CN106244508A - One strain Burkholderia pyrrocinia and application thereof - Google Patents
One strain Burkholderia pyrrocinia and application thereof Download PDFInfo
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- CN106244508A CN106244508A CN201610880271.XA CN201610880271A CN106244508A CN 106244508 A CN106244508 A CN 106244508A CN 201610880271 A CN201610880271 A CN 201610880271A CN 106244508 A CN106244508 A CN 106244508A
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Abstract
The present invention relates to strain Burkholderia pyrrocinia and an application thereof, belong to environmental microorganism application.The Burkholderia pyrrocinia B1213 of the present invention, deposit number is CGMCCNo.12806；The preservation time is on July 21st, 2016；Belong to shaft-like gram negative bacteria, it is impossible to form spore.The Burkholderia pyrrocinia B1213 of the present invention can degrade dibutyl phthalate；The degradation rate of dibutyl phthalate be may be up to 45.5 75.35%.The method of the Burkholderia pyrrocinia B1213 degraded dibutyl phthalate of the employing present invention is: under conditions of yeast extract exists, Burkholderia pyrrocinia B1213 with DBP contacts.The present invention degrades the method for dibutyl phthalate, compared with the method for antibacterial, fungus degrading dibutyl phthalate before the present invention, has new meaning on strain source, and degradation rate is high, and degradation cycle is short, simple to operate.
Description
Technical field
The present invention relates to strain Burkholderia pyrrocinia and an application thereof, belong to environmental microorganism application.
Background technology
Dibutyl phthalate (Dibutyl phthalate), is called for short: DBP, belongs to phthalic acid ester
The one of (Phhtalic Aicd Easters, PAEs), is the important environmental hormone class organic synthesis compound of a class, has
The features such as lighter color, volatility are low, abnormal smells from the patient is little and low temperature resistant, are the plasticizers that yield is maximum in recent years, consumption is most, extensively use
In industries such as rubber, plastics, spice.
DBP is connected instability with carrier, is easily diffused in environment, can pass through food, air, drinking water, cosmetics etc.
Number of ways enters human body and is enriched with.DBP has poisonous effect to water plant, has animal estrogen and interferes significantly with work
With, the expression of surface of cell membrane albumen can be reduced thus suppress the phagocytic activity of macrophage, even inducing nerve cell apoptosis,
It is a kind of important environment incretion interferent and carcinogenic, teratogenesis, mutagenic matter, causes the height weight of environmental administration of various countries
Depending on.EPA (EPA), European Union and China National Environmental Monitoring Center have been listed in the black name of priority pollutants the most
Single.China the most correspondingly defines the maximum concentrations of DBP in Drinking Water.
In environment, the decomposition of DBP, method for transformation and technology exploration have become the important research direction of environmental pollution improvement.But
Be DBP hydrolysis in natural environment, photodissociation speed slowly, belong to hard-degraded substance.There are some researches show, the aqueous phase of BBP
Middle Photolysis Half is longer than 100 days.Compared with hydrolysis and the mechanical degradation such as photodissociation, biodegradation process based on microbial metabolism
There is the feature such as functional microorganism multiformity, process simple, low cost, environmental friendliness so that microbial degradation is considered as certainly
So most effective approach of DBP permineralization in environment.Therefore micro-life of the efficient-decomposition PAEs class material with DBP as representative is screened
Thing also illustrates its catabolic pathway, has realistic meaning for in-depth about the research and application eliminating DBP environmental hazard.
In recent years, the microbial degradation of DBP is studied the most widely, and the bacterial strain of energy efficient degradation DBP is in a large number
Isolated from all kinds of environment, including the activated sludge etc. of Rhizophora apiculata Blume, soil, ocean, river and waste water treatment plant, microorganism
Classification includes a large amount of antibacterial and some fungi.Research shows, separate sources and different types of microorganism are on DBP degraded way
Footpath aspect has larger difference, and the degradation mechanism presented also is not quite similar.Described degradation mechanism includes playing Degradation
Key enzyme and enzyme system, degradation pathway in key node and key influence factor, microorganism own metabolism and degradation effect it
Between relation, microbial degradation broad spectrum activity and specificity and the relation etc. with DBP structure thereof.
Burkholderia pyrrocinia (Burkholderia pyrrocinia), its application is mainly promoting plant growing
Biological control with plant disease；Have no the report about utilizing Burkholderia pyrrocinia degraded DBP at present.
Summary of the invention
It is an object of the invention to provide the new strains of a kind of dibutyl phthalate of can degrading；And this bacterial strain should
By the method with this strains for degrading dibutyl phthalate of employing.
Technical scheme
The present invention screens from soil and has isolated the bacterial strain that a strain is new；Belong to shaft-like gram negative bacteria, it is impossible to formed
Spore；Identified, this bacterial strain be a kind of Burkholderia pyrrocinia (Burkholderia pyrrocinia)；Named pyrrole
Cough up bulkholderia cepasea B1213；It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center；Preservation is compiled
Number it is CGMCCNo.12806；The preservation time is on July 21st, 2016.
The Burkholderia pyrrocinia B1213 of the present invention can degrade dibutyl phthalate；Primary gram of Hall of pyrroles
Moral Salmonella B1213 may be up to 45.5-75.35% to the degradation rate of dibutyl phthalate.
Use the one of which method of the Burkholderia pyrrocinia B1213 degraded dibutyl phthalate of the present invention
For: under conditions of yeast extract exists, Burkholderia pyrrocinia B1213 contacts with dibutyl phthalate.Its
In, the amount of yeast extract can affect degradation rate.So, said method, it is preferred that at improvement BSM basis salt fluid medium
Under conditions of existence, Burkholderia pyrrocinia B1213 contacts with dibutyl phthalate；Described improvement BSM basis salt
Fluid medium, containing following component in every 1L: yeast extract 2-10g, ammonium sulfate 0.5g, sodium chloride 4.0g, three are hydrated phosphorus
Acid potassium 0.5g, bitter salt 0.4g, distilled water surplus；pH7.0.
Concrete, it is that dibutyl phthalate is added improvement BSM basis salt fluid medium；By pyrroles Bai Kehuo
Your moral Salmonella B1213 seed liquor is inoculated in improvement BSM basis salt fluid medium, the condition of 150-200 rpm, 30-35 DEG C
Lower isothermal vibration is cultivated.
Said method, it is preferred that in the salt fluid medium of improvement BSM basis, the content of yeast extract is 7g/L.
Said method, it is preferred that condition of culture is rotating speed 175rpm, temperature 30 DEG C.Under other identical conditions, at this
Under condition of culture, the degradation rate of dibutyl phthalate is higher.
Said method, Burkholderia pyrrocinia B1213 seed liquor is relative to improvement BSM basis salt fluid medium
The change of inoculum concentration, the dibutyl phthalate degradation rate in the unit interval is had a significant effect；Under normal circumstances, inoculation
Measuring the most, in the unit interval, degradation rate is the highest；But to final degradation rate, (in fermentation liquid, dibutyl phthalate content reaches
Degradation rate when stablizing) have not significant impact.
Said method, described Burkholderia pyrrocinia B1213 seed liquor is by Burkholderia pyrrocinia B1213
Cultivate acquisition.Under conditions of obtaining Burkholderia pyrrocinia B1213, those skilled in the art by routine operation are
Burkholderia pyrrocinia B1213 seed liquor can be obtained.
In the present invention, for the percentage ratio of the content of certain composition, if not otherwise specified, refer both to percentage by weight
(w/w).
Technical term explanation used by the present invention: rpm is Speed unit, and 1 rpm refers to that rotation per minute is gone around.
Beneficial effect:
(1) separation and Culture goes out the Burkholderia pyrrocinia B1213 of dibutyl phthalate of can degrading first；
(2) Burkholderia pyrrocinia B1213 is 45.5%-75.35% to the degradation rate of dibutyl phthalate；
(3) present invention degrades antibacterial, fungus degrading phthalic acid two before the method for dibutyl phthalate, with the present invention
The method of butyl ester is compared, and has new meaning on strain source, and degradation rate is high, and degradation cycle is short, simple to operate.
Preservation information:
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center；
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica；
Preservation date: on July 21st, 2016；
Deposit number: CGMCCNo.12806；
Classification And Nomenclature: Burkholderia pyrrocinia (Burkholderia pyrrocinia).
Accompanying drawing explanation
Fig. 1 is the Burkholderia pyrrocinia B1213 morphological characteristic under the microscope of the present invention；In Fig. 1, Bi Kabai
Ke Huoerde Salmonella B1213 is shaft-like gram negative bacteria, it is impossible to form spore；
Fig. 2 is the DBP degradation effect of the embodiment 2 recorded by high performance liquid chromatography；In Fig. 2: a figure is DBP blank
HPLC-UV detection, the HPLC-UV detection that b figure is DBP after degraded；DBP goes out peak at 7.825min substantially, degraded
After, the peak area of DBP reduces；
Fig. 3 is the 16SrDNA sequential system developmental analysis of Burkholderia pyrrocinia B1213；
Fig. 4 is that the recA sequential system of Burkholderia pyrrocinia B1213 grows tree analysis.
Detailed description of the invention
In following embodiment, if no special instructions, it is method commonly used in the art.Agents useful for same or the unreceipted production of instrument
Manufacturer person, be can by city available from conventional products.
The qualification of embodiment 1 bacterial strain B1213
Bacterial strain B1213 is isolatable from soil, and its form is as it is shown in figure 1, belong to shaft-like gram negative bacteria, it is impossible to form spore.
Extract its genomic DNA, and utilize 16s rDNA and BCR1 primer to carry out polymerase chain reaction for this genomic DNA
(Polymerase Chain Reaction, PCR) is to breed specific DNA sequence, and utilizes American National Biotechnology Information
Center (National Center for Biotechnology Information is called for short NCBI) data base carries out bacterial strain ratio
Right.
(1) 16S rDNA sequence analysis:
16s rDNA forward primer 27F:5 '-AGA GTT TGA TCC TGG CTC AG-3 ', as shown in SEQ ID NO.1；
16s rDNA reverse primer 1492R:5'-ACG GTT ACC TTG TTA CGA CTT-3', such as SEQ ID NO.2 institute
Show；
Amplification gained 16S rDNA sequence is as shown in SEQ ID NO:3, and sequence is 1411bp.By this amplification gained 16S
RDNA sequence compares with the gene order of the related strain in GenBank data base, carries out sequence ratio with MEGA4.1 software
Right, use and face a connection method phylogenetic tree construction, through 1000 stochastic sampling, calculate Bootstrap value, constructed system
Grow tree such as Fig. 3.Figure is grown tree node and only shows that Bootstrap value is more than 50% numerical value, upper target " T " intermediate scheme bacterium
Strain.Bacterial strain " B1213 " and type strain Burkholderia stabilis LMG 28156, homology can be found from NCBI
Property reaches 99.7%.
(2) recA gene sequencing
BCR1 gene forward primer: 5'-TgA CCg CCg AgA AgA gCA A-3', as shown in SEQ ID NO.4；
BCR2 gene reverse primer: 5'-CTC TTC TTC gTC CAT CgC CTC-3', as shown in SEQ ID NO.5；
Amplification gained recA gene order is as shown in SEQ ID NO:6, and sequence is 974bp.By this amplification gained recA base
Because the gene order of sequence with the related strain in GenBank data base compares, carry out sequence ratio with MEGA4.1 software
Right, use and face a connection method phylogenetic tree construction, through 1000 stochastic sampling, calculate Bootstrap value, constructed system
Grow tree such as Fig. 4.Figure is grown tree node and only shows that Bootstrap value is more than 50% numerical value, upper target " T " intermediate scheme bacterium
Strain.Bacterial strain " B1213 " and type strain Burkholderia pyrrocinia DSM 10685T can be found from NCBI
(CP011503) homology reaches 97.9%.
Therefore can be determined that B1213 is a kind of brand-new Burkholderia pyrrocinia.Bacterium belonging to it is confirmed through identifying
After Zhong, Burkholderia pyrrocinia B1213 is preserved in China Committee for Culture Collection of Microorganisms on July 21st, 2016
Common micro-organisms center；Deposit number is CGMCCNo.12806；This biomaterial survival test is tested and passes through this examination
Test.
Prepared by embodiment 2 Burkholderia pyrrocinia B1213 liquid submerged culture liquid
(1) slant culture: Burkholderia pyrrocinia B1213 is inoculated on slant medium, cultivates 48 hours at 40 DEG C,
Obtain inclined-plane bacterial strain.Slant medium used, the composition contained in every 1L is: glucose 2.0g, peptone 1.0g, yeast extract
0.5g, agar 1.8g, distilled water surplus, pH is neutral, 115 DEG C of sterilizing 20 min.
(2) inclined-plane inoculation is taken in sterilized seed culture medium, in 175 rpm, constant temperature shake under conditions of 30 DEG C
Swing cultivation 24h, obtain seed culture fluid.Seed culture medium used, the composition contained in every 1L is: ammonium sulfate 0.5g, sodium chloride
4.0g, three hypophosphite monohydrate potassium 0.5g, bitter salt 0.4g, agar powder 15g, yeast extract 5g, distilled water surplus,
PH7.0,121 DEG C of sterilizing 25min.
(3) DBP (technical pure) of 10 μ L is added the improvement BSM basis salt liquid culture containing 50mL with sterile working
In the shaking flask of base, the seed culture fluid of Burkholderia pyrrocinia B1213 is inoculated in shaking flask with the inoculum concentration of 1mL,
175r/min, isothermal vibration cultivates 72h under conditions of 30 DEG C；Obtain fermentation liquid.Improvement BSM basis used salt fluid medium, often
The composition contained in 1L is: yeast extract 5g, ammonium sulfate 0.5g, sodium chloride 4.0g, three hypophosphite monohydrate potassium 0.5g, seven hydration sulfur
Acid magnesium 0.4g, distilled water surplus, pH7.0,121 DEG C of sterilizing 15min.
Embodiment 3 DBP standard curve is formulated and assay
(1) high performance liquid chromatography (HPLC) makes standard curve: takes 600 μ L DBP with methanol as solvent, is prepared as 1mg/mL
DBP solution, take 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.2mL, 1.4mL respectively after diluting ten times and be settled to 2mL
In centrifuge tube, i.e. Concentraton gradient (μ g/mL) is 10,20,30,40,50,60,70 respectively with after the 0.45 organic membrane filtration of μm
It is placed in liquid phase bottle to be measured.Efficient liquid phase testing conditions in the present embodiment 3 is: chromatographic column is Sepax Gp-C18 post
(150mm*4.6mm, 5.0 μm)；Flowing is acetonitrile-water (83:17, V/V) mutually；Sample size 10 μ L；Flow velocity 1.0mL/min；Column temperature
25C°；UV-detector wavelength 210nm.With the peak area of DBP as abscissa, with the concentration of DBP as vertical coordinate, make standard bent
Line；And then obtain peak area-concentration equation.
The degradation rate of embodiment 4 high-efficient liquid phase technique detection DBP
(1) in the fermentation liquid that embodiment 2 obtains, add the acetonitrile with fermentation liquid equivalent DBP is extracted, ultrasonic
Take wherein 2mL acetonitrile after (40KHZ, 300W) assisted extraction 30min and be settled to 10mL, centrifugal after mix homogeneously
(12000rpm, 10min), filters the supernatant after centrifugal with organic filter membrane (0.45 μm), discards just filtrate, takes continuous filter
Liquid is placed in liquid phase bottle, with the testing conditions of embodiment 3 step (1), uses high performance liquid chromatograph (HPLC) to detect.
The operation time is 20 minutes, reads the retention time peak area value at about 7 min, according to the peak area-concentration of embodiment 3
Equation, obtains the concentration of DBP in subsequent filtrate, and then calculates the residual concentration of DBP in fermentation liquid.
(2) computing formula of degradation rate: degradation rate %=(C0-C)/C0*100%, C0 is for using Burkholderia pyrrocinia
DBP mass concentration (μ g/mL) before B1213 degraded (the i.e. DBP of mixed liquor in shaking flask before embodiment 2 step (3) fermentation
Mass concentration: 0.1713 μ g/mL), C is the DBP residual concentration (μ g/mL) in fermentation liquid.It is computed, the degraded of embodiment 2
Rate is 55.96%.
The optimization of embodiment 5 Burkholderia pyrrocinia B1213 degraded DBP
Seed liquor inoculum concentration in embodiment 2 step (3) is changed into successively 2,3,4,5,6mL, other operations are with embodiment 2.Press
According to the DBP concentration of the step measurements fermentation liquid of embodiment 4, and then calculate the degradation rate of DBP.Under the conditions of different vaccination amount, DBP
Degradation rate is as shown in table 1.
Embodiment 6
The content of the yeast extract of improvement BSM basis used salt fluid medium in embodiment 2 step (3) is revised successively
Be 2,3,4,5,6,7,8,10g/L；Other operations are with embodiment 2.According to the DBP concentration of the step measurements fermentation liquid of embodiment 4,
And then calculate the degradation rate of DBP.Under the conditions of the content of different yeast extracts, DBP degradation rate is as shown in table 2.
Table 1
| Seed liquor inoculum concentration (mL) | DBP degradation rate (%) |
| 0 | 0 |
| 1 | 55.96 |
| 2 | 56.52 |
| 3 | 58.95 |
| 4 | 63.51 |
| 5 | 69.64 |
| 6 | 75.35 |
；
Table 2
| The content (g/L) of yeast extract | DBP degradation rate (%) |
| 0 | 0 |
| 2 | 45.5 |
| 3 | 45.8 |
| 4 | 46.32 |
| 5 | 55.96 |
| 6 | 65.32 |
| 7 | 75.35 |
| 8 | 69.84 |
| 10 | 67.78 |
<110>Beijing Technology and Business University
<120>one strain Burkholderia pyrrocinia and application thereof
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AGAGTTTGAT CCTGGCTCAG 20
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<400>2
ACGGTTACCT TGTTACGACT T 21
<210>3
<211>1411
<212>DNA
<213>synthetic
<400>3
ACATGCAGTC GAACGGCAGC ACGGGTGCTT GCACCTGGTG GCGAGTGGCG AACGGGTGAG 60
TAATACATCG GAACATGTCC TGTAGTGGGG GATAGCCCGG CGAAAGCCGG ATTAATACCG 120
CATACGATCT ACGGATGAAA GCGGGGGACC TTCGGGCCTC GCGCTATAGG GTTGGCCGAT 180
GGCTGATTAG CTAGTTGGTG GGGTAAAGGC CTACCAAGGC GACGATCAGT AGCTGGTCTG 240
AGAGGACGAC CAGCCACACT GGGACTGAGA CACGGCCCAG ACTCCTACGG GAGGCAGCAG 300
TGGGGAATTT TGGACAATGG GCGAAAGCCT GATCCAGCAA TGCCGCGTGT GTGAAGAAGG 360
CCTTCGGGTT GTAAAGCACT TTTGTCCGGA AAGAAATCCT TGGCTCTAAT ACAGTCGGGG 420
GATGACGGTA CCGGAAGAAT AAGCACCGGC TAACTACGTG CCAGCAGCCG CGGTAATACG 480
TAGGGTGCGA GCGTTAATCG GAATTACTGG GCGTAAAGCG TGCGCAGGCG GTTTGCTAAG 540
ACCGATGTGA AATCCCCGGG CTCAACCTGG GAACTGCATT GGTGACTGGC AGGCTAGAGT 600
ATGGCAGAGG GGGGTAGAAT TCCACGTGTA GCAGTGAAAT GCGTAGAGAT GTGGAGGAAT 660
ACCGATGGCG AAGGCAGCCC CCTGGGCCAA TACTGACGCT CATGCACGAA AGCGTGGGGA 720
GCAAACAGGA TTAGATACCC TGGTAGTCCA CGCCCTAAAC GATGTCAACT AGTTGTTGGG 780
GATTCATTTC CTTAGTAACG TAGCTAACGC GTGAAGTTGA CCGCCTGGGG AGTACGGTCG 840
CAAGATTAAA ACTCAAAGGA ATTGACGGGG ACCCGCACAA GCGGTGGATG ATGTGGATTA 900
ATTCGATGCA ACGCGAAAAA CCTTACCTAC CCTTGACATG GTCGGAATCC CGCTGAGAGG 960
TGGGAGTGCT CGAAAGAGAA CCGATACACA GGTGCTGCAT GGCTGTCGTC AGCTCGTGTC 1020
GTGAGATGTT GGGTTAAGTC CCGCAACGAG CGCAACCCTT GTCCTTAGTT GCTACGCAAG 1080
AGCACTCTAA GGAGACTGCC GGTGACAAAC CGGAGGAAGG TGGGGATGAC GTCAAGTCCT 1140
CATGGCCCTT ATGGGTAGGG CTTCACACGT CATACAATGG TCGGAACAGA GGGTTGCCAA 1200
CCCGCGAGGG GGAGCTAATC CCAGAAAACC GATCGTAGTC CGGATTGCAC TCTGCAACTC 1260
GAGTGCATGA AGCTGGAATC GCTAGTAATC GCGGATCAGC ATGCCGCGGT GAATACGTTC 1320
CCGGGTCTTG TACACACCGC CCGTCACACC ATGGGAGTGG GTTTTACCAG AAGTGGCTAG 1380
TCTAACCGCA AGGAGGACGG TCACCACGGT A 1411
<210>4
<211>19
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TgACCgCCgA gAAgAgCAA 19
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CTCTTCTTCg TCCATCgCCT C 21
<210>6
<211>974
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<213>synthetic
<400>6
TCATGCGCAT GGGCGACGGC GAGGCGGCCG AAGACATCCA GGTCGTCTCC ACGGGTTCGC 60
TGGGGCTCGA CATCGCGCTT GGCGTCGGCG GCTGCCGCGC GGCCGGGTGG TCGAGATCTA 120
CGGCCCGGAA TCGTCCGGTA AAACCACGCT CACGCTGCAG GTCATCGCCG AACTGCAGAA 180
GATCGGCGGC ACGGCCGCGT TCATCGACGC CGAACACGCG CTCGACGTTC AATATGCGTC 240
GAAGCTCGGC GTGAACGTGC CGGAACTGCT GATCTCGCAG CCGGACACCG GCGAACAGGC 300
ACTGGAAATC ACCGATGCGC TGGTGCGCTC GGGCTCGGTC GACATGATCG TCATCGACTC 360
GGTCGCGGCG CTCGTGCCGA AGGCCGAAAT CGAAGGCGAG ATGGGCGATT CGCTGCCGGG 420
CCTGCAGGCT CGCCTGATGT CGCAGGCGCT GCGCAAGCTG ACCGGCACGA TCAAGCGCAC 480
GAACTGCCTG GTGATCTTCA TCAACCAGAT TCGCATGAAG ATCGGCGTGA TGTTCGGCAA 540
CCCGGAAACC ACGACGGGCG GCAACGCGCT GAAGTTCTAT GCGTCGGTGC GTCTCGATAT 600
CCGCCGGATC GGCTCGATCA AGAAGAACGA CGAGGTGATC GGCAACGAAA CCCGCGTGAA 660
GGTCGTCAAG AACAAGGTGT CGCCGCCGTT CCGCGAAGCG ATCTTCGACA TCCTGTATGG 720
CGAGGGCATT TCGCGTCAGG GCGAGATCAT CGATCTCGGC GTGCAGGCAA AGATCGTCGA 780
CAAGGCGGGC GCCTGGTACA GCTACAACGG CGAGAAGATC GGCCAGGGCA AGGACAACGC 840
GCGTGAATTC CTGCGCGAGA ATCCGGAAAT CGCACGCGAG ATCGAAAACC GCATCCGCGA 900
ATCGCTCGGC GTCGTCGCCA AGGCGCTGGC GGCCGCACTC GCGCAGATCG AGAAGCAGTT 960
CGGCAAAGGG TCGA 974
Claims (9)
1. a strain Burkholderia pyrrocinia (Burkholderia pyrrocinia) B1213, it is preserved in China Microbiological bacterium
Plant preservation administration committee common micro-organisms center；Deposit number is CGMCCNo.12806；The preservation time is July 21 in 2016
Day.
The most according to claim 1, Burkholderia pyrrocinia B1213, belong to shaft-like gram negative bacteria, it is impossible to shape
Become spore.
3. a purposes of Burkholderia pyrrocinia B1213 described in claim 1 or 2, is used for phthalic acid two of degrading
Butyl ester.
4. one kind uses the side of Burkholderia pyrrocinia B1213 degraded dibutyl phthalate described in claim 1 or 2
Method: under conditions of yeast extract exists, Burkholderia pyrrocinia B1213 contacts with dibutyl phthalate.
Method the most according to claim 4, it is characterised in that in the condition that improvement BSM basis salt fluid medium exists
Under, Burkholderia pyrrocinia B1213 contacts with dibutyl phthalate；Described improvement BSM basis salt fluid medium,
Containing following component in every 1L: yeast extract 2-10g, ammonium sulfate 0.5g, sodium chloride 4.0g, three hypophosphite monohydrate potassium 0.5g, seven
Magnesium sulfate heptahydrate 0.4g, distilled water surplus；pH7.0.
Method the most according to claim 5, it is characterised in that yeast extract in the salt fluid medium of improvement BSM basis
Content be 7g/L.
7. according to the method described in claim 4,5 or 6, it is characterised in that be that dibutyl phthalate is added improvement BSM
Basis salt fluid medium；Burkholderia pyrrocinia B1213 seed liquor is inoculated in improvement BSM basis salt liquid culture
Base, under conditions of 150-200rpm, 30-35 DEG C, isothermal vibration is cultivated.
Method the most according to claim 7, it is characterised in that rotating speed 175rpm, temperature 30 DEG C.
Method the most according to claim 8, it is characterised in that described Burkholderia pyrrocinia B1213 seed liquor is
Acquisition is cultivated by Burkholderia pyrrocinia B1213.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610880271.XA CN106244508B (en) | 2016-10-09 | 2016-10-09 | One plant of Burkholderia pyrrocinia and its application |
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| CN106591201A (en) * | 2017-01-04 | 2017-04-26 | 华南农业大学 | Degradation strain of fungicide pyraclostrobin, microbial inoculum prepared from degradation strain and application of degradation strain |
| CN106701636A (en) * | 2017-01-23 | 2017-05-24 | 南京林业大学 | Application of burkholderia pyrrocinia JK-SH007 bacterial strain as siderophore high-yield bacterial strain |
| CN110591965A (en) * | 2019-09-29 | 2019-12-20 | 北京工商大学 | Burkholderia polyphylla culture method and application thereof in catalytic synthesis of white spirit flavor ester and degradation of white spirit harmful ester |
| CN111394382A (en) * | 2020-04-22 | 2020-07-10 | 北京工商大学 | Recombinant expression vector and recombinant bacterium of feruloyl esterase BpFae gene, and recombinant expression method |
| CN111471664A (en) * | 2020-04-22 | 2020-07-31 | 北京工商大学 | Feruloyl esterase BpFae, and coding gene and application thereof |
| CN113699068A (en) * | 2021-08-26 | 2021-11-26 | 杭州市农业科学研究院 | Burkholderia pyrrocinia strain and application thereof |
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| CN106591201A (en) * | 2017-01-04 | 2017-04-26 | 华南农业大学 | Degradation strain of fungicide pyraclostrobin, microbial inoculum prepared from degradation strain and application of degradation strain |
| CN106591201B (en) * | 2017-01-04 | 2019-07-19 | 华南农业大学 | A kind of degradation bacteria strains of fungicide pyraclostrobin and its microbial inoculum and the application of production |
| CN106701636A (en) * | 2017-01-23 | 2017-05-24 | 南京林业大学 | Application of burkholderia pyrrocinia JK-SH007 bacterial strain as siderophore high-yield bacterial strain |
| CN110591965A (en) * | 2019-09-29 | 2019-12-20 | 北京工商大学 | Burkholderia polyphylla culture method and application thereof in catalytic synthesis of white spirit flavor ester and degradation of white spirit harmful ester |
| CN112980722A (en) * | 2019-09-29 | 2021-06-18 | 北京工商大学 | Burkholderia polyphylla culture method and application thereof in catalytic degradation of harmful esters of white spirit |
| CN112980722B (en) * | 2019-09-29 | 2022-10-14 | 北京工商大学 | Method for culturing Burkholderia polyphylla and application of Burkholderia polyphylla in catalytic degradation of harmful esters of white spirit |
| CN111394382A (en) * | 2020-04-22 | 2020-07-10 | 北京工商大学 | Recombinant expression vector and recombinant bacterium of feruloyl esterase BpFae gene, and recombinant expression method |
| CN111471664A (en) * | 2020-04-22 | 2020-07-31 | 北京工商大学 | Feruloyl esterase BpFae, and coding gene and application thereof |
| CN111394382B (en) * | 2020-04-22 | 2021-11-05 | 北京工商大学 | Recombinant expression vector and recombinant bacterium of feruloyl esterase BpFae gene, and recombinant expression method |
| CN111471664B (en) * | 2020-04-22 | 2021-11-30 | 北京工商大学 | Feruloyl esterase BpFae, and coding gene and application thereof |
| CN113699068A (en) * | 2021-08-26 | 2021-11-26 | 杭州市农业科学研究院 | Burkholderia pyrrocinia strain and application thereof |
| CN113699068B (en) * | 2021-08-26 | 2023-02-03 | 杭州市农业科学研究院 | Burkholderia pyrrocinia strain and application thereof |
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