CN106116030B - A kind of method of quinoline pollutant in treated sewage - Google Patents
A kind of method of quinoline pollutant in treated sewage Download PDFInfo
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- CN106116030B CN106116030B CN201610600496.5A CN201610600496A CN106116030B CN 106116030 B CN106116030 B CN 106116030B CN 201610600496 A CN201610600496 A CN 201610600496A CN 106116030 B CN106116030 B CN 106116030B
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- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 title claims abstract description 88
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000010865 sewage Substances 0.000 title claims abstract description 19
- 239000003344 environmental pollutant Substances 0.000 title abstract description 9
- 231100000719 pollutant Toxicity 0.000 title abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 11
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 239000002351 wastewater Substances 0.000 claims description 10
- -1 quinoline compound Chemical class 0.000 claims description 9
- 150000001413 amino acids Chemical class 0.000 claims description 7
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 6
- 239000010786 composite waste Substances 0.000 claims description 6
- 230000035772 mutation Effects 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 238000003672 processing method Methods 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000007667 floating Methods 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 239000002574 poison Substances 0.000 claims 1
- 231100000614 poison Toxicity 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 230000000694 effects Effects 0.000 description 10
- 230000015556 catabolic process Effects 0.000 description 7
- 238000006731 degradation reaction Methods 0.000 description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000004939 coking Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000589776 Pseudomonas putida Species 0.000 description 2
- 208000031320 Teratogenesis Diseases 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- WHRZCXAVMTUTDD-UHFFFAOYSA-N 1h-furo[2,3-d]pyrimidin-2-one Chemical compound N1C(=O)N=C2OC=CC2=C1 WHRZCXAVMTUTDD-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 235000006173 Larrea tridentata Nutrition 0.000 description 1
- 244000073231 Larrea tridentata Species 0.000 description 1
- 102000008300 Mutant Proteins Human genes 0.000 description 1
- 108010021466 Mutant Proteins Proteins 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920000388 Polyphosphate Polymers 0.000 description 1
- 229920000037 Polyproline Polymers 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940111121 antirheumatic drug quinolines Drugs 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 239000003034 coal gas Substances 0.000 description 1
- 239000011280 coal tar Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229960002126 creosote Drugs 0.000 description 1
- 238000005235 decoking Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000002803 fossil fuel Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229960003540 oxyquinoline Drugs 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000001205 polyphosphate Substances 0.000 description 1
- 235000011176 polyphosphates Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 150000003248 quinolines Chemical class 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/305—Nitrification and denitrification treatment characterised by the denitrification
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
- C12Y207/04001—Polyphosphate kinase (2.7.4.1)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/308—Biological phosphorus removal
Abstract
The invention discloses a kind of method of quinoline pollutant in treated sewage, including pretreatment, main process task is aerated, and post-treating and other steps can remove most quinoline pollutant after treatment.The present invention has very important actual application value to the improvement of quinoline pollutant.
Description
Technical field
The invention belongs to sewage treatment fields, and in particular to the processing of quinoline pollutant in sewage.
Background technology
Quinoline is a kind of colourless, the hygroscopic liquid with penetrating odor.Dissolve in ethyl alcohol, ether, benzene, curing
Carbon is slightly soluble in water.From the point of view of its chemical constitution, it belongs to heterocyclic arene type organic, by shape when coal, timber, PETROLEUM PROCESSING
At.Quinoline in coking wastewater then mostly generates during decoking oil wash benzene.The pollution of quinoline is mainly by air, water body, ground
The modes such as lower coal mining are propagated, the also ingredient containing quinoline in the gas of cigarette discharge.Some researchers, which study, finds liver
Dirty is the target organ of quinoline, is had a certain impact to liver cancer.
Quinoline is typical polycyclic aromatic nitrogen-contg jeterocyclic chemistry compound, has big toxicity, teratogenesis and carcinogenicity strong etc. special
Point, it is difficult to it is biodegradable, there can be huge potential danger to human health and ecological environment by soil pollution groundwater resources
Evil, increasingly causes the concern of people.Quinoline is the Typical Representative of nitrogen-containing heterocycle compound, and purposes is extremely extensive, is a variety of doctors
Important source material in the productions such as medicine, agricultural product, dyestuff and solvent.But quinoline and its derivates can carcinogenic, teratogenesis, mutagenesis,
And it is difficult to biodegradation, it is the severe pollutant in environment.Quinoline in environment is essentially from coal gas, fossil fuel processing, coal
Tar residue and wood preservation facility.In addition, quinoline and its derivates or coking wastewater, petroleum wastewater, pharmacy waste water etc.
Persistent organic pollutants ingredient in various industrial sewage.Progress is formed to the organic matter of 2 kinds of coking wastewaters by scholar respectively
Analysis, it is indicated that the content of quinolines occupies the second of organic pollution.Since quinoline contains the very strong nitrogen of I electronegativity
Atom makes its water solubility enhancing, therefore this kind of compound is more easy to spread in the environment and persistently exist.Existing investigation is found
Quinoline and its oxyquinoline at concentrations up to several mg/L in underground water and soil near creosote polluted place.Except this it
Outside, quinoline and its derivates are also present in urban air, tobacco smoke, seawater and the tissue of fish, therefore are found and effectively removed
The method of quinoline has a very important significance.
Now, there is the processing method of many quinoline.It is roughly divided into Physical, chemical method and bioanalysis.Physical and change
Although method removal efficiency is high, of high cost, secondary pollution is easy tod produce.Biological treating have at low cost, secondary pollution it is light,
The advantages such as Environmental compatibility is good, have become one of the preferred option in pollutant abatement technology.The success of decision biochemical processing process,
Effectively, applicable factor, other than process conditions and operational administrative, the functional gene for processes such as contaminant degradation, conversions
Important function be also obvious.Biological treatment is because it is big with treating capacity, cost is relatively low, mild condition and does not generate
The advantages that secondary pollution and have been favored by people.In recent years, pass through specific work(on the basis of not changing existing treatment facility
The biological reinforcing technology of the addition of energy microorganism can greatly improve original biological treatment system and be imitated to the removal of target contaminant
Fruit, sewage treatment capacity greatly improve, thus are increasingly valued by people.
Domestic and international researcher begins to the microorganism of separation degradation quinoline from the 1970s.Microorganism in relation to quinoline
The research of degradation pathway and its degradation kinetics, science of heredity is also being goed deep into, but up to the present, the biodegradation of such compound
Process study is still fairly limited, and then less for other functional studies of quinoline-degrading bacterium.
Invention content
Method for waste water containing quinoline being handled using biotechnology one of the objects of the present invention is to provide a kind of, to solve waste water
In pollutant containing quinoline the problem of being difficult to biodegradable and detoxification.
The present invention adopts the following technical scheme that realization:
A kind of processing method of the waste water containing quinoline compound, includes the following steps:
(1) it pre-processes:By filtering and precipitating, the suspended matter in handled sewage is removed；
(2) main process task:Nitrogen oxide is added, composite waste is made into, is collected in intake pool, wherein carbon-nitrogen ratio 5-
10:1, the carbon in carbon-nitrogen ratio comes from quinoline compound, nitrogen comes from the nitrogen oxide of addition；
(3) composite waste is promoted to by intake pump in upflow type aerating biological filter, oxygen supply aeration 6h；
(4) sewage after being aerated is transferred to anoxic zone, adds the floating stuffing for carrying denitrifying bacterium, handles 1 day；
(5) it post-processes:By treated for anoxic zone sewage it is sterile-processed after, direct emission or reuse.
Preferably, the denitrifying bacterium is to be transferred to any one following PPKL1 protein variant of coding (relative to PPKL1
Albumen original amino acid (its amino acid sequence referring to：GenBank：NP_742191.1 following mutation 60E/) is carried out respectively
R, 69F/P, 82P/G, 120I/F, 147L/A, 178R/S, 189E/V, 229I/Q, 243V/Q, 251S/E, 259E/P) (60E/R
Indicate that in the E amino acid substitutions of original series the 60th be R amino acid, other meanings are similar) encoding gene CCTCC M
2010209 bacterial strains.
The present invention additionally provides a kind of quinoline-degrading albumen PPKL1, amino acid sequence such as NP_742191.1.The albumen
It is that the differential protein expression induced by quinoline from pseudomonas putida screens.The albumen has stronger removal point
Solve the ability of quinoline.
The present invention provides a series of PPKL1 protein variants (relative to PPKL1 albumen original amino acid respectively into
Row following mutation 60E/R, 69F/P, 82P/G, 120I/F, 147L/A, 178R/S, 189E/V, 229I/Q, 243V/Q, 251S/E,
259E/P), the ability of quinoline is decomposed with stronger removal.The coded sequence of the variant amino acids by shown in is in CCTCC M
When being expressed in 2010209 bacterial strains, it is found that recombinant bacterium relative to the polyP bacteria of the unconverted gene there is preferably removal to decompose
The ability of quinoline has better effect.
The albumen is imported into denitrifying bacterium, which not only has the effect of preferably going to denitrogenate phosphorus, while can also
Quinoline in enough effective removal sewage, has preferable application prospect.
The above method has the advantages that:
1, processing method through the invention substantially increases the degradation rate of quinoline, reduces the reaction time.
2, a kind of albumen and conversion bacterial strain of degradation quinoline are provided, there are higher quinoline removal efficiency and nitrogen phosphorus ligands
Effect.
In order to make it easy to understand, the present invention will be described in detail by specific embodiment below.It needs to refer in particular to
Go out, specific example is merely to illustrate, and be not meant to limit the scope of the invention.The ordinary skill people of obvious this field
Member can make various modifications and variations according to illustrating herein to the present invention within the scope of the invention, these amendments
It is also included in the scope of the present invention with changing.
Specific implementation mode
The processing of 1 quinoline compound waste water of embodiment
(1) it pre-processes:It takes and is filtered by conventional filtering ponds containing a concentration of 100mg/L sewage of quinoline, by normal
The sedimentation basin of rule is precipitated, and the suspended matter in handled sewage is removed；
(2) main process task:It will eliminate suspended matter treated sewage, addition nitrogen oxide (if nitrogen content is enough,
Addition can not also be used), it is made into composite waste, is collected in intake pool, wherein carbon-nitrogen ratio 8:1, the carbon in carbon-nitrogen ratio comes from
Come from the nitrogen oxide of addition in quinoline compound, nitrogen；
(3) composite waste is promoted to by intake pump in upflow type aerating biological filter, oxygen supply aeration 6h；
(4) sewage after being aerated is transferred to anoxic zone, adds the floating stuffing for carrying denitrifying bacterium, handles 1 day；It is described anti-
Nitrifier is the bacterial strain that culture presevation number is CCTCC M 2010209, specifically may refer to CN102115719A；
(5) it post-processes:By treated for anoxic zone sewage it is sterile-processed after, by measuring, wherein quinoline is a concentration of
0.5mg/L。
The acquisition of 2 quinoline-degrading gene PPKL1 of embodiment
Pseudomonas putida is taken, using quinoline as derivant, carries out Fiber differentiation, respectively the bacterium to induce and not induce
Strain as a comparison, carries out the extraction of total protein, carries out bidirectional two-dimensional electrophoresis, passes through control, it was found that differentially expressed protein at 10.
The larger point of differential expression at wherein one is wherein chosen to be sequenced.Corresponding amino acid sequence is obtained, sequence is such as
Genbank：Shown in NP_742191.1.The gene is carried out to conventional prokaryotic expression, specific steps are all conventional technique.It is logical
Determination of activity discovery is crossed, which has stronger quinoline removal effect.
The activity of embodiment 3PPKL1 protein mutations improves
By multiplex PCR by corresponding 60E/R, 69F/P, 82P/G, 120I/F, 147L/A, 178R/S, 189E/V,
229I/Q, 243V/Q, 251S/E, 259E/P, 121E/S, mutational site are introduced between in albumen shown in NP_742191.1, from
And construct different mutant protein (preparation method that can refer to the prior art can be obtained).It prepares and obtains different prominent
Then variant gene is connected respectively at carrier, the recombinant plasmid transformed being proved to be successful is entered denitrifying bacterium CCTCC M
In 2010209, the genetic engineering bacterium of degradation quinoline is obtained.
The verification of 4 quinoline-degrading effect of embodiment
Multiple engineering bacterias that embodiment 3 is obtained are respectively according to the method for embodiment 1, respectively under different quinoline concentration
The effect of the corresponding degradation quinoline of verification.As a contrast with the Ep2 bacterium of CN 102206657A.Concrete outcome is as shown in the table：
The denitrifying bacterium that can be seen that the albumen PPKL1 for having imported mutation from above result is most of all with enhancing
Removal quinoline effect, this absolutely prove is not arbitrary change all have preferable effect.
Claims (2)
1. a kind of processing method of waste water containing quinoline compound, includes the following steps:(1) it pre-processes:By filtering and precipitating, go
Except the suspended matter in handled sewage；(2) main process task:Nitrogen oxide is added, composite waste is made into, is collected in intake pool,
Wherein, carbon-nitrogen ratio 5-10:1, the carbon in carbon-nitrogen ratio comes from quinoline compound, nitrogen comes from the nitrogen oxide of addition；(3)
Composite waste is promoted to by intake pump in upflow type aerating biological filter, oxygen supply aeration 6h；(5) sewage after being aerated is transferred to scarce
Oxygen area adds the floating stuffing for carrying denitrifying bacterium, handles 1 day；(6) it post-processes:By treated for anoxic zone sewage through disappearing
After poison processing, direct emission or reuse, the denitrifying bacterium are the encoding gene for being transferred to the following PPKL1 protein variants of coding
2010209 bacterial strains of CCTCC M, the PPKL1 protein variants be relative to PPKL1 albumen original amino acids carry out such as
Lower mutation 82P/G, PPKL1 albumen original amino acid is referring to GenBank：NP_742191.1.
2.PPKL1 protein variants, it is characterised in that：The PPKL1 protein variants are relative to PPKL1 albumen Original amino sequences
Row carry out following mutation 82P/G, and PPKL1 albumen original amino acids are referring to GenBank：NP_742191.1.
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| CN105967467A (en) | 2016-09-28 |
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| CN106116030A (en) | 2016-11-16 |
| CN104944700A (en) | 2015-09-30 |
| CN106145524A (en) | 2016-11-23 |
| CN106007251B (en) | 2019-02-15 |
| CN106145523A (en) | 2016-11-23 |
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