CN106153899A - For detecting the device of lateral flow Multiple detection reagent - Google Patents
For detecting the device of lateral flow Multiple detection reagent Download PDFInfo
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Abstract
The invention discloses a kind of device for detecting lateral flow Multiple detection reagent, including light source, N number of optical fiber group, N number of first photoelectric conversion unit, detector unit and display lamp group, wherein first irradiates p-wire band corresponding on the detection light irradiation reagent that light source is produced by optical fiber；The fluorescence excited is sent to the first photoelectric conversion unit of correspondence by the first detection fiber；Fluorescence is converted to the signal of telecommunication and is supplied to detector unit by the first photoelectric conversion unit；Detector unit converts electrical signals to the digital code information shown for display lamp group, in order to user utilizes predetermined detection decision rule to determine testing result.The present invention realizes Multiple detection by the fluorescence signal of lateral flow Multiple detection reagent carries out detection with analysis, it is not required to moving fiber group and reagent during detection, by cooperating between detector unit and LED light, can effectively simplify detecting step, reduce detection complexity, shorten the detection time, save testing cost.
Description
Technical field
The present invention relates to field of biological medicine, particularly relate to a kind of device for detecting lateral flow Multiple detection reagent.
Background technology
Lateral flow Multiple detection technology has the features such as detection quick, easy, multiple is carried out simultaneously, it is widely used in Multiple detection field, as: multiple disease detects simultaneously, and single disease unlike signal thing detects simultaneously, virus Multi-genotype, Serotypes detection etc..
Viral disease includes the disease that acquired immune deficiency syndrome (AIDS), viral hepatitis, epidemic encephalitis type B etc. are caused by virus.As a example by viral hepatitis, viral hepatitis is serious, a long-term publilc health and hygienic issues in China, the polytype such as including A type, B-mode, the third type and penta type, wherein by hepatitis B virus (Hepatitis B Virus, it being called for short HBV) patient populations of hepatitis B that causes is most, and social danger is maximum.For a long time, the Clinics and Practices of hepatitis B, the detection method combined often by antigen, antibody, or by DNA (the Deoxyribonucleic Acid of hepatitis B virus, DNA (deoxyribonucleic acid)) detection realizes, and all of hepatitis B patient have employed the pattern of unified medication.The continuous propelling decoded along with HBV gene sequence, up to the present, is found that " A-H " eight kinds of hepatitis B gene types altogether, and China is based on " A-D " four kinds of genotype.Owing to the hepatitis B virus of different genotype has notable difference for toleration and the reaction of similar medicine, so, under unified medication pattern, it is difficult to guarantee the therapeutic effect of different hepatitis B patient completely.Therefore, in order to improve disease therapeuticing effect, the most all the more paid attention to by people with the novel method for the treatment of that personalized treatment, personalized medicine are Typical Representative.
Traditional detection and diagnostic method are owing to being limited to by self, it is impossible to provide the reference information being used for supporting the clinical diagnosis of personalized treatment or personalized medicine.Therefore, in order to realize hepatitis B individualized treatment, need a kind of detection method that hepatitis B virus can be carried out gene type, in order to for the hepatitis B patient of different genotype, the medicine using specific aim higher improves therapeutic effect.At present, the hepatitis B gene classifying method of document report is based primarily upon the principle that nucleic acid gene is analyzed, and i.e. first passes through the DNA sequence to hepatitis B virus and expands, and carries out detect to the DNA sequence after amplification the most again and analyzes and determine its genotype.This method is firstly the need of relying on PCR (Polymerase Chain Reaction, polymerase chain reaction) amplified reaction obtains abundant hepatitis B virus DNA fragment, the methods such as hybridization, fluorescent labeling are used to detect the most again, it is disadvantageous in that: detection equipment is many, time length, operation complexity, cost are high, it is difficult to promote.Therefore, in order to benefit hepatitis B and other viral diseases patient, improve therapeutic effect, it is achieved the personalized diagnosis and treatment of clinical acceptable viral disease, need to research and propose simpler, convenient, reliable, accurate, the gene typing detection device of low cost.
Summary of the invention
The invention provides a kind of device for detecting lateral flow Multiple detection reagent, by the fluorescence signal of lateral flow Multiple detection reagent is detected and analyzes, realize Multiple detection, such as HBV, HIV (Human Immunodeficiency Virus, HIV (human immunodeficiency virus)) etc. gene typing detection, and the joint-detection etc. of multiple disease.During detection, it is not required to the lateral flow Multiple detection reagent of moving fiber group and such as reagent paper, and detects signal and processed by detector unit, and shown testing result by display lamp, therefore, it is possible to effectively simplify detecting step, reduce detection complexity, shorten the detection time, save testing cost.
According to the present invention, provide a kind of device for detecting lateral flow Multiple detection reagent, including light source, N number of optical fiber group, N number of first photoelectric conversion unit, detector unit and display lamp group, each optical fiber group includes the first irradiation optical fiber and the first detection fiber respectively, wherein in i-th optical fiber group, first irradiation optical fiber is corresponding with i-th p-wire band on lateral flow Multiple detection reagent, first detection fiber is corresponding with i-th the first photoelectric conversion unit, 1≤i≤N, N is the number of p-wire band on lateral flow Multiple detection reagent, wherein:
Light source, is used for producing detection light；
First irradiates optical fiber, irradiates p-wire band corresponding on lateral flow Multiple detection reagent for the detection light produced by light source；
First detection fiber, the fluorescence excited after p-wire band detected light corresponding on lateral flow Multiple detection reagent being irradiated is sent to the first photoelectric conversion unit of correspondence；
First photoelectric conversion unit, for the fluorescence received is converted to the signal of telecommunication, and is supplied to detector unit by the signal of telecommunication of generation；
Detector unit, for based on detecting decision rule accordingly, is converted to corresponding digital code information by the N number of signal of telecommunication received；
Display lamp group, encodes information for display digit, in order to user utilizes predetermined detection decision rule to determine testing result.
In one embodiment, also include N number of second photoelectric conversion unit and N number of subtrator, each optical fiber group respectively further comprises the second irradiation optical fiber and the second detection fiber, wherein in i-th optical fiber group, second irradiation optical fiber is corresponding with the background area of i-th p-wire band on lateral flow Multiple detection reagent, second detection fiber is corresponding with i-th the second photoelectric conversion unit, and i-th the first photoelectric conversion unit is corresponding with i-th subtrator, wherein respectively with i-th the second photoelectric conversion unit:
Second irradiates optical fiber, irradiates p-wire band background area corresponding on lateral flow Multiple detection reagent for the detection light produced by light source；
Second detection fiber, the fluorescence excited after p-wire band background area detected light corresponding on lateral flow Multiple detection reagent being irradiated is sent to the second photoelectric conversion unit of correspondence；
Second photoelectric conversion unit, for being converted to the signal of telecommunication by the fluorescence received；
Subtrator, for the signal of telecommunication provided by corresponding first photoelectric conversion unit, the signal of telecommunication provided with corresponding second photoelectric conversion unit subtracts each other, and to obtain removing the signal of telecommunication of ambient interferences, the signal of telecommunication of the removal ambient interferences obtained is supplied to detector unit.
In one embodiment, each digitally encoded signal in digital code information and the display lamp one_to_one corresponding in display lamp group.
In one embodiment, decoding unit is also included, wherein:
Decoding unit, for by detector unit provide digital code information be decoded, in order to digital code information is simplified, and will simplify after digital code information be supplied to display lamp group；
Display lamp group is particularly shown the digital code information received, in order in display lamp group, only one display lamp is lit.
In one embodiment, display lamp is lighted when corresponding digital code information is 1, extinguishes when corresponding digital code information is 0.
In one embodiment, first irradiates the distance optical port and lateral flow Multiple detection reagent of optical fiber and the second irradiation optical fiber less than 2mm.
In one embodiment, first irradiates optical fiber and the second optical port that goes out irradiating optical fiber is shaped as point-like or strip, in order to the detection light that light source produces first irradiate that optical fiber and second irradiates optical fiber go out to be formed at optical port point shaped laser spot or line spot.
In one embodiment, the light inputting end mouth of the first detection fiber and the second detection fiber and the distance of lateral flow Multiple detection reagent are less than 2mm.
In one embodiment, the distance going out optical port and corresponding photoelectric conversion unit photosensitive surface of the first detection fiber and the second detection fiber is less than 0.5mm.
In one embodiment, the wavelength of detection light is 360nm-410nm.
The device for detecting lateral flow Multiple detection reagent of the present invention, by the fluorescence signal of lateral flow Multiple detection reagent is detected and analyzes, realize Multiple detection, such as gene typing detections such as HBV, HIV, during detection, be not required to moving fiber group and lateral flow Multiple detection reagent, and detect signal and processed by detector unit, and shown testing result by display lamp, therefore, it is possible to effectively simplify detecting step, reduce detection complexity, reduce the detection time, save testing cost.
Accompanying drawing explanation
For the technical scheme being illustrated more clearly that in the embodiment of the present invention, the accompanying drawing used required in embodiment or description will be briefly described below, apparently, accompanying drawing in describing below is only some embodiments of the present invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is that the present invention is for detecting the schematic diagram of one embodiment of device of lateral flow Multiple detection reagent.
Fig. 2 is that the present invention is for detecting device illumination path and the schematic diagram of detection one embodiment of light path of lateral flow Multiple detection reagent.
Fig. 3 is that the present invention is for detecting the operation principle schematic diagram of the device of lateral flow Multiple detection reagent.
Fig. 4 is the operation principle schematic diagram that the present invention is applied to an embodiment of HBV typing for the device detecting lateral flow Multiple detection reagent.
Fig. 5 is that the present invention is applied to the schematic diagram of detector unit in an embodiment of HBV typing for the device detecting lateral flow Multiple detection reagent.
Fig. 6 is that the present invention is applied to the schematic diagram of gene type synopsis in an embodiment of HBV typing for the device detecting lateral flow Multiple detection reagent.
Fig. 7 is that the present invention is applied to the schematic diagram of gene type synopsis in another embodiment of HBV typing for the device detecting lateral flow Multiple detection reagent.
Fig. 8 is the operation principle schematic diagram that the present invention is applied to an embodiment of HIV typing for the device detecting lateral flow Multiple detection reagent.
Fig. 9 is that the present invention is applied to the schematic diagram of detector unit in an embodiment of HIV typing for the device detecting lateral flow Multiple detection reagent.
Figure 10 is the schematic diagram that the present invention is applied to the middle gene type synopsis of an embodiment of HIV typing for the device detecting lateral flow Multiple detection reagent.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Description only actually at least one exemplary embodiment is illustrative below, never as to the present invention and application thereof or any restriction of use.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly fall into the scope of protection of the invention.
Unless specifically stated otherwise, the parts illustrated the most in these embodiments and positioned opposite, the numerical expression of step and numerical value do not limit the scope of the invention.
Simultaneously, it should be appreciated that for the ease of describing, the size of the various piece shown in accompanying drawing is not to draw according to actual proportionate relationship.
May be not discussed in detail for technology, method and apparatus known to person of ordinary skill in the relevant, but in the appropriate case, described technology, method and apparatus should be considered to authorize a part for description.
In shown here and all examples of discussing, any occurrence should be construed as merely exemplary rather than as limiting.Therefore, other example of exemplary embodiment can have different values.
It should also be noted that similar label and letter represent similar terms in following accompanying drawing, therefore, the most a certain Xiang Yi accompanying drawing is defined, then need not it is further discussed in accompanying drawing subsequently.
Fig. 1 is that the present invention is for detecting the schematic diagram of one embodiment of device of lateral flow Multiple detection reagent.As it is shown in figure 1, fluorescence detection device includes light source 1, N number of optical fiber group 2i, N number of first photoelectric conversion unit 31i, detector unit 4 and display lamp group 5, wherein, 1≤i≤N, N are the number of p-wire band on lateral flow Multiple detection reagent 8.Each optical fiber group 2i includes the first irradiation optical fiber 21i and the first detection fiber 22i respectively, in optical fiber group 2i, first irradiation optical fiber 21i is corresponding with i-th p-wire band on lateral flow Multiple detection reagent 8, and the first detection fiber 22i is corresponding, wherein with the first photoelectric conversion unit 31i:
Light source 1 is used for producing detection light.
In i-th optical fiber group, first irradiates optical fiber 21i irradiates i-th p-wire band corresponding on lateral flow Multiple detection reagent 8 for the detection light produced by light source 1.The fluorescence that first detection fiber 22i excites after i-th p-wire band detected light corresponding on lateral flow Multiple detection reagent 8 being irradiated is sent to the first photoelectric conversion unit 31i of correspondence.
First photoelectric conversion unit 31i for being converted to the signal of telecommunication by the fluorescence received, and the signal of telecommunication of generation is supplied to detector unit 4.
Detector unit 4 is for based on detection decision rule, being converted to corresponding digital code information by the N number of signal of telecommunication received.
Display lamp group 5 encodes information for display digit, in order to user utilizes predetermined detection decision rule to determine testing result.
The device for detecting lateral flow Multiple detection reagent based on the present invention, the detection light that light source 1 produces irradiates optical fiber 21i through first and is radiated on i-th test strip of lateral flow Multiple detection reagent 8 correspondence, the fluorescence inspired is sent to the first photoelectric conversion unit 31i through the first detection fiber 22i, fluorescence is converted to the signal of telecommunication and is supplied to detector unit 4 by the first photoelectric conversion unit 31i, detector unit 4 is based on detecting decision rule accordingly, the N number of signal of telecommunication received is converted to corresponding digital code information, display lamp group 5 encodes information for display digit, so that user utilizes predetermined detection decision rule to determine testing result, realize HBV, the typing detection of the viral genes such as HIV.Need not realize p-wire Scan by moving fiber group 2i and lateral flow Multiple detection reagent 8 during detection, therefore, it is possible to effectively simplify detecting step, reduce detection complexity, shorten the detection time, save testing cost.Compared with classifying method based on nucleic acid gene sequence, the present invention is more beneficial for realizing gene typing convenient, quick, efficient detection.As example, lateral flow Multiple detection reagent can be reagent paper, but be not limited thereto.
Fig. 2 is that the present invention is for detecting device illumination path and the schematic diagram of detection one embodiment of light path of lateral flow Multiple detection reagent.As shown in Figure 2, also include N number of second photoelectric conversion unit 32i, the second irradiation optical fiber 23i and the second detection fiber 24i is respectively further comprised in i-th optical fiber group, wherein the second irradiation optical fiber 23i is corresponding with the background area of i-th p-wire band on lateral flow Multiple detection reagent, second detection fiber 24i is corresponding, wherein with the second photoelectric conversion unit 32i:
Second irradiates optical fiber 23i irradiates i-th p-wire band background area corresponding on lateral flow Multiple detection reagent 8 for the detection light produced by light source 1.
The fluorescence that second detection fiber 24i excites after i-th p-wire band background area detected light corresponding on lateral flow Multiple detection reagent 8 being irradiated is sent to the second photoelectric conversion unit 32i of correspondence.
Second photoelectric conversion unit 32i is for being converted to the signal of telecommunication by the fluorescence received.
Fig. 3 is that the present invention is for detecting the operation principle schematic diagram of the device of lateral flow Multiple detection reagent.As it is shown on figure 3, also include N number of subtrator 6i.Subtrator 6i is for the signal of telecommunication provided by corresponding first photoelectric conversion unit 31i, the signal of telecommunication provided with corresponding second photoelectric conversion unit 32i subtracts each other, to obtain removing the signal of telecommunication of ambient interferences, the signal of telecommunication of the removal ambient interferences obtained is supplied to detector unit 4.
During detection, in order to eliminate systematic error, take the i-th p-wire band near zone background area as i-th p-wire band of lateral flow Multiple detection reagent 8, the electrical information that corresponding first photovoltaic element 31i and the second photovoltaic element 32i provides is subtracted each other, effectively to remove ambient interferences by subtrator 6i.
Preferably, each digitally encoded signal in digital code information and the display lamp one_to_one corresponding in display lamp group 5.It is explained below by specific embodiment.
Preferably, between detector unit 4 and display lamp group 5, also include that decoding unit (not shown) is for being decoded the digital code information that detector unit 4 provides, so that digital code information is simplified, and will simplify after digital code information be supplied to display lamp group 5.Display lamp group 5 is particularly shown the digital code information received, in order in display lamp group, only one display lamp is lit.It is explained below by specific embodiment.
Preferably, the display lamp in display lamp group 5 is lighted when corresponding digital code information is 1, extinguishes when corresponding digital code information is 0.
Preferably, first irradiates the distance optical port and lateral flow Multiple detection reagent 8 of optical fiber 21i and second irradiation optical fiber 23i less than 2mm.
Preferably, the first irradiation optical fiber 21i and the second optical port that goes out irradiating optical fiber 23i are shaped as point-like or strip, in order to the detection light that light source 1 produces goes out to be formed at optical port point shaped laser spot or line spot the first irradiation optical fiber 21i's and the second irradiation optical fiber 23i.
Preferably, the light inputting end mouth of the first detection fiber 22i and the second detection fiber 24i and the distance of lateral flow Multiple detection reagent 8 are less than 2mm.
Preferably, the optical port that goes out of the first detection fiber 22i and the second detection fiber 24i is less than 0.5mm with the distance of the photosensitive surface of corresponding first photoelectric conversion unit 31i and the second photoelectric conversion unit 32i, and allows to overlap.
Preferably, the wavelength of the detection light that light source 1 sends is 360nm-410nm.Such as light source 1 is LED (Light Emitting Diode, Light-Emitting Diode), it is provided that a length of 360nm-410nm of detection light wave, p-wire band produce the HONGGUANG that fluorescence is wavelength 615nm.
Fig. 4 is present invention operation principle schematic diagram of detector unit when the device detecting lateral flow Multiple detection reagent is applied to HBV typing.Fig. 5 is present invention schematic diagram of detector unit 4 when the device detecting lateral flow Multiple detection reagent is applied to HBV typing.As shown in Figure 4, when the present invention is applied to HBV typing, 6 p-wire bands are had on lateral flow Multiple detection reagent 8, i.e. N=6, being respectively C control line, T5 detection line, T4 detection line, T3 detection line, T2 detection line and T1 and detect line, each test features band signal in Fig. 4 is the signal after removing ambient interferences.In Fig. 5, detector unit 4 includes I-V amplifier, and the current signal of reception is converted to voltage signal.Combined by comparator and analog switch, select relatively strong test signal.The implication that each p-wire band is corresponding is as follows:
C control line is used for detecting Quality Control.When sample normally completes chromatography reaction, the fluorescence signal of C control line should be more than the first threshold value, and otherwise, owing to the reasons such as paper slip inefficacy cause sample to be not properly completed chromatography reaction, detection makes mistakes.In the present embodiment, the first threshold value is 200mV, and those skilled in the art are it will be appreciated that for different detection environment, the first threshold value can be other value.
T5 p-wire is used for HBV virus antigen detection.In the present embodiment when sample normally completes chromatography reaction, when the fluorescence signal of T5 p-wire is more than the first threshold value, represents in sample and there is HBV virus antigen, i.e. HBV test positive, otherwise, HBV is detected as feminine gender.
T4 p-wire for HBV gene type A virus protein detection, and with genotype B, C, D virus protein without specific reaction.In the present embodiment when sample normally completes chromatography reaction, the fluorescence signal of T4 p-wire is more than the second threshold value, and compared with T3-T1 p-wire, during its amplitude maximum, testing result is genotype A.In the present embodiment, the second threshold value is 100mV, and those skilled in the art are it will be appreciated that for different detection environment, the second threshold value can be other value.
T3 p-wire for HBV gene type B virus protein detection, and with genotype A, C, D virus protein without specific reaction.When sample normally completes chromatography reaction, the fluorescence signal of T3 p-wire is more than the second threshold value, and compared with T4, T2, T1 p-wire, during its amplitude maximum, testing result is genotype B.
T2 p-wire for HBV gene type C, D virus protein detection, and with genotype A, B virus protein without specific reaction.When sample normally completes chromatography reaction, the fluorescence signal of T2 p-wire is more than the second threshold value, and compared with T4, T3, T1 p-wire, during its amplitude maximum, again by comparing the T1 p-wire fluorescence signal of T2 p-wire fluorescence signal and 4 times, compare fluorescence signal and second threshold value of T1 p-wire simultaneously, if the T1 signal that T2 signal is more than 4 times, be then c-type；If being Error when the T1 signal of T2 signal no more than 4 times and T1 signal no more than the second threshold value；If being D type when the T1 signal of T2 signal no more than 4 times and T1 signal are more than the second threshold value.
T1 p-wire is for HBV gene type A, the virus protein detection of D, and wherein, the detection sensitivity of genotype D is significantly higher than genotype A, and with genotype B, C virus protein without specific reaction.When sample normally completes chromatography reaction, the fluorescence signal of T1 p-wire is more than the second threshold value, and compared with T4, T3, T2 p-wire, during its amplitude maximum, testing result is genotype D.
In the present embodiment, detector unit 4 includes the unit such as the reference voltage unit of I-V amplifier, comparator, analog switch, base amplifier, offer threshold voltage, the signal of telecommunication received is converted to corresponding digital code information, in order to display lamp group 5 shows according to digital code information.Display lamp group 5 includes 8 display lamps.As shown in Figure 6, wherein " 1 " represents that display lamp is bright to HBV gene typing synopsis based on detection decision rule, and " 0 " represents that display lamp goes out, and " X " represents don't care state.As shown in Fig. 4 and Fig. 6, testing result includes: A type, Type B, c-type, D type, Error (makeing mistakes), cannot typing/UN and feminine gender/N.User only needs the display according to display lamp group 5, need not moving fiber group 2i and lateral flow Multiple detection reagent 8 when detection, and comparison HBV typing synopsis shown in Fig. 6 i.e. can get HBV typing testing result, reduces detection difficulty, simplify detection program.Meanwhile, as it is shown in figure 5, detector unit 4 carries out parallel processing to detection signal, improve detection rates.Wherein, as shown in Figure 5, reference 51 is to remove the C control line current signal of background, reference 52 is to remove the T5 p-wire current signal of background, and reference 53 is to remove the T4 p-wire current signal of background, and reference 54 is to remove the T3 p-wire current signal of background, reference 55 is to remove the T2 p-wire current signal of background, reference 56 is to remove the T1 p-wire current signal of background, and reference 57 is 200mV reference voltage, and reference 58 is 100mV reference voltage.
Preferably, in one embodiment, between detector unit 4 and display lamp group 5, also include decoding unit, for the digital code information that detector unit 4 provides being decoded, so that digital code information is simplified, and will simplify after digital code information be supplied to display lamp group 5.Display lamp group 5 is particularly shown the digital code information received, in order in display lamp group, only one display lamp is lit.HBV typing synopsis after simplifying in Fig. 7 the present embodiment, by decoding unit, digital code information is simplified, make user only need to can draw HBV typing testing result according to the HBV typing synopsis shown in Fig. 7 according to the light on and off of a display lamp, further simplify the operating procedure of testing staff.
Fig. 8 is the operation principle schematic diagram that the present invention is applied to an embodiment of HIV typing for the device detecting lateral flow Multiple detection reagent.As shown in Figure 8, when the present invention is applied to HIV typing, including 3 p-wire bands, i.e. N=3, it is respectively as follows: C control line, T2 p-wire, T1 p-wire, wherein:
C control line is used for detecting Quality Control.When sample normally completes chromatography reaction, the fluorescence signal of C control line should be more than the 3rd threshold value, and otherwise, owing to the reasons such as paper slip inefficacy cause sample to be not properly completed chromatography reaction, detection makes mistakes.In the present embodiment, the 3rd threshold value is 200mV, and those skilled in the art are it will be appreciated that for different detection environment, the 3rd threshold value can be other value.
T2 p-wire is used for HIV-1 antibody test, and with HIV-2 type antibody without specific reaction.When sample normally completes chromatography reaction, when the fluorescence signal of T2 p-wire is more than four threshold values, represents in sample and there is HIV-1 antibody, be HIV-1 type, otherwise, for non-HIV-1 type.In the present embodiment, the 4th threshold value is 100mV, and those skilled in the art are it will be appreciated that for different detection environment, the 4th threshold value can be other value.
T1 p-wire is used for HIV-2 antibody test, and with HIV-1 type antibody without specific reaction.When sample normally completes chromatography reaction, when the fluorescence signal of T1 p-wire is more than four threshold values, represents in sample and there is HIV-2 antibody, be HIV-2 type, otherwise, for non-HIV-2 type.
Fig. 9 is present invention schematic diagram of detector unit 4 when the device detecting lateral flow Multiple detection reagent is applied to HIV typing.In the present embodiment, detector unit 4 includes the unit such as the reference voltage unit of I-V amplifier, comparator, analog switch, base amplifier, offer threshold voltage, the signal of telecommunication received is converted to corresponding digital code information, in order to display lamp group 5 shows according to digital code information.Display lamp group 5 includes 4 display lamps.HIV gene type synopsis is as shown in Figure 10.As shown in Fig. 8 and Figure 10, testing result includes: HIV-1 type, HIV-2 type, Error (makeing mistakes) and feminine gender/N.User only needs the display according to display lamp group 5, need not moving fiber group 2i and lateral flow Multiple detection reagent 8 when detection, and comparison HIV typing synopsis shown in Figure 10 i.e. can get HIV typing testing result, reduces detection difficulty, simplify detection program.Wherein, in fig .9, reference 91 is to remove the C control line current signal of background, reference 92 is to remove the T2 p-wire current signal of background, reference 93 is to remove the T1 p-wire current signal of background, and reference 94 is 200mV reference voltage, and reference 95 is 100mV reference voltage.
One of ordinary skill in the art will appreciate that all or part of step realizing above-described embodiment can be completed by hardware, relevant hardware can also be instructed by program to complete, described program can be stored in a kind of computer-readable recording medium, storage medium mentioned above can be read only memory, disk or CD etc..
Description of the invention is given for example with for the sake of describing, and is not exhaustively or limit the invention to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Selecting and describing embodiment is in order to the principle of the present invention and actual application are more preferably described, and makes those of ordinary skill in the art it will be appreciated that the present invention thus design are suitable to the various embodiments with various amendments of special-purpose.
Claims (10)
1. the device being used for detecting lateral flow Multiple detection reagent, it is characterised in that bag
Include light source, N number of optical fiber group, N number of first photoelectric conversion unit, detector unit and display lamp group,
Each optical fiber group includes the first irradiation optical fiber and the first detection fiber respectively, wherein at i-th light
In fine group, first irradiates optical fiber and i-th p-wire band pair on lateral flow Multiple detection reagent
Should, the first detection fiber is corresponding with i-th the first photoelectric conversion unit, and 1≤i≤N, N are side
The number of p-wire band on stream Multiple detection reagent, wherein:
Light source, is used for producing detection light；
First irradiates optical fiber, and the detection light for being produced by light source irradiates the examination of lateral flow Multiple detection
P-wire band corresponding in agent；
First detection fiber, for by p-wire band corresponding on lateral flow Multiple detection reagent
The fluorescence that detected light excites after irradiating is sent to the first photoelectric conversion unit of correspondence；
First photoelectric conversion unit, for the fluorescence received being converted to the signal of telecommunication, and will be raw
The signal of telecommunication become is supplied to detector unit；
Detector unit, for based on detection decision rule, turning the N number of signal of telecommunication received
It is changed to corresponding digital code information；
Display lamp group, encodes information for display digit, in order to user utilizes predetermined detection to sentence
Set pattern then determines testing result.
Device the most according to claim 1, it is characterised in that also include N number of second
Photoelectric conversion unit and N number of subtrator, each optical fiber group respectively further comprises the second irradiation light
Fibre and the second detection fiber, wherein in i-th optical fiber group, second irradiates optical fiber and lateral flow
On Multiple detection reagent, the background area of i-th p-wire band is corresponding, the second detection fiber with
I-th the second photoelectric conversion unit is corresponding, i-th the first photoelectric conversion unit and i-th the
Two photoelectric conversion units are corresponding with i-th subtrator, wherein respectively:
Second irradiates optical fiber, and the detection light for being produced by light source irradiates the examination of lateral flow Multiple detection
P-wire band background area corresponding in agent；
Second detection fiber, for by p-wire band corresponding on lateral flow Multiple detection reagent
The fluorescence that background area detected light excites after irradiating is sent to the second opto-electronic conversion list of correspondence
Unit；
Second photoelectric conversion unit, for being converted to the signal of telecommunication by the fluorescence received；
Subtrator, for the signal of telecommunication provided by corresponding first photoelectric conversion unit, with corresponding
The signal of telecommunication that second photoelectric conversion unit provides subtracts each other, to obtain removing the telecommunications of ambient interferences
Number, the signal of telecommunication of the removal ambient interferences obtained is supplied to detector unit.
Device the most according to claim 1 and 2, it is characterised in that
Each digitally encoded signal in digital code information and the display lamp one in display lamp group
One is corresponding.
Device the most according to claim 3, it is characterised in that also include decoding unit,
Wherein:
Decoding unit, for being decoded the digital code information that detector unit provides, in order to
Digital code information is simplified, and will simplify after digital code information be supplied to display lamp
Group；
Display lamp group is particularly shown the digital code information received, in order in display lamp group only one
Individual display lamp is lit.
Device the most according to claim 4, it is characterised in that
Display lamp is lighted when corresponding digital code information is 1, believes at corresponding digital coding
Extinguish when breath is 0.
Device the most according to claim 4, it is characterised in that
First optical port that goes out irradiating optical fiber and the second irradiation optical fiber tries with lateral flow Multiple detection
The distance of agent is less than 2mm.
Device the most according to claim 6, it is characterised in that
First optical port that goes out irradiating optical fiber and the second irradiation optical fiber is shaped as point-like or strip, with
Just the detection light that light source produces irradiates optical fiber and second first and irradiates the going out at optical port of optical fiber
Form corresponding some shaped laser spot or line spot.
Device the most according to claim 4, it is characterised in that
The light inputting end mouth of the first detection fiber and the second detection fiber tries with lateral flow Multiple detection
The distance of agent is less than 2mm.
Device the most according to claim 8, it is characterised in that
First detection fiber and the second detection fiber go out optical port and corresponding photoelectric conversion unit
The distance of photosensitive surface is less than 0.5mm.
Device the most according to claim 3, it is characterised in that
The wavelength of detection light is 360nm-410nm.
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| CN201510128087.5A CN106153899B (en) | 2015-03-24 | 2015-03-24 | Device for detecting lateral flow Multiple detection reagent |
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| CN201510128087.5A CN106153899B (en) | 2015-03-24 | 2015-03-24 | Device for detecting lateral flow Multiple detection reagent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201535749U (en) * | 2008-07-14 | 2010-07-28 | 马义才 | Quantum dot mark test strip quantitative detection system based on CMOS picture sensor |
| CN103063834A (en) * | 2012-12-28 | 2013-04-24 | 三诺生物传感股份有限公司 | Method and system for analysis of immune quantitative chromatographic assay strip |
| CN203688558U (en) * | 2013-08-27 | 2014-07-02 | 山东诺安诺泰信息系统有限公司 | Dry-type portable hematuria biochemical detector |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201535749U (en) * | 2008-07-14 | 2010-07-28 | 马义才 | Quantum dot mark test strip quantitative detection system based on CMOS picture sensor |
| CN103063834A (en) * | 2012-12-28 | 2013-04-24 | 三诺生物传感股份有限公司 | Method and system for analysis of immune quantitative chromatographic assay strip |
| CN203688558U (en) * | 2013-08-27 | 2014-07-02 | 山东诺安诺泰信息系统有限公司 | Dry-type portable hematuria biochemical detector |
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