CN106198657A - A kind of preparation method of the parathion sensor built based on two-dimension nano materials - Google Patents

A kind of preparation method of the parathion sensor built based on two-dimension nano materials Download PDF

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CN106198657A
CN106198657A CN201610521134.7A CN201610521134A CN106198657A CN 106198657 A CN106198657 A CN 106198657A CN 201610521134 A CN201610521134 A CN 201610521134A CN 106198657 A CN106198657 A CN 106198657A
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parathion
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tio
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CN106198657B (en
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张勇
马洪敏
杜斌
胡丽华
庞雪辉
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University of Jinan
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract

The invention discloses the preparation method of a kind of electrogenerated chemiluminescence parathion sensor.Belong to Nano-function thin films and biosensor technology field.The method comprises the steps of firstly, preparing the two-dimensional nano composite Co TiO of a kind of New Two Dimensional nano composite material titanium dioxide/molybdenum bisuphide composite, i.e. cobalt doped nano titania square In-situ reaction molybdenum bisuphide2/MoS2, utilizing the good biocompatibility of this material and big specific surface area, parathion antibody in load, when detecting, owing to cobalt doped titanium dioxide can be with catalyzing hydrogen peroxide in-situ preparation O2, and with the K in end liquid2S2O8It is electrochemically reacted, produce electrochemiluminescence signal, recycling antibody is combined the impact on electron transport ability with the specific quantification of antigen, current intensity is reduced, thus reduce luminous intensity, finally achieve the structure of the Electrochemiluminescsensor sensor using unmarked electrogenerated chemiluminescence method detection parathion.

Description

A kind of preparation method of the parathion sensor built based on two-dimension nano materials
Technical field
The present invention relates to the preparation method of a kind of electrogenerated chemiluminescence parathion sensor.Belong to Nano-function thin films With biosensor technology field.
Background technology
Parathion is a kind of broad spectrum activity height poison insecticide, have tag, stomach toxicity, fumigation action, and can penetrate into plant In.Parathion to insecticide effect quickly, can be used for preventing and treating fruit tree pest insect and the wheat red mites etc. such as Cotton Gossypii, Fructus Mali pumilae, mandarin orange, pears, Fructus Persicae. In soil, parathion slightly can be migrated to deep subsoil by the eluviation of water.Parathion in soil can be by planting Thing root absorption and enter in plant.People eat by mistake this kind of plant or containing its residue plant after, parathion can pass through Digestive tract, respiratory tract and complete skin and mucosa enter human body, it may appear that Nausea and vomiting, have a headache, have loose bowels, whole body weakness nothing The preliminary symptom of poisoning such as power, is eaten for a long time or excess eats and can cause canceration.
At present, the method for detection parathion mainly has chromatography, mass spectrography etc..This type of method instrument is valuable, operation complexity, Laboratory personnel just can detect after needing professional training.Therefore, R&D costs are low, it is fast, highly sensitive, high specificity to detect Parathion sensor is significant.
Electrochemiluminescsensor sensor is widely used in due to advantages such as it are highly sensitive, specificity is good, easy and simple to handle The fields such as clinical diagnosis, pharmaceutical analysis, environmental monitoring.The Electrochemiluminescsensor sensor that processability is superior, its most critical skill Art is exactly the raising of the performances such as the most fixing and repeatability of luminous intensity and stability and immune molecule.Titanium dioxide is application Most commonly used a kind of photocatalyst material, owing to sheet-like titanium dioxide nanomaterial can expose more high miller index surface, Having higher photocatalytic activity, titanium dioxide nanoplate has ratio nanoparticle preferably application prospect, for titanium dioxide The research of nanometer sheet also receives much concern.Meanwhile, titanium dioxide poorly conductive also limit by single titanium dioxide nano material structure The sensitivity of the Electrochemiluminescsensor sensor built is the highest, is unfavorable for actual application.But, on semiconductor nano material Modify or compound special nano material, the Resonance energy transfer of semiconductor surface can be effectively improved, produce higher luminescence Intensity, and it is greatly improved detection sensitivity.Therefore, design, prepare efficient, stable titanium dioxide nanoplate and trim is Prepare the key technology of Electrochemiluminescsensor sensor.
(chemical formula is MoS to molybdenum bisuphide2) nano material, there is two-dimensional layered structure, be most widely used solid profit One of lubrication prescription.Lamellar two-dimension nano materials after its stripping, is the semiconductor nano material of excellent performance, except having big ratio Surface area, can improve load capacity as catalyst and the carrier of biological antibody, also has simultaneously as promoter excellent Electron transmission performance.
At present, most synthesizing mean be all be separately synthesized after, then catalyst is combined with carrier, process is numerous Trivial, productivity is the highest.Therefore, In-situ reaction preparation is had to the two-dimensional nano composite tool of excellent electrogenerated chemiluminescence performance Have wide practical use and important scientific meaning.
Summary of the invention
It is an object of the invention to provide a kind of prepare simple, highly sensitive, detection quickly, the electroluminescent chemistry of high specificity The preparation method of luminescence sensor, prepared sensor, can be used for quick, the Sensitive Detection of parathion.Based on this purpose, this First invention is prepared for a kind of New Two Dimensional nano composite material titanium dioxide/molybdenum bisuphide composite, i.e. cobalt doped The two-dimensional nano composite Co-TiO of nano titania square In-situ reaction molybdenum bisuphide2/MoS2, utilize the good of this material Good biocompatibility and big specific surface area, parathion antibody in load, when detecting, due to cobalt doped titanium dioxide Can be with catalyzing hydrogen peroxide in-situ preparation O2, and with the K in end liquid2S2O8It is electrochemically reacted, produces electrogenerated chemiluminescence letter Number, recycling antibody is combined the impact on electron transport ability with the specific quantification of antigen so that current intensity reduces, thus Reduce luminous intensity, finally achieve and use the electrogenerated chemiluminescence of unmarked electrogenerated chemiluminescence method detection parathion to pass The structure of sensor.
The technical solution used in the present invention is as follows:
1. the preparation method of parathion sensor built based on two-dimension nano materials, it is characterised in that described to sulfur Phosphorus sensor is made up of working electrode, two-dimension nano materials, parathion antibody, bovine serum albumin；Described two-dimensional nano material Material is the two-dimensional nano composite Co-TiO of cobalt doped nano titania square In-situ reaction molybdenum bisuphide2/MoS2；
It is characterized in that, described preparation method includes following preparation process:
A. Co-TiO is prepared2/MoS2；
B. parathion sensor is prepared；
Wherein, step a prepares Co-TiO2/MoS2Concretely comprise the following steps:
(1) take 0.6 g molybdenum disulfide powder and 0.2 ~ 2.0 mmol cobalt salt joins 3 ~ 10 mL n-butyllithium solutions jointly In, at nitrogen protection and 30 ~ 60 DEG C, stir 12 ~ 48 hours, obtain reacted solution；
(2) utilize reacted solution in non-polar solven washing step (1), at 30 ~ 60 DEG C, then carry out water bath sonicator Process, after having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum bisuphide of cobalt intercalation Nano material；
(3) the molybdenum disulfide nano material taking the cobalt intercalation that 10 ~ 500 mg steps (2) prepare joins 5 mL butyl titanates In, after stirring 1 hour, it is slowly added to 0.5 ~ 0.8 mL Fluohydric acid. while stirring, then at 160 ~ 200 DEG C in a kettle. React 18 ~ 24 hours；
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done Dry, i.e. prepare Co-TiO2/MoS2；
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L；
Described cobalt salt is selected from one of following: cobaltous sulfate, cobaltous chloride, cobalt nitrate, cobalt acetate, organic cobalt compounds；
Described non-polar solven is selected from one of following: hexane, hexamethylene, carbon tetrachloride, benzene, toluene；
Described water bath sonicator processes, and the process time is 1 hour；
Step b prepares concretely comprising the following steps of parathion sensor:
(1) with ITO electro-conductive glass as working electrode, at the Co-TiO of electrode surface drop coating 8 ~ 12 L2/MoS2Colloidal sol, under room temperature Dry；
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 8 ~ 12 L 10 The parathion antibody-solutions of g/mL, preserves in 4 DEG C of refrigerators and dries；
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 8 ~ 12 L concentration is 100 g/ The bovine serum albumin solution of mL, preserves in 4 DEG C of refrigerators and dries；
(4) the electrode PBS that will obtain in step (3), preserves after drying in 4 DEG C of refrigerators, i.e. prepares parathion and passes Sensor；
Described Co-TiO2/MoS2Colloidal sol is by the Co-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and ultrasonic The hydrosol prepared after 30 min；
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The application of the parathion sensor prepared by preparation method the most of the present invention, it is characterised in that include as follows Applying step:
A. standard solution preparation: prepare the parathion standard solution of one group of variable concentrations including blank standard specimen；
B. working electrode modify: by the parathion sensor prepared by preparation method as claimed in claim 1 be work electricity Pole, by the parathion standard solution of the variable concentrations of preparation in step a, drop coating is to working electrode surface respectively, in 4 DEG C of refrigerators Preserve；
C. working curve is drawn:, repaiied with step b as reference electrode, platinum electrode as to electrode by Ag/AgCl electrode The working electrode composition three-electrode system adornd, is connected on electrochemiluminescdetection detection equipment；Successively add in a cell The K of 15 mL2S2O8Solution and the H of 100 L2O2Solution；Cyclical voltage is applied with the cyclic voltammetry working electrode to assembling； Relation between light signal strength and the parathion antigen concentration of standard solution of the electrogenerated chemiluminescence according to gained, drawing Curve；The light signal strength of blank standard specimen is designated asD 0, the light signal strength of the parathion standard solution containing variable concentrations is designated asD i, the difference that response light signal intensity reduces is ΔD = D 0-D i, ΔDMass concentration with parathion standard solutionCBetween become Linear relationship, draws ΔD-CWorking curve；
D. the detection of parathion: replace the parathion standard solution in step a with testing sample, according to the side in step b and c Method detects, the difference DELTA reduced according to response light signal intensityDAnd working curve, obtain containing of parathion in testing sample Amount；
Described K2S2O8Solution is by 1 mol K2S2O8It is dissolved in the pH=6.5 buffer solution of 10 L with 1 mol KCl and being prepared into Arrive, described pH=6.5 buffer solution be pH value be the phosphate buffered solution of 6.5；
Described H2O2Solution is aqueous hydrogen peroxide solution, and the concentration of described aqueous hydrogen peroxide solution is 10%.
The useful achievement of the present invention
(1) parathion sensor of the present invention preparation is simple, easy to operate, it is achieved that quick, sensitive, high to parathion Selective enumeration method, and low cost, can be applicable to portable inspectiont, has market development prospect；
(2) present invention uses the method for In-situ reaction to be prepared for novel photocatalyst Co-TiO first2/MoS2, the method is main There are three advantages: one is, due to cobalt growth in situ on nano titania square fully and nano titania square Contact, utilizes the metal surface plasma body effect of cobalt, is effectively increased semiconductor substrate Resonance energy transfer ability, solves Although titanium dioxide nanoplate specific surface area is bigger and mesoporous characteristic is applicable to electrogenerated chemiluminescence host material, but electroluminescent The technical problem that chemiluminescence signal is unstable；Two are, due to the load characteristic and two of molybdenum bisuphide lamellar two-dimension nano materials TiOx nano square thereon fully dispersed, greatly increases electrogenerated chemiluminescence intensity, solves titanium dioxide and receive Rice sheet poorly conductive and electrogenerated chemiluminescence intensity are weak and be unfavorable for preparing the technical problem of Electrochemiluminescsensor sensor；Three It is, due to cobalt ion the most not only as intercalation material but also as reaction dopant material, finally to use the side of In-situ reaction Method achieves one pot of preparation of this composite, not only saves time, spillage of material, and makes the two of the cobalt doped of preparation TiOx nano square can preferably evenly spread to above molybdenum bisuphide lamellar two-dimension nano materials.Therefore, this material Effectively preparation, has important scientific meaning and using value；
(3) present invention is first by Co-TiO2/MoS2It is applied in the preparation of electroluminescent chemiluminescence biosensor, significantly improves The strength and stability of electrogenerated chemiluminescence, substantially increases the detection sensitivity of Electrochemiluminescsensor sensor so that electricity Cause chemiluminescence biosensor and achieve the application in real work；The application of this material, is also associated biomolecule sensor, Provide Technical Reference such as Optical Electro-Chemistry sensor, electrochemical sensor etc., there is the most potential use value.
Detailed description of the invention
Embodiment 1 Co-TiO2/MoS2Preparation
(1) take 0.6 g molybdenum disulfide powder and 0.2 mmol cobalt salt joins in 3mL n-butyllithium solution jointly, protect at nitrogen Protect with at 60 DEG C, stir 12 hours, obtain reacted solution；
(2) utilize reacted solution in non-polar solven washing step (1), at 60 DEG C, then carry out water bath sonicator process, After having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum disulfide nano material of cobalt intercalation Material；
(3) the molybdenum disulfide nano material taking the cobalt intercalation that 500 mg steps (2) prepare joins in 5 mL butyl titanates, stirs After mixing 1 hour, it is slowly added to 0.5 mL Fluohydric acid. while stirring, then reacts 18 hours in a kettle. at 160 DEG C；
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done Dry, i.e. prepare Co-TiO2/MoS2；
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L；
Described cobalt salt is cobaltous sulfate；
Described non-polar solven is hexane；
Described water bath sonicator processes, and the process time is 1 hour.
Embodiment 2 Co-TiO2/MoS2Preparation
(1) take 0.6 g molybdenum disulfide powder and 1.0 mmol cobalt salts join in 5 mL n-butyllithium solutions jointly, protect at nitrogen Protect with at 30 DEG C, stir 24 hours, obtain reacted solution；
(2) utilize reacted solution in non-polar solven washing step (1), at 30 DEG C, then carry out water bath sonicator process, After having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum disulfide nano material of cobalt intercalation Material；
(3) the molybdenum disulfide nano material taking the cobalt intercalation that 200 mg steps (2) prepare joins in 5 mL butyl titanates, stirs After mixing 1 hour, it is slowly added to 0.6 mL Fluohydric acid. while stirring, then reacts 20 hours in a kettle. at 180 DEG C；
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done Dry, i.e. prepare Co-TiO2/MoS2；
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L；
Described cobalt salt is cobaltous chloride；
Described non-polar solven is carbon tetrachloride；
Described water bath sonicator processes, and the process time is 1 hour.
Embodiment 3 Co-TiO2/MoS2Preparation
(1) take 0.6 g molybdenum disulfide powder and 2.0 mmol cobalt salts join in 10 mL n-butyllithium solutions, at nitrogen jointly Protection, with at 50 DEG C, is stirred 48 hours, is obtained reacted solution；
(2) utilize reacted solution in non-polar solven washing step (1), at 50 DEG C, then carry out water bath sonicator process, After having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum disulfide nano material of cobalt intercalation Material；
(3) the molybdenum disulfide nano material taking the cobalt intercalation that 10 mg steps (2) prepare joins in 5 mL butyl titanates, stirs After mixing 1 hour, it is slowly added to 0.8 mL Fluohydric acid. while stirring, then reacts 24 hours in a kettle. at 200 DEG C；
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done Dry, i.e. prepare Co-TiO2/MoS2；
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L；
Described cobalt salt is cobalt acetate；
Described non-polar solven is benzene；
Described water bath sonicator processes, and the process time is 1 hour.
The preparation method of embodiment 4 parathion sensor
(1) using a width of 1 cm, a length of 4 cm ITO electro-conductive glass as working electrode, at the Co-of electrode surface drop coating 8 L TiO2/MoS2Colloidal sol, dries under room temperature；
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 8 L 10 g/mL Parathion antibody-solutions, 4 DEG C of refrigerators preserve and dry；
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 8 L concentration is 100 g/mL Bovine serum albumin solution, 4 DEG C of refrigerators preserve and dry；
(4) the electrode PBS that will obtain in step (3), preserves in 4 DEG C of refrigerators after drying, and i.e. prepares parathion sensing Device；
Described Co-TiO2/MoS2Colloidal sol is by the Co-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and ultrasonic The hydrosol prepared after 30 min；
Described PBS is the phosphate buffered solution of 10mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The preparation method of embodiment 5 parathion sensor
(1) using a width of 1 cm, a length of 4 cm ITO electro-conductive glass as working electrode, at the Co-of electrode surface drop coating 10 L TiO2/MoS2Colloidal sol, dries under room temperature；
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 10 L 10 g/ The parathion antibody-solutions of mL, preserves in 4 DEG C of refrigerators and dries；
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 10 L concentration is 100 g/mL Bovine serum albumin solution, 4 DEG C of refrigerators preserve and dry；
(4) the electrode PBS that will obtain in step (3), preserves after drying in 4 DEG C of refrigerators, i.e. prepares parathion and passes Sensor；
Described Co-TiO2/MoS2Colloidal sol is by the Co-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and ultrasonic The hydrosol prepared after 30 min；
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The preparation method of embodiment 6 parathion sensor
(1) using a width of 1 cm, a length of 4 cm ITO electro-conductive glass as working electrode, at the Co-of electrode surface drop coating 12 L TiO2/MoS2Colloidal sol, dries under room temperature；
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 12 L 10 g/ The parathion antibody-solutions of mL, preserves in 4 DEG C of refrigerators and dries；
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 12 L concentration is 100 g/mL Bovine serum albumin solution, 4 DEG C of refrigerators preserve and dry；
(4) the electrode PBS that will obtain in step (3), preserves after drying in 4 DEG C of refrigerators, i.e. prepares parathion and passes Sensor；
Described Co-TiO2/MoS2Colloidal sol is by the Co-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and ultrasonic The hydrosol prepared after 30 min；
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
The parathion sensor of embodiment 7 embodiment 1 ~ 6 preparation, is applied to the detection of parathion, and step is as follows:
(1) standard solution preparation: prepare the parathion standard solution of one group of variable concentrations including blank standard specimen；
(2) working electrode modify: by the parathion sensor prepared by preparation method as claimed in claim 1 be work electricity Pole, by the drop coating respectively of the parathion standard solution of the variable concentrations of preparation in step (1) to working electrode surface, 4 DEG C of refrigerators Middle preservation；
(3) working curve is drawn: using saturated calomel electrode as reference electrode, platinum electrode is as auxiliary electrode, with step (2) The working electrode composition three-electrode system modified, is connected on electrochemiluminescdetection detection equipment；In a cell successively Add the K of 15 mL2S2O8Solution and the H of 100 L2O2Solution；Circulation electricity is applied with the cyclic voltammetry working electrode to assembling Pressure；Relation between light signal strength and the parathion antigen concentration of standard solution of the electrogenerated chemiluminescence according to gained, draws Working curve；The light signal strength of blank standard specimen is designated asD 0, the light signal strength of the parathion standard solution containing variable concentrations It is designated asD i, the difference that response light signal intensity reduces is ΔD = D 0-D i, ΔDMass concentration with parathion standard solutionCIt Between linear, draw ΔD-CWorking curve；The linear detection range of parathion is: 0.003 ~ 100 ng/mL, detection limit For: 1.1 pg/mL；
(4) detection of parathion: replace the parathion standard solution in step a with testing sample, according to the side in step b and c Method detects, the difference DELTA reduced according to response light signal intensityDAnd working curve, obtain containing of parathion in testing sample Amount；
Described K2S2O8Solution is by 1 mol K2S2O8It is dissolved in the pH=6.5 buffer solution of 10 L with 1 mol KCl and being prepared into Arrive, described pH=6.5 buffer solution be pH value be the phosphate buffered solution of 6.5；
Described H2O2Solution is aqueous hydrogen peroxide solution, and the concentration of described aqueous hydrogen peroxide solution is 10%.

Claims (4)

1. the preparation method of the parathion sensor built based on two-dimension nano materials, it is characterised in that described parathion Sensor is made up of working electrode, two-dimension nano materials, parathion antibody, bovine serum albumin；Described two-dimension nano materials Two-dimensional nano composite Co-TiO for cobalt doped nano titania square In-situ reaction molybdenum bisuphide2/MoS2。
2. the preparation method of the parathion sensor built based on two-dimension nano materials as claimed in claim 1, its feature exists Following two preparation process is included in, described preparation method:
A. Co-TiO is prepared2/MoS2；
B. parathion sensor is prepared.
3. the preparation method of the parathion sensor built based on two-dimension nano materials as described in claim 1 and 2, its feature It is, described Co-TiO2/MoS2Concrete preparation process be:
(1) take 0.6 g molybdenum disulfide powder and 0.2 ~ 2.0 mmol cobalt salt joins 3 ~ 10 mL n-butyllithium solutions jointly In, at nitrogen protection and 30 ~ 60 DEG C, stir 12 ~ 48 hours, obtain reacted solution；
(2) utilize reacted solution in non-polar solven washing step (1), at 30 ~ 60 DEG C, then carry out water bath sonicator Process, after having processed, the solution after recycling non-polar solven carrying out washing treatment, vacuum drying, obtain the molybdenum bisuphide of cobalt intercalation Nano material；
(3) the molybdenum disulfide nano material taking the cobalt intercalation that 10 ~ 500 mg steps (2) prepare joins 5 mL butyl titanates In, after stirring 1 hour, it is slowly added to 0.5 ~ 0.8 mL Fluohydric acid. while stirring, then at 160 ~ 200 DEG C in a kettle. React 18 ~ 24 hours；
(4) by the product of step (3) gained, after ultra-pure water and dehydrated alcohol centrifuge washing three times, at 50 DEG C, vacuum is done Dry, i.e. prepare Co-TiO2/MoS2；
Described n-butyllithium solution is the hexane solution of n-BuLi, and concentration is 1.6 mol/L；
Described cobalt salt is selected from one of following: cobaltous sulfate, cobaltous chloride, cobalt nitrate, cobalt acetate, organic cobalt compounds；
Described non-polar solven is selected from one of following: hexane, hexamethylene, carbon tetrachloride, benzene, toluene；
Described water bath sonicator processes, and the process time is 1 hour.
4. the preparation method of the parathion sensor built based on two-dimension nano materials as described in claim 1 and 2, its feature Being, the concrete preparation process of described parathion sensor is:
(1) with ITO electro-conductive glass as working electrode, at the Co-TiO of electrode surface drop coating 8 ~ 12 L2/MoS2Colloidal sol, under room temperature Dry；
(2) the electrode buffer solution PBS that will obtain in step (1), continues at electrode surface drop coating 8 ~ 12 L 10 The parathion antibody-solutions of g/mL, preserves in 4 DEG C of refrigerators and dries；
(3) the electrode PBS that will obtain in step (2), continuing in electrode surface drop coating 8 ~ 12 L concentration is 100 g/ The bovine serum albumin solution of mL, preserves in 4 DEG C of refrigerators and dries；
(4) the electrode PBS that will obtain in step (3), preserves after drying in 4 DEG C of refrigerators, i.e. prepares parathion and passes Sensor；
Described Co-TiO2/MoS2Colloidal sol is by the Co-TiO of 50 mg2/MoS2Powder is dissolved in 10 mL ultra-pure waters, and ultrasonic The hydrosol prepared after 30 min；
Described PBS is the phosphate buffered solution of 10 mmol/L, and the pH value of described phosphate buffered solution is 7.4.
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