CN106148245A - A kind of fermentation of bacillus subtilis culture medium - Google Patents
A kind of fermentation of bacillus subtilis culture medium Download PDFInfo
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- CN106148245A CN106148245A CN201610760481.5A CN201610760481A CN106148245A CN 106148245 A CN106148245 A CN 106148245A CN 201610760481 A CN201610760481 A CN 201610760481A CN 106148245 A CN106148245 A CN 106148245A
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Abstract
The present invention relates to a kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 0.5% 2%, peptone 1% 3%, biological nitrogen source 1% 5%, sucrose 0.5% 2%, magnesium sulfate 0.01% 0.1%, manganese sulfate 0.1% 1%, dipotassium hydrogen phosphate 0.1% 1%, potassium dihydrogen phosphate 0.01% 0.1%, surplus is water.This culture medium can promote the good growth of bacillus subtilis, improves the product spore rate of bacillus subtilis, low cost.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to a kind of fermentation of bacillus subtilis culture medium.
Background technology
Bacillus subtilis is the one of bacillus, belongs to gram positive bacteria, without pod membrane, peritrichous, can transport
Dynamic.Bacillus subtilis as probiotic bacteria, have the advantage that during thalli growth produce subtilin, polymyxin,
Nystatin, Gramicidin isoreactivity material, the conditioned pathogen of pathogenic bacterium or autogenous infection is had bright by these active substances
Aobvious inhibitory action；The free oxygen in environment can be consumed rapidly, cause intestinal hypoxia, promote that useful anaerobe grows, and produce
Organic acid, reduces gut pH, indirectly suppresses the growth of other pathogenic bacterium；The growth promoter of stimulating animal immune organ, activates T, B
Lymphocyte, improves immunoglobulin and antibody horizontal, strengthens cellular immunization and humoral immune function, improves herd immunity；
Thalline self can synthesize the enzyme elements such as α-amylase, protease, lipase, cellulase, with disappearing in animal body in digestive tract
Change enzyme together to play a role；The multiple vitamin B group such as energy synthetic vitamin B1, B2, B6, nicotinic acid, improve animal body internal interference element
Activity with macrophage.Bacillus subtilis is as useful microorganism, at present at purifying aquatic product cultivating environment, crop disease
The aspects such as evil preventing and treating, additive for farm animal feed achieve well applies effect, the most increasingly by the extensive pass of people
Note.
But, in current industrial processes, bacillus subtilis microbial agent exists that spore forming rate is the highest, produces into
This too high problem；In microbial inoculum, spore rate is low, and the thalline not having spore can be dead at short notice, is substantially reduced the activity of microbial inoculum
And quality stability.
Summary of the invention
It is an object of the invention to provide a kind of fermentation of bacillus subtilis culture medium, this culture medium can promote hay spore
The good growth of bacillus, improves the product spore rate of bacillus subtilis, low cost.
For achieving the above object, the technical solution used in the present invention is as follows:
The invention provides a kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: beautiful
Rice flour 0.5%-2%, peptone 1%-3%, biological nitrogen source 1%-5%, sucrose 0.5%-2%, magnesium sulfate 0.01%-0.1%, manganese sulfate
0.1%-1%, dipotassium hydrogen phosphate 0.1%-1%, potassium dihydrogen phosphate 0.01%-0.1%, surplus is water.
According to above-mentioned fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis
Powder 0.5%-1%, peptone 2.2%-3%, biological nitrogen source 3.1%-5%, sucrose 0.5%-1.2%, magnesium sulfate 0.01%-0.06%, sulphuric acid
Manganese 0.1%-0.5%, dipotassium hydrogen phosphate 0.5%-1%, potassium dihydrogen phosphate 0.01%-0.05%, surplus is water.
According to above-mentioned fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis
Powder 1.1%-2%, peptone 1%-2.1%, biological nitrogen source 1%-3%, sucrose 1.3%-2%, magnesium sulfate 0.07%-0.1%, manganese sulfate
0.6%-1%, dipotassium hydrogen phosphate 0.1%-0.4%, potassium dihydrogen phosphate 0.06%-0.1%, surplus is water.
According to above-mentioned fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis
Powder 1%, peptone 2%, biological nitrogen source 2.5%, sucrose 1%, magnesium sulfate 0.05%, manganese sulfate 0.4%, dipotassium hydrogen phosphate 0.5%, phosphoric acid
Potassium dihydrogen 0.05%, surplus is water.
Described biological nitrogen source is mainly made up of aminoacid, vitamin and mineral.
In the composition in described biological nitrogen source, dry is more than 70%, and total nitrogen is more than 8%, and amino-acid nitrogen is more than 2%, and ash is little
In 15%, pH value 5.0-6.5.
Compared with prior art, beneficial effects of the present invention:
The present invention uses Semen Maydis powder, peptone, biological nitrogen source, sucrose, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and biphosphate
Potassium is configured to fermentation medium according to a certain percentage, can promote the good growth of bacillus subtilis, improve bacillus subtilis
Product spore rate；The bacillus subtilis microbial agent using this culture medium culturing has higher spore rate, it is ensured that the work of microbial inoculum
Property, improve quality and the quality stability of product；Meanwhile, the bacillus subtilis microbial agent using this culture medium culturing has and carries
High immunity and the effect of promotion feed intake.
Detailed description of the invention
Following example are intended to further illustrate present disclosure, but are not intended to protection scope of the present invention.
Following raw material corn powder, peptone, biological nitrogen source, sucrose, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and di(2-ethylhexyl)phosphate
Hydrogen potassium is commercially available；The producer in biological nitrogen source be Xuzhou City be a day Science and Technology Ltd..
Embodiment 1
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 0.5%-2%,
Peptone 1%-3%, biological nitrogen source 1%-5%, sucrose 0.5%-2%, magnesium sulfate 0.01%-0.1%, manganese sulfate 0.1%-1%, phosphoric acid hydrogen two
Potassium 0.1%-1%, potassium dihydrogen phosphate 0.01%-0.1%, surplus is water.
Use conventional fermentation technology that bacillus subtilis is cultivated, technological condition for fermentation: temperature: 35-37 DEG C is logical
Gas 1:1-1.2, rotating speed: 120-150 rev/min, pH value 6.5-7.8.
Embodiment 2
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1%, peptone
2%, biological nitrogen source 2.5%, sucrose 1%, magnesium sulfate 0.05%, manganese sulfate 0.4%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.05%,
Surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 3
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 0.5%, albumen
Peptone 3%, biological nitrogen source 1%, sucrose 2%, magnesium sulfate 0.01%, manganese sulfate 1%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%, remaining
Amount is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 4
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 0.8%, albumen
Peptone 2.8%, biological nitrogen source 1.5%, sucrose 1.8%, magnesium sulfate 0.02%, manganese sulfate 0.9%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate
0.09%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 5
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1%, peptone
2.6%, biological nitrogen source 1.8%, sucrose 1.6%, magnesium sulfate 0.03%, manganese sulfate 0.8%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate
0.08%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 6
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.3%, albumen
Peptone 2.4%, biological nitrogen source 2%, sucrose 1.5%, magnesium sulfate 0.04%, manganese sulfate 0.7%, dipotassium hydrogen phosphate 0.4%, potassium dihydrogen phosphate
0.07%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 7
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.5%, albumen
Peptone 2.2%, biological nitrogen source 2.2%, sucrose 1.4%, magnesium sulfate 0.05%, manganese sulfate 0.6%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate
0.06%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 8
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.6%, albumen
Peptone 2%, biological nitrogen source 2.4%, sucrose 1.2%, magnesium sulfate 0.06%, manganese sulfate 0.5%, dipotassium hydrogen phosphate 0.6%, potassium dihydrogen phosphate
0.05%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 9
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.8%, albumen
Peptone 1.8%, biological nitrogen source 2.8%, sucrose 1%, magnesium sulfate 0.07%, manganese sulfate 0.4%, dipotassium hydrogen phosphate 0.7%, potassium dihydrogen phosphate
0.04%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 10
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 2%, peptone
1%, biological nitrogen source 5%, sucrose 0.5%, magnesium sulfate 0.1%, manganese sulfate 0.1%, dipotassium hydrogen phosphate 1%, potassium dihydrogen phosphate 0.01%, remaining
Amount is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 11
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.6%, albumen
Peptone 2.1%, biological nitrogen source 3.5%, sucrose 0.5%, magnesium sulfate 0.03%, manganese sulfate 0.4%, dipotassium hydrogen phosphate 0.6%, potassium dihydrogen phosphate
0.07%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 12
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.3%, albumen
Peptone 1.9%, biological nitrogen source 2.9%, sucrose 0.6%, magnesium sulfate 0.05%, manganese sulfate 0.9%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate
0.06, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 13
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.6%, albumen
Peptone 1.4%, biological nitrogen source 2.2%, sucrose 0.7%, magnesium sulfate 0.06%, manganese sulfate 0.3%, dipotassium hydrogen phosphate 0.8%, potassium dihydrogen phosphate
0.09%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 14
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 0.8%, albumen
Peptone 2.2%, biological nitrogen source 3.2%, sucrose 1%, magnesium sulfate 0.06%, manganese sulfate 0.2%, dipotassium hydrogen phosphate 0.7%, potassium dihydrogen phosphate
0.05%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 15
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.1%, albumen
Peptone 1.9%, biological nitrogen source 2.3%, sucrose 0.9%, magnesium sulfate 0.1%, manganese sulfate 0.3%, dipotassium hydrogen phosphate 0.4%, potassium dihydrogen phosphate
0.06%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 16
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.5%, albumen
Peptone 2.5%, biological nitrogen source 2%, sucrose 1.4%, magnesium sulfate 0.09%, manganese sulfate 0.3%, dipotassium hydrogen phosphate 0.4%, potassium dihydrogen phosphate
0.06%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 17
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 2%, peptone
1%, biological nitrogen source 3%, sucrose 1%, magnesium sulfate 0.03%, manganese sulfate 0.8%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.04%, remaining
Amount is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 18
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 1.2%, albumen
Peptone 1.1%, biological nitrogen source 2%, sucrose 0.9%, magnesium sulfate 0.06%, manganese sulfate 0.8%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate
0.02%, surplus is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Embodiment 19
A kind of fermentation of bacillus subtilis culture medium, it is made up of the component of following mass percent: Semen Maydis powder 2%, peptone
2%, biological nitrogen source 3%, sucrose 1%, magnesium sulfate 0.04%, manganese sulfate 0.6%, dipotassium hydrogen phosphate 0.9%, potassium dihydrogen phosphate 0.06%, remaining
Amount is water.
The technological condition for fermentation of the present embodiment is same as in Example 1.
Bacillus subtilis is cultivated by the fermentation of bacillus subtilis culture medium using embodiment 2～19, its result
As shown in table 1:
Claims (6)
1. a fermentation of bacillus subtilis culture medium, it is characterised in that it is made up of the component of following mass percent: beautiful
Rice flour 0.5%-2%, peptone 1%-3%, biological nitrogen source 1%-5%, sucrose 0.5%-2%, magnesium sulfate 0.01%-0.1%, manganese sulfate
0.1%-1%, dipotassium hydrogen phosphate 0.1%-1%, potassium dihydrogen phosphate 0.01%-0.1%, surplus is water.
Fermentation of bacillus subtilis culture medium the most according to claim 1, it is characterised in that it is by following percent mass
The component composition of ratio: Semen Maydis powder 0.5%-1%, peptone 2.2%-3%, biological nitrogen source 3.1%-5%, sucrose 0.5%-1.2%, magnesium sulfate
0.01%-0.06%, manganese sulfate 0.1%-0.5%, dipotassium hydrogen phosphate 0.5%-1%, potassium dihydrogen phosphate 0.01%-0.05%, surplus is water.
Fermentation of bacillus subtilis culture medium the most according to claim 1, it is characterised in that it is by following percent mass
The component composition of ratio: Semen Maydis powder 1.1%-2%, peptone 1%-2.1%, biological nitrogen source 1%-3%, sucrose 1.3%-2%, magnesium sulfate
0.07%-0.1%, manganese sulfate 0.6%-1%, dipotassium hydrogen phosphate 0.1%-0.4%, potassium dihydrogen phosphate 0.06%-0.1%, surplus is water.
Fermentation of bacillus subtilis culture medium the most according to claim 1, it is characterised in that it is by following percent mass
The component composition of ratio: Semen Maydis powder 1%, peptone 2%, biological nitrogen source 2.5%, sucrose 1%, magnesium sulfate 0.05%, manganese sulfate 0.4%, phosphorus
Acid hydrogen dipotassium 0.5%, potassium dihydrogen phosphate 0.05%, surplus is water.
Fermentation of bacillus subtilis culture medium the most according to claim 1, it is characterised in that: described biological nitrogen source mainly by
Aminoacid, vitamin and mineral composition.
Fermentation of bacillus subtilis culture medium the most according to claim 1, it is characterised in that: the composition in described biological nitrogen source
Middle dry is more than 70%, and total nitrogen is more than 8%, and amino-acid nitrogen is more than 2%, and ash is less than 15%, pH value 5.0-6.5.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107254425A (en) * | 2017-08-04 | 2017-10-17 | 河南振新生物技术股份有限公司 | A kind of bacillus subtilis bacterium culture medium and preparation method thereof |
| CN110272931A (en) * | 2019-05-31 | 2019-09-24 | 贵州医科大学 | A kind of culture medium and its cultural method promoting the synthesis of antibacterial polypeptide gramicidins |
| CN113717894A (en) * | 2021-09-22 | 2021-11-30 | 武汉华扬天乐生物科技有限公司 | Bacillus subtilis fermentation medium and preparation method and application thereof |
| CN118308278A (en) * | 2024-06-13 | 2024-07-09 | 珠海鱼来鱼旺生物科技有限公司 | High-density liquid fermentation medium of bacillus subtilis and fermentation method |
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| CN103146617A (en) * | 2013-03-18 | 2013-06-12 | 济南巨微生物技术有限公司 | Bacillus subtilis and culture method and application thereof |
| CN103911396A (en) * | 2014-04-25 | 2014-07-09 | 山东仙普爱瑞科技股份有限公司 | Bacillus subtilis fermentation medium |
| CN105432667A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Preparation method of bacillus subtilis wettable powder |
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- 2016-08-30 CN CN201610760481.5A patent/CN106148245A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103146617A (en) * | 2013-03-18 | 2013-06-12 | 济南巨微生物技术有限公司 | Bacillus subtilis and culture method and application thereof |
| CN103911396A (en) * | 2014-04-25 | 2014-07-09 | 山东仙普爱瑞科技股份有限公司 | Bacillus subtilis fermentation medium |
| CN105432667A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Preparation method of bacillus subtilis wettable powder |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107254425A (en) * | 2017-08-04 | 2017-10-17 | 河南振新生物技术股份有限公司 | A kind of bacillus subtilis bacterium culture medium and preparation method thereof |
| CN110272931A (en) * | 2019-05-31 | 2019-09-24 | 贵州医科大学 | A kind of culture medium and its cultural method promoting the synthesis of antibacterial polypeptide gramicidins |
| CN113717894A (en) * | 2021-09-22 | 2021-11-30 | 武汉华扬天乐生物科技有限公司 | Bacillus subtilis fermentation medium and preparation method and application thereof |
| CN118308278A (en) * | 2024-06-13 | 2024-07-09 | 珠海鱼来鱼旺生物科技有限公司 | High-density liquid fermentation medium of bacillus subtilis and fermentation method |
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Application publication date: 20161123 |