CN106192020A - A kind of method that microorganism builds the flora storehouse transplanted for flora when utilizing autologous health status - Google Patents
A kind of method that microorganism builds the flora storehouse transplanted for flora when utilizing autologous health status Download PDFInfo
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Abstract
A kind of method that microorganism builds the flora storehouse transplanted for flora when utilizing autologous health status, its operating procedure is as follows: 1, depositor's information gathering and the confirmation of health status；2, the collection of autologous region of interest microorganism species；3, microorganism species diversity analysis；4, the preservation of microorganism species；5, in microorganism species, the separation of probiotic bacteria preserves；6, Jian Ku and the information system management in storehouse.The microorganism preserved when utilizing autologous health status carries out transplantation treatment disease, health care etc., compares the microorganism transplanting other people, has psychological acceptance strong, without immunologic rejection, without advantages such as dispute of ethics.Be established as future individualized accurate medical treatment, the health care etc. in Healthy People autologous microorganism species storehouse provide basis.
Description
Technical field
The present invention is that the autologous microorganism utilizing people when health status builds the flora storehouse transplanted for flora, in the future
Transplanted by flora and treat disease, health care etc., relate to microbiology, accurate medical domain.
Background technology
Scientific investigations showed that, the microbial cell quantity lived in human body is likely to be breached 1014, exceed human body cell number
More than 10 times, gene dosage of its coding is more than 100 times of human genome gene dosage.These micropopulations are for people
Body has important physiological action, and they help us to digest food, produces various vitamin, adjusts our immune system,
Antagonism harmful microorganism, protects our skin from infection, even affects build and the emotion of people, for human diseases
Therapeutic potential great.
All live microorganism in human body many positions, wherein the micro organism quantity in intestinal at most, population the abundantest, about
The human normal microorganism of 80% all concentrates on here, and gross weight is more than one kilogram, and kind has four or five hundred kinds.On the skin of people also
Living many microorganisms (skin table microorganism), have antibacterial also to have fungus, inorganic ions and Organic substance in human sweat are these
The most biological satisfied food.Oral cavity is the good place of microbe survival, and saliva brings abundant nutrition to microorganism here
With sufficient moisture, rugged surface, oral cavity provides again the institute stayed in for each quasi-microorganism, the nearlyest hundred kinds of antibacterials, very
Bacterium and protozoacide can be lived the most serenely.Determine additionally, nasal cavity, pharyngeal cavity and female genital tract are also some microorganisms
The place occupied.Although the stomach of human body is not the good place to go of microorganism, but the most also life has a small amount of aciduric bacteria and yeast,
They stick to grow on coat of the stomach.
In healthy human body, the field planting of these microflora and ecological balance are to maintaining health to play very important
Effect.Studying discovery recently, the disease that the mankind have more than 90% is relevant with intestinal flora imbalance.Caprophyl is transplanted Healthy People
Caprophyl is transplanted in patient's gastrointestinal tract, helps correct the unbalance intestinal microbial population of patient and rebuild the micro-life of intestinal with normal function
State.At present, caprophyl is transplanted to infect Clestridium difficile and is had good efficacy and obtained extensively approval；It is for losing with flora
Inside and outside other intestinals that phase modulation closes, the therapeutical effect of disease also becomes study hotspot.Meanwhile, other position microorganism species of human body
Unbalance also caused various relevant disease, future perhaps can also be transplanted by flora and be rebuild normal Tiny ecosystem, thus reaches
To the purpose alleviating or treating disease.
But the at present selection of relevant flora transplantation donor and screening are still without seeking unity of standard, and even there is also many arguements,
The impact such as the age of donor, health assessment, the immunologic rejection of flora and safety (especially long-term safety) also needs to be correlated with
Comparative study and long term follow-up further investigated.The particularity transplanted due to flora, kinsfolk or friend are often specified to donations
Person, healthy volunteer is also suitable donor.But, transplant other people microorganism on the one hand may have the unknown safe because of
Element (such as unknown harmful microbe existence, immunological rejection, long-term safety etc.), on the other hand there is also certain ethics
Problem and psychological repellence problem, and when autologous transplanting health status especially adolescence, (feces of child is by environmental factors shadow
Ringing less, the degree of variation of its intestinal microbial population is relatively low, may also be good caprophyl source) microorganism species then can be effective
Avoid these problems.Therefore, especially child or the microorganism in teenage period during " backup " autologous health status, future is once
When occurring relevant disease to need flora transplantation treatment, so that it may do the transplanting of autologous microorganism species immediately, get well, alleviate certainly
Oneself and household's financial burden.
Research also finds, people is always first caused by intestinal beneficial microbes minimizing.Baby is several after being born in mother's abdomen
In hour, by breast-feeding and the approach such as anus, substantial amounts of beneficial microbe is just had to settle down in its intestinal, in adolescence to 40 years old
In this stage, useful mushroom is always in occupation of absolute advantages, and after 40 years old, useful mushroom quantity gradually decreases, and harmful mushroom increases
Many, intestinal mucosa is thinning, and its defensive enginery and immune function weaken, and result causes a large amount of harmful substance and noxious bacteria to produce
Toxin enters blood, damages internal organs, accelerates the functional deterioration of each internal organs, make the person the most ageing.So, application is certainly
During the health status deposited especially adolescence time intestinal microflora, it is also possible to realize " preserve beauty " dreamed of
The wonderful dream of " perpetual youth ", has high-quality life.Meanwhile, autologous microorganism species may not only be applied to me, can be also
The clinical practice of its father and mother and relevant relatives (such as hall cousin younger sister, even father and mother) et al..And also it is available for the whole nation and whole world trouble
After person carries out relevant matches, transplant selected as microorganism species and use.The structure in autologous microorganism species storehouse can be that enterprise brings
Huge income.
Summary of the invention
The present invention is that the autologous microorganism utilizing people when health status builds the flora storehouse transplanted for flora, in the future
Transplanted by flora and treat disease, health care etc., compare the microorganism transplanting other people, there is psychological acceptance strong, without immunity row
Scold, without advantages such as dispute of ethics.Be established as future individualized accurate medical treatment, the health care etc. in Healthy People autologous microorganism species storehouse carry
For basis.
A kind of method that when present invention utilizes autologous health status, microorganism builds the flora storehouse transplanted for flora,
It follows the steps below:
1, depositor's information gathering and the confirmation of health status.Depositor signs " autologous microorganism species storehouse service agreement book "
After, fill in identity information, and inquire that its history of disease of understanding (has included whether suffer from diarrhoea medical history and chronic constipation, if having pernicious swollen
Tumor history, if having systemic autoimmune disease, if abuse intravenous drug, if having history of drug abuse, if having height
Risk behavior, if tatoo and take into custody history etc.)；Afterwards it is checked UP and Psychological Evaluation, health check-up at least should comprise with
Lower content: (infectious disease known to check whether there is, serum is hepatitis A virus IgM, hepatitis B IgM to serologic test
And IgG, hbs antigen, hepatitis B surface antibody IgM, hepatitis C virus IgG antibody, HIV 1 type and 2 types,
Fluorescence Tyreponema pallidum antibody adsorption test)；Blood testing (checks whether region of interest has inflammation, checks in blood white thin
Born of the same parents, lymphocyte, intermediate cell number etc.)；Feces test (carries out PCR, antigen, microscopy and cultivation test, checks intestinal feces
The most whether there are the spores such as clostridium difficile toxin B, rotavirus, giardia lamblia, Cryptosporidium, Cyclospora, Cryptosporidium
Bulbec worm (acid-fast stain), worm's ovum, parasite, will congratulate toxin producing escherichia coli, salmonella, shigella dysenteriae, Yersinia pestis,
Campylobacter, Non-cholera vibrio, Plesiomonas, Aeromonas etc.).Psychological Evaluation uses the mode of questionnaire survey, predominantly detects
Whether there are psychology and mental sickness.Finally, determine that able-bodied depositor just advises carrying out the preservation of autologous microorganism species,
Should be well able-bodied child or minor, unsound depositor is it is not recommended that preserve.
2, the collection of autologous region of interest microorganism species.Gather position and include oral cavity, nasal cavity, female genital tract, intestinal,
Gatherer process is aseptically carried out, and preferably carries out in the room of GMP100.Oral cavity, nasal cavity, the microorganism of reproductive tract
Flora uses aseptic cotton carrier sampling, and enteric microorganism uses special dejecta sampling device to be sampled, and is immediately placed in after sampling
The anaerobic reduction liquid of sterilizing in advance seals and preserves.
3, microorganism species diversity analysis.Microbiological specimens to same batch gained, takes a sample respectively and carries out high pass
Measure sequence, understand the multiformity of its microorganism, and its testing result of keeping records.
4, the preservation of microorganism species.Put in frozen container after good for the microbiological specimens subpackage of above-mentioned gained, seal,
Numbering, and register, carrying out program the most immediately is cooling, is then placed in-196 DEG C of medium-term and long-term preservations of liquid nitrogen container, or by micro-life
Thing sample making becomes capsule to carry out freezen protective, storage condition periodic detection.
5, in microorganism species, the separation of probiotic bacteria preserves.Isolated and purified from the microbiological specimens of above-mentioned gained obtain phase
Close probiotic bacteria, such as bacillus bifidus (Bifidobacterium), lactobacillus (Lactobacill), intend bar clostridium
(Bacteroides), Clostridium leptum, bacteroides fragilis, unusual bacterium (Atopobium), general Salmonella (Prevotella) etc., then
These probiotic bacterias are respectively put into-196 DEG C of medium-term and long-term preservations of liquid nitrogen container again, or microbiological specimens is fabricated to capsule carries out cold
Freeze preservation, storage condition periodic detection.
6, Jian Ku and the information system management in storehouse.By input computer data base after sample message and depositor's information matches, and
Set up database information retrieval, be managed according to Microbiological Culture Collection library standard.
Accompanying drawing explanation
Fig. 1 be of the present invention a kind of when utilizing autologous health status microorganism build the flora transplanted for flora
The method flow diagram in storehouse.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described, in order to be better understood from the present invention, aobvious
So, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the reality in the present invention
Execute example, all other embodiments that those of ordinary skill in the art are obtained under not making creative work premise, all belong to
In the scope of protection of the invention.
Embodiment
Microorganism when utilizing autologous health status in the present embodiment builds the method in the flora storehouse transplanted for flora,
It is divided into following step to carry out.
1, depositor's information gathering and the confirmation of health status.Depositor signs " autologous microorganism species storehouse service agreement book "
After, fill in identity information, and inquire that its history of disease of understanding (has included whether suffer from diarrhoea medical history and chronic constipation, if having pernicious swollen
Tumor history, if having systemic autoimmune disease, if abuse intravenous drug, if having history of drug abuse, if having height
Risk behavior, if tatoo and take into custody history etc.)；Afterwards it is checked UP and Psychological Evaluation, health check-up at least should comprise with
Lower content: (infectious disease known to check whether there is, serum is hepatitis A virus IgM, hepatitis B IgM to serologic test
And IgG, hbs antigen, hepatitis B surface antibody IgM, hepatitis C virus IgG antibody, HIV 1 type and 2 types,
Fluorescence Tyreponema pallidum antibody adsorption test)；Blood testing (checks whether region of interest has inflammation, checks in blood white thin
Born of the same parents, lymphocyte, intermediate cell number etc.)；Feces test (carries out PCR, antigen, microscopy and cultivation test, checks intestinal feces
The most whether there are the spores such as clostridium difficile toxin B, rotavirus, giardia lamblia, Cryptosporidium, Cyclospora, Cryptosporidium
Bulbec worm (acid-fast stain), worm's ovum, parasite, will congratulate toxin producing escherichia coli, salmonella, shigella dysenteriae, Yersinia pestis,
Campylobacter, Non-cholera vibrio, Plesiomonas, Aeromonas etc.).Psychological Evaluation uses the mode of questionnaire survey, predominantly detects
Whether there are psychology and mental sickness.Finally, determine that able-bodied depositor just advises carrying out the preservation of autologous microorganism species,
Should be well able-bodied child or minor, unsound depositor is it is not recommended that preserve.
2, the collection of autologous region of interest microorganism species.Gather position and include oral cavity, nasal cavity, female genital tract, intestinal,
Gatherer process is aseptically carried out, and preferably carries out in the room of GMP100.Oral cavity, nasal cavity, the microorganism of reproductive tract
Flora uses aseptic cotton carrier sampling, and enteric microorganism uses special dejecta sampling device to be sampled, and is immediately placed in after sampling
The anaerobic reduction liquid of sterilizing in advance seals and preserves.
3, microorganism species diversity analysis.Microbiological specimens to same batch gained, takes a sample respectively and carries out high pass
Measure sequence, understand the multiformity of its microorganism, and its testing result of keeping records.
4, the preservation of microorganism species.Put in frozen container after good for the microbiological specimens subpackage of above-mentioned gained, seal,
Numbering, and register, carrying out program the most immediately is cooling, is then placed in-196 DEG C of medium-term and long-term preservations of liquid nitrogen container, or by micro-life
Thing sample making becomes capsule to carry out freezen protective, storage condition periodic detection.
5, in microorganism species, the separation of probiotic bacteria preserves.Isolated and purified from the microbiological specimens of above-mentioned gained obtain phase
Close probiotic bacteria, such as bacillus bifidus (Bifidobacterium), lactobacillus (Lactobacill), intend bar clostridium
(Bacteroides), Clostridium leptum, bacteroides fragilis, unusual bacterium (Atopobium), general Salmonella (Prevotella) etc., then
These probiotic bacterias are respectively put into-196 DEG C of medium-term and long-term preservations of liquid nitrogen container again or microbiological specimens is fabricated to capsule carries out cold
Freeze preservation, storage condition periodic detection.
6, Jian Ku and the information system management in storehouse.By input computer data base after sample message and depositor's information matches, and
Set up database information retrieval, be managed according to Microbiological Culture Collection library standard.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (7)
1. the method that microorganism when utilizing autologous health status builds the flora storehouse transplanted for flora, it is characterised in that
It is to follow the steps below:
Depositor's information gathering and the confirmation of health status, after depositor signs " autologous microorganism species storehouse service agreement book ", fill in
Identity information, history of disease, check UP afterwards；
The collection of autologous region of interest microorganism species, gathers position and includes oral cavity, nasal cavity, female genital tract, intestinal, gathered
Journey is aseptically carried out；
Microorganism species diversity analysis, carries out microbial diversity detection to sample；
The preservation of microorganism species, puts into-196 DEG C of medium-term and long-term guarantors of liquid nitrogen container by the above-mentioned region of interest microorganism species that is collected in
Deposit；
In microorganism species, the separation of probiotic bacteria preserves, and isolated and purified from the microbiological specimens of above-mentioned gained obtains known phase
Close probiotic bacteria, be then placed in-196 DEG C of medium-term and long-term preservations of liquid nitrogen container；
Jian Ku and the information system management in storehouse, by input computer data base after sample message and depositor's information matches, according to strain
Preservation library standard is managed.
2. when utilizing autologous health status according to a kind of described in claim 1, microorganism builds the bacterium transplanted for flora
The method in group storehouse, it is characterised in that: the confirmation of (1) health status: depositor signs " autologous microorganism species storehouse service agreement book "
After, fill in identity information, inquiry is understood its history of disease and (has been included whether suffer from diarrhoea medical history and chronic constipation, if having malignant tumor
Medical history, if having systemic autoimmune disease, if abuse intravenous drug, if having history of drug abuse, if having high wind
Dangerous behavior, if tatoo and take into custody history etc.)；Checking it UP afterwards and Psychological Evaluation, health check-up at least should comprise following
Content: serologic test (infectious disease known to check whether there is, serum be hepatitis A virus IgM, hepatitis B IgM and
IgG, hbs antigen, hepatitis B surface antibody IgM, hepatitis C virus IgG antibody, HIV1 type and 2 types, fluorescence
Tyreponema pallidum antibody adsorption test), blood testing (checks whether region of interest has inflammation, checks blood middle leukocytes, pouring
Bar cell, intermediate cell number etc.), feces test (carries out PCR, antigen, microscopy and cultivation test, in checking intestinal is feces
No have the spore balls such as clostridium difficile toxin B, rotavirus, giardia lamblia, Cryptosporidium, Cyclospora, Cryptosporidium
Worm (acid-fast stain), worm's ovum, parasite, will congratulate toxin producing escherichia coli, salmonella, shigella dysenteriae, Yersinia pestis, bending
Bacillus, Non-cholera vibrio, Plesiomonas, Aeromonas etc.).Whether Psychological Evaluation uses the mode of questionnaire survey, predominantly detect
There are psychology and mental sickness.Finally, the preservation of able-bodied depositor just practicable autologous microorganism species is determined, it is proposed that preferential
Selecting able-bodied child or child's (age was less than for 12 one full year of life) is conservation object, secondly selects able-bodied teenager (place
In adolescence, 13-19 one full year of life), it is able-bodied youth (20-40 one full year of life) again, unsound depositor does not preserves.
3. when utilizing autologous health status according to a kind of described in claim 1, microorganism builds the bacterium transplanted for flora
Group storehouse method, it is characterised in that: the collection of (2) autologous region of interest microorganism species: gather position include oral cavity, nasal cavity,
Female genital tract, intestinal, gatherer process is aseptically carried out, and oral cavity, nasal cavity, the microorganism species of reproductive tract use aseptic
Cotton swab samples, and enteric microorganism uses special dejecta sampling device to be sampled, and is immediately placed in detesting of sterilizing the most in advance after sampling
Hydrogen reduction liquid seals and preserves.
4. when utilizing autologous health status according to a kind of described in claim 1, microorganism builds the bacterium transplanted for flora
The method in group storehouse, it is characterised in that: (3) microorganism species diversity analysis: the microbiological specimens to same batch gained, take respectively
One sample carries out microbial diversity detection, and its testing result of keeping records.
5. when utilizing autologous health status according to a kind of described in claim 1, microorganism builds the bacterium transplanted for flora
The method in group storehouse, it is characterised in that: the preservation of (4) microorganism species: put into after good for the microbiological specimens subpackage of above-mentioned gained
In frozen container, seal, numbering, and register, carry out program mode cooling the most immediately, be then placed in-196 DEG C of liquid nitrogen containers long
Phase preserves, or microbiological specimens is fabricated to capsule carries out freezen protective, storage condition periodic detection.
6. when utilizing autologous health status according to a kind of described in claim 1, microorganism builds the bacterium transplanted for flora
The method in group storehouse, it is characterised in that: in (5) microorganism species, the separation of probiotic bacteria preserves: from the microbiological specimens of above-mentioned gained
In isolated and purified obtain relevant probiotic bacteria, such as bacillus bifidus (Bifidobacterium), lactobacillus (Lactobacill), intend
Bar clostridium (Bacteroides), Clostridium leptum, bacteroides fragilis, unusual bacterium (Atopobium), general Salmonella (Prevotella)
Deng, these probiotic bacterias are respectively put into-196 DEG C of medium-term and long-term preservations of liquid nitrogen container the most again, or microbiological specimens is made plastic
Capsule carries out freezen protective, storage condition periodic detection.
7. when utilizing autologous health status according to a kind of described in claim 1, microorganism builds the bacterium transplanted for flora
The method in group storehouse, it is characterised in that: (6) Jian Ku and the information system management in storehouse: by typing after sample message and depositor's information matches
Computer Database, and set up database information retrieval, it is managed according to Microbiological Culture Collection library standard.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111681733A (en) * | 2020-04-30 | 2020-09-18 | 厦门承葛生物科技有限公司 | Method for screening intestinal flora transplantation donor |
| CN111870617A (en) * | 2019-11-04 | 2020-11-03 | 深圳碳云智能数字生命健康管理有限公司 | Method and device for determining intestinal probiotic supplement formula, storage medium and processor |
| CN112899360A (en) * | 2021-02-02 | 2021-06-04 | 北京航空航天大学 | Application method of composition for detecting occurrence probability of Terchester-Coriolis syndrome |
| CN113337630A (en) * | 2021-08-02 | 2021-09-03 | 伯克利南京医学研究有限责任公司 | Microbial marker for evaluating curative effect of fecal bacteria transplantation of type II diabetic patients and application of microbial marker |
| CN114341370A (en) * | 2020-07-30 | 2022-04-12 | 内斯托尔株式会社 | Bacteria providing system |
| CN115983601A (en) * | 2023-01-18 | 2023-04-18 | 奇辉生物科技(扬州)有限公司 | Whole-process big data management platform for coprophilous fungus transplantation |
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| CN111681733A (en) * | 2020-04-30 | 2020-09-18 | 厦门承葛生物科技有限公司 | Method for screening intestinal flora transplantation donor |
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