CN106146680A - A kind of ultrasonic wave-coupled membrance separation grading system is for the method for different molecular weight lycium barbarum polysaccharide - Google Patents
A kind of ultrasonic wave-coupled membrance separation grading system is for the method for different molecular weight lycium barbarum polysaccharide Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Abstract
The present invention relates to a kind of ultrasonic wave-coupled membrance separation grading system method for different molecular weight lycium barbarum polysaccharide, the method utilizes ultrasonic extraction Fructus Lycii slag to obtain polysaccharide liquid, stand overnight, after nylon wire solid-liquid separation, centrifugal, first with 6.7 ten thousand molecular weight membrane ultrafiltration, the most again with 100,000 molecular weight membrane ultrafiltration, collect the ultrafiltrate that molecular weight is less than 6.7 ten thousand, 6.7 10 ten thousand and molecular weight is more than 100,000 respectively, obtain the lycium barbarum polysaccharide solution of three kinds of different molecular weights, through concentrating postlyophilization, obtain the lycium barbarum polysaccharide powder of different purposes.The method of the present invention obtains polysaccharide, have energy-conservation, save time, the advantage such as green, pollution-free, environmental protection, can be used for industry's enlarging production, after membrance separation, the lycium barbarum polysaccharide of the various molecular weight of classification is applicable to different food service industrys, having widened range of application, effect is obvious.
Description
Technical field
The present invention relates to the preparation method of a kind of lycium barbarum polysaccharide, be specifically related to a kind of ultrasonic wave-coupled membrance separation grading system standby
The method of different molecular weight lycium barbarum polysaccharide.
Technical background
Fructus Lycii (Lycium bararumL) is China's tradition integration of edible and medicinal herbs Chinese crude drug, has enriching yin and nourishing kidney, nourishing the liver to improve visual acuity
Etc. effect.In recent years, artificial culture Fructus Lycii quickly grows Chaidamu Basin, Qinghai Province, and to 2015, total cultivated area reached 300,000 mu,
Exceed Ningxia.Natural conditions that the Caidamu Basin is unique and clean ecological environment, have an area of hundred kilometer range without any pollution,
Day and night temperature is relatively big, and pest and disease damage is light, and spraying times is few, and the residual recall rate of agriculture is low, makes Qaidam Fructus Lycii have granule big and full, meat
Matter is plump and core is few, bright in colour and characteristics such as sweet in the mouth, is described as Qinghai-Tibet Platean " ruby ", has in the industry that " China Fructus Lycii is rather
In the summer, fine work Fructus Lycii is in Qinghai " say.New fresh fructus lycii is in process of production, it will substantial amounts of Fructus Lycii marc, generally this part occur
Marc will be taken as waste product to discard, and causes the biggest waste, adds the production cost of enterprise simultaneously.FRUCTUS LYCII after testing
In slag, polyoses content reaches 10.09%, lycium barbarum polysaccharide rich content, and clear efficacy progressively obtains accreditation both domestic and external, application
Constantly extension, possesses wide market prospect.
Lycium barbarum polysaccharide (Lycium Bararum polysaccharide, LBP) is the main composition of Fructus Lycii, has good
Good antioxidant activity, its main pharmacological has defying age, blood sugar lowering, blood fat reducing, enhancing immunologic function, resisting fatigue and resists swollen
Tumors etc., therefore LBP has the highest value of exploiting and utilizing as the Natural antioxidant.LBP composition is complex, and molecular weight can be from several
Thousand arrive hundreds of thousands.Huang Linjuan etc. obtain homogeneous group from preparation lycium barbarum polysaccharide LBP through DEAE-cellulose, Sephadex column chromatography
Dividing LbGP3, LbGP4 and LbGP5, molecular weight is respectively 2.37 × 104、9.25×104、21.48×104；Wherein LbGP4 Fructus Lycii
Sub-glycoconjugate LbGp4 obtains a molecular weight after U-elimination, column chromatography for separation and is up to branch's compound of polysaccharide of 180800
LbGp4-OL.LbGp4-OL glycosyl consists of n (Rha): n (Ara): n (Gal)=0.05: 1.33: 1.According to methylating, part
Acid hydrolysis and1HNMR、13CNMR analyzes, it is determined that its main structure, and carries out the immunocompetence of LbGp4 and LbGp4-OL
Research.Research finds: glycoconjugate LbGp4 and sugar chain LbGp4-OL after purification are not only anti-to normal mouse spleen cell proliferation
Should there is obvious facilitation, and Senescence-Accelerated Mouse SAMP8 and SAMR1 immunologic hypofunction also be had and significantly changes
Kind effect, shows that lycium barbarum polysaccharide has the effect directly promoting Spleen cell proliferation.From the point of view of their chemical property and molecular structure,
The molecular weight of Lycium Barbarum L. Glycoconjugate LbGp4 and sugar chain LbGp4-OL thereof is the biggest, and sugar chain has intensive branch, this
General vegetable polysaccharides is rare.Therefore, it there is stronger immunoregulation effect may (Huang Lin relevant with this structure
Beautiful etc., Acta Pharmaceutica Sinica, 1998.33(7): 512-516；SCI, 2001.3(22): 407-411).Peng Xuemei
Deng using column chromatography to obtain Fructus Lycii glycoconjugate LbGp2, through HPLC, CE, GC, SE and sugar content analysis, elementary analysis and ammonia
Base acid analysis etc. study its physicochemical property, and huge to abdominal cavity with research Lbp2 such as half intracorporal method, morphology counting method and colorimetrys
Phagocyte phagocytic function, the conversion of lymphocyte and the impact on mice serum HD50 etc..Found that LbGp2 is equal
One component, its molecular weight is 6.8 × 104, sugar content is 90.7%, and sugar consists of Ara-Gal 3: 4 (mol ratio), and containing 18
Plant natural amino acid.Immunocompetence test shows that Lbp2 has significant immunological enhancement；Also there is good antioxygen be turned into
With.Tian Gengyuan etc. extract the crude polysaccharides obtained from Lycium barbarum L., through DEAE-Cellulose and Sepadexe-100 column chromatography,
Obtain homogeneous wolfberry fruit candy albumen LbGP.Molecular weight is determined as 88kd by SDS-PAGE, and sugar content is 70%, and sugar consists of:
Gal:Gl=2.5:1.0:1.0 (mol ratio), and (Tian Gengyuan etc., biochemistry is with biological containing other 18 kinds of natural amino acids
Acta Physica Sinica, 1995,27(2): 201-206).The Ultra filtration membrane of 5 kinds of PSPPs (MWCO) such as Yao Ruiqi
LBP, phend-sulphuric acid measures the content of different molecular weight polysaccharide, and iron ion reducing power (FRAP) method measures antioxidant activity.Knot
Fruit has obtained the LBP sample liquid of 6 kinds of different molecular weights.Molecular weight LBP below 5000 occupies the majority, and reaches 74.5%;Molecular weight
Relatively low, still less than 7% more than 30,000.The LBP antioxidant activity of MW3 ten thousand-10 ten thousand is the strongest, and other 5 kinds of LBP also have certain journey
Degree antioxidant activity (Yao Ruiqi etc., food industry science and technology, 2008,29:89-91).
The extraction of vegetable polysaccharides is varied, but extracts the method but basic simlarity of active polysaccharide, is all to utilize polysaccharide
Soluble in water, sour, alkali and feature insoluble in organic solvents such as alcohol, but meet green, environmental protection, nontoxic, possess higher extracted effect
The industrialization extraction of the active polysaccharide of rate, purification method little.
The purification process of water solublity lycium barbarum polysaccharide typically has ethanol precipitation, column chromatography and Sevage to remove isolating protein
Method.Ethanol precipitation utilizes most glucide organic molten by adding insoluble in ethanol, this character of acetone and other organic solvent
Agent is precipitated out the polysaccharose substance insoluble in solvent, thus reach with solubility small-molecule substance (the little molecule of such as pigment,
Salt micromolecular etc.) purpose that separates obtains polysaccharide crude；Column chromatography has higher separation efficiency, but gel column price is held high
Expensive, commonly used prepare a small amount of sterling in the lab, be not suitable for industrially large-scale use；Sevage removes isolating protein
There is organic reagent and consume big in method, operation complexity, the shortcomings such as polysaccharide loss is serious.Ultrafiltration extraction purification polysaccharide has technique stream
Journey is simple, product purity high.Membrane separation technique is owing to having without phase transition process, the advantage such as pollution-free, progressively in industry
Metaplasia uses in producing.
Summary of the invention
It is an object of the invention to provide a kind of ultrasonic wave-coupled membrance separation grading system side for different molecular weight lycium barbarum polysaccharide
Method, the method has energy-conservation, green, environmental protection, free of contamination feature.The method of the present invention makes Fructus Lycii slag polysaccharide downstream hierarchy extract
Preparation, it is possible to according to the feature of Fructus Lycii crude polysaccharides, make full use of inorganic nylon wire and the feature of organic ultrafilter membrane, optionally cut
Stay purified concentration lycium barbarum polysaccharide, meet the industrialized production need of the lycium barbarum polysaccharide preparing high-purity, different application effect on a large scale
Ask.
The technical scheme is that a kind of ultrasonic wave-coupled membrance separation grading system side for different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that include step:
Step 1, dries Fructus Lycii slag, pulverizes with pulverizer, it is thus achieved that Fructus Lycii ground-slag end；
Step 2, to step 1 Fructus Lycii ground-slag end by mass volume ratio be 1:20-30 add solvent, soaking at room temperature 3h, obtain feed liquid；Material
Liquid is 200-300w at ultrasonic power, ultrasonic extraction Fructus Lycii slag 3 times, each 20-30min, merging filtrate, it is thus achieved that lycium barbarum polysaccharide
Extracting solution；
Step 3, the lycium barbarum polysaccharide extracting solution to step 2, through 8 layers of nylon net filter of 60-75 mesh, remove seed of Fructus Lycii and insoluble matter；
Step 4, removes the lycium barbarum polysaccharide extracting solution after seed of Fructus Lycii and insoluble matter and stands 24 hours, collect supernatant, abandon step 3
Slag；
Step 5, the supernatant collecting step 4, on centrifugal separator, turns with 7000-9000, centrifugal 15-25min carries out solid
Liquid separates, and collects supernatant, waste, it is thus achieved that lycium barbarum polysaccharide clear liquid；
Step 6, to the lycium barbarum polysaccharide clear liquid of step 5 gained again through the membrance separation grading purification of different molecular weight, obtains difference
The lycium barbarum polysaccharide solution of molecular weight；The membrance separation grading purification of described different molecular weight refers to first surpassing with 6.7 ten thousand molecular weight
Ultrafiltration through membranes, it is thus achieved that permeate and trapped fluid, trapped fluid is again with the ultrafilter membrane ultrafiltration of 100,000 molecular weight, it is thus achieved that permeate and retaining
Liquid, collects the molecular weight ultrafiltrate less than 6.7 ten thousand, 6.7 ten thousand-10 ten thousand with more than 100,000 the most respectively；
Step 7, concentrated rear dry to the ultrafiltrate of step 6 gained, prepare the molecular weight water solublity Fructus Lycii less than 6.7 ten thousand respectively
Polysaccharide powder, molecular weight are the water solublity lycium barbarum polysaccharide powder between 6.7-10 ten thousand and the molecular weight water solublity lycium barbarum polysaccharide more than 100,000
Powder.
The residue during Fructus Lycii series of products is prepared by Fructus Lycii Zha Shi Fructus Lycii manufacturing enterprise in described step 1.
Solvent in described step 2 is water.
The extraction equipment used by ultrasonic extraction in described step 2 is ultrasonic cleaner.
Membrance separation in described step 6 uses hollow-fibre membrane lab scale equipment.
During the concentration of the ultrafiltrate in described step 7, concentrating the equipment used is vacuum concentration equipment.
Being dried as being spray-dried or vacuum lyophilization in described step 7.
The invention have the advantage that the present invention uses membrane separation technique to separate, according to the size of lycium barbarum polysaccharide molecular weight,
The film selecting molecular interception amount different separates, and can obtain the lycium barbarum polysaccharide that molecular size range is different, prominent every kind of Fructus Lycii
The application effect of polysaccharide, has widened the range of application of lycium barbarum polysaccharide.Synergistic supersonic wave improves lycium barbarum polysaccharide extraction simultaneously
Effect, and save time, efficient, energy-conservation, green and environmental protection.
Below by specific embodiment, the present invention is described further, but not as limiting to the invention.
Detailed description of the invention
Embodiment 1
A kind of ultrasonic wave-coupled membrance separation grading system is for the method for different molecular weight lycium barbarum polysaccharide, it is characterised in that include step:
Step 1, dries Fructus Lycii slag, pulverizes with pulverizer, it is thus achieved that Fructus Lycii ground-slag end；
Step 2, to step 1 Fructus Lycii ground-slag end by mass volume ratio be 1:20 add solvent, soaking at room temperature 3h obtains feed liquid；Feed liquid exists
Ultrasonic power is 200w, ultrasonic extraction Fructus Lycii slag 3 times, each 20min, merging filtrate, it is thus achieved that lycium barbarum polysaccharide extracting solution；Described
Solvent is water；
Step 3, the lycium barbarum polysaccharide extracting solution to step 2, through 8 layers of nylon net filter of 60 mesh, remove seed of Fructus Lycii and insoluble matter；
Step 4, removes the lycium barbarum polysaccharide extracting solution after seed of Fructus Lycii and insoluble matter and stands 24 hours, collect supernatant, abandon step 3
Slag；
Step 5, the supernatant that step 4 is collected on centrifugal separator, with 7000 turns, centrifugal 15min carry out solid-liquid separation, receive
Collection supernatant, waste, it is thus achieved that lycium barbarum polysaccharide clear liquid；
Step 6, to the lycium barbarum polysaccharide clear liquid of step 5 gained again through the membrance separation grading purification of different molecular weight, obtains difference
The lycium barbarum polysaccharide solution of molecular weight；The membrance separation grading purification of described different molecular weight refers to first surpassing with 6.7 ten thousand molecular weight
Ultrafiltration through membranes, it is thus achieved that permeate (molecular weight ultrafiltrate less than 6.7 ten thousand) and the trapped fluid (molecular weight ultrafiltration more than 6.7 ten thousand
Liquid), trapped fluid is again with the ultrafilter membrane ultrafiltration of 100,000 molecular weight, it is thus achieved that permeate (molecular weight is at the ultrafiltrate of 6.7-10 ten thousand) and cut
Stay the liquid ultrafiltrate of 100,000 (molecular weight more than), collect the most respectively molecular weight less than 6.7 ten thousand, molecular weight 6.7 ten thousand-10 ten thousand and point
The son amount ultrafiltrate more than 100,000；
Step 7, concentrated rear dry to the ultrafiltrate of step 6 gained, prepare the molecular weight water solublity Fructus Lycii less than 6.7 ten thousand respectively
Polysaccharide powder, molecular weight are the water solublity lycium barbarum polysaccharide powder between 6.7-10 ten thousand and the molecular weight water solublity lycium barbarum polysaccharide more than 100,000
Powder.With glucose for mark product, phend-sulphuric acid is used to measure polyoses content；With bovine serum albumin as standard specimen, use coomassie bright
Blue laws measures protein content；With galacturonic acid as standard specimen, the gala furfural acid using carbazol-sulfuric acid method to measure in sample contains
Amount, the results are shown in Table 1.
The polysaccharide of table 1 different molecular weight lycium barbarum polysaccharide, protein, the content of gala furfural acid
Embodiment 2
A kind of ultrasonic wave-coupled membrance separation grading system is for the method for different molecular weight lycium barbarum polysaccharide, it is characterised in that include step:
Step 1, dries Fructus Lycii slag, pulverizes with pulverizer, it is thus achieved that Fructus Lycii ground-slag end；
Step 2, to step 1 Fructus Lycii ground-slag end by mass volume ratio be 1:25 add solvent, soaking at room temperature 4h, obtain feed liquid；Feed liquid
It is 250w at ultrasonic power, ultrasonic extraction Fructus Lycii slag 3 times, each 25min, merging filtrate, it is thus achieved that lycium barbarum polysaccharide extracting solution；Institute
Stating solvent is water；
Step 3, the lycium barbarum polysaccharide extracting solution to step 2, through 8 layers of nylon net filter of 65 mesh, remove seed of Fructus Lycii and insoluble matter；
Step 4, removes the lycium barbarum polysaccharide extracting solution after seed of Fructus Lycii and insoluble matter and stands 24 hours, collect supernatant, abandon step 3
Slag；
Step 5, the supernatant that step 4 is collected on centrifugal separator, with 8000 turns, centrifugal 20min carry out solid-liquid separation, receive
Collection supernatant, waste, it is thus achieved that lycium barbarum polysaccharide clear liquid；
Step 6, to the lycium barbarum polysaccharide clear liquid of step 5 gained again through the membrance separation grading purification of different molecular weight, obtains difference
The lycium barbarum polysaccharide solution of molecular weight；The membrance separation grading purification of described different molecular weight refers to first surpassing with 6.7 ten thousand molecular weight
Ultrafiltration through membranes, it is thus achieved that permeate (molecular weight ultrafiltrate less than 6.7 ten thousand) and the trapped fluid (molecular weight ultrafiltration more than 6.7 ten thousand
Liquid), trapped fluid is again with the ultrafilter membrane ultrafiltration of 100,000 molecular weight, it is thus achieved that the permeate ultrafiltrate of 6.7 ten thousand-10 ten thousand (molecular weight) and
The trapped fluid ultrafiltrate of 100,000 (molecular weight more than), collect the most respectively molecular weight less than 6.7 ten thousand, molecular weight 6.7 ten thousand-10 ten thousand and
Ultrafiltrate more than 100,000 molecular weight；
Step 7, concentrated rear dry to the ultrafiltrate of step 6 gained, prepare the molecular weight water solublity Fructus Lycii less than 6.7 ten thousand respectively
Polysaccharide powder, molecular weight are that the water solublity lycium barbarum polysaccharide powder between 6.7 ten thousand-10 ten thousand and the molecular weight water solublity Fructus Lycii more than 100,000 is many
Icing Sugar.With glucose for mark product, phend-sulphuric acid is used to measure polyoses content；With bovine serum albumin as standard specimen, use coomassie
Light blue method measures protein content；With galacturonic acid as standard specimen, carbazol-sulfuric acid method is used to measure the gala furfural acid in sample
Content, the results are shown in Table 2.
The polysaccharide of table 2 different molecular weight lycium barbarum polysaccharide, protein, the content of gala furfural acid
Embodiment 3
A kind of ultrasonic wave-coupled membrance separation grading system is for the method for different molecular weight lycium barbarum polysaccharide, it is characterised in that include step:
Step 1, dries Fructus Lycii slag, pulverizes with pulverizer, it is thus achieved that Fructus Lycii ground-slag end；
Step 2, to step 1 Fructus Lycii ground-slag end by mass volume ratio be 1:30 add solvent, soaking at room temperature 5h, obtain feed liquid；Feed liquid
It is 300w at ultrasonic power, ultrasonic extraction Fructus Lycii slag 3 times, each 30min, merging filtrate, it is thus achieved that lycium barbarum polysaccharide extracting solution；Institute
Stating solvent is water；
Step 3, the lycium barbarum polysaccharide extracting solution to step 2, through 8 layers of nylon net filter of 70 mesh, remove seed of Fructus Lycii and insoluble matter；
Step 4, removes the lycium barbarum polysaccharide extracting solution after seed of Fructus Lycii and insoluble matter and stands 24 hours, collect supernatant, abandon step 3
Slag；
Step 5, the supernatant that step 4 is collected on centrifugal separator, with 9000 turns, centrifugal 30min carry out solid-liquid separation, receive
Collection supernatant, waste, it is thus achieved that lycium barbarum polysaccharide clear liquid；
Step 6, to the lycium barbarum polysaccharide clear liquid of step 5 gained again through the membrance separation grading purification of different molecular weight, obtains difference
The lycium barbarum polysaccharide solution of molecular weight；The membrance separation grading purification of described different molecular weight refers to first surpassing with 6.7 ten thousand molecular weight
Ultrafiltration through membranes, it is thus achieved that permeate (molecular weight ultrafiltrate less than 6.7 ten thousand) and the trapped fluid (molecular weight ultrafiltration more than 6.7 ten thousand
Liquid), trapped fluid is again with the ultrafilter membrane ultrafiltration of 100,000 molecular weight, it is thus achieved that the permeate ultrafiltrate of 6.7 ten thousand-10 ten thousand (molecular weight) and
The trapped fluid ultrafiltrate of 100,000 (molecular weight more than), collect the most respectively molecular weight less than 6.7 ten thousand, molecular weight 6.7 ten thousand-10 ten thousand and
Ultrafiltrate more than 100,000；
Step 7, concentrated rear dry to the ultrafiltrate of step 6 gained, prepare the molecular weight water solublity Fructus Lycii less than 6.7 ten thousand respectively
Polysaccharide powder, molecular weight are that the water solublity lycium barbarum polysaccharide powder between 6.7 ten thousand-10 ten thousand and the molecular weight water solublity Fructus Lycii more than 100,000 is many
Icing Sugar.With glucose for mark product, phend-sulphuric acid is used to measure polyoses content；With bovine serum albumin as standard specimen, use coomassie
Light blue method measures protein content；With galacturonic acid as standard specimen, carbazol-sulfuric acid method is used to measure the gala furfural acid in sample
Content, the results are shown in Table 3.
The polysaccharide of table 3. different molecular weight lycium barbarum polysaccharide, protein, the content of gala furfural acid
Embodiment 4
Comparative example: method 1 is to prepare different molecular weight lycium barbarum polysaccharide with embodiment 3；Method 2 and embodiment 3 prepare lycium barbarum polysaccharide
Step essentially identical, except for the difference that method 2 precipitate with ethanol instead of membrance separation；Method 3 and embodiment 3 prepare the step of lycium barbarum polysaccharide
Essentially identical, except for the difference that method 3 does not has precipitate with ethanol or membrance separation.
Contrast test table 4
Contrast test table 4 shows, from the point of view of polyoses content, precipitate with ethanol > membrance separation > not precipitate with ethanol, the polysaccharide prepared by chemical reagent
The biggest with polysaccharide difference prepared by membrance separation；From the point of view of protein content, not precipitate with ethanol > precipitate with ethanol and membrance separation, prepared by membrance separation
Polysaccharide and the proteinpolysaccharide content prepared of chemical reagent close；From the point of view of galacturonic acid content, not precipitate with ethanol > precipitate with ethanol and film
Separating, polysaccharide prepared by membrance separation and polysaccharide galacturonic acid content prepared by chemical reagent are close.Show there is no any chemistry
The membrance separation preparation technology of reagent is feasible.
Illustrate from above-mentioned contrast test table 4, use standby different points of a kind of ultrasonic wave-coupled membrance separation grading system of the present invention
The method of son amount lycium barbarum polysaccharide is effective.
The polysaccharide that the inventive method obtains, have energy-conservation, save time, the advantage such as pollution-free, green, environmental protection, can be used for work
Industry metaplasia is produced, and after membrance separation, the lycium barbarum polysaccharide of the various molecular weight of grading purification is applicable to different food service industrys, and having widened should
By scope, effect is obvious.
Processing step that the present embodiment describes the most in detail or parts belong to conventional means or the known features of the industry, here
Describe the most one by one.
Claims (7)
1. a ultrasonic wave-coupled membrance separation grading system is for the method for different molecular weight lycium barbarum polysaccharide, it is characterised in that include step
Rapid:
Step 1, dries Fructus Lycii slag, pulverizes with pulverizer, it is thus achieved that Fructus Lycii ground-slag end；
Step 2, to step 1 Fructus Lycii ground-slag end by mass volume ratio be 1:20-30 add solvent, soaking at room temperature 3h-5h, obtain material
Liquid；Feed liquid is 200-300w at ultrasonic power, ultrasonic extraction Fructus Lycii slag 3 times, each 20-30min, merging filtrate, it is thus achieved that Chinese holly
Qi polysaccharide extraction liquid；
Step 3, the lycium barbarum polysaccharide extracting solution to step 2, through 8 layers of nylon net filter of 60-75 mesh, remove seed of Fructus Lycii and insoluble matter；
Step 4, removes the lycium barbarum polysaccharide extracting solution after seed of Fructus Lycii and insoluble matter and stands 24 hours, collect supernatant, abandon step 3
Slag；
Step 5, the supernatant collecting step 4, on centrifugal separator, turns with 7000-9000, centrifugal 15-25min carries out solid
Liquid separates, and collects supernatant, waste, it is thus achieved that lycium barbarum polysaccharide clear liquid；
Step 6, to the lycium barbarum polysaccharide clear liquid of step 5 gained again through the membrance separation grading purification of different molecular weight, obtains difference
The lycium barbarum polysaccharide solution of molecular weight；The membrance separation grading purification of described different molecular weight refers to first surpassing with 6.7 ten thousand molecular weight
Ultrafiltration through membranes, it is thus achieved that permeate and trapped fluid, trapped fluid is again with the ultrafilter membrane ultrafiltration of 100,000 molecular weight, it is thus achieved that permeate and retaining
Liquid, collects molecular weight the most respectively and is less than 6.7 ten thousand, 6.7 ten thousand-10 ten thousand and is more than the lycium barbarum polysaccharide ultrafiltrate of 100,000 molecular weight；
Step 7, concentrated rear dry to the ultrafiltrate of step 6 gained, prepare the molecular weight water solublity Fructus Lycii less than 6.7 ten thousand respectively
Polysaccharide powder, molecular weight are that the water solublity lycium barbarum polysaccharide powder between 6.7 ten thousand-10 ten thousand and the molecular weight water solublity Fructus Lycii more than 100,000 is many
Icing Sugar.
A kind of ultrasonic wave-coupled membrance separation grading system the most according to claim 1 is for the side of different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that: the residue during Fructus Lycii series of products is prepared by the Fructus Lycii Zha Shi Fructus Lycii manufacturing enterprise in described step 1.
A kind of ultrasonic wave-coupled membrance separation grading system the most according to claim 1 is for the side of different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that: the solvent in described step 2 is water.
A kind of ultrasonic wave-coupled membrance separation grading system the most according to claim 1 is for the side of different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that: the extraction equipment used by ultrasonic extraction in described step 2 is ultrasonic cleaner.
A kind of ultrasonic wave-coupled membrance separation grading system the most according to claim 1 is for the side of different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that: the membrane separation plant in described step 6 is hollow-fibre membrane lab scale equipment.
A kind of ultrasonic wave-coupled membrance separation grading system the most according to claim 1 is for the side of different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that: during the concentration of the ultrafiltrate in described step 7, concentrating the equipment used is vacuum concentration equipment.
A kind of ultrasonic wave-coupled membrance separation grading system the most according to claim 1 is for the side of different molecular weight lycium barbarum polysaccharide
Method, it is characterised in that: being dried as being spray-dried or vacuum lyophilization in described step 7.
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| CN107279632A (en) * | 2017-07-31 | 2017-10-24 | 广西健美乐食品有限公司 | One kind fat-reducing banana beverage and preparation method thereof |
| CN109393458A (en) * | 2018-10-16 | 2019-03-01 | 宁夏沃福百瑞枸杞产业股份有限公司 | A kind of preparation method of Lycium chinense glycopeptide particle electuary |
| CN112457426A (en) * | 2020-12-10 | 2021-03-09 | 中国科学院兰州化学物理研究所 | Method for simultaneously extracting different lycium barbarum polysaccharide components |
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| CN107279632A (en) * | 2017-07-31 | 2017-10-24 | 广西健美乐食品有限公司 | One kind fat-reducing banana beverage and preparation method thereof |
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