CN101698881A - Lecithase test medium - Google Patents
Lecithase test medium Download PDFInfo
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- CN101698881A CN101698881A CN200710115731A CN200710115731A CN101698881A CN 101698881 A CN101698881 A CN 101698881A CN 200710115731 A CN200710115731 A CN 200710115731A CN 200710115731 A CN200710115731 A CN 200710115731A CN 101698881 A CN101698881 A CN 101698881A
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- lecithase
- test medium
- yolk
- peptone
- glucose
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Abstract
The invention relates to a lecithase test medium, which comprises the main components of peptone, sodium chloride, disodium hydrogen phosphate, magnesium sulfate, glucose, agar, 50 percent egg yolk normal saline emulsion and distilled water, and is prepared through the processes of mixing the components in proportion, heating, dissolving, mixing, cooling and sub-packaging the mixture, performing high-temperature sterilization and the like. The lecithase test medium is mainly used for checking the activity of bacterial lecithase. The key problem of a biological experiment is to grasp the specific ecological action of organisms. For evaluating the correctness and the specificity of an experimental method and checking the activity of the bacterial lecithase, the acting time is necessary to be grasped strictly to obtain the accurate characteristics of microorganisms to perform detection and diagnosis quickly, so the method is required to be simple and has convenient use and reliable results. The lecithase test medium can be used for quickly detecting and diagnosing pseudomonas fluorescens. The reaction can be influenced by the pH value, the types of the peptone and the like; a calcium salt can inhibit the reaction, and 1 percent glucose is added into the culture medium to obtain a good result. A beef heart muscle immersion can be prepared into a liquid medium by adding an equal amount of 10 percent yolk saline suspension, and inoculation bacteria to be tested are cultivated for 1 to 5 days to show significant positive turbidity.
Description
Technical field the present invention relates to a kind of Lecithase test medium, its main component be peptone, sodium-chlor, Sodium phosphate dibasic, sal epsom glucose, agar, 50% yolk physiological saline emulsion, distilled water be mixed in proportion through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of Lecithase test medium.Be mainly used in the activity of checking the bacterium lecithinase.Key problem of biotic experiments is to grasp characteristic ecology action of organisms.The accuracy of evaluation experimental method and specificity, the activity of inspection bacterium lecithinase must strictly be grasped action time, obtains microorganism characteristic accurately, the diagnosis of ability rapid detection, this just requires method simple, and is easy to use, reliable results.Lecithase test medium can be used for the rapid detection diagnosing fluorescent pseudomonas.The existing base kind of cultivating is a lot, does not have solid method; Substratum itself or with the product of bacterial reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to Study on culture medium also in continuous development.The Bioexperiment worker wishes to have always and a kind ofly can overcome the insufficient Lecithase test medium of above-mentioned substratum.This reaction can be influenced by pH value, peptone kind etc., and calcium salt can have restraining effect to reaction, adds 1% glucose and can obtain good result in substratum.Available bovine cardiac immersion liquid adds equivalent 10% yolk salt aqueous suspensions and makes liquid nutrient medium, and inoculation is waited to try bacterium and cultivated 1～5 day, and it is positive to present remarkable muddiness.
Background technology the object of the present invention is to provide that a kind of energy is a kind of to overcome the insufficient Lecithase test medium of above-mentioned substratum.This reaction can be influenced by pH value, peptone kind etc., and calcium salt can have restraining effect to reaction, adds 1% glucose and can obtain good result in substratum.Available bovine cardiac immersion liquid adds equivalent 10% yolk salt aqueous suspensions and makes liquid nutrient medium, and inoculation is waited to try bacterium and cultivated 1～5 day, and it is positive to present remarkable muddiness.Compare with other class neutralizing agent and to possess following characteristics: Lecithase test medium can be used for the rapid detection diagnosing fluorescent pseudomonas. This reaction can be influenced by pH value, peptone kind etc., and calcium salt can have restraining effect to reaction, adds 1% glucose and can obtain good result in substratum.Available bovine cardiac immersion liquid adds equivalent 10% yolk salt aqueous suspensions and makes liquid nutrient medium, and inoculation is waited to try bacterium and cultivated 1～5 day, and it is positive to present remarkable muddiness.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, mixing, cooling, high-temperature sterilization, packing, etc. technology form a kind of Lecithase test medium.
Prescription following (component and weight percent % thereof) the peptone 18-20 that the present invention selects; Sodium-chlor 0.8-1.0; Sodium phosphate dibasic 2.0-2.5; Sal epsom 0.05-0.07; Glucose 0.8-1.0; Agar 10-12; 50% yolk physiological water emulsion adds to 100; (distilled water diluting is to 1000mL).
Lecithinase belongs to the phosphatide hydrolase, the lecithinase that clostridium perfringens produces, the lethal toxin that A type bacterium is main, under the condition that calcium, magnesium ion exist, hydrolyzed lecithin generates diglyceride and phosphorylcholine rapidly, is mainly used in to check the bacterium lecithin activity.Producing muddy ring around the culture is glyceryl ester, and yolk is decomposed the free-fat that produces and vitellin reaction and produces precipitation.To wait to try bacterium 18～24h culture and be inoculated on flat board or the slant medium, cultivate 18～24h observations for 36 ℃.Produce the positive reaction of the muddy ring of oyster white in periphery of bacterial colonies.Quality Control positive strain: Pseudomonas fluorescens.Negative strain: Pseudomonas aeruginosa.This reaction can be influenced by pH value, peptone kind etc., and calcium salt can have restraining effect to reaction, adds 1% glucose and can obtain good result in substratum.Available bovine cardiac immersion liquid adds equivalent 10% yolk salt aqueous suspensions and makes liquid nutrient medium, and inoculation is waited to try bacterium and cultivated 1～5 day, and it is positive to present remarkable muddiness.
The preparation method of product of the present invention is:
(1), except that yolk liquid, the dissolving of other composition Hybrid Heating is transferred pH7.4,115 ℃ of 15min autoclavings.Be chilled to about 50 ℃,
(2), add aseptic yolk liquid, mix, pour into the every pipe 10mL of aseptic plate or packing bevel.
The present invention has the following advantages: Lecithase test medium can be used for the rapid detection diagnosing fluorescent pseudomonas. This reaction can be influenced by pH value, peptone kind etc., and calcium salt can have restraining effect to reaction, adds 1% glucose and can obtain good result in substratum.Available bovine cardiac immersion liquid adds equivalent 10% yolk salt aqueous suspensions and makes liquid nutrient medium, and inoculation is waited to try bacterium and cultivated 1～5 day, and it is positive to present remarkable muddiness.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates to a kind of Lecithase test medium, its main component be peptone, sodium-chlor, Sodium phosphate dibasic, sal epsom glucose, agar, 50% yolk physiological saline emulsion, distilled water be mixed in proportion through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of Lecithase test medium; It is characterized in that having following prescription (component and weight percent % thereof): peptone 18-20; Sodium-chlor 0.8-1.0; Sodium phosphate dibasic 2.0-2.5; Sal epsom 0.05-0.07; Glucose 0.8-1.0; Agar 10-12; 50% yolk physiological water emulsion adds to 100; (distilled water diluting is to 1000mL).
2. the preparation method of the described Lecithase test medium of claim 1 is characterized in that having following step:
(1), except that yolk liquid, the dissolving of other composition Hybrid Heating is transferred pH7.4,115 ℃ of 15min autoclavings.Be chilled to 50 ℃;
(2), add aseptic yolk liquid, mix, pour into the every pipe 10mL of aseptic plate or packing bevel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115731A CN101698881A (en) | 2007-12-18 | 2007-12-18 | Lecithase test medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115731A CN101698881A (en) | 2007-12-18 | 2007-12-18 | Lecithase test medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698881A true CN101698881A (en) | 2010-04-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115731A Pending CN101698881A (en) | 2007-12-18 | 2007-12-18 | Lecithase test medium |
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| CN (1) | CN101698881A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244502A (en) * | 2016-09-27 | 2016-12-21 | 湖北大学 | One strain efficient dephosphorization and the pseudomonas of degraded lecithin |
-
2007
- 2007-12-18 CN CN200710115731A patent/CN101698881A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244502A (en) * | 2016-09-27 | 2016-12-21 | 湖北大学 | One strain efficient dephosphorization and the pseudomonas of degraded lecithin |
| CN106244502B (en) * | 2016-09-27 | 2019-06-18 | 湖北大学 | The pseudomonad of one plant of efficient dephosphorization and degradation lecithin |
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Open date: 20100428 |