CN106190926A - A kind of enterococcus faecalis fermentation medium - Google Patents

A kind of enterococcus faecalis fermentation medium Download PDF

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Publication number
CN106190926A
CN106190926A CN201610760483.4A CN201610760483A CN106190926A CN 106190926 A CN106190926 A CN 106190926A CN 201610760483 A CN201610760483 A CN 201610760483A CN 106190926 A CN106190926 A CN 106190926A
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China
Prior art keywords
enterococcus faecalis
fermentation medium
nitrogen source
peptone
sucrose
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CN201610760483.4A
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Inventor
胡国春
刘扬科
郭文江
成鹏
常鹏艳
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Linzhou Zhongnong Ying Tai Biological Peptide Co Ltd
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Linzhou Zhongnong Ying Tai Biological Peptide Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 1% 5%, peptone 0.5% 2%, biological nitrogen source 1% 5%, potassium dihydrogen phosphate 0.01% 0.1%, dipotassium hydrogen phosphate 0.1% 1%, magnesium sulfate 0.1% 1%, manganese sulfate 0.001% 0.005%, surplus is water.This culture medium achieves the low cost as microbial ecological agent enterococcus faecalis, high efficiency production.

Description

A kind of enterococcus faecalis fermentation medium
Technical field
The invention belongs to technical field of bioengineering, be specifically related to a kind of enterococcus faecalis fermentation medium.
Background technology
Enterococcus faecalis (Enterococcus Faecium) belongs to the normal flora in people and animal intestinal, after entering intestinal Can the most surely grow.Enterococcus faecalis fermentation produces lactic acid, advantageously reduces intestinal environment pH, the growth of suppression harmful bacteria.Dung intestinal ball Bacterium metabolic process also can produce the materials such as hydrogen peroxide, bacteriocin, pathogenic bacterium are had certain killing action, and does not occurs Medicine is residual.In view of the above characteristic of enterococcus faecalis, increasing producer selects it to carry out the fermenting and producing of microbial ecological agent.
Traditional lactic acid bacteria fermentation many selections MRS culture medium, expensive, the viable count after fermentation is less than 1 × 10 9 CFU/mL, in the case of the effective using dosage of probiotic bacteria is fixing, low fermentation level adds cost equal to covert.Low cost Culture medium, the fermentation technology that can amplify efficiently for advance probiotics fermention industrialization have great guidance and practice meaning Justice.
Summary of the invention
It is an object of the invention to provide a kind of enterococcus faecalis fermentation medium, this culture medium achieves as Tiny ecosystem system The low cost of agent enterococcus faecalis, high efficiency production.
For achieving the above object, the technical solution used in the present invention is as follows:
The invention provides a kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 1%- 5%, peptone 0.5%-2%, biological nitrogen source 1%-5%, potassium dihydrogen phosphate 0.01%-0.1%, dipotassium hydrogen phosphate 0.1%-1%, magnesium sulfate 0.1%-1%, manganese sulfate 0.001%-0.005%, surplus is water.
According to above-mentioned enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 1%- 3%, peptone 1.5%-2%, biological nitrogen source 1%-3%, potassium dihydrogen phosphate 0.01%-0.04%, dipotassium hydrogen phosphate 0.6%-1%, magnesium sulfate 0.4%-1%, manganese sulfate 0.004%-0.005%, surplus is water.
Enterococcus faecalis fermentation medium according to claim 1, it is made up of the component of following mass percent: Sucrose 3.1%-5%, peptone 0.5%-1.4%, biological nitrogen source 3.1%-5%, potassium dihydrogen phosphate 0.05%-0.1%, dipotassium hydrogen phosphate 0.1%-0.55%, magnesium sulfate 0.1%-0.35%, manganese sulfate 0.001%-0.003%, surplus is water.
According to above-mentioned enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 3%, egg White peptone 1%, biological nitrogen source 2%, potassium dihydrogen phosphate 0.05%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, manganese sulfate 0.002%, surplus For water.
According to above-mentioned enterococcus faecalis fermentation medium, described biological nitrogen source is mainly by aminoacid, vitamin and mineral Composition.
According to above-mentioned enterococcus faecalis fermentation medium, in the composition in described biological nitrogen source, dry is more than 70%, and total nitrogen is big In 8%, amino-acid nitrogen is more than 2%, and ash is less than 15%, pH value 5.0-6.5.
Compared with prior art, beneficial effects of the present invention:
Culture medium of the present invention contains biological nitrogen source, it is possible to accelerates enterococcus faecalis and rapidly enters exponential phase, fermentation liquid viable count Averagely can improve more than 30%.Fermentation period can be made to shorten 2-3 hour, thus reduce production cost.
Detailed description of the invention
Following example are intended to further illustrate present disclosure, but are not intended to protection scope of the present invention.
Following raw material sucrose, peptone, biological nitrogen source, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate and manganese sulfate are Commercially available；The producer in biological nitrogen source be Xuzhou City be a day Science and Technology Ltd..
Embodiment 1
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 1%-5%, peptone 0.5%-2%, biological nitrogen source 1%-5%, potassium dihydrogen phosphate 0.01%-0.1%, dipotassium hydrogen phosphate 0.1%-1%, magnesium sulfate 0.1%-1%, sulfur Acid manganese 0.001%-0.005%, surplus is water.
Enterococcus faecalis is inoculated in the culture medium of inventive formulation the process conditions carrying out fermenting: fermentation temperature 36-37 DEG C, micro-ventilation maintains tank pressure 0.03-0.04MPa, speed of agitator 80-100 rev/min, PH5.0-6.5, fermentation period 12 hours, can Obtain more than enterococcus faecalis fermentation liquid viable bacteria 15,000,000,000/milliliter.
Use conventional fermentation technology in the fermentation cylinder for fermentation of 500L, be up to 100 with plate dilution assay method viable count Hundred million cfu/ml, ferment 14-16 hour.
Embodiment 2
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 3%, peptone 1%, raw Thing nitrogen source 2%, potassium dihydrogen phosphate 0.05%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, manganese sulfate 0.002%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 3
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 1%, peptone 2%, raw Thing nitrogen source 1%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 1%, manganese sulfate 0.001%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 4
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 2%, peptone 1.8%, Biological nitrogen source 2%, potassium dihydrogen phosphate 0.08%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.9%, manganese sulfate 0.003%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 5
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 4%, peptone 1.6%, Biological nitrogen source 2.5%, potassium dihydrogen phosphate 0.07%, dipotassium hydrogen phosphate 0.3%, magnesium sulfate 0.8%, manganese sulfate 0.004%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 6
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 5%, peptone 0.5%, Biological nitrogen source 5%, potassium dihydrogen phosphate 0.01%, dipotassium hydrogen phosphate 1%, magnesium sulfate 0.1%, manganese sulfate 0.005%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 7
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 4.5%, peptone 1.2%, biological nitrogen source 4.5%, potassium dihydrogen phosphate 0.04%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 0.3%, manganese sulfate 0.004%, surplus For water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 8
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 4%, peptone 0.8%, Biological nitrogen source 3%, potassium dihydrogen phosphate 0.08%, dipotassium hydrogen phosphate 0.3%, magnesium sulfate 0.7%, manganese sulfate 0.003%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 9
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 2%, peptone 0.6%, Biological nitrogen source 4%, potassium dihydrogen phosphate 0.06%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.1%, manganese sulfate 0.002%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 10
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 5%, peptone 1.5%, Biological nitrogen source 2%, potassium dihydrogen phosphate 0.08%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.9%, manganese sulfate 0.001%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 11
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 3%, peptone 0.6%, Biological nitrogen source 4%, potassium dihydrogen phosphate 0.08%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.2%, manganese sulfate 0.004%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 12
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 2%, peptone 2%, raw Thing nitrogen source 4%, potassium dihydrogen phosphate 0.04%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.8%, manganese sulfate 0.003%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 13
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 3%, peptone 1.5%, Biological nitrogen source 2%, potassium dihydrogen phosphate 0.03%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 0.7%, manganese sulfate 0.002%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 14
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 2%, peptone 0.5%, Biological nitrogen source 4%, potassium dihydrogen phosphate 0.06%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.8%, manganese sulfate 0.003%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 15
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 5%, peptone 1.5%, Biological nitrogen source 3%, potassium dihydrogen phosphate 0.09%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 0.3%, manganese sulfate 0.001%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 16
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 3.5%, peptone 2%, Biological nitrogen source 2%, potassium dihydrogen phosphate 0.04%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, manganese sulfate 0.002%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 17
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 2.5%, peptone 2%, Biological nitrogen source 4.5%, potassium dihydrogen phosphate 0.07%, dipotassium hydrogen phosphate 0.8%, magnesium sulfate 0.3%, manganese sulfate 0.004%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 18
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 4%, peptone 1%, raw Thing nitrogen source 1%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.7%, manganese sulfate 0.003%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 19
A kind of enterococcus faecalis fermentation medium, it is made up of the component of following mass percent: sucrose 5%, peptone 0.5%, Biological nitrogen source 1%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 1%, magnesium sulfate 0.1%, manganese sulfate 0.003%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
The product that embodiment 2 ~ 19 cultivation obtains is for detecting, and testing result is as follows:

Claims (6)

1. an enterococcus faecalis fermentation medium, it is characterised in that it is made up of the component of following mass percent: sucrose 1%- 5%, peptone 0.5%-2%, biological nitrogen source 1%-5%, potassium dihydrogen phosphate 0.01%-0.1%, dipotassium hydrogen phosphate 0.1%-1%, magnesium sulfate 0.1%-1%, manganese sulfate 0.001%-0.005%, surplus is water.
Enterococcus faecalis fermentation medium the most according to claim 1, it is characterised in that it is by following mass percent Component forms: sucrose 1%-3%, peptone 1.5%-2%, biological nitrogen source 1%-3%, potassium dihydrogen phosphate 0.01%-0.04%, phosphoric acid hydrogen two Potassium 0.6%-1%, magnesium sulfate 0.4%-1%, manganese sulfate 0.004%-0.005%, surplus is water.
Enterococcus faecalis fermentation medium the most according to claim 1, it is characterised in that it is by following mass percent Component forms: sucrose 3.1%-5%, peptone 0.5%-1.4%, biological nitrogen source 3.1%-5%, potassium dihydrogen phosphate 0.05%-0.1%, phosphorus Acid hydrogen dipotassium 0.1%-0.55%, magnesium sulfate 0.1%-0.35%, manganese sulfate 0.001%-0.003%, surplus is water.
Enterococcus faecalis fermentation medium the most according to claim 1, it is characterised in that it is by following mass percent Component forms: sucrose 3%, peptone 1%, biological nitrogen source 2%, potassium dihydrogen phosphate 0.05%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, manganese sulfate 0.002%, surplus is water.
Enterococcus faecalis fermentation medium the most according to claim 1, it is characterised in that: described biological nitrogen source is mainly by amino Acid, vitamin and mineral composition.
Enterococcus faecalis fermentation medium the most according to claim 1, it is characterised in that: dry in the composition in described biological nitrogen source Material is more than 70%, and total nitrogen is more than 8%, and amino-acid nitrogen is more than 2%, and ash is less than 15%, pH value 5.0-6.5.
CN201610760483.4A 2016-08-30 2016-08-30 A kind of enterococcus faecalis fermentation medium Pending CN106190926A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115745674A (en) * 2022-11-04 2023-03-07 江西农业大学 Microbial oligosaccharide chelated trace element water-soluble fertilizer and preparation method thereof

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CN104560820A (en) * 2014-12-30 2015-04-29 杭州师范大学 Enterococcus faecium KQ2.6 and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115745674A (en) * 2022-11-04 2023-03-07 江西农业大学 Microbial oligosaccharide chelated trace element water-soluble fertilizer and preparation method thereof

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