CN106248942A - A kind of urine Exfoliated tumor cells Dual channel detection equipment - Google Patents
A kind of urine Exfoliated tumor cells Dual channel detection equipment Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57473—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving carcinoembryonic antigen, i.e. CEA
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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Abstract
The invention provides a kind of urine Exfoliated tumor cells Dual channel detection equipment, including control device, and all connect from described control device electric supply installation, driving means, in order to hold the holding apparatus of optical filter and in order to detect the fluorescence detection device of at least 2 different fluorescence signals.This urine Exfoliated tumor cells Dual channel detection equipment, along with the switching of optical filter in holding apparatus, can effectively carry out the recognition detection of two or more different fluorescence signal；Thus realize the specific detection of urine Exfoliated tumor cells on same chip to be checked, it is to avoid because the cell surface protein differential expression of tumor cell and flase drop or the problem of missing inspection, improve the accuracy of detection.Meanwhile, the design of multi-channel detection device structure is succinct, compact, is convenient for carrying, and easy and simple to handle, cost reduces, and is suitable for classification diagnosis and treatment or domestic, can be effectively improved urologic neoplasms examination rate in mid-aged population.
Description
Technical field
The invention belongs to the technical field of detection equipment, in particular to a kind of urine Exfoliated tumor cells dual pathways
Detection equipment.
Background technology
Urinary system kinds of tumor mainly includes renal carcinoma, bladder cancer and carcinoma of prostate three kinds.Renal carcinoma accounts for pernicious swelling of being grown up
The 2%～3% of tumor, accounts for the 80%～90% of Adult kidney malignant tumor.In world wide, the sickness rate of various countries or each department is each
Differing, developed country's sickness rate is higher than developing country generally, and urban area is higher than rural area, and male is more than women,
Men and women's Proportion of patients is about 2: 1, and age of onset is found in each age group, age 50～70 years old occurred frequently.Bladder cancer refers to occur
Malignant tumor in bladder mucosa.It is the modal malignant tumor of urinary system, is also one of big kinds of tumor of whole body ten.Account for me
First of state's Genitourinary system sickness rate, and in west, its sickness rate is only second to carcinoma of prostate, occupies the 2nd.Prostate
Cancer, then for betide the malignant tumor in human male prostate tissue, be the result of prostatic acini cell exception random growth, it
Being the modal malignant tumor of male, in the genitourinary system tumor of west, sickness rate occupies the 1st.Therefore, urinary system is raw
Grow the detection of system tumor cell, contribute to the clinical diagnosis of tumor patient, prognosis evaluation, disease surveillance etc., individuation is controlled
Treatment is extremely important.
Exfoliated tumor cells other cellular identification technology of use in conjunction must determine the kind of cell after capture
And source.Therefore, select specific detection technique or method that tumor cell is carried out confirmation to be whole detection, identified
The committed step of journey.The detection method of conventional urinary system Exfoliated tumor cells has polymerase chain reaction method (PCR), immunity thin
Born of the same parents' chemical technology (Immunocytochemistry, ICC), fluorescence in situ hybridization technique (Fluorescence In Situ
Hybridization, FISH) and flow cytometry (FCM), fiber array scanning (FAST) etc..But prior art is still deposited
: such as, PCR and various improvement technology thereof are to detect tumor cell most efficient method at present, but owing to mensuration process need to be broken
Bad tumor cell, therefore cannot carry out morphological observation to cell.Immunocytochemical technique and fluorescence in situ hybridization technique are just
To carry out in situ detection for TS albumen or gene on the basis of cell enrichment, thus complete after screening thin
The qualification of born of the same parents, but, owing to unstability and other normal cells of tumor cell proteins/gene expression exist illegal expression
Probability, cause the accuracy of testing result to depend on the expression of detected target.Flow cytometry is that a collection swashs
Optical, electrical muon physics, photoelectric measurement, computer, cell fluorescence chemistry and the cell sorting that is integrated of monoclonal antibody technique and point
Analysis technology, has the analysis advantages such as speed is fast, precision is high, accuracy is good.But its use needs technical professional, with
This simultaneously, general basic hospital and community can not the flow cytometer of purchasing expensive, be difficult to penetration and promotion.Therefore, development
A kind of can be reliable, quick, portable detection Exfoliated tumor cells especially urinary system cancer patient source the tumor that comes off
The equipment of cell is the most necessary.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is to overcome the defect of prior art, it is provided that a kind of urine
Exfoliated tumor cells Dual channel detection equipment, including control device, and be all connected with described control device electric supply installation, drive
Move device, in order to hold the holding apparatus of optical filter and in order to detect the fluorescence detection device of at least 2 different fluorescence signals；
Described driving means is connected with described holding apparatus；Described driving means is in order to drive cutting of optical filter in described holding apparatus
Change；Described control device includes the digital signal processing device controlling described fluorescence detection device；Described fluoroscopic examination fills
Put and include imaging device, the excitation source parts of transmitting optical signal and launch optical signal in order to filter described excitation source parts
Optical filter；Described optical filter is placed between described imaging device and described excitation source parts；Described chip to be measured with described become
As setting position is corresponding.
In some embodiments, described optical filter includes exciting sheet and sheet emitting；Described imaging device excites with described
The inclined angle of light source part is arranged；It is corresponding that described sheet emitting is placed in described imaging device position.
In some embodiments, described excitation source parts fit in the surface exciting sheet.
In some embodiments, described excitation source parts are bonded LED lamp.
In some embodiments, described imaging device is CCD camera；Described CCD camera is in order to by described fluorescent material
The fluorescence optical signals launched is converted to the signal of telecommunication, the more described signal of telecommunication is converted into digital signal, is finally transferred to portable setting
In standby or LCDs, and present with image and corresponding data message form.
In some embodiments, described digital signal processing device include being sequentially connected with in order to gather described numeral letter
Number digital acquisition device, in order to process the digital processing unit of described digital signal, in order to display digit signal processing results
Digital output feature and in order to Digital Signal Processing result to be transferred to the digital transmission unit of mobile terminal.
In some embodiments, apparatus body is also included；Described apparatus body is provided with hollow cavity；Described hollow cavity
Control device, described electric supply installation, described driving means and described holding apparatus have been stated in interior receiving；Described apparatus body is opened
It is provided with the draw-in groove in order to digital output feature described in clamping.In some embodiments, also include being connected with described electric supply installation
Power control, described power control in order to control described electric supply installation switch.
In some embodiments, described CCD camera is connected by digital transmission interface with described digital acquisition device.
In some embodiments, described digital output feature is LCDs；Described apparatus body offers detection
Chip socket.
A kind of urine Exfoliated tumor cells Dual channel detection equipment that the present invention provides is for the beneficial effect of prior art
It is:
Urine Exfoliated tumor cells Dual channel detection equipment, in order to complete the inspection of genitourinary system Exfoliated tumor cells
Survey.This equipment is that the important technology of life sciences, medical science and ecsomatics, biomedical signals measuring and Study on processing method is put down
Platform.There is the features such as special, inexpensive, sensitive, portable.This urine Exfoliated tumor cells Dual channel detection equipment can be integrated in order to examine
The fluoroscopic examination surveying two kinds and above fluorescence signal is integrated, and has huge market-oriented prospect.
It is different that urine Exfoliated tumor cells Dual channel detection equipment can realize detection two kinds on a chip to be checked
Fluorescence signal, it is also possible to simultaneously detect multiple samples, effectively simplifies system design, it is to avoid due to sample optics
The problems such as the inaccuracy that Check-Out Time, difference caused.Cancer information is obtained by detection urine Exfoliated tumor cells, it is achieved
The clinical practices such as the non-invasive diagnosis of cancer, curative effect monitoring.
Additionally, the optical excitation of above-mentioned dual pathways fluorescence detection device and detecting system are compared with prior art, also have
Following advantage:
(1) optical filter in fluorescence detection device, can effectively carry out different fluorescence signal along with the movement of holding apparatus
Recognition detection；
(2) the dual or multiple confirmation of certain tumor cell on same chip to be checked can be realized, it is to avoid because tumor
Cell surface protein differential expression and the problem of flase drop, improve the accuracy of detection.
(3) Dual channel detection device structure designs succinct, compact, is convenient for carrying and moves, and need not specialty
Human users, easy and simple to handle, cost reduces, and greatly reduces the price of tumor cell detection, can be effectively improved urinary system and swell
Tumor examination rate.
In sum, the present invention is directed to the problem that prior art exists, utilize tumor cell mark for detectable substance, set up
A kind of dual pathways fluorescence detection device.This urine Exfoliated tumor cells Dual channel detection equipment and applying at urinary system cancer
The aspects such as disease patient (especially mid-aged population) Exfoliated tumor cells detection, curative effect monitoring have preferable application prospect.
Accompanying drawing explanation
It should be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore it is not construed as model
The restriction enclosed, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to according to these
Accompanying drawing obtains the accompanying drawing that other are relevant.
Fig. 1 is the structural representation of urine Exfoliated tumor cells Dual channel detection equipment of the present invention；
Fig. 2 is the enlarged drawing in Fig. 1 of the present invention.
Drawing reference numeral illustrates:
Detailed description of the invention
Embodiments of the invention are described below in detail, and the example of described embodiment is shown in the drawings, the most from start to finish
Same or similar label represents same or similar element or has the element of same or like function.Below with reference to attached
The embodiment that figure describes is exemplary, it is intended to is used for explaining the present invention, and is not considered as limiting the invention.
In order to be more fully understood that the present invention, embodiments of the invention being described below in detail, the example of described embodiment is attached
Shown in figure, the most same or similar label represents same or similar element or has same or like function
Element.The embodiment described below with reference to accompanying drawing is exemplary, it is intended to be used for explaining the present invention, and it is not intended that
Limitation of the present invention.
In the present invention, unless there are clear and definite restriction, term " mark " should widely understand, such as, " mark " no
It is limited only to p53 albumen, it is also possible to be skin factor growth receptors (EGFR), CD20, CEA, bladder tumor antigen
The tumor cell marks such as BTA.
In the present invention, double fluorescence channels are not limited to GFP and Texas Red, it is also possible to for DAPI, Alexa Fluor@
488, FITC, Cy3, Cy5, Cy7 and Alexa Fluor@750 other fluorescence channels such as grade；
In the present invention, tumor cell dyeing can also use fluorescent dye, and fluorescent dye is not limited to 5-ALA,
Can also be DAPI dyestuff, 7690-XU fluorescent dye, Hoechst33258 dyestuff, acridine orange (AO) etc. other can contaminate tumor
The fluorescent dye of cell.
In the present invention, fluorescent antibody is not limited to Anti-p53Alexa Fluor@488, it is also possible to for Anti-EGFR
Antibody (FITC), it is also possible to for tumor cell primary antibody with common dyeing such as fluorescently-labeled two anti-(such as IgG).
In the present invention, chip material to be checked can be polystyrene, but is not limited to polystyrene, can be also PDMS, also
Can be glass, also can be this patent institute protection domain for PMMA or other materials.
In the present invention, this patent driving means can use all kinds of single-chip microcomputer, ARM, FPGA, Arduino, computer etc.
Realize.
In the present invention, unless otherwise clearly defined and limited, term " install ", " being connected ", " connection ", " fixing " etc.
Term should be interpreted broadly, and connects for example, it may be fixing, it is also possible to be to removably connect, or integral；Can be that machinery connects
Connect, it is also possible to be electrical connection；Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, in can being two elements
The connection in portion or the interaction relationship of two elements.For the ordinary skill in the art, can be according to concrete feelings
Condition understands above-mentioned term concrete meaning in the present invention.
Embodiment
See Fig. 1 and Fig. 2, the invention provides a kind of urine Exfoliated tumor cells Dual channel detection equipment 1, including middle control
Device 11, and be all connected with described control device 11 electric supply installation 13, driving means 14, in order to hold optical filter 163
Holding apparatus 15 and in order to detect the fluorescence detection device 16 of at least 2 different fluorescence signals；Described driving means 14 and institute
State holding apparatus 15 to connect；Described driving means 14 is in order to drive the switching of optical filter 163 in described holding apparatus 15；In described
Control device 11 includes the digital signal processing device controlling described fluorescence detection device 16；Described fluorescence detection device 16 wraps
Include imaging device 161, launch the excitation source parts 162 of optical signal and launch light in order to filter described excitation source parts 162
The optical filter 163 of signal；Described optical filter 163 is placed between described imaging device 161 and described excitation source parts 162；Institute
State chip to be measured corresponding with described imaging device 161 position.
In embodiments of the present invention, described optical filter 163 includes exciting sheet 1631 and sheet emitting 1632；Described imaging device
161 are arranged with the described inclined angle of excitation source parts 162；Described sheet emitting 1632 is placed in described imaging device 161 position
Corresponding.
Above-mentioned, described optical filter 163 includes exciting sheet 1631 and sheet emitting 1632；Described imaging device 161 swashs with described
Luminous source parts 162 are in 45 ° of angles of inclination；Described excite sheet 1631 with described excitation source parts 162 towards consistent；Described
Penetrate sheet 1632 to be placed in immediately below described imaging device 161.
In embodiments of the present invention, described excitation source parts 162 fit in the surface exciting sheet 1631.
In embodiments of the present invention, described excitation source parts 162 are bonded LED lamp.
In embodiments of the present invention, described imaging device 161 is CCD camera；Described CCD camera is in order to send out fluorescent material
The fluorescence optical signals penetrated is converted to the signal of telecommunication, the more described signal of telecommunication is converted into digital signal, is finally transferred to portable set
Or in LCDs, present with image and corresponding data message form.
In embodiments of the present invention, described digital signal processing device include being sequentially connected with in order to gather described numeral letter
Number digital acquisition device, in order to process the digital processing unit of described digital signal, in order to display digit signal processing results
Digital output feature 12 and in order to digital processing results to be transferred to the digital transmission unit of mobile terminal.
In embodiments of the present invention, apparatus body 17 is also included；Described apparatus body 17 is provided with hollow cavity；Described hollow
In cavity, control device 11, described electric supply installation 13, described driving means 14 and described holding apparatus 15 have been stated in receiving；Institute
State apparatus body 17 and offer the draw-in groove 171 in order to digital output feature described in clamping 12.
In embodiments of the present invention, the power control 18 being connected with described electric supply installation 13, described power supply are also included
Control device 18 to switch in order to control described electric supply installation 13.
In embodiments of the present invention, described CCD camera is connected by digital transmission interface with described digital acquisition device.
In embodiments of the present invention, described digital output feature 12 is LCDs；Described apparatus body 17 offers
Detection chip socket 172.
Above-mentioned, that a kind of urine Exfoliated tumor cells Dual channel detection equipment 1 is specifically applied embodiment, i.e. detects urinary system
Exfoliated tumor cells in system tumor patient urine specimen.
Concrete components and parts are chosen:
Green fluorescence channel optical filter, excites sheet 1631, peak wavelength 488nm, sheet emitting 1632, peak wavelength 507nm,
Bandwidth 10nm；
Red fluorescence channel optical filter, excites sheet 1631, peak wavelength 594nm, sheet emitting 1632, peak wavelength 617nm,
Bandwidth 10nm；
Light source: LED forward conduction electric current is 5mA, forward conduction voltage is 2.8V；Described external power supply voltage is 12V；
Its operating procedure is as follows:
1, the acquisition of Exfoliated tumor cells in urine specimen
1. bladder cancer patients urina sanguinis mud-stream urine 10-50mL is collected.
2. with micro-fluid chip, urine is carried out filtration treatment, in order to separate, to be enriched with Exfoliated tumor cells, use after filtration
The PBS of pH 7.4 is rinsed, and goes the removal of impurity.
2, fluorescent dyeing
1. utilize FAST, the Exfoliated tumor cells of institute's separation and concentration is dyeed.
Wherein, fluorescent antibody selection Anti-p53Alexa488 and Anti-EGFR antibody
(Alexa647)。
Wherein, the order processed tumor cell is followed successively by fixing 10min, the Triton X-100 of paraformaldehyde (4%)
(0.1%) transparent processing 5min, BSA (1%) room temperature closes 1h, the dyeing of fluorescent antibody 4 DEG C overnight (or 37 DEG C of 1h).
3, dual pathways fluoroscopic examination
Chip to be checked (containing through hyperchromatic Exfoliated tumor cells) is inserted the chip porch of detection equipment.
Turning on the power controller, detection equipment is started working, and LED emission goes out light through exciting sheet 1631 to enter fluorescent material
Row excites, and fluorescent material absorbs exciting light and launches green fluorescence (Anti-p53Alexa488 coloration results), logical
Cross after sheet emitting 1632 filters and captured by CCD.It is bright that one Exfoliated tumor cells is shown as a green under green fluorescence channel
Point, all effective green bright spots complete to be dyed to the counting of green cell after digital signal processing device processes；Same with this
Time, green light signal image in Bluetooth transmission to portable set or PC, with the form of image and corresponding data message in
Reveal to come.
Closedown green fluorescence channel switchs, and motor drives red fluorescence optical filter to sense channel, opens red glimmering
Fibre channel switch, equally, excites sheet 1631 to be irradiated to red fluorescent material after filtering, and fluorescent material launches red fluorescence
(Anti-EGFR antibody coloration result) is captured by CCD after being filtered by sheet emitting 1632 again.One Exfoliated tumor cells exists
Being shown as a bright red spot under red fluorescence channel, all effective bright red spots equally can be by digital signal processing device
Manage and complete to be dyed to the counting of red cell；Meanwhile, red light signal image through Bluetooth transmission to portable set or
On PC, present with the form of image and corresponding data message equally.Follow-up can general on portable set (such as PC) is schemed
Being overlapped in ImageJ image analysis software processing as importing, color is yellow, and it is folded to use analysis of accounts function to count
The bright spot number added, completes detection.
4, take out detection chip, power off.
Above-mentioned, it is to be understood that urine Exfoliated tumor cells Dual channel detection equipment 1, can be by through fluorescence staining
Tumor cell quickly identifies and counts, and has the features such as special, cheap, sensitive, portable, has huge market-oriented prospect.
It is to be appreciated that Exfoliated tumor cells can be with stable existence in urine, especially urine sample can be non-
Obtain in a large number on the premise of traumatic damage.Therefore, urine Exfoliated tumor cells separating through chip, be enriched with, dyeing processes
After, can be directly transferred in Dual channel detection equipment detect on mobile platform.
It is to be appreciated that fluorescence detection channel can be the dual pathways, it is also possible to for triple channel or multichannel.
It is to be appreciated that it is that detectable substance carries out fluorescence staining that this patent chooses tumor cell surface mark, can be special
Property detection urine Exfoliated tumor cells, to genitourinary system tumor patient's Exfoliated tumor cells detection and Treatment monitoring have weight
The meaning wanted.The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a urine Exfoliated tumor cells Dual channel detection equipment, it is characterised in that: include control device, and all with described
Control device connect electric supply installation, driving means, in order to hold the holding apparatus of optical filter and in order to detect at least 2 not
Fluorescence detection device with fluorescence signal；Described driving means is connected with described holding apparatus；Described driving means is in order to drive
The switching of optical filter in described holding apparatus；Described control device includes the digital signal controlling described fluorescence detection device
Processing means；Described fluorescence detection device includes imaging device, excitation source parts launching optical signal and described in order to filter
Excitation source parts launch the optical filter of optical signal；Described optical filter be placed in described imaging device and described excitation source parts it
Between；Chip to be measured is corresponding with described imaging device position.
2. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 1, it is characterised in that: described optical filtering
Sheet includes exciting sheet and sheet emitting；Described imaging device is arranged with the described inclined angle of excitation source parts；Described sheet emitting
It is placed in described imaging device position corresponding.
3. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 2, it is characterised in that excite described in:
Light source part fits in the surface exciting sheet.
4. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 3, it is characterised in that excite described in:
Light source part is bonded LED lamp.
5. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 1, it is characterised in that: described imaging
Device is CCD camera；Described CCD camera in order to be converted to the signal of telecommunication by the fluorescence optical signals that fluorescent material is launched, then by described
The signal of telecommunication is converted into digital signal, is finally transferred in portable set or LCDs, and with image and corresponding data
Message form presents.
6. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 5, it is characterised in that: described numeral
Signal processing apparatus include being sequentially connected with in order to gather the digital acquisition device of described digital signal, in order to process described numeral
The digital processing unit of signal, in order to the digital output feature of display digit signal processing results and in order to digital processing is tied
Fruit is transferred to the digital transmission unit of mobile terminal.
7. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 6, it is characterised in that: also include setting
Standby body；Described apparatus body is provided with hollow cavity；In described hollow cavity, control device, described confession Denso have been stated in receiving
Put, described driving means and described holding apparatus；Described apparatus body offers in order to digital output feature described in clamping
Draw-in groove.
8. urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 1 a kind of, it is characterised in that: also include with
The power control that described electric supply installation connects, described power control is in order to control described electric supply installation switch.
9. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 6, it is characterised in that: described CCD
Camera is connected by digital transmission interface with described digital acquisition device.
10. a kind of urine Exfoliated tumor cells Dual channel detection equipment as claimed in claim 7, it is characterised in that: described number
Word output device is LCDs；Described apparatus body offers detection chip socket.
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| CN201610792664.5A CN106248942A (en) | 2016-08-31 | 2016-08-31 | A kind of urine Exfoliated tumor cells Dual channel detection equipment |
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| CN201610792664.5A CN106248942A (en) | 2016-08-31 | 2016-08-31 | A kind of urine Exfoliated tumor cells Dual channel detection equipment |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911860A (en) * | 2012-09-29 | 2013-02-06 | 武汉斯坦姆赛尔生物技术有限公司 | Portable fluorecyte counting device |
| CN205484055U (en) * | 2016-02-18 | 2016-08-17 | 苏州晋翌生物医学仪器有限公司 | Fluorescence detection system and multichannel detection device |
| CN206002548U (en) * | 2016-08-31 | 2017-03-08 | 隗慧林 | A kind of urine Exfoliated tumor cells Dual channel detection equipment |
-
2016
- 2016-08-31 CN CN201610792664.5A patent/CN106248942A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911860A (en) * | 2012-09-29 | 2013-02-06 | 武汉斯坦姆赛尔生物技术有限公司 | Portable fluorecyte counting device |
| CN205484055U (en) * | 2016-02-18 | 2016-08-17 | 苏州晋翌生物医学仪器有限公司 | Fluorescence detection system and multichannel detection device |
| CN206002548U (en) * | 2016-08-31 | 2017-03-08 | 隗慧林 | A kind of urine Exfoliated tumor cells Dual channel detection equipment |
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Application publication date: 20161221 |