CN106171898A - A kind of method of seedling stage assay Semen arachidis hypogaeae black rot resistant variety - Google Patents
A kind of method of seedling stage assay Semen arachidis hypogaeae black rot resistant variety Download PDFInfo
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- CN106171898A CN106171898A CN201610589908.XA CN201610589908A CN106171898A CN 106171898 A CN106171898 A CN 106171898A CN 201610589908 A CN201610589908 A CN 201610589908A CN 106171898 A CN106171898 A CN 106171898A
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- arachidis hypogaeae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention belongs to agricultural technology field, a kind of method specifically disclosing seedling stage assay Semen arachidis hypogaeae black rot resistant variety, it it is the Semen arachidis hypogaeae black rot hyphal suspension immersion Semen arachidis hypogaeae plumelet using 0.5～5%, after soaking a period of time, moisturizing 2 d carries out water planting cultivation again, the method i.e. using indoor inoculation to identify, can identify the resistance of peanut varieties seedling stage.The method is easy, efficient, and acquired results good stability, repeatability are high, can be that the Field information of Semen arachidis hypogaeae black rot breeding for disease resistance and kind provides theoretical foundation.
Description
Technical field
The present invention relates to agricultural technology field, more particularly, to a kind of seedling stage assay Semen arachidis hypogaeae black rot resistant variety
Method.
Background technology
Semen arachidis hypogaeae black rot (Cylindrocladium black rot of peanut is called for short CBR) is that one has a strong impact on
The important disease of peanut production.Semen arachidis hypogaeae black rot infecting peanut underground organization fruit pin, basal part of stem, root system and fruit pod etc., infect
The susceptible rear blackening in position is rotted, and when falling ill serious, all blade tables of plant reveal the most even wilting symptoms that withers, last whole plant
Withered death.Up to now, external report occurs the country of Semen arachidis hypogaeae black rot to have the U.S., Brazil, Japan, Korea S, Korea, print
Degree, Iran, Cameroon, Australia etc..2009, China Guangdong found Semen arachidis hypogaeae black rot first, and subsequently, Fujian, Jiangxi are equal
There is this disease in report.
Disease-resistant variety cultivation is one of emphasis of Semen arachidis hypogaeae black rot study on prevention.Natural induction in fields is identified and greenhouse is artificial
Inoculated identification is two kinds of effective ways of current peanut varieties Resistance Identification.Natural induction in fields is test to be built up in field send out
Lesion, and carry out under the natural conditions of beneficially plant disease epidemic.The advantage of the method is can to reflect that kind is to disease truly
Field resistance performance.But, field affects a lot, such as Microsclerotia quantity, soil in soil because have of Semen arachidis hypogaeae black rot generation
Earth fertility, weather conditions etc., therefore the result stability of the method is the highest, and test repeatability is poor；Greenhouse artificial vaccination
Resistance Identification, is to carry out Resistance Identification by artificial vaccination in the greenhouse that humiture, illumination are the most controlled, owing to condition is controlled,
The shortcoming that the method can overcome natural induction in fields Resistance Identification result poor repeatability.Semen arachidis hypogaeae black rot Resistance Identification artificial vaccination
Method mainly has conidial suspension root-pouring method, Microsclerotia or kernel culture to mix soil inocalation method.2008, Dong used germ
Core mix soil inocalation method carry out Semen arachidis hypogaeae black rot Resistance Identification, have studied Microsclerotia size, every gram of soil Microsclerotia quantity this two
The impact that individual factor antagonism is identified, result shows, is 5/g and 10/g in concentration, 250mm Microsclerotia < during 425mm,
The rhizome of disease-resistant variety Georgia-02C rots serious, does not show disease resistance.2011, Deng Qiaowen compared mitogenetic spore
Sub-pouring root, Microsclerotia mix soil and kernel culture mixes the effect of three kinds of inoculation methods of soil, found that kernel culture mixes soil inocalation method
Simply, conveniently, effective, experimental result can be drawn after inoculating 35 days.
For the preventing and treating of Semen arachidis hypogaeae black rot, presently preferred way is breeding resistant variety.Owing to this disease is near in China
Within several years, just finding, disease related basic research is weaker, there is no the evaluation of resistance of existing peanut varieties and germ plasm resource and is
System research.
Summary of the invention
The technical problem to be solved is the drawbacks described above overcoming prior art to exist, it is provided that a kind of seedling stage assay
The method of Semen arachidis hypogaeae black rot resistant variety.
Second object of the present invention is to provide said method application in peanut breeding.
It is an object of the invention to be achieved by the following technical programs:
A kind of method of seedling stage assay Semen arachidis hypogaeae black rot resistant variety, is employing 0.5～the Semen arachidis hypogaeae black rot mycelia of 5%
Suspension soaks Semen arachidis hypogaeae plumelet, and after soaking a period of time, moisturizing 2d carries out water planting cultivation again.
After research finds the immersion of Semen arachidis hypogaeae black rot hyphal suspension, moisturizing is the heaviest for the dip-dye of Semen arachidis hypogaeae black rot
Want, therefore, when seed resistance is identified, need, after soaking Semen arachidis hypogaeae plumelet, to carry out the moisturizing of a period of time, then carry out
Water planting is cultivated, and is greatly improved determination rates and the accuracy rate of Semen arachidis hypogaeae resistant variety in seedling stage.
China's Semen arachidis hypogaeae Type division is plain edition, Pearl Type, many types, the raw type of dragon and middle type five class, and abroad divides
For Spanish-type, Virginia type, Valencia type.The research of foreign scholar shows, Spanish-type (is equivalent to China's pearl beans
Type) Semen arachidis hypogaeae is the strongest to Semen arachidis hypogaeae black rot resistance, and secondly, and Wahlen is western for Virginia type (being equivalent to China's plain edition) Semen arachidis hypogaeae resistance
Hypotype (being equivalent to many types of China) Semen arachidis hypogaeae the most susceptible (Bell et al., 1973；Hammons et al.,1979).This research
Result show, the kind of high anti-Semen arachidis hypogaeae black rot or material famine, the material that performance height is anti-in 128 parts of materials is only
1, i.e. belonging to medium-sized T09, the material of performance medium resistance mostly is Pearl Type, and shows existing many of the kind of high sense
The Flos Osmanthi Fragrantis of type No. 35, also has the P562 of Pearl Type, and therefore resistance and the type belonging to it of black rot be there is no directly by peanut varieties
Connect dependency.
It is conventional culture technique that water planting of the present invention is cultivated, it is preferable that be at 25 DEG C of illumination cultivation indoor cultivation, often
Changed 1 water every 3 days, plant can be extracted after 10d, identify according to the incidence of plant.
After the present invention adds black rot hyphal suspension in peanut seedling, soak time is for Semen arachidis hypogaeae black rot
Dip-dye effect is not significantly different from, and general immersion 10～30min is advisable.
Preferably, described Semen arachidis hypogaeae plumelet is by being soaked by peanut seed, and the radicle 1 obtained in 30 DEG C of black dull cultivation 2d
～the plumelet of 2cm length.
It is highly preferred that in said method, the concentration of described Semen arachidis hypogaeae black rot hyphal suspension is 0.5～1%；Most preferably
Ground, the concentration of described Semen arachidis hypogaeae black rot hyphal suspension is 0.5%.
The present invention also provides for the resistance that the method for described seedling stage assay Semen arachidis hypogaeae black rot resistant variety is studied at peanut breeding
Application in qualification；Preferably, it is that the method utilizing described seedling stage assay Semen arachidis hypogaeae black rot resistant variety is to peanut breeding material
Make the application in batch screening.
Compared with prior art, the method have the advantages that
A kind of method that the invention provides seedling stage assay Semen arachidis hypogaeae black rot resistant variety, is employing 0.5～the Semen arachidis hypogaeae of 5%
Black rot hyphal suspension soaks Semen arachidis hypogaeae plumelet, and after soaking a period of time, moisturizing 2d carries out water planting cultivation again, i.e. uses indoor
The method of inoculated identification, can identify the resistance of peanut varieties seedling stage.The method is easy, efficient, acquired results good stability, weight
Renaturation is high, can be that the Field information of Semen arachidis hypogaeae black rot breeding for disease resistance and kind provides theoretical foundation.
Accompanying drawing explanation
Fig. 1 is Semen arachidis hypogaeae black rot 0～5 grades of root rot symptoms.
Fig. 2 is the disease resistance performance of T09；Wherein a～c is that inoculation processes；D is comparison；E compares with inoculation for comparison；F is
Comparison root system；G is that inoculation processes root system.
Fig. 3 is the disease resistance performance of P562: wherein a～c is that inoculation processes；D is comparison；E is comparison root system；F is inoculation
Process root system.
Detailed description of the invention
Further illustrate present disclosure below in conjunction with Figure of description and specific embodiment, but should not be construed as this
The restriction of invention.Without departing from the spirit and substance of the case in the present invention, that is made the inventive method, step or condition is simple
Amendment or replacement, belong to the scope of the present invention；If not specializing, technological means used in embodiment is art technology
Conventional means known to personnel.
Embodiment 1
With the red peanut varieties in four, Fuyu County (purchased from San Jingzi town, Fuyu County, Jilin Province Xing Ku treasured Semen arachidis hypogaeae trading section) as material,
Carry out inoculation condition test.Specific as follows:
1, prepared by hyphal suspension
The Semen arachidis hypogaeae black rot that 4 DEG C preserve is inoculated on PDA culture medium flat plate, cultivates in 25 DEG C of constant incubators
5d.Cut 10 truffles silk blocks with the card punch of a diameter of 1cm, put in the 300mL conical flask equipped with 150mL PD culture fluid, put
In 25 DEG C, shaken cultivation 5d in the constant-temperature table of 180r/min.By four layers of filtered through gauze bacterium solution after sterilizing, choose residual agar
Block, the mycelia on scraping gauze, mycelia is placed on aseptic filter paper extruding suck dry moisture, weighs 5.0g mycelia and add 95mL 4 DEG C
In sterilized water, it is homogenized 2min.Hyphal suspension after homogenate is carried out microscopy, it is ensured that without more than 1mm in the range of field of microscope
Hyphal body；Hyphal suspension is now with the current.
2, peanut seed accelerating germination
Choose healthy full, without the peanut seed 50 of disease pest～60, rinse well with tap water, put in culture dish,
Add water not peanut seed, culture dish is put into black dull accelerating germination 2d in 30 DEG C of calorstats；Change a water every 12h, change water every time
Rinse more than 3 times with tap water.Choose and sprout normal, the plumelet of radicle 1～2cm length, standby.
3, inoculation condition is selected
The method using hyphal suspension to soak plumelet inoculation is tested.The concentration of inoculation hyphal suspension sets 5%,
3%, 1% (mass/volume, the grams of the mycelia contained in i.e. every 100mL water) totally 3 Concentraton gradient, with sterilized water for comparison；
Soak time sets 10min, 20min and 30min tri-kinds process；Inoculation post processing sets and is inserted directly into water planting cotton (direct water planting process)
Two kinds of process are cultivated with inserting water planting cotton after moisturizing 2d.Often process 3 times and repeat, each repetition 5 strain.The water of inoculation plumelet will be inserted with
Training cotton is put in the pallet filling with water, cultivates, changed 1 water every 3 days, extract plant after 10d in 25 DEG C of illumination cultivation rooms, right
Observe the root that every ZHUHUA is raw, record root-rot grade and morbidity strain number according to grade scale, calculate disease index, compare different vaccination
The effect of inoculation of condition, selected inoculation soak time and inoculation post-processing approach.
4, grade scale is described as follows:
0 grade: there is no symptom；
1 grade: the slight browning of basal part of stem；
2 grades: basal part of stem blackening, main root blackening length is less than 50%；
3 grades: basal part of stem blackening, main root blackening length is more than 50%, with the presence of a little secondary root；
4 grades: the complete blackening of main root also starts to decay, and secondary root rots to drop；
5 grades: the complete blackening of main root is rotted, plant is wilted even dead.
Peanut varieties resistance Rapid identification standard is based on disease index grade.
Disease index=∑ (morbidity strain numbers at different levels × at different levels represent numerical value)/(investigation sum × the highest disease index) ×
100
Immunity: disease index=0.
High anti-: 0 < disease index 10.
In anti-: 10 < disease index 20.
Middle sense: 20 < disease index 30.
High sense: disease index > 30.
The red peanut varieties in four, Fuyu County, at 3 hyphal suspension inoculum densities for examination, under 3 immersion treatment times, soaks
After bubble inoculation, average attack rate and the disease index of moisturizing 2d are above soaking direct water planting after inoculating and process.Illustrate that mycelia suspends
After immersion bubble, moisturizing infects particularly significant (table 1) for Semen arachidis hypogaeae black rot.Inoculum density and soak time disease index
Two-way analysis of variance result shows (be shown in Table 2, table 3), after inoculation in the case of moisturizing 2d water planting, between different soak times
Difference notable, and the significant difference between inoculum density；After inoculation in the case of direct water planting, during different immersion inoculation
Difference between is not notable, and the difference between inoculum density is the most notable.Between the immersion inoculation time of two kinds of processing methods
Difference is not the most notable, illustrates to have no significant effect peanut varieties disease index for the immersion inoculation time of examination, therefore, and varietal resistance
Identify with moisturizing 2d water culture after inoculation preferable, soak inoculation time and be advisable with 10min.
The red incidence in different vaccination condition in four, table 1 Fuyu County
Note: sickness rate and disease index in table are the average repeated 3 times.
The disease index two-way analysis of variance of 2 days water plantings of moisturizing after table 2 inoculation
| Difference is originated | SS | df | MS | F | P-value | F 0.05 |
| Time | 19.36889 | 2 | 9.684444 | 1.990182 | 0.251232 | 6.944272 |
| Concentration | 171.3089 | 2 | 85.65444 | 17.60224 | 0.01041 | 6.944272 |
| Error | 19.46444 | 4 | 4.866111 | |||
| Amount to | 210.1422 | 8 |
The disease index two-way analysis of variance of direct water planting after table 3 inoculation
| Difference is originated | SS | df | MS | F | P-value | F 0.05 |
| Time | 16.34889 | 2 | 8.174444 | 0.146357 | 0.868273 | 6.944272 |
| Concentration | 6.295556 | 2 | 3.147778 | 0.056358 | 0.945937 | 6.944272 |
| Error | 223.4111 | 4 | 55.85278 | |||
| Amount to | 246.0556 | 8 |
Use hyphal suspension to soak plumelet inocalation method, set immersion inoculation time as 10min, moisturizing 2d water again after inoculation
Training, arranges 1%, 0.5% and 0.1% 3 inoculum density, compares the disease index of different vaccination concentration, and selected varietal resistance is commented
The inoculum density of valency test.Result shows, along with the rising of inoculum density, disease index raises accordingly, but is all higher than 30, right
The impact of varietal resistance grade classification is little (being shown in Table 4).Concentration one factor analysis of variance result shows, poor between 3 inoculum densities
Different notable (F=12.11 > F0.05) (being shown in Table 5), illustrates that different vaccination concentration has more apparent impact to disease index.For ensureing to connect
Kind of effect, makes quantity of microorganism inoculated try one's best access expansion level simultaneously, selects the inoculum density that 0.5% is varietal resistance evaluation.
The red incidence under different vaccination concentration in four, table 4 Fuyu County
| Inoculum density (%) | Sickness rate (%) | Disease index |
| 0 | 0 | 0 |
| 0.1 | 100 | 33.3 |
| 0.5 | 100 | 45.3 |
| 1 | 100 | 56 |
Note: sickness rate and disease index in table are the average repeated 3 times, and soak time is 10min, inoculates post processing
For moisturizing 2d water planting.
The disease index variance analysis of table 5 different vaccination concentration
Embodiment 2
It is material with 128 peanut varieties (being provided by ZhongKai Agriculture Engineering Academy professor Zheng Yixiong, be specifically shown in Table 6), connects
Planting hyphal suspension concentration is 0.5% (mass/volume), and soak time is 10min, with sterilized water for comparison, carries out kind and resists
Characteristic of disease is evaluated.Each kind repeats for 3 times, each repetition 10 strain.After soaking inoculation, Semen arachidis hypogaeae plumelet is put into and be lined with double-deck wet gauze
Pallet in, cover with double-deck wet gauze, again Semen arachidis hypogaeae plumelet inserted in water planting silk floss after 25 DEG C of constant-temperature moisture-keeping 2d, be placed in 25 DEG C
Illumination cultivation room is cultivated.Extracting plant after 10d, comparison grade scale observes root, record root-rot grade and morbidity strain number, meter
Calculate disease index, evaluate experimental cultivar or the disease resistance of material.
128 experimental cultivars or the average root-rot grade of material, sickness rate, disease index statistical result are as shown in table 6.Table
The result of 6 shows, in 128 experimental cultivars or material, the disease index kind more than 30 has 72, and disease index is 20～30
Kind have 42, disease index is that 10～20 kinds have 13, disease index be 0～10 kind have 1, disease index is
The kind of zero is 0.It is to say, 128 peanut varieties to the performance immunity of Semen arachidis hypogaeae black rot, high anti-, in anti-, middle sense, high sense
Kind have 0,1,13,42 and 72 respectively, account for respectively for examination the 0% of peanut varieties, 0.8%, 10.2%,
32.7% and 56.3%.Wherein, the disease index of T09 is minimum, is 8.0, and sickness rate is 36.7%, at 128 experimental cultivars or
In material the most disease-resistant (Fig. 2)；The disease index of P562 is the highest, is 46.0, sickness rate 100%, at 128 experimental cultivars or material
In the most susceptible (Fig. 3).
The state of an illness statistics of 6 128 peanut varieties of table or material
Continued 6 (1)
| Kind or material | Sickness rate (%) | Disease index | Kind or material | Sickness rate (%) | Disease index |
| The profound autumn 48 | 100 | 38.6±0.33ab | S65 | 83.3 | 20.0±0.0cd |
| Profound oil 15 | 100 | 38.7±0.46ab | Z55 | 96.7 | 28.6±0.29c |
| ZR02 | 100 | 35.2±0.43b | H16 | 100 | 29.0±0.49cb |
| Million caul-fats 1 | 100 | 38.5±0.26ab | ZH16 | 100 | 33.3±1.22b |
| Bight kind | 100 | 34.4±0.49b | M18 | 50 | 12.0±0.30d |
| Congratulate oil 15 | 100 | 34.6±0.48b | M21 | 96.6 | 22.6±0.81c |
| Dragon spends 18 | 100 | 31.0±0.46b | ML23 | 83.3 | 20.6±0.36c |
| G69 | 100 | 29.7±0.35cb | ZK09 | 100 | 37.6±0.46b |
| G99 | 100 | 38.0±0.29ab | The big fruit in Huaiji | 100 | 32.0±0.90b |
| G127 | 100 | 29.6±0.23cb | AS09 | 87.7 | 20.0±0.31d |
| G267 | 95 | 36.0±0.40b | BS3 | 83.3 | 19.3±0.12d |
| G403 | 100 | 33.5±0.55b | DR1 | 100 | 20.7±0.55cd |
| G421 | 100 | 36.0±0.23b | G106 | 100 | 21.3±0.40c |
| G836 | 100 | 38.5±0.20ab | G771 | 90 | 22.0±0.32c |
| GH02 | 100 | 35.0±0.40b | G833 | 100 | 23.3±0.69c |
| GH10 | 100 | 31.0±0.25b | GH1127 | 100 | 22.7±0.56c |
| GH12 | 100 | 33.8±0.58b | GR95 | 100 | 28.7±0.87c |
| GH15 | 100 | 35.0±0.67b | GR166 | 100 | 24.0±0.87c |
| GH1409 | 100 | 39.0±0.32ab | H22 | 100 | 30.0±0.32b |
| H17 | 100 | 38.5±0.26ab | HB1 | 100 | 25.3±0.46c |
| H21 | 100 | 40.0±0.28a | HB2 | 100 | 26.0±0.75c |
| H23 | 100 | 42.1±0.40a | HG126 | 73.3 | 17.3±0.55d |
| H126 | 100 | 38.0±0.65ab | KQ29 | 100 | 25.7±0.61c |
Continued 6 (2)
| Kind or material | Sickness rate (%) | Disease index | Kind or material | Sickness rate (%) | Disease index |
| H716 | 100 | 35.0±0.55b | M10 | 100 | 27.3±0.75c |
| HC1 | 100 | 36.9±0.32b | M1012 | 100 | 32.0±0.61b |
| J02 | 100 | 37.7±0.45b | P559 | 93.3 | 21.3±0.75c |
| JG08 | 100 | 38.0±0.20ab | Q07 | 100 | 22.0±0.61c |
| L106 | 100 | 40.0±0.28a | Q08 | 100 | 29.3±0.25cb |
| LYX | 100 | 35.3±0.37b | Q509 | 93.3 | 19.3±0.45d |
| Q506 | 82 | 22.7±1.08c | Z41 | 100 | 31.3±0.17b |
| Q646 | 100 | 26.0±0.36c | Z93 | 100 | 20.0±0.80d |
| S188 | 100 | 40.0±0.51a | ZK03 | 83.3 | 18.0±0.60d |
| S217 | 100 | 38.0±0.64b | Colored pearl bean | 100 | 24.7±0.98c |
| T03 | 100 | 36.0±0.55b | Flos Osmanthi Fragrantis 35 | 100 | 35.3±0.52b |
| T228 | 100 | 38.0±0.80ab | Flower Xiaobao | 60 | 12.0±0.62d |
| Z75 | 100 | 36.7±0.98b | Spring spends 557 | 100 | 27.3±0.64c |
| Z82 | 100 | 35.8±0.75b | Shan oil red | 90 | 23.3±0.40c |
| M05 | 96.4 | 31.4±0.70b | Q510 | 87.7 | 28.0±0.52c |
| ZK332 | 100 | 32.0±0.30b | Shan oil red 1 | 100 | 25.3±0.90c |
| ZK02 | 100 | 38.0±0.42b | Shan oil lures No. 2 | 100 | 25.3±0.33c |
| ZK06 | 100 | 37.3±0.60b | Cloud Semen arachidis hypogaeae | 93.3 | 25.3±0.39c |
| ZK07 | 100 | 38.4±0.55ab | Megastream is little red | 73.3 | 22.7±1.3c |
| ZK08 | 100 | 38.0±0.92ab | E002 | 100 | 29.4±0.17c |
| ZK11 | 100 | 36.3±0.55b | GH14 | 89.4 | 23.1±1.04c |
| Human relations three water | 100 | 39.0±0.32ab | ML09 | 100 | 24.7±0.69c |
| M06 | 100 | 36.5±0.91b | Q511 | 87.7 | 18.0±0.80d |
Continued 6 (3)
Note: in table, data are the meansigma methods repeated 3 times, and the upper right corner indicates different the English alphabets and shows significant difference (P
<0.05)。
Claims (6)
1. the method for a seedling stage assay Semen arachidis hypogaeae black rot resistant variety, it is characterised in that employing 0.5～the Semen arachidis hypogaeae black rot of 5%
Bacterium hyphal suspension soaks Semen arachidis hypogaeae plumelet, and after soaking a period of time, moisturizing 2 d carries out water planting cultivation again.
The method of seedling stage assay Semen arachidis hypogaeae black rot resistant variety the most according to claim 1, it is characterised in that described Semen arachidis hypogaeae
The concentration of black rot hyphal suspension is 0.5～1%.
The method of seedling stage assay Semen arachidis hypogaeae black rot resistant variety the most according to claim 1, it is characterised in that described flower
Raw plumelet is by being soaked by peanut seed, and the plumelet of the radicle 1～2 cm length obtained in 30 DEG C of black dull cultivation 2 d.
The method of seedling stage assay Semen arachidis hypogaeae black rot resistant variety the most according to claim 2, it is characterised in that described Semen arachidis hypogaeae
The concentration of black rot hyphal suspension is 0.5%.
5. the method for seedling stage assay Semen arachidis hypogaeae black rot resistant variety described in any one of Claims 1-4 is studied at peanut breeding
Application in Resistance Identification.
Application the most according to claim 5, it is characterised in that be to utilize described seedling stage assay Semen arachidis hypogaeae black rot resistant variety
Method to peanut breeding material make batch screening in application.
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| CN109280715A (en) * | 2018-07-31 | 2019-01-29 | 仲恺农业工程学院 | A kind of LAMP primer group detecting peanut black rot and its rapid detection method and kit |
| CN112592956A (en) * | 2020-12-14 | 2021-04-02 | 浙江省农业科学院 | Method for identifying resistance to soybean anthracnose |
| CN114793676A (en) * | 2022-03-31 | 2022-07-29 | 江苏省农业科学院 | Method for inoculating seedling stage germs of cruciferous crops |
| CN115474487A (en) * | 2022-08-30 | 2022-12-16 | 仲恺农业工程学院 | Indoor ralstonia solanacearum inoculation method |
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| CN109280715B (en) * | 2018-07-31 | 2022-02-08 | 仲恺农业工程学院 | LAMP primer group for detecting peanut black rot, and rapid detection method and kit thereof |
| CN112592956A (en) * | 2020-12-14 | 2021-04-02 | 浙江省农业科学院 | Method for identifying resistance to soybean anthracnose |
| CN112592956B (en) * | 2020-12-14 | 2023-02-03 | 浙江省农业科学院 | Method for identifying resistance to soybean anthracnose |
| CN114793676A (en) * | 2022-03-31 | 2022-07-29 | 江苏省农业科学院 | Method for inoculating seedling stage germs of cruciferous crops |
| CN115474487A (en) * | 2022-08-30 | 2022-12-16 | 仲恺农业工程学院 | Indoor ralstonia solanacearum inoculation method |
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