CN106139227A - A kind of antibacterial absorbable cellulose dressing containing nanometer gold - Google Patents
A kind of antibacterial absorbable cellulose dressing containing nanometer gold Download PDFInfo
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- CN106139227A CN106139227A CN201610578972.8A CN201610578972A CN106139227A CN 106139227 A CN106139227 A CN 106139227A CN 201610578972 A CN201610578972 A CN 201610578972A CN 106139227 A CN106139227 A CN 106139227A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/02—Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/009—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The present invention provides a kind of antibacterial absorbable cellulose dressing containing nanometer gold, the described antibacterial absorbable cellulose dressing containing nanometer gold to be the absorbable cellulose dressing containing gold nano grain being attached with and having antibacterial action.This antibacterial absorbable cellulose dressing has the broad spectrum antibacterial of excellence, without bacterial drug resistance, the antimicrobial nano gold little to human body cell toxicity is added in absorbable cellulose dressing, make it have liquid suction function, moisture-keeping functions, hemostatic function, antibacterial functions and little to human body cell toxicity, can be used for clinical treatment of wounds, operation prevents organ adhesion, improves looks or treat burn, the various traumatic infections such as scald, decubital ulcer infection, are the most excellent a kind of medical dressing.Present invention also offers the preparation method and application of this antibacterial absorbable cellulose dressing.
Description
Technical field
The present invention relates to a kind of antibacterial absorbable cellulose dressing, particularly relate to a kind of antibacterial absorbable fibre containing nanometer gold
Dimension element dressing, and the preparation method and application of the described antibacterial absorbable cellulose dressing containing nanometer gold.
Background technology
When processing patient's wound, need to cover dressing on wound, prevent antibacterial from invading and preventing moisture loss.Tradition
Though effect protected to wound surface such as dressing such as gauze, binder etc., but moisturizing imbibition ability is poor, can only absorb the 4-5 of own wt
Times, and for some wounds when removing traditional dressing, dressing easy adhesion wound tissue, causes secondary wound to destroy；?
In surgical operation, the most usually need some degradables, the dressing of good biocompatibility is adhered to place organ.
Absorbable cellulose dressing, as renewable oxidized cellulose, hydroxyethyl cellulose dressing can overcome traditional dressing
Deficiency, can quickly absorb the moisture in blood and dissolve when absorbable cellulose dressing runs into blood, and the colloid of formation can block
Blood capillary end, reaches the purpose of hemostasis.Owing to it has good histocompatibility and hemostatic, can absorb in vivo,
Now it is widely used in surgical wound surface hemostasis and various treatment of wounds.Absorbable cellulose dressing in the market is a lot
Carboxyl or carboxymethyl cellulose product, belong to ionic cellulose element derivant, molecule slant acidity, this kind of absorbable cellulose dressing
By a definite limitation on drug carrying ability, its acidity can cause certain neurotoxicity for some wounds, and fine with ethoxy
Dimension element be main absorbable cellulose dressing owing to being nonionic cellulose derivatives, molecule is neutrality, compared to ion-type fibre
Dimension element dressing, the absorbable cellulose dressing based on hydroxyethyl cellulose has more preferably performance.
In absorbable cellulose dressing, add the antibacterial antibiotic property by this dressing of raising, more improve its function, and
And transport, preservation of carrying and sterilize can be made it easier to.Silver has a stronger anti-microbial property, but due to silver unstability and
Relatively big to the toxicity of human body cell, U.S. FDA the most clearly forbids silver use in terms of medical treatment, China CFDA medical some
Aspect limits the use of argentiferous curable product.
In recent years, stability is more preferably, gradually reported as the research of antibacterial the less nanometer gold of human body cell toxicity
Road.(Zhao Y, Tian Y, Cui Y, the et al.Small molecule-capped gold such as Zhao yuyun
nanoparticles as potent antibacterial agents that target gram-negative
bacteria[J].Journal of the American Chemical Society,2010,132(35):12349-
12356；Zhao Y,Chen Z,Chen Y,et al.Synergy of non-antibiotic drugs and
pyrimidinethiol on gold nanoparticles against superbugs[J].Journal of the
American Chemical Society, 2013,135 (35): 12940-12943. and Zhao Y, Ye C, Liu W, et
al.Tuning the composition of AuPt bimetallic nanoparticles for antibacterial
application[J].Angewandte Chemie International Edition,2014,53(31):8127-
8131.) the nanometer gold antibacterial of the double-component drug modification of the aminopyrimidine reported or aminopyrimidine/containing the little molecule of amino and receiving
Rice gold platinum bimetallic antibacterial has the strongest broad spectrum antibacterial, has the strongest killing action especially to Multidrug resistant bacteria, and it is to people
Somatic toxicity is well below nanometer silver, and will not produce bacterial drug resistance, is the most promising antibacterial of class.
Anna Regiel-Futyra(Regiel-Futyra A,M,Sebastian V,et
al.Development of Noncytotoxic Chitosan–Gold Nanocomposites as Efficient
Antibacterial Materials[J].ACS applied materials&interfaces,2015,7(2):1087-
1099.) nanometer gold of the chitosan complex reported also has the antibiotic property of excellence and the toxicity low to human body cell.
The nanometer gold of above-mentioned report synthesis needs dialysis treatment, and technique is more complicated, and technique needs to be simplified further；Simple
Nano-Au solution is not readily used for Wound healing and bone regeneration and the reparation of clinic, and anti-microbial property needs to be improved further.
At present, there is no what the nanometer gold that open source literature report is added with broad spectrum antibacterial and cytotoxicity is little made both at home and abroad
Absorbable cellulose dressing.
Summary of the invention
Therefore, it is an object of the invention to provide a kind of have broad spectrum antibacterial and to human body cell toxicity little containing nanometer
The antibacterial absorbable cellulose dressing of gold, additionally provides the preparation method of the described antibacterial absorbable cellulose dressing containing nanometer gold
And application.
To achieve these goals, the invention provides a kind of antibacterial absorbable cellulose dressing containing nanometer gold, described
Antibacterial absorbable cellulose dressing containing nanometer gold is the absorbable fibre containing gold nano grain being attached with and having antibacterial action
Element dressing.
The antibacterial absorbable cellulose dressing containing nanometer gold provided according to the present invention, wherein, described containing gold nano grain
Account for 0.01～3 mass % (i.e. mass percent) of described absorbable cellulose dressing.Preferably, described containing gold nano grain
Mean diameter is at below 50nm.More preferably 2～10nm.
According to the antibacterial absorbable cellulose dressing containing nanometer gold of present invention offer, wherein, described in, there is anti-microbial property
Containing gold nano grain selected from nanometer gold, the nanometer gold modified by one-component or multicomponent small-molecule drug or macromole and
One or more in double-component containing nanometer gold or multicomponent metal nanoparticle.
Preferably, the described nanometer gold modified by one-component or multicomponent small-molecule drug or macromole is with phonetic containing amino
Nanogold particle that pyridine micromolecular is modified as one-component or using containing aminopyrimidine micromolecular/amino micromolecular as double
The nanogold particle that component is modified, the described molar content containing aminopyrimidine micromolecular and gold element ratio for 0.5:1～2:1,
The molar content of described amino micromolecular and gold element is than for 0.5:1～2:1, most preferably, described little containing Aminopyrimidines
Molecule is 4,6-2 amino-2-mercapto phenyl formic pyrimidine, and amino micromolecular is metformin or 1-(3-chlorophenol) double arcs.
Or preferably, described double-component containing nanometer gold or multicomponent metal nanoparticle are that nanometer gold-platinum bimetallic is received
Rice grain；Most preferably, described nanometer gold-platinum duplex metal nano granule is by the gold of 80% atomic percent and 20% atomic percent
The platinum composition of ratio.
The antibacterial absorbable cellulose dressing containing nanometer gold provided according to the present invention, wherein, described absorbable cellulose
Dressing comprises hydroxyethyl cellulose and/or oxidized cellulose.Such as, the absorbable cellulose dressing in the present invention can be with hydroxyl
Ethyl cellulose is main cellulose dressing.Preferably, described absorbable cellulose dressing also comprises other absorbable celluloses.
It is highly preferred that other absorbable celluloses described are carboxymethyl cellulose.
Present invention also offers the preparation method of a kind of above-mentioned antibacterial absorbable cellulose dressing containing nanometer gold, wherein,
Described preparation method comprises the following steps:
(1) nano-Au solution is stirred with absorbable fibre cellulose solution or coagulant liquid, stand froth breaking acquisition mixing molten
Liquid or mixed gel liquid；Described nano-Au solution be containing described in there is the dispersion liquid containing gold nano grain of antibacterial action；
(2) the described mixed solution in step (1) or mixed gel liquid are laid in film formation device, put into baking oven or freezing
Drying machine is dried, prepares the described antibacterial absorbable cellulose dressing containing nanometer gold.Described antibacterial absorbable containing nanometer gold
The operations such as cellulose dressing can also carry out cutting, packs, sterilizing are with on-demand preparation casting product.
Preferably, the described dry temperature in step (2) is less than 200 DEG C.It is highly preferred that the institute in described step (2)
The baking temperature stating baking oven is 150～160 DEG C, and drying time is 6～10min.The baking temperature of described lyophilization machine is-40
～0 DEG C, described drying time is that 24h maybe can also be for a few hours.
According to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold that the present invention provides, wherein, step (1)
Described in nano-Au solution concentration be 100ppm～4000ppm；Described solidifying at described mixed solution or mixing containing gold nano grain
In glue, concentration is 5ppm～300ppm.Wherein, 1ppm generally can be scaled 1mg/L.
According to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold that the present invention provides, wherein, step (1)
Described in absorbable cellulose mass percent in described mixed solution or mixed gel liquid be 0.5%～10%.
According to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold that the present invention provides, wherein, step (1)
In, described mixing time is 20min～2h.Preferably, described time of repose is 1h～5h.
According to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold that the present invention provides, wherein, step (1)
Described in nano-Au solution through the following steps that prepare:
(a) according to proportioning, by chlorauric acid solution with containing aminopyrimidine micromolecular and/or the solution of amino micromolecular or
Platinum acid chloride solution mixes, and obtains mixed solution；
B () under agitation rapidly joins reducing agent in described mixed solution, fully i.e. obtain described nanometer gold after reaction
Solution；
Wherein, the joining day of described reducing agent be less than 5 minutes, preferably more than 1 minute, more preferably 10～15 seconds.
Preferably, the solution containing aminopyrimidine micromolecular and/or amino micromolecular described in step (a) can also wrap
Include organic acid or mineral acid.It is preferably acetic acid, propanoic acid or hydrochloric acid.It is highly preferred that the percent mass that acid is in described mixed solution
Ratio is 0.01%～10%.
Preferably, contain in the solution of aminopyrimidine micromolecular and/or amino micromolecular all right described in step (a)
Including nonionic surfactant.It is preferably tween or Polyethylene Glycol.It is highly preferred that nonionic surfactant is in described mixing
Mass percent in solution is 0.005%～10%.
Preferably, reducing agent described in step (b) can be selected from sodium borohydride, sodium citrate and sodium ascorbate
Plant or multiple.Described in step (b), abundant reaction temperature can be below room temperature or room temperature, and for example, less than 25 DEG C, the time can
Think 0.5～3 hour.
Preferably, the rubbing of described reducing agent and the mol ratio of gold chloride or described reducing agent and gold chloride and potassium chloroplatinate
That ratio is 1:1～10:1.More preferably 2:1～3:1.
It is highly preferred that the preparation method of described nano-Au solution does not comprise the step of nano-Au solution described in purification.Enter one
Preferably, the step of nano-Au solution described in purification includes processing with bag filter dialysis treatment or centrifugal filtration step.In other words, originally
Invention production method need not carry out purified nanotubes gold solution by modes such as bag filter dialysis treatment or centrifugal filtration process.As more
Further limiting, the preparation method of above-mentioned nano-Au solution can also be expressed as being made up of step (a) and step (b).
It should be readily apparent to one skilled in the art that the gold chloride raw material used when preparing chlorauric acid solution can be four hydrations
Gold chloride.
Present invention also offers the above-mentioned antibacterial absorbable cellulose dressing containing nanometer gold or use above-mentioned preparation method
The antibacterial absorbable cellulose dressing containing nanometer gold of preparation prevents organ from gluing when preparation is for clinical treatment of wounds, operation
Even, improve looks or treat the application in burn, scald, the medicine of decubital ulcer infection or product.
Compared with existing carboxyl or carboxymethyl cellulose dressing, the antibacterial absorbable fibre containing nanometer gold that the present invention prepares
Element dressing is nonionic cellulose dressing, is therefore not limited on drug carrying ability, and molecule is neutrality, and it will not be to wound
Mouth causes neurotoxicity.
The antibacterial absorbable cellulose dressing containing nanometer gold that the present invention prepares has the broad spectrum antibacterial of excellence, without antibacterial
Drug resistance, adds to the antimicrobial nano gold little to human body cell toxicity in absorbable cellulose dressing so that it is have imbibition merit
Energy, moisture-keeping functions, hemostatic function, antibacterial functions and little to human body cell toxicity, can be used for clinical treatment of wounds, preventer of performing the operation
Official's adhesion, beauty treatment, treat the various traumatic infections such as burn, scald, decubital ulcer infection, be the most excellent a kind of medical dressing.
In the preparation method of the antimicrobial nano gold reported in prior art, reducing agent is to add in the way of dropping, right
In industrialized production, the dropping mode of background report is the most oversize, it is impossible to realize, and the nano-Au solution provided in the present invention
In preparation method, reducing agent, for be disposably added rapidly to described mixed solution, the most more saves the time, contributes to promoting production
Efficiency.
The nano-Au solution of background report needs with bag filter dialysis or centrifugation after completion of the reaction, for industrialization
Producing, the post-processing approach of background report is complicated, apparatus expensive.And the present invention provide nano-Au solution preparation method not
Needing dialysis or centrifugation to process, therefore technique is the simplest, reduces production cost, improves production efficiency further.
Being proved by testing inspection, the antibacterial effect of the nano-Au solution prepared by preparation method that the present invention provides is the most excellent
In reducing agent dropping and/or through the nano-Au solution prepared by dialysis treatment.The present invention provides receiving prepared by preparation method
The cytotoxicity of rice gold solution is significantly lower than nanometer silver solution and silver nitrate solution.
Accompanying drawing explanation
Hereinafter, describe embodiment of the present invention in detail in conjunction with accompanying drawing, wherein:
Fig. 1 shows embodiment 2 nanometer gold and is purchased nanometer silver transmission electron microscope (TEM) figure；
Fig. 2 show embodiment 2 nano-Au solution, be purchased nanometer silver, the cytotoxicity that is purchased silver nitrate compares.
Detailed description of the invention
The present invention is further illustrated below by specific embodiment, it should be understood, however, that, these embodiments are only
It is used for specifically describing in more detail, and is not to be construed as limiting in any form the present invention.
This part to the present invention test used in material and test method carry out general description.Although it is
Realize many materials that the object of the invention used and operational approach is to it is known in the art that but the present invention still uses up at this
May describe in detail.It will be apparent to those skilled in the art that within a context, if not specified, material therefor of the present invention and behaviour
It is well known in the art as method.
The reagent and the instrument that use in following example are as follows:
Instrument:
Inductively coupled plasma atomic emission spectrometer (ICP-OES)；Model iCAP 6300；It is purchased from: U.S. Thermo
Scientific company
Transmission electron microscope (TEM)；Model Tecnai G2 20S-TWIN；It is purchased from: FEI Co. of the U.S.
Embodiment 1
The present embodiment is for antibacterial absorbable cellulose dressing containing nanometer gold that the present invention is described and preparation method thereof.
(1) be 10ml using volume, concentration be 800ppm 4,6-2 amino-2-mercapto phenyl formic pyrimidine/1-(3-chlorophenol) biguanide as
The small numerator modified nano-Au solution of double-component is 300ml with volume, hydroxyethyl cellulose solution containing 0.4g stirs, and stirs
The time of mixing is 50mim, stands froth breaking, and time of repose is 2h, it is thus achieved that mixed solution, nanometer gold concentration in described mixed solution
For 5ppm, hydroxyethyl cellulose mass percent in described mixed solution is 0.5%；
(2) the described mixed solution in step (1) being laid in film formation device, put into oven drying, baking temperature is 150
DEG C, it is dried 10 minutes and prepares the described antibacterial absorbable cellulose dressing containing nanometer gold.
(3) the described antibacterial absorbable cellulose dressing containing nanometer gold cut, pack, sterilizing.
The preparation side of the nanometer gold that above-mentioned 4,6-2 amino-2-mercapto phenyl formic pyrimidine/1-(3-chlorophenol) biguanide is modified as double-component
Method is:
A the four hydration gold chlorides of 0.04g are dissolved in the deionized water of 0.01L by (), obtain chlorauric acid solution.By a certain amount of
4,6-2 amino-2-mercapto phenyl formic pyrimidine and 1-(3-chlorophenol) biguanide are dissolved in the deionized water of 0.01L, are additionally added a certain amount of telling
Temperature 80 and acetic acid, is completely dissolved in water with amino micromolecular helping lend some impetus to aminopyrimidine micromolecular, obtains containing ammonia
Yl pyrimidines micromolecular and/or the solution of amino micromolecular.By two solution mixing, obtain mixed solution.
B a certain amount of sodium ascorbate is dissolved in the deionized water of 0.005L by (), under agitation by above-mentioned ascorbic acid
Sodium solution rapidly join (used time 10s) in above-mentioned mixed solution, 1 hour stirred below of room temperature to fully reaction, be not required to adopt
With purifying process such as dialysis, i.e. obtain the concentration of about 0.025L be about (the calculating according to rate of charge) of 800ppm containing above-mentioned 4,
The nano-Au solution of the nanogold particle that 6-2 amino-2-mercapto phenyl formic pyrimidine/1-(3-chlorophenol) biguanide is modified as double-component.
According to rate of charge, 4,6-2 amino-2-mercapto phenyl formic pyrimidines are more double than for 2:1,1-(3-chlorophenol) with the molar content of gold element
The molar content of guanidine and gold element is than for 0.5:1, and the molar content of reducing agent sodium ascorbate and gold chloride is than for 3:1, acetic acid
The mass percent accounting for nano-Au solution is 0.01%, and surface active agent tween 80 accounts for the mass percent of nano-Au solution and is
0.01%.
In this antibacterial absorbable cellulose dressing containing nanometer gold, measuring through ICP-OES, every absorbable cellulose applies
The gold content of material is 0.1% (mass percent).Through 200KV lanthanum hexaboride determination of transmission electron microscopy, containing in this dressing
The mean diameter of gold nano grain is 6nm.
Comparative example 1
Prepare the nanometer gold antiseptic solution of comparative example 1 according to preparation method same as in Example 1, add a certain amount of going
Ionized water, is configured to the nanometer gold antiseptic solution of variable concentrations, wherein, including with the nanometer gold absorbable fibre in embodiment 1
Element dressing has the nano-Au solution of identical nanometer gold weight/mass percentage composition.
Comparative example 1.1
According to the nanometer gold antiseptic solution of the comparative example 1.1 that the preparation method similar to comparative example 1 prepares, comparative example 1.1
Middle reductant solution joins in reaction solution in the way of dropping, and uses the dialysis treatment mode of 24 hours to obtain nanometer
Gold antiseptic solution, adds purified water so that it is have identical nanometer gold percent mass to contain with the nanometer gold antiseptic solution in comparative example 1
Amount.
Test example 1
By the antibacterial experiment in test example 1, it was demonstrated that the nanometer gold antiseptic solution of comparative example 1 is better than comparative example 1.1
Nanometer gold antiseptic solution.Antibacterial effect is shown in Table 1.
Comparative approach is as follows: uses minimal inhibitory concentration MIC (mg/L) to weigh, uses micropore dilution process
(National Committee for Clinical Laboratory Standards., Methodsfor determining
Bactericidal activity of antimicrobial agents, M26-A, 1999.), by 100 μ L variable concentrations
The nano-Au solution of comparative example 1 and comparative example 1.1 is added separately in 96 microwell plates, 10 μ L same concentrations (104CFU/mL)
Inoculum adds in each micropore, 37 DEG C cultivate 24 hours after observe, in the micropore of the bacterial growth that not can be seen that
Few concentration is minimal inhibitory concentration (MIC).
Test result is shown in Table 1.From test result, the antibacterial effect of the nanometer gold antiseptic solution of comparative example 1 is much better than
The antibacterial effect of the nanometer gold antiseptic solution of comparative example 1.1.Illustrate to use the nano-Au solution identical with comparative example 1 preparation method
The antibacterial absorbable cellulose dressing containing nanometer gold of preparation, its antibacterial effect also should be much better than employing to be prepared with comparative example 1.1
Antibacterial absorbable cellulose dressing containing nanometer gold prepared by the nano-Au solution that method is identical.
The anti-microbial property of the nanometer gold antiseptic solution of table 1 comparative example 1 and comparative example 1.1 compares
Embodiment 2
The present embodiment is for antibacterial absorbable cellulose dressing containing nanometer gold that the present invention is described and preparation method thereof.
(1) be 10ml using volume, concentration be that the 4,6-2 amino-2-mercapto phenyl formic pyrimidine/metformin of 100ppm is as double-component
Small numerator modified nano-Au solution and volume be 90ml, coagulant liquid stirring containing 10g hydroxyethyl cellulose and oxidized cellulose
Uniformly, mixing time is 20min, stands froth breaking, and time of repose is 1h, it is thus achieved that mixed gel liquid, and nanometer gold coagulates in described mixing
Concentration in glue is 10ppm, and hydroxyethyl cellulose mass percent in described mixed gel liquid is 7%, oxidized fibre
Element mass percent in described mixed gel liquid is 3%；
(2) the described mixed gel liquid in step (1) is laid in film formation device, puts into freezer dryer and be dried, be dried
Temperature is-20 DEG C, is dried 24h, prepares the described antibacterial absorbable cellulose dressing containing nanometer gold.
(3) the described antibacterial absorbable cellulose dressing containing nanometer gold cut, pack, sterilizing.
The preparation method of the nanometer gold that above-mentioned 4,6-2 amino-2-mercapto phenyl formic pyrimidine/metformin is modified as double-component is:
A the four hydration gold chlorides of 0.005g are dissolved in the deionized water of 0.01L by (), obtain chlorauric acid solution.Will be certain
The 4 of amount, 6-2 amino-2-mercapto phenyl formic pyrimidine and metformin are dissolved in the deionized water of 0.01L, are additionally added a certain amount of Tween 80
And acetic acid, it is completely dissolved in water helping lend some impetus to aminopyrimidine micromolecular and amino micromolecular, obtains containing amino phonetic
Pyridine micromolecular and/or the solution of amino micromolecular.By two solution mixing, obtain mixed solution.
B a certain amount of sodium ascorbate is dissolved in the deionized water of 0.005L by (), under agitation by above-mentioned ascorbic acid
Sodium solution rapidly join (used time 5s) in above-mentioned mixed solution, 1 hour stirred below of room temperature to fully reaction, be not required to use
The purifying process such as dialysis, the concentration i.e. obtaining about 0.025L is about (the calculating according to rate of charge) of 100ppm containing above-mentioned 4,6-
The nano-Au solution of the nanogold particle that 2 amino-2-mercapto phenyl formic pyrimidines/metformin is modified as double-component.
According to rate of charge, the molar content of 4,6-2 amino-2-mercapto phenyl formic pyrimidines and gold element ratio for 0.5:1, metformin with
The molar content of gold element is than for 2:1, and the molar content of reducing agent sodium ascorbate and gold chloride is than for 2:1, and acetic acid accounts for nanometer
The mass percent of gold solution is 0.01%, and it is 0.005% that surface active agent tween 80 accounts for the mass percent of nano-Au solution.
In this antibacterial absorbable cellulose dressing containing nanometer gold, measuring through ICP-OES, every absorbable cellulose applies
The gold content of material is 0.01% (mass percent).Through 200KV lanthanum hexaboride determination of transmission electron microscopy, containing in this dressing
The mean diameter of gold nano grain is 4nm.
Comparative example 2
Prepare the nanometer gold antiseptic solution of comparative example 2 according to preparation method same as in Example 2, add a certain amount of going
Ionized water, makes the nanometer gold antiseptic solution that nanometer gold mass percent is 0.01%, can absorb with the nanometer gold in embodiment 2
Dimension element dressing has identical nanometer gold weight/mass percentage composition.
Embodiment 3
The present embodiment is for antibacterial absorbable cellulose dressing containing nanometer gold that the present invention is described and preparation method thereof.
(1) by volume be 10ml, concentration be nanometer gold-platinum (duplex metal nano granule Au of 4000ppm80Pt20) solution
(i.e. nano-Au solution) and volume are 123ml, the oxidized cellulose coagulant liquid containing 4g stirs, and mixing time is 2h, and standing disappears
Bubble, time of repose is 5h, it is thus achieved that mixed solution, and nanometer gold-platinum concentration in described mixed solution is 300ppm, oxidized fibre
Element mass percent in described mixed solution is 3%；
(2) the described mixed solution in step (1) being laid in film formation device, put into oven drying, baking temperature is 160
DEG C, it is dried 6 minutes, prepares the described antibacterial absorbable cellulose dressing containing nanometer gold.
(3) the described antibacterial absorbable cellulose dressing containing nanometer gold cut, pack, sterilizing.
The preparation method of above-mentioned nanometer gold-platinum duplex metal nano granule is:
A () is by the four hydration gold chloride (HAuCl of 0.14g4·4H2And 0.04g potassium chloroplatinate (K O)2PtCl6) be dissolved in
In the deionized water of 0.02L, obtain mixed solution.
B a certain amount of sodium borohydride is dissolved in the deionized water of 0.005L by (), under agitation by molten for above-mentioned sodium borohydride
Liquid rapidly join (used time 15s) in above-mentioned mixed solution, 1 hour stirred below of room temperature to fully reaction, be not required to use thoroughly
The purifying process such as analysis, the concentration about 4000ppm i.e. obtaining about 0.025L contains the nanometer of nanometer gold-platinum duplex metal nano granule
Gold solution.
According to rate of charge, the molar content of borane reducing agent sodium hydride and gold chloride and potassium chloroplatinate is than for 3:1, the atom of platinum
Percentage ratio is 20%, and therefore this gold-Pt nanoparticle is represented by Au80Pt20Nano-particle.
In this antibacterial absorbable cellulose dressing containing nanometer gold, measuring through ICP-OES, every absorbable cellulose applies
Material is 3% (mass percent) containing gold-platinum amount.Through 200KV lanthanum hexaboride determination of transmission electron microscopy, containing in this dressing
The mean diameter of gold nano grain is 4nm.
Comparative example 3
Prepare the nanometer gold-platinum antiseptic solution of comparative example 3 according to preparation method same as in Example 3, add certain
Amount deionized water so that it is have identical nanometer gold-platinum percent mass with the nanometer gold in embodiment 3-platinum oxidation cellulose dressing
Content.
Test example 2
The nanometer gold that 4,6-2 amino-2-mercapto phenyl formic pyrimidine/metformin embodiment 2 used is modified as double-component resists
Bacterium solution, compared with the anti-microbial property of the antiseptic solution (purchased from Zhangjiagang Nai Er nanosecond science and technology company) being purchased containing nanometer silver, is said
The antibacterial absorbable cellulose dressing containing this nanometer gold of the bright present invention also should have good anti-microbial property.
Comparative approach is as follows: uses minimal inhibitory concentration MIC (mg/L) to weigh, uses micropore dilution process
(National Committee for Clinical Laboratory Standards., Methods for
Determining bactericidal activity of antimicrobial agents, M26-A, 1999.), by 100 μ
The nanometer gold of L variable concentrations or nanometer silver join in 96 microwell plates, 10 μ L same concentrations (104CFU/mL) inoculum
Add in each micropore, cultivate for 37 DEG C and observe after 24 hours, nanometer gold minimum in the micropore of the bacterial growth that not can be seen that
Or the concentration of nanometer silver is minimal inhibitory concentration (MIC).Test result is shown in Table 2.
From test result, the 4 of embodiment 2 employing, 6-2 amino-2-mercapto phenyl formic pyrimidine/metformin is repaiied as double-component
The escherichia coli of nanometer gold antiseptic solution of decorations and the MIC of bacillus pyocyaneus are both less than purchased the MIC of the antiseptic solution containing nanometer silver,
Thus illustrating, the anti-microbial property of escherichia coli and bacillus pyocyaneus is better than being purchased the antiseptic solution containing nanometer silver by embodiment 2.
The anti-microbial property comparative result of table 2 embodiment 2 nanometer gold antiseptic solution and the antiseptic solution being purchased containing nanometer silver
Test example 3
By the dressing Bactericidal test of different nanometer gold content, the antibacterial absorbable cellulose containing this nanometer gold is described
The bacteriostatic activity of dressing.
Test, to embodiment 1-3 and comparative example according to " medicine Micro biological Tests handbook " the 18th chapter Section 1 cup-plate method
1-3 carries out inhibition zone test.Result of the test is shown in Table 3.The inhibition zone of blank absorbable cellulose is 10mm, and inhibition zone size is can
Absorb the original size of cellulose sample, there is no fungistatic effect.
Test result is visible, although the nanometer gold mass percent of each embodiment is different with type, but all can be observed bright
Aobvious inhibition zone, illustrates that the absorbable cellulose dressing containing nanometer gold is to escherichia coli, staphylococcus aureus and bacillus pyocyaneus
All there is good fungistatic effect.And the inhibition zone of the same type of absorbable cellulose dressing containing nanometer gold of same concentration is the biggest
In nanometer gold antiseptic solution, illustrate that the fungistatic effect of the absorbable cellulose dressing containing nanometer gold has been better than identical nanometer gold and has contained
Measure the nanometer gold antiseptic solution of identical nanometer gold type.
The absorbable cellulose dressing of the different nanometer gold content of table 3 and solution inhibition zone test result (unit: mm)
Test example 4 cytotoxicity experiment
Cell toxicity test operating process: Human umbilical vein endothelial cells (HUVEC) is clear containing 10% N of fetal blood
The Eagle culture medium (DMEM) that Dulbecco improves is cultivated.10 are added in each hole of 96 microwell plates4Individual HUVEC cell,
Each hole is separately added into the not dialysis treatment containing variable concentrations, rapidly joins the 4,6-2 amino-2-mercapto phenyl formic that reducing agent is produced
Pyrimidine/metformin as the small numerator modified nano-Au solution of double-component, be purchased nanometer silver, to be purchased silver nitrate solution 200 micro-
Rise.Cultivate 48 hours in microporous medium 37 DEG C, do not add the HUVEC cell of antibacterial as comparison.After having cultivated,
Solution in micropore phosphate buffer solution (PBS, 0.01mol/L, pH 7.4) is washed once, and the CCK-8 adding 10% (v/v) is molten
Liquid, in microporous medium, is cultivated 2 hours at 37 DEG C.Various kinds sample wells and comparison is measured at enzyme-linked immunosorbent assay instrument 450nm
The light absorption value in hole.Calculate each sample well cell relative viability.Each sample porocyte relative activity is defined as: each hole extinction
Light absorption value × 100% of value/control wells.Result of the test is shown in Fig. 2.
Knowable to the result of the test shown in Fig. 2, the nano-Au solution that the present invention uses cytotoxicity under variable concentrations
All significantly lower than being purchased nanometer silver and being purchased silver nitrate solution.
Although present invention has been a certain degree of description, it will be apparent that, without departing from the spirit and scope of the present invention
Under the conditions of, the suitable change of each condition can be carried out.It is appreciated that and the invention is not restricted to described embodiment, and be attributed to right
The scope required, it includes the equivalent of described each factor.
Claims (10)
1. the antibacterial absorbable cellulose dressing containing nanometer gold, it is characterised in that described antibacterial absorbable containing nanometer gold
Cellulose dressing is the absorbable cellulose dressing containing gold nano grain being attached with and having antibacterial action.
Antibacterial absorbable cellulose dressing containing nanometer gold the most according to claim 1, it is characterised in that described containing Jenner
Rice grain accounts for 0.01～3 mass % of described absorbable cellulose dressing, it is preferable that the described mean diameter containing gold nano grain
At below 50nm；More preferably 2～10nm.
Antibacterial absorbable cellulose dressing containing nanometer gold the most according to claim 1 and 2, it is characterised in that described tool
Have anti-microbial property is selected from nanometer gold containing gold nano grain, by receiving that one-component or multicomponent small-molecule drug or macromole are modified
Meter Jin and containing one or more in the double-component of nanometer gold or multicomponent metal nanoparticle；
Preferably, the described nanometer gold modified by one-component or multicomponent small-molecule drug or macromole is with containing Aminopyrimidines
Nanogold particle that little molecule is modified as one-component or using containing aminopyrimidine micromolecular/amino micromolecular as double-component
The nanogold particle modified, the described molar content containing aminopyrimidine micromolecular and gold element ratio is for 0.5:1～2:1, described
The molar content of amino micromolecular and gold element is than for 0.5:1～2:1, most preferably, described containing aminopyrimidine micromolecular
Being 4,6-2 amino-2-mercapto phenyl formic pyrimidine, amino micromolecular is metformin or 1-(3-chlorophenol) double arcs；
Or preferably, described double-component containing nanometer gold or multicomponent metal nanoparticle are nanometer gold-platinum bimetal nano
Grain；Most preferably, described nanometer gold-platinum duplex metal nano granule is by the gold of 80% atomic percent and 20% atomic percent
Platinum forms.
Antibacterial absorbable cellulose dressing containing nanometer gold the most according to any one of claim 1 to 3, its feature exists
In, described absorbable cellulose dressing comprises hydroxyethyl cellulose and/or oxidized cellulose；Preferably, described absorbable fibre
Element dressing also comprises other absorbable celluloses；It is highly preferred that other absorbable celluloses described are carboxymethyl cellulose.
5. the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold according to any one of Claims 1-4, it is special
Levying and be, described preparation method comprises the following steps:
(1) nano-Au solution is stirred with absorbable fibre cellulose solution or coagulant liquid, stand froth breaking obtain mixed solution or
Mixed gel liquid；Described nano-Au solution be containing described in there is the dispersion liquid containing gold nano grain of antibacterial action；
(2) the described mixed solution in step (1) or mixed gel liquid are laid in film formation device, put into baking oven or lyophilization
Machine is dried, prepares the described antibacterial absorbable cellulose dressing containing nanometer gold；
Preferably, the described dry temperature in step (2) is less than 200 DEG C；It is highly preferred that the described baking in described step (2)
The baking temperature of case is 150～160 DEG C, and drying time is 6～10min；The baking temperature of described lyophilization machine be-40 DEG C～
0 DEG C, drying time is 24h.
The preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold the most according to claim 5, it is characterised in that
Described in step (1), nano-Au solution concentration is 100ppm～4000ppm；Described containing gold nano grain at described mixed solution or
In mixed gel liquid, concentration is 5ppm～300ppm.
7., according to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold described in claim 5 or 6, its feature exists
In, the mass percent in described mixed solution or mixed gel liquid of absorbable cellulose described in step (1) be 0.5%～
10%.
8. according to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold according to any one of claim 5 to 7,
It is characterized in that, in step (1), described mixing time is 20min～2h；Preferably, described time of repose is 1h～5h.
9. according to the preparation method of the antibacterial absorbable cellulose dressing containing nanometer gold according to any one of claim 5 to 8,
It is characterized in that, nano-Au solution described in step (1) is through the following steps that prepare:
(a) according to proportioning, by chlorauric acid solution with containing aminopyrimidine micromolecular and/or the solution of amino micromolecular or chlorine platinum
Acid solution mixes, and obtains mixed solution；
B () under agitation rapidly joins reducing agent in described mixed solution, fully both obtained described nano-Au solution after reaction；
Wherein, the joining day of described reducing agent be less than 5 minutes, preferably more than 1 minute, more preferably 10～15 seconds；
Preferably, the mol ratio of described reducing agent and gold chloride or described reducing agent and gold chloride and the mol ratio of potassium chloroplatinate
For 1:1～10:1, more preferably 2:1～3:1；
It is highly preferred that the preparation method of described nano-Au solution does not comprise the step of nano-Au solution described in purification；The most excellent
Selection of land, the step of nano-Au solution described in purification includes processing with bag filter dialysis treatment or centrifugal filtration.
10. the antibacterial absorbable cellulose dressing containing nanometer gold according to any one of Claims 1-4 or employing claim
Antibacterial absorbable cellulose dressing containing nanometer gold prepared by preparation method according to any one of 5 to 9 is hindered for clinic in preparation
Prevent organ adhesion when mouth process, operation, improve looks or treat the application in burn, scald, the medicine of decubital ulcer infection or product.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102631699A (en) * | 2011-02-11 | 2012-08-15 | 佛山市优特医疗科技有限公司 | Antibacterial fiber dressing containing nanometer metal and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102631699A (en) * | 2011-02-11 | 2012-08-15 | 佛山市优特医疗科技有限公司 | Antibacterial fiber dressing containing nanometer metal and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| YUYUN ZHAO ET AL.: "Synergy of Non-antibiotic Drugs and Pyrimidinethiol on Gold Nanoparticles against Superbugs", 《JOURNAL OF THE AMERICAN CHEMICAL SOCIETY》 * |
| YUYUN ZHAO ET AL.: "Tuning the Composition of AuPt Bimetallic Nanoparticles for Antibacterial Application", 《ANGEW. CHEM. INT. ED.》 * |
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