CN106258957A - A kind of Herba Anoectochili roxburghii is without hormone containerization cultural method and culture medium thereof - Google Patents
A kind of Herba Anoectochili roxburghii is without hormone containerization cultural method and culture medium thereof Download PDFInfo
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- CN106258957A CN106258957A CN201610641550.0A CN201610641550A CN106258957A CN 106258957 A CN106258957 A CN 106258957A CN 201610641550 A CN201610641550 A CN 201610641550A CN 106258957 A CN106258957 A CN 106258957A
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- herba anoectochili
- anoectochili roxburghii
- culture medium
- containerization
- hormone
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention relates to a kind of Herba Anoectochili roxburghii without hormone containerization cultural method and culture medium thereof, solve the technical problem that in prior art, Herba Anoectochili roxburghii is cultivated without hormone containerization, incubation step: (1), with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, contains 200mL culture medium in culture bottle；(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is 25 ± 2 DEG C, and intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days.Cultivating with indoor containerization and replace field cultivation link, after cultivating 90 days, its average plant height reaches 16.5cm.
Description
Technical field
The present invention relates to a kind of Herba Anoectochili roxburghii without hormone containerization cultural method and culture medium thereof, belong to the organic nothing of interpolation
Hormone MS culture medium culturing technical field.
Background technology
Herba Anoectochili roxburghiiAnoectochilus roxburghii(Wal1.) Lindl is that the orchid family Anoectochilus Blume perennial herb is planted
Thing.Herba Anoectochili roxburghii be mainly distributed on the height above sea level such as China Fujian, Taiwan, Guangdong, Guangxi, Hainan, Sichuan, Guizhou and Yunnan 300～
The hilly country of 1200m.In Herba Anoectochili roxburghii, amino acid content, activity of fighting against senium material and micronutrient levels are above wild the West
Ginseng, is a kind of Chinese herbal medicine being widely used.Herb can be used as medicine, and has effect of anti-inflammatory analgetic, nourishing YIN to lower pathogenic fire, heat-clearing and toxic substances removing,
It is mainly used in recent years treating the diseases such as hypertension, diabetes, tumor, rheumatism, dysplasia of children, at Chinese medicine circle, Herba Anoectochili roxburghii
There are " gold grass ", the title of " king of medicine ".Herba Anoectochili roxburghii is in addition to medicinal, and its plant type is small and exquisite attractive in appearance, and vein is golden yellow in meshy arrangement, is also one
Plant the indoor appreciation treasure of great ornamental value.
The seed development of Herba Anoectochili roxburghii is not complete, and germination percentage is extremely low under field conditions (factors), and at present, tissue-culturing rapid propagation has become Herba Anoectochili roxburghii
The Main Means of sapling multiplication.Currently, Herba Anoectochili roxburghii is all by seed or to take stem section etc. and carry out tissue culture's foundation for outer implant
The aseptic rapid propagation system of Herba Anoectochili roxburghii, booth acclimatization and transplants, then gather in the crops seedling.But find in cultivation and production, Herba Anoectochili roxburghii growth ten
Divide slowly, just bind to about 15cm, and whole growth cycle reaches 3-5 month.
If by culture in glassware in the case of without hormone, can directly gathering without field cultivation, also can reach
The even more than plant height of field cultivation and yield, and quality is good, also can save seedling exercising, transplant, the production link such as management,
Growth cycle shortens, cost reduces, and economic benefit improves.Herba Anoectochili roxburghii, without hormone tissue culture, belongs to a kind of height that Herba Anoectochili roxburghii produces
The new way of effect low cost, is the important technical promoting Herba Anoectochili roxburghii fast culture.
Summary of the invention
The present invention provides a kind of Herba Anoectochili roxburghii without hormone containerization cultural method and culture medium thereof, cultivates generation with indoor containerization
For field cultivation link, after cultivating 90 days, its average plant height reaches 16.5cm.
In order to realize the purpose of the present invention, technical scheme specifically includes following steps:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium；
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days；
Described 200mL culture medium includes basal medium and growth medium；
Described basal medium includes MS culture medium and peptone；
Described growth medium includes murphy juice, bean sprout juice, activated carbon, sucrose and agar strip；
Described 200mL culture medium composition comprises:
The peptone of 3g/L；
The murphy juice of 150g/L；
The bean sprout juice of 200g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture medium.
In the present invention, the specific surface area of described activated carbon is 1000-1600m2/g。
Beneficial effects of the present invention:
1, culture medium based on the MS culture medium+peptone without hormone, adds natural organic matter murphy juice and bean sprout simultaneously
Juice carries out containerization cultivation to Herba Anoectochili roxburghii, and peptone, murphy juice, bean sprout juice are the nutrient substance that Herba Anoectochili roxburghii growth provides abundance
And trace element, it is not necessary to the promotion regulation and control of hormone, the Herba Anoectochili roxburghii product gathered does not contains hormonal components, may be used for infant product
Produce；
2, using specific surface area is 1000-1600m2The activated carbon of/g, has certain adsorption, can adsorb because plant continues
The noxious substance that growth produces, builds certain dark surrounds, beneficially root system development simultaneously；
3, Herba Anoectochili roxburghii containing abundant organic without hormone culture-medium in growth promoter well, its average plant height after cultivating 90 days
Arriving 16.5cm, plant height relatively prior art improves 10%, and plant is neat, healthy and strong, color depth, same amount of group of yield ratio
Seedlings cultivating is at the output increased 15% ~ 20% of field cultivation；
4, the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL is used, it is possible to meeting plant and fully grow, 200mL culture medium is that plant continuous production carries
For sufficient nutrition.
Detailed description of the invention
Hereinafter with reference to example, the present invention is described in further detail, but the invention is not restricted to these instantiations.
Specific embodiment 1:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium；
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 15.9cm；
Described 200mL culture medium composition comprises:
The peptone of 3g/L；
The murphy juice of 100g/L；
The bean sprout juice of 150g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture medium.
Specific embodiment 2:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium；
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 16.2cm；
Described 200mL culture medium composition comprises:
The peptone of 3g/L；
The murphy juice of 130g/L；
The bean sprout juice of 180g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture medium.
Specific embodiment 3:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium；
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 16.5cm；
Described 200mL culture medium composition comprises:
The peptone of 3g/L；
The murphy juice of 150g/L；
The bean sprout juice of 200g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture medium.
Specific embodiment 4:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium；
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days, and Herba Anoectochili roxburghii plant height is
Average 16.3cm；
Described 200mL culture medium composition comprises:
The peptone of 3g/L；
The murphy juice of 180g/L；
The bean sprout juice of 250g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture medium.
Claims (3)
1. Herba Anoectochili roxburghii is without a hormone containerization cultural method, it is characterized in that comprising the following steps:
(1) with the Cymbidium ensifolium (L.) Sw. tissue culture bottle of 800mL as culture bottle, culture bottle is contained 200mL culture medium；
(2) in step (1), the Herba Anoectochili roxburghii group of each culture bottle field planting 15 a height of 3cm ~ 5cm of young plant trains Seedling of taking root, and group training temperature is
25 ± 2 DEG C, intensity of illumination is 2000 Lx, and light application time is 14h/d, can gather in the crops Herba Anoectochili roxburghii after cultivating 90 days.
Herba Anoectochili roxburghii the most according to claim 1 is without hormone containerization culture medium, it is characterised in that: described 200mL culture medium
Including basal medium and growth medium；
Described basal medium includes MS culture medium and peptone；
Described growth medium includes murphy juice, bean sprout juice, activated carbon, sucrose and agar strip；
Described 200mL culture medium composition comprises:
The peptone of 3g/L；
The murphy juice of 150g/L；
The bean sprout juice of 200g/L；
The activated carbon of 0.5g/L；
The sucrose of 30g/L；
The agar strip of 8g/L；
Surplus is MS culture medium.
Herba Anoectochili roxburghii the most according to claim 2 is without hormone containerization culture medium, it is characterised in that: the ratio table of described activated carbon
Area is 1000-1600m2/g。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111543328A (en) * | 2020-06-19 | 2020-08-18 | 福建省中科生物股份有限公司 | Gold thread lotus bud seedling liquid tissue culture apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111543328A (en) * | 2020-06-19 | 2020-08-18 | 福建省中科生物股份有限公司 | Gold thread lotus bud seedling liquid tissue culture apparatus |
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