CN106174501A - A kind of manufacture method of spirit Chinese holly ferment - Google Patents

A kind of manufacture method of spirit Chinese holly ferment Download PDF

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Publication number
CN106174501A
CN106174501A CN201610562466.XA CN201610562466A CN106174501A CN 106174501 A CN106174501 A CN 106174501A CN 201610562466 A CN201610562466 A CN 201610562466A CN 106174501 A CN106174501 A CN 106174501A
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CN
China
Prior art keywords
ferment
mel
chinese holly
manufacture method
strain
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Pending
Application number
CN201610562466.XA
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Chinese (zh)
Inventor
廖祥儒
江威
梁金溪
赵思洁
蔡宇杰
管政兵
郑晓东
时瑾
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Jiangnan University
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Jiangnan University
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Publication date
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Priority to CN201610562466.XA priority Critical patent/CN106174501A/en
Publication of CN106174501A publication Critical patent/CN106174501A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus

Abstract

The invention provides the manufacture method of a kind of spirit Chinese holly ferment, its process characteristic is with Ganoderma, Fructus Lycii, Fructus Jujubae and Rhizoma Zingiberis Recens as primary raw material, is aided with Mel and strain.Raw material adds Mel and strain (commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus) each 100mL after suitably smashing, fermentation temperature is 30 DEG C, keeps pH=5~8, fermentation time 5~10d.The seasonings such as Mel, sucrose, oligosaccharide, high fructose syrup are added after filtration.Respectively drink 50 100mL, the seasonings such as Mel, oligofructose, oligomeric isomaltose, white sugar, lactobionic acid can be added.Spleen benefiting and stimulating the appetite, benefiting vital QI and blood, tranquilizing by nourishing the heart, replenishing vital essence to improve eyesight, control lassitude, heart beating is had a sleepless night, dizziness and blurred vision.

Description

A kind of manufacture method of spirit Chinese holly ferment
Technical field
A kind of method of fermentable Chinese herbal medicine enzyme beverage, relates to market milk bacillus, bacillus acidophilus and Mus Lee's sugar lactobacillus lyophilized powder, fermentation glossy ganoderma, Fructus Lycii, Fructus Jujubae and process with Rhizoma Zingberis Recens, for the method for ferment, belong to technical field of bioengineering.
Background technology
Ferment one word is the Japanese's a kind of call to enzyme (enzyme), has the polymer substance of biocatalytic Activity, Later the fermentation liquid with bioactive substance obtained through fermentable or fermented product were referred to as ferment.In view of some Producer only focuses on fruit, particularly with the addition of different types of fruit, does not note the effect of microorganism in sweat, also ignores Application to some functional materials, it is necessary to application tradition and science collocation make ferment product, expand and ensure ferment Function, provides safeguard for common people's health.Ferment product development and application are also vulnerable to the impact of living contaminants, and the most also having must Want the fermentation technique of applied science to ensure product quality.
Ganoderma has a following several functions: 1) blood pressure lowering.2) blood sugar lowering.3) blood fat reducing.4) the benefit motive.5) tonifying liver gas.
Fructus Lycii has a following several functions: 1) nourishing the liver to improve visual acuity.2) blood-enriching tranquillizing.3) promoting the production of body fluid to quench thirst.4) nourishing the lung to arrest cough.
Fructus Jujubae has a following several functions: 1) anti-cancer and cancer-preventing.2) blood pressure lowering, cholesterol reducing.3) hepatoprotective.4) prevention bone Matter is loosened.5) prevention cerebral blood supply insufficiency.6) anti-angiocardiopathy.7) regain one's strength.8) prevention cholelithiasis.
Rhizoma Zingiberis Recens has a following several functions: 1) removing cold and wind.2) strengthening the spleen and stomach.3) gastroenteritis is prevented and treated.
Summary of the invention
Inventive formulation and usage: select Ganoderma 120g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g, clean with tap water Surface contaminants.Soaking 3min through 0.9% saline solution, drinking water rinses one time.Strain is with commercially available lactobacillus casei, bacillus acidophilus And lactobacillus rhamnosus, seed is done in the mixing of independent MRS culture medium culturing 18h geometric ratio.Individually MRS culture medium (MRS culture medium prescription For (g/L): beef protein powder 10, oppress juice 10, Yeast diffusion juice powder 5, glucose 20, sodium acetate 5, dibasic ammonium citrate 2, tween 80 0.1, magnesium sulfate 0.58, manganese sulfate 0.28, distilled water 1000mL, regulate pH 6.2~6.4,121 DEG C of autoclavings 15min).Adding Mel and each 100mL of strain, drinking water 10L after suitably being smashed by raw material, fermentation temperature is 30 DEG C, keeps pH=5 ~8, fermentation time 5~10d.By deployed composite enzyme beverage through organic membrane filter machine, filter out small-sized Chinese herbal medicine residue, Obtain spirit Chinese holly ferment.The seasonings such as Mel, sucrose, oligosaccharide, high fructose syrup can be added.Respectively drink 50-100mL, can add The seasonings such as Mel, oligofructose, oligomeric isomaltose, white sugar, lactobionic acid.Spleen benefiting and stimulating the appetite, benefiting vital QI and blood, tranquilizing by nourishing the heart, benefit Shrewd head, controls lassitude, and heart beating is had a sleepless night, dizziness and blurred vision.
Technical scheme:
The purpose of the present invention is to propose to a kind of Chinese medicine formula method for preparing beverage, it is without expensive device, can be prevented effectively from Fungus and the generation of pathogenic bacterium toxin, and can stablize persistently there is good health-care effect.
Commercially available lactobacillus casei, lactobacillus rhamnosus, bacillus acidophilus, independent MRS culture medium culturing 18h is mixed by geometric ratio For strain, utilize Ganoderma, Fructus Lycii, Fructus Jujubae and Rhizoma Zingiberis Recens fermentation preparation enzyme beverage.Being divided into three phases, first stage is that raw material is clear Washing, second stage is anaerobic fermentation, and the phase III is seasoning.
Implementing process is:
1) the selecting and processing of material: select Ganoderma 120g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g, with washing from the beginning Clean surface contaminants.Soaking 3min through 0.9% saline solution, drinking water rinses one time.
2) by commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus, independent MRS culture medium culturing 18h geometric ratio Seed is done in mixing.
3) adding Mel and each 100mL of strain, drinking water 10L after suitably smashing, ferment 5~10d, pH=6.0~8.0, temperature Spend 30 DEG C.
4) later stage processes: by deployed composite enzyme beverage through organic membrane filter machine, filter out small-sized Chinese herbal medicine residue, Obtain spirit Chinese holly ferment.The seasonings such as Mel, sucrose, oligosaccharide, high fructose syrup can be added.
Beneficial effects of the present invention:
(1) by strictly controlling fermentation temperature, effectively reduce the loss of spirit Chinese holly ferment nutrient, remain to greatest extent Enzyme activity.
(2) having accessed lactobacillus, bacillus acidophilus and lactobacillus rhamnosus, dominant strain inhibits other miscellaneous bacterias to grow, Make the steady quality of product.
(3) total time fermented is 5~10d, and the time is short to be avoided polyoxygenated, was conducive to keeping Ganoderma etc. itself to contain Ferment material, and the flavor substance itself contained.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is described further.Following example are only the several concrete reality of the present invention Execute example, but the design concept of the present invention is not limited thereto, all changes utilizing this design that the present invention carries out unsubstantiality, all The behavior invading scope should be belonged to.
Embodiment 1
Select Ganoderma 120g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g, clean surface contaminants with tap water.Through 0.9% food Saline soak 3min, drinking water rinses one time.By commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus, independent MRS Seed is done in the mixing of culture medium culturing 18h geometric ratio.Mel 100mL and each 100mL of strain, drinking water 10L is added after suitably smashing.Fermentation 5d, pH value 8.0, temperature 30 DEG C.By deployed ferment through organic membrane filter machine, filter out small-sized raw material residue, add after filtration The each 10g/L seasoning of Mel, high fructose syrup, obtains spirit Chinese holly ferment.
Embodiment 2
Select Ganoderma 150g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g, clean surface contaminants with tap water.Through 0.9% food Saline soak 3min, drinking water rinses one time.By commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus, independent MRS Seed is done in the mixing of culture medium culturing 18h geometric ratio.Mel 100mL and each 100mL of strain, drinking water 10L is added after suitably smashing.Fermentation 7d, pH value 8.0, temperature 30 DEG C.By deployed ferment through organic membrane filter machine, filter out small-sized raw material residue, add after filtration The each 10g/L seasoning of Mel, high fructose syrup, obtains spirit Chinese holly ferment.
Embodiment 3
Select Ganoderma 200g, Fructus Lycii 800g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g, clean surface contaminants with tap water.Through 0.9% food Saline soak 3min, drinking water rinses one time.By commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus, independent MRS Seed is done in the mixing of culture medium culturing 18h geometric ratio.Mel 100mL and each 100mL of strain, drinking water 10L is added after suitably smashing.Fermentation 10d, pH value 8.0, temperature 30 DEG C.By deployed ferment through organic membrane filter machine, filter out small-sized raw material residue, add after filtration The each 10g/L seasoning of Mel, high fructose syrup, obtains spirit Chinese holly ferment.
Ganodermas in the present invention etc. are containing abundant natural ferment material and reduce the several functions such as blood glucose, but not only It is only limitted to this, the amount of Ganoderma, Fructus Lycii, Fructus Jujubae and Rhizoma Zingiberis Recens according to practical situation, can be determined or select other suitable medium-height grass Medicine mixes, as long as it is permissible to reach ferment reasonable mixture ratio, ferment content higher position.
Additionally fermenation raw liquid selection and proportioning, connect bacterium amount and be also not necessarily limited to the combination that embodiment is lifted, can be according to actual feelings Condition selects different combinations.
The foregoing is only the preferred embodiments of the present invention, not in order to limit the present invention, all thinkings in the present invention Within principle, any modification, equivalent substitution and improvement etc. done, should be included within the scope of the present invention.

Claims (9)

1. a manufacture method for clever Chinese holly ferment, is characterized in, with Ganoderma, Fructus Lycii, Fructus Jujubae and Rhizoma Zingiberis Recens for dominant fermentation raw material, adding Enter Mel, use commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus, produce preparation spirit Chinese holly enzyme beverage, its work Skill is:
1) select Ganoderma 120g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g, clean surface contaminants with tap water.Through 0.9% Sal Water soaking 3min, drinking water rinses one time.
2) mixing of independent to commercially available lactobacillus casei, bacillus acidophilus and lactobacillus rhamnosus MRS culture medium culturing 18h geometric ratio is done Seed.
3) adding Mel and each 100mL of strain, drinking water 10L after suitably smashing, fermentation temperature is 30 DEG C, keeps pH=5~8, sends out The ferment time 5~10d.
4) by deployed composite enzyme beverage through organic membrane filter machine, filter out small-sized Chinese herbal medicine residue, obtain spirit Chinese holly ferment. Add the seasonings such as Mel, sucrose, oligosaccharide, high fructose syrup.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: fermentation strain uses commercially available cheese Seed is done in the mixing of lactobacillus, bacillus acidophilus and lactobacillus rhamnosus independent MRS culture medium culturing 18h geometric ratio.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: strain MRS culture medium prescription For (g/L): beef protein powder 10, oppress juice 10, Yeast diffusion juice powder 5, glucose 20, sodium acetate 5, dibasic ammonium citrate 2, tween 80 0.1, magnesium sulfate 0.58, manganese sulfate 0.28,1000mL121 DEG C of autoclaving 15min of distilled water, regulate pH=6.2~6.4.
The most according to claim 1 a kind of spirit Chinese holly ferment manufacture method, it is characterized in that: proportioning raw materials be Ganoderma 120g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: material processing is for by spirit Sesame, Fructus Lycii, Fructus Jujubae, gingerade wash clean, soak 3min through 0.9% saline solution, and drinking water rinses one time.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: raw material and Mel, the ratio of strain Example is to add Mel and strain 100mL after every part of raw material (Ganoderma 120g, Fructus Lycii 600g, Fructus Jujubae 200g, Rhizoma Zingiberis Recens 20g) is suitably smashed, Drinking water addition is 10L.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: raw material and strain, Mel mixing Uniformly, fermentation temperature is 30 DEG C, keeps pH=5~8, fermentation time 5~10d.Filter standby.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: filter after fermentation liquid and can add honeybee The seasonings such as honey, sucrose, oligosaccharide, high fructose syrup.
The manufacture method of a kind of spirit Chinese holly ferment the most according to claim 1, is characterized in that: will not be by miscellaneous bacteria in sweat Polluting, after fermentation, ferment quality is good, good mouthfeel.
CN201610562466.XA 2016-07-15 2016-07-15 A kind of manufacture method of spirit Chinese holly ferment Pending CN106174501A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107080130A (en) * 2017-04-19 2017-08-22 句容金坤农业科技有限公司 A kind of preparation method of lucidum spore powder ferment health drink
CN110338322A (en) * 2019-08-02 2019-10-18 辽宁晟启昊天生物医药科技有限公司 A kind of orange soup fermented beverage and preparation method thereof
CN111248384A (en) * 2020-03-27 2020-06-09 陕西巨子生物技术有限公司 Composite probiotic enzyme beverage and preparation method thereof
CN111903885A (en) * 2020-08-24 2020-11-10 南京康普生物技术有限公司 Chinese wolfberry enzyme beverage with bowel relaxing function and preparation method thereof

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CN102669658A (en) * 2012-04-23 2012-09-19 浙江科技学院 Preparation method of plant enzyme food
CN103844266A (en) * 2014-03-25 2014-06-11 容瑜 Natural conditioning and nourishing ferment and manufacturing method thereof
CN104824548A (en) * 2015-04-09 2015-08-12 周学义 Matrimony vine composite health care enzyme and preparation method thereof
CN105495244A (en) * 2016-01-08 2016-04-20 江西果果生物科技有限公司 Method for making shaddock, apple and tomato ferment health-care beverage

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107080130A (en) * 2017-04-19 2017-08-22 句容金坤农业科技有限公司 A kind of preparation method of lucidum spore powder ferment health drink
CN110338322A (en) * 2019-08-02 2019-10-18 辽宁晟启昊天生物医药科技有限公司 A kind of orange soup fermented beverage and preparation method thereof
CN111248384A (en) * 2020-03-27 2020-06-09 陕西巨子生物技术有限公司 Composite probiotic enzyme beverage and preparation method thereof
CN111903885A (en) * 2020-08-24 2020-11-10 南京康普生物技术有限公司 Chinese wolfberry enzyme beverage with bowel relaxing function and preparation method thereof

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