CN106199017A - One is occulted blood detection chromatographic test paper - Google Patents
One is occulted blood detection chromatographic test paper Download PDFInfo
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- CN106199017A CN106199017A CN201610854127.9A CN201610854127A CN106199017A CN 106199017 A CN106199017 A CN 106199017A CN 201610854127 A CN201610854127 A CN 201610854127A CN 106199017 A CN106199017 A CN 106199017A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/90—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving iron binding capacity of blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/79—Transferrins, e.g. lactoferrins, ovotransferrins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/805—Haemoglobins; Myoglobins
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- Hematology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
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- Food Science & Technology (AREA)
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- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides one to occult blood detection chromatographic test paper, it is that the one-step method using Colloidal Gold and chemical method to combine quickly detects chromatographic test paper.This chromatographic test paper is made up of base plate, glass fibre loading pad, monoclonal antibody gold mark pad, nitrocellulose filter, absorbent paper, chemical detection pad, glass fibre loading pad is connected with monoclonal antibody gold mark pad, monoclonal antibody gold mark pad is overlapping with nitrocellulose filter again to be connected, and nitrocellulose filter has hemoglobin monoclonal antibody reaction circular arc (T), transferrins monoclonal antibody reaction circular arc (T) and a sheep anti mouse polyclonal antibody control circular arc (C).Absorbent paper is connected with nitrocellulose filter, and chemical detection pad occupies the subregion of reagent paper, and direct overlapping with glass fibre loading pad is connected.Double-antibody method and chemical method detect occulting blood in sample simultaneously, have the features such as sensitivity height, high specificity, accuracy is high, reaction is quick.
Description
Technical field
The present invention relates to one occult blood detection chromatographic test paper, Colloidal Gold combines with chemical method, surveys more accurately
Determine the hemoglobin in gastric juice, urine or feces and transferrin content, it is judged that it is hemorrhage whether digestive tract has, can help digest
The early screening of road cancer.
Background technology
Urine, the detection of fecal occult blood have critically important valency for diagnosing chronic digestive tract hemorrhage and pernicious large bowel neoplasm
Value, the detection that especially gastric juice is occulted blood contribute to Diagnosis of Gastric hemorrhage and screening early gastric cancer, the method that current routine examination is occulted blood
Predominantly immunization and chemical method two kinds.
The index of immunization detection is hemoglobin and transferrins, wherein, hemoglobin detection high specificity, sensitivity
Height, but poor stability, in fact it could happen that false negative phenomenon.And transferrins detection, sensitivity and specificity are examined with hemoglobin
Survey quite, but stability is apparently higher than hemoglobin, and the hemoglobin in tested specimen can be overcome in gastric transit time mistake
Long and the false negative result that occurs after being decomposed or destroying, current domestic immunization is more with hemoglobin detection, it is easy to make
Become hemorrhage failing to pinpoint a disease in diagnosis.
The ultimate principle of chemical method is consistent, produces hemoglobin after hemocyte is digested, and hemoglobin has porphyrin
Mechanism, has similar activity with peroxidase.Peroxide can be catalyzed and be produced in the presence of having hemoglobin
Nascent oxygen, nascent oxygen is very active, and oxidability is very strong, can make GUM GUAICE, pyramidon, o-tolidine and four
Whether the chromogen substance colour generations such as methyl biphenyl amine, can determine whether to contain in sample to occult blood according to colour developing result, and the depth of colour generation
React the number of hemoglobin, sxemiquantitative can have been reached, but be a lack of accuracy and specificity.Occult blood what detection used at present
Chemical method, in operating process except gather sample drop be added to Test paper (or detection card) upper in addition to also need to use at least one its
His detectable, as the auxiliary such as nitrite ion (containing peroxide) or developer (containing colour reagent) just can complete detection, operation side
Method is more complicated, and detect used reagent bottle etc. have pollute environment potential problems.
Applied chemistry method and two kinds of immunizations detect the transferrins in gastric juice and hemoglobin, to digestive tract hemorrhage simultaneously
The positive rate of property disease is significantly increased.
Summary of the invention
The present invention proposes one and occults blood detection chromatographic test paper, is detected by gold colloidal double-antibody sandwich Antigen Method simultaneously
Hemoglobin in urine, feces and gastric juice and transferrins, and again detect whether with chemical method, containing hemoglobin, to utilize
Two kinds of different principle detect, and judge to carry out dual checking on to result, improve accuracy, and content of occulting blood is carried out semidefinite
Amount.
Detection chromatographic test paper of occulting blood is by base plate, glass fibre loading pad, monoclonal antibody gold mark pad, nitrocellulose filter, water suction
Paper, chemical detection pad form.Glass fibre loading pad is connected with monoclonal antibody gold mark pad, and monoclonal antibody gold mark pad is handed over nitrocellulose filter again
Folded connected, nitrocellulose filter has hemoglobin monoclonal antibody reaction circular arc (T), a transferrins monoclonal antibody reaction circular arc
(T) and one sheep anti mouse polyclonal antibody controls circular arc (C).Absorbent paper is connected with nitrocellulose filter, and chemical detection pad occupies
The subregion of reagent paper, and directly overlap with glass fibre loading pad and be connected.
One or more being evenly equipped with in following material on described chemical detection pad are constituted: GUM GUAICE, adjacent connection first
Aniline, pyramidon, tetramethyl benzidine and peroxide, but preferably tetramethyl benzidine and peroxide, next to that
Rummy hole and peroxide.Chemical detection pad can be made up of one or more, can be by one or more chromogen substances and mistake
Oxide, improves accuracy.Chemical detection pad all uses breast except the edge being connected with glass fibre loading pad, other edges
Glue seals, in order to avoid chemical reaction interferes with immunoreation.
Designed detection chromatographic test paper of occulting blood has two kinds of structures, and the first structure chromatographs from outside to inside, this knot
Structure needs to extend outward two pins dropping samples, but undermost base plate made of soft, can bend.The second structure is
Chromatograph from inside to outside.
Detection chromatographic test paper of occulting blood provided by the present invention is mainly used for occulting blood the occulting blood inspection at body gastric juice of detection capsule
Survey but it also may for external, occulting blood in detection urine, feces and gastric juice.
By said structure, detection chromatographic test paper of occulting blood provided by the present invention has such beneficial effect: the most special
Property strong, highly sensitive, stability is strong, easily stores, need not drip nitrite ion the most again, and a step detection is complete, and range is wide, knot
Fruit accurately readability, and can sxemiquantitative.
Accompanying drawing explanation
Fig. 1 is the first structure chart occulted blood and detect chromatographic test paper.
Fig. 2 is the transverse cross-sectional view of Fig. 1.
Fig. 3 is the second structure chart occulted blood and detect chromatographic test paper.
Fig. 4 is the transverse cross-sectional view of Fig. 3.
Fig. 5 is the longitudinal sectional drawing of Fig. 3.
1, glass fibre loading pad, 2, monoclonal antibody gold mark pad, 3, nitrocellulose filter, 4, hemoglobin monoclonal antibody reaction circular arc,
5, transferrins monoclonal antibody reaction circular arc, 6, sheep anti mouse polyclonal antibody control circular arc, 7, chemical detection pad, 8, absorbent paper, 9, the end
Plate
Detailed description of the invention
Being further described the detailed description of the invention of the present invention with embodiment below in conjunction with the accompanying drawings, following example are only used
In the technical method of the present invention is clearly described, and can not limit the scope of the invention with this.
As it is shown in figure 1, the technical method that the present invention is embodied as is: time in gastric juice occults blood detection capsule, by reagent paper
Bipod along the outward flange bending of circular reagent paper, be placed in gastric juice and occult blood in detection capsule, respond the side of colour developing change
Towards camera lens, in order to shooting image.First gastric juice soak reagent paper after entering capsule by the aperture of detection capsule one end of occulting blood
Bipod, due to capillarity, gastric juice moves to central point from outside to inside along reagent paper.When with hemoglobin in gastric juice, turning ferrum
During albumen, two reaction circular arcs and control circular arc on reagent paper all can redden, and chemical detection pad also there will be color reaction,
Can carry out occulting blood sxemiquantitative according to the depth of chemistry colour generation, this result is expressed as the positive；Occult blood if gastric juice does not exist, then try
Only controlling circular arc on paper to redden, other all do not have color reaction, and this result is expressed as feminine gender；If controlling ring not redden, then table
Show that reagent paper is expired or misoperation, need again to detect.When external, only sample need to be dripped two glass at reagent paper respectively
On glass fiber loading pad, waiting reaction result, the determination methods of result is ibid.
Fig. 3 is the second structure of detection chromatographic test paper of occulting blood, and chromatography direction is from inside to outside, occults blood inspection for gastric juice
When surveying in capsule, it is desirable to have the through hole of capsule side is connected by a kind of specific connection material with the loading pad in the middle of reagent paper, this
Sample gastric juice will move to the periphery from centre.But the chemical detection in this reagent paper is lined with two pieces, left and right, can with two kinds different
Chromogen substance, such result is more accurate, and the position being only connected with loading pad seals without latex, and other positions will
Seal with latex, isolate with the antibody on nitrocellulose filter.When in gastric juice with hemoglobin, transferrins, on reagent paper
Two reaction rings and control ring and all can redden, and the chemical detection pad of both sides also there will be color reaction, can be according to change
The depth learning colour generation is carried out occulting blood sxemiquantitative, and this result is expressed as the positive；Occult blood if gastric juice does not exist, then only control on reagent paper
Ring processed reddens, and this result is expressed as feminine gender；Do not redden if controlling ring, then it represents that reagent paper is expired or misoperation, needs again to examine
Survey.When external, only sample drop need to be added on the glass fibre loading pad in the middle of reagent paper, wait reaction result, result
Determination methods is ibid.
Claims (9)
1. occult blood a detection chromatographic test paper, fine including glass fibre loading pad (1), base plate (9), monoclonal antibody gold mark pad (2), nitric acid
Dimension element film (3), absorbent paper (8), chemical detection pad (7), described glass fibre loading pad (1), monoclonal antibody gold mark pad (2), nitric acid fibre
Dimension element film (3), absorbent paper (8), chemical detection pad (7) are attached on base plate (9), and described nitrocellulose filter has one on (3)
Hemoglobin monoclonal antibody reaction circular arc (4), transferrins monoclonal antibody reaction circular arc (5) and a sheep anti mouse polyclonal antibody control
Circular arc (6).Absorbent paper is connected with nitrocellulose filter, and chemical detection pad occupies the subregion of reagent paper, and direct and glass fibers
Dimension loading pad is overlapping to be connected.Described glass fibre loading pad (1) is overlapping with monoclonal antibody gold mark pad (2) side to be connected, described monoclonal antibody gold
Mark pad (2) is overlapping with nitrocellulose filter (3) to be connected.
Detection chromatographic test paper of occulting blood the most according to claim 1, it is characterised in that be evenly equipped with on chemical detection pad selected from
One or more in lower material are constituted: GUM GUAICE, o-tolidine, pyramidon, tetramethyl benzidine and peroxidating
Thing.
Detection chromatographic test paper of occulting blood the most according to claim 1, it is characterised in that chemical detection pad except with glass fibre
The edge that loading pad is connected, other edges all seal with latex.
Detection chromatographic test paper of occulting blood the most according to claim 1, it is characterised in that reagent paper is mainly used in detection capsule of occulting blood
Occult blood detection at body gastric juice.
Detection chromatographic test paper of occulting blood the most according to claim 1, it is characterised in that chemical detection pad can be by one piece or many
Block forms, with one or more chemistry chromogen substance and peroxide.
Detection chromatographic test paper of occulting blood the most according to claim 1, it is characterised in that reagent paper has two kinds of structures, the first structure
With the second structure.
Detection chromatographic test paper of occulting blood the most according to claim 6, it is characterised in that the reagent paper of the first structure is by outer
Inside carry out chromatographing.
Detection chromatographic test paper of occulting blood the most according to claim 6, it is characterised in that the whole reagent paper of reagent paper of the first structure
Orlop is base plate, but made of soft, the part beyond border circular areas can bend.
Detection chromatographic test paper of occulting blood the most according to claim 6, it is characterised in that the reagent paper of the second structure be by interior and
Carry out outward chromatographing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610854127.9A CN106199017A (en) | 2016-09-27 | 2016-09-27 | One is occulted blood detection chromatographic test paper |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610854127.9A CN106199017A (en) | 2016-09-27 | 2016-09-27 | One is occulted blood detection chromatographic test paper |
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| Publication Number | Publication Date |
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| CN106199017A true CN106199017A (en) | 2016-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610854127.9A Pending CN106199017A (en) | 2016-09-27 | 2016-09-27 | One is occulted blood detection chromatographic test paper |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113533704A (en) * | 2021-06-21 | 2021-10-22 | 南京市第一医院 | Vomit occult blood detection device and method |
| CN115932269A (en) * | 2022-12-26 | 2023-04-07 | 江阴天泽医学检验实验室有限公司 | Method for quantitative detection of antigen by using immunochromatography colloidal gold reagent |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200956029Y (en) * | 2006-03-02 | 2007-10-03 | 万华普曼生物工程有限公司 | Transferrin colloidalgold test paper |
| US20080227208A1 (en) * | 2007-03-16 | 2008-09-18 | Hsiao-Ching Yee | Devices and Methods for Detection of Occult Blood |
| CN201522494U (en) * | 2009-04-03 | 2010-07-07 | 天津中新科炬生物制药有限公司 | Duplex occult blood fast diagnositic and chromatographic test paper |
| CN201607444U (en) * | 2010-02-08 | 2010-10-13 | 天津中新科炬生物制药有限公司 | Chromatograph test paper for fast diagnosing occult blood |
| CN104515859A (en) * | 2014-12-17 | 2015-04-15 | 杭州慧缘泰医疗器械有限公司 | Hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit and preparation method and detection method thereof |
| CN204347034U (en) * | 2014-12-17 | 2015-05-20 | 杭州慧缘泰医疗器械有限公司 | Haemoglobin, Hb-Hp, transferrins joint inspection kit |
| CN204789580U (en) * | 2015-06-04 | 2015-11-18 | 天津起跑线生物信息技术有限公司 | Jointly detect and use test paper strip |
| CN206074619U (en) * | 2016-09-27 | 2017-04-05 | 重庆大学 | One kind is occulted blood quick diagnosis chromatographic test paper |
-
2016
- 2016-09-27 CN CN201610854127.9A patent/CN106199017A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200956029Y (en) * | 2006-03-02 | 2007-10-03 | 万华普曼生物工程有限公司 | Transferrin colloidalgold test paper |
| US20080227208A1 (en) * | 2007-03-16 | 2008-09-18 | Hsiao-Ching Yee | Devices and Methods for Detection of Occult Blood |
| CN201522494U (en) * | 2009-04-03 | 2010-07-07 | 天津中新科炬生物制药有限公司 | Duplex occult blood fast diagnositic and chromatographic test paper |
| CN201607444U (en) * | 2010-02-08 | 2010-10-13 | 天津中新科炬生物制药有限公司 | Chromatograph test paper for fast diagnosing occult blood |
| CN104515859A (en) * | 2014-12-17 | 2015-04-15 | 杭州慧缘泰医疗器械有限公司 | Hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit and preparation method and detection method thereof |
| CN204347034U (en) * | 2014-12-17 | 2015-05-20 | 杭州慧缘泰医疗器械有限公司 | Haemoglobin, Hb-Hp, transferrins joint inspection kit |
| CN204789580U (en) * | 2015-06-04 | 2015-11-18 | 天津起跑线生物信息技术有限公司 | Jointly detect and use test paper strip |
| CN206074619U (en) * | 2016-09-27 | 2017-04-05 | 重庆大学 | One kind is occulted blood quick diagnosis chromatographic test paper |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113533704A (en) * | 2021-06-21 | 2021-10-22 | 南京市第一医院 | Vomit occult blood detection device and method |
| CN115932269A (en) * | 2022-12-26 | 2023-04-07 | 江阴天泽医学检验实验室有限公司 | Method for quantitative detection of antigen by using immunochromatography colloidal gold reagent |
| CN115932269B (en) * | 2022-12-26 | 2023-11-07 | 江阴天泽医学检验实验室有限公司 | Method for carrying out antigen quantitative detection by adopting immunochromatography colloidal gold reagent |
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Application publication date: 20161207 |