CN106244459A - A kind of pseudomonas dry powder and preparation method thereof - Google Patents
A kind of pseudomonas dry powder and preparation method thereof Download PDFInfo
- Publication number
- CN106244459A CN106244459A CN201610783399.4A CN201610783399A CN106244459A CN 106244459 A CN106244459 A CN 106244459A CN 201610783399 A CN201610783399 A CN 201610783399A CN 106244459 A CN106244459 A CN 106244459A
- Authority
- CN
- China
- Prior art keywords
- pseudomonas
- dry powder
- protective agent
- powder
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of pseudomonas dry powder and preparation method thereof.Pseudomonas dry powder disclosed by the invention includes that pseudomonas and protective agent, described protective agent include defatted milk powder, mannitol, sodium glutamate or glycine, corn starch, trehalose.The pseudomonas viable bacteria yield that the present invention provides is up to 70%, and compared with prior art, the pseudomonas mycopowder Viable detection of the present invention is high.
Description
Technical field
The present invention relates to belong to antibacterial lyophilisation Techniques of preserving field, particularly to a kind of pseudomonas dry powder and
Its preparation method.
Background technology
Gram negative bacteria to temperature, pH, nutrient environment adaptability to changes relatively strong, there is fast growth, environment adapts to
The advantages such as ability is strong.Pseudomonas belongs to gram negative bacteria, is one of microorganism that distributed in nature is the widest, the most surely grows in soil
In earth, water and root system of plant, its biodegradation is strong with biotransformation capacity, therefore at environment remediation, Biological control, bioconversion
Etc. aspect have broad application prospects.But, gram negative bacteria, without spore hypopus, to extreme environment (hydropenia, hunger, height
Temperature, low temperature) resistivity poor, be dried and preservation survival rate low.
The application prospect wide due to pseudomonas and outstanding contaminant degradation effect, therefore, imitate contaminant degradation
Outstanding bacterial strain preserves for a long time and is necessary.
Vacuum Freezing & Drying Technology is widely used in terms of microbial preservation, is the widest bacterium of current adaptability
Agent technology of preparing, under low temperature, vacuum condition, the physiological activity of microorganism stops, and cell viability is difficult to impaired, is particularly suitable for heat
Quick property, the microorganism of oxygen-sensitive are dried in preparation, and microbial inoculum preservation and are susceptible to pollute, it is easy to subpackage transports.But, due to all kinds of
There is larger difference in the habitat conditions of microbial strains, nutritional need, cultural method etc., causes freezing of different microorganisms bacterial strain
Dry protective agent and freeze drying process are also not quite similar.
Summary of the invention
It is contemplated that overcome the defect of prior art, solve the problem of the frozen-dried protective of pseudomonas in prior art,
A kind of pseudomonas dry powder and preparation method thereof is provided.The present invention provides a kind of Rhodopseudomonas nontoxic, safe, easy to use
The freeze drying protectant of antibacterial and freeze drying process, preserve for vacuum lyophilization, it is possible to realize pseudomonas length at normal temperatures
Phase preservation also preserves vigor, provides technical support for carrying out the correlational study of pseudomonas strain in a deep going way.
For achieving the above object, the present invention is by the following technical solutions:
On the one hand, the present invention provides a kind of pseudomonas dry powder, and including pseudomonas and protective agent, described protective agent includes
Defatted milk powder, mannitol, sodium glutamate or glycine, corn starch, trehalose.Trehalose is in freezing process and dehydration
It is provided that the effect of being effectively protected, effectively prevents active component generation degeneration；Mannitol prevents active component with steam
Distillation loss, and make active component shape；Owing to aminoacid ion has acid, alkali both sexes, therefore, it is possible to low at biological product
Temperature preserves and the pH value of biological product can be adjusted by the pH change of energy suppression solution in freezing dry process, sodium glutamate or glycine
The whole stability region to active substance, thus reach to protect the purpose of active component.Defatted milk powder, in outside, makes solution
In supercooled state, solute concentration can be reduced at a certain temperature, thus play a protective role.
Preferably, in the pseudomonas dry powder that the present invention provides, described protective agent also includes yeast powder and/or Span 60.
Yeast powder, Span 60 improve mycopowder viable bacteria yield, and wherein, Span 60 is beneficial to improve the shelf life of pseudomonas dry powder mycopowder.
Preferably, in the pseudomonas dry powder that the present invention provides, (can on the basis of the gross weight of described pseudomonas dry powder
On the basis of the gross weight of viable bacteria suspension described below), including: 5wt%～25wt% defatted milk powder, 0.1wt%～
10wt% mannitol, 1wt%～15wt% sodium glutamate or glycine, 0.1wt%～3wt% corn starch, 1wt%～
10wt% trehalose, 0～15wt% yeast powder, 0-2wt% Span 60.
Preferably, in the pseudomonas dry powder that the present invention provides, (can on the basis of the gross weight of described pseudomonas dry powder
On the basis of the gross weight of viable bacteria suspension described below), including: 8wt%～20wt% defatted milk powder, 2wt%～8wt% are sweet
Dew alcohol, 2wt%～12wt% sodium glutamate or glycine, 0.5wt%～2.5wt% corn starch, 2wt%～8wt% Sargassum
Sugar, 1～12wt% yeast powder, 0.1-1.5wt% Span 60.
On the other hand, present invention also offers the preparation method of a kind of pseudomonas dry powder, including step: S1, by vacation list
Born of the same parents' fermented liquid is centrifuged, and abandons supernatant, obtains the first bacterium mud；S2, use brine the first bacterium mud, centrifugal, abandon supernatant, obtain second
Bacterium mud；S3, with sterilized water, above-mentioned second bacterium mud is configured to viable bacteria suspension；S4: by above-mentioned viable bacteria suspension and claim 1-4
After protective agent mixing in protective agent pseudomonas preparation described in any one, freezing, place into the vacuum lyophilization of pre-cooling
Lyophilizing in machine, obtains pseudomonas dry powder.
Preferably, in described step S1: rotating speed is 2000～10000rpm, centrifugal treating 5～30min.
Preferably, in described step S2: rotating speed is 2000～10000rpm, centrifugal treating 5～30min.
Preferably, described step S3 includes: with sterilized water, above-mentioned second bacterium mud is configured to 1.0 × 109Cfu/g～1.0
×1011Cfu/g viable bacteria suspension.
Preferably, described step S4 includes: by above-mentioned viable bacteria suspension and the guarantor in the pseudomonas preparation of present invention offer
Protect agent to be sufficiently mixed after uniformly, quick freezing 3～4h, place into lyophilizing in the vacuum freeze drier lyophilizing cabin of pre-cooling, obtain
Pseudomonas dry powder.Such as, described step S4 includes: in the pseudomonas preparation provide above-mentioned viable bacteria suspension and the present invention
After protective agent is sufficiently mixed uniformly, quick freezing 3～4h under the conditions of being first placed in-80 DEG C, place into the vacuum lyophilization of pre-cooling
In machine lyophilizing cabin, under the conditions of vacuum 0.1mbar ,-83 DEG C of lyophilizing cabin after lyophilizing 48h, obtain pseudomonas dry powder.
The beneficial effects of the present invention is: the pseudomonas viable bacteria yield that the present invention provides is up to 70%, with prior art
Comparing, the pseudomonas mycopowder Viable detection of the present invention is high.
The present inventor finds through substantial amounts of experiment: treat that the pseudomonas of vacuum lyophilization is by adding this
After the protective agent of bright offer processes, it is possible to increase pseudomonas, to dry, the protective capacities of low temperature, effectively reduces pseudomonas and exists
Loss of activity during vacuum lyophilization, and in recovery process, accelerate pseudomonas recovery.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, and not structure
Become limitation of the present invention.
In embodiment of the present invention 1-4, preparation pseudomonas culture medium: yeast powder 5g/L, tryptone 10g/L, sodium chloride
10g/L。
Table 1 below is the component of protective agent P1-P4 used in embodiment 1-4 and content (with protectant gross weight is
Benchmark) (content wt%):
Table 1
The calculating of embodiment of the present invention 1-4 strain survival rate is carried out in the following way: pseudomonas dry powder D1-D4 is multiple
Water, water-bath 30-60min at 30～35 DEG C, serial dilution counting after mixing, calculating protective agent, for the survival rate of strain, i.e. freezes
The ratio of same volume viable count before and after Gan.
Embodiment 1
1, cultivate under normal conditions and respectively treat preservation or cryodesiccated bacterial strain, the Rhodopseudomonas list bacterium of picking pure culture
Fall to being inoculated in LB fluid medium, be placed in biochemical cultivation case 30 DEG C, cultivate 12h under the conditions of 150rpm after, by vacation unit cell
Fermented liquid is centrifuged (rotating speed is 2000rpm, centrifugal treating 30min), abandons supernatant, obtains the first bacterium mud.
2, with brine the first bacterium mud, centrifugal (rotating speed is 1800rpm, centrifugal treating 25min), abandon supernatant,
Second bacterium mud.
3, with sterilized water, above-mentioned second bacterium mud is configured to 1.0 × 109Cfu/g viable bacteria suspension.
4, above-mentioned viable bacteria suspension is mixed homogeneously with protective agent P1, stand-by.Matched group 1 (without protective agent P1) is set.
Vacuum lyophilization condition is: quick freezing 3～4h under the conditions of being first placed in-80 DEG C, places into the vacuum freeze drier of pre-cooling
In lyophilizing cabin, under the conditions of vacuum 0.1mbar ,-83 DEG C of lyophilizing cabin after lyophilizing 48h, obtain pseudomonas dry powder D1.
Viable count by colony counting method detection pseudomonas dry powder D1.The pseudomonas vacuum freezing of the present embodiment 1
Drying protectant P1 is as shown in table 2 below to the protected effect of pseudomonas:
Table 2
Adding the pseudomonas vacuum lyophilization protective agent P1 of the present embodiment 1, gained dry powder viable count is 1.18 ×
109Cfu/g, it is 3.74 × 10 that matched group 1 is not added with protectant viable count8cfu/g.Result shows that adding protective agent P1 can show
The viable count of pseudomonas mycopowder after work raising vacuum lyophilization.
Using defatted milk powder 10wt%, sodium glutamate 5wt%, trehalose 5wt% is that freeze drying protectant prepares mycopowder, 37 DEG C
20d survival rate is 38%, and the 37 DEG C of 20d survival rates of dry powder using the protective agent P1 in embodiment 1 to prepare are 70%, and matched group is done
37 DEG C of 20d survival rates of powder are 20%.
Embodiment 2
1, cultivate under normal conditions and respectively treat preservation or cryodesiccated bacterial strain, the Rhodopseudomonas list bacterium of picking pure culture
Fall to being inoculated in LB fluid medium, be placed in biochemical cultivation case 30 DEG C, cultivate 12h under the conditions of 150rpm after, by vacation unit cell
Fermented liquid is centrifuged (rotating speed is 10000rpm, centrifugal treating 5min), abandons supernatant, obtains the first bacterium mud.
2, with brine the first bacterium mud, centrifugal (rotating speed is 5000rpm, centrifugal treating 20min), abandon supernatant,
Second bacterium mud.
3, with sterilized water, above-mentioned second bacterium mud is configured to 1.0 × 1011Cfu/g viable bacteria suspension.
4, above-mentioned viable bacteria suspension is mixed homogeneously with protective agent P2, stand-by.Matched group 2 (without protective agent P2) is set.
Vacuum lyophilization condition is: quick freezing 3.5h under the conditions of-80 DEG C, places into the vacuum freeze drier lyophilizing cabin of pre-cooling
In, under the conditions of vacuum 0.2mbar ,-85 DEG C of lyophilizing cabin after lyophilizing 43h, obtain pseudomonas dry powder D2.
Viable count by colony counting method detection pseudomonas dry powder D2.The pseudomonas vacuum freezing of the present embodiment 2
Drying protectant P2 is as shown in table 3 below to the protected effect of pseudomonas:
Table 3
Adding the pseudomonas vacuum lyophilization protective agent P2 of the present embodiment 2, gained dry powder viable count is 8.70 ×
1010Cfu/g, it is 3.27 × 10 that matched group 2 is not added with protectant viable count10cfu/g.Result shows to add protective agent P2 can
Significantly improve the viable count of pseudomonas mycopowder D2 after vacuum lyophilization.
Using defatted milk powder 5wt%, glycine 5wt%, trehalose 6wt% is that freeze drying protectant prepares mycopowder, 37 DEG C of 20d
Survival rate is 36%, and the 37 DEG C of 20d survival rates of dry powder using the protective agent P2 in embodiment 1 to prepare are 75%, matched group dry powder
37 DEG C of 20d survival rates are 20%.
Embodiment 3
1, cultivate under normal conditions and respectively treat preservation or cryodesiccated bacterial strain, the Rhodopseudomonas list bacterium of picking pure culture
Fall to being inoculated in LB fluid medium, be placed in biochemical cultivation case 30 DEG C, cultivate 12h under the conditions of 150rpm after, by vacation unit cell
Fermented liquid is centrifuged (rotating speed is 6000rpm, centrifugal treating 20min), abandons supernatant, obtains the first bacterium mud.
2, with brine the first bacterium mud, centrifugal (rotating speed is 10000rpm, centrifugal treating 5min), abandon supernatant,
Second bacterium mud.
3, with sterilized water, above-mentioned second bacterium mud is configured to 1.0 × 1010Cfu/g viable bacteria suspension.
4, above-mentioned viable bacteria suspension is mixed homogeneously with protective agent P3, stand-by.Matched group 3 (without protective agent P3) is set.
Vacuum lyophilization condition is: quick freezing 4h under the conditions of-78 DEG C, places in the vacuum freeze drier lyophilizing cabin of pre-cooling,
Under the conditions of vacuum 0.1mbar ,-83 DEG C of lyophilizing cabin after lyophilizing 48h, obtain pseudomonas dry powder D3.
Viable count is detected by colony counting method.The pseudomonas vacuum lyophilization protective agent P3 of the present embodiment 3 is to vacation
The protected effect of Zymomonas mobilis is as shown in table 4 below:
Table 4
Adding the pseudomonas vacuum lyophilization protective agent P3 of the present embodiment 3, gained dry powder viable count is 1.75 ×
1010Cfu/g, being not added with protectant viable count is 4.21 × 109cfu/g.Result shows that adding protective agent can significantly improve very
The viable count of pseudomonas mycopowder after vacuum freecing-dry.
Using defatted milk powder 5wt%, sodium glutamate 3wt%, trehalose 10wt% is that freeze drying protectant prepares mycopowder, 37 DEG C
20d survival rate is 42%, and the 37 DEG C of 20d survivals of dry powder using the protective agent P3 of the present embodiment 3 to prepare are 78%, matched group dry powder
37 DEG C of 20d survival rates are 20%.
Embodiment 4
1, cultivate under normal conditions and respectively treat preservation or cryodesiccated bacterial strain, by centrifugal for pseudomonas fermentation liquid (rotating speed
For 11000rpm, centrifugal treating 7min), abandon supernatant, obtain the first bacterium mud；
2, with brine the first bacterium mud, centrifugal (rotating speed is 2000rpm, centrifugal treating 35min), abandon supernatant,
Second bacterium mud；
3, with sterilized water, above-mentioned second bacterium mud is configured to 1.0 × 108Cfu/g viable bacteria suspension；
4, above-mentioned viable bacteria suspension is mixed homogeneously with protective agent P4, stand-by.Matched group 4 (without protective agent P4) is set.
Vacuum lyophilization condition is: quick freezing 3h under the conditions of-85 DEG C, places in the vacuum freeze drier lyophilizing cabin of pre-cooling,
Under the conditions of vacuum 0.1mbar ,-83 DEG C of lyophilizing cabin after lyophilizing 46h, obtain pseudomonas dry powder D4.
Viable count is detected by colony counting method.The pseudomonas vacuum lyophilization protective agent P4 of the present embodiment 4 is to vacation
The protected effect of Zymomonas mobilis is as shown in table 5 below:
Table 5
Adding the pseudomonas vacuum lyophilization protective agent of the present invention, gained dry powder viable count is 1.35 × 108cfu/
G, being not added with protectant viable count is 4.67 × 107cfu/g.Result shows that interpolation protective agent can significantly improve vacuum freezing and do
The viable count of dry rear pseudomonas mycopowder.
Using defatted milk powder 5wt%, sodium glutamate 3wt%, trehalose 10wt% is that freeze drying protectant prepares mycopowder, 37 DEG C
20d survival rate is 42%, and the 37 DEG C of 20d survivals of dry powder using the protective agent P4 of the present embodiment 4 to prepare are 78%, and matched group 4 is dry
37 DEG C of 20d survival rates of powder are 20%.
By table 2-table 5 it can be seen that the viable bacteria rate of the pseudomonas mycopowder of the present invention is high, and have the most stable
Property, it is being dried and storing process is being stablized.
The detailed description of the invention of present invention described above, is not intended that limiting the scope of the present invention.Any basis
Various other done by the technology design of the present invention change and deformation accordingly, should be included in the guarantor of the claims in the present invention
In the range of protecting.
Claims (9)
1. a pseudomonas dry powder, including pseudomonas and protective agent, described protective agent includes defatted milk powder, mannitol, paddy
Propylhomoserin sodium or glycine, corn starch, trehalose.
2. pseudomonas dry powder as claimed in claim 1, it is characterised in that described protective agent also includes yeast powder and/or department
Class 60.
3. pseudomonas dry powder as claimed in claim 1 or 2, it is characterised in that with the gross weight of described pseudomonas dry powder
On the basis of, including: 5wt%～25wt% defatted milk powder, 0.1wt%～10wt% mannitol, 1wt%～15wt% sodium glutamate
Or glycine, 0.1wt%～3wt% corn starch, 1wt%～10wt% trehalose, 0～15wt% yeast powder, 0-2wt% department
Class 60.
4. pseudomonas dry powder as claimed in claim 3, it is characterised in that with the gross weight of described pseudomonas dry powder as base
Standard, including: 8wt%～20wt% defatted milk powder, 2wt%～8wt% mannitol, 2wt%～12wt% sodium glutamate or sweet ammonia
Acid, 0.5wt%～2.5wt% corn starch, 2wt%～8wt% trehalose, 1～12wt% yeast powder, 0.1-1.5wt% department
Class 60.
5. the preparation method of the pseudomonas dry powder according to any one of claim 1-4, it is characterised in that include walking as follows
Rapid: S1, pseudomonas fermentation liquid to be centrifuged, abandon supernatant, obtain the first bacterium mud；S2, use brine the first bacterium mud, centrifugal,
Abandon supernatant, obtain the second bacterium mud；S3, with sterilized water, above-mentioned second bacterium mud is configured to viable bacteria suspension；S4: by above-mentioned viable bacteria suspension with
After protective agent mixing in protective agent pseudomonas preparation according to any one of claim 1-4, freezing, place into pre-cooling
Lyophilizing in vacuum freeze drier, obtains pseudomonas dry powder.
6. the preparation method of pseudomonas dry powder as claimed in claim 5, it is characterised in that in described step S1: rotating speed is
2000～10000rpm, centrifugal treating 5～30min.
7. the preparation method of pseudomonas dry powder as claimed in claim 5, it is characterised in that in described step S2: rotating speed is
2000～10000rpm, centrifugal treating 5～30min.
8. the preparation method of pseudomonas dry powder as claimed in claim 5, it is characterised in that described step S3 includes: use nothing
Above-mentioned second bacterium mud is configured to 1.0 × 10 by bacterium water9Cfu/g～1.0 × 1011The viable bacteria suspension of cfu/g.
9. the preparation method of pseudomonas dry powder as claimed in claim 5, it is characterised in that described step S4 includes: by upper
State after viable bacteria suspension is sufficiently mixed uniformly with the protective agent in pseudomonas dry powder according to any one of claim 1-4, soon
Quickly cooling freezes 3～4h, places in the vacuum freeze drier lyophilizing cabin of pre-cooling after lyophilizing, obtains pseudomonas dry powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610783399.4A CN106244459A (en) | 2016-08-31 | 2016-08-31 | A kind of pseudomonas dry powder and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610783399.4A CN106244459A (en) | 2016-08-31 | 2016-08-31 | A kind of pseudomonas dry powder and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106244459A true CN106244459A (en) | 2016-12-21 |
Family
ID=58079972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610783399.4A Pending CN106244459A (en) | 2016-08-31 | 2016-08-31 | A kind of pseudomonas dry powder and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106244459A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI737210B (en) * | 2020-03-06 | 2021-08-21 | 財團法人食品工業發展研究所 | Starter culture protection formula and use thereof |
| CN115851444A (en) * | 2023-03-01 | 2023-03-28 | 北京民海生物科技有限公司 | Freeze-drying protective agent for pneumococcus preservation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974607A (en) * | 2010-10-26 | 2011-02-16 | 冯广青 | Reagent and kit for detecting sensitivity of culture medium |
| CN104403982A (en) * | 2014-12-18 | 2015-03-11 | 南京工业大学 | Preparation method of oil displacement microbe high-density concentrated microbial inoculant by fermentation and freeze-drying |
| CN104962549A (en) * | 2015-07-27 | 2015-10-07 | 厦门市科环海洋生物科技有限公司 | Rhodopseudomonas palustris synchronized concentration method and application |
-
2016
- 2016-08-31 CN CN201610783399.4A patent/CN106244459A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974607A (en) * | 2010-10-26 | 2011-02-16 | 冯广青 | Reagent and kit for detecting sensitivity of culture medium |
| CN104403982A (en) * | 2014-12-18 | 2015-03-11 | 南京工业大学 | Preparation method of oil displacement microbe high-density concentrated microbial inoculant by fermentation and freeze-drying |
| CN104962549A (en) * | 2015-07-27 | 2015-10-07 | 厦门市科环海洋生物科技有限公司 | Rhodopseudomonas palustris synchronized concentration method and application |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI737210B (en) * | 2020-03-06 | 2021-08-21 | 財團法人食品工業發展研究所 | Starter culture protection formula and use thereof |
| CN113355254A (en) * | 2020-03-06 | 2021-09-07 | 财团法人食品工业发展研究所 | Formula and application of starter protective agent |
| CN113355254B (en) * | 2020-03-06 | 2024-05-03 | 财团法人食品工业发展研究所 | Formula and application of screwdriver protective agent |
| CN115851444A (en) * | 2023-03-01 | 2023-03-28 | 北京民海生物科技有限公司 | Freeze-drying protective agent for pneumococcus preservation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106754525B (en) | Lactobacillus paracasei N1115 freeze-dried powder leavening agent and preparation method thereof | |
| CN105602875B (en) | A kind of preparation method of lactic acid bacteria freeze drying powder suitable for room temperature storage | |
| CN107034149A (en) | A kind of kluyveromyces marxianus bacterium freezes the preparation method and its usage of microbial inoculum | |
| CN102220260A (en) | Lyophilization process of lactobacillus plantarum | |
| Wang et al. | Polysaccharides can improve the survival of Lactiplantibacillus plantarum subjected to freeze-drying | |
| CN104651232A (en) | Leuconostoc mesenteroides freeze-dried preparation as well as production method and application of leuconostoc mesenteroides freeze-dried preparation | |
| CN104342380A (en) | Lactobacillus freeze-drying protective agent | |
| CN102453681B (en) | Protective agent for vacuum freeze drying of lactobacillus johnsonii, and application thereof | |
| CN104611256B (en) | A kind of microorganism lyophilized formulations and preparation method thereof | |
| Spadaro et al. | Effect of culture age, protectants, and initial cell concentration on viability of freeze-dried cells of Metschnikowia pulcherrima | |
| CN106278493A (en) | The classification enzymatic isolation method preparation method containing oligosaccharide seaweed organic fertilizer | |
| CN106495827A (en) | The method that alkali carries and classification enzymolysis joint prepare the organic liquid fertilizer of ocean containing oligosaccharide | |
| CN106244459A (en) | A kind of pseudomonas dry powder and preparation method thereof | |
| CN109055227B (en) | Protective agent for freeze-drying preservation of genetically engineered strain and preservation method thereof | |
| CN109266553B (en) | Freeze-drying protective agent, method for preparing direct-injection type freeze-drying bacterial agent for puer tea by using freeze-drying protective agent and application of freeze-drying protective agent | |
| CN112457990A (en) | Freeze-drying protective agent and application thereof | |
| KR20150012445A (en) | Microorganism additives compositions for fermentation of foods with enhanced survival rate of the microorganism comprising alginate beads galic crush and lactic acid bacteria embedded therein and method of preparing the same | |
| CN112195103A (en) | Freeze-drying protective agent, coprinus comatus freeze-dried product and preparation method thereof | |
| CN104498360A (en) | Normal temperature vacuum drying protective agent for lactobacillus paracasei living bacteria agent and application method thereof | |
| KR20150125918A (en) | Microorganism additives compositions using glutinous rice paste as a cryoprotectant for fermentation of foods with enhanced survival rate of the microorganisms and method of preparing the same | |
| CN114292788A (en) | Preparation method of active probiotics by freeze drying | |
| CN103627637A (en) | Preparation method for pediococcus acidilactici strain freeze-drying preparation | |
| CN109266561B (en) | Freeze-drying protective agent, method for preparing direct-injection type freeze-drying bacterial agent for puer tea by using freeze-drying protective agent and application of freeze-drying protective agent | |
| CN107099476A (en) | It is a kind of to improve the processing method that Lactobacillus plantarum is spray-dried survival rate | |
| CN105754901A (en) | Klebsiella sp.with effect of promoting drought resistance of plants and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161221 |
|
| RJ01 | Rejection of invention patent application after publication |