CN106139257B - A kind of orientation silk fibroin hydrogel and preparation method thereof - Google Patents
A kind of orientation silk fibroin hydrogel and preparation method thereof Download PDFInfo
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Abstract
A kind of preparation method being orientated silk fibroin hydrogel, domestic silkworm silk obtains silk fibroin water solution after degumming, dissolution, dialysis, further include following steps: (1) silk fibroin water solution that concentration is 10g/L~60g/L and the aqueous surfactant solution that concentration is 35g/L~100g/L is respectively configured, then stores silk fibroin water solution and aqueous surfactant solution under the conditions of 2 DEG C -8 DEG C of temperature respectively；(2) ratio that silk fibroin water solution and aqueous surfactant solution are 10:1 by volume is uniformly mixed and obtains mixed liquor, mixed liquor is poured into circular die, it is circular stirring-head that stirring rod on blender, which connects bottom surface, it is heated with thermostat water bath, makes temperature and 10S of the mixed solution at 10 DEG C~50 DEG C‑1~1000S‑1Shear rate under shear 5min~120min, obtain orientating type silk fibroin hydrogel.Orientating type silk fibroin hydrogel produced by the present invention has good biocompatibility and degradability, and intensity is high, and elasticity is good, cut resistant.
Description
Technical field
The present invention relates to a kind of hydrogel materials and preparation method thereof, more particularly to a kind of orientation fibroin albumen water-setting
Glue and preparation method thereof, it can be used as cell scaffold material, tissue renovation material etc., be applied to bio-medical field.
Background technique
Hydrogel is a kind of polymer with network structure that can absorb and possess large quantity of moisture, in the net of polymer
Contain some hydrophobic grouping and hydrophilic residue, hydrophilic residue and water molecules in network structure, hydrone is connected to netted
Inside, and hydrophobic residue water-swellable keeps certain shape.Hydrogel has good biocompatibility, gas permeability and cell
Adhesiveness is applied to artificial skin, tissue engineering material, drug release, micropin etc. extensively at present.
Fibroin albumen is a kind of safe and reliable natural high molecular material, nontoxic, nonirritant, has good biology
Compatibility and biodegradability.Fibroin albumen has the ability for guiding netted connection tissue to generate, can effectively induced tissue
Reconstruction and palingenesis.Silk fibroin hydrogel is that gelation preparation occurs under certain condition for regenerated silk fibroin solution
It forms.Regenerated silk fibroin solution gels process is mainly by factors such as the concentration of silk fibroin solution, temperature, pH, metal ions
It influences.It can promote its gelation process by changing silk fibroin solution concentration, pH, addition metal ion etc..There is document (food section
Learn, 2007,28 (12): 58-62) report, it is easily formed gel when the concentration of silk fibroin solution is greater than 10%, concentration is less than 10%
When take a long time that gel could be formed.There are also document (Chemical Society, 2001,42:294-295) report it is low
Metal ion (the Ca of concentration2+) silk fibroin protein solution gelation can be promoted, and the metal ion of high concentration results in fibroin egg
White partly precipitated and destroy gel structure.
Before this patent, Chinese invention patent (CN1158338C) discloses a kind of fibroin albumen for being chemically crosslinked structure
The preparation method of hydrogel, spongy silk fibroin hydrogel material using the pH of adjustment silk fibroin water solution, and is mixed into ring
Oxygen compound is as crosslinking agent, and chilled, solidification forms gel.Since epoxide has certain toxicity, although using
WATER-WASHING METHOD washes away unreacted epoxy crosslinking agent, but can still have remaining epoxy crosslinking agent in gel, to reduce fibroin egg
The biocompatibility of plain boiled water gel rubber material.Chinese invention patent (CN101502670) discloses a kind of silk fibroin hydrogel
Preparation method forms hydrogel using the method for ultrasonic oscillation in a short time, but is hardly formed the water-setting with orientation
Glue.
Summary of the invention
In order to solve the above technical problems, the purpose of the present invention is to provide a kind of good biocompatibilities, safe and non-toxic and tool
There is a kind of orientation silk fibroin hydrogel and preparation method thereof of orientation.
Technical problem of the invention is mainly addressed by the following technical programs:
The invention discloses a kind of orientation silk fibroin hydrogel and preparation method thereof, include the following steps: that domestic silkworm silk passes through
Silk fibroin water solution is obtained after degumming, dissolution, dialysis, further includes following steps:
(1) it is 35g/L~100g/L's that silk fibroin water solution that concentration is 10g/L~60g/L and concentration, which is respectively configured,
Aqueous surfactant solution, then by silk fibroin water solution and aqueous surfactant solution respectively in 2 DEG C -8 DEG C of temperature strip
It is stored under part；
(2) ratio that silk fibroin water solution and aqueous surfactant solution are 10:1 by volume is uniformly mixed and is obtained
Mixed liquor pours into mixed liquor in circular die, and it is discoid stirring-head that the stirring rod on blender, which is connected with bottom surface, with perseverance
The heating of warm water bath, makes temperature and 20S of the mixed solution at 10 DEG C~50 DEG C-1~1000S-1Shear rate under shear 5min
~120min obtains orientation silk fibroin hydrogel.
Further, the stirring-head bottom shape and circular die shape match and the stirring-head can be in circle
It is rotated horizontally in mold, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
Further, the surfactant is anionic surfactant or nonionic surfactant.
Further, the anionic surfactant are as follows: sodium bacillus subtilis lipopeptide, N- lauroyl musculamine acid sodium, stearic acid
Sodium, potassium stearate, magnesium stearate, stearoyl lactate, stearyl lactate, casein sodium, aliphatic alcohol polyethenoxy sulfuric acid
One of sodium is several.
Further, the nonionic surfactant are as follows: sorbitan monostearate, three stearic acid of sorbitol anhydride
Ester, sorbitan mono-oleic acid ester, sorbitol anhydride monopalmitate, sucrose fatty ester, polysorbate, propylene glycol alginate
One of or it is several.
In addition, being prepared the present invention also provides a kind of orientation silk fibroin hydrogel using the above method.
The invention has the advantages that
(1) present invention is used as gel bodies using fibroin albumen, because it is with good biocompatibility and degradability, only
Special mechanical performance and physicochemical property and it is from a wealth of sources easy to process the advantages that, therefore can be widely used for artificial skin, artificial blood vessel,
The medical aspect such as artificial neuron, cell scaffold material.
(2) surfactant used by this patent is the food additives for meeting national safety standard, nontoxic, stingless
Swash property smell, biocompatibility and the safety of Silk fibroin gel material will not be reduced；Can degrade, and catabolite without
Poison, non-immunogenicity, are the ideal materials as cytoskeleton.
(3) orientating type silk fibroin hydrogel provided by the present invention, intensity is high, and elasticity is good, cut resistant, to meet orientation
The reparation of type tissue provides a kind of approach, such as musculature, nerve fiber.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention and the accompanying drawings.
Detailed description of the invention
Fig. 1 is a kind of orientation silk fibroin hydrogel stereoscan photograph made from implementation one, and wherein A is that the hydrogel is put
Surface texture pattern after big 50 times, B are the surface texture patterns amplified after 100 times, and C is the surface texture amplified after 200 times
Pattern, D is the surface texture pattern amplified after 400 times, and the surface texture pattern of A, B, C and D are each parallel to differently- oriented directivity.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiments of the present invention will be described in further detail.Implement below
Example is not intended to limit the scope of the invention for illustrating the present invention.
Embodiment one:
The preparation step of silk fibroin hydrogel provided in this embodiment is as follows:
1,80 grams of silkworm raw silks are weighed, to 4L mass concentration be 0.25g/L sodium carbonate and mass concentration is 0.75g/L carbonic acid
After hydrogen sodium mixed aqueous solution boils, domestic silkworm silk is put into the solution boiled, the slightly boiled processing under the conditions of 98 DEG C~100 DEG C
It after 30min, is washed with deionized, is repeated twice aforesaid operations, make natural silk degumming, after finally being cleaned with deionized water, drying
60 DEG C of drying, can be obtained pure silk cellulose fiber in case；
2, the lithium-bromide solution of 9.3mol/L is used to dissolve with the bath raio of 1:8 pure silk cellulose fiber, in constant temperature magnetic force
It is cooled to room temperature after stirring and dissolving 30min at 65 ± 2 DEG C in blender to get fibroin albumen mixed solution is arrived；
3, by fibroin albumen mixed solution after cooling be fitted into molecular cut off be 8-10kDa bag filter in, spend from
After sub- water is dialysed 3 days, is filtered through absorbent cotton, pure silk fibroin protein solution can be obtained；
4, the concentration for adjusting silk fibroin protein solution is 31g/L, i.e. component A is deposited at a temperature of 4 DEG C；
5, compound concentration is the surfaction sodium solution of 100g/L, i.e. component B is deposited at a temperature of 4 DEG C；
6, above-mentioned component A and component B are uniformly mixed with the volume ratio of 10:1, mixed liquor is poured into circular die, is stirred
Mixing the stirring rod on device and connecting bottom surface is circular stirring-head, is heated with thermostat water bath, makes temperature of the mixed solution at 37 DEG C
And 100S-1Shear rate under shear 30min, obtain molding orientation silk fibroin hydrogel.
Above-mentioned stirring-head bottom shape and circular die shape match and guarantee stirring-head can in circular die water
Flat rotation, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
As shown in Figure 1, being orientation silk fibroin hydrogel stereoscan photograph obtained above, wherein A is the hydrogel
Surface texture pattern after 50 times of amplification, B are the surface texture patterns amplified after 100 times, and C is the surface knot after amplifying 200 times
Looks are configured, D is the surface texture pattern amplified after 400 times, and the surface texture pattern of A, B, C and D are each parallel to differently- oriented directivity.
Mechanics Performance Testing is carried out using TMS-PRO Texture instrument to the hydrogel, test result is as follows shown in table:
1 compressive strength of table
| Compression direction | Compressive strength (KPa) | Standard deviation |
| Differently- oriented directivity | 281.15 | 18.98 |
| Non-oriented direction | 126.4 | 16.62 |
Table 2 cuts intensity
| Cut direction | It cuts intensity (KPa) | Standard deviation |
| Perpendicular to differently- oriented directivity | 36.80 | 3.92 |
| It is parallel to differently- oriented directivity | 20.12 | 3.12 |
As can be seen from Table 1 and Table 2, the compressive strength of the hydrogel differently- oriented directivity is strong greater than the compression in non-oriented direction
Degree；It is greater than perpendicular to the cutting intensity of differently- oriented directivity and is parallel to the cutting intensity of differently- oriented directivity, the hydrogel is perpendicular to orientation side
To cut resistant, it is easy to be cut into lamella along differently- oriented directivity.
Example two:
1,80 grams of silkworm raw silks are weighed, to 4L mass concentration be 0.25g/L sodium carbonate and mass concentration is 0.75g/L carbonic acid
After hydrogen sodium mixed aqueous solution boils, under the conditions of 98 DEG C~100 DEG C after slightly boiled processing 30min, it is washed with deionized, repeats
2 aforesaid operations, make natural silk degumming, and after finally being cleaned with deionized water, it is fine that pure silk element can be obtained in 60 DEG C of drying in an oven
Dimension；
2, the lithium-bromide solution of 9.3mol/L is used to dissolve with the bath raio of 1:8 pure silk cellulose fiber, in constant temperature magnetic force
It is cooled to room temperature after stirring and dissolving 30min at 65 ± 2 DEG C in blender to get fibroin albumen mixed solution is arrived；
3, by fibroin albumen mixed solution after cooling be fitted into molecular cut off be 8-10kDa bag filter in, spend from
After sub- water is dialysed 3 days, is filtered through absorbent cotton, pure silk fibroin protein solution can be obtained；
4, the concentration for adjusting silk fibroin protein solution is 42g/L, i.e. component A is deposited at a temperature of 4 DEG C；
5, compound concentration is the AES solution of 90g/L, i.e. component B is deposited at a temperature of 4 DEG C；
6, above-mentioned component A and component B are uniformly mixed with the volume ratio of 10:1, mixed liquor is poured into circular die, is stirred
Mixing the stirring rod on device and connecting bottom surface is circular stirring-head, is heated with thermostat water bath, makes temperature of the mixed solution at 37 DEG C
And 75S-1Shear rate under shear 10min, obtain molding orientation silk fibroin hydrogel.
Above-mentioned stirring-head bottom shape and circular die shape match and guarantee stirring-head can in circular die water
Flat rotation, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
Mechanics Performance Testing is carried out using TMS-PRO Texture instrument to the hydrogel, the result is as follows:
3 compressive strength of table
| Compression direction | Compressive strength (KPa) | Standard error |
| Differently- oriented directivity | 204.1 | 18.45 |
| Non-oriented direction | 124 | 17.91 |
Table 4 cuts intensity
| Cut direction | It cuts intensity (KPa) | Standard error |
| Perpendicular to differently- oriented directivity | 39.62 | 5.22 |
| It is parallel to differently- oriented directivity | 25.31 | 3.13 |
Example three:
1,80 grams of silkworm raw silks are weighed, to 4L mass concentration be 0.25g/L sodium carbonate and mass concentration is 0.75g/L carbonic acid
After hydrogen sodium mixed aqueous solution boils, domestic silkworm silk is put into the solution boiled, the slightly boiled processing under the conditions of 98 DEG C~100 DEG C
It after 30min, is washed with deionized, is repeated twice aforesaid operations, make natural silk degumming, after finally being cleaned with deionized water, drying
60 DEG C of drying, can be obtained pure silk cellulose fiber in case；
2, the lithium-bromide solution of 9.3mol/L is used to dissolve with the bath raio of 1:10 pure silk cellulose fiber, in constant temperature magnetic force
It is cooled to room temperature after stirring and dissolving 30min at 65 ± 2 DEG C in blender to get fibroin albumen mixed solution is arrived；
3, by fibroin albumen mixed solution after cooling be fitted into molecular cut off be 8-10kDa bag filter in, spend from
After sub- water is dialysed 3 days, is filtered through absorbent cotton, pure silk fibroin protein solution can be obtained；
4, the concentration for adjusting silk fibroin protein solution is 51g/L, i.e. component A is deposited at a temperature of 4 DEG C；
5, compound concentration is the N- sodium lauroyl sarcosine solution of 80g/L, i.e. component B is deposited at a temperature of 4 DEG C；
6, above-mentioned component A and component B are uniformly mixed with the volume ratio of 10:1, mixed liquor is poured into circular die, is stirred
Mixing the stirring rod on device and connecting bottom surface is circular stirring-head, is heated with thermostat water bath, makes temperature of the mixed solution at 37 DEG C
And 57S-1Shear rate under shear 20min, obtain molding orientation silk fibroin hydrogel.
Above-mentioned stirring-head bottom shape and circular die shape match and guarantee stirring-head can in circular die water
Flat rotation, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
Mechanics Performance Testing is carried out using TMS-PRO Texture instrument to the hydrogel, the result is as follows:
5 compressive strength of table
| Compression direction | Compressive strength (KPa) | Standard deviation |
| Differently- oriented directivity | 224.0 | 20.46 |
| Non-oriented direction | 102.5 | 17.46 |
Table 6 cuts intensity
| Cut direction | It cuts intensity (KPa) | Standard deviation |
| Perpendicular to differently- oriented directivity | 52.49 | 7.36 |
| It is parallel to differently- oriented directivity | 39.59 | 4.82 |
Example four:
1,80 grams of silkworm raw silks are weighed, to 4L mass concentration be 0.25g/L sodium carbonate and mass concentration is 0.75g/L carbonic acid
After hydrogen sodium mixed aqueous solution boils, domestic silkworm silk is put into the solution boiled, the slightly boiled processing under the conditions of 98 DEG C~100 DEG C
It after 30min, is washed with deionized, is repeated twice aforesaid operations, make natural silk degumming, after finally being cleaned with deionized water, drying
60 DEG C of drying, can be obtained pure silk cellulose fiber in case；
2, the lithium-bromide solution of 9.3mol/L is used to dissolve with the bath raio of 1:10 pure silk cellulose fiber, in constant temperature magnetic force
It is cooled to room temperature after stirring and dissolving 30min at 65 ± 2 DEG C in blender to get fibroin albumen mixed solution is arrived；
3, by fibroin albumen mixed solution after cooling be fitted into molecular cut off be 8-10kDa bag filter in, spend from
After sub- water is dialysed 3 days, is filtered through absorbent cotton, pure silk fibroin protein solution can be obtained；
4, the concentration for adjusting silk fibroin protein solution is 11g/L, i.e. component A is deposited at a temperature of 4 DEG C；
5, compound concentration is the withered grass bacterium ester peptide sodium solution of 35g/L, i.e. component B is deposited at a temperature of 4 DEG C；
6, above-mentioned component A and component B are uniformly mixed with the volume ratio of 10:1, mixed liquor is poured into circular die, is stirred
Mixing the stirring rod on device and connecting bottom surface is circular stirring-head, is heated with thermostat water bath, makes temperature of the mixed solution at 37 DEG C
And 20S-1Shear rate under shear about 120min, obtain molding orientation silk fibroin hydrogel.
Above-mentioned stirring-head bottom shape and circular die shape match and guarantee stirring-head can in circular die water
Flat rotation, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
Mechanics Performance Testing is carried out using TMS-PRO Texture instrument to the hydrogel, the result is as follows:
7 compressive strength of table
| Compression direction | Compressive strength (KPa) | Standard deviation |
| Differently- oriented directivity | 50.96 | 19.45 |
| Non-oriented direction | 25.48 | 17.46 |
Table 8 cuts intensity
| Cut direction | It cuts intensity (KPa) | Standard deviation |
| Perpendicular to differently- oriented directivity | 12.56 | 9.35 |
| It is parallel to differently- oriented directivity | 10.43 | 10.2 |
Example five:
1,80 grams of silkworm raw silks are weighed, to 4L mass concentration be 0.25g/L sodium carbonate and mass concentration is 0.75g/L carbonic acid
After hydrogen sodium mixed aqueous solution boils, domestic silkworm silk is put into the solution boiled, the slightly boiled processing under the conditions of 98 DEG C~100 DEG C
It after 30min, is washed with deionized, is repeated twice aforesaid operations, make natural silk degumming, after finally being cleaned with deionized water, drying
60 DEG C of drying, can be obtained pure silk cellulose fiber in case；
2, the lithium-bromide solution of 9.3mol/L is used to dissolve with the bath raio of 1:10 pure silk cellulose fiber, in constant temperature magnetic force
It is cooled to room temperature after stirring and dissolving 30min at 65 ± 2 DEG C in blender to get fibroin albumen mixed solution is arrived；
3, by fibroin albumen mixed solution after cooling be fitted into molecular cut off be 8-10kDa bag filter in, spend from
After sub- water is dialysed 3 days, is filtered through absorbent cotton, pure silk fibroin protein solution can be obtained；
4, the concentration for adjusting silk fibroin protein solution is 55g/L, i.e. component A is deposited at a temperature of 4 DEG C；
5, compound concentration is the withered grass bacterium ester peptide sodium solution of 55g/L, i.e. component B is deposited at a temperature of 4 DEG C；
6, above-mentioned component A and component B are uniformly mixed with the volume ratio of 10:1, mixed liquor is poured into circular die, is stirred
Mixing the stirring rod on device and connecting bottom surface is circular stirring-head, is heated with thermostat water bath, makes temperature of the mixed solution at 45 DEG C
And 850S-1Shear rate under shear about 5min, obtain molding orientation silk fibroin hydrogel.
Above-mentioned stirring-head bottom shape and circular die shape match and guarantee stirring-head can in circular die water
Flat rotation, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
Mechanics Performance Testing is carried out using TMS-PRO Texture instrument to the hydrogel, the result is as follows:
9 compressive strength of table
| Compression direction | Compressive strength (KPa) | Standard deviation |
| Differently- oriented directivity | 389.6 | 18.64 |
| Non-oriented direction | 165.4 | 15.46 |
Table 10 cuts intensity
| Cut direction | It cuts intensity (KPa) | Standard deviation |
| Perpendicular to differently- oriented directivity | 96.69 | 10.3 |
| It is parallel to differently- oriented directivity | 45.54 | 8.62 |
Example six:
1,80 grams of silkworm raw silks are weighed, to 4L mass concentration be 0.25g/L sodium carbonate and mass concentration is 0.75g/L carbonic acid
After hydrogen sodium mixed aqueous solution boils, domestic silkworm silk is put into the solution boiled, the slightly boiled processing under the conditions of 98 DEG C~100 DEG C
It after 30min, is washed with deionized, is repeated twice aforesaid operations, make natural silk degumming, after finally being cleaned with deionized water, drying
60 DEG C of drying, can be obtained pure silk cellulose fiber in case；
2, the lithium-bromide solution of 9.3mol/L is used to dissolve with the bath raio of 1:10 pure silk cellulose fiber, in constant temperature magnetic force
It is cooled to room temperature after stirring and dissolving 30min at 65 ± 2 DEG C in blender to get fibroin albumen mixed solution is arrived；
3, by fibroin albumen mixed solution after cooling be fitted into molecular cut off be 8-10kDa bag filter in, spend from
After sub- water is dialysed 3 days, is filtered through absorbent cotton, pure silk fibroin protein solution can be obtained；
4, the concentration for adjusting silk fibroin protein solution is 35g/L, i.e. component A is deposited at a temperature of 4 DEG C；
5, compound concentration is the withered grass bacterium ester peptide sodium solution of 44g/L, i.e. component B is deposited at a temperature of 4 DEG C；
6, above-mentioned component A and component B are uniformly mixed with the volume ratio of 10:1, mixed liquor is poured into circular die, is stirred
Mixing the stirring rod on device and connecting bottom surface is circular stirring-head, is heated with thermostat water bath, makes temperature of the mixed solution at 10 DEG C
And 100S-1Shear rate under shear about 75min, obtain molding orientation silk fibroin hydrogel.
Above-mentioned stirring-head bottom shape and circular die shape match and guarantee stirring-head can in circular die water
Flat rotation, the bottom surface of the stirring-head is in contact with the mixed liquor liquid level in mold.
Mechanics Performance Testing is carried out using TMS-PRO Texture instrument to the hydrogel, the result is as follows:
11 compressive strength of table
Table 12 cuts intensity
| Cut direction | It cuts intensity (KPa) | Standard deviation |
| Perpendicular to differently- oriented directivity | 21.23 | 11.4 |
| It is parallel to differently- oriented directivity | 11.44 | 10.61 |
The above is only a preferred embodiment of the present invention, it is not intended to restrict the invention, it is noted that for this skill
For the those of ordinary skill in art field, without departing from the technical principles of the invention, can also make it is several improvement and
Modification, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (5)
1. a kind of preparation method for being orientated silk fibroin hydrogel, domestic silkworm silk obtain fibroin albumen water after degumming, dissolution, dialysis
Solution, which is characterized in that further include following steps:
(1) silk fibroin water solution that concentration is 10g/L~60g/L and the surface that concentration is 35g/L~100g/L is respectively configured
Aqueous surfactant solutions, then by silk fibroin water solution and aqueous surfactant solution respectively under the conditions of 2 DEG C -8 DEG C of temperature
Storage；
(2) ratio that silk fibroin water solution and aqueous surfactant solution are 10:1 by volume is mixed
Mixed liquor is poured into circular die and is sheared by liquid, and it is discoid stirring that bottom surface is connected in the stirring rod on blender
Head, heated at constant temperature make temperature and 20S of the mixed solution at 10 DEG C~50 DEG C-1~1000S-1Shear rate under shear 5min~
120min obtains orientation silk fibroin hydrogel；
The inner surface configuration of the stirring-head bottom shape and circular die matches and the stirring-head can be in circular die
Interior horizontal rotation, the bottom surface of the stirring-head are in contact with the mixed liquor liquid level in mold.
2. a kind of preparation method for being orientated silk fibroin hydrogel according to claim 1, it is characterised in that: the surface
Activating agent is anionic surfactant or nonionic surfactant.
3. it is according to claim 2 it is a kind of be orientated silk fibroin hydrogel preparation method, it is characterised in that: it is described yin from
Sub- surfactant are as follows: sodium bacillus subtilis lipopeptide, N- lauroyl musculamine acid sodium, odium stearate, potassium stearate, magnesium stearate, tristearin
One of acyl lactylate, stearyl lactate, casein sodium, aliphatic alcohol polyethenoxy sodium sulphate are several.
4. it is according to claim 2 it is a kind of be orientated silk fibroin hydrogel preparation method, it is characterised in that: it is described it is non-from
Sub- surfactant are as follows: sorbitan monostearate, sorbitol anhydride tristearate, sorbitan mono-oleic acid ester, sorbierite
One of acid anhydride monopalmitate, sucrose fatty ester, polysorbate, propylene glycol alginate are several.
5. a kind of orientation silk fibroin hydrogel, it is characterised in that: using the preparation of any one of claim 1-4 the method
At.
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