CN106119118A - A kind of method of two-part culture and improvement microalgae grease yield - Google Patents

A kind of method of two-part culture and improvement microalgae grease yield Download PDF

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Publication number
CN106119118A
CN106119118A CN201610543152.5A CN201610543152A CN106119118A CN 106119118 A CN106119118 A CN 106119118A CN 201610543152 A CN201610543152 A CN 201610543152A CN 106119118 A CN106119118 A CN 106119118A
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microalgae
reactor
culture
improvement
water
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高锋
李晨
冯丽娟
彭苑媛
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
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  • Medicinal Chemistry (AREA)
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  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A kind of method that the invention discloses two-part culture and improvement microalgae grease yield, in reactor, add culture fluid inoculate microalgae to working water level, controlling total nitrogen concentration is 340～380mg/L, total phosphorus concentration is 35～40mg/L, after inoculation, from water inlet pipe continuum micromeehanics input culture fluid to reactor, by suction filter pump, culture fluid in reactor is discharged reactor after hyperfiltration membrane assembly filters simultaneously, control inflow and water yield balance；After microdisk electrode to stable phase, water inlet will be changed tap water into, after continuum micromeehanics to total nitrogen concentration, total phosphorus concentration are down to 3～4.8mg/L, 0.4～0.8mg/L respectively, stop Inlet and outlet water operation, continue to cultivate 6～10 days, complete oil accumulation process.The present invention can be carried out in same reactor, operates relatively simple, it is simple to commercial production, can realize microalgae High Density Cultivation simultaneously and improve the target of microalgae grease content, improves the microalgae grease production capacity of system.

Description

A kind of method of two-part culture and improvement microalgae grease yield
Technical field
The present invention relates to microdisk electrode technical field, especially relate to a kind of two-part culture and improvement microalgae grease and produce The method of amount.
Background technology
The height of the fat content stored in microalgae body depends primarily on kind and the condition of culture of microalgae.Although People can be by microalgae body by the work such as Strain selection and training systern the most in laboratory conditions Fat content be accumulate to the high level of about 60%-70% of dry cell weight.But at extensive microdisk electrode Under engineering specifications, due to the restriction of condition of culture, the fat content of microalgae does not reaches far away the level in laboratory.
It addition, there is also a bigger obstacle at present: micro algae growth speed and the increasing of individual fat content Add and can not synchronize to carry out, thus cause the overall grease production speed of microalgae to effectively improve.
Summary of the invention
The present invention is to provide for a kind of method of two-part culture and improvement microalgae grease yield, can be in same reaction Carry out in device, operate relatively simple, it is simple to commercial production, microalgae High Density Cultivation can be realized simultaneously and improve micro- The target of algae fat content, can improve the microalgae grease production capacity of system.
To achieve these goals, the present invention is by the following technical solutions:
The method of a kind of two-part culture and improvement microalgae grease yield of the present invention, concretely comprises the following steps: at reactor Interior addition culture fluid inoculates microalgae to working water level, and controlling total nitrogen concentration is 340～380mg/L, and total phosphorus is dense Degree is 35～40mg/L,；After inoculation, from water inlet pipe continuum micromeehanics input culture fluid to reactor, pass through simultaneously Culture fluid in reactor is discharged reactor after hyperfiltration membrane assembly filters by suction filter pump, controls inflow and water yield Balance；Water inlet will change tap water after microdisk electrode to stable phase into, continuum micromeehanics to total nitrogen concentration, total phosphorus are dense After degree is down to 3～4.8mg/L, 0.4～0.8mg/L respectively, stop Inlet and outlet water operation, continue to cultivate 6～10 days, Complete oil accumulation process.The present invention nitrogen and phosphorus that first the growth raising of microalgae is high in for reactor is born Lotus, promotes the fast-growth of microalgae, and the micro algae growth in question response device to higher concentration also enters stationary growth phase After, change the operation of original Inlet and outlet water, water inlet is changed into the nitrogen phosphorus that tap water quickly reduces in reactor in culture fluid Concentration, makes microalgae lack at nitrogen and coerces lower accelerated accumulation oils and fats, thus is realizing High Density Cultivation same of microalgae Shi Tigao microalgae grease content, improves the microalgae grease production capacity of system.
As preferably, described culture fluid is Nitrogen-and Phosphorus-containing sewage or micro-algae culture medium.Nitrogen-and Phosphorus-containing sewage can be that city is dirty Waste water after water factory's process or breeding wastewater, it is commonly used in the art, without particular/special requirement.
As preferably, microalgae inoculum density is 0.1～0.3g/L.
As preferably, described microalgae is chlorella, chlamydomonas or scenedesmus.
As preferably, during cultivation, illumination uses outdoor light source or artificial light source, the intensity of illumination at reactor outer wall It is 80～140 μm ol m-2s-1, during cultivation, reactor bottom carries out aeration, aeration intensity by aeration tube 0.5～1.0m3/(m3Min), the gas being passed through is CO2Gas and the mixed gas of air, wherein CO2Content 4～8%, reactor hydraulic load maintains 0.8m3/(m3·d).By to the considered critical of condition of culture with Fat content cumulative speed in the growth rate of guarantee microalgae and microalgae body, had both realized the Concentrated Compact of microalgae Cultivating, in making again microalgae body, fat content is accumulate to higher level, thus ensures grease production ability.
Therefore, there is advantages that first the growth of microalgae improves high nitrogen in for reactor Phosphorus nutrition load, promotes the fast-growth of microalgae, and the micro algae growth in question response device to higher concentration is gone forward side by side in meditation Only after trophophase, change the operation of original Inlet and outlet water, water inlet is changed into tap water and quickly reduces culture fluid in reactor In concentration of nitrogen and phosphorus, make microalgae nitrogen lack coerce lower accelerated accumulation oils and fats, whole process is in same reactor Carry out, operate relatively simple, it is simple to commercial production, improve micro-while realizing the High Density Cultivation of microalgae Algae fat content, can improve the microalgae grease production capacity of system.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of reactor in the present invention.
Fig. 2 is microalgae concentration in embodiment of the present invention incubation, fat content, the change of system oils and fats cumulant Change figure.
In figure: reactor 1, hyperfiltration membrane assembly 2, suction filter pump 3, aeration tube 4.
Detailed description of the invention
The present invention will be further described with detailed description of the invention below in conjunction with the accompanying drawings.
Embodiment
Using the Wastewater from Pig Farm after the removal of impurity is gone in precipitation filtration as culture fluid, its water quality is as clear as crystal, total nitrogen Concentration is 340mg/L, and total phosphorus concentration is 35mg/L, adds culture fluid and be followed by working water level in reactor Planting chlorella, inoculum density is 0.1g/L, and illumination uses outdoor light source, the intensity of illumination at reactor outer wall It is 80 μm ol m-2s-1；
After inoculation, from water inlet pipe continuum micromeehanics input culture fluid to reactor 1 (as shown in Figure 1), reactor Hydraulic load maintains 0.8m3/(m3D), pass through suction filter pump 3 by culture fluid in reactor through ultrafilter membrane simultaneously Assembly 2 discharges reactor after filtering, and controls inflow and water yield balance, and during cultivation, reactor bottom passes through Aeration tube 4 carries out aeration, aeration intensity 0.5m3/(m3Min), the gas being passed through is CO2Gas and air Mixed gas, wherein CO2Content 4%；
Water inlet will change tap water after microdisk electrode to stable phase into, reactor hydraulic load maintains 0.8m3/ (m3D), after continuum micromeehanics to total nitrogen concentration, total phosphorus concentration are down to 3mg/L, 0.4mg/L respectively, stop into Water outlet operates, and continues to cultivate 6 days, completes oil accumulation process, and during cultivation, reactor bottom carries out aeration, Aeration intensity 0.5m3/(m3Min), the gas being passed through is CO2Gas and the mixed gas of air, wherein CO2 Content 4%, reactor hydraulic load maintains 0.8m3/(m3·d)。
Microalgae concentration (mg/L), fat content (%), system oils and fats cumulant (microalgae concentration in incubation × fat content) change as shown in Figure 2.
Figure it is seen that after using the two-part of the present invention to cultivate, lack compared to nitrogen and coerce front microalgae Fat content can be promoted to rapidly the microalgae in about 56%, and simultaneous reactions device from 21.4 before～23.6% Concentration stills remain in about 3.2g/L, and therefore the oil accumulation of system has measured bigger raising, from 0.71g/L when 12 days is promoted to rapidly 1.83g/L when the 20th day, and these operations are all in same reaction Carry out in device, operate relatively simple, it is simple to commercial production.
Embodiment described above is the one preferably scheme of the present invention, and the present invention not makees any form On restriction, on the premise of without departing from the technical scheme described in claim, also have other variant and change Type.

Claims (5)

1. the method for a two-part culture and improvement microalgae grease yield, it is characterised in that concretely comprise the following steps: instead Adding culture fluid in answering device and inoculate microalgae to working water level, controlling total nitrogen concentration is 340～380mg/L, always Phosphorus concentration is 35～40mg/L；After inoculation, from water inlet pipe continuum micromeehanics input culture fluid to reactor, simultaneously By suction filter pump, culture fluid in reactor is discharged reactor after hyperfiltration membrane assembly filters, control inflow and go out Water balance；After microdisk electrode to stable phase, water inlet will be changed into tap water, continuum micromeehanics to total nitrogen concentration, total After phosphorus concentration is down to 3～4.8mg/L, 0.4～0.8mg/L respectively, stop Inlet and outlet water operation, continue to cultivate 6～10 My god, complete oil accumulation process.
The method of a kind of two-part culture and improvement microalgae grease yield the most according to claim 1, its feature exists In, described culture fluid is Nitrogen-and Phosphorus-containing sewage or micro-algae culture medium.
The method of a kind of two-part culture and improvement microalgae grease yield the most according to claim 1, its feature exists In, microalgae inoculum density is 0.1～0.3g/L.
The method of a kind of two-part culture and improvement microalgae grease yield the most according to claim 3, its feature exists In, described microalgae is chlorella, chlamydomonas or scenedesmus.
The method of a kind of two-part culture and improvement microalgae grease yield the most according to claim 1, its feature exists In, during cultivation, illumination uses outdoor light source or artificial light source, and the intensity of illumination at reactor outer wall is 80～140 μmol m-2s-1, during cultivation, reactor bottom carries out aeration, aeration intensity 0.5～1.0 by aeration tube m3/(m3Min), the gas being passed through is CO2Gas and the mixed gas of air, wherein CO2Content 4～8%, Reactor hydraulic load maintains 0.8m3/(m3·d)。
CN201610543152.5A 2016-07-07 2016-07-07 A kind of method of two-part culture and improvement microalgae grease yield Pending CN106119118A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106609246A (en) * 2017-01-19 2017-05-03 浙江海洋大学 Microalgae particle culture method with effect of fast precipitation harvest
CN110526414A (en) * 2019-09-29 2019-12-03 浙江海洋大学 For purifying the film-bioreactor and its application method of phenol wastewater
CN112280684A (en) * 2020-11-04 2021-01-29 山东建筑大学 Microalgae culture method for determining nitrogen supply torsion night grease loss based on photoperiod
CN112919641A (en) * 2021-03-23 2021-06-08 南京科盛环保技术有限公司 Method for removing nitrogen and phosphorus by using microalgae and treatment device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103789195A (en) * 2014-01-16 2014-05-14 浙江海洋学院 Membrane microalgae photobioreactor for realizing in-situ solid-liquid separation and culture method thereof
CN105132351A (en) * 2015-10-12 2015-12-09 山东大学 Method for rapidly accumulating lipid by high-temperature stress on micro-algae

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103789195A (en) * 2014-01-16 2014-05-14 浙江海洋学院 Membrane microalgae photobioreactor for realizing in-situ solid-liquid separation and culture method thereof
CN105132351A (en) * 2015-10-12 2015-12-09 山东大学 Method for rapidly accumulating lipid by high-temperature stress on micro-algae

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106609246A (en) * 2017-01-19 2017-05-03 浙江海洋大学 Microalgae particle culture method with effect of fast precipitation harvest
CN110526414A (en) * 2019-09-29 2019-12-03 浙江海洋大学 For purifying the film-bioreactor and its application method of phenol wastewater
CN110526414B (en) * 2019-09-29 2022-01-11 浙江海洋大学 Use method of membrane-photobioreactor
CN112280684A (en) * 2020-11-04 2021-01-29 山东建筑大学 Microalgae culture method for determining nitrogen supply torsion night grease loss based on photoperiod
CN112919641A (en) * 2021-03-23 2021-06-08 南京科盛环保技术有限公司 Method for removing nitrogen and phosphorus by using microalgae and treatment device
CN112919641B (en) * 2021-03-23 2024-01-16 南京科盛环保技术有限公司 Method and treatment device for denitrification and dephosphorization by utilizing microalgae

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