CN106261464A - A kind of formula expanding nisin bactericidal range and improving sterilizing ability - Google Patents
A kind of formula expanding nisin bactericidal range and improving sterilizing ability Download PDFInfo
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- CN106261464A CN106261464A CN201610680243.3A CN201610680243A CN106261464A CN 106261464 A CN106261464 A CN 106261464A CN 201610680243 A CN201610680243 A CN 201610680243A CN 106261464 A CN106261464 A CN 106261464A
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- China
- Prior art keywords
- solution
- concentration
- acid
- nisin
- sodium
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 65
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 title claims abstract description 54
- 108010053775 Nisin Proteins 0.000 title claims abstract description 54
- 239000004309 nisin Substances 0.000 title claims abstract description 54
- 235000010297 nisin Nutrition 0.000 title claims abstract description 54
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 25
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 129
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 claims abstract description 73
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 claims abstract description 62
- 239000000174 gluconic acid Substances 0.000 claims abstract description 62
- 235000012208 gluconic acid Nutrition 0.000 claims abstract description 62
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 31
- 239000011734 sodium Substances 0.000 claims abstract description 31
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 31
- WDJHALXBUFZDSR-UHFFFAOYSA-N acetoacetic acid Chemical compound CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 claims abstract description 27
- 241000194017 Streptococcus Species 0.000 claims abstract description 25
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000001913 cellulose Substances 0.000 claims abstract description 24
- 229920002678 cellulose Polymers 0.000 claims abstract description 24
- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 24
- 239000004220 glutamic acid Substances 0.000 claims abstract description 24
- IWHVCHNCTHGORM-UHDJGPCESA-M potassium;(e)-3-phenylprop-2-enoate Chemical compound [K+].[O-]C(=O)\C=C\C1=CC=CC=C1 IWHVCHNCTHGORM-UHDJGPCESA-M 0.000 claims abstract description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 22
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 40
- 229960002989 glutamic acid Drugs 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 13
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims description 9
- 229940050410 gluconate Drugs 0.000 claims description 9
- HCFOYBNXMYSROO-UHFFFAOYSA-N [K].C(C=CC1=CC=CC=C1)(=O)O Chemical compound [K].C(C=CC1=CC=CC=C1)(=O)O HCFOYBNXMYSROO-UHFFFAOYSA-N 0.000 claims description 7
- 235000013372 meat Nutrition 0.000 claims description 7
- 239000000176 sodium gluconate Substances 0.000 claims description 6
- 235000012207 sodium gluconate Nutrition 0.000 claims description 6
- 229940005574 sodium gluconate Drugs 0.000 claims description 6
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 229950006191 gluconic acid Drugs 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- RGHNJXZEOKUKBD-KLVWXMOXSA-N L-gluconic acid Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)C(O)=O RGHNJXZEOKUKBD-KLVWXMOXSA-N 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- OCUCCJIRFHNWBP-IYEMJOQQSA-L Copper gluconate Chemical compound [Cu+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O OCUCCJIRFHNWBP-IYEMJOQQSA-L 0.000 claims description 2
- HLCFGWHYROZGBI-JJKGCWMISA-M Potassium gluconate Chemical compound [K+].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O HLCFGWHYROZGBI-JJKGCWMISA-M 0.000 claims description 2
- 229910052797 bismuth Inorganic materials 0.000 claims description 2
- JCXGWMGPZLAOME-UHFFFAOYSA-N bismuth atom Chemical compound [Bi] JCXGWMGPZLAOME-UHFFFAOYSA-N 0.000 claims description 2
- 239000004227 calcium gluconate Substances 0.000 claims description 2
- 235000013927 calcium gluconate Nutrition 0.000 claims description 2
- 229960004494 calcium gluconate Drugs 0.000 claims description 2
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 claims description 2
- 229940108925 copper gluconate Drugs 0.000 claims description 2
- 239000001755 magnesium gluconate Substances 0.000 claims description 2
- 235000015778 magnesium gluconate Nutrition 0.000 claims description 2
- 229960003035 magnesium gluconate Drugs 0.000 claims description 2
- IAKLPCRFBAZVRW-XRDLMGPZSA-L magnesium;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanoate;hydrate Chemical compound O.[Mg+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O IAKLPCRFBAZVRW-XRDLMGPZSA-L 0.000 claims description 2
- 239000004224 potassium gluconate Substances 0.000 claims description 2
- 235000013926 potassium gluconate Nutrition 0.000 claims description 2
- 229960003189 potassium gluconate Drugs 0.000 claims description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 239000004222 ferrous gluconate Substances 0.000 claims 1
- 235000013924 ferrous gluconate Nutrition 0.000 claims 1
- 229960001645 ferrous gluconate Drugs 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 229960001031 glucose Drugs 0.000 claims 1
- 235000001727 glucose Nutrition 0.000 claims 1
- VRIVJOXICYMTAG-IYEMJOQQSA-L iron(ii) gluconate Chemical compound [Fe+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O VRIVJOXICYMTAG-IYEMJOQQSA-L 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229960003975 potassium Drugs 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 13
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- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 241000192125 Firmicutes Species 0.000 abstract description 4
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- 239000003814 drug Substances 0.000 abstract description 3
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 abstract description 2
- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 abstract description 2
- 229930016911 cinnamic acid Natural products 0.000 abstract description 2
- 235000013985 cinnamic acid Nutrition 0.000 abstract description 2
- 235000013373 food additive Nutrition 0.000 abstract description 2
- 239000002778 food additive Substances 0.000 abstract description 2
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 98
- 241000894006 Bacteria Species 0.000 description 40
- 230000000052 comparative effect Effects 0.000 description 22
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- 238000012360 testing method Methods 0.000 description 16
- 241000607142 Salmonella Species 0.000 description 15
- 241000193830 Bacillus <bacterium> Species 0.000 description 14
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- 238000013329 compounding Methods 0.000 description 12
- 239000011670 zinc gluconate Substances 0.000 description 11
- 235000011478 zinc gluconate Nutrition 0.000 description 11
- 229960000306 zinc gluconate Drugs 0.000 description 11
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 10
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 description 10
- 230000000968 intestinal effect Effects 0.000 description 10
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- 230000002147 killing effect Effects 0.000 description 9
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- 150000001875 compounds Chemical class 0.000 description 6
- -1 GLDA Chemical compound 0.000 description 5
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- 235000005979 Citrus limon Nutrition 0.000 description 4
- 244000131522 Citrus pyriformis Species 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- 230000002335 preservative effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241001103617 Pseudomonas aeruginosa ATCC 15442 Species 0.000 description 3
- 241000219095 Vitis Species 0.000 description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 description 3
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- 239000003153 chemical reaction reagent Substances 0.000 description 3
- BEGBSFPALGFMJI-UHFFFAOYSA-N ethene;sodium Chemical group [Na].C=C BEGBSFPALGFMJI-UHFFFAOYSA-N 0.000 description 3
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- 235000010298 natamycin Nutrition 0.000 description 3
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- NAOLWIGVYRIGTP-UHFFFAOYSA-N 1,3,5-trihydroxyanthracene-9,10-dione Chemical compound C1=CC(O)=C2C(=O)C3=CC(O)=CC(O)=C3C(=O)C2=C1 NAOLWIGVYRIGTP-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
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- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000193155 Clostridium botulinum Species 0.000 description 1
- 241000193464 Clostridium sp. Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
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- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 241000231286 Neottia Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 238000005138 cryopreservation Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- IDAGXRIGDWCIET-SDFKWCIISA-L disodium;(2s,3s,4s,5r)-2,3,4,5-tetrahydroxyhexanedioate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O IDAGXRIGDWCIET-SDFKWCIISA-L 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
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- 239000004310 lactic acid Substances 0.000 description 1
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- 235000015097 nutrients Nutrition 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 235000010378 sodium ascorbate Nutrition 0.000 description 1
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 229960005055 sodium ascorbate Drugs 0.000 description 1
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- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000019832 sodium triphosphate Nutrition 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
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- 229940126680 traditional chinese medicines Drugs 0.000 description 1
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- 235000019154 vitamin C Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A kind of formula expanding nisin bactericidal range and improving sterilizing ability, it relates to nisin association area.The invention solves the problems that and be used alone the problem that nisin is only capable of gram positive bacteria plays inhibitory action at present.The solution that the formula of the present invention is made up of streptococcus acidi lactici cellulose solution, glutamic acid diacetic acid four sodium solution, gluconic acid solution, gluconic acid saline solution, potassium cinnamate solution and citric acid solution；The pH=2.5～7.5 of this solution.The present invention has safety, economic, practical feature, it is adaptable to antibacterial, the sterilization operation flow process of the industries such as food, food and drink, daily use chemicals, cultivation.The present invention is widely used in the fields such as food, medicine and daily use chemicals.Cinnamic acid and potassium cinnamate are novel food additive, have good bacteriostasis.
Description
Technical field
The present invention relates to nisin association area.
Background technology
Nisin (English name Nisin) is also known as nisin, is that the one that streptococcus acidi lactici produces is many
Peptide material, is made up of 34 amino acid residues, and molecular weight is about 3500Da.Owing to Nisin can suppress most of Gram-positive
Antibacterial, such as bacillus botulinus, staphylococcus aureus, Streptococcus hemolyticus, Listera, and has the spore of bacillus cereus strongly
Inhibitory action, be therefore widely used in food service industry as food preservative.Generally, sporiferous antibacterial thermostability is very
By force, as sweet milk uses 135 DEG C, instantaneous ultrahigh-temperature sterilization in 2 seconds, the mortality rate of non-spore bacteria is 100%, the death of spore bacteria
Rate 90%, also the spore bacteria of 10% can not be killed.If sweet milk adding 0.03～0.05g/kg Nisin just can suppress bud
Spore bacillus and the germination of Clostridium sp. spores and breeding.Show according to result of study, the antibacterial action of nisin
It is the normal function by interference cell v film, causes the infiltration of cell membrane, nutrient loss and transmembrane potential to decline, thus cause causing
Pathogenic bacteria and the death of putrefaction bacteria cell.
Research shows, nisin and EDTA2Na are used in combination Salmonella, escherichia coli and other gram
Negative bacterium is the most inhibited.The patent of Application No. 200710178631.2 describe a kind of by natamycin and lactic acid
The composite biological preservative prescription of streptostacin composition, possibly together with vitamin C, sodium ethylene diamine tetracetate in this prescription
(EDTA2Na), maltodextrin, sodium tripolyphosphate and sodium hexameta phosphate.Fungus and gram positive bacteria are had by this compound preservative
There is good bacteriostasis.The patent of invention of Patent No. CN 103355730 B discloses a kind of containing nisin
The preparation method of compound preservative, in this patent, the formula of compound preservative is: chitosan 2～6 ‰, AOB 1～
2 ‰, sodium hexameta phosphate 1～5%, natamycin 0.5～1%, garlicin 1～2 ‰, sodium ascorbate 0.05～0.1%, Fructus Hordei Germinatus
Dextrin 1～3% and NaCl 0.5～2%.But the method that this patent is provided has the disadvantages that in actual applications.First,
Product solubility is bad.Chitosan and natamycin are on the premise of pH=6.5, and dissolubility is very poor, according to mention in patent
Addition may not be completely dissolved.Secondly, product stability is relatively low.Garlicin is the product of a kind of oily, dissolve in ethanol,
Ether etc., although but in water, in the water of garlicin, dissolubility is 2.5% (10 DEG C), but have grease precipitate shape after standing
Become.Garlicin is unstable to heat and alkali, and this can be substantially reduced the effectiveness of product.
Nisin has boundless market and application prospect at present, and whole world production capacity is on 1000 tons of left sides at present
Right.But owing to nisin can only suppress most gram positive bacteria, and can not suppress gram negative bacteria and
Fungus, this point is the defect that nisin is natural, also limit the scope of restraining fungi of nisin.There is research table
Bright, nisin and sodium ethylene diamine tetracetate (EDTA2Na) are 10 with the use of can effectively suppress the order of magnitude4～106
Salmonella, the gram negative bacteria such as escherichia coli.But this prescription is detected in research report, and does not prove in reality
In the production application of border effectively.And nisin with sodium ethylene diamine tetracetate antibacterial to gram negative bacteria in theory
Ability is the most extremely limited.
Summary of the invention
The invention aims to solve to be used alone nisin at present be only capable of gram positive bacteria is played
The problem of inhibitory action, the present invention makes compounding prescription also have good to gram negative bacteria and fungus by other compositions compounding
Good inhibitory action.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability of the present invention, it by concentration is
The streptococcus acidi lactici cellulose solution of 0.1～1.0g/L, concentration be 5.0～50.0g/L glutamic acid diacetic acid four sodium solution, concentration be
The gluconic acid solution of 0.5～5.0g/L, concentration be 5.0～50.0g/L gluconic acid saline solution, concentration be 0.1～5.0g/
The solution that the potassium cinnamate solution of L and the citric acid solution that concentration is 1.0～10.0g/L are made；The pH=2.5 of this solution～
7.5。
The present invention comprises following beneficial effect:
1, nisin is (excellent with chelating agen glutamic acid diacetic acid four sodium (GLDA), gluconic acid and gluconate
Select zinc gluconate) gram negative bacteria can be produced good inhibiting effect, and inhibition is better than nisin
Individually compound with EDTA2Na.
2, nisin, GLDA, gluconic acid and gluconate, carries out compounding not by continuation and potassium cinnamate
It is only capable of and further enhances the rejection ability to gram negative bacteria, fungus is also had good rejection ability simultaneously.
The present invention has safety, economic, practical feature, it is adaptable to the industries such as food, food and drink, daily use chemicals, cultivation antibacterial,
Sterilization operation flow process.Glutamic acid diacetic acid four sodium (GLDA) is made up of glutamic acid, and glutamic acid is made after being fermented by plant material,
Therefore have that natural environmental-protective, degradability be good, safety advantages of higher, in European Union can be used for food and drink processing.GLDA
Being the novel chelating agents being just developed recent years, its chelate effect is better than EDTA and the sodium citrate commonly used, with some
The effect of Synergistic can be played during antimicrobial component compound use.Gluconic acid and gluconate are that conventional food adds
Agent or supplementary, be widely used in the fields such as food, medicine and daily use chemicals.Cinnamic acid and potassium cinnamate are novel food
Additive, has good bacteriostasis.
Detailed description of the invention
Detailed description of the invention one: a kind of of present embodiment expands nisin bactericidal range and improve sterilizing ability
Formula, it is by the glutamic acid diethyl that the streptococcus acidi lactici cellulose solution that concentration is 0.1～1.0g/L, concentration are 5.0～50.0g/L
Acid four sodium solutions, concentration be 0.5～5.0g/L gluconic acid solution, concentration be 5.0～50.0g/L gluconate molten
Liquid, concentration are potassium cinnamate solution and the solution made of citric acid solution that concentration is 1.0～10.0g/L of 0.1～5.0g/L；
The pH=2.5～7.5 of this solution.
Detailed description of the invention two: present embodiment is unlike detailed description of the invention one: it by concentration be 0.5～
The streptococcus acidi lactici cellulose solution of 1.0g/L, concentration be 10.0～40.0g/L glutamic acid diacetic acid four sodium solution, concentration be 1.0
～gluconic acid saline solution, the concentration that the gluconic acid solution of 5.0g/L, concentration are 10.0～40.0g/L is 1.0～5.0g/L
Potassium cinnamate solution and the solution made of citric acid solution that concentration is 2.0～8.0g/L；The pH=2.5～7.5 of this solution.
Other is identical with detailed description of the invention one.
Detailed description of the invention three: present embodiment is unlike detailed description of the invention one: it by concentration be 0.5～
The streptococcus acidi lactici cellulose solution of 0.8g/L, concentration be 20.0～40.0g/L glutamic acid diacetic acid four sodium solution, concentration be 2.0
～gluconic acid saline solution, the concentration that the gluconic acid solution of 5.0g/L, concentration are 20.0～40.0g/L is 2.0～5.0g/L
Potassium cinnamate solution and the solution made of citric acid solution that concentration is 2.0～6.0g/L；The pH=2.5～7.5 of this solution.
Other is identical with detailed description of the invention one.
Detailed description of the invention four: present embodiment is unlike detailed description of the invention one: it by concentration be 0.6～
The streptococcus acidi lactici cellulose solution of 0.8g/L, concentration be 30.0～40.0g/L glutamic acid diacetic acid four sodium solution, concentration be 3.0
～gluconic acid saline solution, the concentration that the gluconic acid solution of 5.0g/L, concentration are 30.0～40.0g/L is 3.0～5.0g/L
Potassium cinnamate solution and the solution made of citric acid solution that concentration is 3.0～6.0g/L；The pH=2.5～7.5 of this solution.
Other is identical with detailed description of the invention one.
Detailed description of the invention five: present embodiment is unlike detailed description of the invention one: it by concentration be 0.1～
The streptococcus acidi lactici cellulose solution of 0.8g/L, concentration be 5.0～40.0g/L glutamic acid diacetic acid four sodium solution, concentration be 1.0～
The meat that gluconic acid saline solution that the gluconic acid solution of 5.0g/L, concentration are 5.0～40.0g/L, concentration are 1.0～5.0g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 1.0～5.0g/L are made；The pH=2.5～7.5 of this solution.Other
Identical with detailed description of the invention one.
Detailed description of the invention six: present embodiment is unlike detailed description of the invention one: it by concentration be 0.1～
The streptococcus acidi lactici cellulose solution of 0.5g/L, concentration be 5.0～30.0g/L glutamic acid diacetic acid four sodium solution, concentration be 1.0～
The meat that gluconic acid saline solution that the gluconic acid solution of 4.0g/L, concentration are 5.0～30.0g/L, concentration are 1.0～3.0g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 2.0～8.0g/L are made；The pH=2.5～7.5 of this solution.Other
Identical with detailed description of the invention one.
Detailed description of the invention seven: present embodiment is unlike detailed description of the invention one: it by concentration be 0.2～
The streptococcus acidi lactici cellulose solution of 0.6g/L, concentration be 5.0～20.0g/L glutamic acid diacetic acid four sodium solution, concentration be 2.0～
The meat that gluconic acid saline solution that the gluconic acid solution of 3.0g/L, concentration are 5.0～20.0g/L, concentration are 2.0～4.0g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 4.0～8.0g/L are made；The pH=2.5～7.5 of this solution.Other
Identical with detailed description of the invention one.
Detailed description of the invention eight: present embodiment is unlike detailed description of the invention one: it by concentration be 0.2～
The streptococcus acidi lactici cellulose solution of 0.4g/L, concentration be 10.0～20.0g/L glutamic acid diacetic acid four sodium solution, concentration be 2.0
～gluconic acid saline solution, the concentration that the gluconic acid solution of 5.0g/L, concentration are 10.0～20.0g/L is 1.0～6.0g/L
Potassium cinnamate solution and the solution made of citric acid solution that concentration is 2.0～8.0g/L；The pH=2.5～7.5 of this solution.
Other is identical with detailed description of the invention one.
Detailed description of the invention nine: present embodiment is unlike detailed description of the invention one: it is 0.5g/L's by concentration
Streptococcus acidi lactici cellulose solution, concentration be glutamic acid diacetic acid four sodium solution of 20.0g/L, concentration be that the gluconic acid of 1.0g/L is molten
Liquid, concentration are that the gluconic acid saline solution of 10.0g/L, concentration are the potassium cinnamate solution of 2.0g/L and concentration is the lemon of 4.0g/L
The solution that lemon acid solution is made；The pH=2.5～7.5 of this solution.Other is identical with detailed description of the invention one.
Detailed description of the invention ten: present embodiment is unlike detailed description of the invention one: it is 0.5g/L's by concentration
Streptococcus acidi lactici cellulose solution, concentration be glutamic acid diacetic acid four sodium solution of 20.0g/L, concentration be that the gluconic acid of 2.0g/L is molten
Liquid, concentration are that the gluconic acid saline solution of 20.0g/L, concentration are the potassium cinnamate solution of 3.0g/L and concentration is the lemon of 4.0g/L
The solution that lemon acid solution is made；The pH=2.5～7.5 of this solution.Other is identical with detailed description of the invention one.
Detailed description of the invention 11: present embodiment is unlike detailed description of the invention one: it is 0.1g/L by concentration
Streptococcus acidi lactici cellulose solution, concentration be glutamic acid diacetic acid four sodium solution of 50.0g/L, concentration be the gluconic acid of 0.5g/L
Solution, concentration are that the gluconic acid saline solution of 50.0g/L, concentration are the potassium cinnamate solution of 0.1g/L and concentration is 1.0g/L's
The solution that citric acid solution is made；The pH=2.5～7.5 of this solution.Other is identical with detailed description of the invention one.
Detailed description of the invention 12: present embodiment is unlike detailed description of the invention one: it is 1.0g/L by concentration
Streptococcus acidi lactici cellulose solution, concentration be glutamic acid diacetic acid four sodium solution of 5.0g/L, concentration be the gluconic acid of 5.0g/L
Solution, concentration are that the gluconic acid saline solution of 5.0g/L, concentration are the potassium cinnamate solution of 0.1g/L and concentration is 1.0g/L's
The solution that citric acid solution is made；The pH=2.5～7.5 of this solution.Other is identical with detailed description of the invention one.
Detailed description of the invention 13: present embodiment is unlike detailed description of the invention one: it is 0.5g/L by concentration
Streptococcus acidi lactici cellulose solution, concentration be glutamic acid diacetic acid four sodium solution of 20.0g/L, concentration be the gluconic acid of 2.0g/L
Solution, concentration are that the gluconic acid saline solution of 20.0g/L, concentration are the potassium cinnamate solution of 1.0g/L and concentration is 3.0g/L's
The solution that citric acid solution is made；The pH=2.5～7.5 of this solution.Other is identical with detailed description of the invention one.
Detailed description of the invention 14: present embodiment is unlike detailed description of the invention one: described gluconate
For sodium gluconate, potassium gluconate, magnesium gluconate, calcium gluconate, zinc gluconate, copper gluconate, gluconic acid
Ferrum or gluconic acid bismuth.Other is identical with detailed description of the invention one.
Present invention is not limited only to the content of the respective embodiments described above, one of them or the group of several detailed description of the invention
Contract sample can also realize the purpose of invention.
By following example checking beneficial effects of the present invention:
Experiment reagent: nisin (Nisin, titer >=1000IU/mL), by the safe and sound limited public affairs of biological engineering share
Department provides；Glutamic acid diacetic acid four sodium (GLDA4Na, content >=47%), is provided by Akzo Nobel N.V.；Gluconic acid,
The reagent such as sodium gluconate, zinc gluconate, potassium cinnamate, citric acid are analytical pure, limited by traditional Chinese medicines group chemical reagent
Company provides.
Test strain: staphylococcus aureus ATCC6538, escherichia coli ATCC8099, Pseudomonas aeruginosa
ATCC15442 and candida albicans ATCC10231 is purchased from American Type Culture preservation center (English abbreviation ATCC)；Salmonella
Bacterium CMCC50335 is purchased from Chinese medicine antibacterial preservation administrative center (English abbreviation CMCC)；Citric acid bacillus CICC20211 is purchased from
Chinese industrial Microbiological Culture Collection administrative center (English abbreviation CICC).
Strain culturing method: staphylococcus aureus ATCC6538, escherichia coli ATCC8099, Salmonella
CMCC50335, citric acid bacillus CICC20211 and Pseudomonas aeruginosa ATCC15442 all cultivate with LB fluid medium；
Candida albicans ATCC10231 YEPD culture medium is cultivated.Concrete training method is to take out from-80 DEG C of cryogenic refrigerators
Cryopreservation tube containing 1mL culture presevation liquid one, in the 250mL triangular flask of the LB fluid medium transferred containing 100mL, 37
Take out after cultivating 24h with the speed oscillation of 150rpm at DEG C.Measure the bacteria concentration of cultured each bacterial strain bacteria suspension in 24h, survey
According to bacteria concentration physiological saline solution, bacteria suspension is adjusted to 10 before Shi5～107The viable bacteria initial concentration of CFU/mL.
Method of testing: accurately draw the bacteria suspension 1mL adjusting bacteria concentration, be centrifuged with 4500rpm rotating speed under the conditions of 4 DEG C
5min, enters the thalline after being centrifuged in supernatant discarded, and the phosphate buffered solution of the pH=7.2 adding 1mL in centrifuge tube
Row is resuspended, then resuspended bacterium solution is added in 9mL sterilization test fluid, and quickly static placement 30min after mixing, then carries out viable bacteria
Residual concentration measures.
Sterilizing rate calculates: sterilizing rate=(1-viable bacteria residual concentration/viable bacteria initial concentration) × 100%.
Comparative example 1:
Sterilization test fluid composition: nisin, 0.5g/L (500IU/mL)；Citric acid, 4.0g/L.
The table 1Nisin test fluid sterilizing rate to different microorganisms
Research in comparative example 1 understand (table 1), Nisin in aqueous citric acid solution to staphylococcus aureus
ATCC6538 has preferable killing action, but killing rate is not 100%, and is about 99.99%.And, only Nisin's
Test bactericidal liquid is to four kinds of gram negative bacteria (escherichia coli ATCC8099, Salmonella CMCC50335, citric acid bacillus
CICC20211 and Pseudomonas aeruginosa ATCC15442) and fungus (candida albicans ATCC10231) the most not there is any suppression
Effect, sterilizing rate is 0%.
Comparative example 2:
Sterilization test fluid: nisin, 0.5g/L (500IU/mL)；Disodiumedetate (EDTA2Na),
20.0g/L；Citric acid, 4.0g/L.
Table 2Nisin Yu EDTA2Na compounds the test fluid sterilizing rate to different microorganisms
After nisin is compounding with EDTA2Na, the Nisin sterilization to staphylococcus aureus can be improved further
Rate, also serves certain killing action simultaneously to gram negative bacteria.
It will be seen that working as initial bacteria concentration is 10 from table 26During CFU/mL, Nisin Yu EDTA2Na is compounding to large intestine bar
The sterilizing rate of bacterium, intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa is respectively 90.8462%, 94.1818%,
93.1429% and 91.3000%；When initial bacteria concentration is 105During CFU/mL, Nisin Yu EDTA2Na compounding to escherichia coli,
The sterilizing rate of intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa is respectively 90.2308%, 92.9090%,
87.8571% and 92.6000%.And for this fungus of candida albicans, in the research of comparative example 1 and comparative example 2 not
It is found to have killing action significantly.
Embodiment 1:
Sterilization test fluid: nisin, 0.5g/L (500IU/mL)；Glutamic acid diacetic acid four sodium (GLDA4Na),
15g/L；Gluconic acid, 1.0g/L；Sodium gluconate, 10g/L；Citric acid, 4.0g/L.
Table 3Nisin Yu GLDA, gluconic acid and the sodium gluconate compound sterilizing test fluid sterilizing rate to different microorganisms
After nisin is compounding with GLDA4Na, gluconic acid and sodium gluconate, it is used alone in comparative example 1
Nisin compares, and has been improved the sterilizing rate of staphylococcus aureus equally, and sterilizing rate reaches 100%.Simultaneously with comparative example 2
The killing action of phase comparison gram negative bacteria there has also been and significantly improves.
It will be seen that working as initial bacteria concentration is 10 from table 36During CFU/mL, Nisin Yu GLDA4Na, gluconic acid and Portugal
The compounding sterilizing rate to escherichia coli, intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa of grape sodium saccharate is respectively
94.6932%, 98.9091%, 95.9286% and 94.6000%, sterilizing rate is respectively increased compared with comparative example 2
3.8461%, 4.7273%, 2.7857% and 3.3000%；When initial bacteria concentration is 105During CFU/mL, the experiment of embodiment 1
The sterilizing rate of escherichia coli, intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa is respectively 98.4769%,
98.1273,98.0000% and 96.9000%, be respectively increased 8.2461% compared with comparative example 2,5.2183%,
10.1429% and 4.3000%.
For this fungus of candida albicans, comparative example also finds no killing action significantly.
Embodiment 2:
Sterilization test fluid: nisin, 0.5g/L (500IU/mL)；Glutamic acid diacetic acid four sodium (GLDA), 10g/
L；Gluconic acid, 1.2g/L；Zinc gluconate, 10g/L；Citric acid, 4.0g/L.
Table 4Nisin Yu GLDA, gluconic acid and the zinc gluconate compound sterilizing test fluid sterilizing rate to different microorganisms
After nisin is compounding with GLDA4Na, gluconic acid and zinc gluconate, it is used alone in comparative example 1
Nisin compares, and has been improved the sterilizing rate of staphylococcus aureus, and sterilizing rate reaches 100%.Simultaneously compared with comparative example 2,
The killing action of gram negative bacteria be there has also been and significantly improves by embodiment 2.
It will be seen that working as initial bacteria concentration is 10 from table 46During CFU/mL, Nisin Yu GLDA4Na, gluconic acid and Portugal
The compounding sterilizing rate to escherichia coli, intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa of grape saccharic acid zinc is respectively
95.9231%, 99.2545%, 96.7143% and 97.8000%, sterilizing rate is respectively increased compared with comparative example 2
5.0769%, 5.0727%, 3.5714% and 6.5000%；When initial bacteria concentration is 105During CFU/mL, the experiment of embodiment 2
The sterilizing rate of escherichia coli, intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa is respectively 98.8769%,
99.1727%, 97.9296% and 97.4000%, be respectively increased 8.6461% compared with comparative example 2,6.2637%,
10.0715% and 4.8000%.And the sterilizing rate result in embodiment 2 is slightly above embodiment 1.
For this fungus of candida albicans, the result of study of comparative example 2 also finds no killing action significantly.
Embodiment 3:
Sterilization test fluid: nisin, 0.5g/L (500IU/mL)；Glutamic acid diacetic acid four sodium (GLDA), 10g/
L；Gluconic acid, 1.0g/L；Zinc gluconate, 10g/L；Potassium cinnamate, 2.0g/L；Citric acid, 3.0g/L.
Table 5Nisin from GLDA, gluconic acid, zinc gluconate and potassium cinnamate compound sterilizing test fluid are to different micro-lifes
The sterilizing rate of thing
After nisin and GLDA4Na, gluconic acid, zinc gluconate and potassium cinnamate are compounding, and in comparative example 1
Being used alone Nisin to compare, be obviously improved the sterilizing rate of staphylococcus aureus, sterilizing rate reaches 100%.Simultaneously with
The killing action of comparative example 2 phase comparison gram negative bacteria and candida albicans there has also been and significantly improves.
It will be seen that working as initial bacteria concentration is 10 from table 56During CFU/mL, Nisin Yu GLDA4Na, gluconic acid, Portugal
Grape saccharic acid zinc and potassium cinnamate are compounding to escherichia coli, intestinal Salmonella, citric acid bacillus and the sterilizing rate of Pseudomonas aeruginosa
Being respectively 99.7524%, 99.9974%, 99.9522% and 99.8788%, sterilizing rate is respectively increased compared with comparative example 2
8.9062%, 5.8156%, 6.8093% and 8.5788%；When initial bacteria concentration is 105During CFU/mL, the experiment of embodiment 3
The sterilizing rate of escherichia coli, intestinal Salmonella, citric acid bacillus and Pseudomonas aeruginosa is respectively 99.9600%,
99.9978%, 99.9927% and 99.9956%, be respectively increased 9.7292% compared with comparative example 2,7.0888%,
12.1356% and 7.3956%.And sterilizing rate result to gram negative bacteria is slightly above embodiment 2 in embodiment 3.
Also showing in table 5, the formula in embodiment 3 also has good bactericidal action, sterilizing rate to candida albicans
Higher than 92%, improve nearly 90% compared with comparative example 2.
Embodiment 4:
Sterilization test fluid: nisin, 0.8g/L (800IU/mL)；Glutamic acid diacetic acid four sodium (GLDA4Na),
25g/L；Gluconic acid, 0.5g/L；Zinc gluconate, 15g/L；Potassium cinnamate, 2.5g/L；Citric acid, 4.0g/L.
Table 6Nisin from GLDA, gluconic acid, zinc gluconate and potassium cinnamate compound sterilizing test fluid are to different micro-lifes
The sterilizing rate of thing
It will be seen that prepare sterilization test fluid with the proportioning in embodiment 4 from table 6, bactericidal effect has obtained further
Lifting.When initial bacteria concentration is 106During CFU/mL, in embodiment 4 to escherichia coli, intestinal Salmonella, citric acid bacillus and
The sterilizing rate of Pseudomonas aeruginosa is respectively 99.9429%, 99.9979%, 99.9976% and 99.9973%, with comparative example 2
Compare sterilizing rate and be respectively increased 9.0967%, 5.8161%, 6.8547% and 8.6973%；When initial bacteria concentration is
105During CFU/mL, the experiment of embodiment 3 is to escherichia coli, intestinal Salmonella, citric acid bacillus and the sterilization of Pseudomonas aeruginosa
Rate is respectively 99.9838%, 99.9985%, 99.9927% and 99.9981%, is respectively increased compared with comparative example 2
9.7530%, 7.0895%, 12.1356% and 7.3981%.And sterilizing rate result to gram negative bacteria in embodiment 4
Slightly above embodiment 3.
Also confirming that in table 6, the sterilizing rate of candida albicans is further enhanced by the formula in embodiment 4.With compare
Example 2 is compared and is improve more than 95.6652%, and improves nearly 6% compared with Example 1.
Claims (10)
1. one kind expands nisin bactericidal range and improves the formula of sterilizing ability, it is characterised in that it by concentration is
The streptococcus acidi lactici cellulose solution of 0.1～1.0g/L, concentration be 5.0～50.0g/L glutamic acid diacetic acid four sodium solution, concentration be
The gluconic acid solution of 0.5～5.0g/L, concentration be 5.0～50.0g/L gluconic acid saline solution, concentration be 0.1～5.0g/
The solution that the potassium cinnamate solution of L and the citric acid solution that concentration is 1.0～10.0g/L are made；The pH=2.5 of this solution～
7.5。
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
It is characterised by that it is by the glutamic acid two that the streptococcus acidi lactici cellulose solution that concentration is 0.5～1.0g/L, concentration are 10.0～40.0g/L
The gluconate that gluconic acid solution that acetic acid four sodium solution, concentration are 1.0～5.0g/L, concentration are 10.0～40.0g/L
Solution, concentration be 1.0～5.0g/L potassium cinnamate solution and citric acid solution that concentration is 2.0～8.0g/L make molten
Liquid；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 2, its
It is characterised by that it is by the glutamic acid two that the streptococcus acidi lactici cellulose solution that concentration is 0.5～0.8g/L, concentration are 20.0～40.0g/L
The gluconate that gluconic acid solution that acetic acid four sodium solution, concentration are 2.0～5.0g/L, concentration are 20.0～40.0g/L
Solution, concentration be 2.0～5.0g/L potassium cinnamate solution and citric acid solution that concentration is 2.0～6.0g/L make molten
Liquid；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 3, its
It is characterised by that it is by the glutamic acid two that the streptococcus acidi lactici cellulose solution that concentration is 0.6～0.8g/L, concentration are 30.0～40.0g/L
The gluconate that gluconic acid solution that acetic acid four sodium solution, concentration are 3.0～5.0g/L, concentration are 30.0～40.0g/L
Solution, concentration be 3.0～5.0g/L potassium cinnamate solution and citric acid solution that concentration is 3.0～6.0g/L make molten
Liquid；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
It is characterised by that it is molten by glutamic acid diacetic acid four sodium that the streptococcus acidi lactici cellulose solution that concentration is 0.5g/L, concentration are 20.0g/L
Liquid, concentration be the gluconic acid solution of 1.0g/L, concentration be the gluconic acid saline solution of 10.0g/L, concentration be the meat of 2.0g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 4.0g/L are made；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
It is characterised by that it is molten by glutamic acid diacetic acid four sodium that the streptococcus acidi lactici cellulose solution that concentration is 0.5g/L, concentration are 20.0g/L
Liquid, concentration be the gluconic acid solution of 2.0g/L, concentration be the gluconic acid saline solution of 20.0g/L, concentration be the meat of 3.0g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 4.0g/L are made；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
It is characterised by that it is molten by glutamic acid diacetic acid four sodium that the streptococcus acidi lactici cellulose solution that concentration is 0.1g/L, concentration are 50.0g/L
Liquid, concentration be the gluconic acid solution of 0.5g/L, concentration be the gluconic acid saline solution of 50.0g/L, concentration be the meat of 0.1g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 1.0g/L are made；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
It is characterised by that it is molten by glutamic acid diacetic acid four sodium that the streptococcus acidi lactici cellulose solution that concentration is 1.0g/L, concentration are 5.0g/L
Liquid, concentration be the gluconic acid solution of 5.0g/L, concentration be the gluconic acid saline solution of 5.0g/L, concentration be the Cortex Cinnamomi of 0.1g/L
The solution that acid potassium solution and the citric acid solution that concentration is 1.0g/L are made；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
It is characterised by that it is molten by glutamic acid diacetic acid four sodium that the streptococcus acidi lactici cellulose solution that concentration is 0.5g/L, concentration are 20.0g/L
Liquid, concentration be the gluconic acid solution of 2.0g/L, concentration be the gluconic acid saline solution of 20.0g/L, concentration be the meat of 1.0g/L
The solution that cinnamic acid potassium solution and the citric acid solution that concentration is 3.0g/L are made；The pH=2.5～7.5 of this solution.
A kind of formula expanding nisin bactericidal range and improving sterilizing ability the most according to claim 1, its
The gluconate being characterised by described is sodium gluconate, potassium gluconate, magnesium gluconate, calcium gluconate, glucose
Acid zinc, copper gluconate, ferrous gluconate or gluconic acid bismuth.
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