CN106178130A - The formation system of bifurcation structure three-dimensional layering intravascular stent and method - Google Patents

The formation system of bifurcation structure three-dimensional layering intravascular stent and method Download PDF

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CN106178130A
CN106178130A CN201610538244.4A CN201610538244A CN106178130A CN 106178130 A CN106178130 A CN 106178130A CN 201610538244 A CN201610538244 A CN 201610538244A CN 106178130 A CN106178130 A CN 106178130A
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mould
intravascular stent
micro pump
dimensional
hydrogel
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CN106178130B (en
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刘媛媛
蒋维健
谢明亮
胡庆夕
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University of Shanghai for Science and Technology
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/82—Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C67/00—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00
    • B29C67/24—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00 characterised by the choice of material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y70/00—Materials specially adapted for additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F30/00—Computer-aided design [CAD]
    • G06F30/10—Geometric CAD
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
    • A61F2240/001—Designing or manufacturing processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/22—Materials or treatment for tissue regeneration for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus

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Abstract

The invention discloses formation system and the method for a kind of bifurcation structure three-dimensional layering intravascular stent.Native system includes: mold system, hydrogel perfusion system, expendable material print system and computer control system, described mold system is manufactured by 3D printing technique；Described hydrogel perfusion system combines mold system, is driven by micro pump, it is achieved the filling process of hydrogel solution；Described expendable material print system drives micro pump to press specified path by three-dimensional motion mechanism and moves, it is achieved the printing of expendable material；Described computer control system drives three-dimensional motion mechanism motion.The present invention, based on hydrogel perfusion principle and expendable material principle, manufactures mould by 3D printing technique, uses the mode of priming petock gel in mould, carries out successively preparing of intravascular stent；Utilize expendable material, realize the hollow structure of intravascular stent by first adding the method sacrificed afterwards, ultimately form the three-dimensional layering intravascular stent with bifurcation structure.

Description

The formation system of bifurcation structure three-dimensional layering intravascular stent and method
Technical field
The present invention relates to formation system and the method for a kind of bifurcation structure three-dimensional layering intravascular stent, can realize that there is layering Furcated blood vessel stent in the three dimensions of structure, is applied to machine-building and Biotechnology field.
Background technology
In recent years, cardiovascular disease is increasingly becoming the key factor posed a health risk, owing to lacking suitable autologous vein, often There is a large amount of client need synthetic vascular grafts in year.Structure has functional tissue blood vessel support and has broad application prospects.
Vessel diameter in human body is from 5 microns to 25 millimeters, and larger-size blood vessel wall has an obvious three-decker: Inner membrance, middle film and adventitia.Inner membrance is attached to the monolayer endothelial cell on basement membrane；Middle film is by substantial amounts of smooth muscle cell or bullet Property organizational composition；Adventitia is mainly by comprising fibroblast and blood vessel.
Perineural extracellular matrix collagen forms.
At present, in terms of scaffold for vascular tissue engineering forming technology, the process being frequently used can be largely classified into two Class a: class is based on the pre-established method of vascular pattern；Another kind of is the method generated based on blood vessel network in organizational structure.Blood vessel The pre-established method of model can be divided into again the method utilizing die casting and combining Electrospinning shaping；And based on organizational structure The method that interior blood vessel network generates is mainly by 3D cell culture technology, utilizes endotheliocyte etc. spontaneously in biological support Form trickle passage.Although these traditional methods obtain more successful intravascular stent or have the support of blood vessel network, but It is that the process preparing multilamellar intravascular stent at present is difficulty with the structure in scaffold three-dimensional space and the form of vascular bifurcation, And the process being capable of certain three-dimensional branched flow passage structure is difficulty with the three-decker of blood vessel.And for tissue work Journey is for clinical application, and the Furcated blood vessel stent that preparation has in the three dimensions of vessel hierarchies has particularly significant Meaning.Additionally, in the three-decker of blood vessel, because inner membrance is the endotheliocyte of monolayer, therefore when preparing intravascular stent, Only need to construct the two layer entities supporting structure corresponding to middle film and adventitia, inner membrance can be formed by inoculation endotheliocyte.
In the technique preparing scaffold for vascular tissue engineering, the method being frequently used expendable material reaches to form final blood The purpose of pipe holder hollow structure.Conventional expendable material is divided into water-soluble material and heat-fusible materials.Polyvinyl alcohol is conventional Water solublity expendable material, it is completely soluble at a temperature of 65 to 75 DEG C；Pluronic F127 material is conventional hot melt Property expendable material, belongs to thermally reversible material, and water soluble, below gelling temp, can experience by the transformation of gel state to liquid, Therefore can be used as the expendable material in test, the i.e. method by reducing temperature to remove it, thus obtain the pipeline knot of hollow Structure.
3D printing technique (increment manufacturing technology) is a kind of novel Machine Manufacturing Technology of development in recent years, belongs to quick The one of forming technique.Appliance computer Aided Design (CAD) software design go out need processing model, by software hierarchy from Dissipate and numerical control formation system, utilize the mode such as laser beam, hot melt nozzle metal dust, ceramic powders, plastic or other material to be carried out Successively pile up and cohere, final superposition forming.3D printing technique can apply to the printing speed of mould, can realize having complicated sky Between the manufacture of mould of structure, substantially reduce the cycle of Making mold.
Summary of the invention
It is an object of the invention to the defect existed for existing intravascular stent preparation technology, it is provided that a kind of bifurcation structure is three-dimensional The formation system of layering intravascular stent and method, this system manufactures mould by 3D printing technique, by priming petock in mould The mode of gel, carries out successively preparing of intravascular stent；Utilize pluronic F127 expendable material, sacrifice afterwards by first adding Method realizes the hollow structure of intravascular stent, ultimately forms the three-dimensional layering intravascular stent with bifurcation structure.
For reaching above-mentioned purpose, the present invention uses following technical proposals:
A kind of three-dimensional layering intravascular stent formation system with bifurcation structure, including mold system, hydrogel perfusion be System, expendable material print system and computer control system, it is characterised in that: described mold system is by design mould Cad file, uses 3D printer to carry out the printing of each mould；Described hydrogel perfusion system, promotes note by micro pump Emitter piston, is filled into hydrogel and is merged in the runner formed by two moulds；Described expendable material print system, will Expendable material loads in injector syringe, and syringe is fixed on micro pump, and micro pump is arranged on three-dimensional motion mechanism, passes through Three-dimensional motion mechanism drives micro pump motion, micro pump pushing syringe piston extrusion expendable material, thus realizes expendable material Printing.
Described mold system comprises 5 moulds altogether, wherein mould 1 can respectively with mould 2, mould 3, mould 4, mould 5 agree with.Protruding or the recessed path of each mould is identical.
Described hydrogel perfusion system is by micro pump controller, micro pump actuator, syringe piston cylinder, entrance Conduit, delivery channel and above-mentioned mould composition.
Described expendable material print system is by three-dimensional motion mechanism, micro pump controller, micro pump actuator, injection Device piston barrel and syringe needle composition.
Described computer control system includes that a computer system couples a control system, and control system couples control The motor of three-dimensional motion mechanism processed.
The manufacturing process of a kind of three-dimensional layering intravascular stent utilizing said system preparation to have bifurcation structure, beats based on 3D Die has, and carries out test operation by priming petock gel and printing expendable material pluronic F127, it is characterised in that:
1) shape intravascular stent outer layer lower half storey: by 2-in-1 with mould for mould 1 and together with, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 2, available intravascular stent outer layer Lower half Rotating fields；
2) the lower half storey of intravascular stent internal layer is shaped: mould 1 and mould 3 are combined, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 3, available intravascular stent internal layer Lower half Rotating fields；
3) printing of expendable material: the syringe that will be equipped with pluronic F127 material is fixed on micro pump, micro pump Being fixed in three-dimensional movement platform, computer control system controls three-dimensional movement platform and moves according to the path of design, note Emitter realizes the printing of expendable material under the driving of micro pump.As can be seen here, the movement velocity of syringe needle and the extrusion of material are fast Degree must mate, the integrity in guarantee extrusion path.Print procedure is as it is shown in figure 5, set the syringe driving at micro pump Lower piston movement velocity is v0, the extruded velocity of material is v1, syringe point-to-point speed is v, and syringe internal diameter is d0, extrude material Expect a diameter of d1, extrusion flow is Q.Then extrusion flow can represent in order to formula (1) and formula (2):
Q = v 0 d 0 2 4 π - - - ( 1 )
Q = v 1 d 1 2 4 π - - - ( 2 )
By flow principle it follows that
v 0 d 0 2 4 π = v 1 d 1 2 4 π - - - ( 3 )
Again because material extruded velocity is equal to syringe point-to-point speed, it may be assumed that
V=v1 (4)
Piston movement speed and the relation of syringe point-to-point speed can be drawn by formula (3) and (4):
v v 0 = d 0 2 d 1 2 - - - ( 5 )
4) the upper half storey of intravascular stent internal layer is shaped: mould 1 and mould 4 are combined, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 4, available intravascular stent internal layer Upper half Rotating fields；
5) the upper half storey of intravascular stent outer layer is shaped: mould 1 and mould 5 are combined, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 5, available intravascular stent outer layer Upper half Rotating fields；
6) removal of expendable material: reduce system ambient temperature and make the liquefaction of pluronic F127 material flow out, thus formed The pipeline configuration of hollow.Support is taken off from mould 1, i.e. can obtain the three-dimensional layering intravascular stent with bifurcation structure.
The present invention compared with prior art, has and the most obviously highlights substantive distinguishing features and remarkable advantage:
1) present invention utilizes 3D printing technique to carry out the manufacture of mould, can produce designed within the shortest cycle Mould.
2) cad model of corresponding mould can be generated according to existing medical treatment blood vessel data model, and printed by 3D Mode carry out the manufacture of mould such that it is able to build and there is the intravascular stent of real structure in simulation organism.
3) can realize that there is double-deck intravascular stent, it is thus possible to the preferably physiology of the biological body vessel of simulation Structure.
4) intravascular stent with bifurcation structure can be realized, therefore there is shaping single vessel support technique incomparable Advantage.
5) intravascular stent with certain three dimensional structure can be realized, the most more can meet the demand of clinical transplantation.
6) difference of hydrogel material between intravascular stent different layers can be realized, and the variety classes in blood vessel different layers Cell the most different for the requirement kind of surrounding substrate, therefore provide good inoculation condition for follow-up cell inoculation.
In sum, system of the present invention utilizes mold system, hydrogel perfusion system and expendable material print system Comprehensively achieve the shaping of the three-dimensional layering intravascular stent with bifurcation structure.This system has simple and reliable for structure, automatization The advantages such as degree is high, easy to control, the cycle is short, it is adaptable to there is in organizational project the blood of the three-dimensional layering multiple material of bifurcation structure Pipe holder shapes.
Accompanying drawing explanation
Fig. 1 is the formation system of the bifurcation structure three-dimensional layering intravascular stent of the present invention.
Fig. 2 is mold system.
Fig. 3 is the structural representation of hydrogel perfusion system.
Fig. 4 is expendable material print procedure and the schematic diagram of each parameter.
Fig. 5 is by figure (a)～figure (j), print procedure mold system sectional view (wherein illustrates that each mould is in print procedure Cooperation).
In Fig. 1 to Fig. 3:
I mold system, 11 mould 1,12 mould 2,13 moulds 3,
14 mould 4,15 moulds 5,
II hydrogel perfusion system, 21 micro pump controllers, 22 micro pump actuator first,
23 syringe piston cylinders, 24 entry conductors, 25 delivery channels,
III expendable material print system, 31 three-dimensional motion mechanisms, 32 micro pump actuator second,
33 syringe piston cylinders, 34 syringe needle, 35 micro pump controllers,
4 computer systems,
5 frames.
Detailed description of the invention
Below in conjunction with the accompanying drawings and preferred embodiment be further elaborated the concrete structure of the present invention, operation principle and Work process content:
Embodiment one:
Seeing Fig. 1～Fig. 3, the formation system of this bifurcated structure three-dimensional layering intravascular stent, including frame (5), mould system System (I), hydrogel perfusion system (II), expendable material print system (III) and computer control system (IV), it is characterised in that: Described mold system (I) be placed on the base of frame (5)；Described hydrogel perfusion system (II) is movably arranged on frame (5) On so that it is in syringe needle (34) connect a die entrance conduit (24) of mold system (I)；Described sacrifice material Material print system (III) is arranged on frame (5) and above couples the movable displacement of drive hydrogel perfusion system (II)；Described computer Control system (IV) electrically connects, and controls the three-dimensional of a three-dimensional motion mechanism (31) in expendable material print system (III) and moves With the extrusion that a syringe piston cylinder (34) carries out expendable material.
Embodiment two:
The present embodiment is essentially identical with embodiment one, and special feature is as follows:
Described mold system (I) includes mould 1 (11), mould 2 (12), mould 3 (13), mould 4 (14) and mould 5 (15)；The entrance and exit at mould 1 (11) two ends is connected with entry conductor (24) and delivery channel (25) respectively；At perfusion water-setting During glue, mould 2 (12), mould 3 (13), mould 4 (14) and the protruding or recessed path of mould 5 (15) respectively with mould Match in 1 (11) recessed path.
Described hydrogel perfusion system (II) is by micro pump controller (21), micro pump actuator first (22), injection Device piston barrel (23), entry conductor (24) and delivery channel (25) composition；Syringe piston cylinder (23) is arranged on micro pump Actuator first (22) is upper and is fixed by tight fixed bolt, micro pump actuator first (22) driving in micro pump controller (21) Under Dong, pushing syringe piston barrel (23) carries out the extrusion of hydrogel, syringe piston cylinder (23) outlet and entry conductor (24) being connected, entry conductor (24) inserts the entrance of mould 1 (11), and hydrogel solution flows out from delivery channel (25).
Described expendable material print system (III) is by three-dimensional motion mechanism (31), micro pump actuator second (32), note Emitter piston barrel (33), syringe needle (34) and micro pump controller (35) composition, described syringe needle (34) is installed On syringe piston cylinder (33), syringe piston cylinder (33) is arranged on micro pump actuator second (32) and above and passes through tight Determining bolt to fix, micro pump actuator second (32) is arranged on three-dimensional motion mechanism (31), enters with three-dimensional motion mechanism (31) Row motion, micro pump actuator second (32) under the driving of micro pump controller (35), pushing syringe piston barrel (33) Carrying out the extrusion of expendable material, three-dimensional motion mechanism (31) moves under the driving of computer system (4).
Described computer control system (IV) includes that a computer system (4) couples a control system, controls system System couples the motor of three-dimensional motion mechanism (31).
Embodiment three:
The manufacturing process of this bifurcated structure three-dimensional layering intravascular stent, uses said system to operate, and operating procedure is such as Under:
1) shape intravascular stent outer layer lower half storey: by 2-in-1 with mould for mould 1 and together with, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 2, available intravascular stent outer layer Lower half Rotating fields；
2) the lower half storey of intravascular stent internal layer is shaped: mould 1 and mould 3 are combined, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 3, available intravascular stent internal layer Lower half Rotating fields；
3) printing of expendable material: the syringe that will be equipped with pluronic F127 material is fixed on micro pump, micro pump Being fixed in three-dimensional movement platform, computer control system controls three-dimensional movement platform and moves according to the path of design, note Emitter realizes the printing of expendable material under the driving of micro pump.
4) the upper half storey of intravascular stent internal layer is shaped: mould 1 and mould 4 are combined, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 4, available intravascular stent internal layer Upper half Rotating fields；
5) the upper half storey of intravascular stent outer layer is shaped: mould 1 and mould 5 are combined, make respective conduit axis Overlap.After matched moulds, hydrogel solution is passed through the syringe entry conductor from mould 1 side and injects, until solution is from mould 1 Till the delivery channel of opposite side flows out, after treating hydrogel solution gelation, mold removal 5, available intravascular stent outer layer Upper half Rotating fields；
6) removal of expendable material: reduce system ambient temperature and make the liquefaction of pluronic F127 material flow out, thus formed The pipeline configuration of hollow.Support is taken off from mould 1, i.e. can obtain the three-dimensional layering intravascular stent with bifurcation structure.
Embodiment four:
See Fig. 5, utilize said system, the method preparing a three-dimensional layering intravascular stent with bifurcation structure, behaviour Make step as follows:
1) manufacture of mold system: first pass through 3 d modeling software and carry out the design of mold former, generates corresponding 5 Designing a model of mould, by after file that model conversion is STL form, is input to carry out in 3D printer the printing of mould.Institute Mold materials be ABS plastic, 5 moulds finally obtained are as shown in Figure 1.
2) test material: the hydrogel material used by Perfused vessel support outer layer is gelatin (chemical pure CP, molecular weight [9000-70-8], traditional Chinese medicines): gelatin is dissolved in water, is made into the solution that mass fraction is 10%；Used by Perfused vessel support internal layer Hydrogel material be sodium alginate (chemical pure CP, molecular weight [9005-38-3], traditional Chinese medicines): sodium alginate is dissolved in deionization Water, is configured to the solution that mass fraction is 5%；Expendable material uses pluronic F127 (chemical pure, molecular weight [9003-11- 6], traditional Chinese medicines): pluronic F127 is dissolved in deionized water, is configured to the solution that mass fraction is 30%.
3) the lower half storey of intravascular stent outer layer is shaped: the gelatin solution prepared is loaded syringe piston cylinder (23), Gu Surely arrive on micro pump actuator first (22), and syringe piston cylinder (23) outlet is connected, by mould with entry conductor (24) Tool 1 (11) and mould 2 (12) combine, and make respective conduit axis overlap.After matched moulds, set micro pump controller (21) feed flow is 6mL/min, and micro pump controller (21) drives micro pump actuator first (22), and pushing syringe is lived The gelatin solution entry conductor (24) from mould 1 (11) side is injected by plug tube body (23), until solution from mould 1 (11) another Till the delivery channel (25) of side flows out, after treating gelatin solution gelation, mold removal 2 (12), outside available intravascular stent The lower half Rotating fields of layer；
4) the lower half storey of intravascular stent internal layer is shaped: the sodium alginate soln prepared is loaded syringe piston cylinder (23), it is fixed on micro pump actuator first (22), and by syringe piston cylinder (23) outlet with entry conductor (24) even Connect, mould 1 (11) and mould 3 (13) are combined, makes respective conduit axis overlap.After matched moulds, set micro pump The feed flow of controller (21) is 5mL/min, and micro pump controller (21) drives micro pump actuator first (22), promotes note The sodium alginate soln entry conductor (24) from mould 1 (11) side is injected by emitter piston barrel (23), until solution is from mould Till the delivery channel (25) of tool 1 (11) opposite side flows out, after treating sodium alginate soln gelation, mold removal 3 (13), can Obtain the lower half Rotating fields of intravascular stent internal layer；
5) printing of expendable material: the pluronic F127 material prepared is loaded syringe piston cylinder (33), fixing On micro pump actuator second (32), micro pump actuator second (32) is fixed in three-dimensional movement platform (31), computer The G code of printing path is transferred in three-dimensional movement platform (31) by control system (IV), controls three-dimensional movement platform (31) Motor so that it is move according to the printing path of design, sets speed that three-dimensional movement platform (31) translates as 8mm/s, if The feed flow determining micro pump controller (35) is 534 μ L/min, and micro pump controller (35) drives micro pump actuator second (32), pluronic F127 material is extruded by pushing syringe piston barrel (33).
6) the upper half storey of intravascular stent internal layer is shaped: the sodium alginate soln prepared is loaded syringe piston cylinder (23), it is fixed on micro pump actuator first (22), and by syringe piston cylinder (23) outlet with entry conductor (24) even Connect, mould 1 (11) and mould 4 (14) are combined, makes respective conduit axis overlap.After matched moulds, set micro pump The feed flow of controller (21) is 5mL/min, and micro pump controller (21) drives micro pump actuator first (22), promotes note The sodium alginate soln entry conductor (24) from mould 1 (11) side is injected by emitter piston barrel (23), until solution is from mould Till the delivery channel (25) of tool 1 (11) opposite side flows out, after treating sodium alginate soln gelation, mold removal 4 (14), can Obtain the upper half Rotating fields of intravascular stent internal layer；
7) the upper half storey of intravascular stent outer layer is shaped: the gelatin solution prepared is loaded syringe piston cylinder (23), Gu Surely arrive on micro pump actuator first (22), and syringe piston cylinder (23) outlet is connected, by mould with entry conductor (24) Tool 1 (11) and mould 5 (15) combine, and make respective conduit axis overlap.After matched moulds, set micro pump controller (21) feed flow is 6mL/min, and micro pump controller (21) drives micro pump actuator first (22), and pushing syringe is lived The gelatin solution entry conductor (24) from mould 1 (11) side is injected by plug tube body (23), until solution from mould 1 (11) another Till the delivery channel (25) of side flows out, after treating gelatin solution gelation, mold removal 5 (12), outside available intravascular stent The upper half Rotating fields of layer；
8) removal of expendable material: system is placed in 10min in the environment of 10 DEG C, makes pluronic F127 material fluidized flow Go out, thus form the pipeline configuration of hollow.Support is taken off from mould 1 (11), i.e. can get bifurcation structure three-dimensional layering blood vessel Support.

Claims (6)

1. a formation system for bifurcation structure three-dimensional layering intravascular stent, fills including frame (5), mold system (I), hydrogel Injection system (II), expendable material print system (III) and computer control system (IV), it is characterised in that: described mold system (I) it is placed on the base of frame (5)；Described hydrogel perfusion system (II) is movably arranged in frame (5) so that it is in One syringe needle (34) connects a die entrance conduit (24) of mold system (I)；Described expendable material print system (III) it is arranged on frame (5) and above couples the movable displacement of drive hydrogel perfusion system (II)；Described computer control system (IV) electrical connection, controls the three-dimensional of a three-dimensional motion mechanism (31) in expendable material print system (III) and moves and one Syringe piston cylinder (33) carries out the extrusion of expendable material.
The formation system of bifurcation structure three-dimensional the most according to claim 1 layering intravascular stent, it is characterised in that: described Mold system (I) includes mould 1 (11), mould 2 (12), mould 3 (13), mould 4 (14) and mould 5 (15), on each mould Protruding or recessed path is identical；The entrance and exit at mould 1 (11) two ends respectively with entry conductor (24) and outlet guide Pipe (25) is connected；During priming petock gel, mould 2 (12), mould 3 (13), mould 4 (14) and mould 5 (15) are protruding Or recessed path matches with the recessed path of mould 1 (11) respectively.
The formation system of bifurcation structure three-dimensional the most according to claim 2 layering intravascular stent, it is characterised in that: described Hydrogel perfusion system (II) by micro pump controller (21), micro pump actuator first (22), syringe piston cylinder (23), Entry conductor (24) and delivery channel (25) composition；Syringe piston cylinder (23) is arranged on micro pump actuator first (22) And fixed by tight fixed bolt (24), micro pump actuator first (22), under the connection of micro pump controller (21) drives, pushes away Dynamic syringe piston cylinder (23) carries out the extrusion of hydrogel, and syringe piston cylinder (23) outlet is connected with entry conductor (24) Connecing, entry conductor (24) inserts the entrance of mould 1 (11), and hydrogel solution flows out from delivery channel (25).
The formation system of bifurcation structure three-dimensional the most according to claim 3 layering intravascular stent, it is characterised in that: described Expendable material print system (III) is by three-dimensional motion mechanism (31), micro pump actuator second (32), syringe piston cylinder (33), syringe needle (34) and micro pump controller (35) composition, described syringe needle (34) is arranged on syringe piston On cylinder (33), syringe piston cylinder (33) is arranged on micro pump actuator second (32) and above and is fixed by tight fixed bolt, Micro pump actuator second (32) is arranged on three-dimensional motion mechanism (31), moves with three-dimensional motion mechanism (31), trace Pump actuator second (32) is under the driving of micro pump controller (35), and pushing syringe piston barrel (33) carries out expendable material Extrusion, three-dimensional motion mechanism (31) moves under the driving of computer system (4).
The formation system of bifurcation structure three-dimensional the most according to claim 4 layering intravascular stent, it is characterised in that: described Computer control system (IV) includes that a computer system (4) couples a control system, and control system couples three-dimensional motion The motor of mechanism (31).
6. a manufacturing process for bifurcation structure three-dimensional layering intravascular stent, uses bifurcation structure according to claim 1 The formation system of three-dimensional layering intravascular stent operates, it is characterised in that operating procedure is as follows:
1) shape intravascular stent outer layer lower half storey: by 2-in-1 with mould for mould 1 and together with, make respective conduit axis overlap； After matched moulds, hydrogel solution is injected by the syringe entry conductor from mould 1 side, until solution from mould 1 another Till the delivery channel of side flows out, after treating hydrogel solution gelation, mold removal 2, under available intravascular stent outer layer Half Rotating fields；
2) the lower half storey of intravascular stent internal layer is shaped: mould 1 and mould 3 are combined, make respective conduit axis overlap, After matched moulds, hydrogel solution is injected by the syringe entry conductor from mould 1 side, until solution from mould 1 another Till the delivery channel of side flows out, after treating hydrogel solution gelation, mold removal 3, under available intravascular stent internal layer Half Rotating fields；
3) printing of expendable material: the syringe that will be equipped with pluronic F127 material is fixed on micro pump, and micro pump is fixed In three-dimensional movement platform, computer control system controls three-dimensional movement platform and moves according to the path of design, syringe The printing of expendable material is realized under the driving of micro pump；
4) the upper half storey of intravascular stent internal layer is shaped: mould 1 and mould 4 are combined, make respective conduit axis overlap, After matched moulds, hydrogel solution is injected by the syringe entry conductor from mould 1 side, until solution from mould 1 another Till the delivery channel of side flows out, after treating hydrogel solution gelation, mold removal 4, can get the upper of intravascular stent internal layer Half Rotating fields；
5) the upper half storey of intravascular stent outer layer is shaped: mould 1 and mould 5 are combined, make respective conduit axis overlap, After matched moulds, hydrogel solution is injected by the syringe entry conductor from mould 1 side, until solution from mould 1 another Till the delivery channel of side flows out, after treating hydrogel solution gelation, mold removal 5, can get the upper of intravascular stent outer layer Half Rotating fields；
6) removal of expendable material: reduce system ambient temperature and make the liquefaction of pluronic F127 material flow out, thus form hollow Pipeline configuration, support is taken off from mould 1, i.e. can obtain having the three-dimensional layering intravascular stent of bifurcation structure.
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