CN106188042A - Use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method - Google Patents
Use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method Download PDFInfo
- Publication number
- CN106188042A CN106188042A CN201610515247.6A CN201610515247A CN106188042A CN 106188042 A CN106188042 A CN 106188042A CN 201610515247 A CN201610515247 A CN 201610515247A CN 106188042 A CN106188042 A CN 106188042A
- Authority
- CN
- China
- Prior art keywords
- pyrroloquinoline quinone
- fermentation liquid
- phase extraction
- solid phase
- molecular engram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/04—Ortho-condensed systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/268—Polymers created by use of a template, e.g. molecularly imprinted polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses and a kind of use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, the method mainly utilizes pyrroloquinoline quinone molecularly imprinted polymer to obtain pyrroloquinoline quinone from rich in isolated and purified pyrroloquinoline quinone fermentation liquid.The present invention is with silicon dioxide as base material, and pyrroloquinoline quinone is template molecule, and methacrylic acid is function monomer, and ethylene glycol dimethacrylate is cross-linking agent, uses thermal polymerization technology to prepare pyrroloquinoline quinone molecularly imprinted polymer.Absorption property experiment shows, pyrroloquinoline quinone molecularly imprinted polymer prepared by the present invention has the most special, to select adsorbed target thing pyrroloquinoline quinone performance, and maximum adsorption capacity is up to 10mg/mg (molecularly imprinted polymer).For solid phase extraction filler, target product pyrroloquinoline quinone is carried out Solid-Phase Extraction with this polymer, it is achieved that the purpose of high efficiency separation purification pyrroloquinoline quinone from fermentation liquid.
Description
Technical field
The present invention relates to a kind of use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method,
Belong to technical field of biochemical industry.
Background technology
Pyrroloquinoline quinone (PQQ) is a kind of water solublity quinones, is widely present in food and animal, is glucose
Dehydrogenase and the coenzyme of ethanol dehydrogenase.Owing to PQQ has critically important physiological function, therefore PQQ is also considered as a kind of new
Vitamin B group.Research shows, PQQ can stop the formation of amyloid, the anomaly synapse core egg that suppression C-end shortens
White cytotoxicity, therefore PQQ may be used for nerve and the treatment of moral ataxia disease.It addition, PQQ can also effectively stop
The nerve retrograde affection that oxidative stress causes.In simple terms, relevant to nervous system for PQQ function has following four kinds: (1) resists
Oxidation, removes free radical；(2) affect respiratory chain function, safeguard mitochondrial；(3) dividing of nerve growth factor is stimulated
Secrete, repair and promote nerve growth；(4) delay the deposition of α-synuclein albumen, prevent neurocyte fibrosis.Therefore, have
Guan research worker thinks that PQQ neurodegenerative disease multiple to parkinson disease and alzheimer disease etc. has potential treatment valency
Value.So, the method finding simple acquisition PQQ, carry out industrialization production and be significant.
The most conventional PQQ synthetic method is mainly chemical method synthesis, but chemosynthesis PQQ step is many, and productivity is low, different
The removal of structure body and side-product needs more purification, and needs to use multiple toxic reagent, pollutes environment.Therefore, research is such as
What utilizes non-chemical method acquisition PQQ to have more industrialization meaning.PQQ is widely present in gram-negative bacteria, but synthetic quantity
Different, some bacterium only produces trace PQQ, for normal physiological metabolism demand, such as pseudomonas putida；Also some bacterium but can
Produce the PQQ of excess, and be secreted into outside born of the same parents.The wild mushroom that can produce excess PQQ found so far includes bacillus of oxidizing glucose
Belong to (Gluconobacter), achromobacter (Achromobacter), Methanomonas (Methanomonas), methyl bacterium
Belong to (Methy-lobacillus), methylomonas (Methylomonas), addicted to Methylobacillus (Methy-lophilus) and
Flavobacterium (Xanthobacter) etc..At present, the method using fermentable has been had to produce the correlational study of PQQ,
The original strain PQQ yield used is at 0.07-7mg/L, and fermentation time is about 2-5 days, but in these researchs not
Simple isolated and purified PQQ from microbial fermentation solution is provided, makes the preparation of PQQ realize the side of industrial-scale production
Method.
Molecularly imprinted polymer (MIPs) is a kind of polymer to target molecule with specific absorbability.From 1972
Since Wulff etc. successfully prepare MIPs, engram technology research and development is rapid.MIPs is in chromatographic stationary phases, solid phase extraction at present
Taking, is used widely in sensor, membrance separation, the field such as enzyme catalysis.Using MIPs as solid phase extraction filler, extraction can be strengthened
Selectivity and adsorption capacity, there is good application prospect.
Summary of the invention
The technical problem to be solved is to provide a kind of employing isolated and purified fermentation of molecular engram solid phase extraction method
The method of pyrroloquinoline quinone in liquid, the method can isolated and purified pyrroloquinoline quinone simple, efficient, extensive, be more suitable for pyrroles
The industrialized development of quinoline quinone.
To achieve these goals, the technical solution adopted in the present invention is:
Use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, comprise the following steps:
(1) prepare: utilize pyrroloquinoline quinone to produce the bacterium preparation fermentation liquid containing pyrroloquinoline quinone；
(2) extract and separate: use molecular engram solid phase extraction method to extract the fermentation liquid containing pyrroloquinoline quinone, will
Fermentation liquid containing pyrroloquinoline quinone is splined in the molecular engram extraction column being filled with molecularly imprinted polymer, uses the most successively
Ethanol and ethanol-organic acid mixed liquor eluting, collect ethanol-organic acid mixing eluent, concentrating under reduced pressure, obtain pyrroloquinoline quinone
Crude product；
(3) purification: pyrroloquinoline quinone crude product ultra-pure water is dissolved, regulates pH to 3-4, add ethanol, stand after stirring,
Obtain pyrroloquinoline quinone.
Described molecularly imprinted polymer is with silicon dioxide as base material, and pyrroloquinoline quinone is template molecule, metering system
Acid is function monomer, and ethylene glycol dimethacrylate is cross-linking agent, uses thermal polymerization technology to be prepared from.
The preparation method of described molecularly imprinted polymer is: by 100-300mg pyrroloquinoline quinone, 0.1-0.5mL methyl
Acrylic acid, joins in 50-200mL acetonitrile solution, room temperature mechanical stirring 6-20h；It is subsequently adding 100-800mg silicon dioxide micro-
Ball, ultrasound wave disperses；Add 0.6-1.0mL ethylene glycol dimethacrylate and 50-150mg azodiisobutyronitrile, be passed through
Nitrogen 5min, seals, is heated to 50-70 DEG C, and mechanic whirl-nett reaction 8-20h separates, obtains polymeric material；Polymeric material is used
After washing with alcohol 5-10 time, 50 DEG C of vacuum drying 8-16h；Do with volume ratio 6-10:1 ethanol/acetic acid mixed solution eluting repeatedly
Polymeric material after dry, removing the template molecule pyrroloquinoline quinone in polymeric material, dividing until can't detect template in eluent
Sub-pyrroloquinoline quinone, finally with the polymeric material after distilled water wash eluting to neutral, 50 DEG C of vacuum drying 12h, obtain molecule
Imprinted polymer, standby.
The particle diameter of described silicon dioxide microsphere is 100-200 mesh.
Described molecularly imprinted polymer and solid-to-liquid ratio 1:100-500 (g/ml) of the fermentation liquid containing pyrroloquinoline quinone.
In fermentation liquid containing pyrroloquinoline quinone, the amount of pyrroloquinoline quinone is with the maximum suction less than molecularly imprinted polymer
Attached amount is advisable.Preferably, in the described fermentation liquid containing pyrroloquinoline quinone, the concentration of pyrroloquinoline quinone is 100-200mg/L.
In described ethanol-organic acid mixed liquor, the volume ratio of ethanol and organic acid is 8-10:1-2；Described organic acid
For any one in formic acid, acetic acid or trifluoroacetic acid.
The vacuum of described concentrating under reduced pressure is 0.9MPa, and rotating speed is 150-180 revolutions per second.
In step (3), pyrroloquinoline quinone crude product ultra-pure water is dissolved to final concentration of 9-12g/L.
In step (3), ultra-pure water is 3-5:1 with the volume ratio of ethanol.
In step (3), regulation pH agents useful for same is hydrochloric acid.
In step (3), whipping temp is 20-25 DEG C, and mixing time is 5-6h, and time of repose is 12-24h.
Described pyrroloquinoline quinone produces bacterium and includes turning round demethylation bacillus (Methylobacterium extorquens),
Klebsiella pneumonia (Klebsiella Pneumonia), Gluconobacter oxvdans (Gluconobacter oxidans),
Bacillus pyocyaneus (Pseudomonas aeruginosa), streptomycete (Streptomyces rochei).
The principle of the method for molecular engram solid phase extraction separation pyrroloquinoline quinone of the present invention is shown in Fig. 1.
Beneficial effect
1, the present invention is with silicon dioxide as base material, and pyrroloquinoline quinone is template molecule, and methacrylic acid is function monomer, second
Diol dimethacrylate is cross-linking agent, uses thermal polymerization technology to prepare pyrroloquinoline quinone molecularly imprinted polymer.Should
Pyrroloquinoline quinone molecularly imprinted polymer has the most special, to select adsorbed target thing pyrroloquinoline quinone performance, maximum suction
Attached capacity is up to 10mg/mg (molecularly imprinted polymer).With this polymer for solid phase extraction filler to target product pyrroles's quinoline
Quinoline quinone carries out Solid-Phase Extraction, it is achieved that the purpose of high efficiency separation purification pyrroloquinoline quinone from fermentation liquid.
2, pyrroloquinoline quinone molecular engram solid phase extraction is a kind of new extract and separate system, and the present invention uses pyrroles's quinoline
Quinone molecule is template molecule, forms the polymer with pyrroloquinoline quinone molecular engram, and this polymer can be with selective absorption pyrrole
Cough up quinoline quinone, and do not adsorb other water-solubility impurities, thus efficiently, the isolated and purified pyrroloquinoline quinone of highly selective.Experiment table
Bright, the pyrroloquinoline quinone purity of isolated of the present invention is up to more than 99%, and the response rate is up to more than 90%.
3, the method technological operation of the present invention is simple, with low cost, it is simple to large-scale industrial production, to promoting pyrroles's quinoline
The industrialized development of quinoline quinone is significant.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the detailed description of the invention of the present invention is described in further detail.
Fig. 1 is the schematic diagram of the method for molecular engram solid phase extraction separation pyrroloquinoline quinone of the present invention.
Fig. 2 is the ultraviolet spectrogram of PQQ product of the present invention, and wherein, A is PQQ standard substance, and B is PQQ product of the present invention.
Fig. 3 is the mass spectrum of PQQ product of the present invention.
Fig. 4 is that the present invention extracts eluent HPLC chromatogram under 230nm.
Fig. 5 is PQQ product of the present invention HPLC chromatogram under 230nm.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.
Culture medium compound method used by the present invention is as follows:
Fermentation medium: containing 40g sorbitol, 20g yeast extract, 5g (NH in every liter of culture medium4)2SO4、2g
KH2PO4、5g MgSO4·H2O。
Enrichment medium: containing 80g sorbitol, 40g yeast extract, 10g (NH in every liter of culture medium4)2SO4、4g
KH2PO4、10g MgSO4·H2O。
Equipment used by concentrating under reduced pressure of the present invention is rotary evaporator.
Embodiment 1
A kind of use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, including following step
Rapid:
(1) prepare: utilize pyrroloquinoline quinone to produce the bacterium preparation fermentation liquid containing pyrroloquinoline quinone, method particularly includes: will
Gluconobacter oxvdans, according to the inoculum concentration of 5%, is inoculated in fermentation medium, and under the conditions of 28 DEG C, 3d is cultivated in concussion,
To seed liquor；Then by seed liquor according to the inoculum concentration of 10%, being inoculated in enrichment medium, under the conditions of 28 DEG C, concussion is cultivated
5d, culture fluid 5 DEG C, 9000r/min be centrifuged 15min, collect supernatant, and obtaining pyrroloquinoline quinone concentration is 100-200mg/L's
Fermentation liquid rich in pyrroloquinoline quinone；
(2) extract and separate: use molecular engram solid phase extraction method to extract the fermentation liquid containing pyrroloquinoline quinone, will
Fermentation liquid containing pyrroloquinoline quinone is splined in the molecular engram extraction column being filled with molecularly imprinted polymer, and molecular engram gathers
Compound is 1:100 (g/ml) with the solid-to-liquid ratio of the fermentation liquid containing pyrroloquinoline quinone, the most successively with the ethanol of 10 times of column volumes
With the ethanol-formic acid mixed liquor eluting of 10 times of column volumes, collect ethanol-formic acid mixing eluent, concentrating under reduced pressure, obtain pyrroles's quinoline
Quinoline quinone crude product；The vacuum of concentrating under reduced pressure is 0.9MPa, and rotating speed is 170 revolutions per seconds；
In described ethanol-formic acid mixed liquor, the volume ratio of ethanol and formic acid is 8:1；
The preparation method of described molecularly imprinted polymer is: by 100mg pyrroloquinoline quinone, 0.1mL methacrylic acid, add
Enter in 50mL acetonitrile solution, room temperature mechanical stirring 6h；Being subsequently adding 100mg silicon dioxide microsphere, ultrasound wave disperses；Add
0.6mL ethylene glycol dimethacrylate and 50mg azodiisobutyronitrile, be passed through nitrogen 5min, seals, is heated to 50 DEG C, machinery
Stirring reaction 20h, separates, obtains polymeric material；By polymeric material with after washing with alcohol 5 times, 50 DEG C are vacuum dried 8h；Use volume
Ratio 6:1 ethanol/acetic acid mixed solution dried polymeric material of eluting repeatedly, removes the template molecule pyrroles's quinoline in polymeric material
Quinoline quinone, until can't detect template molecule pyrroloquinoline quinone, finally with the polymeric material after distilled water wash eluting in eluent
To neutral, it be dried 12h under 50 DEG C of vacuum, obtain molecularly imprinted polymer, standby；
The particle diameter of described silicon dioxide microsphere is 100-200 mesh；
(3) purification: pyrroloquinoline quinone crude product ultra-pure water is dissolved to the final concentration of 9g/L of pyrroloquinoline quinone, adds hydrochloric acid
Regulation pH to 3, adds ethanol, and ultra-pure water is 3:1 with the volume ratio of ethanol, stands 24h, obtain under the conditions of 20 DEG C after stirring 5h
The crystal of pyrroloquinoline quinone.
Embodiment 2
A kind of use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, including following step
Rapid:
(1) prepare: utilize pyrroloquinoline quinone to produce the bacterium preparation fermentation liquid containing pyrroloquinoline quinone, method particularly includes: will
Gluconobacter oxvdans, according to the inoculum concentration of 5%, is inoculated in fermentation medium, and under the conditions of 28 DEG C, 3d is cultivated in concussion,
To seed liquor；Then by seed liquor according to the inoculum concentration of 10%, being inoculated in enrichment medium, under the conditions of 28 DEG C, concussion is cultivated
5d, culture fluid 5 DEG C, 9000r/min be centrifuged 15min, collect supernatant, and obtaining pyrroloquinoline quinone concentration is 100-200mg/L's
Fermentation liquid rich in pyrroloquinoline quinone；
(2) extract and separate: use molecular engram solid phase extraction method to extract the fermentation liquid containing pyrroloquinoline quinone, will
Fermentation liquid containing pyrroloquinoline quinone is splined in the molecular engram extraction column being filled with molecularly imprinted polymer, and molecular engram gathers
Solid-to-liquid ratio 1:200 (g/ml) of compound and fermentation liquid containing pyrroloquinoline quinone, the most successively with the ethanol of 10 times of column volumes with
The Ethanol-Acetic Acid mixed liquor eluting of 10 times of column volumes, collects Ethanol-Acetic Acid mixing eluent, concentrating under reduced pressure, obtains pyrroles's quinoline
Quinone crude product；The vacuum of concentrating under reduced pressure is 0.9MPa, and rotating speed is 160 revolutions per seconds；
In described Ethanol-Acetic Acid mixed liquor, the volume ratio of ethanol and acetic acid is 10:2；
The preparation method of described molecularly imprinted polymer is: by 200mg pyrroloquinoline quinone, 0.3mL methacrylic acid, add
Enter in 100mL acetonitrile solution, room temperature mechanical stirring 10h；Being subsequently adding 300mg silicon dioxide microsphere, ultrasound wave disperses；Add again
Enter 0.8mL ethylene glycol dimethacrylate and 80mg azodiisobutyronitrile, be passed through nitrogen 5min, seal, be heated to 60 DEG C, machine
Tool stirring reaction 10h, separates, obtains polymeric material；By polymeric material with after washing with alcohol 8 times, 50 DEG C are vacuum dried 10h；With
Volume ratio 8:1 ethanol/dried polymeric material of acetic acid mixed solution eluting repeatedly, removes the template molecule pyrrole in polymeric material
Cough up quinoline quinone, until eluent can't detect template molecule pyrroloquinoline quinone, finally with the polymerization after distilled water wash eluting
Material, to neutral, be dried 12h, obtain molecularly imprinted polymer under 50 DEG C of vacuum, standby；
The particle diameter of described silicon dioxide microsphere is 100-200 mesh；
(3) purification: pyrroloquinoline quinone crude product ultra-pure water is dissolved to the final concentration of 10g/L of pyrroloquinoline quinone, adds salt
Acid for adjusting pH, to 4, adds ethanol, and ultra-pure water is 3:1 with the volume ratio of ethanol, stands 20h after stirring 6h under the conditions of 21 DEG C,
Crystal to pyrroloquinoline quinone.
Embodiment 3
A kind of use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, including following step
Rapid:
(1) prepare: utilize pyrroloquinoline quinone to produce the bacterium preparation fermentation liquid containing pyrroloquinoline quinone, method particularly includes: will
Gluconobacter oxvdans, according to the inoculum concentration of 5%, is inoculated in fermentation medium, and under the conditions of 28 DEG C, 3d is cultivated in concussion,
To seed liquor；Then by seed liquor according to the inoculum concentration of 10%, being inoculated in enrichment medium, under the conditions of 28 DEG C, concussion is cultivated
5d, culture fluid 5 DEG C, 9000r/min be centrifuged 15min, collect supernatant, and obtaining pyrroloquinoline quinone concentration is 100-200mg/L's
Fermentation liquid rich in pyrroloquinoline quinone；
(2) extract and separate: use molecular engram solid phase extraction method to extract the fermentation liquid containing pyrroloquinoline quinone, will
Fermentation liquid containing pyrroloquinoline quinone is splined in the molecular engram extraction column being filled with molecularly imprinted polymer, and molecular engram gathers
Solid-to-liquid ratio 1:400 (g/ml) of compound and fermentation liquid containing pyrroloquinoline quinone, the most successively with the ethanol of 10 times of column volumes with
The ethanol of 10 times of column volumes-trifluoroacetic acid mixed liquor eluting, collects ethanol-trifluoroacetic acid mixing eluent, concentrating under reduced pressure, obtains
Pyrroloquinoline quinone crude product；The vacuum of concentrating under reduced pressure is 0.9MPa, and rotating speed is 180 revolutions per seconds；
In described ethanol-trifluoroacetic acid mixed liquor, the volume ratio of ethanol and trifluoroacetic acid is 9:2；
The preparation method of described molecularly imprinted polymer is: by 250mg pyrroloquinoline quinone, 0.4mL methacrylic acid, add
Enter in 150mL acetonitrile solution, room temperature mechanical stirring 15h；Being subsequently adding 500mg silicon dioxide microsphere, ultrasound wave disperses；Add again
Enter 0.9mL ethylene glycol dimethacrylate and 100mg azodiisobutyronitrile, be passed through nitrogen 5min, seal, be heated to 60 DEG C,
Mechanic whirl-nett reaction 15h, separates, obtains polymeric material；By polymeric material with after washing with alcohol 10 times, 50 DEG C are vacuum dried 12h；
With volume ratio 9:1 ethanol/dried polymeric material of acetic acid mixed solution eluting repeatedly, remove the template molecule in polymeric material
Pyrroloquinoline quinone, until can't detect template molecule pyrroloquinoline quinone, finally with gathering after distilled water wash eluting in eluent
Condensation material, to neutral, be dried 12h, obtain molecularly imprinted polymer under 50 DEG C of vacuum, standby；
The particle diameter of described silicon dioxide microsphere is 100-200 mesh；
(3) purification: pyrroloquinoline quinone crude product ultra-pure water is dissolved to the final concentration of 11g/L of pyrroloquinoline quinone, adds salt
Acid for adjusting pH, to 3.2, adds ethanol, and ultra-pure water is 4:1 with the volume ratio of ethanol, stands 16h after stirring 5h under the conditions of 23 DEG C,
Obtain the crystal of pyrroloquinoline quinone.
Embodiment 4
A kind of use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, including following step
Rapid:
(1) prepare: utilize pyrroloquinoline quinone to produce the bacterium preparation fermentation liquid containing pyrroloquinoline quinone, method particularly includes: will
Gluconobacter oxvdans, according to the inoculum concentration of 5%, is inoculated in fermentation medium, and under the conditions of 28 DEG C, 3d is cultivated in concussion,
To seed liquor；Then by seed liquor according to the inoculum concentration of 10%, being inoculated in enrichment medium, under the conditions of 28 DEG C, concussion is cultivated
5d, culture fluid 5 DEG C, 9000r/min be centrifuged 15min, collect supernatant, and obtaining pyrroloquinoline quinone concentration is 100-200mg/L's
Fermentation liquid rich in pyrroloquinoline quinone；
(2) extract and separate: use molecular engram solid phase extraction method to extract the fermentation liquid containing pyrroloquinoline quinone, will
Fermentation liquid containing pyrroloquinoline quinone is splined in the molecular engram extraction column being filled with molecularly imprinted polymer, and molecular engram gathers
Solid-to-liquid ratio 1:500 (g/ml) of compound and fermentation liquid containing pyrroloquinoline quinone, the most successively with the ethanol of 10 times of column volumes with
The ethanol of 10 times of column volumes-formic acid mixed liquor eluting, collects ethanol-formic acid mixing eluent, concentrating under reduced pressure, obtains pyrroles's quinoline
Quinone crude product；The vacuum of concentrating under reduced pressure is 0.9MPa, and rotating speed is 150 revolutions per seconds；
In described ethanol-formic acid mixed liquor, the volume ratio of ethanol and formic acid is 9:1；
The preparation method of described molecularly imprinted polymer is: by 300mg pyrroloquinoline quinone, 0.5mL methacrylic acid, add
Enter in 200mL acetonitrile solution, room temperature mechanical stirring 20h；Being subsequently adding 800mg silicon dioxide microsphere, ultrasound wave disperses；Add again
Enter 1.0mL ethylene glycol dimethacrylate and 150mg azodiisobutyronitrile, be passed through nitrogen 5min, seal, be heated to 70 DEG C,
Mechanic whirl-nett reaction 8h, separates, obtains polymeric material；By polymeric material with after washing with alcohol 10 times, 50 DEG C are vacuum dried 16h；
With volume ratio 10:1 ethanol/dried polymeric material of acetic acid mixed solution eluting repeatedly, the template removed in polymeric material is divided
Sub-pyrroloquinoline quinone, until can't detect template molecule pyrroloquinoline quinone, finally with after distilled water wash eluting in eluent
Polymeric material, to neutral, be dried 12h, obtain molecularly imprinted polymer under 50 DEG C of vacuum, standby；
The particle diameter of described silicon dioxide microsphere is 100-200 mesh；
(3) purification: pyrroloquinoline quinone crude product ultra-pure water is dissolved to the final concentration of 12g/L of pyrroloquinoline quinone, adds salt
Acid for adjusting pH, to 3.6, adds ethanol, and ultra-pure water is 5:1 with the volume ratio of ethanol, stands 12h after stirring 6h under the conditions of 25 DEG C,
Obtain the crystal of pyrroloquinoline quinone.
Experimental example
1, product analysis
1.1, ultraviolet spectral analysis
Using ultraviolet spectrometer to detect the crystallized product of the present invention with PQQ standard substance, result is shown in Fig. 2 simultaneously.Knot
Fruit shows, the crystallized product of the present invention is consistent with the UV adsorption results of PQQ standard substance.
1.2, mass spectral analysis
Using mass spectrograph to detect the crystallized product of the present invention, result is shown in Fig. 3.By the crystallized product mass spectrum of the present invention
Data contrast with known PQQ mass spectrometric data, and result shows, the crystallized product of the present invention is consistent with known PQQ mass spectrometric data.
1.3, HPLC method detection
Detection method: use Waters Symmetry 300C18Liquid-phase chromatographic column, with acetonitrile: (acetonitrile and water all contain water
2% formic acid) it is flowing phase, flow velocity 1mL/min, gradient elution (30-90%, 30min), detects wavelength 230nm.
Detection object: extraction eluent (ethanol-organic acid mixing eluent), PQQ crystallized product.
Interpretation of result: HPLC analysis result see Fig. 4,5.Fig. 4 shows, has the object of high level in extraction eluent,
Extraction has higher selectivity；Fig. 5 shows, by recrystallization, crystallized product purity reaches more than 99%.
1.4, the response rate
With the fermentation liquid of embodiment 1-4 pyrroloquinoline quinone as sample solution, study this molecular engram solid phase extraction to pyrroles
The separating effect of quinoline quinone, uses pyrroloquinoline quinone in high-efficient liquid phase chromatogram technology detection ethanol-organic acid mixing eluent
Content, calculates the response rate (table 1).
The interpretation of result of the table 1 PQQ of the present invention response rate
Table 1 result shows, the effect of pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method of the present invention
The best, the response rate is up to more than 90%.
2, the mensuration of molecularly imprinted polymer adsorption capacity
Weigh in 10mg molecularly imprinted polymer adsorption tube, respectively add 10mL mass concentration be respectively 2,4,8,9,
10,11, the PQQ standard solution of 12g/L, under room temperature, static adsorption 12h, centrifugal, separate absorption saturated after molecular engram polymerization
Solution after thing and absorption, in the solution after absorption, the mass concentration of PQQ uses high-performance liquid chromatogram determination (table 2).
The interpretation of result (mass concentration unit: g/L) of table 2 molecularly imprinted polymer of the present invention adsorption capacity
Table 2 result shows, molecularly imprinted polymer prepared by the present invention has the most special, selection adsorbed target thing pyrrole
Coughing up the performance of quinoline quinone, maximum adsorption capacity is up to 10mg/mg (molecularly imprinted polymer).
Claims (10)
1. use the method for pyrroloquinoline quinone in the isolated and purified fermentation liquid of molecular engram solid phase extraction method, it is characterised in that include
Following steps:
(1) prepare: utilize pyrroloquinoline quinone to produce the bacterium preparation fermentation liquid containing pyrroloquinoline quinone；
(2) extract and separate: use molecular engram solid phase extraction method that the fermentation liquid containing pyrroloquinoline quinone is extracted, will contain
The fermentation liquid of pyrroloquinoline quinone is splined in the molecular engram extraction column being filled with molecularly imprinted polymer, uses ethanol the most successively
With ethanol-organic acid mixed liquor eluting, collect ethanol-organic acid mixing eluent, concentrating under reduced pressure, obtain pyrroloquinoline quinone thick
Product；
(3) purification: pyrroloquinoline quinone crude product ultra-pure water is dissolved, regulates pH to 3-4, add ethanol, stand after stirring, obtain
Pyrroloquinoline quinone.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that described molecularly imprinted polymer is with silicon dioxide as base material, pyrroloquinoline quinone is template molecule, first
Base acrylic acid is function monomer, and ethylene glycol dimethacrylate is cross-linking agent, uses thermal polymerization technology to be prepared from.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 2
Method, it is characterised in that the preparation method of described molecularly imprinted polymer is: by 100-300mg pyrroloquinoline quinone, 0.1-
0.5mL methacrylic acid, joins in 50-200mL acetonitrile solution, room temperature mechanical stirring 6-20h；It is subsequently adding 100-800mg
Silicon dioxide microsphere, ultrasound wave disperses；Add 0.6-1.0mL ethylene glycol dimethacrylate and 50-150mg azo two is different
Butyronitrile, is passed through nitrogen 5min, seals, is heated to 50-70 DEG C, and mechanic whirl-nett reaction 8-20h separates, obtains polymeric material；Will be poly-
Condensation material is with after washing with alcohol 5-10 time, and 50 DEG C are vacuum dried 8-16h；Anti-with volume ratio 6-10:1 ethanol/acetic acid mixed solution
After backwashing takes off dried polymeric material, removes the template molecule pyrroloquinoline quinone in polymeric material, until detecting not in eluent
To template molecule pyrroloquinoline quinone, finally with the polymeric material after distilled water wash eluting to neutral, 50 DEG C of vacuum drying 12h,
Obtain molecularly imprinted polymer, standby；
The particle diameter of described silicon dioxide microsphere is 100-200 mesh.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that described molecularly imprinted polymer and solid-to-liquid ratio 1:100-500 of the fermentation liquid containing pyrroloquinoline quinone
(g/ml)。
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that in described ethanol-organic acid mixed liquor, the volume ratio of ethanol and organic acid is 8-10:1-2；Described
Organic acid is any one in formic acid, acetic acid or trifluoroacetic acid.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that the vacuum of described concentrating under reduced pressure is 0.9MPa, rotating speed is 150-180 revolutions per second.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that in step (3), pyrroloquinoline quinone crude product ultra-pure water is dissolved to final concentration of 9-12g/L.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that in step (3), ultra-pure water is 3-5:1 with the volume ratio of ethanol.
The side of pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that in step (3), regulation pH agents useful for same is hydrochloric acid.
Pyrroloquinoline quinone in the employing isolated and purified fermentation liquid of molecular engram solid phase extraction method the most according to claim 1
Method, it is characterised in that in step (3), whipping temp is 20-25 DEG C, mixing time is 5-6h, and time of repose is 12-24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610515247.6A CN106188042B (en) | 2016-07-01 | 2016-07-01 | The method that PQQ in zymotic fluid is isolated and purified using molecular engram solid phase extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610515247.6A CN106188042B (en) | 2016-07-01 | 2016-07-01 | The method that PQQ in zymotic fluid is isolated and purified using molecular engram solid phase extraction method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106188042A true CN106188042A (en) | 2016-12-07 |
| CN106188042B CN106188042B (en) | 2018-01-05 |
Family
ID=57465633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610515247.6A Active CN106188042B (en) | 2016-07-01 | 2016-07-01 | The method that PQQ in zymotic fluid is isolated and purified using molecular engram solid phase extraction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106188042B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111635402A (en) * | 2020-06-24 | 2020-09-08 | 北大方正集团有限公司 | Separation and purification method of pyrroloquinoline quinone |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112194658A (en) * | 2020-10-23 | 2021-01-08 | 内蒙古拜克生物有限公司 | Separation and purification method of pyrroloquinoline quinone |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104892597A (en) * | 2015-05-14 | 2015-09-09 | 郑州轻工业学院 | Complex extraction method for separation and purification of pyrroloquinoline quinine in fermentation broth |
| CN105294687A (en) * | 2015-12-02 | 2016-02-03 | 郑州轻工业学院 | Ion pair dual water phase method for extracting and separating pyrroloquinoline quinone |
-
2016
- 2016-07-01 CN CN201610515247.6A patent/CN106188042B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104892597A (en) * | 2015-05-14 | 2015-09-09 | 郑州轻工业学院 | Complex extraction method for separation and purification of pyrroloquinoline quinine in fermentation broth |
| CN105294687A (en) * | 2015-12-02 | 2016-02-03 | 郑州轻工业学院 | Ion pair dual water phase method for extracting and separating pyrroloquinoline quinone |
Non-Patent Citations (2)
| Title |
|---|
| 王岩: "酰胺类除草剂分子印迹微球的制备、表征及固相萃取应用", 《中国博士学位论文全文数据库（工程科技I辑）》 * |
| 陈方方等: "分子印迹固相萃取技术在天然产物有效成分分离分析中的应用进展", 《色谱》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111635402A (en) * | 2020-06-24 | 2020-09-08 | 北大方正集团有限公司 | Separation and purification method of pyrroloquinoline quinone |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106188042B (en) | 2018-01-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108026132A (en) | A kind of purification process of nicotinamide mononucleotide | |
| CN104328155B (en) | Utilize the method and application of bacillus of oxidizing glucose production pyrroloquinoline quinone | |
| US20100204526A1 (en) | Process for solvent production utilizing liquid phase adsorption | |
| CN102898566A (en) | Preparation method of metal organic framework molecularly imprinted polymer used for enriching trace amounts of Metolcarb | |
| CN104892597B (en) | PQQ in complexing abstraction separation and purification zymotic fluid | |
| CN102584918A (en) | Method for preparing high-purity baicalin | |
| CN108970589A (en) | A kind of hydrotalcite plural gel ball and its preparation method and application | |
| CN106188042B (en) | The method that PQQ in zymotic fluid is isolated and purified using molecular engram solid phase extraction method | |
| US10316052B2 (en) | Fidaxomicin purification method | |
| CN110437053B (en) | Method for extracting and separating eupatorium adenophorum ketone compounds from eupatorium adenophorum | |
| CN104892493A (en) | Separation and purification method of 2,3-dimethylpyridine | |
| Ma et al. | Preparative purification of salidroside from Rhodiola rosea by two‐step adsorption chromatography on resins | |
| CN102924572A (en) | Method for preparing high-purity daptomycin | |
| CN102311486B (en) | Method for separating and extracting enramycin by using macroporous weakly-acidic cationic resin | |
| CN103232525B (en) | Method for extracting polyoxin from streptomyces fermentation liquor | |
| CN104256640A (en) | Method for extracting natural antioxidant substances from naseberry leaves | |
| Wu et al. | Recovery and purification of paclitaxel using low‐pressure liquid chromatography | |
| CN101066989A (en) | Process of separating and purifying glutathione from fermented liquid in a four-area simulated moving bed | |
| CN105294687B (en) | The method of ion pair Bi-aqueous extraction pyrroloquinoline quinone | |
| CN101429228A (en) | Method for extracting and separating glutathione from glutathione fermentation liquor | |
| CN104497632B (en) | A kind of method utilizing the red wood carthamin that continues of macroporous resin-active carbon compound adsorbent purification | |
| CN100393691C (en) | Method for preparing mandelic acid by macroporous adsorptive resin | |
| CN113511967B (en) | Method for extracting quinic acid from ginkgo leaf extract chromatographic wastewater | |
| CN111135810B (en) | Preparation method of special chromatographic separation medium for cannabidiol separation | |
| CN100526296C (en) | Method for purifying and enriching benzene acryloyl 5- hydroxytryptamine derivant in safflower seed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 450002 No. 5 Dongfeng Road, Jinshui District, Henan, Zhengzhou Patentee after: Zhengzhou University of light industry Address before: 450002 No. 5 Dongfeng Road, Jinshui District, Henan, Zhengzhou Patentee before: ZHENGZHOU University OF LIGHT INDUSTRY |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220916 Address after: Room N06, 3rd Floor, Building 3, Zhengzhou Tianjian Lake Big Data Industrial Park, No. 173, Fengxiang Street, High-tech Industrial Development Zone, Zhengzhou City, Henan Province 450007 Patentee after: Zhengzhou Nietzsche Biotechnology Co.,Ltd. Address before: 450002 No. 5 Dongfeng Road, Jinshui District, Henan, Zhengzhou Patentee before: Zhengzhou University of light industry |