CN106170301A - 一种模拟细胞体内环境的微流体器件及其应用 - Google Patents

一种模拟细胞体内环境的微流体器件及其应用 Download PDF

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Publication number
CN106170301A
CN106170301A CN201480001474.6A CN201480001474A CN106170301A CN 106170301 A CN106170301 A CN 106170301A CN 201480001474 A CN201480001474 A CN 201480001474A CN 106170301 A CN106170301 A CN 106170301A
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micro
microfluidic device
ramp structure
body cavity
miniflow
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蒋春盛
董毅
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Medvision Medical Systems Wuhan Co ltd
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Medvision Medical Systems Wuhan Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本发明公开了一种模拟细胞体内环境的微流体器件及其应用，包括进液口、出液口、以及至少一微流体腔；微流体腔具有入口通道与出口通道，入口通道连通进液口，出口通道连通出液口；微流体腔内设置众多微结构元素，其中具有微斜坡结构。该微流体器件能够模拟细胞体内环境，高效、柔和，利于细胞保护、识别和释放，增强并促进细胞和抗体的亲和作用，增加细胞和抗体的接触时间和面积，可用于癌症研究，各种癌症的临床诊断的研究，以及个人诊断肿瘤靶向治疗的研究。因其切变应力小，使细胞能够承受较高的流速，可以缩短样品处理时间，提高分离效率；从而实现了快速样品处理，减少生物标记的损伤，保护了生物标记的原始性和完整性。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201480001474.6A 2014-12-03 2014-12-03 一种模拟细胞体内环境的微流体器件及其应用 Pending CN106170301A (zh)

Applications Claiming Priority (1)

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PCT/CN2014/092950 WO2016086374A1 (zh) 2014-12-03 2014-12-03 一种模拟细胞体内环境的微流体器件及其应用

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CN106170301A true CN106170301A (zh) 2016-11-30

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WO (1) WO2016086374A1 (zh)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN106955384A (zh) * 2017-05-04 2017-07-18 中国药科大学 一种循环肿瘤细胞捕获装置

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110573882B (zh) * 2018-01-16 2021-06-25 株式会社爱蓓儿 血液凝固时间测量用卡匣以及血液凝固时间测量装置
CN113804658B (zh) * 2020-06-11 2023-06-06 京东方科技集团股份有限公司 微流控流道结构、检测系统及其使用方法

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US20020086436A1 (en) * 1992-05-21 2002-07-04 Biosite Incorporated Diagnostic devices and apparatus for the controlled movement of reagents without membranes
US20070080976A1 (en) * 2005-10-11 2007-04-12 Shaw-Hwa Parng Biochip with microchannels
US20130071611A1 (en) * 2011-09-21 2013-03-21 Stmicroelectronics S.R.L. Three-dimensional integrated structure having a high shape factor, and related forming method
CN103630470A (zh) * 2007-04-16 2014-03-12 通用医疗公司以马萨诸塞州通用医疗公司名义经营 使粒子在微通道中聚集的系统和方法
CN103998932A (zh) * 2011-06-29 2014-08-20 中央研究院 使用表面涂层对生物物质的捕获、纯化和释放

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US9180453B2 (en) * 2008-08-15 2015-11-10 University Of Washington Method and apparatus for the discretization and manipulation of sample volumes
US9957472B2 (en) * 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
CN103865752B (zh) * 2014-03-07 2015-10-28 复旦大学附属中山医院 循环肿瘤细胞捕获和分类磁性微流控芯片及其制造和使用

Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
US20020086436A1 (en) * 1992-05-21 2002-07-04 Biosite Incorporated Diagnostic devices and apparatus for the controlled movement of reagents without membranes
US20070080976A1 (en) * 2005-10-11 2007-04-12 Shaw-Hwa Parng Biochip with microchannels
CN103630470A (zh) * 2007-04-16 2014-03-12 通用医疗公司以马萨诸塞州通用医疗公司名义经营 使粒子在微通道中聚集的系统和方法
CN103998932A (zh) * 2011-06-29 2014-08-20 中央研究院 使用表面涂层对生物物质的捕获、纯化和释放
US20130071611A1 (en) * 2011-09-21 2013-03-21 Stmicroelectronics S.R.L. Three-dimensional integrated structure having a high shape factor, and related forming method

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* Cited by examiner, † Cited by third party
Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106955384A (zh) * 2017-05-04 2017-07-18 中国药科大学 一种循环肿瘤细胞捕获装置

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