CN106243243A - A kind of hyaluronic acid purifying technique - Google Patents
A kind of hyaluronic acid purifying technique Download PDFInfo
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- CN106243243A CN106243243A CN201610614516.4A CN201610614516A CN106243243A CN 106243243 A CN106243243 A CN 106243243A CN 201610614516 A CN201610614516 A CN 201610614516A CN 106243243 A CN106243243 A CN 106243243A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
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Abstract
The invention discloses a kind of hyaluronic acid purifying technique, comprise the following steps: (1) flocculation filtration；(2) activated carbon decolorizing；(3) ethanol precipitation；(4) complex-precipitation: (5) dissociate precipitation；(6) dried precipitate carried out extra-fine grinding and get final product.Hyaluronic acid purifying technique of the present invention, operating process is simple, solves the problem that current hyaluronate sodium purifying technique is complicated, and its production is easily controllable, constant product quality, and yield is high, and cost declines to a great extent, and has a good application prospect.
Description
Technical field
The present invention relates to biological polyoses extractive technique field, be specifically related to a kind of hyaluronic acid purifying technique.
Background technology
Hyaluronic acid i.e. Hyaluronic Acid (Sodium Hyaluronate), also referred to as hyaluronic acid, is called for short HA, is that the mankind were in 1934
First from bovine vitreous body, the one of isolated is widely present in animal and human connective tissue extracellular matrix and portion
Divide a kind of macromolecule straight chain acid mucopolysaccharide in antibacterial.At present, people from connective tissue, umbilical cord, skin, knuckle synovia,
The tissue such as brain, cartilage, cockscomb, Rana sylvatica Le conte skin isolates HA.In organism, HA often combines with protein, and glues with other
Polysaccharide coexists.In vitreum and synovial fluid, HA exists with dissolved form, in cockscomb and umbilical cord, exists with gel form.
HA be alternately and repeatedly to be connected by β-1,4 and β-1,3 glycosidic bond by N-Acetyl-D-glucosamine and glucuronic acid and
A kind of chain high molecular polymer become, molecular weight is between 10 ten thousand to 400 million.In molecule, two kinds of monosaccharide compare group by equimolar
Become.Owing to each dissacharide units in HA molecule all containing a carboxyl, anion the most all can be dissociated into, etc.
The anion of space length is mutually exclusive so that it is molecule is in fluffy in aqueous, occupies big quantity space, therefore has
Special water retention, water holding capacity may be up to 500ml/g in theory, is described as preferable nature moisturizing factor.Due to hydrogen bond
Effect, HA molecule is in aqueous in the secondary structure of single-screw state.When HA reaches finite concentration, produce between HA molecule
Interacting, form double-spiral structure, when concentration reaches 0.1%, HA molecule can be wound around mutually, the webbed three grades of knots of shape
Structure, gives the rheological property that HA solution is special.This characteristic is that HA has viscoelasticity and plays the basis of physiological function.HA is formed
Cancellated characteristic gives its biological function and application and development prospect widely.
Production by Microorganism Fermentation hyaluronic acid uses at present technique fermentation period length, product relative molecular weight are low, product
Product printing opacity rate variance, protein content are high, glucuronic acid content is low.The form of some hyaluronic acids is threadiness, not readily dissolves.
Summary of the invention
For above-mentioned deficiency present in prior art, the technical problem to be solved is to provide a kind of hyalomitome
Acid purifying technique.
The present invention seeks to be achieved through the following technical solutions:
A kind of hyaluronic acid purifying technique, comprises the following steps:
(1) flocculation filtration: fermentation liquid uses second acid for adjusting pH to 4.5-5.5, adds flocculant, the addition of flocculant
For 18-22mg/L, flocculation temperature is 68-72 DEG C, and flocculation time is 42-48min, refilters, and obtains filtrate；
(2) activated carbon decolorizing: filtrate uses activated carbon decolorizing, the addition of activated carbon is 5.5-7.5g/L, decolouring temperature
Degree is for 68-72 DEG C, and bleaching time is 42-48min, obtains destaining solution；
(3) ethanol precipitation: destaining solution uses 1mol/L sodium hydroxide solution regulation pH to 6.0-6.5, then precipitates through ethanol,
Obtain ethanol pellet；
(4) complex-precipitation: after being dissolved with 0.08-0.12mol/L sodium chloride solution by ethanol pellet, adds chelating agent and enters
Row complex-precipitation；
(5) dissociating precipitation: complex precipitate salt dissociates, filter, filtrate is precipitated through ethanol again, finally by precipitate through second
Dehydration of alcohols, is dried；
(6) dried precipitate carried out extra-fine grinding and get final product.
In described step (1), flocculant is mixed by sodium alginate, sodium citrate, sodium gluconate, described alginic acid
Sodium, sodium citrate, the mass ratio of sodium gluconate are (1-3): (1-3): (1-3).
Chelating agent in described step (4) is prepared from by the raw material of following weight parts: Dodecyl Dimethyl Amine 1-5
Part, TPC 1-5 part, didodecyldimethylammbromide bromide 1-5 part, water 80-100 part.
Preferably, when in described step (5), precipitate salt dissociates, first wash with 0.08-0.12mol/L sodium chloride solution
2-3 time, then add 0.4-0.6mol/L sodium chloride solution isopyknic with fermentation liquid stirs and dissociate 16-19 hour.
Preferably, described dry employing is vacuum dried, and baking temperature is 45-55 DEG C, and drying time is 2-4 hour.
Hyaluronic acid purifying technique of the present invention, operating process is simple, solves current hyaluronate sodium purifying technique complicated
Problem, its production is easily controllable, constant product quality, and yield is high, and cost declines to a great extent, and has a good application prospect.
Detailed description of the invention
Each raw material introduction in embodiment:
Fermentation liquid uses number of patent application: in 201310351005.4 prepared by the method for embodiment 1, wherein in fermentation liquid thoroughly
Bright matter acid content is 1.17g/L, and in hyaluronic acid, protein content is 0.025wt%.
Acetic acid, No. CAS: 64-19-7.
Activated carbon, No. CAS: 7440-44-0, the granularity using the Beishan Mountain, Gongyi City Kou Zhuqing activated carbon factory to provide is 200 mesh
Food grade active charcoal.
Sodium hydroxide, No. CAS: 1310-73-2.
Ethanol, No. CAS: 64-17-5.
Sodium chloride, No. CAS: 7647-14-5.
Sodium alginate, No. CAS: 31581-02-9, use the food stage that Zhuhai Wei Jia food additive company limited provides
Sodium alginate.
Sodium citrate, No. CAS: 68-04-2, the model using Cologne, Jiangsu many food ingredients company limited to provide is
The food stage sodium citrate of K2814.
Sodium gluconate, No. CAS: 527-07-1, use the food stage Fructus Vitis viniferae that Jiaxing City Long Xiao Chemical Co., Ltd. provides
Sodium saccharate.
Dodecyl Dimethyl Amine, No. CAS: 112-18-5.
TPC, No. CAS: 2785-54-8.
Didodecyldimethylammbromide bromide, No. CAS: 3282-73-3.
Embodiment 1
Hyaluronic acid purifying technique, comprises the following steps:
(1) flocculation filtration: take 25L fermentation liquid and use second acid for adjusting pH to 4.7, add flocculant stirring after being heated to 70 DEG C
Mix homogeneously flocculates, and the addition of flocculant is 20mg/L, and flocculation temperature is 70 DEG C, and flocculation time is 45min, then carries out
Vacuum filtration, obtains 24L filtrate；
(2) activated carbon decolorizing: add 144g activated carbon after 24L filtrate is heated to 72 DEG C, be 100 revs/min with rotating speed and stir
Mix decolouring 45min final vacuum sucking filtration, obtain 24L destaining solution；
(3) ethanol precipitation: destaining solution 2.5mol/L sodium hydroxide solution regulates pH to 6.5, adds 72L 95% ethanol
Precipitation, obtains ethanol pellet；
(4) complex-precipitation: ethanol pellet 25L 0.1mol/L sodium chloride solution is dissolved, be 300 turns with rotating speed/
Add chelating agent under point stirring condition, after adding chelating agent, stir 5min, then arrange on mother solution after standing 1 hour, obtain complex-precipitation
Thing；
(5) dissociate precipitation: complex precipitate 0.1mol/L sodium chloride solution washs 3 times, every time washing 0.1mol/L chlorine
Changing sodium solution consumption is 1L, and then adding in precipitation in 25L 0.5mol/L sodium chloride solution with rotating speed is 150 revs/min of stirrings
Dissociating 16 hours, vacuum filtration, filtrate is precipitated 3 hours through 75L 95% ethanol again, after being dehydrated with dehydrated alcohol, is put by precipitate
In rotary vacuum dryer, it is vacuum dried 3 hours at baking temperature is 50 DEG C, obtains dried precipitate；
(6) dried precipitate uses super grinder, and (super grinder is Wuxi City flight song Machinery Co., Ltd.
The super grinder that model is CXM produced) carry out extra-fine grinding to 500nm.Obtain the nano transparent matter acid of embodiment 1.
In described step (1), flocculant is stirred for 1:1:1 in mass ratio by sodium alginate, sodium citrate, sodium gluconate
Mix homogeneously obtains.
Chelating agent in described step (4) is prepared from by the raw material of following weight parts: Dodecyl Dimethyl Amine 3
Part, TPC 3 parts, didodecyldimethylammbromide bromide 3 parts, 91 parts of water.By Dodecyl Dimethyl Amine,
TPC, didodecyldimethylammbromide bromide are added to the water and are uniformly mixed and get final product.
Embodiment 2
Substantially the same manner as Example 1, differ only in: in described step (1), flocculant is by sodium citrate, gluconic acid
Sodium is uniformly mixed for 1:1 in mass ratio and obtains.Obtain the nano transparent matter acid of embodiment 2.
Embodiment 3
Substantially the same manner as Example 1, differ only in: in described step (1), flocculant is by sodium alginate, gluconic acid
Sodium is uniformly mixed for 1:1 in mass ratio and obtains.Obtain the nano transparent matter acid of embodiment 3.
Embodiment 4
Substantially the same manner as Example 1, differ only in: in described step (1), flocculant is by sodium alginate, sodium citrate
It is uniformly mixed for 1:1 in mass ratio and obtains.Obtain the nano transparent matter acid of embodiment 4.
Embodiment 5
Substantially the same manner as Example 1, differ only in: former by following weight parts of the chelating agent in described step (3)
Material is prepared from: TPC 4.5 parts, didodecyldimethylammbromide bromide 4.5 parts, 91 parts of water.By the tetradecane
Base pyridinium chloride, didodecyldimethylammbromide bromide are added to the water and are uniformly mixed and get final product.Obtain the nanoscale of embodiment 5
Hyaluronic acid.
Embodiment 6
Substantially the same manner as Example 1, differ only in: former by following weight parts of the chelating agent in described step (3)
Material is prepared from: Dodecyl Dimethyl Amine 4.5 parts, didodecyldimethylammbromide bromide 4.5 parts, 91 parts of water.By 12
Alkyl dimethyl tertiary amide, didodecyldimethylammbromide bromide are added to the water and are uniformly mixed and get final product.Obtain receiving of embodiment 6
Meter level hyaluronic acid.
Embodiment 7
Substantially the same manner as Example 1, differ only in: former by following weight parts of the chelating agent in described step (3)
Material is prepared from: Dodecyl Dimethyl Amine 4.5 parts, TPC 4.5 parts, 91 parts of water.By dodecyl two
Methyl tertiary amine, TPC are added to the water and are uniformly mixed and get final product.Obtain the nano transparent matter of embodiment 7
Acid.
Test case 1
Nano transparent matter acid yield embodiment 1-7 obtained is added up.Computational methods: yield (%)=nanoscale
Hyaluronic acid quality × 100 in hyaluronic acid quality/25L fermentation liquid, concrete test result is shown in Table 1.
Table 1: yield result table
| Yield, % | |
| Embodiment 1 | 97.0 |
| Embodiment 2 | 89.2 |
| Embodiment 3 | 92.1 |
| Embodiment 4 | 91.3 |
| Embodiment 5 | 91.2 |
| Embodiment 6 | 89.8 |
| Embodiment 7 | 90.6 |
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (sodium alginate, sodium citrate, sodium gluconate are compounding) yield
Apparently higher than embodiment 2-4 (in sodium alginate, sodium citrate, sodium gluconate, arbitrarily the two is compounding).Comparing embodiment 1 is with real
Executing example 5-7, (Dodecyl Dimethyl Amine, TPC, didodecyldimethylammbromide bromide are multiple for embodiment 1
Join) yield is apparently higher than in embodiment 5-7 (Dodecyl Dimethyl Amine, TPC, double dimethyl
In base ammonium bromide, arbitrarily the two is compounding).
Claims (3)
1. a hyaluronic acid purifying technique, it is characterised in that comprise the following steps:
(1) flocculation filtration: fermentation liquid using second acid for adjusting pH to 4.5-5.5, adds flocculant, the addition of flocculant is
18-22mg/L, flocculation temperature is 68-72 DEG C, and flocculation time is 42-48min, refilters, and obtains filtrate；
(2) activated carbon decolorizing: filtrate uses activated carbon decolorizing, the addition of activated carbon is 5.5-7.5g/L, and bleaching temperature is
68-72 DEG C, bleaching time is 42-48min, obtains destaining solution；
(3) ethanol precipitation: destaining solution uses 1mol/L sodium hydroxide solution regulation pH to 6.0-6.5, then precipitates through ethanol, obtains
Ethanol pellet；
(4) complex-precipitation: after being dissolved with 0.08-0.12mol/L sodium chloride solution by ethanol pellet, adds chelating agent and carries out network
Close precipitation；
(5) dissociating precipitation: complex precipitate salt dissociates, filter, filtrate is precipitated through ethanol again, is finally taken off through ethanol by precipitate
Water, is dried；
(6) dried precipitate carried out extra-fine grinding and get final product.
In described step (1), flocculant is mixed by sodium alginate, sodium citrate, sodium gluconate, described sodium alginate, lemon
Lemon acid sodium, the mass ratio of sodium gluconate are (1-3): (1-3): (1-3).
Chelating agent in described step (4) is prepared from by the raw material of following weight parts: Dodecyl Dimethyl Amine 1-5 part,
TPC 1-5 part, didodecyldimethylammbromide bromide 1-5 part, water 80-100 part.
2. hyaluronic acid purifying technique as claimed in claim 1, it is characterised in that: precipitate salt solution in described step (5)
From time, first with 0.08-0.12mol/L sodium chloride solution wash 2-3 time, then add 0.4-isopyknic with fermentation liquid
In 0.6mol/L sodium chloride solution, stirring is dissociated 16-19 hour.
3. hyaluronic acid purifying technique as claimed in claim 1, it is characterised in that: described dry employing is vacuum dried, and is dried
Temperature is 45-55 DEG C, and drying time is 2-4 hour.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110734506A (en) * | 2019-11-19 | 2020-01-31 | 山东众山生物科技有限公司 | Preparation method of sodium hyaluronate |
| CN112553273A (en) * | 2020-12-26 | 2021-03-26 | 河北华曙新合药业有限公司 | Preparation process of sodium hyaluronate with ultra-small molecular weight |
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| CN101089021A (en) * | 2007-07-12 | 2007-12-19 | 华东理工大学 | Process of separating and extracting hyaluronic acid from microbial fermented liquid |
| CN101704905A (en) * | 2009-09-28 | 2010-05-12 | 安徽丰原发酵技术工程研究有限公司 | Method for extracting hyaluronic acid |
| CN104610466A (en) * | 2014-12-23 | 2015-05-13 | 上海景峰制药有限公司 | Method for reducing content of protein in sodium hyaluronate prepared by bio-extraction method and prepared sodium hyaluronate |
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2016
- 2016-07-29 CN CN201610614516.4A patent/CN106243243A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101089021A (en) * | 2007-07-12 | 2007-12-19 | 华东理工大学 | Process of separating and extracting hyaluronic acid from microbial fermented liquid |
| CN101704905A (en) * | 2009-09-28 | 2010-05-12 | 安徽丰原发酵技术工程研究有限公司 | Method for extracting hyaluronic acid |
| CN104610466A (en) * | 2014-12-23 | 2015-05-13 | 上海景峰制药有限公司 | Method for reducing content of protein in sodium hyaluronate prepared by bio-extraction method and prepared sodium hyaluronate |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110734506A (en) * | 2019-11-19 | 2020-01-31 | 山东众山生物科技有限公司 | Preparation method of sodium hyaluronate |
| CN112553273A (en) * | 2020-12-26 | 2021-03-26 | 河北华曙新合药业有限公司 | Preparation process of sodium hyaluronate with ultra-small molecular weight |
| CN112553273B (en) * | 2020-12-26 | 2022-12-09 | 河北华曙新合药业有限公司 | Preparation process of sodium hyaluronate with ultra-small molecular weight |
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