CN106148118A - A kind of cordyceps militaris liquor preparation method - Google Patents

A kind of cordyceps militaris liquor preparation method Download PDF

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Publication number
CN106148118A
CN106148118A CN201510185793.3A CN201510185793A CN106148118A CN 106148118 A CN106148118 A CN 106148118A CN 201510185793 A CN201510185793 A CN 201510185793A CN 106148118 A CN106148118 A CN 106148118A
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China
Prior art keywords
agar
cordyceps
liquor
cordyceps militaris
container
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Pending
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CN201510185793.3A
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Chinese (zh)
Inventor
樊美珍
纪伟
陈奇超
孙长胜
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Zhejiang Faya Biological Pharmaceutical Co Ltd
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Zhejiang Faya Biological Pharmaceutical Co Ltd
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Priority to CN201510185793.3A priority Critical patent/CN106148118A/en
Publication of CN106148118A publication Critical patent/CN106148118A/en
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Abstract

The invention discloses a kind of cordyceps militaris liquor preparation method, it is characterised in that the method comprises the steps: step a, in wine holding container, inject agar;Step b, is inserted perpendicularly into Cordyceps in agar;Step c, injects agar again in container;Step d, injects Chinese liquor in container, seals;Step e, stores more than 45 days;Wherein, the concentration of agar described in step c is higher than the concentration of agar described in step a, and concentration difference is 0.5~1%.Cordyceps militaris liquor good looking appearance of the present invention, and active constituent content is high, its adenosine content is 15.33 times of Cordyceps powder, and polyoses content is 6.05 times of Cordyceps powder.

Description

A kind of cordyceps militaris liquor preparation method
Technical field
The present invention relates to a kind of cordyceps militaris liquor preparation method, be specifically related to one agar culture medium and fix Cordyceps bubble The method of wine.
Background technology
Cordyceps militaris (L.) Link., Periostracum cicadae are the Cordyceps kinds of common artificial culture.
Cordyceps militaris (L.) Link. (Cordyceps) is Ascomycotina, Ergota Zoopagales, Clavicipitaceae, the type sepecies of Cordyceps, Formal name used at school is Cordyceps militaris.The traditional Chinese medical science is thought, Cordyceps enters lung kidney two warp, can tonifying the lung cloudy, again can Kidney-replenishing, cures mainly and suffers from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, and weak after being ill, chronic cough is weak, phthisical cough expectorant blood, Spontaneous sweatings etc., are a kind of Chinese medicine that can balance simultaneously, regulate negative and positive.
Periostracum cicadae (Cordyceps cicadae Shing) is the complex after Clavicipitaceae fungus Paecilomyces cicadidae(Miquel)Samson parasitism bamboo Cicadae nymph, be with The Cordyceps that Cordyceps is similar.Have raising immunity, resisting fatigue, guarantor's kidney, improve sleep, antitumor, Protect the liver, the multiple action such as radioprotective and improving eyesight, be magical ancient Chinese medicine.The Periostracum cicadae of artificial culture contains manna The chemical compositions such as alcohol, polysaccharide, aminoacid, cordycepin, adenosine, Periostracum cicadae can alternative as Cordyceps Product, equally reach the effect of nourishing and health preserving.
Being steep in wine by Cordyceps is the usual alimentation method used, and its brew process is: (can also by Cordyceps Together with other drug brewed, such as Radix Ginseng, Fructus Lycii etc.) placed in the vessel and pour Chinese liquor into, seal, put Put suitable natural law the most drinkable.Using this brewing method, Cordyceps is at random in a reservoir, and have Swim in wine list layer, it has not been convenient to outwards pouring;The form of wine is bad simultaneously, does not highlight Cordyceps vision U.S. Impression.
Summary of the invention
For problems of the prior art, the invention provides a kind of cordyceps militaris liquor preparation method, the method Agar culture medium is set at container bottom, utilizes agar culture medium to fix Cordyceps, Chinese liquor is poured in container, Cordyceps will not be at random and floating, facilitates outside pouring, embodies Cordyceps vision in wine beautiful simultaneously.
It is an object of the invention to be achieved through the following technical solutions:
A kind of cordyceps militaris liquor preparation method, the method comprises the steps:
Step a, injects agar in wine holding container;
Step b, is inserted perpendicularly into Cordyceps in agar;
Step c, injects agar again in container;
Step d, injects Chinese liquor in container, seals;
Step e, stores more than 45 days;
Wherein, the concentration of agar described in step c is higher than the concentration of agar described in step a, and concentration difference is 0.5~1%.
Further, agar height described in step a is 0.5~2cm.
Further, Cordyceps described in step b is Cordyceps militaris (L.) Link. or Periostracum cicadae.
Further, agar height described in step c is 0.5~1cm.
Further, step d inject Chinese liquor to exceeding Cordyceps 1~2cm at, standing 30~60min, again Inject Chinese liquor at bottleneck 1~2cm.
Further, the step filtered also is included before cordyceps militaris liquor is drunk.
Further, the preparation method of described agar is: is dissolved in water by agar and boils 30min, uses subpackage Device loads in triangular flask, is made into the agar solution of desired concn, and loading amount is 1/3, tampon beyond the Great Wall;Put sterilizing In Guo, sterilizing 0.5h under 0.11Mpa pressure, fall pound naturally;Take out natural cooling while hot, treat temperature It is down to about 40 DEG C, the funnel of agar sterilizing is joined in wine holding container.Melt after agar heating, Solid is become after being housed in container cooling.
Agar used in the present invention is food stage agar.The Cordyceps used is amplification culture according to a conventional method Cordyceps.
The present invention also provides for a kind of cordyceps militaris liquor, and described cordyceps militaris liquor is prepared as follows:
Step a, injects agar in wine holding container;
Step b, is inserted perpendicularly into Cordyceps in agar;
Step c, injects agar again in container;
Step d, injects Chinese liquor in container;
Step e, stores more than 45 days;
Wherein, the concentration of agar described in step c is higher than the concentration of agar described in step a, and concentration difference is 0.5~1%.
Further, agar height described in step a is 0.5~2cm.
Further, Cordyceps described in step b is Cordyceps militaris (L.) Link. or Periostracum cicadae.
Further, agar height described in step c is 0.5~1cm.
Further, step d inject Chinese liquor to exceeding Cordyceps 1~2cm at, standing 30~60min, again Inject Chinese liquor at bottleneck 1~2cm.
Further, the step filtered also is included before cordyceps militaris liquor is drunk.
Cordyceps militaris liquor prepared by the inventive method has the advantage that
1. utilizing agar to fix Cordyceps militaris (L.) Link. or Periostracum cicadae, pour in container by Chinese liquor when steeping in wine, Cordyceps will not be at random With floating, facilitate outside pouring;2. Cordyceps major part in wine in vertical state, too increases this device Aesthetic, appear to be the Cordyceps in bottle, thus in drinking, give the visual experience of U.S.;③ Being more beneficial for the dissolution of Cordyceps effective ingredient, its adenosine content of cordyceps militaris liquor of the present invention is 15.33 times of Cordyceps powder, Polyoses content is 6.05 times of Cordyceps powder, it is seen then that cordyceps militaris liquor is the most undue for Cordyceps sporophore Content is higher, better.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of cordyceps militaris liquor container of the present invention.
In figure: 1, bottle;2, bottle cap;3, agar culture medium;4, Cordyceps;5, wine.
Effect experimental examples
Cordyceps militaris liquor preparation method comprises the steps:
Step a, injects the agar of 1.5% in wine holding container, and height is 1cm;
Step b, is inserted perpendicularly into Periostracum cicadae/Cordyceps militaris (L.) Link. in agar;
Step c, scheme one: be not injected into agar;
Scheme two: injecting the agar of 1.5%, height is 0.5cm;
Scheme three: injecting the agar of 2%, height is 0.5cm;
Step d, in container, injection Chinese liquor is to exceeding at Cordyceps 1~2cm, standing 30~60min, then Secondary injection Chinese liquor, at bottleneck 1~2cm, seals;
Step e, stores more than 45 days, filters, obtains cordyceps militaris liquor.
Investigating cordyceps militaris liquor outward appearance and component content prepared by different schemes, result see table.
Above-mentioned test result indicate that, after inserting Cordyceps, add the agar of higher concentration, be conducive to Cordyceps Fixing, be that Cordyceps keeps plumbness, without at random and floating, good looking appearance;Additionally, scheme three Cordyceps The most orderly is fixed on agar, beneficially the dissolution of effective ingredient, and cordyceps militaris liquor active constituent content is higher than The cordyceps militaris liquor of scheme one, two preparation.
Detailed description of the invention
Embodiment 1 Cordyceps cultural method
The preparation of 1 culture medium
Inclined-plane and seed liquor culture medium: Rhizoma Solani tuber osi liquor 20%, white sugar 3.5%, yeast leaching powder 0.5%.
Solid medium: by white sugar 3.5%, yeast extract powder 0.5%, VB11 0mg/1000ml Ratio be made into nutritional solution, rice and nutritional solution are formed by the proportions of 1:1.6.
2 condition of culture
2.1 mycelia culture management
Send out bacterium room temperature and be preferably 18~23 DEG C, relative air humidity 65%~85%.Shading;Indoor cultivation 6~8d, cover with mycelia.
2.2 sporophore growth training orientation
Treat that mycelia is covered with whole box face and deeply pricks cassette bottom, start to see light, but avoid sunlight direct projection.Temperature Should be 20~23 DEG C, humidity 80~85%.In sporophore late stage of culture, it is gradually increased the stream of room air Lead to and exchange, if culturing room's cultivation stage finds that pollution should be removed immediately.See about 40 days coremiums after light Long to 4-8cm, accidental have spore to produce, and cultivation terminates.Select neat and consistent, the most about 4~5cm left Right Cordyceps carries out the preparation of cordyceps militaris liquor.
Embodiment 2
A kind of cordyceps militaris liquor preparation method, the method comprises the steps:
Step a, injects the agar of 1.5% in wine holding container, and height is 1cm;
Step b, is inserted perpendicularly into Cordyceps militaris (L.) Link. in agar;
Step c, then in container, inject the agar of 2%, height is 0.5cm;
Step d, in container, injection Chinese liquor is to exceeding at Cordyceps 1~2cm, standing 30~60min, then Secondary injection Chinese liquor, at bottleneck 1~2cm, seals (as shown in Figure 1);
Step e, stores more than 45 days, filters, obtains cordyceps militaris liquor.
Embodiment 3
A kind of cordyceps militaris liquor preparation method, the method comprises the steps:
Step a, injects the agar of 1.5% in wine holding container, and height is 2cm;
Step b, is inserted perpendicularly into Cordyceps militaris (L.) Link. in agar;
Step c, then in container, inject the agar of 2.5%, height is 1cm;
Step d, in container, injection Chinese liquor is to exceeding at Cordyceps 1~2cm, standing 30~60min, then Secondary injection Chinese liquor, at bottleneck 1~2cm, seals;
Step e, stores more than 45 days, filters, obtains cordyceps militaris liquor.
Embodiment 4
A kind of cordyceps militaris liquor preparation method, the method comprises the steps:
Step a, injects the agar of 1.5% in wine holding container, and height is 1cm;
Step b, is inserted perpendicularly into Periostracum cicadae in agar;
Step c, then in container, inject the agar of 2%, height is 0.5cm;
Step e, in container, injection Chinese liquor is to exceeding at Cordyceps 1~2cm, standing 30~60min, then Secondary injection Chinese liquor, at bottleneck 1~2cm, seals;
Step d, stores more than 45 days, filters, obtains cordyceps militaris liquor.
Embodiment 5
A kind of cordyceps militaris liquor preparation method, the method comprises the steps:
Step a, injects the agar of 1.5% in wine holding container, and height is 2cm;
Step b, is inserted perpendicularly into Periostracum cicadae in agar;
Step c, then in container, inject the agar of 2.5%, height is 1cm;
Step d, in container, injection Chinese liquor is to exceeding at Cordyceps 1~2cm, standing 30~60min, then Secondary injection Chinese liquor, at bottleneck 1~2cm, seals;
Step e, stores more than 45 days, filters, obtains cordyceps militaris liquor.

Claims (7)

1. a cordyceps militaris liquor preparation method, it is characterised in that the method comprises the steps:
Step a, injects agar in wine holding container;
Step b, is inserted perpendicularly into Cordyceps in agar;
Step c, injects agar again in container;
Step d, injects Chinese liquor in container, seals;
Step e, stores more than 45 days;
Wherein, the concentration of agar described in step c is higher than the concentration of agar described in step a, and concentration difference is 0.5~1%.
2. preparation method as claimed in claim 1, it is characterised in that described in step a, agar height is 0.5~2cm.
3. preparation method as claimed in claim 1, it is characterised in that Cordyceps described in step b is pupa worm Grass or Periostracum cicadae.
4. preparation method as claimed in claim 1, it is characterised in that described in step c, agar height is 0.5~1cm.
5. preparation method as claimed in claim 1, it is characterised in that step d injects Chinese liquor to exceeding At Cordyceps 1~2cm, stand 30~60min, re-inject Chinese liquor at bottleneck 1~2cm.
6. preparation method as claimed in claim 1, it is characterised in that cordyceps militaris liquor passed through before consumption The step of filter.
7. the cordyceps militaris liquor that the preparation method as described in claim 1-6 is arbitrary prepares.
CN201510185793.3A 2015-04-17 2015-04-17 A kind of cordyceps militaris liquor preparation method Pending CN106148118A (en)

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Application Number Priority Date Filing Date Title
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Publications (1)

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CN106148118A true CN106148118A (en) 2016-11-23

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1162015A (en) * 1997-03-31 1997-10-15 汪景山 Production method of wine with shaped Chinese caterpillar fungus soaked in and product thereof
WO2006021126A1 (en) * 2004-07-09 2006-03-02 Shaosheng Sun Fresh living cordyceps wine
CN101153255A (en) * 2007-10-10 2008-04-02 张笑容 Cordyceps liquor and method of producing the same
CN101168713A (en) * 2007-10-30 2008-04-30 张笑容 Cordyceps sinensis wine and preparation method thereof
CN103981069A (en) * 2014-04-16 2014-08-13 田润生 Chinese caterpillar fungus wine preparation method
CN104419602A (en) * 2013-08-20 2015-03-18 浙江泛亚生物医药股份有限公司 Cordyceps sinensis liquor, preparation and application thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1162015A (en) * 1997-03-31 1997-10-15 汪景山 Production method of wine with shaped Chinese caterpillar fungus soaked in and product thereof
WO2006021126A1 (en) * 2004-07-09 2006-03-02 Shaosheng Sun Fresh living cordyceps wine
CN101153255A (en) * 2007-10-10 2008-04-02 张笑容 Cordyceps liquor and method of producing the same
CN101168713A (en) * 2007-10-30 2008-04-30 张笑容 Cordyceps sinensis wine and preparation method thereof
CN104419602A (en) * 2013-08-20 2015-03-18 浙江泛亚生物医药股份有限公司 Cordyceps sinensis liquor, preparation and application thereof
CN103981069A (en) * 2014-04-16 2014-08-13 田润生 Chinese caterpillar fungus wine preparation method

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Application publication date: 20161123