CN1061241C - Medicine for treating diabetes - Google Patents

Abstract

The present invention relates to a medicine for treating diabetes, more specifically a tea bag prepared by using Chinese medicinal herb as an effective component. The medicine is prepared from common anemarrhena rhizome, ladybell root, raw rehmannia root, raw yam, cornus, alismatis, epimedium sagittatum and red sage root, has the functions of nourishing yin, tonifying the kidney, reducing fever, activating blood circulation, and benefiting qi for promoting production of fluid, and is suitable for treating diabetes with main symptoms of extreme heat because of yin asthenia, and deficiency of both qi and yin.

Description

A kind of medicine for the treatment of diabetes

The present invention relates to a kind of medicine for the treatment of diabetes, specifically be a kind of be the Chinese medicine that effective ingredient is prepared from the Chinese herbal medicine, this medicine has good blood fat reducing, hypoglycemic effect, belongs to the field of Chinese medicines.

Diabetes are a kind of common frequently-occurring diseases.At the end of the seventies, 300,000 census of the populations of 14 provinces and cities of China find that diabetes (diabetes) prevalence only is 13.4 ‰.And find that according to Beijing, Liaoning and the investigation of Shanxi three provinces and cities diabetes prevalence has reached 24.6 ‰ the year ends 80; It was also reported, 60～70 years old age group diabetes prevalence of China by 1981 64 ‰, risen to 125 ‰ by 1991, increased by one times during the decade.Only Beijing area nineteen ninety sampling survey shows, among the crowd, diabetes prevalence has the trend of rising again in recent years up to 53.0 ‰ more than 40 years old.According to the authoritative sources, conservative estimation, only the diabetics of China can reach more than 1,000 ten thousand people at present.According to the international diabetes association report that is located at Brussels, the investigation of The World Health Organization (WHO) shows that the diabetics number worldwide increases sharply.The onset diabetes rate of Eastern Mediterranean, the Middle East, Southeast Asia and West Pacific region increases more greatly.In these areas, adult population's 20% is subjected to the invasion and attack of this disease, wherein in some place, and this ratio even up to 50%.Report that the whole world at present nearly 10,000 ten thousand (100,000,000) people 6% of the population of promptly growing up suffers from diabetes.Diabetes are the third-largest common dead factors in industrialized country, are only second to heart disease and cancer.Therefore, invent a kind of taking convenience and eutherapeutic medicine is extremely urgent.

The object of the invention provides a kind of taking convenience and the effective Chinese medicine of treatment diabetes.

Medicine of the present invention is that the inventor carries out dialectical opinion according to theory of Chinese medical science and controls, and the legislation prescription develops in conjunction with modern preparation process.

Another object of the present invention provides a kind of medicinal tea of treatment diabetes of taking convenience.

Chinese medicine and pharmacy has experienced very long process to the understanding of diabetes (diabetes), and in the successive dynasties development is arranged gradually.The present age Chinese medicine and pharmacy then more system is comprehensive from whole opinion to the understanding of diabetes (diabetes).Put it briefly, the cause of disease have throw off restraint eat delicious food, the five kinds of factors such as drink, five mentally disturbed too urgent, intemperate sexual intercourse or calentura fire are dry of accumulating in the course of time; Pathogenesis has two burnt internal organs opinions, extreme misery theory of the Yin and Yang, the specimen deficiency and excess is distinguished and change of disease theory.Wherein emphasis is high in the part of the body cavity above the diaphragm housing the heart and lungs death by fire dryness of the lung, the middle burnt deficiency-heat, the first empty consumption of kidney of lower warmer.The kidney being a ZANG-organ containing both water and fire, the extreme misery imbalance of YIN and YANG has substantial connection in the card of particularly human body root of YIN and YANG, so three types of diabetes and the kidney.In a word, quenching one's thirst then is this with the deficiency of YIN, scorching for marking; Among weakened body resistance, be basis to suffer from a deficiency of the kidney again; And among suffering from a deficiency of the kidney, the deficiency of YIN is normal, and fire declines for becoming.So treatment diabetes (diabetes) is though answer differentiation of symptoms and signs for classification of syndrome, fundamentum should be enriching yin and nourishing kidney and supplementing QI for promoting the production of body fluid is laid equal stress on, and is equipped with heat clearing away again, invigorates blood circulation and wait until flavour of a drug, can receive satisfied effect surely.

The legislation principles of medicine of the present invention is enriching yin and nourishing kidney, heat clearing and blood circulation promoting, supplementing QI for promoting the production of body fluid.

Drug component of the present invention and weight proportion are as follows:

Rhizoma Anemarrhenae 15-5 weight portion

Radix Adenophorae 15-5 weight portion

Radix Rehmanniae 10-2 weight portion

Rhizoma Dioscoreae 6-2 weight portion

Fructus Corni 6-2 weight portion

Rhizoma Alismatis 5-2 weight portion

Herba Epimedii 20-5 weight portion

Radix Salviae Miltiorrhizae 20-5 weight portion.

The preferred weight proportioning of each component is in the medicine of the present invention:

Rhizoma Anemarrhenae 12-8 weight portion

Radix Adenophorae 12-8 weight portion

Radix Rehmanniae 8-4 weight portion

Rhizoma Dioscoreae 5-3 weight portion

Fructus Corni 5-3 weight portion

Rhizoma Alismatis 4-2 weight portion

Herba Epimedii 15-10 weight portion

Radix Salviae Miltiorrhizae 15-10 weight portion.

The optimum weight proportioning of drug component of the present invention is:

The Rhizoma Anemarrhenae 12 weight portions

Radix Adenophorae 12 weight portions

Radix Rehmanniae 8 weight portions

Rhizoma Dioscoreae 4 weight portions

Fructus Corni 4 weight portions

Rhizoma Alismatis 3 weight portions

Herba Epimedii 10 weight portions

Radix Salviae Miltiorrhizae 12 weight portions.

Preparation technique according to routine, medicine of the present invention can be prepared into any pharmaceutical preparation that is suitable for clinical use, for example, medicinal tea, pill, tablet, powder, injection, oral liquid, capsule, suspensoid, unguentum, granule, injectable powder, masticatory etc.

According to the needs of preparation, can also contain drug excipient commonly used in the pharmaceutics field in the medicine of the present invention, for example, solvent, filler, flavoring agent, disintegrating agent, binding agent, lubricant, wetting agent, pigment, solubilizing agent, diluent, thickening agent etc.

The preferred dosage form of medicine of the present invention is a medicinal tea, and medicinal tea is excipient usually with the tea dust.Folium Camelliae sinensis is for a long time all as a kind of health beverage.Modern scientific research proves: the contained theaflavin of Folium Camelliae sinensis can combine with infectious nucleic acid, can hinder the activity of virus.Folium Camelliae sinensis can heat clearing away, make eye bright, diuresis.But as the excipient of this medicine, its diuresis can make renal tubules, glomerular filtration accelerate, and number of micturitions and voided volume increase, and equal " to make the matter worse " to the kidney disorder patient.Therefore, choice of drug Folium Camelliae sinensis of the present invention is unaccommodated as excipient.

Herba Epimedii is the Herba indigoferae Pseudotinctoriae in the prescription.With this broken leaf coarse powder is excipient, except that can overcoming Folium Camelliae sinensis diuretic disadvantage, can also play the effect of " kidney-Yang activating QI ".This medicine contains flavones ingredients such as icariine and noricariin, has the effect of androgen sample, meets Chinese medicine invigorating kidney, promoting blood circulation theory.Other has blood sugar lowering and effect for reducing blood fat.

Herba Epimedii coarse powder consumption size directly influences the pack amount of teabag.Want little principle according to medication dose under the prerequisite that guarantees drug effect, the general custom of drinking tea with reference to people, in conjunction with every agent medicine go out (doing) cream rate, the inventory of selected Herba Epimedii coarse powder be 30% of this drug dose, reaches the requirement of writing out a prescription and stipulating.

Medicine medicinal tea preparation technology of the present invention is as follows:

1, takes by weighing recipe quantity Rhizoma Anemarrhenae decoction pieces and put in the extraction pot, add 7 times of 70% soak with ethanol half an hour of amount, refluxed the leaching medicinal liquid 1 hour.Medicinal residues add 6 times of amount 70% alcohol refluxs 1 hour again, filter.Medicinal residues discard, and merge medicinal liquid twice, place 24 hours for 0～5 ℃.Leaching cold preservation medicinal liquid is put in the alembic, reclaims ethanol to the greatest extent, continues to be concentrated into thick paste (d=1.4) and emits.Vacuum drying below 80 ℃.Get yellow crisp dry thing, weigh airtight preservation;

2, Herba Epimedii is selected totally, got its " leaf " and pulverize out this dose 30% coarse powder, cross 20 mesh sieves, standby behind the dry sterilization; All the other (70%) are gone into liquid medicine such as Radix Adenophorae and are boiled.

3, press recipe quantity Radix Adenophorae and Radix Rehmanniae two are distinguished the flavor of the medicines elutriation totally, drop in the extraction pot, add the Herba Epimedii of surplus, the deionized water immersion half an hour with 10 times of amounts, behind the Rhizoma Dioscoreae of adding rinsing, decocted the leaching medicinal liquid 1.5 hours.The deionized water that medicinal residues add 8 times of amounts again decocted 1 hour, filtered, and discarded medicinal residues.The extracting solution that merges twice filtering changes in the concentration tank, and the volume that is concentrated into suitable spice amount (1 times) is emitted and cooled.Under agitation slowly add dense alcohol, make to contain the alcohol amount and reach 70%, cold preservation was left standstill 72 hours.The clear and bright medicinal liquid of leaching changes in the alembic, and decompression recycling ethanol is not emitted after having the alcohol flavor.Clean alembic with small amount of deionized water.Merging filtrate and washing liquid, standby.

4, the Fructus Corni of recipe quantity, Rhizoma Alismatis, Radix Salviae Miltiorrhizae rinsing are packed into after clean extraction pot, refluxed one hour after half an hour with 60% soak with ethanol of 8 times of medical material amounts.Leaching medicinal liquid, medicinal residues add the pure liquid of 6 amounts again and refluxed one hour.Filter, discard medicinal residues, merge backflow twice, cold preservation was placed 48 hours.The clear and bright medicinal liquid of leaching changes in the extraction pot, and decompression recycling ethanol is to the greatest extent, emit medicinal liquid after, clean alembic with small amount of deionized water, merge washing liquid and medicinal liquid, standby.

5, merge (3) and (4) two medicinal liquids, vacuum concentration is admixed the Herba Epimedii coarse powder to being thick paste, emitting, behind the mixing in vacuum drying below 80 ℃.Take out dry thing (water content 3～5%) and, be ground into coarse powder, granulate behind 20 mesh sieves excessively with the Rhizoma Anemarrhenae extract uniform mixing.Inspect by ready samples qualified after, be packed as every bag 4.5 the gram bag tea agent.

Medicine of the present invention has enriching yin and nourishing kidney, heat clearing and blood circulation promoting, supplementing QI for promoting the production of body fluid function, is applicable to that intense heat due to deficiency of YIN card, deficiency of both QI and YIN disease are main diabetes (diabetes).

The advantage of medicine medicinal tea of the present invention is a taking convenience, in conjunction with the characteristics of diabetic polydipsia water, makes teabag, borrows patient's drinking-water to take medicine, and has both reached the purpose of treatment, does not have added burden again.

Following zoopery has confirmed the drug action of medicine of the present invention (the clean teabag of the commodity sugar that disappears by name).

The disappear blood sugar reducing function experiment of the clean teabag of sugar of experimental example 1:

1, animal: 17～23g is male for NIH kind mice.

2, medicine:

1. the clean teabag of the sugar that disappears is by China Medical Science Foundation's tcm development center development.

2. (Glibenclamide, HB-419), specification is the 2.5mg/ sheet to glibenclamide tablet, adult's a slice every day.(positive control drug).

3. streptozotocin (Streptozotocin): FlukaAG CH-9470 Buchs.

4. Glucose oxidase reagent: SIGA Fluka, the Shanghai Biochemical Research provides.

3, experimental technique:

1. this experiment adopts chain urea toxin (STZ) to make the mice diabetes model.Promptly get 50 of mices, be divided into 5 groups, each numbering of only weighing.Matched group intraperitoneal injection of saline 0.15ml/10g, all the other 4 groups equal lumbar injection STZ150mg/kg (1% streptozotocin is 0.15ml/10g) after 48 hours, pluck a branch hole ball and get blood, measure blood sugar content, are blood sugar content before the administration.

2. after the model success, matched group and model group only gavage normal saline 0.4ml/ every day; Sugar clean (heavy dose) group that disappears is only irritated stomach teabag (" tea ") 0.4ml/ every day; Sugar clean (low dose) group that disappears is only irritated stomach teabag (" tea ") 0.2ml/ every day; The glyburide group gavages glyburide 5mg/kg every day.Each organized successive administration 10 days, and fasting was won other branch hole ball and got blood after 12 hours, measured blood sugar content.Carry out mathematical statistics and handle, relatively between each group zero difference is arranged.

3. teabag (" tea ") preparation: take by weighing teabag 6g (quite 21.3g spice) and put into beaker, add hot boiled water 20ml, make its dissolving fully, cool standby.This " tea " 1ml ≌ 1.07g spice, 0.4ml ≌ 0.43g spice.By mice average weight 20g/ only, body weight 60kg calculates for each person, then differs 3000 times, is 20 times of people's dosages (heavy dose) and 10 times (low dose) through conversion experimental drug amount.

4, experimental result:

The clean tea bag of the sugar that disappears sees Table I and table II to the hypoglycemic activity of streptozotocin (STZ) modeling type mice.

Table I: the blood sugar content of each group before the administration

Group	Number of animals	Body weight (g)	Blood sugar content mg/100ml
				Two two tea (20) times glyburide 5mg/kg that fall of tea (20 times) that fall of matched group model group	10 7 10 10 10	20.7±2.1 19.7±1.8 19.9±2.3 19.8±1.3 19.5±2.0	157.9±47.8 260.8±40.2▲▲▲ 225.7±66.0▲ 241.5±49.9▲▲ 249.2±70.7▲▲

Table II: the blood sugar content of each group after the administration

Group	Number of animals	Body weight (g)	Blood sugar content mg/100ml
				Two two tea (10) times glyburide 5mg/kg that fall of tea (20 times) that fall of matched group model group	9 7 6 6 6	22.1±2.1 18.0±1.4 19.2±1.6 17.5±2.0 19.3±2.1	111.5±46.5 262.6±130.1★★★ 117.1±21.1★ 172.3±77.1★ 212.9±66.9★

Annotate: ▲ ▲ ▲ and ★ ★ ★ and matched group relatively P＜0.001 ▲ ▲ with matched group P＜0.01 ▲ compare P＜0.05 ★ and model group compares P＜0.015, discussion relatively with matched group

(1), show from above-listed data: the clean teabag of the sugar that disappears is to having hypoglycemic activity preferably by STZ moulding mice, and action intensity is similar to glyburide (5mg/kg); And between two groups of clean teabag of the sugar that disappears tangible dose-effect relationship is arranged.

(2), to the influence of mice body weight: this laboratory observation can cause mice polyuria, weight loss to streptozotocin; Clean teabag (heavy dose) group of the sugar that disappears and glyburide group mice urinate seldom, therefore keep former body weight substantially, but remarkable inadequately with the model group comparing difference, may be short relevant with the administration natural law.

(3), after the administration to the observation of mice amount of drinking water; This laboratory observation is 30～80ml/10 to normal (matched group) mice amount of drinking water every day; Model group amount of drinking water every day is 50～120ml/10; Each administration group amount of drinking water is 30～100ml/10, and its amount of drinking water is close with normal (matched group) mice, and illustrating has certain improvement effect to symptom (polydipsia).

The disappear lipid-lowering test of the clean teabag of sugar of experimental example 2:

The diabetics majority can cause blood fat rising in various degree often with disorders of lipid metabolism, so most of clinically glycosuria patient (non-late period) Chang Bingfa " obesity ".This experiment causes hyperlipidemia rat animal model with hypercholesterolemia and high triglyceride feedstuff, and is positive to making medicine with gemfibrozil (G emfi-brozil), observes the effect for reducing blood fat of the clean teabag of sugar that disappears.

1, laboratory animal: Wistar kind, male white rat, body weight 150～200g.

2, high lipid food: 2%Chal, 0.5% cholic acid, 0.2% methylthiouracil, 10% Adeps Sus domestica, 5% sucrose and 82.3% normal feedstuff.

3, medicine:

1., the clean teabag of sugar disappears: make " tea " by last experiment, irritate stomach with body weight 25mg/kg dosage, 20 times of the dosage of quite being grown up;

2., gemfibrozil: U.S. China sodium company Pai De pharmaceutical factory product, each capsule contains G em-fiberzil300mg, gives rat oral gavage with 13.9mg/kg (body weight) dosage.

4, reagent:

1., cholesterol elisa reagent (three enzyme cocktails): Shanghai Institute of Pharmaceutical Industry and Shanghai the 18 pharmaceutical factory's product;

2., sodium cholate: Sigma product, import packing;

3., cholesterol: EMerck import packing.

5, experimental technique:

Get 36 of Wistar male rats, after feeding observation normally, be divided into 4 groups at random, 9 every group.I group: normal control group; II group: model group; III group: the two tea groups of falling of glycolipid; IV group: gemfibrozil (contrast medicine) group.Then II, III, three groups of IV rat are added and feed " high lipid food ", every rat 20g every day; In addition, III, two groups of rats of IV are fed simultaneously the medicine of this group regulation.Feed after 15 days, get the every data of hematometry.

6, mensuration project and method:

1., serum total cholesterol assay: press the Allain method, revise slightly through the experimenter, its enzyme reagent is pressed the preparation of reagent description;

2., serum triglycerides assay; The acetylacetone,2,4-pentanedione color reaction;

3., serum high-density LP-cholesterol HDL-Ch measures: heparin-Mn ⁺⁺The sedimentation method.

7, result:

By above-mentioned experimental technique, measure after 15 days.Hunger is 12 hours before measuring, etherization, heart extracting blood.Measurement result sees Table III:

Table III clean (tea) blood fat reducing result of sugar that disappears

Group	The example number	T-CHOL mg/dl (X ± SD)	Triglyceride mg/dl (X ± SD)	HDL-10h mg/dl (X±SD)	LDL-Ch mg/dl (X±SD)
						I group II group III group IV group	6 10 9 11	148.0±14.8 408.8±76.5 113.4±43.5 285.0±35.1	77.1±14.6 185.0±81.7 48.5±15.3 96.9±27.3	20.8±5.4 24.4±5.7 79.3±13.1 37.4±4.8	122.5±15.7 322.0±47.6 29.9±17.6 265.6±30.9

8, conclusion:

1., as can be seen: model group (II) and normal control group (I) be T-CHOL, triglyceride and HDL-Ch value P＜1.001 relatively, and it is quite successful that illustrative experiment is made hyperlipemia model from table III data that learn to handle by statistics;

2., two fall tea (III) and model group (II) relatively the P value of its T-CHOL, triglyceride, HDL-Ch, LDL-Ch illustrate that all less than 0.001 tea has highly significant to hyperlipidemia rats preventive and therapeutic effect pair falls in glycolipid;

3., gemfibrozil group (IV) and model group (II) relatively, its P value illustrates that less than 0.001 or 0.01 the credibility of this experiment is quite high;

4., two tea (III) group and model group (IV) are fallen relatively, the P of rising HDL-Ch＜0.001 illustrates that highly significant is better than matched group (gemfibrozil group).

In sum, this experimental result shows: the effect highly significant of the clean teabag of sugar to the serum total cholesterol that reduces the hyperlipidemia rat, triglyceride, LDL-Ch disappears; The level of its rising rat HDL-Ch has surpassed blood lipid-lowering medicine gemfibrozil (P＜0.001) produced in USA.Proof disappears the clean teabag of sugar according to goodish blood fat reducing and hypoglycemic activity are arranged, and can be clinical prevention diabetes and complication provides a kind of drug effect significant new product of Chinese medicine.

Embodiment

Rhizoma Anemarrhenae 4000g Radix Adenophorae 4000g

Radix Rehmanniae 2667g Rhizoma Dioscoreae 1333g

Fructus Corni 1333g Rhizoma Alismatis 1000g

Herba Epimedii 3333g Radix Salviae Miltiorrhizae 4000g

Method for making:

1, takes by weighing recipe quantity Rhizoma Anemarrhenae decoction pieces and put in the extraction pot, add 7 times of 70% soak with ethanol half an hour of amount, refluxed the leaching medicinal liquid 1 hour.Medicinal residues add 6 times of amount 70% alcohol refluxs 1 hour again, filter.Medicinal residues discard, and merge medicinal liquid twice, place 24 hours for 0～5 ℃.Leaching cold preservation medicinal liquid is put in the alembic, reclaims ethanol to the greatest extent, continues to be concentrated into thick paste (d=1.4) and emits.Vacuum drying below 80 ℃.Get yellow crisp dry thing, weigh airtight preservation;

2, Herba Epimedii is selected totally, got its " leaf " and pulverize out this dose 30% coarse powder, cross 20 mesh sieves, standby behind the dry sterilization; All the other (70%) are gone into liquid medicine such as Radix Adenophorae and are boiled.

3, press recipe quantity Radix Adenophorae and Radix Rehmanniae two are distinguished the flavor of the medicines elutriation totally, drop in the extraction pot, add the Herba Epimedii of surplus, with the deionized water immersion half an hour of 10 times of amounts.After adding the Rhizoma Dioscoreae of rinsing, decocted the leaching medicinal liquid 1.5 hours.The deionized water that medicinal residues add 8 times of amounts again decocted 1 hour, filtered, and discarded medicinal residues.The extracting solution that merges twice filtering changes in the concentration tank, and the volume that is concentrated into suitable spice amount (1 times) is emitted and cooled.Under agitation slowly add dense alcohol, make to contain the alcohol amount and reach 70%, cold preservation was left standstill 72 hours.The clear and bright medicinal liquid of leaching changes in the alembic, and decompression recycling ethanol is not emitted after having the alcohol flavor.Clean alembic with small amount of deionized water.Merging filtrate and washing liquid, standby.

4, the Fructus Corni of recipe quantity, Rhizoma Alismatis, Radix Salviae Miltiorrhizae rinsing are packed into after clean extraction pot, refluxed one hour after half an hour with 60% soak with ethanol of 8 times of medical material amounts.Leaching medicinal liquid, medicinal residues add the pure liquid of 6 amounts again and refluxed one hour.Filter, discard medicinal residues, merge backflow twice, cold preservation was placed 48 hours.The clear and bright medicinal liquid of leaching changes in the extraction pot, and decompression recycling ethanol is to the greatest extent, emit medicinal liquid after, clean alembic with small amount of deionized water, merge washing liquid and medicinal liquid, standby.

5, merge (3) and (4) two medicinal liquids, vacuum concentration is admixed the Herba Epimedii coarse powder to being thick paste, emitting, behind the mixing in vacuum drying below 80 ℃.Take out dry thing (water content 3～5%) and, be ground into coarse powder, granulate behind 20 mesh sieves excessively with the Rhizoma Anemarrhenae extract uniform mixing.Inspect by ready samples qualified after, be packed as every bag 4.5 the gram bag tea agent.

Claims (4)

1. pharmaceutical composition for the treatment of diabetes is characterized in that the component of said composition and weight proportion are:

Rhizoma Anemarrhenae 15-5 weight portion

Radix Adenophorae 15-5 weight portion

Radix Rehmanniae 10-2 weight portion

Rhizoma Dioscoreae 6-2 weight portion

Fructus Corni 6-2 weight portion

Rhizoma Alismatis 5-2 weight portion

Herba Epimedii 20-5 weight portion

Radix Salviae Miltiorrhizae 20-5 weight portion.

2. pharmaceutical composition according to claim 1 is characterized in that the component of said composition and weight proportion are:

Rhizoma Anemarrhenae 12-8 weight portion

Radix Adenophorae 12-8 weight portion

Radix Rehmanniae 8-4 weight portion

Rhizoma Dioscoreae 5-3 weight portion

Fructus Corni 5-3 weight portion

Rhizoma Alismatis 4-2 weight portion

Herba Epimedii 15-10 weight portion

Radix Salviae Miltiorrhizae 15-10 weight portion.

3. pharmaceutical composition according to claim 2 is characterized in that the component of said composition and weight proportion are:

The Rhizoma Anemarrhenae 12 weight portions

Radix Adenophorae 12 weight portions

Radix Rehmanniae 8 weight portions

Rhizoma Dioscoreae 4 weight portions

Fructus Corni 4 weight portions

Rhizoma Alismatis 3 weight portions

Herba Epimedii 10 weight portions

Radix Salviae Miltiorrhizae 12 weight portions.

4. medicine for the treatment of diabetes is characterized in that this medicine is the medicinal tea that is prepared from following compositions and method for making:

Rhizoma Anemarrhenae 4000g

Radix Adenophorae 4000g

Radix Rehmanniae 2667g

Rhizoma Dioscoreae 1333g

Fructus Corni 1333g

Rhizoma Alismatis 1000g

Herba Epimedii 3333g

Radix Salviae Miltiorrhizae 4000g

Method for making:

(1), take by weighing recipe quantity Rhizoma Anemarrhenae decoction pieces and put in the extraction pot, add 70% soak with ethanol half an hour of 7 times of amounts, refluxed 1 hour, leaching medicinal liquid, medicinal residues add 6 times of amount 70% alcohol refluxs 1 hour again, filter, medicinal residues discard, and merge medicinal liquid twice, place 24 hours for 0～5 ℃, leaching cold preservation medicinal liquid is put in the alembic, reclaims ethanol to the greatest extent, continues to be concentrated into density and is the thick paste of d=1.4 and emit, vacuum drying below 80 ℃, get yellow crisp dry thing, weigh airtight preservation;

(2), Herba Epimedii is selected totally, get its leaf and pulverize out this dose 30% coarse powder, cross 20 mesh sieves, standby behind the dry sterilization; All the other 70% are gone into liquid medicine such as Radix Adenophorae and boil;

(3), pressing recipe quantity eluriates Radix Adenophorae and Radix Rehmanniae two flavor medicines clean, drop in the extraction pot, the Herba Epimedii that adds surplus, deionized water with 10 times of amounts soaks half an hour, behind the Rhizoma Dioscoreae of adding rinsing, decocts 1.5 hours, the leaching medicinal liquid, the deionized water that medicinal residues add 8 times of amounts again decocted 1 hour, filtered, and discarded medicinal residues, the extracting solution that merges twice filtering, change in the concentration tank, the volume that is concentrated into 1 times of suitable spice amount is emitted and is cooled, and under agitation slowly adds dense alcohol, make and contain alcohol amount and reach 70%, cold preservation was left standstill 72 hours, and the clear and bright medicinal liquid of leaching changes in the alembic, decompression recycling ethanol is not emitted after having the alcohol flavor, clean alembic with small amount of deionized water, merging filtrate and washing liquid, standby;

(4), the Fructus Corni with recipe quantity, Rhizoma Alismatis, the Radix Salviae Miltiorrhizae rinsing extraction pot of packing into after clean,, refluxed one hour after half an hour with 60% soak with ethanol of 8 times of medical material amounts, leaching medicinal liquid, medicinal residues add the pure liquid of 6 amounts again and refluxed one hour, filter, discard medicinal residues, merge backflow twice, cold preservation was placed 48 hours, the clear and bright medicinal liquid of leaching changes in the extraction pot, decompression recycling ethanol is to the greatest extent, emit medicinal liquid after, clean alembic with small amount of deionized water, merge washing liquid and medicinal liquid, standby;

(5), merge 3 and 4 liang of medicinal liquids, vacuum concentration is admixed the Herba Epimedii coarse powder to being thick paste, emitting, behind the mixing in vacuum drying below 80 ℃ to water content 3～5%, take out dry thing, with the Rhizoma Anemarrhenae extract uniform mixing, be ground into coarse powder, granulate behind 20 mesh sieves excessively, packing promptly.