving device as claimed in claim 1, it is characterised in that described control unit uses PLC to control, relay
Any one of control or Single-chip Controlling；Described operation interface uses touch LCD screen interface or computer operation interface.
8. automatic dissolving device as claimed in claim 1, it is characterised in that the degree of depth value of described groove is 100mm-
200mm, the front portion of described groove is divided into rectangle structure, the rear portion of described groove to be divided into semicircular structure；Described dissolving instrument is beaten
Liquid volume span 5ml-400ml gone out.
9. utilize the automatic dissolving device as described in arbitrary in claim 1-8 to carry out DNA synthesis material (phosphoramidite monomer)
The method dissolved, it is characterised in that comprise the steps:
(1) start power supply opening operation interface, control unit, and press the on and off switch dissolved on instrument；
(2) noble gas bottle switch, one constant pressure of the pressure regulation button settings of adjustment precision pressure regulator valve are opened；
(3) opening time relay carries out flux modification by time and volume；
(4) the volume required time is set；
(5) required container being opened lid to be put in groove, the bottleneck of container is corresponding with the reagent input port of groove top；
(6) starting on push interface switchs, and dissolves instrument and automatically beats liquid until the time of setting, dissolves instrument and quit work,
Complete to dissolve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610902580.2A CN106268384B (en) | 2016-10-17 | 2016-10-17 | A kind of automatic dissolving device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610902580.2A CN106268384B (en) | 2016-10-17 | 2016-10-17 | A kind of automatic dissolving device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106268384A true CN106268384A (en) | 2017-01-04 |
| CN106268384B CN106268384B (en) | 2019-01-22 |
Family
ID=57718347
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610902580.2A Active CN106268384B (en) | 2016-10-17 | 2016-10-17 | A kind of automatic dissolving device |
Country Status (1)
| Country | Link |
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| CN (1) | CN106268384B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111949058A (en) * | 2020-07-29 | 2020-11-17 | 北京擎科生物科技有限公司 | Linkage control system and method for indoor temperature and humidity in DNA synthesizer |
Citations (5)
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| RU2048186C1 (en) * | 1992-01-22 | 1995-11-20 | Александр Александрович Ковалев | Assembly for preparing and metering components |
| CN2748160Y (en) * | 2003-11-18 | 2005-12-28 | 张�林 | Automatic dry medicament powder dissolving system |
| CN201260940Y (en) * | 2008-09-01 | 2009-06-24 | 四川电力试验研究院 | Separated flow type standard humidity generator |
| CN203247079U (en) * | 2013-05-17 | 2013-10-23 | 温州龙华日用电子有限公司 | Pneumatic high-precision automatic quantitative liquid divider |
| CN204121989U (en) * | 2014-10-15 | 2015-01-28 | 江苏天河水务设备有限公司 | Two van-type automatic medicine adding apparatus |
-
2016
- 2016-10-17 CN CN201610902580.2A patent/CN106268384B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2048186C1 (en) * | 1992-01-22 | 1995-11-20 | Александр Александрович Ковалев | Assembly for preparing and metering components |
| CN2748160Y (en) * | 2003-11-18 | 2005-12-28 | 张�林 | Automatic dry medicament powder dissolving system |
| CN201260940Y (en) * | 2008-09-01 | 2009-06-24 | 四川电力试验研究院 | Separated flow type standard humidity generator |
| CN203247079U (en) * | 2013-05-17 | 2013-10-23 | 温州龙华日用电子有限公司 | Pneumatic high-precision automatic quantitative liquid divider |
| CN204121989U (en) * | 2014-10-15 | 2015-01-28 | 江苏天河水务设备有限公司 | Two van-type automatic medicine adding apparatus |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111949058A (en) * | 2020-07-29 | 2020-11-17 | 北京擎科生物科技有限公司 | Linkage control system and method for indoor temperature and humidity in DNA synthesizer |
| CN111949058B (en) * | 2020-07-29 | 2022-02-08 | 北京擎科生物科技有限公司 | Linkage control system and method for indoor temperature and humidity in DNA synthesizer |
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|---|---|
| CN106268384B (en) | 2019-01-22 |
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Effective date of registration: 20190412 Address after: 100176 Beijing Daxing District Beijing Economic and Technological Development Zone 2 Floor 2, No. 25 Courtyard, Jinghai Fourth Road Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 102209 Xisha Industrial Park No. 1, North Qijia Town, Changping District, Beijing Patentee before: BEIJING DNACHEM BIOTECHNOLOGY CO.,LTD. |
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Address after: 100176 Floor 6, West Half Unit, Building 3, Yard 105, Jinghai 3rd Road, Daxing District, Beijing Economic-Technological Development Area Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 100176 Beijing Daxing District Beijing Economic and Technological Development Zone 2 Floor 2, No. 25 Courtyard, Jinghai Fourth Road Patentee before: Beijing Qingke Biotechnology Co.,Ltd. |
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