CN106190915A - 一种携氧载体粉末在培养需氧菌中的应用 - Google Patents
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Abstract
一种携氧载体粉末在培养需氧菌中的应用，将携氧载体粉末均匀加入到微生物培养基中，再接种需氧菌，即可实现需氧菌的快速培养；所述的携氧载体粉末由低表能固体材料、载体材料组成。本发明提供的携氧载体粉末具有亲氧、耐紫外线、耐氧化、耐微生物分解等优良性质，将其添加到微生物培养基中，可以使需氧菌在氧气充足的条件下，可以大量繁殖、生长，且本发明得到的需氧菌数量是普通培养基的数十倍以上。
Description
技术领域
本发明属于微生物领域，具体涉及一种携氧载体粉末在培养需氧菌中的应用。
背景技术
葡萄是世界五大果树树种之一，葡萄是一种世界性水果，在世界五大果树种中居第二位。我国也是葡萄栽培的主产区果树生产中也占有很重要的位置，2003年我国葡萄栽培421千公顷，产量5,175,939吨(中国年鉴2003)。葡萄中除富含多种维生素、氨基酸、矿物质、葡萄糖及果糖等营养成分外，还含有白藜芦醇、齐墩果酸、β-谷甾醇、黄酮、鞣质、SOD等多种活性物质，具有改善心脑血管循环、抗氧化、抗衰老、抗病毒、抗菌和增强免疫等多方面的医疗保健功能。葡萄用途广泛，既是人们喜爱并长期食用的果品，酿造不同类型的葡萄酒，加工成其他产品：葡萄干、葡萄汁、葡萄罐头。
但是，近年来随着产量的提高。病虫害必有加重的趋势，尤其是真菌病害，在多雨的季节，一旦气候适宜，大面积曼延，控制起来相当困难一旦发病很难治愈。为防治葡萄病害，农民一般多采用增加喷药次数或加大药液浓度，这样不但会增加农药在农产品中的残留，也会使病害对化学药剂产生抵抗作用。使病害加重造成葡萄品质和产量下降。为增加产量，农民大量施用化肥，这样不但破坏了土壤结构并且影响了果实的品质和风味，还达不到增产的效果，有时病害严重时
会造成绝收。施用农药和化肥增大了生产成本，降低了农民的收入。过量施用化肥和农药会对生态环境造成污染，不但影响我们的健康，还会影响到子孙后代。随着人民生活水平提高，消费观念发生了变化，追求健康、绿色食品成为人们的消费时尚。如何解决农民要增产又要降低化学农药施用量的矛盾，开发新的生物菌、化肥来实现葡萄的优质、高产栽培是一个较好的途径。
发明内容
本发明为了克服现有技术的不足，提供了一种携氧载体粉末在培养需氧菌中的应用。
本发明可通过以下技术方案来实现：
一种携氧载体粉末在培养需氧菌中的应用，主要将携氧载体粉末均匀加入到微生物培养基中，再接种需氧菌，即可实现需氧菌的快速培养；所述的携氧载体粉末由低表能固体材料、载体材料组成。
进一步地，所述的低表能固体材料为氟碳树脂、聚六氟丙烯、聚四氟乙烯、聚全氟乙丙烯(F46)、聚三氟乙烯、聚偏氟乙稀、聚氟乙烯、聚三氟氯乙烯、三氟氯乙烯中的任一种或数种。
进一步地，所述的载体材料为石英沙、火山岩粉末、活性炭粉末中的任一种。
进一步地，所述的需氧菌为枯草芽孢杆菌、内生芽孢杆菌、哈茨木霉菌、硝化细菌、圆褐固氮菌。
进一步地，所述的携氧载体粉末的制备方法如下：
(1)称取2-12重量份的低表能固体材料，将其磨成细度不大于100目的粉末，分散在10-50重量份的水中，形成分散液；
(2)称取1-10重量份的载体材料，将步骤1中的分散液均匀喷洒在载体材料表面，风干处理后再用粉碎机粉碎，得到的混合粉料即为携氧载体粉末。
本发明的技术特点和效果为：携氧载体培养基中加入低表能固体材料和载体材料，通过将其粉碎成粉末，增大彼此的接触面积；同时携氧载体粉末具有亲氧、耐紫外线、耐氧化、耐微生物分解等优良性质，可以使需氧菌在氧气充足的条件下，可以大量繁殖、生长。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
实施例
一种携氧载体粉末在培养需氧菌中的应用，主要将携氧载体粉末均匀加入到微生物培养基中，再接种需氧菌，即可实现需氧菌的快速培养；所述的携氧载体粉末由低表能固体材料、载体材料组成。
其制备方法包括：(1)称取2重量份的氟碳树脂低表能固体材料，将其磨成细度不大于100目的粉末，分散在20重量份的水中，形成分散液；(2)称取2重量份的载体材料，将步骤1中的分散液均匀喷洒在石英沙表面，风干处理后再用粉碎机粉碎，得到的混合粉料即为携氧载体粉末；(3)将步骤2中得到的携氧载体粉末均匀的添加到微生物培养基内，升高温度至95℃进行杀菌处理，时间为15min；(4)向步骤3杀菌后的培养基内接种枯草芽孢杆菌，再于37℃恒温培养24h。
将上述制备方法与普通培养基培养的枯草芽孢杆菌相比，在其他条件相同的情况下，本发明提供的方法得到的枯草芽孢杆菌数量是普通培养基的50倍。
Claims (1)
1.一种携氧载体粉末在培养需氧菌中的应用。
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105621642A (zh) * | 2016-02-03 | 2016-06-01 | 安徽乙地生态科技有限公司 | 一种肺式曝气在污水处理工艺中的应用 |
| CN105643857A (zh) * | 2016-02-03 | 2016-06-08 | 安徽乙地生态科技有限公司 | 污水处理中用于提高曝气效率的肺式曝气海绵 |
| CN105668790A (zh) * | 2016-02-03 | 2016-06-15 | 安徽乙地生态科技有限公司 | 污水处理工艺中提高曝气效率肺式曝气海绵 |
| CN105731631A (zh) * | 2016-02-03 | 2016-07-06 | 安徽乙地生态科技有限公司 | 污水处理工艺中利于提高曝气效率的肺式曝气海绵 |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105621642A (zh) * | 2016-02-03 | 2016-06-01 | 安徽乙地生态科技有限公司 | 一种肺式曝气在污水处理工艺中的应用 |
| CN105643857A (zh) * | 2016-02-03 | 2016-06-08 | 安徽乙地生态科技有限公司 | 污水处理中用于提高曝气效率的肺式曝气海绵 |
| CN105668790A (zh) * | 2016-02-03 | 2016-06-15 | 安徽乙地生态科技有限公司 | 污水处理工艺中提高曝气效率肺式曝气海绵 |
| CN105731631A (zh) * | 2016-02-03 | 2016-07-06 | 安徽乙地生态科技有限公司 | 污水处理工艺中利于提高曝气效率的肺式曝气海绵 |
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