CN106172010A - A kind of Cyclocarya paliurus Iljinskaja tissue cultured seedling root media and root culture method - Google Patents
A kind of Cyclocarya paliurus Iljinskaja tissue cultured seedling root media and root culture method Download PDFInfo
- Publication number
- CN106172010A CN106172010A CN201610692096.1A CN201610692096A CN106172010A CN 106172010 A CN106172010 A CN 106172010A CN 201610692096 A CN201610692096 A CN 201610692096A CN 106172010 A CN106172010 A CN 106172010A
- Authority
- CN
- China
- Prior art keywords
- root
- cultured seedling
- cyclocarya paliurus
- tissue cultured
- paliurus iljinskaja
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of Cyclocarya paliurus Iljinskaja tissue cultured seedling root media and root culture method.The formula of described root media is: improvement MS+IBA0.5～2.0mg/L+NAA 0～1.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/L+ agar 5～8g/L, pH5.5～6.0；Wherein, the formula of improvement MS is: a great number of elements: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L, potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；Organic compound: inositol 100mg/L, hydrochloric acid 1mg/L, pyridoxine hydrochloride 1mg/L, thiamine hydrochloride 0.2mg/L, glycine 2mg/L；Trace element is with MS culture medium.Above-mentioned culture medium carries out root culture, rooting rate >=70% to Cyclocarya paliurus Iljinskaja tissue cultured seedling.
Description
Technical field
The present invention relates to the tissue culture technique of plant, be specifically related to a kind of Cyclocarya paliurus Iljinskaja tissue cultured seedling root media and take root
Cultural method.
Background technology
It is blue or green that Cyclocarya paliurus Iljinskaja [Cyclocarya paliurus (Batal.) Iljinskaja] belongs to Juglandaceae (Juglandaceae)
Money sallow, another name money tree, fiber crops willow, blue or green money Lee, Boehmeria longispica Steud. willow, Shan Huashu.Cyclocarya paliurus Iljinskaja is described as " plant giant panda ", " doctor
3rd tree of educational circles ", it is the rare tree species that survives of glacier four discipline, exists only in China, be the in imminent danger of special-protection-by-the-State
Plant.Cyclocarya paliurus Iljinskaja is distributed mainly on the ground such as Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Taiwan, Hubei, Sichuan, Guizhou, Yunnan, many
Sporadicly it is distributed in remote, thickly forested mountains and some nature reserve areas.
Cyclocarya paliurus Iljinskaja has more health care and the medical value of uniqueness.Among the people do tea with leaf of Cyclocarya paliurus Iljinskaja for a long time, tea gas delicate fragrance,
Taste is sweet." Chinese medicine resource will want " (1994) are recorded, and leaf of Cyclocarya paliurus Iljinskaja tool heat clearing away is quenched one's thirst the effect of removing toxic substances." whole nation Chinese herbal medicine name mirror "
(1996) recording, Cyclocarya paliurus Iljinskaja bark, leaf, root have effect that killing parasites for relieving itching, anti-inflammatory analgetic dispel the wind.Modern pharmacology research proves,
Cyclocarya paliurus Iljinskaja has lowering blood pressure and blood fat and anti-ageing medicinal efficacy of waiting for a long time.Research find, leaf of Cyclocarya paliurus Iljinskaja contain substantial amounts of potassium, calcium, magnesium,
The macroelements such as phosphorus, possibly together with trace element such as manganese, ferrum, copper, chromium, zinc, selenium, vanadium, germanium.Wherein, make with carbohydrate metabolism and insulin
With closely-related elemental chromium, nickel, lithium, selenium, vanadium content higher, it is possible to assist insulin plays hypoglycemic activity, and can change
Kind carbohydrate tolerance.Leaf of Cyclocarya paliurus Iljinskaja possibly together with organic compound such as abundant polysaccharide, flavone, triterpene, steroid, saponin, coumarins, this
A little compounds can effectively reduce triglyceride and cholesterol, makes Cyclocarya paliurus Iljinskaja have the function of blood pressure lowering, blood sugar lowering, blood fat reducing, with
Time to strengthening body immunity, antioxidation, anti-ageing waiting for a long time have unique effect.
At present, the Cyclocarya paliurus Iljinskaja nursery stock on market mostly is wild resource transplanting or the seedling from seed of artificial culture.Due to green grass or young crops
Money willow kind source is limited, and the full rate of seed is low, also has deep dormancy characteristic, uses conventional seed planting method for culturing seedlings breeding cycle long, effect
Rate is low, it is difficult to produce a large amount of seedling in a short time, it is impossible to meet the demand in market.In recent years, domestic and international research worker with
In vitro Embryo, blade, tender shoots, stem section etc. in succession carry out the research of the tissue culture and rapid proliferation of Cyclocarya paliurus Iljinskaja for outer implant, as thanked
The third of the twelve Earthly Branches peak etc. (Xie Yinfeng etc. the preliminary study of Cyclocarya paliurus Iljinskaja tissue-culturing rapid propagation system. northwest Botany Gazette, 2009,29 (11): 2331-
2338) utilize In vitro Embryo tentatively to establish the rapid propagation system of Cyclocarya paliurus Iljinskaja, use WPM+0.2mg/L IBA+20g/L sucrose medium
Inducing, rooting rate is 16.67%, processes through 15d secretly induction, and rooting rate can bring up to 23.33%.Qiao Qingmei etc. are (tall
Minister in ancient times prunus mume (sieb.) sieb.et zucc., Cheng Maogao, Wei Zhihua. the impact on Cyclocarya paliurus Iljinskaja Jing Duan tissue culture of the different exogenous hormones. north gardening 2009 (7):
125-127) research finds, in Cyclocarya paliurus Iljinskaja Jing Duan tissue culture, the appropriate media of root induction is improvement MS+5.0mg/
L IBA, inductivity is 40.00%.Lu Meng etc. (Lu Meng etc. the Techniques of in Vitro Culture of Cyclocarya paliurus Iljinskaja stem segment with axillary buds. Nanjing Forestry University
Journal (natural science edition), 2013,37 (6): 6-10) with WPM as minimal medium, research IBA, GGR-6, ABT-1 are to blue or green money
The impact of Liu Shenggen, result shows, 10%GGR+1.0mg/L IBA combined treatment effect is best, and rooting rate is 25%.Existing
Result of study shows, Cyclocarya paliurus Iljinskaja tissue cultured seedling can success expanding propagation, but its rooting rate is low, quality of rooting is poor, it is difficult to meet batch production
The requirement of nursery.As can be seen here, the strengthening seedling and rooting technology of Cyclocarya paliurus Iljinskaja tissue cultured seedling has become restriction Cyclocarya paliurus Iljinskaja seedling factorial praluction
Key link, the breakthrough of tissue cultured seedling rooting technique, to set up Cyclocarya paliurus Iljinskaja efficient tissue-culturing rapid propagation system, expand planting scale have
Significance.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Cyclocarya paliurus Iljinskaja tissue cultured seedling root media and root culture method.
Use root media of the present invention that conventional Cyclocarya paliurus Iljinskaja tissue cultured seedling is carried out root culture, it is possible to obtain the life of more than 70%
Root rate, and root system development is good, plant strain growth is healthy and strong, and transplanting survival rate is high.
The formula of Cyclocarya paliurus Iljinskaja tissue cultured seedling root media of the present invention is:
Improvement MS+IBA0.5～2.0mg/L+NAA 0～1.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～
20g/L+ agar 5～8g/L, pH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L,
Potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, hydrochloric acid 1mg/L, pyridoxine hydrochloride 1mg/L, hydrochloric acid sulfur
Amine element 0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
In technique scheme, the formula of described Cyclocarya paliurus Iljinskaja root media is preferably: improvement MS+IBA0.8～1.2mg/
L+NAA0.2～0.8mg/L+ diethyl aminoethyl hexanoate 0.05～0.08mg/L+ sucrose 18～20g/L+ agar 5～8g/L, pH5.5～6.0；
Most preferably improvement MS+IBA1.0mg/L+NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L+ agar 5～8g/L,
PH5.5～6.0.The pH value of described Cyclocarya paliurus Iljinskaja root media is preferably 5.8～6.0.
Present invention additionally comprises a kind of root culture method of Cyclocarya paliurus Iljinskaja tissue cultured seedling, particularly as follows: cut individual plant tissue cultured seedling, access
Root media is cultivated at least 25d；Wherein:
The formula of described root media is:
Improvement MS+IBA0.5～2.0mg/L+NAA 0～1.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～
20g/L+ agar 5～8g/L, pH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L,
Potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, hydrochloric acid 1mg/L, pyridoxine hydrochloride 1mg/L, hydrochloric acid sulfur
Amine element 0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
In said method, the root culture condition of Cyclocarya paliurus Iljinskaja tissue cultured seedling is same as the prior art.It is preferably as follows: cultivation temperature
Being 20～30 DEG C, periodicity of illumination is 10～14h/d, and intensity of illumination is 2000～3000Lx；Further preferred condition of culture is:
Cultivation temperature is 23～27 DEG C, and periodicity of illumination is 10～12h/d, and intensity of illumination is 2000～3000Lx.
In said method, when Cyclocarya paliurus Iljinskaja tissue cultured seedling cultivates 25d in root media under above-mentioned condition of culture, tissue cultured seedling
Rooting rate i.e. can reach more than 70%, and root system development is good, and plant strain growth is healthy and strong.In the method for the invention, preferably
The root culture time of tissue cultured seedling is 25～35d.
In said method, the formula of described root media be preferably: improvement MS+IBA0.8～1.2mg/L+NAA0.2～
0.8mg/L+ diethyl aminoethyl hexanoate 0.05～0.08mg/L+ sucrose 18～20g/L+ agar 5～8g/L, pH5.5～6.0；Most preferably change
Good MS+IBA1.0mg/L+NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L+ agar 5～8g/L, pH5.5～6.0.Institute
The pH value stating Cyclocarya paliurus Iljinskaja root media is preferably 5.8～6.0.
In the method for the invention, obtain by existing conventional method isolated culture for carrying out the tissue cultured seedling of root culture,
Preferably with Cyclocarya paliurus Iljinskaja current-year branch, isolated culture gained according to a conventional method.Preferably choose the individual plant group training of height >=2cm
Seedling carries out root culture, more preferably chooses height >=3cm and length has the individual plant tissue cultured seedling of 3～4 compound leaves to carry out root culture,
To improve the transplanting survival rate of plant.
Compared with prior art, present invention is characterized in that by improvement minimal medium constituent concentration, be used in combination spy
Determine the diethyl aminoethyl hexanoate of concentration, IBA and NAA plant growth regulator, make gained root media can effectively facilitate the training of Cyclocarya paliurus Iljinskaja group
Taking root of Seedling.Utilize this root media that Cyclocarya paliurus Iljinskaja tissue cultured seedling is carried out root culture, rooting rate can be made to reach more than 70% (excellent
Select embodiment up to 82%), and root system development is good, plant strain growth is healthy and strong, and transplanting survival rate is high, produces for realizing the training of Cyclocarya paliurus Iljinskaja group
Industry and popularization and application provide technical support.
Accompanying drawing explanation
Fig. 1 is the picture of the Cyclocarya paliurus Iljinskaja tissue cultured seedling obtained according to a conventional method in embodiment 1；
Fig. 2 is to use the root media described in the embodiment of the present invention 1 to take root when cultivating 35d the picture in shoot root portion.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, to be more fully understood that present disclosure, but
The present invention is not limited to following example.
In following embodiment, involved improvement MS includes a great number of elements, trace element and organic compound, concrete
Formula is as follows:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L,
Potassium dihydrogen phosphate 60mg/L, calcium chloride dihydrate 165mg/L；
The component of trace element and concentration is: potassium iodide 0.83mg/L, boric acid 6.2mg/L, four water manganese sulfate 22.3mg/L,
Zinc sulphate heptahydrate 8.6mg/L, Sodium Molybdate Dihydrate 0.25mg/L, copper sulphate pentahydrate 0.025mg/L, cobalt chloride hexahydrate 0.025mg/L,
Ferrous sulfate heptahydrate 27.8mg/L.Wherein, ferrous sulfate heptahydrate chelates with disodiumedetate, disodiumedetate
Concentration is 37.3mg/L.
The component of organic compound and concentration is: inositol 100mg/L, nicotinic acid 1mg/L, pyridoxine hydrochloride 1mg/L, hydrochloric acid sulfur
Amine element 0.2mg/L, glycine 2mg/L.
Embodiment 1
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains tissue cultured seedling according to a conventional method, as shown in Figure 1；
2) root culture: cut higher than 3.5cm and the long individual plant tissue cultured seedling having 3～4 compound leaves, access in root media
Cultivate 35d (picture in shoot root portion of taking root when cultivating 35d is as shown in Figure 2)；Wherein:
The formula of described root media is: improvement MS+IBA1.0mg/L+NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sugarcane
Sugar 20g/L+ agar 7g/L, pH5.8.
Root culture condition is: temperature is 23～25 DEG C, and periodicity of illumination is 12h/d, and intensity of illumination is 2000Lx.
By strain number of taking root/inoculation strain number × 100% to calculate rooting rate (following identical), rooting rate is 82%.
Comparative example 1
The method repeating embodiment 1, is simply revised as prescription of rooting medium: improvement MS+IBA1.0mg/L+
NAA0.5mg/L+ sucrose 20g/L+ agar 7g/L, pH5.8.
Being computed, rooting rate is 30%.
Comparative example 2
The method repeating embodiment 1, is simply revised as prescription of rooting medium: improvement MS+IBA1.0mg/L+
NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.01mg/L+ sucrose 20g/L+ agar 7g/L, pH5.8.
Being computed, rooting rate is 40%.
Comparative example 3
The method repeating embodiment 1, is simply revised as prescription of rooting medium: improvement MS+IBA1.0mg/L+
NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.2mg/L+ sucrose 20g/L+ agar 7g/L, pH5.8.
Being computed, rooting rate is 0.
Embodiment 2
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains tissue cultured seedling according to a conventional method；
2) root culture: cut higher than 2.5cm and the long individual plant tissue cultured seedling having 2～3 compound leaves, access in root media
Cultivate 30d；Wherein:
The formula of described root media is: improvement MS+IBA2.0mg/L+ diethyl aminoethyl hexanoate 0.04mg/L+ sucrose 15g/L+ fine jade
Fat 7g/L, pH6.0.
Condition of culture in root media is: temperature is 25 DEG C, and periodicity of illumination is 10h/d, and intensity of illumination is
3000Lx。
Being computed, rooting rate is 80%.
Embodiment 3
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains tissue cultured seedling according to a conventional method；
2) root culture: cut higher than 3cm and the long individual plant tissue cultured seedling having 2～3 compound leaves, access in root media and train
Support 28d；Wherein:
The formula of described root media is: improvement MS+IBA1.5mg/L+NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.02mg/L+ sugarcane
Sugar 18g/L+ agar 5.5g/L, pH6.0；
Condition of culture in root media is: temperature is 27 DEG C, and periodicity of illumination is 10h/d, and intensity of illumination is
2800Lx。
Being computed, rooting rate is 72%.
Embodiment 4
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains tissue cultured seedling according to a conventional method；
2) root culture: choose height more than 2cm and the long individual plant tissue cultured seedling having 1～2 compound leaf, access root media
Middle cultivation 25d；Wherein:
The formula of described root media is: improvement MS+IBA1.5mg/L+NAA0.2mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sugarcane
Sugar 18g/L+ agar 5g/L, pH5.5.
Condition of culture in root media is: temperature is 20 DEG C, and periodicity of illumination is 14h/d, and intensity of illumination is
2500Lx。
Being computed, rooting rate is 80%.
Embodiment 5
1) taking Cyclocarya paliurus Iljinskaja current-year branch, isolated culture obtains tissue cultured seedling according to a conventional method；
2) root culture: cut higher than 3.5cm and the long individual plant tissue cultured seedling having 3～4 compound leaves, cut the wound healing group of base portion
Knit, then access and root media is cultivated 27d；Wherein:
The formula of described root media is: improvement MS+IBA0.8mg/L+NAA0.8mg/L+ diethyl aminoethyl hexanoate 0.1mg/L+ sugarcane
Sugar 15g/L+ agar 8g/L, pH5.9.
Condition of culture in root media is: temperature is 30 DEG C, and periodicity of illumination is 10h/d, and intensity of illumination is 2000
～2200Lx.
Being computed, rooting rate is 70%.
Claims (7)
1. a Cyclocarya paliurus Iljinskaja tissue cultured seedling root media, it is characterised in that: the formula of this root media is:
Improvement MS+IBA0.5～2.0mg/L+NAA 0～1.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/L+
Agar 5～8g/L, pH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L, phosphoric acid
Potassium dihydrogen 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, hydrochloric acid 1mg/L, pyridoxine hydrochloride 1mg/L, thiamine hydrochloride
0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
Cyclocarya paliurus Iljinskaja root media the most according to claim 1, it is characterised in that: the formula of described root media is:
Improvement MS+IBA1.0mg/L+NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L+ agar 5～8g/L, pH5.5～
6.0。
3. the root culture method of a Cyclocarya paliurus Iljinskaja tissue cultured seedling, it is characterised in that: cut individual plant tissue cultured seedling, access root media
Middle cultivation at least 25d；Wherein:
The formula of described root media is:
Improvement MS+IBA0.5～2.0mg/L+NAA0～1.0mg/L+ diethyl aminoethyl hexanoate 0.02～0.1mg/L+ sucrose 15～20g/L+ fine jade
Fat 5～8g/L, pH5.5～6.0；
Wherein, the formula of improvement MS is:
The component of a great number of elements and concentration is: potassium nitrate 700mg/L, ammonium nitrate 600mg/L, Magnesium sulfate heptahydrate 185mg/L, phosphoric acid
Potassium dihydrogen 60mg/L, calcium chloride dihydrate 165mg/L；
The component of organic compound and concentration is: inositol 100mg/L, hydrochloric acid 1mg/L, pyridoxine hydrochloride 1mg/L, thiamine hydrochloride
0.2mg/L, glycine 2mg/L；
The component of trace element and concentration are with MS culture medium.
The root culture method of Cyclocarya paliurus Iljinskaja tissue cultured seedling the most according to claim 3, it is characterised in that: the training of taking root of tissue cultured seedling
Foster condition is: cultivation temperature is 20～30 DEG C, and periodicity of illumination is 10～14h/d, and intensity of illumination is 2000～3000Lx.
The root culture method of Cyclocarya paliurus Iljinskaja tissue cultured seedling the most according to claim 4, it is characterised in that: the training of taking root of tissue cultured seedling
Foster condition is: cultivation temperature is 23～27 DEG C, and periodicity of illumination is 10～12h/d, and intensity of illumination is 2000～3000Lx.
6. according to the root culture method of the Cyclocarya paliurus Iljinskaja tissue cultured seedling according to any one of claim 3～5, it is characterised in that: group
The root culture time of seedlings cultivating is 25～35d.
7. according to the root culture method of the Cyclocarya paliurus Iljinskaja tissue cultured seedling according to any one of claim 3～5, it is characterised in that: institute
The formula stating root media is:
Improvement MS+IBA1.0mg/L+NAA0.5mg/L+ diethyl aminoethyl hexanoate 0.05mg/L+ sucrose 20g/L+ agar 5～8g/L, pH5.5～
6.0。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610692096.1A CN106172010B (en) | 2016-08-17 | 2016-08-17 | A kind of blue or green money willow tissue-cultured seedling root media and culture of rootage method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610692096.1A CN106172010B (en) | 2016-08-17 | 2016-08-17 | A kind of blue or green money willow tissue-cultured seedling root media and culture of rootage method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106172010A true CN106172010A (en) | 2016-12-07 |
| CN106172010B CN106172010B (en) | 2018-05-11 |
Family
ID=57523046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610692096.1A Active CN106172010B (en) | 2016-08-17 | 2016-08-17 | A kind of blue or green money willow tissue-cultured seedling root media and culture of rootage method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106172010B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105259A (en) * | 2018-08-16 | 2019-01-01 | 中南林业科技大学 | A kind of method of blue or green money willow tissue-cultured seedling fast breeding |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487819A (en) * | 2011-11-22 | 2012-06-13 | 嘉兴浙华农业技术开发有限公司 | Cyclocarya paliurus callus and suspension culture method for callus particles |
| CN105052745A (en) * | 2015-08-24 | 2015-11-18 | 华南农业大学 | Method for promoting growth of echinacea purpurea regeneration buds |
-
2016
- 2016-08-17 CN CN201610692096.1A patent/CN106172010B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487819A (en) * | 2011-11-22 | 2012-06-13 | 嘉兴浙华农业技术开发有限公司 | Cyclocarya paliurus callus and suspension culture method for callus particles |
| CN105052745A (en) * | 2015-08-24 | 2015-11-18 | 华南农业大学 | Method for promoting growth of echinacea purpurea regeneration buds |
Non-Patent Citations (4)
| Title |
|---|
| 胡海涛等: "余甘子下胚轴离体再生体系的优化", 《园艺学报》 * |
| 谢寅峰等: "青钱柳子叶不定根的发生机制", 《林业科学》 * |
| 鲁萌等: "青钱柳茎段腋芽的离体培养技术", 《南京林业大学学报(自然科学版)》 * |
| 龚峥等: "枫香组织培养快繁育苗技术研究", 《广东林业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105259A (en) * | 2018-08-16 | 2019-01-01 | 中南林业科技大学 | A kind of method of blue or green money willow tissue-cultured seedling fast breeding |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106172010B (en) | 2018-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105052655B (en) | Oil tea implantation methods | |
| CN104472359B (en) | A kind of ginseng adventitious root proliferative induction method | |
| CN103651121B (en) | A kind of bletilla differentiation, strong seedling culture base | |
| CN102630562B (en) | Rapid propagation method of Jiuhua Polygonatum sibiricum | |
| CN104496625B (en) | A kind of nutrient solution prescription of blueberry Water culture | |
| CN104285813B (en) | Camellia chrysantha tissue culture propagation method | |
| CN104273028B (en) | Method for rapid in-vitro propagation of Crassulaceae plant | |
| CN103651122B (en) | A kind of bletilla protocorm induction medium | |
| CN106171978B (en) | A kind of tissue culture and rapid propagation method of P. kingianum | |
| CN104429938A (en) | Tissue culture method for dendrobium officinale kiura et migo | |
| CN107864862B (en) | Method for quickly and efficiently obtaining tissue culture seedlings of ceiba | |
| CN104542306A (en) | Fast propagation method of longans | |
| Pant et al. | Various culture techniques for the mass propagation of medicinal orchids from Nepal | |
| CN103039369A (en) | In-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium | |
| CN109819892B (en) | Tissue culture method of good single plant of tsaoko | |
| CN103168693A (en) | Proliferation culture medium for aristolochia longgonensis tissue culture and application of proliferation culture medium | |
| CN107148883A (en) | A kind of oil tea implantation methods | |
| CN106106185B (en) | A kind of blue or green money willow rooting of vitro seedling culture medium and culture of rootage method | |
| CN106172010B (en) | A kind of blue or green money willow tissue-cultured seedling root media and culture of rootage method | |
| CN102823491B (en) | Method for culturing horseradish callus through taking horseradish root as explant | |
| CN107278562A (en) | A kind of cuttage and seedling culture method of tea tree | |
| CN103621405A (en) | Artificial corychophramus violaceua seed making method | |
| CN110214694A (en) | Zhejiang hymsleya amabilis is female, staminiferous plant quick breeding by group culture method | |
| CN106106186B (en) | A kind of root media of blue or green money willow test tube seedling and the tissue culture and rapid propagation method of blue or green money willow | |
| CN106106184B (en) | Blue or green money willow root media and its cultural method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |