CN106146681B - A kind of method of low temperature continuous phase transistion extraction sea grass polysaccharide - Google Patents
A kind of method of low temperature continuous phase transistion extraction sea grass polysaccharide Download PDFInfo
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- CN106146681B CN106146681B CN201610555785.8A CN201610555785A CN106146681B CN 106146681 B CN106146681 B CN 106146681B CN 201610555785 A CN201610555785 A CN 201610555785A CN 106146681 B CN106146681 B CN 106146681B
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to Polyose extraction field, provides and a kind of doing the method that extractant extracts sea grass polysaccharide from Enteromorpha with water using continuous phase transistion technology.This method comprises the following steps：Enteromorpha raw material pulverization process；The Enteromorpha powder or slurry-mud that crush are added in extraction kettle；Extract enteromorpha powder or slurry-mud are being flowed through less than the liquid water extractant under extraction critical-temperature and critical pressure, subsequently into extraction-container, heating decompression, liquid water extractant is set to be converted to gaseous state, release polyoses extract, water is converted to liquid through cooling pressurization and reenters extraction kettle extraction, by product low temperature drying.The present invention has extraction low with parsing pressure and temp, and treating capacity is big, at low cost, product no solvent residue, and recovery rate is high, the high feature of purity.
Description
Technical field
The present invention relates to the extraction field of polysaccharide, more particularly to a kind of method of low temperature continuous phase transistion extraction sea grass polysaccharide.
Background technology
Since summer in 2008, Enteromorpha " green tide " is frequently broken out, and has more and more fiery trend, causes serious environment
Problem.The large-scale outbreak meeting shielded from sunlight of Enteromorpha, influences the growth of benthic algae；Dead Enteromorpha can also consume in seawater
The chemical substance of oxygen, Enteromorpha secretion is likely to also adversely affect other marine organisms, and Enteromorpha rots to send out smelly
Taste influences landscape and ambient enviroment.On the other hand, multiple beneficial substance is contained in Enteromorpha, algal polysaccharides therein have disease-resistant
The effects that malicious, antitumor, anti-mutation, radioresistance and strengthen immunity.Therefore, research and utilization Enteromorpha is conducive to turn waste into wealth, solution
Certainly while environmental problem, a kind of feasible material source is provided for dietetic food industry.In recent years, domestic and international brainstrust causes
Power achieves considerable progress in the research of the pharmacological function, bioactive functions of sea grass polysaccharide.
Currently, traditional seaweed Thick many candies extracting method mainly has：Water carries, acid carries and alkali carries.But these methods are due to technique
Cumbersome, recovery rate is low, it is difficult to industrialize, seriously limit the development and utilization of sea grass polysaccharide.
Invention content
In view of the above technical problems, the present invention provides a kind of method that sea grass polysaccharide is extracted in low temperature continuous phase transistion, the party
Method is simple for process, being capable of the efficient extraction for carrying out sea grass polysaccharide.
The technical solution adopted by the present invention is that：A kind of method of low temperature continuous phase transistion extraction sea grass polysaccharide, including following step
Suddenly：
（1）Enteromorpha is crushed；
（2）Extraction：By step（1）The Enteromorpha of middle smashing is added in extraction kettle, and extraction temperature is 60-80 DEG C, extraction pressure
Under conditions of power is 0.02-0.05MPa, extractant water flows through extraction kettle, continuous extraction 50- with the flow velocity of 100L/h-150L/h
120min；
（3）Parsing：Step（2）After extractant after extraction enters in extraction-container, 65-90 DEG C of resolution temperature parses pressure
0.01MPa-0.04MPa, extractant water mutually become gas by heating, depressurizing, and release extract sea grass polysaccharide, then pass through drop
Gas water becomes liquid and returns extraction kettle cycling extraction after temperature pressurization.
In the present invention, the Enteromorpha is dry Enteromorpha or wet Enteromorpha, and the dry Enteromorpha is smashed into Enteromorpha dry powder, the wet Enteromorpha
Wear into Enteromorpha slurry-mud.Further, the granularity of the Enteromorpha dry powder is 40-80 mesh.
Preferably, step（2）In, extraction temperature is 70 DEG C, extracting pressure 0.03Pa.
Preferably, step（2）In, the flow velocity of extractant water is 100L/h-120L/h, and the continuous extraction time is 80-
120min。
Preferably, step（3）In, resolution temperature is 80 DEG C, and parsing pressure is 0.02MPa.
Further, the method for low temperature continuous phase transistion of the invention extraction sea grass polysaccharide further includes step：
（4）It is dry：By step（3）It parses obtained sea grass polysaccharide and carries out low temperature drying.
It is an advantage of the invention that：
1. the sea grass polysaccharide extracting method of the present invention is to the of less demanding of raw material, Enteromorpha dry powder or Enteromorpha may be used in raw material
For slurry-mud as raw material, limitation is small.
2. the present invention prepares sea grass polysaccharide using continuous phase transistion abstraction technique formulation extraction, compared to current extraction work
Skill not only significantly reduces production cost, shortens extraction disengaging time, and technological process is simple, operability is strong, complete
The characteristics of full property and reproducible, extraction efficiency is high, has treating capacity big, and operating cost is low, no solvent residue, is, it can be achieved that big rule
Mould industrializes consecutive production.
3. water is derived from a wealth of sources as extractant, of low cost, environment friendly and pollution-free.The present invention continuous phase transistion abstraction technique with
Water is extractant, and extraction is low with parsing pressure and temp, and by extraction, the processes such as parsing successfully prepare sea grass polysaccharide, extraction effect
Rate is high, and the sea grass polysaccharide purity prepared is high, provides a kind of effective method for the extraction of sea grass polysaccharide, keeps the following Enteromorpha more
Sugar provides basis in the development and application of medical health field.
Specific implementation mode
Hereinafter, preferred embodiments of the present invention will be described, it should be understood that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
This method carries out according to the following steps using dry Enteromorpha as raw material:
One, dry Enteromorpha grinds are crossed 60 mesh and weeded out except coarse granule with disintegrating apparatus.
Two, 1kg Enteromorpha dry powder is taken（Moisture content about 14%）, it is added in extraction kettle (10L), 70 DEG C of extraction temperature, extracting pressure
0.03MPa, extractant water flow through Enteromorpha algae powder, continuous extraction 120 minutes with the speed of flow velocity 100L/h.Extractant enters parsing
In kettle, resolution temperature is 80 DEG C, pressure 0.02MPa.
Three, after extracting 120 minutes, extract is collected, low temperature drying, obtain Enteromorpha crude polysaccharide extract 136g, i.e., it is more
Sugar extract accounts for total solids 13.6%.
Four, after testing, in the Enteromorpha Thick many candies of extraction, polyoses content 56.2%.
Embodiment 2：
This method carries out according to the following steps using the fresh Enteromorpha in seaweed as raw material:
One, the fresh Enteromorpha of surface moisture will be dried（Water content about 90%）It is ground into slurry-mud with pulverizer.
Two, the Enteromorpha slurry-mud that 2kg is crushed is taken, is added in reaction kettle (10L), 80 DEG C of extraction temperature, extracting pressure
0.05MPa, extractant water flow through kelp, continuous extraction 60 minutes with the speed of flow velocity 150L/h.Extractant enters in extraction-container,
Resolution temperature is 90 DEG C, pressure 0.04MPa.
Three, extraction after sixty minutes, extract is collected, low temperature drying, obtained Enteromorpha crude polysaccharide extract 33.0g.It is more
Sugar extract accounts for about the 16.5% of total solids.
Four, after testing, in sea grass polysaccharide crude extract, polyoses content 64.1%.
Embodiment 3
This method carries out according to the following steps using dry Enteromorpha as raw material:
One, dry Enteromorpha grinds are crossed 80 mesh and weeded out except coarse granule with disintegrating apparatus.
Two, 1kg Enteromorpha dry powder is taken（Moisture content about 14%）, it is added in extraction kettle (10L), 60 DEG C of extraction temperature, extracting pressure
0.02MPa, extractant water flow through Enteromorpha algae powder, continuous extraction 100 minutes with the speed of flow velocity 120L/h.Extractant enters parsing
In kettle, resolution temperature is 70 DEG C, pressure 0.025MPa.
Three, after extracting 100 minutes, extract is collected, low temperature drying, obtain Enteromorpha crude polysaccharide extract 127g, i.e., it is more
Sugar extract accounts for total solids 12.7%.
Four, after testing, in the Enteromorpha Thick many candies of extraction, polyoses content 52.7%.
It can be that professional and technical personnel in the field realize or use that the above embodiment, which is intended to illustrate the present invention, to above-mentioned
Embodiment, which is modified, will be apparent to those skilled in the art, therefore the present invention includes but not limited to
The above embodiment, it is any to meet the claims or specification description, meet with principles disclosed herein and novelty,
The method of inventive features, technique, product, each fall within protection scope of the present invention.
Claims (7)
1. a kind of method of low temperature continuous phase transistion extraction sea grass polysaccharide, which is characterized in that include the following steps：
（1）Enteromorpha is crushed；
（2）Extraction：By step（1）The Enteromorpha of middle smashing is added in extraction kettle, and extraction temperature is 60-80 DEG C, and extracting pressure is
Under conditions of 0.02-0.05MPa, extractant water flows through extraction kettle, continuous extraction 50- with the flow velocity of 100L/h-150L/h
120min；
（3）Parsing：Step（2）After extractant after extraction enters in extraction-container, 65-90 DEG C of resolution temperature parses pressure
0.01MPa-0.04MPa, extractant water mutually become gas by heating, depressurizing, and release extract sea grass polysaccharide, then pass through drop
Gas water becomes liquid and returns extraction kettle cycling extraction after temperature pressurization.
2. the method for low temperature continuous phase transistion according to claim 1 extraction sea grass polysaccharide, which is characterized in that the Enteromorpha is
Dry Enteromorpha or wet Enteromorpha, the dry Enteromorpha are smashed into Enteromorpha dry powder, and the wet Enteromorpha wears into Enteromorpha slurry-mud.
3. the method for low temperature continuous phase transistion extraction sea grass polysaccharide according to claim 2, which is characterized in that the waterside dried lactuca
The granularity of powder is 40-80 mesh.
4. the method for low temperature continuous phase transistion extraction sea grass polysaccharide according to claim 1, which is characterized in that step（2）In,
Extraction temperature is 70 DEG C, extracting pressure 0.03MPa.
5. the method for low temperature continuous phase transistion extraction sea grass polysaccharide according to claim 1, which is characterized in that step（2）In,
The flow velocity of extractant water is 100L/h-120L/h, and the continuous extraction time is 80-120min.
6. the method for low temperature continuous phase transistion extraction sea grass polysaccharide according to claim 1, which is characterized in that step（3）In,
Resolution temperature is 80 DEG C, and parsing pressure is 0.02MPa.
7. the method for the low temperature continuous phase transistion extraction sea grass polysaccharide according to claim 1-6 any one, which is characterized in that
It further include step
（4）It is dry：By step（3）It parses obtained sea grass polysaccharide and carries out low temperature drying.
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| CN109232673A (en) * | 2018-08-08 | 2019-01-18 | 曹庸 | A kind of method of continuous phase transistion extraction shaddock fruit aurantiin and pectin |
| CN109096353A (en) * | 2018-08-08 | 2018-12-28 | 曹庸 | A kind of method that continuous phase transistion extracts aurantiamarin and pectin in citrus young fruit |
| CN110818810B (en) * | 2019-10-24 | 2021-01-26 | 华南农业大学 | Method for continuous phase-change extraction of ganoderma lucidum polysaccharide and method for evaluating extraction process based on online conductivity |
| CN113527527A (en) * | 2021-06-09 | 2021-10-22 | 深圳职业技术学院 | Method for extracting algal polysaccharide through continuous phase change |
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| CN103146486A (en) * | 2013-03-25 | 2013-06-12 | 广州绿萃生物科技有限公司 | Continuous phase-changed extraction method for orange peel oil |
| CN103349849A (en) * | 2013-07-22 | 2013-10-16 | 曹庸 | Multifunctional continuous phase transition extraction device |
| CN104403018A (en) * | 2014-11-26 | 2015-03-11 | 中国科学院海洋研究所 | Algal polysaccharide extraction method |
| CN104592409A (en) * | 2015-01-12 | 2015-05-06 | 陕西师范大学 | Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature |
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| US20100056772A1 (en) * | 2008-08-27 | 2010-03-04 | Zeilmann Thomas | Method for recovering arabinogalactan (LAG) from fibrous natural plant materials |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146486A (en) * | 2013-03-25 | 2013-06-12 | 广州绿萃生物科技有限公司 | Continuous phase-changed extraction method for orange peel oil |
| CN103349849A (en) * | 2013-07-22 | 2013-10-16 | 曹庸 | Multifunctional continuous phase transition extraction device |
| CN104403018A (en) * | 2014-11-26 | 2015-03-11 | 中国科学院海洋研究所 | Algal polysaccharide extraction method |
| CN104592409A (en) * | 2015-01-12 | 2015-05-06 | 陕西师范大学 | Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature |
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