CN106262870A - A kind of preparation method of Moringa fermentation liquid - Google Patents

A kind of preparation method of Moringa fermentation liquid Download PDF

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Publication number
CN106262870A
CN106262870A CN201610804442.0A CN201610804442A CN106262870A CN 106262870 A CN106262870 A CN 106262870A CN 201610804442 A CN201610804442 A CN 201610804442A CN 106262870 A CN106262870 A CN 106262870A
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moringa
fermentation liquid
preparation
prepare
take
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CN201610804442.0A
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褚晓晨
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Anhui Bio Technology Co Ltd
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Anhui Bio Technology Co Ltd
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Abstract

The invention discloses the preparation method of a kind of Moringa fermentation liquid, including: prepare Moringa enzymolysis solution, prepare fresh pumpkin slurry, ferment and prepare the steps such as Moringa fermentation liquid.The preparation method that the present invention provides, for preparing the Moringa fermentation liquid of clarification, is changed sour taste and the fishy smell of turning sour of tradition Moringa fermentation on local flavor, but is had joyful yoghourt-flavored, and delicate mouthfeel is suitable near water, adds in the various beverages such as taste water, carbonic acid and tea;Although fermentation liquid is clarified, but fermentation liquid has high protein, the unique advantage of high gamma aminobutyric acid content, and protein content reaches more than 0.7wt%, and gamma aminobutyric acid reaches more than 10g/L.Although the Moringa fermentation liquid protein content that the present invention prepares is high, but stable system, can keep 1.5 years occurring without the situation that the systems such as precipitation are unstable in the soda pop that Brix is 8 14%.

Description

A kind of preparation method of Moringa fermentation liquid
Technical field
The invention belongs to food processing field, particularly to the preparation method of a kind of Moringa fermentation liquid.
Background technology
Moringa (drumstick tree) is Moringaceae Moringa perennial plant, originates in the north India Himalayas The quick growing species of trees of southern foot.The root of Moringa, stem, leaf, flower, seed, branch and bark all contain abundant nutritional and officinal compositions, are high Protein, high calcium, high potassium, Gao Mei, high microsteping, homovitamin, low fat, low cholesterol, can increase muscle power, body-building and weight-reducing, fall Blood pressure, control diabetes, overcome insomnia, treatment anemia, suppress pathogenic bacteria high-quality healthy food material, China is at present to Moringa Develop and be still in the starting stage.
Fermentation is a kind of food processing mode gradually accepted by consumers in general, is also tradition food processing mode One of.In numerous fermented foods, the market share that fermentation milk occupies is relatively big, and becomes the trend of steady growth.In recent years, fermentation Breast has newly added various element, if Pan Xinjie etc. is using fresh cow milk, leaf of Moringa powder and white sugar etc. as raw material, develops Moringa milk beverage (Pan Xinjie, Tao Liang, etc. the Technology research [J] of Moringa milk beverage. processing of farm products. 2015 (12)).Pass through the method Although preparing Moringa milk beverage physical and chemical index to meet national standard, but still the acid with Moringa is turned sour taste, and mouthfeel is thicker Rough, and functional single.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that the preparation method of a kind of Moringa fermentation liquid.
For reaching above-mentioned purpose, the preparation method of the Moringa fermentation liquid of present invention design, it is characterised in that include following step Rapid:
(1) prepare Moringa enzymolysis solution: leaf of Moringa is crushed to 100 mesh and obtains leaf of Moringa powder, take 10 parts of leaf of Moringa powder, add 10-100 part Water, after being stirred and heated to 30-50 DEG C of insulation 5min, adds 0.01-0.05 part pectase and 0.02-0.10 part protease, continues Continuous stirring insulation 2-6h, is warming up to 85 DEG C and maintains 10min, and 200 mesh are crossed leaching filtrate and prepared Moringa enzymolysis solution, and cold preservation is standby With;
(2) preparing fresh pumpkin slurry: take fresh pumpkin, add the water of 2 times of Fructus Cucurbitae moschatae quality after peeling, making beating prepares crushed pumpkin, cold Hide standby;
(3) fermentation: take the Moringa enzymolysis solution prepared in 10 parts of steps (1), adds the crushed pumpkin in 0.5-1 part step (2), Add 1.1-1.5 part concentrated whey protein powder, after supertension sterilize, access yeast and lactic acid bacteria, in the 25-35 DEG C of 12-that ferments 36h obtains fermenation raw liquid;
(4) prepare Moringa fermentation liquid: take the fermenation raw liquid that step (3) prepares, sequentially pass through 50 μm sock filtration, dish-style from Scheming is centrifuged, after after 0.22 μm ceramic membrane filter, prepare Moringa fermentation liquid.
Wherein, described concentrated whey protein powder is the concentrated whey protein powder of protein content of whey 30%-40%.
Described yeast is that Kluyveromyces lactis, yeast Kluyveromyces marxianus, schizosaccharomyces pombe or Jie Dingbi are red Yeast.
Described lactic acid bacteria is lactobacillus helveticus, Lactobacillus bulgaricus or lactobacillus thermophilus.
The preparation method that the present invention provides for preparing the Moringa fermentation liquid of clarification, clarified broth be suitable near water, Add in the various beverages such as taste water, carbonic acid and tea.
Although fermentation liquid that the present invention prepares clarification, but fermentation liquid has high protein, high gamma-aminobutyric acid content Unique advantage, protein content reaches more than 0.7wt%, and γ-aminobutyric acid reaches more than 10g/L.
The Moringa fermentation liquid that the preparation method that the present invention provides prepares is changed tradition Moringa fermentation acid on local flavor and is turned sour Taste and fishy smell, but there is joyful yoghourt-flavored, delicate mouthfeel.
Although the Moringa fermentation liquid protein content that the present invention prepares is high, but stable system, it is 8-14%'s for Brix Soda pop can keep 1.5 years occurring without the situation that the systems such as precipitation are unstable.
Detailed description of the invention
Below in conjunction with instantiation, technical scheme is described further.
Embodiment 1:
(1) prepare Moringa enzymolysis solution: leaf of Moringa is crushed to 100 mesh and obtains leaf of Moringa powder, take 10 parts of leaf of Moringa powder, add 10 parts of water, After being stirred and heated to 30 DEG C of insulation 5min, add 0.01 part of pectase and 0.02 part of protease, continue stirring insulation 6h, heat up Maintaining 10min to 85 DEG C, 200 mesh are crossed leaching filtrate and are prepared Moringa enzymolysis solution, and cold preservation is standby;
(2) preparing fresh pumpkin slurry: take fresh pumpkin, add the water of 2 times of Fructus Cucurbitae moschatae quality after peeling, making beating prepares crushed pumpkin, cold Hide standby;
(3) fermentation: take the Moringa enzymolysis solution prepared in 10 parts of steps (1), add the crushed pumpkin in 0.5 part of step (2), then Add the concentrated whey protein powder of 1.1 parts of protein contents 35%, after supertension sterilize, access Kluyveromyces lactis and Switzerland's lactic acid Bacterium, obtains fermenation raw liquid in 25 DEG C of fermentation 36h;
(4) prepare Moringa fermentation liquid: take the fermenation raw liquid that step (3) prepares, sequentially pass through 50 μm sock filtration, dish-style from Scheming is centrifuged, after after 0.22 μm ceramic membrane filter, prepare Moringa fermentation liquid.
The Moringa fermentation milk protein content that this embodiment prepares is 0.75%, and alpha-aminobutyric acid content is 10.8g/L.
Embodiment 2:
(1) prepare Moringa enzymolysis solution: leaf of Moringa is crushed to 100 mesh and obtains leaf of Moringa powder, take 10 parts of leaf of Moringa powder, add 100 parts of water, After being stirred and heated to 50 DEG C of insulation 5min, add 0.05 part of pectase and 0.1 part of protease, continue stirring insulation 2h, heat up Maintaining 10min to 85 DEG C, 200 mesh are crossed leaching filtrate and are prepared Moringa enzymolysis solution, and cold preservation is standby;
(2) preparing fresh pumpkin slurry: take fresh pumpkin, add the water of 2 times of Fructus Cucurbitae moschatae quality after peeling, making beating prepares crushed pumpkin, cold Hide standby;
(3) fermentation: take the Moringa enzymolysis solution prepared in 10 parts of steps (1), add the crushed pumpkin in 1 part of step (2), then add Enter the concentrated whey protein powder of 1.5 parts of protein contents 30%, after supertension sterilize, access yeast Kluyveromyces marxianus and Bulgaria Lactic acid bacteria, obtains fermenation raw liquid in 35 DEG C of fermentation 12h;
(4) prepare Moringa fermentation liquid: take the fermenation raw liquid that step (3) prepares, sequentially pass through 50 μm sock filtration, dish-style from Scheming is centrifuged, after after 0.22 μm ceramic membrane filter, prepare Moringa fermentation liquid.
The Moringa fermentation milk protein content that this embodiment prepares is 1.34%, and alpha-aminobutyric acid content is 12.6g/L.
Embodiment 3:
(1) prepare Moringa enzymolysis solution: leaf of Moringa is crushed to 100 mesh and obtains leaf of Moringa powder, take 10 parts of leaf of Moringa powder, add 80 parts of water, After being stirred and heated to 40 DEG C of insulation 5min, add 0.03 part of pectase and 0.04 part of protease, continue stirring insulation 4h, heat up Maintaining 10min to 85 DEG C, 200 mesh are crossed leaching filtrate and are prepared Moringa enzymolysis solution, and cold preservation is standby;
(2) preparing fresh pumpkin slurry: take fresh pumpkin, add the water of 2 times of Fructus Cucurbitae moschatae quality after peeling, making beating prepares crushed pumpkin, cold Hide standby;
(3) fermentation: take the Moringa enzymolysis solution prepared in 10 parts of steps (1), add the crushed pumpkin in 0.8 part of step (2), then Add the concentrated whey protein powder of 1.3 parts of protein contents 40%, after supertension sterilize, access schizosaccharomyces pombe and thermophilic breast bar Bacterium, obtains fermenation raw liquid in 28 DEG C of fermentation 24h;
(4) prepare Moringa fermentation liquid: take the fermenation raw liquid that step (3) prepares, sequentially pass through 50 μm sock filtration, dish-style from Scheming is centrifuged, after after 0.22 μm ceramic membrane filter, prepare Moringa fermentation liquid.
The Moringa fermentation milk protein content that this embodiment prepares is 1.26%, and alpha-aminobutyric acid content is 14.7g/L.

Claims (4)

1. the preparation method of a Moringa fermentation liquid, it is characterised in that comprise the following steps:
(1) prepare Moringa enzymolysis solution: leaf of Moringa is crushed to 100 mesh and obtains leaf of Moringa powder, take 10 parts of leaf of Moringa powder, add 10-100 part Water, after being stirred and heated to 30-50 DEG C of insulation 5min, adds 0.01-0.05 part pectase and 0.02-0.10 part protease, continues Continuous stirring insulation 2-6h, is warming up to 85 DEG C and maintains 10min, and 200 mesh are crossed leaching filtrate and prepared Moringa enzymolysis solution, and cold preservation is standby With;
(2) preparing fresh pumpkin slurry: take fresh pumpkin, add the water of 2 times of Fructus Cucurbitae moschatae quality after peeling, making beating prepares crushed pumpkin, cold Hide standby;
(3) fermentation: take the Moringa enzymolysis solution prepared in 10 parts of steps (1), adds the crushed pumpkin in 0.5-1 part step (2), Add 1.1-1.5 part concentrated whey protein powder, after supertension sterilize, access yeast and lactic acid bacteria, in the 25-35 DEG C of 12-that ferments 36h obtains fermenation raw liquid;
(4) prepare Moringa fermentation liquid: take the fermenation raw liquid that step (3) prepares, sequentially pass through 50 μm sock filtration, dish-style from Scheming is centrifuged, after after 0.22 μm ceramic membrane filter, prepare Moringa fermentation liquid.
The preparation method of Moringa fermentation liquid the most according to claim 1, it is characterised in that described concentrated whey protein powder is The concentrated whey protein powder of protein content of whey 30%-40%.
The preparation method of Moringa fermentation liquid the most according to claim 1, it is characterised in that described yeast is Kluyveromyces Lactis Dimension yeast, yeast Kluyveromyces marxianus, schizosaccharomyces pombe or outstanding fourth Pichia sp..
The preparation method of Moringa fermentation liquid the most according to claim 1, it is characterised in that described lactic acid bacteria is Switzerland's breast bar Bacterium, Lactobacillus bulgaricus or lactobacillus thermophilus.
CN201610804442.0A 2016-09-06 2016-09-06 A kind of preparation method of Moringa fermentation liquid Pending CN106262870A (en)

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CN107549817A (en) * 2017-08-21 2018-01-09 云南农业大学 A kind of natural organic calcium of Moringa and preparation method thereof
CN109588594A (en) * 2018-12-19 2019-04-09 中国热带农业科学院农产品加工研究所 Probiotic fermentation beverage and preparation method thereof
CN111616356A (en) * 2020-06-05 2020-09-04 龙鼎(内蒙古)农业股份有限公司 Preparation method of moringa oleifera leaf and propolis enzyme and enzyme composition
CN112675084A (en) * 2020-12-23 2021-04-20 昆明颜臻青生物科技有限公司 Moringa oleifera extract for promoting collagen secretion and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107549817A (en) * 2017-08-21 2018-01-09 云南农业大学 A kind of natural organic calcium of Moringa and preparation method thereof
CN107549817B (en) * 2017-08-21 2020-10-30 云南农业大学 Moringa oleifera natural organic calcium and preparation method thereof
CN109588594A (en) * 2018-12-19 2019-04-09 中国热带农业科学院农产品加工研究所 Probiotic fermentation beverage and preparation method thereof
CN111616356A (en) * 2020-06-05 2020-09-04 龙鼎(内蒙古)农业股份有限公司 Preparation method of moringa oleifera leaf and propolis enzyme and enzyme composition
CN112675084A (en) * 2020-12-23 2021-04-20 昆明颜臻青生物科技有限公司 Moringa oleifera extract for promoting collagen secretion and application thereof
CN112675084B (en) * 2020-12-23 2022-11-25 昆明颜臻青生物科技有限公司 Moringa oleifera extract for promoting collagen secretion and application thereof

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Application publication date: 20170104