CN106174165A - A kind of short flash extracting method of lycopene - Google Patents
A kind of short flash extracting method of lycopene Download PDFInfo
- Publication number
- CN106174165A CN106174165A CN201610476235.7A CN201610476235A CN106174165A CN 106174165 A CN106174165 A CN 106174165A CN 201610476235 A CN201610476235 A CN 201610476235A CN 106174165 A CN106174165 A CN 106174165A
- Authority
- CN
- China
- Prior art keywords
- lycopene
- filtrate
- filtering residue
- extraction
- volume
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses the short flash extracting method of a kind of lycopene, the steps include: first fresh tomato to be passed through dehydrated alcohol pretreatment, homogenate extraction process is carried out as solvent again by ethyl acetate, and by contrast lycopene standard curve measuring and calculating average recoveries.Present invention extraction time is short, extraction efficiency is high, and it is high by more than 80% that its extraction ratio extracts the extraction ratio of 2 each about 30s extraction ratio than conventional solvent restricted-access media 6h.
Description
Technical field
The present invention relates to the extracting method of a kind of lycopene, particularly relate to the short flash extraction side of a kind of lycopene
Method.
Background technology
More than the 80% of human body lycopene intake is from Fructus Lycopersici esculenti, Fructus Lycopersici esculenti, calls Fructus Lycopersici esculenti, tomato, belongs to eggplant
Section.Originating in the countries such as America Peru, Ecuador, Mexico, China extensively cultivates in north and south.Containing abundant dimension life among Fructus Lycopersici esculenti
The multiple element such as element, mineral, saccharide and calcium, potassium, ferrum, zinc.Fructus Lycopersici esculenti can work as vegetable, can make again fruit and eat.Tomato red
Element is a kind of oxygen-free carotenoid, is a kind of functional natural pigments, has the strongest non-oxidizability, removes freely
The ability of base, also has the function of defying age simultaneously, can improve immunity, prevent the effects such as cardiovascular and cerebrovascular disease, moreover,
Lycopene also has good effect for the middle-aged and elderly people of preventing and treating tumor patient, carcinoma of prostate, uterus carcinoma and immunocompromised,
It it is the focus in current food research field.The functional food additives that lycopene is universally acknowledged, has nutrition coloring dual
Effect.
Lycopene belongs to fat-soluble pigment, water insoluble, is insoluble in intensive polar solvent methanol, ethanol etc., dissolve in benzene,
The organic solvents such as chloroform, ether, petroleum ether, ethane, are dissolved in aliphatic hydrocarbon, aromatic hydrocarbon and chlorohydrocarbon, and lycopene is in plant
Natural red pigments, has the last one absworption peak at 485nm wavelength.Conventional solvent extraction uses fresh tomato to extract lycopene
Time, containing substantial amounts of moisture in fresh tomato, solvent is not easy to penetrate into and intracellular causes extraction efficiency relatively low, side most commonly
Formula be Fructus Lycopersici esculenti is dried, be ground into powder after extract again, owing to lycopene is thermal sensitivity and photosensitive material, dry process
The loss of content of lycopene can be caused.
Summary of the invention
It is an object of the invention to provide the short flash extracting method of a kind of lycopene, pre-by setting up ethanol dehydration
Process and extract, with organic solvent mishap, the technique combined, control Fructus Lycopersici esculenti pretreatment mode, Extraction solvent, extraction time, extraction
Machine gear, solid-liquid ratio are to improve the extraction ratio of lycopene.
For achieving the above object, the present invention is by the following technical solutions:
The short flash extracting method of a kind of lycopene in the present invention, specifically includes following concrete technology step:
(1) taking sample fresh tomato to clean stripping and slicing and put into Ice-making machine, 1:2 adds the ethanol containing 0.05%BHT by volume
Solution, stirs after 20min, 4800r/min are centrifuged 10min and filters, and filtering residue adds ethanol solution again, is again stirring for 20min,
4800r/min filters to get filtrate and filtering residue after being centrifuged 10min；
(2) Fructus Lycopersici esculenti filtering residue step (1) obtained and solvent are during 1:35-45 puts into flash extracter by volume, molten
Agent is ethyl acetate or chloroform, extracts 25-35s, be centrifuged and obtain filtrate and filter under regulation operation range to middling speed (3 grades)
Slag；
(3) volume of the filtrate after determination step (2) is centrifugal and absorbance thereof, calculate the content of lycopene in filtrate
And extraction ratio；
(4) take the Fructus Lycopersici esculenti filtering residue after being centrifuged in step (2) and repeat the extraction process of step (2), after being centrifuged, obtain second time
The Fructus Lycopersici esculenti filtrate extracted and filtering residue；
(5) volume of the filtrate that second time is extracted and absorbance thereof in determination step (4), calculate lycopene in filtrate
Content and extraction ratio.
Further, in described step (1), ethanol solution is ethanol solution.
Further, in described step (2), Fructus Lycopersici esculenti filtering residue is 1:40 with the volume ratio of solvent, and described solvent is acetic acid
Ethyl ester.
Further, filtrate content of lycopene and the measuring method of extraction ratio in described step (3) and step (5)
For:
A. lycopene standard curve is made with S Ⅰ pigment: weigh 0.0250g S Ⅰ pigment, use dehydrated alcohol
Move into 50mL volumetric flask, and be settled to scale, shake up to obtain lycopene storing solution；Accurately draw this storing solution 10mL to hold in 50mL
Measuring bottle, dehydrated alcohol is settled to scale, shakes up；Draw 0.5 respectively, 1.0,1.5,2.0,2.5mL in 50mL volumetric flask, with anhydrous
Ethanol dilution to scale, shake up concentration is respectively the lycopene standard solution of 0.5,1.0,1.5,2.0,2.5 μ g/mL.With
Dehydrated alcohol is blank sample, and spectral scan determines maximum absorption wavelength, and under the conditions of this maximum absorption wavelength, bioassay standard is molten
Liquid absorbance, with concentration as abscissa, absorbance be vertical coordinate draw lycopene standard curve；
B., after the filtrate dilution after being centrifuged in step (2) and (4), measured according to filtrate lycopene after dilution
Absorbance comparison lycopene standard curve draw the concentration of lycopene；
C. according to equation below calculating lycopene:
Wherein, C suitably dilute according to filtrate after the absorbance concentration of lycopene that converts out, μ g/mL；
The filtrate volume that V sucking filtration obtains, mL；
The quality of m sample fresh tomato, g.
The beneficial effects of the present invention is, step of the present invention (1) uses dehydrated alcohol process 2 times and be conducive to moisture and alcohol
The removing of solubility impurity without causing a large amount of losses of lycopene, and then be conducive to improving the extraction efficiency of lycopene and
Purity；Lycopene extraction ratio in ethyl acetate and chloroform is apparently higher than other organic solvents, and uses acetic acid second
Ester toxicity is less, cost is relatively low；Solid-liquid ratio, extraction time and extraction gear are reasonable, overheated will not cause the loss of lycopene.
Therefore, present invention extraction time is short, extraction efficiency is high, and its extraction ratio extracts the extraction ratio of 2 each about 30s and compares conventional solvent
The extraction ratio of restricted-access media 6h is high by more than 80%.
Accompanying drawing explanation
Fig. 1 is the lycopene standard curve in the short flash extracting method of a kind of lycopene of the present invention
Figure.
Detailed description of the invention
Further illustrate technical scheme below in conjunction with the accompanying drawings and by detailed description of the invention.
The short flash extracting method of a kind of lycopene in the present invention, specifically includes following concrete technology step:
(1) taking dozens of fresh tomato to clean stripping and slicing and put into Ice-making machine, 1:2 adds the nothing containing 0.05%BHT by volume
Hydrous ethanol solution, stirring 20min, 4800r/min filter after being centrifuged 10min, obtain filtering residue and add ethanol solution again, again
Stirring 20min, 4800r/min filter to get filtrate and filtering residue after being centrifuged 10min；
(2) in taking Fructus Lycopersici esculenti filtering residue 5 grams that step (1) obtains and solvent 1:40 putting into flash extracter by volume, molten
Agent is ethyl acetate, and regulation operation range extracts 30s under 3 grades, is centrifuged and obtains Fructus Lycopersici esculenti filtrate and filtering residue；
(3) volume of the filtrate after determination step (2) is centrifugal and absorbance thereof, calculate the content of lycopene in filtrate
And extraction ratio:
(4) take in step (2) centrifugal after Fructus Lycopersici esculenti filtering residue repeat the extraction process of step (2), obtain after secondary centrifuging kind
Eggplant filtrate and filtering residue；
(5) volume of filtrate after secondary centrifuging and absorbance thereof in determination step (4), calculate lycopene in filtrate
Content and extraction ratio.
In this example, in step (3) and (5), extraction ratio measuring method concretely comprises the following steps:
A. lycopene standard curve is made with S Ⅰ pigment: weigh 0.0250g S Ⅰ pigment, use dehydrated alcohol
Move into 50mL volumetric flask, and be settled to scale, shake up to obtain lycopene storing solution；Accurately draw this storing solution 10mL to hold in 50mL
Measuring bottle, dehydrated alcohol is settled to scale, shakes up；Draw 0.5 respectively, 1.0,1.5,2.0,2.5mL in 50mL volumetric flask, with anhydrous
Ethanol dilution to scale, shake up concentration is respectively the lycopene standard solution of 0.5,1.0,1.5,2.0,2.5 μ g/mL.With
Dehydrated alcohol is blank sample, and spectral scan determines maximum absorption wavelength, and under the conditions of this maximum absorption wavelength, bioassay standard is molten
Liquid absorbance, with concentration as abscissa, absorbance be vertical coordinate draw lycopene standard curve；
B., after the filtrate dilution after being centrifuged in step (2) and (4), measured according to filtrate lycopene after dilution
Absorbance comparison lycopene standard curve draw the concentration of lycopene；
C. according to equation below calculating lycopene:
Wherein, C suitably dilute according to filtrate after the absorbance concentration of lycopene that converts out, μ g/mL；
The filtrate volume that V sucking filtration obtains, mL；
The quality of m sample fresh tomato, g.
Based on above-mentioned, the average recoveries recording lycopene is 88.037 μ g/g, and conventional solvent restricted-access media rate
Generally 47 μ g/g, it is seen then that in the present invention, short flash extraction method has feature rapidly and efficiently concurrently.
Skilled person will appreciate that of the industry, the present invention is not restricted to the described embodiments, above-described embodiment and explanation
The principle that the present invention is simply described described in book, without departing from the spirit and scope of the present invention, the present invention also has
Various changes and modifications, these changes and improvements both fall within scope of the claimed invention.Claimed scope
Defined by appending claims and equivalent thereof.
Claims (4)
1. the short flash extracting method of a lycopene, it is characterised in that include following concrete technology step:
(1) taking sample fresh tomato to clean stripping and slicing and put into Ice-making machine, 1:2 adds the ethanol solution containing 0.05%BHT by volume,
Stirring 20min, 4800r/min filter after being centrifuged 10min, obtain filtering residue and add ethanol solution again, are again stirring for 20min,
4800r/min filters to get filtrate and filtering residue after being centrifuged 10min；
(2) Fructus Lycopersici esculenti filtering residue step (1) obtained and solvent are during 1:35-45 puts into flash extracter by volume, and solvent is
Ethyl acetate or chloroform, extract 25-35s under regulation operation range to middling speed, be centrifuged and obtain filtrate and filtering residue；
(3) determination step (2) is centrifuged volume and the absorbance thereof of rear filtrate, calculates content and the extraction of lycopene in filtrate
Rate；
(4) take the Fructus Lycopersici esculenti filtering residue after being centrifuged in step (2) and repeat the extraction process of step (2), after secondary centrifuging, obtain Fructus Lycopersici esculenti filter
Liquid and filtering residue；
(5) volume of filtrate after secondary centrifuging and absorbance thereof in determination step (4), calculates containing of lycopene in filtrate
Amount and extraction ratio.
The short flash extracting method of a kind of lycopene the most according to claim 1, it is characterised in that described step
(1) in, ethanol solution is ethanol solution.
The short flash extracting method of a kind of lycopene the most according to claim 1, it is characterised in that described step
(2) in, Fructus Lycopersici esculenti filtering residue is 1:40 with the volume ratio of solvent, and described solvent is ethyl acetate.
The short flash extracting method of a kind of lycopene the most according to claim 1, it is characterised in that described step
(3) measuring method of filtrate content of lycopene and extraction ratio is and in (5):
A. lycopene standard curve is made with S Ⅰ pigment: weigh 0.0250g S Ⅰ pigment, move into dehydrated alcohol
50mL volumetric flask, and it is settled to scale, shake up to obtain lycopene storing solution；Accurately draw this storing solution 10mL in 50mL capacity
Bottle, dehydrated alcohol is settled to scale, shakes up；Draw 0.5 respectively, 1.0,1.5,2.0,2.5mL is in 50mL volumetric flask, dehydrated alcohol
Be diluted to scale, shake up concentration is respectively the lycopene standard solution of 0.5,1.0,1.5,2.0,2.5 μ g/mL.With anhydrous
Ethanol is blank sample, and spectral scan determines maximum absorption wavelength, and under the conditions of this maximum absorption wavelength, bioassay standard solution is inhaled
Luminosity, with concentration as abscissa, absorbance be vertical coordinate draw lycopene standard curve；
B. after the filtrate dilution after being centrifuged in step (2) and (4), the suction measured according to filtrate lycopene after dilution
Shading value comparison lycopene standard curve draws the concentration of lycopene；
C. according to equation below calculating lycopene:
Wherein, C suitably dilute according to filtrate after the absorbance concentration of lycopene that converts out, μ g/mL；
The filtrate volume that V sucking filtration obtains, mL；
The quality of m sample fresh tomato, g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610476235.7A CN106174165A (en) | 2016-06-27 | 2016-06-27 | A kind of short flash extracting method of lycopene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610476235.7A CN106174165A (en) | 2016-06-27 | 2016-06-27 | A kind of short flash extracting method of lycopene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106174165A true CN106174165A (en) | 2016-12-07 |
Family
ID=57461783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610476235.7A Pending CN106174165A (en) | 2016-06-27 | 2016-06-27 | A kind of short flash extracting method of lycopene |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106174165A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109612957A (en) * | 2019-02-22 | 2019-04-12 | 济宁医学院 | A kind of method of quick detection lycopene |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176577A (en) * | 1994-10-31 | 1998-03-18 | 马克特辛姆化学工厂有限公司 | Stable lycopene concentrates and process for their preparation |
| CN1316468A (en) * | 2001-05-15 | 2001-10-10 | 北京天纯维通生物技术有限公司 | Process for separating and preparing lycopene |
| CN1328093A (en) * | 2001-07-27 | 2001-12-26 | 陶法 | Process for preparing lycopene |
| CN1405235A (en) * | 2002-10-22 | 2003-03-26 | 赵京城 | Method for preparing high-purity lycopene |
-
2016
- 2016-06-27 CN CN201610476235.7A patent/CN106174165A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176577A (en) * | 1994-10-31 | 1998-03-18 | 马克特辛姆化学工厂有限公司 | Stable lycopene concentrates and process for their preparation |
| CN1316468A (en) * | 2001-05-15 | 2001-10-10 | 北京天纯维通生物技术有限公司 | Process for separating and preparing lycopene |
| CN1328093A (en) * | 2001-07-27 | 2001-12-26 | 陶法 | Process for preparing lycopene |
| CN1405235A (en) * | 2002-10-22 | 2003-03-26 | 赵京城 | Method for preparing high-purity lycopene |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109612957A (en) * | 2019-02-22 | 2019-04-12 | 济宁医学院 | A kind of method of quick detection lycopene |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100537586C (en) | Method for producing high purity jasminoidin and high color number gardenia yellow pigment | |
| CN101961427B (en) | Method for extracting polysaccharide and polyphenol of oil-tea camellia husk | |
| CN101891580B (en) | Method for supercritical carbon dioxide extraction of lycopene in watermelon | |
| CN102229633B (en) | Method for separating and preparing five high-purity anthocyanidin monomers from grape skins | |
| CN111789911B (en) | A method for preparing aloe peel extract with eutectic solvent | |
| CN109833377A (en) | A kind of extract from fruit shell of camellia oleifera abel and its preparation method and application | |
| CN105548429B (en) | A kind of method for determining organo-arsenic and inorganic arsenic in aquatic feeds simultaneously | |
| CN108484465B (en) | A kind of method that recyclable aqueous two-phase extracts astaxanthin from haematococcus pluvialis | |
| CN103804507A (en) | Maryland tobacco polysaccharide, extracting and purifying method and application thereof as antioxidant | |
| CN102807770A (en) | Method for preparing paprika red pigment by ultrasonic pre-treatment | |
| CN106174165A (en) | A kind of short flash extracting method of lycopene | |
| CN101514219B (en) | Method for extracting black rice anthocyanins | |
| CN104725387A (en) | Preparation method for preparing sodium copper chlorophyllin from gingko leaves | |
| CN104628798B (en) | The method for preparing anthocyanin and polysaccharide simultaneously from purple dendrobium raw material | |
| CN103725033B (en) | Method for extracting Enteromorpha pigment | |
| CN101831198A (en) | Preparation method of capsanthin | |
| CN103080238B (en) | For the manufacture of the method for the high extracting and enriching thing containing zeaxanthin and/or its ester | |
| CN105199425A (en) | Extraction method of cyanidin | |
| CN103145871B (en) | The preparation method of cherry polysaccharide | |
| CN102899161B (en) | Process for extracting shrimp oil crude product from dried antarctic krill and treating foam | |
| CN105998091B (en) | A kind of extracting method and extract of Antrodia camphorata triterpene substance | |
| CN108822229A (en) | A kind of extracting method of lycium ruthenicum polysaccharide | |
| CN107050249A (en) | The extracting method of plant polyphenol in a kind of purple Chinese yam | |
| CN109060690B (en) | Method for detecting astaxanthin and structural analogue thereof in feed | |
| CN104432114A (en) | Method for extracting dietary fibres from passion fruit seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161207 |