CN106258573A - Carbon nanomaterial improves the method for lawn composting substrate bacillus cereus quantity - Google Patents
Carbon nanomaterial improves the method for lawn composting substrate bacillus cereus quantity Download PDFInfo
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- CN106258573A CN106258573A CN201610443422.5A CN201610443422A CN106258573A CN 106258573 A CN106258573 A CN 106258573A CN 201610443422 A CN201610443422 A CN 201610443422A CN 106258573 A CN106258573 A CN 106258573A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a kind of method using carbon nanomaterial to improve lawn composting substrate bacillus cereus quantity, mainly regulate and control angle from lawn matrix microorganism, assemble as lawn matrix with consumer garbage compost, to mix four kinds of different carbon nanomaterials wherein as process group, cultivated by lawn plant, after different carbon nanomaterial a period of time is added in research, to improve bacillus cereus quantity in substrate.Carbon nanomaterial therein refers to KMnO4Modified nano carbon black, Graphene, CNT and graphene oxide.After carbon nanomaterial is added to turf establishment system, it is achieved the regulation and control of substrate bacillus cereus quantity, provide technical support for carbon nanomaterial bacillus cereus application in garbage compost lawn matrix.
Description
Technical field
The invention belongs to environmental protection technical field, relate to a kind of employing carbon nanomaterial and improve lawn composting substrate spore
The method of bacillus quantity.
Background technology
Carbon nanomaterial is an at least material less than 100 nm in three dimensional structure.Carbon nanomaterial has size
Little, specific surface area is big, and surface can be high, and the feature that surface atom ratio is big shows at aspects such as mechanics, optics, calorifics, electricity
Excellent properties.There is a lot of micro-interface in nano-particle, various interfacial reactions can be strengthened by it, dirty at heavy metal-polluted soil
Dye and sewage purification aspect play obvious action.
Carbon is the highest at nature content, the most extremely important for Organic substance and life entity.1985, scientist
It is found that by 60 former molecular C of C60.CNT and Graphene etc. are found the most in succession subsequently.2004, Geim etc.
People has successfully prepared single-layer graphene.Found successively in the special nature in optical, electrical, thermal and magnetic along with Graphene afterwards,
Research about material with carbon element enters again a brand-new field.
Along with science and technology, being continuously increased of the fast development of modern industry and population, in the world a lot of countries all in
Reveal the trend that water body, soil and atmospheric pollution are the most serious.Report is pointed out, the river of China 50% and the lake of more than 80%
Pool has had been subjected to pollute, and many lakes of III class water standard China of surface water have not reached.The arable land in the whole nation
In have 80,000,000 hm2More than receive pollution in various degree, and distinct issues are exactly organic pollution and weight
The pollution of metal, this situation the most seriously constrains the enforcement of Sustainable Development Strategy of China, has even threatened compatriots'
Healthy.
Although traditional river system repair technology such as cuts dirt, Sediment Dredging etc. temporarily can play some effects, but all controls
Mark does not effects a permanent cure, and biological restoration limiting factor has a lot, such as ambient temperature and Acidity of Aikalinity etc., and this allows for biological restoration visitor
Significant limitation is there is in sight.As traditional contaminated soil remediation method, soil moved in improve the original method, leaching method waste time and energy, and
Well effect is not had for large-area reparation.Compared with traditional environment restoring method, nano material is used for weight
Metallic prosthetic, the absorbability superpower because of it and the specific surface area of super large, the shortcoming that the most there is not traditional method, also show
High remediation efficiency.Additionally atmospheric pollution aspect situation is also SO that is the severeest, that exceed standard in air2, CO and NOxIn the moment
Threaten our healthy.Nano material has splendid catalytic efficiency, even can be catalyzed the most non-incident reaction
New method and thinking is provided for solving generation atmosphere polluting problem.Therefore, utilize nano material solve water body and soil with
And the pollution problem of air is increasingly worth people to pay close attention to.And the research of carbon nanomaterial is the most promising neck
Territory.
Research in recent years finds that CNT can effective heavy metal ion in adsorbed water body.The experiment table of Li etc.
Bright CNT can lead ion in adsorbed water body, and have the biggest dependency with the pH value of solution.Along with solution ph
Increase, the absorbability of lead ion is increased by CNT the most therewith.Langmuir and Freundlich model can solve
Release this adsorption process.Subsequently, Li etc. are investigated the CNT of the different mode of oxidizing process adsorption effect to cadmium ion
Relatively, finding after oxidation processes, the specific surface area of CNT dramatically increases, and the quantity of surface functional group also has substantially increasing
Many.Specifically, CNT is through KMnO4Adsorption effect after oxidation is apparently higher than H2O2And HNO3Oxidation effectiveness.Separately
Outward, next Li et al. has carried out again the experiment of carbon nanotube adsorption copper ion, have studied the CNT of different shape to lead
The absorption of ion is compared, the CNT Adsorption Kinetics to lead ion and the situation of desorption, and discusses carbon
Nanotube is to lead ion, copper ion and cadmium ion competitive Adsorption situation in the same solution.Research finds, CNT pair
It is Pb to the absorption order of three of the above different ions in solution2+＞ Cu2+＞ Cd2+, wherein to lead ion (Pb2+), copper ion
(Cu2+) absorption can with Langmuir equation explain.
Li Yanhui etc. utilize HNO3The CNT of oxidation processes has been carried out Pb2+Adsorption experiment.Research finds HNO3
Carbon nano tube surface after oxidation processes is long-pending to be significantly increased, and additionally the functional group such as-OH ,-CO ,-COOH has been introduced in carbon and receives
The surface of mitron.This can be remarkably reinforced carbon nanotube adsorption Pb2+Active area and active force, thus improve CNT
To Pb2+Adsorbance.Result shows, adsorbance raises along with the rising of temperature, and this explanation CNT is to Pb2+Absorption
It it is an exothermic process.Regeneration test shows, adsorption effect dramatically increases with the increase of pH value.When pH as little as 2, carbon nanometer
The Pb of absorption on pipe2+Desorption rate is to 85%, thus provides theory depend on for realizing recycling of carbon nanotube adsorption material
According to.
In addition, Graphene and graphene oxide for Organic Pollution and are revealed the environmental pollution caused by oil
Also there is the strongest repair.After Graphene modification to the adsorption effect of pollutant in environment more preferably, and adsorbance increase,
Performance is more stable.Chen et al.]The three-dimensional porous graphene oxide film prepared shows huge in terms of selecting absorption
Big potentiality.The weight of its absorption machine oil can reach 37 times of own wt, and the weight of absorbing organic solvent is then more than self
26 times of weight, its adsorption effect compares flake graphite alkene and foamy graphite alkene is much higher.In addition, this porous oxidation
The character of graphene film is sufficiently stable, recycles after the adsorbent on available hexane removal surface.Higher absorption
Amount and longer recycling number of times make this three-dimensional porous graphene oxide film organics removal and cleaning oil pollution
Aspect shows great application prospect.
Along with the junk produced in China's economic growth and the quickening of urbanization speed, urban life and production process
Present the trend dramatically increased.Meanwhile, the supporting process of house refuse is the most complete, and a large amount of rubbish are exposed to city
In, environment is caused the biggest impact.Composting is urban garbage disposal and resource compares mode easily,
This can not only digest the house refuse in city, alleviates the urban environment pressure that house refuse is brought, and also produces simultaneously
The supply agricultural productions of a large amount of compost fertilizer.
Containing nutrient necessary to a large amount of plant growings in house refuse, therefore consumer garbage compostization is processed also
Compost is used for the effective way that agricultural production is its recycling.
Research finds to add appropriate garbage compost to be effectively increased crop yield in the middle of soil.Because of soil physical chemistry
Matter and agrotype are different, and its amplification there is also significant difference.Zhou Dezhi etc.]After have studied interpolation garbage compost, yellowish soil,
The wheat yield change of plantation in moisture soil and red soil.Result shows, after adding appropriate garbage compost, crop all has volume increase effect
Really, wherein red soil effect is best.He Liyuan etc. study discovery, the yield of Plantula Brassicae chinensis along with garbage compost amount of application raising and
Increase, and difference reaches significant level compared with the control.Additionally, crop yield is not only had an impact by using of compost, to crop
Quality also increase significantly effect.The research of square pavilion et al. finds, is added to by garbage compost in reddish brown loam, and soil is made
In the seed of thing Brassica campestris L, the content of protein significantly raises, and protein contained in soybean kernel substantially increases.
But, people also began to recognize and did not contain only nutrient substance in garbage compost in the last few years, simultaneously possibly together with one
Quantitative heavy metal, if chronic administration can increase the content of heavy metal in soil.Therefore, compost is used for agricultural production may
A series of food-safety problem can be brought.Visible, avoiding food chain, to realize the recycling of garbage compost significant.No
Only such, for preventing compost utilization from causing heavy metal pollution of soil, even bring threat to ecological environment, must while application
The problem that heavy metal is repaired must be considered.
Traditional sod production is typically to utilize high-quality soil.The physical property of soil can be had a huge impact by this,
Fertility is caused to decline.Additionally during sod production, the topsoil soils of 2 more than cm can be pulled away, and fertile topsoil soils enters city
Ecosystem, and after sod production several times, agricultural land soil will become more and more barrenr, so that finally cannot enter again
Row agricultural production, this is waste great to land resources.If turf establishment is using consumer garbage compost as primary structure,
Municipal refuse can not only be digested, additionally it is possible to alleviate turf production and bring the destruction of topsoil soils.
Nanotechnology development in recent years is very rapid, all shows wide application at aspects such as material, information, environment
Prospect.Some research theoretical researches show that nano material can produce Different Effects to biological growth, but application nano material regulates and controls
Microbial technique, also there is no correlation technique report.
Above-mentioned analysis shows, the carbon nanomaterial such as Graphene and CNT environmental area application mainly soil,
Water body and atmospheric pollution reparation aspect, and apply as regulator, especially carbon nanomaterial is at compost lawn base
As bacillus cereus adjusting control agent in matter, research there is no document report at present.
Summary of the invention
Carbon nanomaterial just has the heavy metal in the biggest range of application, adsorbed water body and soil because of its special structure
It it is the important application of one of which.This technology regulates and controls angle from lawn matrix microorganism, assembles as lawn with consumer garbage compost
Substrate, to mix four kinds of different carbon nanomaterials wherein as process group, is cultivated by lawn plant, and different carbon is added in research
After nano material a period of time, to improve bacillus cereus quantity in substrate.By carbon nanomaterial being added to turf establishment body
After system, it is achieved the regulation and control of substrate bacillus cereus quantity, for carbon nanomaterial bacillus cereus application in garbage compost lawn matrix
Technical support is provided.
The invention discloses following content for achieving the above object:
A kind of carbon nanomaterial is used to improve the method for antibacterial culturing quantity in lawn composting substrate, it is characterised in that by following
Step is carried out:
(1) experiment material
Experiment soil takes from Tianjin Normal University's campus 0-20 cm topsoil, and fundamental property is: water content 19.4%, pH
7.27, electrical conductivity 2250 S/cm, phosphorus organic, complete, full nitrogen are respectively 52.3,3.75,2.15 g/kg.
Experiment consumer garbage compost is from Tianjin little Dian destructor plant.PH is 7.49, electrical conductivity 2300 S/cm,
Phosphorus organic, complete, full nitrogen are respectively 132,6.81,25.1 g/kg.Heavy metal Cr, Cu, Pb, Zn, Cd content is respectively 703,
341、217、677、5.01 mg/kg。
Being purchased from Qiu Shi carbon black plant, Tianjin for examination nano carbon black (CB), particle diameter is 20 ~ 70 nm, and specific surface area is 1200 m2/
G, pH value is 7.0, utilizes KMnO before experiment4To its modification.
Graphene (G) microplate is purchased from Nanjing Ji Cang nanosecond science and technology company limited, and structure is black foil shape, microplate size
0.5-20 um, thickness 5-25 nm, specific surface area 40-60 m2/ g, density about 2.25 g/cm3, electrical conductivity 8000-10000 S/
M, phosphorus content > 99.5%.
Graphene oxide (GO) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, average thickness 3.4-7
Nm, lamella diameter 10-50 μm, specific surface area 100-300 m2/ g, purity > 90%.
CNT (CNT) is purchased from Beijing and wins space high-tech new material technology company limited, diameter 20-40 nm, length
10-30 um ,-COOH content 1.43%, purity>90wt%, ashes<8wt%, specific surface area>110 m2/ g, conduction: > 102 s/
cm。
(2) experimental technique:
Measure soil moisture content after fetching earth immediately, be calculated the ratio of soil dry weight and weight in wet base.Proportionally calculate to obtain dry weight
Wet soil weight corresponding to 1500 g.Claim the wet soil of corresponding weight mixes with 30 g garbage composts, stir, loading internal diameter
In the plastic flowerpot of high 20 cm of 15 cm, fill 15 basins altogether.
Experiment sets 5 process, Graphene (G), graphene oxide (GO), CNT (CNT), modified Nano white carbon black altogether
(CB) weigh the ratio of 1% in dry ground to be added separately in mixed-matrix, with the process without carbon nanomaterial for comparison (CK),
Fully mix after adding carbon nanomaterial, every basin sowing tall fescue seed 5g.During cultivation, temperature is 19 ~ 27 DEG C, the wettest
Degree is 60 ~ 72%, and every day keeps the skin wet to soil, makes soil moisture be maintained at the 70% of water capacity.After cultivating 130d, will
In basin, 0-5cm deep soil takes out mixing, crosses 60 mesh sieves, and the preservation of-20 DEG C of refrigerator, for microorganism separation and analysis of accounts.
The present invention further discloses employing carbon nanomaterial and improves antibacterial culturing quantitative approach in the composting substrate of lawn
In the application improving lawn composting substrate bacillus cereus quantitative aspects；Carbon nanomaterial therein refers to KMnO4Modified receives
Rice white carbon black, Graphene, CNT and graphene oxide.The particularly application in terms of changing antibacterial composition, carbon therein is received
Rice material refers to nano carbon black and graphene oxide, and experimental result shows: after adding carbon nanomaterial, the bacterium colony group in substrate
Becoming there occurs significantly change, bacillus cereus proportion therein substantially rises, and becomes dominant strain.
KMnO is utilized before nano carbon black of the present invention (CB) experiment4Its modification is referred to: weigh nano-sized carbon
10 g, in 250 mL conical flasks, add 100 mL0.03 mol L-1KMnO4Solution, after standing 10 min, is put in general-purpose
Boiling reflux 1 h on electric heater.After cooling, repeatedly rinse with deionized water, make solution no longer muddy and pH is stable.It is transferred to burn
Cup, dries to constant weight under the conditions of 110 DEG C.
More detailed description of the present invention is as follows:
1 develops materials and methods
1.1 experiment material
Experiment soil takes from Tianjin Normal University's campus 0-20 cm topsoil, and fundamental property is: water content 19.4%, pH
7.27, electrical conductivity 2250 S/cm, phosphorus organic, complete, full nitrogen are respectively 52.3,3.75,2.15 g/kg.
Experiment consumer garbage compost is from Tianjin little Dian destructor plant.PH is 7.49, electrical conductivity 2300 S/cm,
Phosphorus organic, complete, full nitrogen are respectively 132,6.81,25.1 g/kg.Heavy metal Cr, Cu, Pb, Zn, Cd content is respectively 703,
341、217、677、5.01 mg/kg。
Being purchased from Qiu Shi carbon black plant, Tianjin for examination nano carbon black (CB), particle diameter is 20 ~ 70 nm, and specific surface area is 1200 m2/
G, pH value is 7.0, utilizes KMnO before experiment4To its modification.
Graphene (G) microplate is purchased from Nanjing Ji Cang nanosecond science and technology company limited, and structure is black foil shape, microplate size
0.5-20 um, thickness 5-25 nm, specific surface area 40-60 m2/ g, density about 2.25 g/cm3, electrical conductivity 8000-10000 S/
M, phosphorus content > 99.5%.
Graphene oxide (GO) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, average thickness 3.4-7
Nm, lamella diameter 10-50 μm, specific surface area 100-300 m2/ g, purity > 90%.
CNT (CNT) is purchased from Beijing and wins space high-tech new material technology company limited, diameter 20-40 nm, length
10-30 um ,-COOH content 1.43%, purity>90wt%, ashes<8wt%, specific surface area>110 m2/ g, conduction: > 102 s/
cm。
Technology design
Measure soil moisture content after fetching earth immediately, be calculated the ratio of soil dry weight and weight in wet base.Proportionally calculate to obtain dry weight
Wet soil weight corresponding to 1500 g.Claim the wet soil of corresponding weight mixes with 30 g garbage composts, stir, loading internal diameter
In the plastic flowerpot of high 20 cm of 15 cm, fill 15 basins altogether.
Experiment sets 5 process, Graphene (G), graphene oxide (GO), CNT (CNT), modified Nano white carbon black altogether
(CB) weigh the ratio of 1% in dry ground to be added separately in mixed-matrix, with the process without carbon nanomaterial for comparison (CK),
Each process repeats for three times.Fully mix after adding carbon nanomaterial, every basin sowing tall fescue seed 5g.During cultivation, temperature is
19 ~ 27 DEG C, relative humidity is 60 ~ 72%, and every day keeps the skin wet to soil, makes soil moisture be maintained at water capacity
70%.After cultivating 130d, 0-5cm deep soil in basin is taken out mixing, cross 60 mesh sieves, the preservation of-20 DEG C of refrigerator, divide for microorganism
From with analysis of accounts.
Method of production
1.3.1 bacteria culture media compound method
According to Microbiology Experiment handbook, carry out the preparation of microbiological culture media.Wherein cultivate antibacterial beef extract-peptone to train
Supporting base (LB culture medium), concrete formula is Carnis Bovis seu Bubali cream 5g, peptone 10g, sodium chloride 5g, agar 18g, distilled water
1000mL, utilizes NaOH or HCl to regulate pH to 7.0～7.2.121 DEG C of sterilizings 20 minutes, when room temperature is dried in the air to 50-60 DEG C, will training
Foster base is poured in the culture dish of diameter 9cm, and 50ml about poured into by each culture dish, fully dries in the air cool to solidification, standby.
The determination of antibacterial culturing optimum concentration
For being smoothed out of testing, before formal experiment, need to first carry out preliminary experiment.According to Microbiology Experiment instruct and
In the past cultivation experience primarily determined that optimum concentration that antibacterial culturing observes was 10-3-10-5Between.Take the substrate in 0.5g matched group
Soil is poured in the conical flask equipped with 50ml sterilized water, and sealing is placed on shaking table, vibrates 20 min with 150 rpm.Vibration
10 S are stood after stopping.Draw l mL soil extract move in test tube and add 9 mL sterilized water, diluted 10 times, system
Become 10-3The soil extract of concentration, utilizes the method to be respectively prepared 10 afterwards-4、10-5The soil extract of concentration.Take 200ul
Culture dish, in LB culture medium, is inverted in the constant incubator of 37 degree by soil extract even spread, and lucifuge is cultivated.Even
Continuous observation bacterial growth situation, counts after its suitable size quantity is stable.Counting and shape for the ease of microorganism
The differentiation of state feature, should choose colony counts culture medium between 30-100 and count, preliminary experiment found that 10-4This
One concentration is the optimum concentration that in the composting substrate of lawn, antibacterial culturing is observed.
Bacterial plate count procedure
Use the antibacterial in the dilution plate rubbing method isolation medium identical with preliminary experiment, and its bacterium colony is counted.Because
Have determined that optimum concentration is 10 by preliminary experiment-4, so lawn composting substrate dilution 10000 times can directly be carried out by experiment
Cultivate.Specific operation process is: weighing 0.5g soil and be poured in the conical flask equipped with 50 mL sterilized water, sealing is placed on
On shaking table, vibrate 20 min with 150 rpm.Vibration stands 10 s after stopping.Draw 0.5 mL soil extract and move into dress
Have in the conical flask of 50ml sterilized water and make 10-4After the soil suspension of concentration, fully mixing, draw 200uL and injected
On solid LB media, coating uniformly, is cultivated 3d in culture dish is inverted in 37 DEG C of constant incubators afterwards, is chosen bacterium colony
Number flat board in the range of 30-300 carries out separating counting, and according to its different colony characteristicses classification, numbering.After sterilizing
Inoculating loop picking bacterial clump culture the most of the same race is inoculated in new LB culture medium the most respectively, draws continuous print broken line,
To reach the purpose to the bacteria purification being separated to.Carry out label after inoculation and be inverted in 37 degree of incubators cultivation 2d, turn afterwards
Move on to 4 DEG C of refrigerators save backup.
Bacterial community multiformity method
Use Microsoft Excel 2007 and SPSS 17. 0 software that data carry out statistical analysis, use single factor test side
The difference of difference analysis (one-way ANOVA) inspection various features index, analyzes its difference with Duncan duncan's new multiple range method
Significance (α=0.05).Shannon (Shannon-Wiener index) diversity indices (H), the uniformity (Pielou) is used to refer to
Number (E) and richness (Margalef) index (D) represent Phylogenetic diversity of bacteria, and its formula is:
It is in groupIndividual
The number of individuals of species accounts for the ratio of total individual number in group,The number of individuals of species, H is the kind of actual observation
Multiformity,For maximum specific diversity, S is the species number in group, and N is sum individual in group.
2 development results analyses
Cultivable bacteria sum in 2.1 lawn composting substrates
In different disposal soil, cultivable bacteria total plate count is shown in Fig. 1.Add carbon nanomaterial can significantly reduce in substrate can
Cultivating bacterial number, different carbon nanomaterial effects are different, but difference is the most notable.Carbon nanomaterial processes can be cultivated carefully
Bacterium colony counts compare with comparison be respectively provided with significant difference (P＜ 0.05), wherein comparison clump count is most, has reached 2.8 × 106
cfu·g-1, carbon nanomaterial processes bacteria colony count and is followed successively by Graphene from high to low > and CNT > nanometer carbon black > oxidation stone
Ink alkene processes, and is comparison the 60.12% of clump count, 42.56%, 25.89%, 23.66% respectively.
Cultivable bacteria multiformity in 2.2 lawn composting substrates
Bacterial strain statistical result is carried out community diversity computing, and result is as shown in Figure 2.Interpolation carbon nanomaterial can make can in substrate
Cultivating bacterial community multiformity to reduce, it is more that graphene oxide and nano carbon black process decline, and CNT processes and reduces
Few, compare close with CK diversity index.
The diversity indices of control treatment antibacterial is up to 2.56, and Graphene processes and CNT processes diversity indices
Be respectively 2.25 and 2.41, respectively be comparison 87.79% and 94.08%, with compare between difference the most notable.Graphene oxide and receiving
Rice carbon black processes diversity indices and is respectively 1.86 and 1.71, compares with comparison, reduces 27.48% and 33.15% respectively, poor
Different significantly.
CNT processes does not has difference with CK evenness index, is 1.84.Graphene and graphene oxide process and divide
It not the 78.74% and 69.10% of comparison.Nano carbon black processes minimum, for the 66.78% of matched group, notable with contrast difference.
The diversity index that CK processes and CNT processes is higher, respectively 0.73 and 0.70, and nano carbon black and oxygen
It is relatively low that functionalized graphene processes diversity index, respectively 0.43 and 0.47, is the 59.09% and 63.64% of comparison, has significantly with CK
Difference.Graphene is processed as 0.53, is the 72.72% of comparison, the most notable with matched group difference.
Bacterial clump composition in 2.3 lawn composting substrates
In different disposal substrate, bacterial clump composition and proportion thereof are shown in Table 1.In addition to total number of bacteria there are differences, respectively process
The sociales of middle cultivable bacteria also there occurs change.Find through 16SrDNA order-checking, this technology isolated 10 strains not similar shape
The antibacterial of state adheres to 5 genus separately.It is false xanthomonas that CK processes sociales, and its quantity is most.Carbon nanomaterial processes spore bar
Pseudomonas quantitatively shows significant advantage, becomes sociales.After adding nano material, root nodule bacteria have a declining tendency, and
And no longer there are root nodule bacteria in nano carbon black and graphene oxide process group.This shows that the membership that adds of carbon nano adsorption material affects
The sociales of antibacterial in the composition of substrate antibacterial and then change substrate.
Cultivable bacteria composition and ratio (%) in table 1 different disposal soil
3 develop conclusion
Adding after carbon nanomaterial, the bacterium colony composition in substrate there occurs significantly change, bacillus cereus proportion substantially on
Rise, become dominant strain.
Accompanying drawing illustrates:
Fig. 1 is cultivable bacteria total plate count in different disposal soil (unit: × 105cfu g-1)；Wherein CK represents comparison
Group, G represent that interpolation Graphene, CNT represent that interpolation CNT, CB represent that interpolation nano carbon black, GO represent interpolation graphite oxide
Alkene.In figure, different lowercase alphabets show significant difference (p < 0.05), lower same；
Fig. 2 is cultivable bacteria bacterium colony diversity indices in different disposal soil.
Detailed description of the invention:
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is
Method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and the model of the unrestricted present invention
Enclosing, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of bright spirit and scope, the various changes or the change that carry out the material component in these embodiments and consumption also belong to
In protection scope of the present invention.The present invention is raw materials used, reagent is commercially available.
Embodiment 1
(1) experiment material
Experiment soil takes from Tianjin Normal University campus 10 cm topsoil, and fundamental property is: water content 19.4%, pH
7.27, electrical conductivity 2250 S/cm, phosphorus organic, complete, full nitrogen are respectively 52.3,3.75,2.15 g/kg.
Experiment consumer garbage compost is from Tianjin little Dian destructor plant.PH is 7.49, electrical conductivity 2300 S/cm,
Phosphorus organic, complete, full nitrogen are respectively 132,6.81,25.1 g/kg.Heavy metal Cr, Cu, Pb, Zn, Cd content is respectively 703,
341、217、677、5.01 mg/kg。
Being purchased from Qiu Shi carbon black plant, Tianjin for examination nano carbon black (CB), particle diameter is 20 nm, and specific surface area is 1200 m2/ g,
PH value is 7.0, utilizes KMnO before experiment4To its modification.
Graphene (G) microplate is purchased from Nanjing Ji Cang nanosecond science and technology company limited, and structure is black foil shape, microplate size
0.5 um, thickness 5 nm, specific surface area 40 m2/ g, density about 2.25 g/cm3, electrical conductivity 8000 S/m, phosphorus content > 99.5%.
Graphene oxide (GO) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, average thickness 3.4-7
Nm, lamella diameter 10 μm, specific surface area 100 m2/ g, purity > 90%.
CNT (CNT) is purchased from Beijing and wins space high-tech new material technology company limited, diameter 20 nm, length
10um ,-COOH content 1.43%, purity>90wt%, ashes<8wt%, specific surface area>110 m2/ g, conduction: > 102 s/cm.
(2) experimental technique:
Measure soil moisture content after fetching earth immediately, be calculated the ratio of soil dry weight and weight in wet base.Proportionally calculate to obtain dry weight
Wet soil weight corresponding to 1500 g.Claim the wet soil of corresponding weight mixes with 30 g garbage composts, stir, loading internal diameter
In the plastic flowerpot of high 20 cm of 15 cm, fill 15 basins altogether.
Experiment sets 5 process, Graphene (G), graphene oxide (GO), CNT (CNT), modified Nano white carbon black altogether
(CB) weigh the ratio of 1% in dry ground to be added separately in mixed-matrix, with the process without carbon nanomaterial for comparison (CK),
Fully mix after adding carbon nanomaterial, every basin sowing tall fescue seed 5g.During cultivation, temperature is 19 DEG C, and relative humidity is
60%, every day keeps the skin wet to soil, makes soil moisture be maintained at the 70% of water capacity.After cultivating 130d, by deep for 1cm in basin
Degree soil takes out mixing, crosses 60 mesh sieves, and the preservation of-20 DEG C of refrigerator, for microorganism separation and analysis of accounts.
Embodiment 2
(1) experiment material
Experiment soil takes from Tianjin Normal University campus 20 cm topsoil, and fundamental property is: water content 19.4%, pH
7.27, electrical conductivity 2250 S/cm, phosphorus organic, complete, full nitrogen are respectively 52.3,3.75,2.15 g/kg.
Experiment consumer garbage compost is from Tianjin little Dian destructor plant.PH is 7.49, electrical conductivity 2300 S/cm,
Phosphorus organic, complete, full nitrogen are respectively 132,6.81,25.1 g/kg.Heavy metal Cr, Cu, Pb, Zn, Cd content is respectively 703,
341、217、677、5.01 mg/kg。
Being purchased from Qiu Shi carbon black plant, Tianjin for examination nano carbon black (CB), particle diameter is 70 nm, and specific surface area is 1200 m2/ g,
PH value is 7.0, utilizes KMnO before experiment4To its modification.
Graphene (G) microplate is purchased from Nanjing Ji Cang nanosecond science and technology company limited, and structure is black foil shape, microplate size 20
Um, thickness 25 nm, specific surface area 60 m2/ g, density about 2.25 g/cm3, electrical conductivity 10000 S/m, phosphorus content > 99.5%.
Graphene oxide (GO) is purchased from Suzhou Heng Qiu nanometer company, for black or brownish yellow powder, average thickness 7 nm, sheet
Layer diameter 50 μm, specific surface area 100-300 m2/ g, purity > 90%.
CNT (CNT) is purchased from Beijing and wins space high-tech new material technology company limited, diameter 40 nm, length 30
Um ,-COOH content 1.43%, purity>90wt%, ashes<8wt%, specific surface area>110 m2/ g, conduction: > 102 s/cm.
(2) experimental technique:
Measure soil moisture content after fetching earth immediately, be calculated the ratio of soil dry weight and weight in wet base.Proportionally calculate to obtain dry weight
Wet soil weight corresponding to 1500 g.Claim the wet soil of corresponding weight mixes with 30 g garbage composts, stir, loading internal diameter
In the plastic flowerpot of high 20 cm of 15 cm, fill 15 basins altogether.
Experiment sets 5 process, Graphene (G), graphene oxide (GO), CNT (CNT), modified Nano white carbon black altogether
(CB) weigh the ratio of 1% in dry ground to be added separately in mixed-matrix, with the process without carbon nanomaterial for comparison (CK),
Fully mix after adding carbon nanomaterial, every basin sowing tall fescue seed 5g.During cultivation, temperature is 27 DEG C, and relative humidity is
72%, every day keeps the skin wet to soil, makes soil moisture be maintained at the 70% of water capacity.After cultivating 130d, by deep for 5cm in basin
Degree soil takes out mixing, crosses 60 mesh sieves, and the preservation of-20 DEG C of refrigerator, for microorganism separation and analysis of accounts.
Claims (4)
1. one kind uses carbon nanomaterial to improve the method for antibacterial culturing quantity in the composting substrate of lawn, it is characterised in that by as follows
Step carry out:
(1) experiment material
Experiment soil takes 0-20 cm topsoil；
Being 20 ~ 70 nm for examination nano carbon black particle diameter, specific surface area is 1200 m2/ g, pH value is 7.0, utilizes KMnO before experiment4
To its modification；
Graphene microchip size 0.5-20 um, thickness 5-25 nm, specific surface area 40-60 m2/ g, density about 2.25 g/cm3, electricity
Conductance 8000-10000 S/m, phosphorus content > 99.5%；
Graphene oxide average thickness 3.4-7 nm, lamella diameter 10-50 μm, specific surface area 100-300 m2/ g, purity > 90%；
Carbon nanotube diameter 20-40 nm, length 10-30 um ,-COOH content 1.43%, purity>90wt%, ashes<8wt%, than
Surface area > 110 m2/ g, conduction: > 102 s/cm；
(2) experimental technique:
Measure soil moisture content after fetching earth immediately, be calculated the ratio of soil dry weight and weight in wet base, proportionally calculate to obtain dry weight
Wet soil weight corresponding to 1500 g, claim the wet soil of corresponding weight mixes with 30 g garbage composts, stir, loading internal diameter
In the plastic flowerpot of high 20 cm of 15 cm, fill 15 basins altogether；
Experiment sets 5 process altogether: Graphene (G), graphene oxide (GO), CNT (CNT), modified Nano white carbon black (CB) are pressed
Dry ground weighs the ratio of 1% and is separately added into, and fully mixes, be right with the process without carbon nanomaterial after adding carbon nanomaterial
According to (CK), every basin sowing tall fescue seed 5g, during cultivation, temperature is 19 ~ 27 DEG C, and relative humidity is 60 ~ 72%, and every day is given
Soil keeps the skin wet, and makes soil moisture be maintained at the 70% of water capacity, after cultivating 130d, is taken by 0-5cm deep soil in basin
Going out mixing, cross 60 mesh sieves, the preservation of-20 DEG C of refrigerator, for microorganism separation and analysis of accounts.
2. the method described in claim 1, wherein the fundamental property of soil is: water content 19.4%, pH 7.27, electrical conductivity 2250
S/cm, phosphorus organic, complete, full nitrogen are respectively 52.3,3.75,2.15 g/kg；
Experiment consumer garbage compost is from Tianjin little Dian destructor plant, and pH is 7.49, and electrical conductivity 2300 S/cm is organic
Matter, full phosphorus, full nitrogen are respectively 132,6.81,25.1 g/kg；
Heavy metal Cr, Cu, Pb, Zn, Cd content are respectively 703,341,217,677,5.01 mg/kg；
Nano carbon black, particle diameter is 20 ~ 70 nm, and specific surface area is 1200 m2/ g, pH value is 7.0, utilizes KMnO before experiment4Right
Its modification；
Graphene microchip size 0.5-20 um, thickness 5-25 nm, specific surface area 40-60 m2/ g, density about 2.25 g/cm3, electricity
Conductance 8000-10000 S/m, phosphorus content > 99.5%；
Graphene oxide average thickness 3.4-7 nm, lamella diameter 10-50 μm, specific surface area 100-300 m2/ g, purity > 90%；
CNT, diameter 20-40 nm, length 10-30 um ,-COOH content 1.43%, purity>90wt%, ashes<8wt%, than
Surface area > 110 m2/ g, conduction: > 102 s/cm.
3. during the employing carbon nanomaterial described in claim 1 improves lawn composting substrate, antibacterial culturing quantitative approach is improving grass
The application of level ground composting substrate bacillus cereus quantitative aspects；Carbon nanomaterial therein refers to KMnO4The nano carbon black of modification, stone
Ink alkene, CNT and graphene oxide.
4. during the employing carbon nanomaterial described in claim 1 improves lawn composting substrate, antibacterial culturing quantitative approach is changing carefully
Application in terms of bacterium composition, carbon nanomaterial therein refers to nano carbon black and graphene oxide.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117099633A (en) * | 2023-10-19 | 2023-11-24 | 中国农业科学院都市农业研究所 | Cultivation method of gastrodia elata |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012028964A2 (en) * | 2010-09-03 | 2012-03-08 | Indian Institute Of Technology | Reduced graphene oxide-based-composites for the purification of water |
| CN103155846A (en) * | 2013-03-19 | 2013-06-19 | 江苏省中国科学院植物研究所 | Method of improving germination rates of isatis root seeds using multi-wall carboxylating carbon nano tubes |
| CN103797923A (en) * | 2014-03-11 | 2014-05-21 | 天津师范大学 | Method for improving reproducibility of soil matrix lawn grass through modified nanocarbon |
| CN103804100A (en) * | 2014-03-11 | 2014-05-21 | 天津师范大学 | Method for controlling growth of compost substrate festuca arundinacea by using modified nano carbon |
| CN103819275A (en) * | 2014-03-11 | 2014-05-28 | 天津师范大学 | Method for controlling different forms of heavy metal in urban life compost by modified nano carbon |
-
2016
- 2016-06-21 CN CN201610443422.5A patent/CN106258573A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012028964A2 (en) * | 2010-09-03 | 2012-03-08 | Indian Institute Of Technology | Reduced graphene oxide-based-composites for the purification of water |
| CN103155846A (en) * | 2013-03-19 | 2013-06-19 | 江苏省中国科学院植物研究所 | Method of improving germination rates of isatis root seeds using multi-wall carboxylating carbon nano tubes |
| CN103797923A (en) * | 2014-03-11 | 2014-05-21 | 天津师范大学 | Method for improving reproducibility of soil matrix lawn grass through modified nanocarbon |
| CN103804100A (en) * | 2014-03-11 | 2014-05-21 | 天津师范大学 | Method for controlling growth of compost substrate festuca arundinacea by using modified nano carbon |
| CN103819275A (en) * | 2014-03-11 | 2014-05-28 | 天津师范大学 | Method for controlling different forms of heavy metal in urban life compost by modified nano carbon |
Non-Patent Citations (1)
| Title |
|---|
| 吴春来 樊静: "石墨烯材料在重金属废水吸附净化中的应用", 《化工进展》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117099633A (en) * | 2023-10-19 | 2023-11-24 | 中国农业科学院都市农业研究所 | Cultivation method of gastrodia elata |
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