CN106244502B - The pseudomonad of one plant of efficient dephosphorization and degradation lecithin - Google Patents
The pseudomonad of one plant of efficient dephosphorization and degradation lecithin Download PDFInfo
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Abstract
The invention discloses one plant of efficient dephosphorization and degradation lecithin function pseudomonad (Pseudomonas sp.) MET69, China typical culture collection center has been preserved in it within the bacterial strain on September 12nd, 2016, deposit number is CCTCC NO:M2016474, bacterial strain MET69 of the present invention has efficient dephosphorization ability, when handling low concentration (P≤10mg/L) and high concentration (P about 20mg/L) phosphorus-containing wastewater respectively with the bacterium and with the chitosan crosslinked immobilized spherule being prepared into, it so that waste water is met " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) level-one B standard (P < 0.5mg/L), have a clear superiority compared with having reported bacterial strain；Research simultaneously it has also been found that, bacterial strain MET69 can degrade lecithin, provide excellent material for bio-bacterial manure and the preparation of phosphorus decomposing enzyme preparation, illustrate the bacterial strain phosphorus-containing wastewater administer and agricultural production in have broad application prospects.
Description
Technical field
The invention belongs to environmental microbiology fields, and in particular to the pseudomonad of one plant of efficient dephosphorization and degradation lecithin
And its wastewater treatment, in terms of application.
Background technique
In recent decades, with China human mortality increase severely, it is industrialized greatly develop, water eutrophication pollution problem is more
Seriously.It is generally believed that phosphorus is the main incitant of water eutrophication, waste water dephosphorization is prevent water eutrophication important
Approach；On the other hand, phosphorus is main nutrient elements needed for plant growth, and phosphorus element deficiency can seriously affect the growth of plant.For
Realize the sustainable development of phosphor resource, at present in waste water dephosphorization field, how to become traditional " processing " as " recycling ", by
Gradually object is paid close attention to as the research hotspot of countries in the world scholars and government.
Pseudomonas is extensive in distributed in nature, is one of the most common type Gram-negative bacteria present in soil,
In it is some have the functions such as antibacterial, nitrification and denitrification, be of great importance to biological control and environmental improvement.It is related at present false single
The report of born of the same parents bacterium is concentrated mainly on biological control and wastewater treatment etc..Such as patent 201510400702.3(China, Liu Lihe
Deng) Pseudomonas aeruginosa strain is reported, the leaf blight of corn, Causal Organism of Maize Basal Stalk, maize sheath blight, cabbage leaf can be effectively prevented
The plant diseases such as maize ear rot, asparagus bean rust, capsicum leaf rot；Patent 201410598044.9(China, Li Qiang etc.) have found one plant
The short distance nitration bacterium that ammonia nitrogen in sewage can be handled, is identified as pseudomonad, in sewage total nitrogen and COD have it is certain
Removal ability；Patent 200910032681.9(China, brightness Liu etc.) it discloses one plant and can solve lecithin, promote poplar seedlings growth
Bacillus cercus；In patent 201410665101.0(China, Zhu Xikun etc.) in mention the one plant of different phenol that can degrade
The pseudomonad of class compound has good application value in industrial wastewater of the processing containing phenol；Patent 201010538869.3
(China, Yuan Hongli etc.) then reports one plant of Pseudomonas stutzeri and its application in water body dephosphorized etc..It can currently, there is no
Processing phosphorus sewage has the pseudomonad report of degradation lecithin function again, and published phosphorus sewage water treatment method can not be realized
Recycling and reusing to phosphorus.
Bacterial strain MET69 of the present invention be a pseudomonas (Pseudomonas sp.), have both dephosphorization and lecithin of degrading
Function.After the bacterial strain has significant advantage compared with existing report in dephosphorization ability, and immobilized spherule is made in the bacterial strain, no
Only phosphor-removing effect is further promoted, and can also be realized recycling to phosphorus and be recycled.In addition, the phosphorus in soil has 20%
~50% is existing for the state of phytic acid, nucleoprotein and lecithin, it is difficult to is absorbed and used by plants, bacterial strain MET69 is to lecithin
Have degradation, soil can be significantly improved to the Phosphorus supply capacity of plant by being applied in soil, in preparation bio-bacterial manure and
There is certain application prospect in phosphorus decomposing enzyme preparation.Therefore, hair of the bacterial strain MET69 of the present invention to phosphorus wastewater treatment and biological husbantry
Exhibition all has significance.
Summary of the invention
The purpose of the present invention is to provide one plant of efficient dephosphorization and degradation lecithin pseudomonad (Pseudomonas sp.) MET69, and being fixed bead is applied to waste water dephosphorization.
Above-mentioned purpose that the invention is realized by the following technical scheme:
The pseudomonad of one plant of efficient dephosphorization and degradation lecithin, classification naming are as follows: pseudomonad (Pseudomonas sp.) MET69, it is preserved in China typical culture collection center, deposit number: CCTCC NO:M2016474, preservation day
Phase: on September 12nd, 2016.
The 16S rDNA sequence of bacterial strain MET69, is shown in sequence table.
The cultural method of above-mentioned pseudomonad MET69: beef-protein medium (10 g/L of peptone, beef extract 3
G/L, NaCl5 g/L, distilled water 1000ml, pH 7.0 ~ 7.2), 100 mL(250 mL conical flask of liquid amount), seed liquor inoculation
Measure the 1% of 1%(beef-protein medium volume), with 150 r/min in 28 DEG C of 10 h of culture.
The efficient dephosphorization of above-mentioned pseudomonad MET69 is studied: phosphorus-containing wastewater (PAM culture medium) is formulated (g/L): sodium citrate
4.0, sodium chloride 0.5, ammonium sulfate 2.5, calcium chloride 0.25, magnesium sulfate 0.25, potassium dihydrogen phosphate 0.0439, maltose 0.01, first
Aniline blue-O 0.025,1000ml, pH7.2,121 DEG C of sterilizing 30min of distilled water.
The immobilized spherule of above-mentioned pseudomonad MET69 handles the research of high phosphorus concentration waste water: simulated wastewater formula (g/
L): glucose 0.3, peptone 0.1, sodium acetate 0.15, sodium chloride 0.05, bitter salt 0.15, ammonium chloride 0.18, phosphorus
Acid dihydride potassium (in terms of P: 20:mg/L, 0.0878), 1000mL, pH7.0,115 DEG C of sterilizing 25min of distilled water.
The research that above-mentioned pseudomonad MET69 degrades to lecithin: basal medium: glucose 10.0 g, (NH4)2SO40.5 g, MgSO4·7H2O 0.3 g, NaCl0.3g, KCl0.3 g, FeSO4·7H2O 0.03 g, MnSO4·4H2O
0.03 g, 20 g of agar, distilled water 1000 mL, pH are naturally, 115 DEG C of sterilizing 25min.50~60 DEG C are cooled to after sterilizing.It will
It takes yolk to pour into sterilizing triangular flask after one raw egg surface sterilization, 50ml sterile water is added, mix and add by additive amount 10%
Enter in basal medium, obtains lecithin medium.
Compared with existing patented strain, bacterial strain of the present invention is had the advantage that
(1) with bacterial strain MET69 of the present invention processing low concentration wastewater (P≤10mg/L) interior for 24 hours that waste water can be made to meet " city when
Town Wastewater Treatment discharge standard " (GB18918-2002) level-one B standard (P < 0.5mg/L)；With bacterial strain of the present invention
When MET69 and chitosan crosslinked immobilized spherule handle high phosphorus concentration waste water (about 20 mg/L of P), 48h tp removal rate is reachable
98.54%, can meet after processing " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) level-one B standard (P <
0.5mg/L), illustrate bacterial strain MET69 except phosphate capacity is totally preferable, and good environmental adaptability, industrializing, upper application value is big.
(2) bacterial strain MET69 of the present invention to lecithin have preferable degradation capability, can with lecithin be unique phosphorus source
Culture medium on form significant phosphorus decomposing circle, MET69 microbial inoculum is imposed in soil, the rapid available phosphorus that can significantly improve soil contains
Amount, is good soil conditioner.
Detailed description of the invention
Fig. 1 is MET69 bacterial strain mostly except phosphate dyeing effect.
Fig. 2 is growth curve of the MET69 in beef extract-peptone fluid nutrient medium.
Fig. 3 is culture form of the MET69 bacterial strain on beef extract-peptone plate.
Fig. 4 is the 16S rDNA gene order phylogenetic tree of MET69 bacterial strain.
Fig. 5 is that the chitosan-immobilized bead of MET69 handles high phosphorus concentration waste water effect.
Fig. 6 is degradation effect of the MET69 to lecithin.
Specific embodiment
The following is specific embodiments of the present invention, is described further to technical solution of the present invention, but of the invention interior
Appearance is not limited solely to range described in embodiment, all to be included in this without departing substantially from the change of present inventive concept or equivalent substitute
Within the protection scope of invention.
The nutrient media components being related in case study on implementation:
PAM-TBO culture medium (g/L): sodium citrate 4.0, sodium chloride 0.5, ammonium sulfate 2.5, calcium chloride 0.25, magnesium sulfate
0.25, potassium dihydrogen phosphate 0.0439, maltose 0.01, toluidine blue-O 0.025, distilled water 1000ml, pH7.2, PAM-TBO
Culture medium is used for the qualitative screening of dephosphorization bacterial.
Rich phosphorus culture medium (g/L): sodium acetate 5, ammonium chloride 2, potassium dihydrogen phosphate 0.0439, bitter salt 0.5, chlorine
Change calcium 0.2, distilled water 1000ml, pH7.0 ~ 7.2, activation of the enriched medium for dephosphorization bacterial.
Beef-protein medium (g/L): peptone 10, beef extract 3, NaCl 5, distilled water 1000ml, pH7.0 ~
7.2, beef-protein medium is for the isolating and purifying of bacterial strain, preservation and domestication.
Simulated wastewater formula (g/L): glucose 0.3, peptone 0.1, sodium acetate 0.15, sodium chloride 0.05, seven hydration sulphur
Sour magnesium 0.15, ammonium chloride 0.18, potassium dihydrogen phosphate (in terms of P: 10mg/L, 0.0439；In terms of P: 20:mg/L, 0.0878), steam
Distilled water 1000mL, pH7.0.
Lecithin medium: basal medium (g/L): glucose 10.0, (NH4)2SO40.5, MgSO4·7H2O
0.3, NaCl 0.3, KCl 0.3, FeSO4·7H2O 0.03, MnSO4·4H2O 0.03, agar 20,1000 mL of distilled water
, pH is naturally, 115 DEG C of sterilizing 25min.50~60 DEG C are cooled to after basal medium sterilizing.After a raw egg surface sterilization
Take yolk to pour into sterilizing triangular flask, be added 50ml sterile water, mix by additive amount 10% be added basal medium in get
Lecithin medium.
1 pseudomonad of embodiment (Pseudomonas sp.) MET69 screening
VasviChaudhry and Chandra ShekharNautiyal is once 17 phases of volume 102 in 2011
A kind of method that high flux screening dephosphorization bacterial is reported on " Bioresource Technology ", can be right using toluidine blue
The characteristic for more removing phosphate granules stain in bacterial strain, can be with Rapid identification dephosphorization bacterial.Bacterial strain of the present invention is sieved by the method
Choosing obtains, concrete operation step:
1. collected Hubei Province Huangshi Yangxin County chemical plant wastewater sample and sterile water are mixed by the dilution proportion of 1:9
Even, gained suspension concentration is 10-1；
2. this 10 doubling dilution of bacteria suspension is respectively coated on rich phosphorus culture medium at various concentration again, 28 DEG C of inversion trainings
Support 48h；
3. the different bacterium colony of picking morphological feature carries out scribing line purifying, purifying bacterial strain is numbered, is retained spare；
4. above-mentioned bacterial strains are activated and obtain seed liquor, every hole adds 0.18ml liquid PAM-TBO to cultivate respectively on 96 orifice plates
Base, and 20 μ L bacterium solutions are inoculated with, the every three Kong Weiyi groups of file, i.e., each bacterial strain does 3 repetitions, is made with not being inoculated with the processing of bacterium sample
For control；
5. just setting 28 DEG C of 2 ~ 3d of culture with sealed membrane by 96 pore plate by sealing, discoloration in orifice plate, the result is shown in Figure 1 are observed.
Colour fading, which is more obvious, shows that bacterial strain dephosphorization ability is stronger, and to fading, apparent bacterial strain carries out the preservation of glycerol method.
By above-mentioned screening technique, 1 plant of bacterial strain that toluidine blue culture medium can be made significantly to fade is obtained, number is
MET69, i.e. this patent bacterial strain.It is seeded to after MET69 activation by 1% inoculum concentration (account for beef-protein medium volume 1%)
Beef extract-peptone fluid nutrient medium, growth curve are shown in Fig. 2.The logarithmic phase of MET69 is about in 10 ~ 28h as seen from the figure, at this time
MET69 activity is most strong, and dephosphorization efficiency is higher.
The identification of 2 bacterial strain MET69 of embodiment
(1) morphological feature and physiological and biochemical property
The form and physiological and biochemical property of bacterial strain MET69: 28 DEG C of constant temperature incubation 48h, bacterium on beef extract-peptone plate
, the smooth of the edge round in 2 ~ 3mm is fallen, in faint yellow, opaque, specific colonial morphology is shown in Fig. 3.Referring to " primary Jie Shi Bacteria Identification
Handbook ", it obtains the bacterial strain and also has the following characteristics that Gram-negative, thallus is rod-shaped, and no gemma and pod membrane are formed, amphimicrobian,
Atrichia, catalase positive, amylase reaction negative, methyl red is negative, V-P reaction negative.
(2) 16S rDNA Molecular Identification
The PCR amplification in the area 16S rDNA V3, primer sequence are as follows: 27F are carried out using bacterial strain DNA as template
(AGAGTTTGATCCTGGCTCAG) and 1492R(GGTTACCTTGTTACGACTT)；
PCR system establishes (50 μ L): 1 μ L of Template DNA, 1 μ L of Forward Primer (10uM), Reverse
1 μ L of Primer (10uM), 1 Taq-Plus μ L, 5 μ L of Taq-Plus Buffer, 5 μ L of dNTPs (2mM), 36 μ L of sterile water.
PCR response parameter are as follows: 95 DEG C of initial denaturations 5min, 94 DEG C of denaturation 30s, 55 DEG C of renaturation 45s, 72 DEG C of extension 90s, totally 30
A circulation, finally 72 DEG C of extension 10min again.
After PCR, 5 μ LPCR products is taken to carry out 0.8% agarose gel electrophoresis detection, target fragment size about 1.5kb.
It is sent after PCR product is recycled and holds up Kechuang neoformation Science and Technology Ltd. to Wuhan and be sequenced.Surveyed sequence length is 1566bp.
Measured sequence is subjected to BLAST comparison on NCBI, sequence and bacterial strain MET69 similitude are up to 99% as the result is shown
Be pseudomonas.The higher 16S rDNA sequence of correlation is downloaded, with the systematic growth of MEGA software building MET69
Tree, sees Fig. 4.
It is final to determine that bacterial strain MET69 is one in conjunction with strain morphology, physiological and biochemical property and 16S rDNA sequence alignment analysis
Pseudomonas (Pseudomonas sp.)。
The research of the bacterial strain phosphorus removal property of the present invention of embodiment 3
It in phosphorus concentration is respectively 5,10,15,20 mg/L to bacterial strain MET69 to evaluate the phosphorus removal property of bacterial strain of the present invention
Phosphorus-containing wastewater (PAM culture medium) in dephosphorizing rate be measured and compare.
By bacterial strain MET69 activation culture to OD600It is 1.0, turns by 10% inoculum concentration (for the volume 10% of PAM culture medium)
It is connected in the PAM culture medium (in terms of P: 5,10,15,20 mg/L) of 200 mL, is cultivated under the conditions of 28 DEG C, 150 r/min, every
12,24,36,48,60(h) sampling, after 10000 r/min are centrifuged 10 min, molybdenum antimony resistance colorimetric method measures the phosphorus concentration of supernatant
(GB 11893-89), and calculate dephosphorizing rate (the results are shown in Table 1).
Dephosphorizing rate (%)=(XSample introduction- XSample out)/XSample introduction× 100%
XSample introduction: the total phosphorus concentration of the enrichment culture liquid before access bacterial strain
XSample out: the total phosphorus concentration of the enrichment culture liquid after access bacterial strain
The different phosphorus concentration waste water effects of 1 MET69 of table processing compare
The result shows that bacterial strain energy efficient process low concentration (P≤10mg/L) phosphorus-containing wastewater of the present invention, after processing for 24 hours in can
Meet " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) level-one B standard (P < 0.5mg/L), phosphor-removing effect
Significantly, but when handling the phosphorus-containing wastewater of higher concentration (10 < P≤20mg/L), it is not able to satisfy discharge standard still.In order to improve bacterium
The phosphorus removal property and cycle performance of strain MET69, immobilizes treatment research to bacterial strain.
The 4 high phosphorus concentration waste water of bacterial strain MET69 immobilized spherule of embodiment (about 20 mg/L of P) processing
The preparation of immobilization blank bead: No. 8 syringe needles of 2.5% chitosan gum liquid solution are instilled into condensation water (20% methanol
And 30%NaOH) in, hollow globular chitosan ball is obtained after acting on 2 h, taking-up is washed with deionized, spare.
The preparation of immobilization embedded bacterium bead: the immobilization blank bead prepared is put into wet bacterium and sterile saline
Mass volume ratio (g:ml) be 2:100 bacteria suspension in, adsorb 2 h, take out chitosan bead simultaneously washed with sterile saline
It is spare afterwards.
2g immobilization blank bead, immobilization embedded bacterium bead are weighed respectively, are then respectively put into dense equipped with 200 ml phosphorus
Degree is to handle in 28 DEG C, 150 r/min shaking tables in 500 ml conical flasks of 20 mg/L waste water, at regular intervals 0,12,
24,36,48,72(h) 5 ml of waste water is taken, 10000 r/min are centrifuged 10 min in centrifuge, supernatant are taken, according to ammonium molybdate point
Light photometry (GB11893-89) measures wherein total phosphorus content, calculates dephosphorizing rate.
Experimental result is shown in attached drawings 5 to remove as shown, immobilization embedded bacterium bead handles high phosphorus concentration waste water in 48 h
Phosphorus effect reaches most preferably, dephosphorizing rate 98.54%, and the phosphorus concentration in system is 0.292 mg/L at this time.
The experimental results showed that handling high phosphorus concentration waste water (P about 20 with the crosslinking bead of pseudomonad MET69 and chitosan
Mg/L), it can be made to meet " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) level-one B in 48 h
Standard (P < 0.5mg/L).After illustrating that bacterial strain MET69 is immobilized, not only dephosphorization ability is further increased, and is also achieved to phosphorus element
Recycling and recycle.
Degradation of the 5 bacterial strain MET69 of embodiment to lecithin
MET69 verifies the degradation of lecithin using phosphorus decomposing circle method, by MET69 in beef extract-peptone liquid
After activating 10h in culture medium, after culture 48h is inverted at the flat lining out of lecithin (point sample), 37 DEG C, it is seen that the bacterium of MET69
Fall around occur apparent phosphorus decomposing circle (phosphorus decomposing circle refers on containing organic phosphorus solid medium, periphery of bacterial colonies generate because having
The transparent circle of the degradation of machine phosphorus and formation), the results are shown in attached figure 6.MET69 can with lecithin be unique phosphorus source plate on grow
And transparent phosphorus decomposing circle is formed, illustrate that MET69 has preferable degradation to lecithin.
Influence of 6 MET69 of embodiment to content of soil available phosphor
It takes the unmanured normal soil in university periphery as reference, verifies influence of the MET69 to soil quick-effective phosphor.Soil speed
Effect phosphorus is also referred to as soil available phosphorus, including water-soluble phosphorus and weak acid dissolubility phosphorus, rapid available phosphorus are easy to be absorbed and used by plants, therefore its content
It is an important indicator for judging soil Phosphorus supply capacity.The NaHCO of the present embodiment 0.5mol/L3(pH8.5) at digestion agent
Soil is managed, the phosphorus content of leachate is measured using molybdenum antimony resistance colorimetric method (GB 11893-89).
After MET69 is activated 10h in beef extract-peptone Liquid Culture, thallus and with sterile water wash 1 time is collected, then
With sterile water tune OD600To 1.0, bacteria concentration about 3 × 10 at this time8cfu/mL.The MET69 bacterium solution of every 100g soil sample addition 10mL is simultaneously
It stirs evenly, is placed under natural environment and in 0h, for 24 hours, 48h, 72h are separately sampled.
It weighs and is placed in 250ml triangular flask by the air-dried soil sample 5.00g of 1mm sieve pore, one small spoon of non-phosphorus active carbon is added
With the NaHCO of 0.5mol/L3(pH8.5) leaching liquor 100ml stoppers bottle stopper in acutely concussion 30min, uses drying after taking-up immediately
Funnel and the filtering of without phosphorus filter paper, filtrate another triangular flask access.10mL filtrate is taken to measure phosphorus concentration with molybdenum antimony resistance colorimetric method,
It substitutes into formula and calculates corresponding content of soil available phosphor, the results are shown in Table 2.
Content of soil available phosphor (mg/kg)=
Note:
Influence of 2 MET69 of table in 72h to soil available phosphorus content
As shown in Table 2, lower for trying the Phosphorus supply capacity of soil script, but after MET69 microbial inoculum is applied to soil 72h,
It is significantly improved for examination content of available phosphorus in soil, Phosphorus supply capacity is in medium level on the upper side, illustrates that MET69 is good soil improvement
Agent, can promote soil in time is plant supplement phosphorus element, and the application to chemical fertilizer is reduced reduces agricultural cost, realizes to soil
The biological prosthetic of earth environment is of great significance.
Sequence table
<110>Hubei University
The pseudomonad of<120>one plants of efficient dephosphorizations and degradation lecithin
<160>3
<210>1
<211>1566
<212>DNA
<213>pseudomonad (Pseudomonas sp.)
<400>1
ggcctgcagg tcgacgattg gttaccttgt tacgacttca ccccagtcat gaatcactcc 60
gtggtaaccg tcccccttgc ggttagacta gctacttctg gagcaaccca ctcccatggt 120
gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc gtgacattct gattcacgat 180
tactagcgat tccgacttca cgcagtcgag ttgcagactg cgatccggac tacgatcggt 240
tttatgggat tagctccacc tcgcggcttg gcaacccttt gtaccgacca ttgtagcacg 300
tgtgtagccc tggccgtaag ggccatgatg acttgacgtc atccccacct tcctccggtt 360
tgtcaccggc agtctcctta gagtgcccac ccgaggtgct ggtaactaag gacaagggtt 420
gcgctcgtta cgggacttaa cccaacatct cacgacacga gctgacgaca gccatgcagc 480
acctgtgtct gagttcccga aggcaccaat ccatctctgg aaagttctca gcatgtcaag 540
gccaggtaag gttcttcgcg ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg 600
cccccgtcaa ttcatttgag ttttaacctt gcggccgtac tccccaggcg gtcgacttat 660
cgcgttagct gcgccactaa gatctcaagg atcccaacgg ctagtcgaca tcgtttacgg 720
cgtggactac cagggtatct aatcctgttt gctccccacg ctttcgcacc tcagtgtcag 780
tatcagtcca ggtggtcgcc ttcgccactg gtgttccttc ctatatctac gcatttcacc 840
gctacacagg aaattccacc accctctacc gtactctagc tcagtagttt tggatgcagt 900
tcccaggttg agcccgggga tttcacatcc aacttgctga accacctacg cgcgctttac 960
gcccagtaat tccgattaac gcttgcaccc ttcgtattac cgcggctgct ggcacgaagt 1020
tagccggtgc ttattctgtt ggtaacgtca aaacagcaag gtattaactt actgcccttc 1080
ctcccaactt aaagtgcttt acaatccgaa gaccttcttc acacacgcgg catggctgga 1140
tcaggccttc gcccattgtc caatattctc cactgctgcc tcccgtagga gtctggaccg 1200
tgtctcagtt ccagtgtgac tgatcatcct ctcagaccag ttacggatcg tcgccttggt 1260
aggcctttac cccaccaact agctaatccg acctaggctc atctgatagc gtgaggtccg 1320
aagatccccc actttctccc tcaggacgta tgcggtatta gcgcccgttt ccggacgtta 1380
tcccccacta ccaggcagat tcctaggcat tactcacccg tccgccgctg aatccaggag 1440
caagctccct tcatccgctc gacttgcatg tgttaggcct gccgccagcg ttcaatctga 1500
gccaggatca aactctaatc tctagaggat ccccgggtac cgagctcgaa ttcgtaatca 1560
gttttc 1566
<210> 2
<211> 20
<212> DNA
<213>artificial sequence
<400> 2
agagtttgat cctggctcag 20
<210> 3
<211> 19
<212> DNA
<213>artificial sequence
<400> 3
ggttaccttg ttacgactt 19
Claims (1)
1. the pseudomonad of one plant of efficient dephosphorization and degradation lecithin, it is characterised in that: the pseudomonad is Pseudomonas
Sp.MET69, has been preserved in China typical culture collection center on September 12nd, 2016, and deposit number is CCTCC NO:M
2016474；The pseudomonad Pseudomonas sp.MET69 and its immobilized spherule can handle phosphorus-containing wastewater, the pseudomonad
Pseudomonas sp.MET69 has degradation to lecithin, can also be applied to the preparation of bio-bacterial manure and phosphorus decomposing enzyme preparation.
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| CN108546658B (en) * | 2018-04-10 | 2020-09-04 | 暨南大学 | Phosphorus-dissolving bacterium and compound bacterium agent of phosphorus-dissolving bacterium and DEHP degrading bacterium and application of phosphorus-dissolving bacterium and compound bacterium agent |
| CN108795799B (en) * | 2018-05-31 | 2019-12-03 | 山东省科学院生态研究所 | One plant for controlling multi-functional arthrobacterium Fp64 and its application of phosphorus levels |
| CN116891809B (en) * | 2022-12-06 | 2023-12-19 | 浙江大学 | Pseudomonas asiatica and microbial agent and application thereof |
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