CN1061352C - Extraction method of oil-tea camellia seed saponin - Google Patents
Extraction method of oil-tea camellia seed saponin Download PDFInfo
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- CN1061352C CN1061352C CN97100513A CN97100513A CN1061352C CN 1061352 C CN1061352 C CN 1061352C CN 97100513 A CN97100513 A CN 97100513A CN 97100513 A CN97100513 A CN 97100513A CN 1061352 C CN1061352 C CN 1061352C
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- camellia seed
- oil
- saponin
- seed saponin
- tea
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Abstract
The present invention relates to a method for extracting oiltea camellia seed saponin. Oiltea camellia seed cake dregs are used as raw material, ethanol and water are used as mixed solvent to extract the oiltea camellia seed cake dregs in a volume ratio of ethanol to water of 100: 5 to 100: 100, and filtrate is added to aceton solvent to be precipitated to prepare oiltea camellia seed saponin. The method has the advantages of simple technology and low cost, and the prepared oiltea camellia seed saponin has purity as high as 85% to 95%.
Description
The present invention relates to a kind of tea saponin extracting method, particularly a kind of method of from the tea seed grouts, extracting saponin.
Contain multiple organism in the tea seed grouts, wherein extract highly purified oil-tea camellia seed saponin and be the key issue in the comprehensive utilization, Su 957910 discloses the employing ethylene dichloride and has made solvent, 60-70 ℃ of method that makes oil-tea camellia seed saponin, this method has toxicity because of solvent for use, is difficult for promoting the use of.CN 1068360A discloses with after the pure aqueous solution extracting, makes oil-tea camellia seed saponin through decolouring, deodorization, evaporate to dryness technology, and this technology has complex process and the low shortcoming of gained saponin purity.
The present invention has overcome that extraction solvent has toxicity, complex process and the low shortcoming of products therefrom purity in the prior art, and a kind of method that adopts coprecipitation mode to prepare the high purity oil-tea camellia seed saponin is provided.
The present invention is to be raw material with the cake of camellia oleifera seeds that newly squeezes behind the tea oil, adding the doubly pure water mixed solvent of material quantity 1.2-5 in raw material extracts, alcohol is 100: 5～100: 100 with water volume ratio in its pure water mixed solvent, at 75-85 ℃ of backflow 1-5 hour, preferable reflux temperature was 75-80 ℃, is preferably 2-4 hour, after filtration, after removing slag, filtrate poured in its volume 2-5 ketone solvent doubly precipitate, its throw out drying makes oil-tea camellia seed saponin.Its purity is 85-95%, and its productive rate is the 6-12% of raw material input amount.
Oil-tea camellia seed saponin extraction process of the present invention has simple to operate, the advantage that cost is low, the saponin hydrolysis reaction of having avoided the high temperature evaporate to dryness to produce, particularly adopt the precipitator method, make product purity reach 90-95%, the oil-tea camellia seed saponin that extracts detects through IR, IU, is the triterpenoid saponin mixture of sugared body A and theasapogenol B composition, and its structural formula is as follows:
A B
Embodiment 1:
In the 1kg cake of camellia oleifera seeds, add 5l Fatty Alcohol(C12-C14 and C12-C18) and 0.5l water, reflux at 75-85 ℃ and filtered in 1 hour, remove slag, it is 0.3-3l that filtrate under reduced pressure is concentrated into volume, pours in 11 acetone cooling postprecipitation thing into, through centrifugal, the dry oil-tea camellia seed saponin 90g that gets, wherein oil-tea camellia seed saponin purity is 95%.
Embodiment 2:
In the 1.5kg cake of camellia oleifera seeds, add 3l ethanol and 0.3l water, reflux and stayed filtrate in 2 hours, adding 2l ethanol and 0.2l water again refluxed 1.5 hours, filter and remove residue, merging filtrate, be concentrated into 0.625l solution, pour in the 2.6l acetone and precipitate, after filtration, centrifugal, dry purity is 93% oil-tea camellia seed saponin 170g.
Embodiment 3:
In the 1kg cake of camellia oleifera seeds, add 5l Fatty Alcohol(C12-C14 and C12-C18) and 0.5l water, reflux at 75-85 ℃ and filtered in 1 hour, remove slag, it is 0.3-3l that filtrate under reduced pressure is concentrated into volume, pours in the 3.11l acetone cooling postprecipitation thing into, through centrifugal, the dry oil-tea camellia seed saponin 144g that gets, wherein oil-tea camellia seed saponin purity is 95%.
Claims (1)
1, a kind of extracting method of oil-tea camellia seed saponin comprises extracting, filtration process, it is characterized in that described extracting method, and step is carried out in the following order:
(1) in the cake of camellia oleifera seeds after raw material newly squeezes tea oil, adding volume ratio is that 100: 5～100: 100 pure water mixed solvents carry out extracting, and raw material and mixed solvent amount ratio are 1: 1.2～5.0, at 75-85 ℃ of stirring and refluxing 1-5 hour,
(2) above-mentioned filtrate is poured in the acetone solvent precipitate, its throw out drying makes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100513A CN1061352C (en) | 1997-01-24 | 1997-01-24 | Extraction method of oil-tea camellia seed saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100513A CN1061352C (en) | 1997-01-24 | 1997-01-24 | Extraction method of oil-tea camellia seed saponin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1188772A CN1188772A (en) | 1998-07-29 |
| CN1061352C true CN1061352C (en) | 2001-01-31 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97100513A Expired - Fee Related CN1061352C (en) | 1997-01-24 | 1997-01-24 | Extraction method of oil-tea camellia seed saponin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1061352C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1098349C (en) * | 2000-12-21 | 2003-01-08 | 黄宝生 | Process for purifying tea seed oil with plant juice |
| CN108997471B (en) * | 2018-09-17 | 2021-02-19 | 中国科学院青岛生物能源与过程研究所 | Green preparation method of high-purity tea saponin |
| CN109293728A (en) * | 2018-10-09 | 2019-02-01 | 中国科学院青岛生物能源与过程研究所 | A kind of green low cost preparation method of high-purity tea saponin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106634A (en) * | 1986-09-19 | 1987-09-02 | 核工业部第六研究所 | A kind of cake of camellia oleifera seeds comprehensive utilization process |
| CN1040592A (en) * | 1989-05-06 | 1990-03-21 | 核工业第六研究所 | From pressed cake of camellia seeds, extract the process for purification of saponin |
| CN1068360A (en) * | 1991-07-03 | 1993-01-27 | 湖南师范大学 | From oil tea (tea-seed) grouts, extract the technology of tea oil and saponin |
| CN1080291A (en) * | 1992-06-26 | 1994-01-05 | 郴州科技实业公司 | From tea waste, extract the method for refined saponin |
-
1997
- 1997-01-24 CN CN97100513A patent/CN1061352C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106634A (en) * | 1986-09-19 | 1987-09-02 | 核工业部第六研究所 | A kind of cake of camellia oleifera seeds comprehensive utilization process |
| CN1040592A (en) * | 1989-05-06 | 1990-03-21 | 核工业第六研究所 | From pressed cake of camellia seeds, extract the process for purification of saponin |
| CN1068360A (en) * | 1991-07-03 | 1993-01-27 | 湖南师范大学 | From oil tea (tea-seed) grouts, extract the technology of tea oil and saponin |
| CN1080291A (en) * | 1992-06-26 | 1994-01-05 | 郴州科技实业公司 | From tea waste, extract the method for refined saponin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1188772A (en) | 1998-07-29 |
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