CN106116793A - A kind of high-efficient culture liquid of liquid plantation Fructus Cucumidis sativi and preparation method thereof - Google Patents

Abstract

The invention discloses high-efficient culture liquid of a kind of liquid plantation Fructus Cucumidis sativi and preparation method thereof, be made up of the raw material of following weight (part): algae mixed solution, potassium ferrate, aluminum sulfate, Herba Artemisiae Annuae slag, sodium citrate, manganese sulfate, lactic acid, biotin, calcium ammonium nitrate, potassium nitrate, EM microbial inoculum, magnesium sulfate, carbamide, bean cake powder, corn cob, Kaolin and appropriate water；According to nutritional need during green cucumber, adding appropriate nutrient, be dissolved in and rupture in the cell culture fluid as substrate with algae, this is the solution of a kind of colloid, flow velocity stirs relatively, there is the function promoting Nutrient Absorption, be greatly saved human resources and land resource, it is achieved high yield, simultaneously because need not earth, so reducing the unnecessary loss caused due to pest and disease damage, the most do not use pesticide, safety and sanitation.

Description

A kind of high-efficient culture liquid of liquid plantation Fructus Cucumidis sativi and preparation method thereof

Technical field

The invention belongs to the Technology field of liquid culture fluid, be specifically related to the efficient training of a kind of liquid plantation Fructus Cucumidis sativi Nutrient solution and preparation method thereof.

Background technology

The advantage of soilless culture is: use the nutrient matrix of liquid, can mineral nitrogen salt is directly dissolved in liquid by it's too late Water in, nutrient is provided the most at a flow way, crop is under the support not having soil, directly by Root Absorption nutrient, greatly Big saving manpower and land resource, the most also save fertilizer and the environmental problem avoiding fertilizer loss to cause；But liquid is cultivated The Railway Project of mechanism needs to promote, and will obtain bigger income.

The form of the Root Absorption nutrient of crops, mainly by the root hair zone of the system of fibrous root tip of a root in root system, by meristematic zone It is absorbed as main region with elongation zone, and the mode absorbed is directly to absorb the mineral ion of contact, for the process intercepted and captured, then lead to The ion channel crossed on the cell membrane of uptake zone cell optionally passes through, and these ion channels are usually camer proteins With channel protein class, some ions need the process expending ATP to be Active transport, it is not necessary to expend energy and just belong to free diffusing, But condition is to the process of low concentration by high concentration, but usually, the associativity of channel protein is more weak, the flowing of liquid matrix Property is big, it is difficult to gathering and intercept and capture ion, cause a big obstacle of absorption process, the trophic structure being different from soil is the most fixing, There is not problem above, but soil matrix has a problem in that after fatting up absorption near root system, zonal nutrition can be caused Cavity, there is not mobility in soil.

Affect the factor of Nutrient Absorption mainly by the concentration of substrate nutrient, temperature, the long intensity of light, soil moisture, ventilation feelings Condition, soil pH, the physicochemical property of nutrient ion, the metabolic activity of root, seedling age, growthdevelopmental stage and the situation etc. of plant self nutrient Deng.

Summary of the invention

It is an object of the invention to: study the hydroponics substrate of a kind of liquid, it is provided that the nutritious unit of plant growth Element and growth activity material, increase operation rate, and solves that conventional matrix flow is good but element poor adhesive force, aeration are low causes Rot and pest problem, realize particular by following methods:

The high-efficient culture liquid of a kind of liquid plantation Fructus Cucumidis sativi, it is characterised in that be made up of the raw material of following weight (part): algae mixes Solution 5000 ~ 6000, potassium ferrate 6 ~ 10, aluminum sulfate 0.5 ~ 1.0, Herba Artemisiae Annuae slag 45 ~ 60, sodium citrate 3 ~ 6, manganese sulfate 6 ~ 9, breast Acid 6 ~ 10, calcium ammonium nitrate 10 ~ 14, potassium nitrate 4 ~ 6, EM microbial inoculum 1 ~ 3, magnesium sulfate 5 ~ 10, carbamide 20 ~ 25, bean cake powder 25 ~ 35, jade Rice core 30 ~ 45, Kaolin 10 ~ 16 and appropriate water；

Described algae mixed solution be mixed weight (part) be the glucose 10 ~ 15 of unit, starch 30 ~ 45, potassium dihydrogen phosphate 6 ~ 9, sodium selenite 1 ~ 3, ammonium molybdate 0.5 ~ 1.0, potassium permanganate 3 ~ 6, Fructus Hordei Germinatus powder 10 ~ 15, humic acid powder 3 ~ 6, carbamide 3 ~ 5, beef Cream 3 ~ 6, joins and makes culture fluid in water 400 ~ 500, and mixing is containing the Alga Sgrgassi Enerves of equal weight proportions, Chlorococcum, the algae that quivers, grid row 3 ~ 5 parts of the frustule powder of algae, Alga Sgrgassi Enerves and cyanophyceae, add 20 ~ dilution of the water of 30 times of its total weight parts after be inoculated into aforementioned culture fluid In, regulating pH7.0 ~ 7.5, temperature 15 ~ 20 DEG C also uses electric filament lamp prolonged exposure, is passed through filtrated air shaken cultivation 20 ~ 25 days, The concentration of detection alga cells is 3.5 × 10⁸Obtain after individual/more than L.

The preparation method of high-efficient culture liquid of a kind of liquid plantation Fructus Cucumidis sativi, it is characterised in that include following step:

(1) potassium ferrate is added in the water of 10 ~ 15 times of its weight portions, add after stirring and dissolving to algae mixed solution, with The rotating speed stirring 20 ~ 30min of 200 ~ 250r/ min, obtains flocculence colloidal solution；

(2) mixing bean cake powder, corn cob, Kaolin, Herba Artemisiae Annuae slag, appropriate water spray, to water content 50 ~ 55%, inoculates EM microbial inoculum, insulation 35 ~ 40 DEG C, heap fermentation 18 ~ 22 days, within every 2 ~ 3 days, stir once, high temperature sterilize add water dilute by material-water ratio 1:15 ~ 20 after completing Releasing, obtain supernatant, join in the colloidal solution described in (1) after being centrifuged, stirring and evenly mixing obtains solution A；

(3) mixing sodium citrate, manganese sulfate, lactic acid and other following residual components being not directed to, add to reactor, add The water of 15 ~ 20 times, is heated to 65 ~ 70 DEG C of stirring and dissolving and obtains solution B；

(4) mixed solution A and solution B, be simultaneously introduced aluminum sulfate coagulant, with 200 ~ 250min rotating speed stirring 2 ~ 4min after with 40 ~ 50r/min continues stirring 10 ~ 15min, after gained colloidal solution and water are finally pressed the dilution proportion of 1:10 ~ 15.

The present invention is according to problem existing in liquid matrix, and purport thinking is that research one is present in soilless culture root of the crop A kind of medium between system and liquid matrix, by this medium, can be with " bridge " between effective connection root system and substrate, this On the one hand kind intermediate can be combined into flocculence material and be combined in the vicinity of root system, can adsorb and draw in water again simultaneously Inorganic salt, the root system made can intercept and capture required nutrient in the liquid matrix of high fluidity smoothly,

Research finds that the alga cells in water there occurs the change of structure and form after ferrate oxidation, reduces algae thin The activeness of born of the same parents, and add the precipitability of frustule, Ferrate Pre-Oxidation Positive rear section frustule is assembled.Ferrate Pre-oxidation feature be decompose after can be formed ferric hydroxide colloid precipitation, ferric hydroxide colloid is deposited to the feelings on alga cells surface Condition, these precipitations obviously can change the surface nature of algae, such as the surface electrical behavior of frustule, destroys the surface texture of frustule, Cause the winding of frustule surface sheath, and the epitheca of cell may be made to ftracture, cause intracellular organic matter to outflow.Ferrate oxidation The intracellular organic matter release of the alga cells caused necessarily makes the Organic substance degree in water or quantity change, and discharges in water Chitin fiber, additionally alga cells is also possible to other organic substances discharged in addition to chitin, these chlorellas or indigo plant The intracellular organic matter that chlorella discharges can have the character of electronegative or neutral polyelectrolyte in water, and therefore has one Fixed flocculation.

Alga cells ruptures the intracellular organic matter of release such as simultaneously: chitinase, protease inhibitor, Bt endotoxin protein, Antibacterial secondary metabolites, fungus have extremely strong anti-insect activity about organized enzyme etc., can also add in right amount in the process cultivated Trace element, utilizes the absorbing activity of alga cells to absorb, is converted into the organic trace element form of combined state, it is simple to crop Absorb, promote the utilization of nutrient.

Beneficial effects of the present invention: according to nutritional need during green cucumber, add appropriate nutrient, be dissolved in Algae ruptures in the culture fluid that cell is substrate, and this is the solution of a kind of colloid, and flow velocity stirs relatively, has and promotes Nutrient Absorption Function, is greatly saved human resources and land resource, it is achieved high yield, simultaneously because need not earth, so reducing due to disease pest The unnecessary loss that evil causes, does not the most use pesticide, safety and sanitation.

Detailed description of the invention

Embodiment 1:

The high-efficient culture liquid of a kind of liquid plantation Fructus Cucumidis sativi, it is characterised in that be made up of the raw material of following weight (g): algae mixes Solution 5400, potassium ferrate 7, aluminum sulfate 0.6, Herba Artemisiae Annuae slag 55, sodium citrate 5, manganese sulfate 8, lactic acid 9, calcium ammonium nitrate 12, nitric acid Potassium 4, EM microbial inoculum 2, magnesium sulfate 7, carbamide 22, bean cake powder 28, corn cob 38, Kaolin 12 and appropriate water；

Described algae mixed solution be mixed weight (Kg) be the glucose 14 of unit, starch 40, potassium dihydrogen phosphate 7, Monohydrated selenium dioxide Sodium 1, ammonium molybdate 0.6, potassium permanganate 5, Fructus Hordei Germinatus powder 12, humic acid powder 4, carbamide 3, Carnis Bovis seu Bubali cream 5, join in water 450 and make training Nutrient solution, mixing, containing the Alga Sgrgassi Enerves of equal weight proportions, Chlorococcum, the algae that quivers, scenedemine, Alga Sgrgassi Enerves and 4 parts of the frustule powder of cyanophyceae, adds Being inoculated in aforementioned culture fluid after the water dilution of 20 ~ 30 times of its total weight parts, regulate pH7.0 ~ 7.5, temperature 15 ~ 20 DEG C also uses Electric filament lamp prolonged exposure, is passed through filtrated air shaken cultivation 20 ~ 25 days, and the concentration of detection alga cells is 3.5 × 10⁸Individual/L with Obtain after on.

The preparation method of high-efficient culture liquid of a kind of liquid plantation Fructus Cucumidis sativi, it is characterised in that include following step:

(1) potassium ferrate is added in the water of 10 ~ 15 times of its weight portions, add after stirring and dissolving to algae mixed solution, with The rotating speed stirring 20 ~ 30min of 200 ~ 250r/ min, obtains flocculence colloidal solution；

(2) mixing bean cake powder, corn cob, Kaolin, Herba Artemisiae Annuae slag, appropriate water spray, to water content 50 ~ 55%, inoculates EM microbial inoculum, insulation 35 ~ 40 DEG C, heap fermentation 18 ~ 22 days, within every 2 ~ 3 days, stir once, high temperature sterilize add water dilute by material-water ratio 1:15 ~ 20 after completing Releasing, obtain supernatant, join in the colloidal solution described in (1) after being centrifuged, stirring and evenly mixing obtains solution A；

(3) mixing sodium citrate, manganese sulfate, lactic acid and other following residual components being not directed to, add to reactor, add The water of 15 ~ 20 times, is heated to 65 ~ 70 DEG C of stirring and dissolving and obtains solution B；

(4) mixed solution A and solution B, be simultaneously introduced aluminum sulfate coagulant, with 200 ~ 250min rotating speed stirring 2 ~ 4min after with 40 ~ 50r/min continues stirring 10 ~ 15min, after gained colloidal solution and water are finally pressed the dilution proportion of 1:10 ~ 15.

Using the liquid culture fluid nutritional solution for soilless culture Fructus Cucumidis sativi of the present invention, matched group selects conventional nutrient liquid, The preventing of other daily management and pest and disease damage takes the circumstances into consideration to implement according to practical situation, and result proves: nutritional solution of the present invention can delay The ageing time of Fructus Cucumidis sativi 6 ~ 8 days, adds fresh and tender degree and the mouthfeel of Fructus Cucumidis sativi, and pest and disease damage incidence rate reduces, and improves economic well-being of workers and staff 20.3%。

Claims (2)

1. the high-efficient culture liquid of a liquid plantation Fructus Cucumidis sativi, it is characterised in that be made up of the raw material of following weight (part): algae mixes Close solution 5000 ~ 6000, potassium ferrate 6 ~ 10, aluminum sulfate 0.5 ~ 1.0, Herba Artemisiae Annuae slag 45 ~ 60, sodium citrate 3 ~ 6, manganese sulfate 6 ~ 9, Lactic acid 6 ~ 10, calcium ammonium nitrate 10 ~ 14, potassium nitrate 4 ~ 6, EM microbial inoculum 1 ~ 3, magnesium sulfate 5 ~ 10, carbamide 20 ~ 25, bean cake powder 25 ~ 35, Corn cob 30 ~ 45, Kaolin 10 ~ 16 and appropriate water；

Described algae mixed solution be mixed weight (part) be the glucose 10 ~ 15 of unit, starch 30 ~ 45, potassium dihydrogen phosphate 6 ~ 9, sodium selenite 1 ~ 3, ammonium molybdate 0.5 ~ 1.0, potassium permanganate 3 ~ 6, Fructus Hordei Germinatus powder 10 ~ 15, humic acid powder 3 ~ 6, carbamide 3 ~ 5, beef Cream 3 ~ 6, joins and makes culture fluid in water 400 ~ 500, and mixing is containing the Alga Sgrgassi Enerves of equal weight proportions, Chlorococcum, the algae that quivers, grid row 3 ~ 5 parts of the frustule powder of algae, Alga Sgrgassi Enerves and cyanophyceae, add 20 ~ dilution of the water of 30 times of its total weight parts after be inoculated into aforementioned culture fluid In, regulating pH7.0 ~ 7.5, temperature 15 ~ 20 DEG C also uses electric filament lamp prolonged exposure, is passed through filtrated air shaken cultivation 20 ~ 25 days, The concentration of detection alga cells is 3.5 × 10⁸Obtain after individual/more than L.

The preparation method of the high-efficient culture liquid of a kind of liquid plantation Fructus Cucumidis sativi, it is characterised in that include Following step:

(1) potassium ferrate is added in the water of 10 ~ 15 times of its weight portions, add after stirring and dissolving to algae mixed solution, with The rotating speed stirring 20 ~ 30min of 200 ~ 250r/ min, obtains flocculence colloidal solution；

(2) mixing bean cake powder, corn cob, Kaolin, Herba Artemisiae Annuae slag, appropriate water spray, to water content 50 ~ 55%, inoculates EM microbial inoculum, insulation 35 ~ 40 DEG C, heap fermentation 18 ~ 22 days, within every 2 ~ 3 days, stir once, high temperature sterilize add water dilute by material-water ratio 1:15 ~ 20 after completing Releasing, obtain supernatant, join in the colloidal solution described in (1) after being centrifuged, stirring and evenly mixing obtains solution A；

(3) mixing sodium citrate, manganese sulfate, lactic acid and other following residual components being not directed to, add to reactor, add The water of 15 ~ 20 times, is heated to 65 ~ 70 DEG C of stirring and dissolving and obtains solution B；

(4) mixed solution A and solution B, be simultaneously introduced aluminum sulfate coagulant, with 200 ~ 250min rotating speed stirring 2 ~ 4min after with 40 ~ 50r/min continues stirring 10 ~ 15min, after gained colloidal solution and water are finally pressed the dilution proportion of 1:10 ~ 15.